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Insects, Volume 16, Issue 6

2025 June - 98 articles

Cover Story: Drosophila subobscura is a species that stands out for its large chromosomal inversion polymorphism that affects its five long chromosomes. Investigations into natural populations have revealed that its inversion polymorphism has adaptive properties. In this work, we localize the breakpoints of inversions U6 and U8 as a first approach to candidate genes in the inversions that may be affected by selection. Interestingly, our results suggest that the origin of both inversions could have been mediated by transposons, and, in the case of U8, we found a new fold-back-type element characteristic of the subobscura species group that we have designated Ziga-ZagaView this paper
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Articles (98)

  • Review
  • Open Access
2 Citations
2,539 Views
20 Pages

Managing African Armyworm Outbreaks in Sub-Saharan Africa: Current Strategies and Future Directions

  • Grace Kinyanjui,
  • Kahsay Tadesse Mawcha,
  • Lawrence Nkosikhona Malinga,
  • Kaitlyn Soobramoney,
  • Phophi Ṋethononda,
  • Yoseph Assefa,
  • Chibuzor Onyinye Okonkwo and
  • Dennis Ndolo

19 June 2025

The African armyworm Spodoptera exempta (Lepidoptera: Noctuidae) is a significant pest that affects cereal crops and pasture grasses in sub-Saharan Africa. This migratory pest causes extensive defoliation, which can result in significant yield losses...

  • Article
  • Open Access
1 Citations
1,464 Views
20 Pages

Cell Structure of the Preoral Mycangia of Xyleborus (Coleoptera: Curculiondiae) Ambrosia Beetles

  • Ross A. Joseph,
  • Esther Tirmizi,
  • Abolfazl Masoudi and
  • Nemat O. Keyhani

19 June 2025

Ambrosia beetles have evolved specialized structures termed “mycangia”, which house and transport symbiotic microbes. Microbial partners include at least one obligate mutualistic filamentous fungus used as food for larvae and adults, and...

  • Article
  • Open Access
1 Citations
1,013 Views
14 Pages

19 June 2025

The effects of the gene encoding the ecdysone receptor (EcR) on the reproduction of the ladybug Coccinella septempunctata was evaluated. EcR transcription was measured by quantitative real-time PCR in ladybug adults reared on artificial diets with an...

  • Article
  • Open Access
1,210 Views
12 Pages

Nosemosis in Russian Apis mellifera L. Populations: Distribution and Association with Hybridization

  • Milyausha Kaskinova,
  • Luisa Gaifullina,
  • Gleb Zaitsev,
  • Alexandr Davydychev and
  • Elena Saltykova

18 June 2025

One of the common causes of mass death in bee colonies is the infectious disease nosemosis, which is caused by two types of microsporidia, Nosema apis and Nosema ceranae. Of the many factors contributing to the spread of nosemosis, in this paper we c...

  • Article
  • Open Access
1 Citations
3,670 Views
14 Pages

18 June 2025

Beetles of the family Mordellidae, important global pollinators, include Glipa, the third largest genus, which retains plesiomorphic traits related to pollination and is mainly found between 38° S–38° N. Existing studies on Glipa focus...

  • Article
  • Open Access
1,125 Views
22 Pages

18 June 2025

(1) Background: The larvae of Dioryctria sylvestrella typically bore into the shoots and cones of Pinus koraiensis, increasing tree breakage risk and reducing cone yield. (2) Methods: Five Beauveria bassiana strains were evaluated for virulence again...

  • Article
  • Open Access
1 Citations
1,530 Views
21 Pages

Trends and Emerging Hotspots in Toxicology of Chironomids: A Comprehensive Bibliometric Analysis

  • Wen-Bin Liu,
  • Wen-Xuan Pei,
  • Zi-Ming Shao,
  • Jia-Xin Nie,
  • Wei Cao and
  • Chun-Cai Yan

17 June 2025

(1) Background: Aquatic organisms are more sensitive to pollutants than terrestrial ones, making them ideal for ecotoxicology studies. Chironomids, a key bioindicator species, have been widely used in environmental pollution research. With the contin...

