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Separations, Volume 10, Issue 8

2023 August - 42 articles

Cover Story: The increasing importance of therapeutic antibodies in the treatment of cancer, autoimmune, and infectious diseases has led to a growing demand for efficient purification strategies in the manufacturing process. Efficient processes are characterized by high quality, low cost, and environmental friendliness. Miniaturization and automation capabilities have the potential to improve the efficiency of chromatographic processes. In this study, two miniaturized chromatographic formats are used to purify an IgG antibody from cell cultures. The automation of miniaturized chromatographic purification is realized with a liquid handling workstation. The system and the purification results are compared in terms of quality, cost, and sustainability, showing high yields and a significant reduction in laboratory waste. View this paper
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Articles (42)

  • Feature Paper
  • Article
  • Open Access
2 Citations
1,953 Views
11 Pages

Carnosinase (i.e., CN1; E.C. 3.4.13.20) is an enzyme found in the sera of higher primates. CN1 preferentially catalyzes the hydrolysis of natural, orally adsorbed histidine dipeptides like carnosine (i.e., β-alanyl-L-histidine). This is the reas...

  • Article
  • Open Access
7 Citations
3,819 Views
16 Pages

The present study employed a modified QuEChERS method to systematically analyze the presence of fifteen quinolone and seven tetracycline antibiotic residues in local animal food. Additionally, a multi-level four-factor Box–Behnken design (BBD)...

  • Article
  • Open Access
6 Citations
2,046 Views
15 Pages

Impact of Cumin (Cuminum cyminum) Incorporation on the Generation of Heterocyclic Aromatic Amines in Meatballs

  • Elif Ekiz,
  • Adem Savaş,
  • Eyad Aoudeh,
  • Zeynep Elbir,
  • Emel Oz,
  • Charalampos Proestos,
  • Naushad Ahmad and
  • Fatih Oz

In the current study, the impacts of using cumin (0.5% and 1%, w/w) in beef meatballs on heterocyclic aromatic amines (HAAs) formation and some quality characteristics when cooked at 150 and 250 °C were investigated. It was found that using of cu...

  • Article
  • Open Access
8 Citations
3,112 Views
15 Pages

UHPLC-ToF-MS as a High-Resolution Mass Spectrometry Tool for Veterinary Drug Quantification in Milk

  • Marta Leite,
  • Ana Rita Marques,
  • Ana Sofia Vila Pouca,
  • Silvia Cruz Barros,
  • Jorge Barbosa,
  • Fernando Ramos,
  • Isabel Maria Afonso and
  • Andreia Freitas

Milk is one of the most widely consumed foods in the world, despite the increasing consumption of plant-based alternatives. Although rich in nutrients and believed by consumers to be free of undesirable contaminants, milk, whether of animal or plant...

  • Feature Paper
  • Article
  • Open Access
6 Citations
4,902 Views
11 Pages

Determining the Hydrophobicity Index of Protected Amino Acids and Common Protecting Groups

  • Varshitha Gavva,
  • Othman Al Musaimi,
  • Colin Bent and
  • Daryl R. Williams

Peptides are in great demand in the pharmaceutical arena and a majority of these peptides contain 20 or more amino acids. They are infrequently synthesised using the fragment condensation approach. A key limitation in adopting this approach more comm...

  • Article
  • Open Access
5 Citations
9,730 Views
14 Pages

Evaluation of BPA and Bis-GMA Release from Recent Dental Composite Materials by LC-MS/MS

  • Lígia Lopes-Rocha,
  • Virgínia M. F. Gonçalves,
  • Sara C. Cunha,
  • José O. Fernandes,
  • Teresa Pinho and
  • Maria Elizabeth Tiritan

Bisphenol-A (BPA) is a xenoestrogen widely used as a synthetic precursor of resin monomers. There is arise need to acquire BPA-free resin-matrix composites to prevent the health effects of BPA. Six composites with distinctive manufacturer specificati...

  • Article
  • Open Access
5 Citations
2,156 Views
17 Pages

Molecularly Imprinted Polymer-Based Nanoporous Carbon Nanocomposite for Effective Adsorption of Hg(II) Ions from Aqueous Suspensions

  • Lawal Abubakar,
  • Nor Azah Yusof,
  • Abdul Halim Abdullah,
  • Faruq Mohammad,
  • Mohd Hanif Wahid,
  • Suhainie Ismail,
  • Zulaiha Abdul Rahim,
  • Hamad A. Al-Lohedan and
  • Ahmed A. Soleiman

Due to the release of hazardous heavy metals from various industries, water pollution has become one of the biggest challenges for environmental scientists today. Mercury Hg(II) is regarded as one of the most toxic heavy metals due to its ability to...

