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Applied Biosciences, Volume 5, Issue 4

2026 December - 33 articles

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Articles (33)

  • Article
  • Open Access

Plant-Assisted Synthesis of Nd-Doped ZnO Nanoparticles—Characterization and In Vitro Antimicrobial Assay

  • Irina Lazarkevich,
  • Katerina Zaharieva,
  • Mariela Dimitrova,
  • Petya Todorova and ,
  • Ralitsa Mladenova,
  • Maria Shipochka,
  • Ognian Dimitrov,
  • Rumyana Eneva,
  • Stephan Engibarov and
  • Irina Stambolova
  • + 8 authors+ 3 authors

Green-synthesized pure and Neodymium-doped zinc oxide nanoparticles (ZnO NPs and Nd-doped ZnO NPs) were prepared using Vaccinium vitis-idaea L. (Ericaceae) leaf extract via a hydrothermal route. The structural, morphological, and magnetic characteris...

  • Article
  • Open Access

Evaluation of the Honey Bee Brood Humidity Rearing Protocol in Laboratory Conditions for Ecotoxicological Testing in Apis mellifera iberiensis

  • Felipe López-Villegas,
  • José L. Ávila-Jiménez,
  • Francisco J. Rodríguez-Lozano and
  • José M. Flores

Apis mellifera is a key species in ecotoxicological assessments of pest control products. OECD and EFSA guidelines include bee brood toxicity tests, but these protocols are largely based on central and northern European bees. A. mellifera iberiensis,...

  • Article
  • Open Access
12 Views
20 Pages

Effects of Foliar Boron, Molybdenum, and Sulfur Applications on Yield and Forage Quality of Alfalfa

  • Zübeyir Ağirağaç,
  • Mehmet Salih Kaçmaz and
  • Şeyda Zorer Çelebi

This study investigated the effects of foliar applications of boron (B), molybdenum (Mo), and sulfur (S) on forage yield and quality in two alfalfa (Medicago sativa L.) cultivars, Bilensoy and Savaş. Four treatments (control, MB, S, and MB+S) we...

  • Review
  • Open Access
10 Views
30 Pages

Genodermatoses: Current Strategies for Disease Modeling and Prospects

  • A. S. Ponomarev,
  • R. R. Norkin,
  • L. A. Khusainova,
  • L. M. Yanygina and ,
  • M. D. Drugova,
  • V. V. Solovyeva and
  • A. A. Rizvanov
  • + 2 authors+ 0 author

Genodermatoses are rare hereditary skin disorders, most of which still lack effective curative treatment, and progress toward modern therapy depends on experimental models that capture both the genetic defect and its tissue context. This review exami...

(This article belongs to the Special Issue Anatomy and Regenerative Medicine: From Methods to Applications (3rd Edition))
  • Review
  • Open Access
37 Views
26 Pages

Non-thermal processing technologies: high-pressure processing (HPP), pulsed electric fields (PEF), ultrasound (US), and cold plasma (CP), are increasingly investigated as tools for food matrix modification and preservation. This structured critical n...

(This article belongs to the Special Issue Functional Food: Molecular Nutrition, Emerging Technologies and Applications)
  • Article
  • Open Access
62 Views
23 Pages

Effects of a Mixed Bile-Acid Preparation on Growth Performance, Digestive Enzyme Activities, Gut and Liver Histology, and Expression of Lipid Metabolism-Related Genes in Asian Catfish (Clarias batrachus)

  • Srinibas Das,
  • Ayan Mukherjee,
  • Samiran Mondal,
  • Indrajit Kar and ,
  • Paresh Nath Chatterjee,
  • Sukalpa Mandal,
  • Pratyayee Kar,
  • Kumaresh Soren,
  • Sukhen Samanta and
  • Guru Prasad Mandal
  • + 6 authors+ 1 author

Bile acids (BAs) exert a dose-dependent, biphasic effect on growth performance in various fish species, acting as hepatoprotective and trophic agents at moderate levels, while becoming cytotoxic at higher concentrations. This study was conducted to a...