  • Article
  • Open Access
2 Citations
1,910 Views
29 Pages

17 June 2025

Olfaction has been extensively studied in the yellow fever mosquito, Aedes aegypti. This species uses its sense of smell to find blood hosts and other resources, contributing to its impact as a vector for human pathogens. Two major families of protei...

  • Article
  • Open Access
1,319 Views
27 Pages

17 June 2025

The exoskeleton of insects, known as the cuticle, necessitates regular renewal during molting and metamorphosis, with chitin being its primary structural component. Consequently, the molting and metamorphosis processes in insects are characterized by...

  • Article
  • Open Access
1,588 Views
15 Pages

Development of a New Trapping System with Potential Implementation as a Tool for Mosquito-Borne Arbovirus Surveillance

  • Luísa Maria Inácio da Silva,
  • Larissa Krokovsky,
  • Rafaela Cassiano Matos,
  • Gabriel da Luz Wallau and
  • Marcelo Henrique Santos Paiva

17 June 2025

Mosquitoes of the Aedes and Culex genera are primary vectors of arboviruses such as the dengue, Zika, chikungunya (CHIKV), Oropouche, and West Nile viruses, causing millions of infections annually. Standard virus detection in mosquitoes requires capt...

  • Article
  • Open Access
2 Citations
1,432 Views
13 Pages

Low-Temperature Phosphine Fumigation Is Effective Against Drosophila suzukii in Sweet Cherry

  • Hang Zou,
  • Li Li,
  • Jun Zhang,
  • Baishu Li,
  • Yu Xiao,
  • Yonglin Ren,
  • Ju Huang,
  • Wei Chen and
  • Tao Liu

17 June 2025

Drosophila suzukii, classified as a quarantine pest in some countries, poses a serious threat to global trade due to its ability to damage berries and cherries. Recent studies indicate that low-temperature phosphine (PH3) fumigation effectively contr...

  • Article
  • Open Access
988 Views
17 Pages

16 June 2025

The present investigation systematically elucidates the distinct functional specialization within the M1–M3 midgut sections of the significant agricultural pest, Nezara viridula. Employing an integrated transcriptomic analysis, three pivotal di...

  • Article
  • Open Access
1 Citations
1,003 Views
12 Pages

16 June 2025

Volatile cues are important in the host detection and discrimination of phytophagous insects, allowing them to find suitable hosts. Here, the electroantennography (EAG) and behavioral responses of female Thrips hawaiiensis to the floral volatiles of...

  • Article
  • Open Access
1,241 Views
20 Pages

Invertebrates of Siberia, a Potential Source of Animal Protein for Innovative Food and Feed Production: Biomass Nutrient Composition Change in the Earthworm Eisenia fetida (Savigny, 1826) and the House Cricket Acheta domesticus (Linnaeus, 1758)

  • Sergei E. Tshernyshev,
  • Andrei S. Babenko,
  • Irina B. Babkina,
  • Ruslan T.-O. Baghirov,
  • Vera P. Modyaeva,
  • Margarita D. Morozova,
  • Ksenia E. Skribtcova,
  • Elena Y. Subbotina,
  • Mikhail V. Shcherbakov and
  • Anastasia V. Simakova

16 June 2025

The possibility of designing the nutrient composition of invertebrate biomass was investigated. Two model species of terrestrial invertebrates, the house cricket Acheta domesticus (Linnaeus, 1758) and the earthworm Eisenia fetida (Savigny, 1826), wer...

  • Article
  • Open Access
1,023 Views
17 Pages

Time to Emergence of the Lyme Disease Pathogen in Habitats of the Northeastern U.S.A.