  • Review
  • Open Access
11 Citations
9,371 Views
33 Pages

MALDI-TOF MS: A Promising Analytical Approach to Cancer Diagnostics and Monitoring

  • Patrícia Sousa,
  • Laurentina Silva,
  • Catarina Luís,
  • José S. Câmara and
  • Rosa Perestrelo

Cancer remains the second most common cause of death after cardiovascular diseases, accounting for nearly 10 million deaths in 2020. Although the incidence of cancer increases considerably with age, the cancer burden can also be reduced and have a hi...

  • Feature Paper
  • Article
  • Open Access
8 Citations
3,057 Views
17 Pages

Solid–Liquid Extraction of Bioactive Molecules from White Grape Skin: Optimization and Near-Infrared Spectroscopy

  • Tea Sokač Cvetnić,
  • Korina Krog,
  • Maja Benković,
  • Tamara Jurina,
  • Davor Valinger,
  • Jasenka Gajdoš Kljusurić,
  • Ivana Radojčić Redovniković and
  • Ana Jurinjak Tušek

In this work, the solid–liquid extraction of bioactive molecules from grape skin was performed using water as the extraction solvent. The effects of extraction time (t = 60, 75, and 90 min), extraction temperature (T = 40, 60, and 80 °C), solid–liqui...

  • Article
  • Open Access
2 Citations
2,144 Views
12 Pages

Study on Treatment Performance of Desulfurization Wastewater by Zero-Valent Iron Fenton-like Process

  • Ziguo Liu,
  • Wei Zhou,
  • Xianli Liu,
  • Xuefen Yang,
  • Wei Yang and
  • Han Zheng

In this study, the zero-valent iron Fenton reagent (ZVI Fenton-like) system was combined with the chemical precipitation method for the deep treatment of desulfurization wastewater from coal-fired power plants, and the chemical oxygen demand (COD) wa...

  • Article
  • Open Access
1 Citations
2,222 Views
17 Pages

Savolitinib (Orpathys®), was developed by (HUTCHMED (Shanghai, China) and, AstraZeneca (Gaithersburg, Maryland, USA), is an inhibitor of the c-Met receptor tyrosine kinase that is orally bioavailable. It was designed for the treatment of pillary...

  • Article
  • Open Access
10 Citations
2,536 Views
15 Pages

Analytical and Preparative Separation of Softwood Lignans by Supercritical Fluid Chromatography

  • Nikolay V. Ul’yanovskii,
  • Aleksandra A. Onuchina,
  • Denis V. Ovchinnikov,
  • Anna V. Faleva,
  • Natalia S. Gorbova and
  • Dmitry S. Kosyakov

Lignans are widespread polyphenolic secondary plant metabolites possessing high biological activity. One of the most promising industrial-scale sources of such compounds is coniferous knotwood, containing a large number of polyphenolic compounds. The...

  • Article
  • Open Access
2 Citations
2,634 Views
16 Pages

Expression, Purification and Characterization of a Novel Rusticyanin from the Psychrotolerant Acidithiobacillus ferrivorans

  • Yuandong Liu,
  • Lixiang Chen,
  • Xiangdong Shangguan,
  • Jingying Ouyang,
  • Jiayu He,
  • Kan Wang,
  • Yan Tong,
  • Runlan Yu,
  • Weimin Zeng and
  • Guanzhou Qiu
  • + 2 authors

Rusticyanin plays a crucial role in ferrous oxidation of sulfide minerals during bioleaching for industrial metal extraction. Diverse isoforms of rusticyanin have been found, but until now, except for type-A rusticyanin, other isoforms or sources of...

  • Article
  • Open Access
2 Citations
3,492 Views
15 Pages

Therapeutic antibodies are an area of major interest in treating infectious diseases, autoimmune diseases, and cancer. Efficient purification strategies are required to discover and produce antibodies, representing a significant cost driver. Miniatur...

  • Article
  • Open Access
2 Citations
1,686 Views
17 Pages

Investigation of the change rate for contaminant parameters is important to characterize dense non-aqueous phase liquid (DNAPL) transport and distribution in groundwater systems. In this study, four experiments of perchloroethylene (PCE) migration ar...