  • Article
  • Open Access
51 Views
28 Pages

Enset (Ensete ventricosum (Welw.) Cheesman) is an important perennial food crop that contributes to food security and has potential for climate-resilient agriculture. Its diverse germplasm represents an important genetic resource for conservation and...

  • Article
  • Open Access
156 Views
20 Pages

Laboratory Evaluation of Native and Commercial Entomopathogenic Fungi Against Macrodactylus nigripes (Coleoptera: Melolonthidae) Associated with Maize Crops

  • Omar Kareem Martínez Bonilla,
  • Omar Romero-Arenas,
  • Conrado Parraguirre Lezama,
  • Raúl Ávila Sosa and ,
  • Rolando Rueda Luna,
  • Ricardo Munguía Pérez and
  • Manuel Huerta Lara
  • + 2 authors+ 0 author

Maize in Mexico suffers severe economic losses due to root-feeding and defoliating pests, among which Macrodactylus nigripes is particularly damaging, attacking roots during its larval stage and reducing pollination in adults. The intensive use of ch...

(This article belongs to the Topic Sustainable Environmental Technologies—2nd Edition)
  • Review
  • Open Access
112 Views
59 Pages

Multi-omics is a field that studies the interactions between different biological modalities to obtain a more precise medical understanding of biological phenomena. With recent advances in high-throughput technologies, multi-omics datasets have becom...

(This article belongs to the Special Issue Neural Networks and Deep Learning for Biosciences, 2nd Edition)
  • Article
  • Open Access
98 Views
17 Pages

Assessment of Microbial Contamination in Stagnant Water from Dental Unit Reservoirs

  • Isabelly Karina Missi de Souza,
  • Gabriel Paranhos Tanaka,
  • Letícia de Souza Moda-Monteiro,
  • Amanda Carolina Souza Delfino da Rocha and ,
  • Tatiana Areas da Cruz,
  • Viviane de Cássia Oliveira and
  • Evandro Watanabe
  • + 2 authors+ 0 author

Water stagnation in dental unit reservoirs may be associated with increased microbial contamination and potential exposure to opportunistic microorganisms, particularly among medically vulnerable patients and dental healthcare professionals. This stu...

  • Review
  • Open Access
87 Views
22 Pages

In patients with diabetes, the concentration of glycated proteins increases. Among them is glycated hemoglobin (HbA1c), which is currently the standard biomarker for assessing long-term glycemic control. However, this parameter has certain limitation...

(This article belongs to the Special Issue Feature Reviews for Applied Biosciences)
  • Article
  • Open Access
131 Views
23 Pages

Distribution, Co-Carriage Patterns, and Applied Biostatistical Analysis of Virulence Determinants in Group B Streptococcus Isolates

  • Vasil S. Boyanov,
  • Alexandra S. Alexandrova,
  • Lyudmila Boyanova,
  • Hristina Y. Hitkova and
  • Raina T. Gergova

An analysis of the distribution and co-occurrence patterns of targeted virulence determinants in Streptococcus agalactiae (Group B Streptococcus; GBS) was conducted. This study employed a cost-efficient methodology that integrates PCR with statistica...

  • Review
  • Open Access
147 Views
25 Pages

Gallic Acid Bioproduction: From Tannin Hydrolysis to De Novo Biosynthesis by Engineered Microbial Cell Factories

  • Raoní K. Pantoja,
  • Matheus M. Torres,
  • Amanda S. Santos,
  • Edmar R. Oliveira-Filho and ,
  • José G. C. Gomez and
  • Luiziana F. Silva
  • + 1 author+ 0 author

Gallic acid (GA) is a value-added phenolic compound widely applied in the food, pharmaceutical, and cosmetic industries. Traditionally obtained through chemical hydrolysis of tannins, its production faces limitations related to environmental impact,...

