Anatomy and Regenerative Medicine: From Methods to Applications (2nd Edition)

A special issue of Applied Biosciences (ISSN 2813-0464).

Deadline for manuscript submissions: 30 November 2025 | Viewed by 1208

Special Issue Editors


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Guest Editor
Department of Biomedicine, Neurosciences and Advanced Diagnostic (BiND), Human Anatomy Section, University of Palermo, Via del Vespro 129, 90127 Palermo, Italy
Interests: tissue engineering; human anatomy; stem-cell therapies
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Guest Editor
Department of Theoretical and Applied Sciences, eCampus University, 22060 Novedrate, Italy
Interests: artificial organs; bioengineering; regenerative medicine; tissue engineering; biomaterials
Special Issues, Collections and Topics in MDPI journals

E-Mail Website
Guest Editor
Department of Biomedicine, Neurosciences and Advanced Diagnostic (BiND), Human Anatomy Section, University of Palermo, Via del Vespro 129, 90127 Palermo, Italy
Interests: human anatomy; biomedical engineering; 3D cell cultures
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Anatomy and regenerative medicine (RM) are two strongly interconnected disciplines. The promising field of regenerative medicine may be defined as replacing or "regenerating" human cells, tissues, or organs to restore or establish normal functions. Beginning from the basics provided by human anatomy remains the best approach. Years of studies of and insights into the composition of the human body offer a solid starting ground on which to develop new therapeutic paths. Macro-anatomy data contribute to the replacement/healing of entire organs, and nanotechnology uses micro-anatomy discoveries.

RM can offer a modern solution to existing long-term problems. There are many application fields, from stem-cell therapy to tissue engineering, from biomaterial 3D printing to artificial organs.

These applications' achievements often result from collaboration with scientists outside of the clinical area (bioengineers, materials engineers, biologists).

This Special Issue of Applied Biosciences, "Anatomy and Regenerative Medicine: From Methods to Applications (2nd Edition)", is committed to all discoveries and applications of RM. Research papers emphasizing the shift from anatomical data to practical applications will be particularly appreciated.

Dr. Alessandro Pitruzzella
Dr. Alberto Fucarino
Prof. Dr. Fabio Bucchieri
Guest Editors

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Keywords

  • human anatomy
  • regenerative medicine
  • nanotechnology
  • bioengineering
  • stem cell therapies
  • tissue engineering
  • artificial organs
  • biomaterials
  • artificial intelligence

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Research

28 pages, 7778 KB  
Article
Evaluation of the Effects of Demineralization and Decellularization for the Development of a Decellularized Bone Matrix from Tuna (Thunnus albacares) Bone
by Zesreal Cain Bantilan, Marionilo Labares, Jr., Johnel Alimasag, Kit Dominick Don Valle, Chancy Louisse Barlisan, Elizer Eleccion and Ronald Bual
Appl. Biosci. 2025, 4(3), 43; https://doi.org/10.3390/applbiosci4030043 - 12 Sep 2025
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Abstract
The exploration of alternative sources of extracellular matrix (ECM) is driven by increasing demand and the need for sustainable biomaterials. Tuna (Thunnus albacares) bones, a by-product of the fishing industry, represent a potential ECM source due to their abundance, collagen-rich composition, [...] Read more.
The exploration of alternative sources of extracellular matrix (ECM) is driven by increasing demand and the need for sustainable biomaterials. Tuna (Thunnus albacares) bones, a by-product of the fishing industry, represent a potential ECM source due to their abundance, collagen-rich composition, and biocompatibility. This study investigated the effects of demineralization using hydrochloric acid (HCl) at concentrations of 0.5 M and 1.0 M for varying durations (0.5, 1, 6, 12, and 24 h), and decellularization using sodium dodecyl sulfate (SDS) and Triton X-100 (TX100) at concentrations of 0.1%, 0.5%, and 1.0%. Demineralization effectively reduced inorganic content, with residual calcium levels dropping below 5% after 6 h. Kinetic analysis indicated a second-order reaction, and ATR–FTIR spectra confirmed the disappearance of phosphate and carbonate peaks alongside the preservation of amide bands. Demineralized bone matrix (dBM) retained good thermal stability and mechanical properties. Decellularization efficiency was assessed through H&E staining, dsDNA quantification, and SDS-PAGE analysis. Decellularized extracellular bone matrix (dEBM) treated with 1.0% SDS showed the lowest dsDNA levels (14.00 ± 7.94 ng/mg) and absence of cellular material. SDS-PAGE confirmed type I collagen preservation, particularly in samples treated with ≤0.5% SDS or TX100. This study establishes that tuna fish bones can be effectively processed into ECM, supporting their potential as a sustainable biomaterial for advanced biomedical applications. Full article
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