  • Article
  • Open Access
5 Citations
4,225 Views
23 Pages

High-Degree Oxidative Desulfurization of a Commercial Marine Fuel Using Deep Eutectic Solvents and Their Recycling Process

  • Olga Thoda,
  • Efstratios Svinterikos,
  • Konstantinos Miltiadis Sakkas,
  • Anastasia Maria Moschovi and
  • Iakovos Yakoumis

Escalating environmental concerns have dictated the need to develop innovative methods for efficiently desulfurizing marine fuels (heavy fuel oils). In this work, the oxidative desulfurization method using deep eutectic solvents (DESs) was applied to...

  • Article
  • Open Access
5 Citations
4,054 Views
18 Pages

The Development and Validation of a Targeted LC-HRAM-MS/MS Methodology to Separate and Quantify p-Synephrine and m-Synephrine in Dietary Supplements and Herbal Preparations

  • Celine Vanhee,
  • Sophia Barhdadi,
  • Angélique Kamugisha,
  • Tanika Van Mulders,
  • Kevin Vanbrusselen,
  • Marie Willocx and
  • Eric Deconinck

Dietary supplements containing Citrus aurantium or p-synephrine remain very popular in Europe and the United States of America (USA). They are primarily sold as weight loss enhancers, although their efficacy and the safety are still under scrutiny. T...

  • Article
  • Open Access
2,478 Views
13 Pages

The primary objective of this study was to pragmatically implement an extraction process using slug flow, wherein two immiscible phases, aqueous and oil, are alternatively channeled through a conduit to encourage mass exchange across the interface. T...

  • Article
  • Open Access
3 Citations
3,272 Views
18 Pages

Effects of Catha edulis (Khat) on the Pharmacokinetics of Metformin in Diabetic Rats Using UPLC/MS/MS Analysis and Its Impact on Hepatic CYP450 Enzymes

  • Ali S. Alqahtani,
  • Mohammad Khalid Parvez,
  • Abdulaziz M. Alqahtani,
  • Omer I. Fantoukh,
  • Rashed N. Herqash,
  • Ehab M. Elzayat,
  • Fahd A. Nasr,
  • Essam Ezzeldin,
  • Mousallam M. Almousallam and
  • Mohammad Raish

Catha edulis Forsk., commonly known as Khat, is a stimulant plant that is chewed in the Horn of Africa and the southern regions of the Arabian Peninsula. It is often used alongside conventional drugs, but there is a lack of research on its interactio...

  • Review
  • Open Access
14 Citations
3,369 Views
20 Pages

Heavy metals in water pose a great threat to human health, and water treatment remains a critical issue globally. Among various water treatment techniques, adsorption has been proven to be an excellent way to remove heavy metals from water due to its...

  • Article
  • Open Access
1 Citations
2,411 Views
13 Pages

Enhancement of Photocatalytic Rhodamine B Degradation over Magnesium–Manganese Baring Extracted Iron Oxalate from Converter Slag

  • Chitiphon Chuaicham,
  • Jirawat Trakulmututa,
  • Sulakshana Shenoy,
  • Vellaichamy Balakumar,
  • Phatchada Santawaja,
  • Shinji Kudo,
  • Karthikeyan Sekar and
  • Keiko Sasaki

In this work, iron oxalate from converter slag (FeOX-Slag) was produced by extraction of iron from converter slag using oxalic acid, followed by photo-reduction. The FeOX-Slag sample was subjected to various characterization techniques, including X-r...

  • Article
  • Open Access
29 Citations
4,175 Views
17 Pages

Removal of Pb(II) from Aqueous Solution and Adsorption Kinetics of Corn Stalk Biochar

  • Wenling Yang,
  • Chaoyang Lu,
  • Bo Liang,
  • Chaohui Yin,
  • Gao Lei,
  • Baitao Wang,
  • Xiaokai Zhou,
  • Jing Zhen,
  • Shujing Quan and
  • Yanyan Jing

In this work, the Pb adsorption and removal ability of biochar from simulated Pb(II)-contaminated wastewater, adsorption isotherms, kinetics, and thermodynamics were studied. Adsorption characteristics of biochar on Pb(II) were analyzed by Fourier tr...