  • Dorothy Wallace,
  • Michael Palace,
  • Lucas Eli Price and
  • Xun Shi

16 June 2025

Ticks carry a range of pathogens, the best known of which causes Lyme disease, prevalent in the northeastern United States. Emerging diseases do not yet consist of a wide range of Lyme diseases, raising the question of how long it takes for a newly i...

  • Article
  • Open Access
977 Views
13 Pages

15 June 2025

This study takes the Jinfoping Small Watershed in the Loess Hilly Region as the research area. Through field investigation and laboratory analysis, combined with methods such as spatial autocorrelation analysis, the ordinary least squares method (OLS...

  • Article
  • Open Access
1 Citations
1,462 Views
15 Pages

15 June 2025

The bird cherry-oat aphid Rhopalosiphum padi (L.) poses a significant threat to wheat production, resulting in substantial yield reductions. Abamectin and acetamiprid are frequently utilized for management. This study assessed the sublethal effects o...

  • Article
  • Open Access
1 Citations
1,361 Views
17 Pages

13 June 2025

The flesh fly, Wohlfahrtia nuba (Wiedemann) (Diptera: Sarcophagidae), is one of the first necrophagous insects to arrive on a cadaver and is vital for understanding decomposition. Environmental factors, especially temperature, influence insect develo...

  • Article
  • Open Access
898 Views
14 Pages

13 June 2025

Histia rhodope is a defoliating pest that feeds mainly on the ornamental garden plant Bischofia polycarpa. Recently, frequent outbreaks of H. rhodope in Southern China have severely affected cityscapes and people’s lives. To provide a predictiv...

  • Article
  • Open Access
2 Citations
1,218 Views
14 Pages

Distribution Pattern and Change Prediction of Luprops orientalis (Coleoptera: Tenebrionidae) Suitable Area in East Asia Under Climate Change

  • Jieqiong Wang,
  • Shuangyi Wang,
  • Yunchun Li,
  • Shuangmei Ding,
  • Zhonghua Wei,
  • Aimin Shi and
  • Ding Yang

13 June 2025

Luprops orientalis (Motschulsky, 1868) is an economically important pest in traditional Chinese medicines, widely distributed in East Asia. However, the primary limiting factors affecting its distribution, potential suitable areas, as well as its res...

  • Article
  • Open Access
1,282 Views
24 Pages

Death-Leading Envenomization of Rabbits with Snake Versus Scorpion Venoms: A Comparative Forensic Investigation of Postmortem Decomposition and Beetle Succession

  • Afnan Saleh Al-Qurashi,
  • Mohammed Saleh Al-Khalifa,
  • Hathal Mohammed Al Dhafer,
  • Mahmoud Saleh Abdel-Dayem,
  • Hossam Ebaid and
  • Ashraf Mohamed Ahmed

13 June 2025

Background:Envenomation by poisonous creatures is a major global cause of mortality. Its concomitant impact on the postmortem corpse decomposition and associated insect succession pattern is still poorly understood. Purpose of the study: This study c...

  • Article
  • Open Access
1 Citations
1,570 Views
17 Pages

Transcriptome-Wide Identification of Neuropeptides and Neuropeptide Receptors in the Twenty-Eight-Spotted Ladybird Henosepilachna vigintioctopunctata

  • Quanxing Lei,
  • Ziming Wang,
  • Shuangyan Yao,
  • Aili Lin,
  • Yunhui Zhang,
  • Chengxian Sun,
  • Xiaoguang Liu,
  • Mengfang Du,
  • Xiaoming Liu and
  • Shiheng An

13 June 2025

The ladybird beetle, Henosepilachna vigintioctopunctata, is an oligophagous pest with significant economic impact. This pest causes considerable economic damage on numerous Solanaceae crops. Neuropeptides, along with their designated receptors, play...