(This article belongs to the Special Issue Applications of Microorganisms and Enzymes in the Production of Fuels and Chemicals)
  • Review
  • Open Access
203 Views
34 Pages

Supplementation Strategies to Improve Vitrification-Induced Cryodamage in Oocytes and Embryos: A Narrative Review

  • Shravya Shetty,
  • Mouna Bannur Karunakara,
  • Preema Laveena Serrao,
  • Vanishree Vasave Madhvacharya and ,
  • Kristipati Ravi Ram,
  • Shama Prasada Kabekkodu,
  • Guruprasad Kalthur and
  • Sandhya Kumari
  • + 3 authors+ 0 author

Vitrification has revolutionized oocyte and embryo cryopreservation in assisted reproductive technology, enabling the preservation of female fertility. It safeguards the reproductive potential of women facing medical treatments or elective delay of c...

  • Article
  • Open Access
161 Views
41 Pages

Bridging Large Language Model–Based AI and Conventional Statistics for Retrospective Mortality Risk Assessment in Intra-Abdominal Sepsis and Septic Shock

  • Andreea Elena Stan,
  • Emilia Patrascanu,
  • Wael Jalloul,
  • Irina Anca Ristescu and ,
  • Mihaela Blaj,
  • Mihaela Breabăn,
  • Maria-Ecaterina Olariu,
  • Andreia-Florentina Corduneanu,
  • Adrian Iftene and
  • Cipriana Stefanescu
  • + 5 authors+ 0 author

Background: Severe intra-abdominal infections are a major cause of sepsis and septic shock in intensive care units (ICUs), with high mortality and heterogeneity. Identifying variables associated with mortality is essential for risk assessment in this...

(This article belongs to the Special Issue Neural Networks and Deep Learning for Biosciences, 2nd Edition)
  • Review
  • Open Access
196 Views
26 Pages

From Binding to Antibiotic Action: Phage Display as a Translational Platform for Antimicrobial Discovery, Antibiotic Rescue, and Precision Therapy

  • Abel Villa-Mancera,
  • José Manuel Robles-Robles,
  • Jaime Olivares-Pérez,
  • Agustín Olmedo-Juárez and ,
  • Roberto González-Garduño,
  • José Luis Ponce-Covarrubias,
  • Nallely Rivero-Perez,
  • Felipe Patricio,
  • Huitziméngari Campos-García and
  • Samuel Ortega-Vargas
  • + 7 authors+ 2 authors

Antimicrobial resistance threatens human and animal health, while the development of new antibacterial agents remains slow. Phage display can identify peptides and antibody fragments for bacterial targeting, antimicrobial engineering, antibiotic pote...

(This article belongs to the Special Issue Feature Reviews for Applied Biosciences)
  • Article
  • Open Access
196 Views
24 Pages

Are Essential Oils Effective Against Human Skin Yeasts? Experimental Evidence from In Vitro Assays

  • Ana I. Lopes,
  • Cláudia S. Oliveira,
  • Manuela E. Pintado and
  • Freni K. Tavaria

Skin-associated yeasts are involved in several skin disorders, while increasing resistance to conventional antifungal agents highlights the need for alternative therapeutic approaches. Essential oils (EOs) have attracted increasing scientific interes...

(This article belongs to the Special Issue Plant Natural Compounds: From Discovery to Application (2nd Edition))
  • Article
  • Open Access
141 Views
29 Pages

Endophytic Fungi from Emblica urinaria as Emerging Sources to Target Multidrug-Resistant Bacterial and Fungal Pathogens

  • Bac V. G. Nguyen,
  • Linh X. T. Tran,
  • Giang Ngoc Linh Vo,
  • Quan Minh Hoang and ,
  • Thu Q. H. Le,
  • Phong Gia Vu,
  • Minh-Tri Le,
  • Anh-Tu Nguyen Ha and
  • Phuoc-Vinh Nguyen
  • + 4 authors+ 0 author

Antimicrobial resistance has become a major global health concern. In this context, the present study aimed to explore endophytic microorganisms from Emblica urinaria as a potential source of antimicrobial metabolites. E. urinaria samples were collec...