  • Article
  • Open Access
2 Citations
1,922 Views
11 Pages

Optimization of the Preparation of Hydrophilic Poly(DHPMA-co-MBA) Monolithic Capillary Columns: A New Support for Affinity Chromatography

  • Julie Gil,
  • Gaëtan Passalacqua,
  • Adrien Deloche,
  • François-Xavier Vidal,
  • Vincent Dugas and
  • Claire Demesmay

In miniaturized affinity chromatography, the development of hydrophilic organic monoliths with reduced non-specific interactions and high-protein grafting capacity remains a hot topic. In this work, we propose the one-step synthesis of a diol organic...

  • Article
  • Open Access
3 Citations
1,989 Views
13 Pages

Chemometric Approach of Different Extraction Conditions on Scavenging Activity of Helichrisym italicum (Roth) G. Don Extracts

  • Branislava Teofilović,
  • Filip Balaž,
  • Milica Karadžić Banjac,
  • Nevena Grujić-Letić,
  • Emilia Gligorić,
  • Strahinja Kovačević,
  • Sanja Podunavac-Kuzmanović and
  • Srđan Stojanović

Helichrysum italicum (Roth) G. Don is widely used as a liver stimulant and diuretic, as well as an antioxidant, antiallergenic, antimicrobial, antiviral, choleric, and antihypertensive agent. Therefore, the purpose of this study was to analyze the di...

  • Article
  • Open Access
10 Citations
4,099 Views
13 Pages

Screening and Experimental Validation for Selection of Open Metal Sites Metal-Organic Framework (M-CPO-27, M = Co, Mg, Ni and Zn) to Capture CO2

  • Nor Ernie Fatriyah Kari,
  • Marhaina Ismail,
  • Aqeel Ahmad,
  • Khaliesah Kamal,
  • Thiam Leng Chew and
  • Mohamad Azmi Bustam

The release of CO2 into the atmosphere has become a primary issue nowadays. Recently, researchers found Metal-Organic Frameworks M-CPO-27 (M = Mg, Co, Ni, and Zn) to be revolutionary for CO2 adsorption due to the presence of open metal sites enhancin...

  • Article
  • Open Access
1 Citations
2,039 Views
13 Pages

Isolation of Osteoblastic Differentiation-Inducing Constituents from Peanut Sprouts and Development of Optimal Extraction Method

  • Eun-Sang Cho,
  • Chung Hyeon Lee,
  • Min Sung Ko,
  • Jee Yeon Choi,
  • Kwang Woo Hwang and
  • So-Young Park

Osteoporosis, one of the most common bone diseases, results from an imbalance between bone formation and resorption. Osteoblasts are primarily involved in bone formation, whereas osteoclasts are involved in bone resorption. Therefore, any substances...

  • Article
  • Open Access
4 Citations
2,755 Views
18 Pages

Water is one of the greenest and most accessible solvents. To harness its potential, the water-based microwave-assisted extraction (MAE) of pigments from madder was optimized by the response surface methodology (RSM) using a Box–Behnken experim...

  • Article
  • Open Access
2,218 Views
16 Pages

Study of Estimated Ultimate Recovery Prediction and Multi-Stage Supercharging Technology for Shale Gas Wells

  • Yanli Luo,
  • Jianying Yang,
  • Man Chen,
  • Liu Yang,
  • Hao Peng,
  • Jinyuan Liang and
  • Liming Zhang

The development of shale gas reservoirs often involves the utilization of horizontal well segmental multi-stage fracturing techniques. However, these reservoirs face challenges, such as rapid initial wellhead pressure and production decline, leading...

  • Article
  • Open Access
1 Citations
2,186 Views
10 Pages

Separation of (2E,4E)-deca-2,4-dienal from a Biocatalytic Reaction Mixture Using Hydrophobic Adsorbents

  • Marta Ostrihoňová,
  • Monika Antošová,
  • Hana Dobiašová,
  • Justína Čuchorová,
  • Kvetoslava Vranková and
  • Milan Polakovič

Biocatalytic oxidation of a fatty acid preparation by enzymes released from a plant extract provided (2E,4E)-deca-2,4-dienal (2,4-DDAL), a valuable natural aroma. The post-reaction mixture contained several other compounds, from which 2,4-DDAL must b...

  • Article
  • Open Access
7 Citations
2,023 Views
12 Pages

Hexavalent chromium (Cr(VI)) is one of the most toxic heavy metals found in industrial wastewater, so many researchers are working to develop efficient and environmentally friendly removal methods. It has been reported that natural biomass and its de...

  • Communication
  • Open Access
1 Citations
1,852 Views
10 Pages

Sedum kamtschaticum Fischer, of the Crassulaceae family, is a perennial and medicinal plant used in Asian folk medicine to alleviate inflammatory disease and improve blood circulation. As part of our ongoing exploration into natural products, seeking...

