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Separations, Volume 10, Issue 8

August 2023 - 42 articles

Cover Story: The increasing importance of therapeutic antibodies in the treatment of cancer, autoimmune, and infectious diseases has led to a growing demand for efficient purification strategies in the manufacturing process. Efficient processes are characterized by high quality, low cost, and environmental friendliness. Miniaturization and automation capabilities have the potential to improve the efficiency of chromatographic processes. In this study, two miniaturized chromatographic formats are used to purify an IgG antibody from cell cultures. The automation of miniaturized chromatographic purification is realized with a liquid handling workstation. The system and the purification results are compared in terms of quality, cost, and sustainability, showing high yields and a significant reduction in laboratory waste. View this paper
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Articles (42)

  • Feature Paper
  • Article
  • Open Access
2 Citations
1,879 Views
11 Pages

Carnosinase (i.e., CN1; E.C. 3.4.13.20) is an enzyme found in the sera of higher primates. CN1 preferentially catalyzes the hydrolysis of natural, orally adsorbed histidine dipeptides like carnosine (i.e., β-alanyl-L-histidine). This is the reas...

  • Article
  • Open Access
7 Citations
3,633 Views
16 Pages

The present study employed a modified QuEChERS method to systematically analyze the presence of fifteen quinolone and seven tetracycline antibiotic residues in local animal food. Additionally, a multi-level four-factor Box–Behnken design (BBD)...

  • Article
  • Open Access
6 Citations
1,969 Views
15 Pages

Impact of Cumin (Cuminum cyminum) Incorporation on the Generation of Heterocyclic Aromatic Amines in Meatballs

  • Elif Ekiz,
  • Adem Savaş,
  • Eyad Aoudeh,
  • Zeynep Elbir,
  • Emel Oz,
  • Charalampos Proestos,
  • Naushad Ahmad and
  • Fatih Oz

In the current study, the impacts of using cumin (0.5% and 1%, w/w) in beef meatballs on heterocyclic aromatic amines (HAAs) formation and some quality characteristics when cooked at 150 and 250 °C were investigated. It was found that using of cu...

  • Article
  • Open Access
7 Citations
3,002 Views
15 Pages

UHPLC-ToF-MS as a High-Resolution Mass Spectrometry Tool for Veterinary Drug Quantification in Milk

  • Marta Leite,
  • Ana Rita Marques,
  • Ana Sofia Vila Pouca,
  • Silvia Cruz Barros,
  • Jorge Barbosa,
  • Fernando Ramos,
  • Isabel Maria Afonso and
  • Andreia Freitas

Milk is one of the most widely consumed foods in the world, despite the increasing consumption of plant-based alternatives. Although rich in nutrients and believed by consumers to be free of undesirable contaminants, milk, whether of animal or plant...

  • Feature Paper
  • Article
  • Open Access
6 Citations
4,676 Views
11 Pages

Determining the Hydrophobicity Index of Protected Amino Acids and Common Protecting Groups

  • Varshitha Gavva,
  • Othman Al Musaimi,
  • Colin Bent and
  • Daryl R. Williams

Peptides are in great demand in the pharmaceutical arena and a majority of these peptides contain 20 or more amino acids. They are infrequently synthesised using the fragment condensation approach. A key limitation in adopting this approach more comm...

  • Article
  • Open Access
3 Citations
9,335 Views
14 Pages

Evaluation of BPA and Bis-GMA Release from Recent Dental Composite Materials by LC-MS/MS

  • Lígia Lopes-Rocha,
  • Virgínia M. F. Gonçalves,
  • Sara C. Cunha,
  • José O. Fernandes,
  • Teresa Pinho and
  • Maria Elizabeth Tiritan

Bisphenol-A (BPA) is a xenoestrogen widely used as a synthetic precursor of resin monomers. There is arise need to acquire BPA-free resin-matrix composites to prevent the health effects of BPA. Six composites with distinctive manufacturer specificati...

  • Article
  • Open Access
5 Citations
2,080 Views
17 Pages

Molecularly Imprinted Polymer-Based Nanoporous Carbon Nanocomposite for Effective Adsorption of Hg(II) Ions from Aqueous Suspensions

  • Lawal Abubakar,
  • Nor Azah Yusof,
  • Abdul Halim Abdullah,
  • Faruq Mohammad,
  • Mohd Hanif Wahid,
  • Suhainie Ismail,
  • Zulaiha Abdul Rahim,
  • Hamad A. Al-Lohedan and
  • Ahmed A. Soleiman

Due to the release of hazardous heavy metals from various industries, water pollution has become one of the biggest challenges for environmental scientists today. Mercury Hg(II) is regarded as one of the most toxic heavy metals due to its ability to...

  • Review
  • Open Access
9 Citations
8,985 Views
33 Pages

MALDI-TOF MS: A Promising Analytical Approach to Cancer Diagnostics and Monitoring

  • Patrícia Sousa,
  • Laurentina Silva,
  • Catarina Luís,
  • José S. Câmara and
  • Rosa Perestrelo

Cancer remains the second most common cause of death after cardiovascular diseases, accounting for nearly 10 million deaths in 2020. Although the incidence of cancer increases considerably with age, the cancer burden can also be reduced and have a hi...

  • Feature Paper
  • Article
  • Open Access
7 Citations
2,901 Views
17 Pages

Solid–Liquid Extraction of Bioactive Molecules from White Grape Skin: Optimization and Near-Infrared Spectroscopy

  • Tea Sokač Cvetnić,
  • Korina Krog,
  • Maja Benković,
  • Tamara Jurina,
  • Davor Valinger,
  • Jasenka Gajdoš Kljusurić,
  • Ivana Radojčić Redovniković and
  • Ana Jurinjak Tušek

In this work, the solid–liquid extraction of bioactive molecules from grape skin was performed using water as the extraction solvent. The effects of extraction time (t = 60, 75, and 90 min), extraction temperature (T = 40, 60, and 80 °C), solid–liqui...

  • Article
  • Open Access
2 Citations
2,043 Views
12 Pages

Study on Treatment Performance of Desulfurization Wastewater by Zero-Valent Iron Fenton-like Process

  • Ziguo Liu,
  • Wei Zhou,
  • Xianli Liu,
  • Xuefen Yang,
  • Wei Yang and
  • Han Zheng

In this study, the zero-valent iron Fenton reagent (ZVI Fenton-like) system was combined with the chemical precipitation method for the deep treatment of desulfurization wastewater from coal-fired power plants, and the chemical oxygen demand (COD) wa...

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Separations - ISSN 2297-8739