  • Article
  • Open Access
1,992 Views
15 Pages

Predicting the Current and Future Habitat Distribution for an Important Fruit Pest, Grapholita dimorpha Komai (Lepidoptera: Tortricidae), Using an Optimized MaxEnt Model

  • Li Huang,
  • Shichao Zuo,
  • Yiqi Huo,
  • Lizong Hu,
  • Zhengbing Wang,
  • Jiahui Zhang,
  • Jin Liu,
  • Weili Ding,
  • Keshi Ma and
  • Mingsheng Yang

12 June 2025

The Grapholita dimorpha is one of the significant borer pests that primarily damage plum, pear, and apple trees, often resulting in substantial economic losses in fruit production. However, the potential distribution range of this economically import...

  • Article
  • Open Access
1,865 Views
10 Pages

Glycosidase Isoforms in Honey and the Honey Bee (Apis mellifera L.): Differentiating Bee- and Yeast-Derived Enzymes and Implications for Honey Authentication

  • Ratko Pavlović,
  • Sanja Stojanović,
  • Marija Pavlović,
  • Nenad Drulović,
  • Miroslava Vujčić,
  • Biljana Dojnov and
  • Zoran Vujčić

12 June 2025

The enzymes in honey can originate not only from bees and the plants from which the bees collect pollen and nectar but also from feed provided by beekeepers. Enzymes that hydrolyze sucrose—present in honey (α-glucosidase) or honey adulter...

  • Article
  • Open Access
3 Citations
2,405 Views
12 Pages

11 June 2025

Insects rely on their innate immune system to defend against pathogens, and the Toll signaling pathway plays an important role in immune regulation. Our previous studies have shown that BmToll9-1 functions as a positive regulator in the Toll pathway....

  • Article
  • Open Access
2 Citations
2,215 Views
12 Pages

Invasion Dynamics and Migration Patterns of Fall Armyworm (Spodoptera frugiperda) in Shaanxi, China

  • Zhanfeng Yan,
  • Xiaojun Feng,
  • Xing Wang,
  • Xiangqun Yuan,
  • Yongjun Zhang,
  • Daibin Yang,
  • Kanglai He,
  • Feizhou Xie,
  • Zhenying Wang and
  • Yiping Li

11 June 2025

The fall armyworm (Spodoptera frugiperda) is a highly invasive agricultural pest that has caused significant damage to maize and other crops since its initial detection in China in 2019. Understanding its invasion dynamics, migration patterns, geneti...

  • Article
  • Open Access
1,767 Views
16 Pages

Histopathological and Transcriptional Changes in Silkworm Larval Gonads in Response to Chlorfenapyr Exposure

  • Tao Li,
  • Changxiong Hu,
  • Zenghu Liu,
  • Qiongyan Li,
  • Yonghui Fan,
  • Pengfei Liao,
  • Min Liu,
  • Weike Yang,
  • Xingxing Li and
  • Zhanpeng Dong

11 June 2025

Chlorfenapyr is a widely used insecticide known to harm non-target insects, but its effects on reproductive development in the silkworm (Bombyx mori L.) remain incompletely understood. In this study, we investigated the histopathological and transcri...

  • Article
  • Open Access
2,068 Views
22 Pages

The Thoracic Anatomy of Two Flightless Chrysolina Species (Coleoptera: Chrysomelidae)

  • Sipei Liu,
  • Xiaokun Liu,
  • Xieshuang Wang,
  • Wenjie Li,
  • Xin Liu and
  • Siqin Ge

11 June 2025

Flight loss has independently evolved across nearly all winged insect orders. Comparing the thoracic structures of flightless insects with those of their flight-capable relatives can reveal key characteristics linked with flight. Although flight loss...

  • Review
  • Open Access
1 Citations
3,293 Views
18 Pages

11 June 2025

Honeybees are vital for pollination and the overall health of ecosystems. Since the 18th century, the intricate biology of honeybees has been a subject of scientific inquiry. Understanding their biological and behavioral characteristics is essential...

  • Article
  • Open Access
1,695 Views
13 Pages

10 June 2025

Respiration rates in insects are critical for survival and environmental adaptation, being influenced by developmental stages, environmental conditions, and the regulation of mitochondrial protein-coding genes. However, methods for field-based measur...