  • Article
  • Open Access
2 Citations
2,047 Views
15 Pages

A simple, effective, and highly sensitive analytical approach was created and applied in this study for the accurate measurement of three β2-agonist residues (clenbuterol, salbutamol, and ractopamine) in meat samples. In the course of the experi...

  • Article
  • Open Access
1 Citations
5,767 Views
24 Pages

Biosynthesis of Salbutamol-4′-O-sulfate as Reference for Identification of Intake Routes and Enantiopure Salbutamol Administration by Achiral UHPLC-MS/MS

  • Annika Lisa Jendretzki,
  • Lukas Corbinian Harps,
  • Yanan Sun,
  • Felix Bredendiek,
  • Matthias Bureik,
  • Ulrich Girreser,
  • Xavier de la Torre,
  • Francesco M. Botrè and
  • Maria Kristina Parr

The aim of the study was a comprehensive and quantitative determination of salbutamol and its sulfoconjugated major metabolite in urine samples using achiral ultrahigh performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). Therefor...

  • Article
  • Open Access
7 Citations
3,387 Views
18 Pages

The removal of the azo dyes Orange II (OII) and Reactive Black 5 (RB5) from aqueous solutions was studied using three types of adsorbents derived from chitosan: unmodified chitosan beads (un-Ch), chitosan beads tuned with a deep eutectic solvent (DES...

  • Article
  • Open Access
5 Citations
1,908 Views
13 Pages

Effects of Dissolved Organic Matter on the Release of Soluble Phosphorus and Fluoride Ion from Phosphate Ore

  • Fengjiao Zhang,
  • Huaying Liu,
  • Yanqiong Ma,
  • Yingjie Li,
  • Cheng Tie and
  • Qilin Zhao

Unreasonable storage of phosphate ore is becoming an important pathway causing phosphate pollution in the surrounding aquatic environment. However, there is little research on the influence of dissolved organic matter (DOM) in water on the fate of ph...

  • Review
  • Open Access
56 Citations
22,046 Views
30 Pages

The utilization of anion exchange membranes (AEMs) has revolutionized the field of electrochemical applications, particularly in water electrolysis and fuel cells. This review paper provides a comprehensive analysis of recent studies conducted on var...

  • Article
  • Open Access
2 Citations
3,422 Views
16 Pages

A Novel UHPLC-MS/MS-Based Bioanalytical Method Developed for S-Allyl Cysteine in the Establishment of a Comparative Pharmacokinetic Study

  • Mohd Faiyaz Khan,
  • Niyaz Ahmad,
  • Faisal K. Alkholifi,
  • Zabih Ullah,
  • Sadaf Farooqui,
  • Nazia Khan,
  • Mohammed Saifuddin Khalid,
  • Mir Naiman Ali and
  • Hajera Tabassum

A newly UHPLC-MS/MS method development and validation for S-Allyl Cysteine was used to evaluate the comparative pharmacokinetic parameters. SC PLGA NPs (S-Allyl Cysteine Poly (D,L-lactide-co-glycolic acid) Nanoparticles) were developed by the emulsio...

  • Article
  • Open Access
13 Citations
2,655 Views
19 Pages

The adsorption of phenol (Ph), 4-chlorophenol (CP), and 4-cresol (MP) from aqueous solutions on three carbonaceous materials of diverse origins but similar specific surface areas was investigated. Vulcan XC72 carbon black (CB), AKP-5 activated coke (...

  • Article
  • Open Access
18 Citations
3,973 Views
19 Pages

Bioactive compounds from plants play an important role in slowing many neurodegenerative diseases, such as Alzheimer’s disease, by inhibiting cholinesterase enzymes. Studies have shown that oxidative stress is associated with the development of...

  • Article
  • Open Access
6 Citations
3,818 Views
12 Pages

GC-MS Analysis and Bioactivity Screening of Leaves and Fruits of Zanthoxylum armatum DC.

  • Jie Ma,
  • Liping Ning,
  • Jingyan Wang,
  • Wei Gong,
  • Yue Gao and
  • Mei Li

Zanthoxylum armatum DC. is a plant that has been homologated for medicine and food by the Chinese for three thousand years. In this study, the essential oils of fresh leaves and fruits were extracted by hydrodistillation, the aromas of fresh leaves a...

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Separations - ISSN 2297-8739