  • Review
  • Open Access
178 Views
42 Pages

Neurotransmitter Systems and Systemic Resilience in the Healthy Aging Brain

  • María Jesús Ramírez-Expósito,
  • José Manuel Martínez-Martos and
  • Cristina Cueto-Ureña

Physiological brain aging is driven by progressive neuro-glial-vascular remodeling, in which neurotransmitter systems serve as the core functional network integrating central resilience and peripheral systemic signaling. This narrative review provide...

(This article belongs to the Special Issue Molecular Neurobiology Across the Lifespan: Development, Metabolism, Oxidative Stress and Disease)
  • Article
  • Open Access
308 Views
27 Pages

In Silico Systems-Level Analysis of Extracellular Vesicle-Associated miRNAs Potentially Involved in Bone Regeneration

  • Patrícia Sesterheim,
  • Fernando da Silva Martins,
  • Fernanda Marques da Silva and
  • Eduardo Rolim Teixeira

Background: Extracellular vesicle (EV)-associated microRNAs (miRNAs) have been implicated in intercellular signaling processes relevant to bone regeneration; however, the regulatory relationships through which selected EV-associated miRNAs may conver...

  • Article
  • Open Access
228 Views
19 Pages

Transcriptome-Wide Characterization of the PPR Gene Family Reveals Stable Plastid-Targeted PPR Expression Alongside Reduced Photosystem II Performance in HLB-Affected Persian Lime

  • Ricardo Santillán-Mendoza,
  • Nelly A. González-Oviedo,
  • Mauricio Andrade-Marcial,
  • Berenice Preza-Murrieta and ,
  • Humberto J. Estrella-Maldonado and
  • Felipe R. Flores-de la Rosa
  • + 1 author+ 0 author

Huanglongbing (HLB), caused by Candidatus Liberibacter asiaticus (CLas), severely impairs photosynthesis and chloroplast function in citrus. However, Persian lime (Citrus latifolia) exhibits remarkable tolerance, maintaining productivity despite infe...

  • Conference Report
  • Open Access
256 Views
180 Pages

The 11th International Conference on Plasma Medicine (ICPM11), held jointly with the 10th International Workshop on Plasmas for Cancer Treatment (IWPCT10), took place from 31 May to 5 June 2026 in Gifu, Japan. The meeting brought together 225 scienti...

  • Article
  • Open Access
200 Views
19 Pages

The poor regenerative capacity of articular cartilage and the limited efficacy of current treatments in restoring its extracellular matrix contribute to osteoarthritis. This study investigated an emerging approach using the secretome of mesenchymal s...

(This article belongs to the Special Issue Anatomy and Regenerative Medicine: From Methods to Applications (3rd Edition))
  • Article
  • Open Access
245 Views
23 Pages

Molecular-Weight-Dependent Effects of Fish-Derived Peptide Biostimulants on Carbon–Nitrogen Metabolism and Anthocyanin Biosynthesis in Purple Lettuce

  • Norma Elvira Muguruza Crispin,
  • Carmen del Pilar Alvarez Quinteros,
  • Genaro Christian Pesantes Arriola,
  • Elvira Teófila Castañeda Chirre and ,
  • Fiorella Victoria Luperdi Rios,
  • Jacqueline Roxana Reaño Rivera,
  • Marleni Vilma Bautista Espinoza,
  • Franklin O. Areche,
  • Olivia Magaly Luque Vilca and
  • Geraldina Rebeca Parihuaman Quinde
  • + 10 authors+ 5 authors

Fish-processing by-products represent a potential source of peptide-based plant biostimulants, but the relationships between peptide molecular characteristics and plant metabolic responses remain poorly defined. This study evaluated the effects of pe...

(This article belongs to the Special Issue Plant Natural Compounds: From Discovery to Application (2nd Edition))
  • Article
  • Open Access
259 Views
16 Pages

Authentication of commercial oregano products remains challenging because processing may hinder the identification of undeclared botanical species. In this study, a stepwise molecular approach integrating DNA mini-barcoding, species-specific real-tim...