  • Review
  • Open Access
1 Citations
2,457 Views
14 Pages

10 June 2025

Ambrosia beetles, known for their symbiotic relationship with fungi cultivated within the tissues of host trees, have become significant pests, particularly when they serve as vectors for pathogenic fungi such as Raffaelea lauricola. Given the regula...

  • Article
  • Open Access
1,654 Views
15 Pages

10 June 2025

The invasive pest Frankliniella occidentalis (western flower thrip, WFT) severely impacts agricultural production. RNA interference (RNAi) has emerged as a viable alternative to chemical control methods. Trehalose-6-phosphate synthase (TPS) is an imp...

  • Article
  • Open Access
2,815 Views
23 Pages

Mating Behavior of Cyclocephala barrerai Martínez (Coleoptera: Melolonthidae)

  • Abraham Sanchez-Cruz,
  • Patricia Villa-Ayala and
  • Alfredo Jiménez-Pérez

10 June 2025

Our study aimed to characterize mating behavior in virgin and once-mated males and females of Cyclocephala barrerai. We reared the specimens under laboratory conditions and recorded the age at which the organisms began their reproductive activity and...

  • Article
  • Open Access
1 Citations
2,584 Views
20 Pages

Reproductive Senescence in the Pollinator, Megachile rotundata

  • Jacob B. Pithan,
  • Brooke L. Kohler,
  • Arun Rajamohan and
  • Kendra J. Greenlee

10 June 2025

The disposable soma theory posits that organisms allocate limited resources between reproduction, maintenance, and growth, resulting in trade-offs, particularly as they age. In this study, we examined age-related reproductive senescence in Megachile ...

  • Article
  • Open Access
1,181 Views
13 Pages

10 June 2025

In insect–microbe symbiosis, understanding the diversity of associated bacteria is crucial. DNA-dependent sequence methods are widely used to assess microbial diversity in insects, but they cannot distinguish between live and dead microbes. In...

  • Article
  • Open Access
5 Citations
2,019 Views
20 Pages

High-Precision Stored-Grain Insect Pest Detection Method Based on PDA-YOLO

  • Fuyan Sun,
  • Zhizhong Guan,
  • Zongwang Lyu and
  • Shanshan Liu

10 June 2025

Effective stored-grain insect pest detection is crucial in grain storage management to prevent economic losses and ensure food security throughout production and supply chains. Existing detection methods suffer from issues such as high labor costs, e...

  • Article
  • Open Access
5 Citations
1,835 Views
26 Pages

9 June 2025

Sitophilus zeamais, a major pest of stored grains, causes significant post-harvest losses and challenges effective control. While synthetic insecticides pose risks of resistance and toxicity, essential oils (EOs) offer a safer alternative. However, t...

  • Article
  • Open Access
1 Citations
1,723 Views
23 Pages

9 June 2025

The Colorado potato beetle is a notorious agricultural pest with remarkable adaptability and insecticide resistance. This study conducted comprehensive transcriptome analysis across multiple developmental stages and tissues, focusing on the cytochrom...

  • Article
  • Open Access
2,131 Views
20 Pages

9 June 2025

Chemosensory systems play a pivotal role in insect survival and reproduction by mediating the detection of volatile organic compounds in the environment. Protaetia brevitarsis (Coleoptera: Scarabaeidae), a phytophagous pest widely distributed across...

  • Article
  • Open Access
1 Citations
1,424 Views
15 Pages

8 June 2025

Organic farming can help mitigate the negative impacts of agriculture on biodiversity, but its effects remain controversial and poorly understood for many taxa, especially from a genetic perspective, where major knowledge gaps persist. This study inv...

  • Article
  • Open Access
1,790 Views
16 Pages

8 June 2025

The northeast region of China plays a crucial role in crop production. The leaf beetle Monolepta hieroglyphica (Motschulsky, 1858) (Coleoptera: Chrysomelidae) has emerged as a potential threat to food security in the region. With a wide distribution...