  • Review
  • Open Access
474 Views
33 Pages

The increasing incidence of fungal infections caused by Candida albicans and the multidrug-resistant emerging pathogen Candidozyma auris (formerly Candida auris) represents a major global healthcare challenge. Current antifungal therapies are limited...

  • Systematic Review
  • Open Access
270 Views
26 Pages

Single-pulse transcranial magnetic stimulation (spTMS) is widely used to investigate human corticospinal and cortical physiology, yet individual physiological measures provide only partial information about cortical function. This systematic review a...

  • Review
  • Open Access
331 Views
45 Pages

Gut Microbiome and Cognitive Frailty in Older Adults: Human Evidence, Biomarker Validity, and Prospects for Clinical Translation

  • Cătălina Raluca Nuță,
  • Lidia Băjenaru,
  • Ana Maria Moiceanu Sovarel and
  • Ovidiu Lucian Băjenaru

Cognitive frailty combines physical frailty with cognitive impairment without dementia, identifying older adults vulnerable to adverse outcomes. Candidate microbiome-related pathways in cognitive frailty include dysfunction of the intestinal epitheli...

  • Article
  • Open Access
293 Views
17 Pages

Green and Sustainable Production of Bioactive Protein Hydrolysates from Hilsa (Tenualosa ilisha) Using Ultrasound-Assisted Subcritical Water Hydrolysis

  • Monjurul Haq,
  • Sharmin Suraiya,
  • Raju Ahmed,
  • Md. Tanvir Ahmed and ,
  • Md. Sadek Ali,
  • Vikash Chandra Roy,
  • Jin-Seok Park,
  • Hee-Jeong Lee,
  • Truc Cong Ho and
  • Byung-Soo Chun
  • + 7 authors+ 2 authors

Hilsa muscle biomass was hydrolyzed to recover bioactive proteins and peptides using subcritical water hydrolysis (SWH) with ultrasound pretreatment at various temperatures (150–250 °C) and pressures (40–80 bar). Hydrolysis yield was...

(This article belongs to the Special Issue Functional Food: Molecular Nutrition, Emerging Technologies and Applications)
  • Article
  • Open Access
508 Views
21 Pages

Water contamination by heavy metals is a major global concern resulting from anthropogenic activities and natural geological processes. Their removal and recovery are important for protecting water resources and addressing the depletion of critical m...

(This article belongs to the Topic Biomass Use and its Health and Environmental Effects)
  • Article
  • Open Access
402 Views
17 Pages

This study aimed to optimize pigment production from Monascus purpureus TISTR3090 using Khao Klong broken rice (brown rice) as a solid substrate and to evaluate the antioxidant and anti-aging activities of the resulting pigment extracts. Solid-state...

  • Article
  • Open Access
251 Views
20 Pages

Production and Characterization of a Bioactive Rich Extract from the Macroalga Pyropia orbicularis for the Development of Novel Functional Ingredients

  • Carla P. Morales-Guzmán,
  • Inés A. Cea-Pavez,
  • Inmaculada Moscoso-Ruiz,
  • David Manteca-Bautista and ,
  • Vito Verardo and
  • Martin Gotteland
  • + 1 author+ 0 author

Macroalgae are a valuable source of bioactive substances, particularly phenolic compounds with antioxidant properties. However, conventional extraction methods frequently rely on non-GRAS solvents, limiting their applicability in food and nutraceutic...

(This article belongs to the Special Issue Functional Food: Molecular Nutrition, Emerging Technologies and Applications)
  • Article
  • Open Access
342 Views
18 Pages

Adipose-Targeted Gene Regulation Using Synthetic Promoters and a Light-Switchable Transcription System

  • Stanislav Boychenko,
  • Anastasia Dakhnevich,
  • Tatiana Semerikova and
  • Alexander D. Egorov

Obesity is a global health threat, which continues to rise in prevalence worldwide, driving increases in related chronic diseases such as type 2 diabetes and cardiovascular conditions. While the sheer scale of the crisis affects over a billion people...

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Appl. Biosci. - ISSN 2813-0464