  • Article
  • Open Access
1 Citations
2,302 Views
19 Pages

Cytogenetic and Molecular Characterization of Sphaerophoria rueppellii (Diptera, Syrphidae)

  • Pedro Lorite,
  • José M. Rico-Porras,
  • Teresa Palomeque,
  • Mª Ángeles Marcos-García,
  • Diogo C. Cabral-de-Mello and
  • Pablo Mora

8 June 2025

Sphaerophoria rueppellii is a Palearctic hoverfly widely used as a native biocontrol agent against aphid pests in Mediterranean agroecosystems. In this study, we present a cytogenetic analysis and characterization of the mitochondrial genome of this...

  • Article
  • Open Access
2 Citations
1,524 Views
26 Pages

Diverse Sublethal Effects of a Common Fungicide Impact the Behavior and Physiology of Honey Bees

  • Xufeng Zhang,
  • Qian Cao,
  • Feng Wang,
  • Yinyin Du,
  • Wen Zhao,
  • Yuan Guo and
  • Olav Rueppell

8 June 2025

Honey bees and other pollinators are key to functioning natural and managed ecosystems. However, their health is threatened by many factors, including pesticides. Spraying fungicides during flowering of fruit trees is widespread even though it direct...

  • Article
  • Open Access
1 Citations
1,395 Views
13 Pages

7 June 2025

The Dolomites (European Alps) are a UNESCO World Heritage Site known to harbor distinctive communities of carabid beetles adapted to high-altitude environments, whose composition is shaped mainly by landform and habitat type. We aimed to assess the c...

  • Article
  • Open Access
1,275 Views
19 Pages

Comprehensive Analysis of the UGT Gene Superfamily in Spodoptera frugiperda

  • Yang Liu,
  • Minghui Guan,
  • Kunliang Zou,
  • Tonghan Wang,
  • Haiyang Wang,
  • Lu Sun,
  • Bo Feng,
  • Jiali Ding,
  • Xiang Gao and
  • Junli Du
  • + 2 authors

6 June 2025

UDP-glycosyltransferases (UGTs) are widely distributed enzymes in living organisms that catalyze the transfer of glycosyl groups from donor molecules to acceptor molecules’ glycoside ligands. These enzymes are pivotal for detoxifying and elimin...

  • Article
  • Open Access
2 Citations
2,809 Views
25 Pages

6 June 2025

The tropical house cricket (Gryllodes sigillatus) can convert organic diets formulated from weeds and agro by-products into high-quality biomass. This study assessed the potential of diets developed from weeds and agro by-products as a feed source fo...

  • Article
  • Open Access
2,020 Views
17 Pages

6 June 2025

Leishmaniasis, a parasitic disease transmitted by sand flies, poses a significant global health threat. Chemical repellents and insecticides are widely used for protection, but prolonged use has led to resistance, reduced efficacy, and environmental...

  • Article
  • Open Access
1,693 Views
13 Pages

6 June 2025

Madhya Pradesh, a biodiversity-rich state in central India, reports sporadic non-indigenous leishmaniasis cases. Systematic entomological surveillance as part of molecular xenomonitoring in sand flies led to the discovery of a new species, Sergentomy...

  • Article
  • Open Access
4,176 Views
26 Pages

5 June 2025

The genus Tineobius Ashmead (Hymenoptera: Eupelmidae) is recorded for the first time in China based on two new species, Tineobius (Tineobius) elpisios Li & Peng sp. nov. and Tineobius (Tineobius) victor Li & Peng sp. nov., along with two newl...

  • Article
  • Open Access
1,478 Views
14 Pages

5 June 2025

Temperature is a critical factor affecting the development and population dynamics of many organisms. An organism’s ability to withstand extreme temperature events, such as heat waves, will become increasingly important as the severity, duratio...

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Insects - ISSN 2075-4450