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Insects, Volume 16, Issue 5

2025 May - 111 articles

Cover Story: Despite their importance for monitoring and conservation, modern inventories of Lepidoptera (butterflies and moths) are lacking for countries in south-eastern Europe, including the endemism-rich island of Crete. We address this gap by critically analyzing the species inventory, incorporating validated publications, photographically documented citizen science observations, and extensive original sampling, including DNA barcode analysis of more than half of the recorded species. The checklist contains 1230 species, including 125 new faunistic records for Crete and/or Greece. Conversely, 212 previously reported species had to be removed as likely invalid. It is noteworthy that almost 10 % of the fauna lacks a clear taxonomic classification, highlighting the urgent need for integrative taxonomic research. View this paper
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Articles (111)

  • Article
  • Open Access
2 Citations
1,947 Views
24 Pages

21 May 2025

The morphology, number, and distribution of antennal sensilla differ between males and females, reflecting adaptations to sex-specific ecological roles and life histories. In this study, scanning electron microscopy was employed to examine the antenn...

  • Article
  • Open Access
1,342 Views
13 Pages

Population Genomics and Morphology Provide Insights into the Conservation and Diversity of Apis laboriosa

  • Ri Liu,
  • Xuntao Ma,
  • Longfu Zhang,
  • Kang Lai,
  • Changbin Shu,
  • Bin Wang,
  • Mingwang Zhang and
  • Mingxian Yang

21 May 2025

In recent decades, honeybee populations have declined, dramatically owing to destructive honey harvesting practices and the loss of foraging grounds and nesting sites. Among them, Apis laboriosa Smith, 1871 (Hymenoptera, Apidae), an important pollina...

  • Article
  • Open Access
5 Citations
1,783 Views
22 Pages

21 May 2025

Due to target diversity, life-cycle variations, and complex backgrounds, traditional pest detection methods often struggle with accuracy and efficiency. This study introduces RDW-YOLO, an improved pest detection algorithm based on YOLO11, featuring t...

  • Article
  • Open Access
2 Citations
1,034 Views
15 Pages

21 May 2025

In the silkworm, 20-hydroxyecdysone (20E) induces apoptosis and autophagy, driving larval organ degeneration and remodeling. This mechanism may be a potential target for eco-friendly strategy for insect pests. However, a major challenge in harnessing...

  • Article
  • Open Access
1,174 Views
17 Pages

20 May 2025

This study reports the first complete mitochondrial genomes of three egg parasitoid wasps parasitizing Nilaparvata lugensPseudoligosita nephotetticum, Anagrus frequens, and Anagrus nilaparvatae. Genome sizes ranged from 15,429 to 15,889 bp, wi...

  • Article
  • Open Access
2,426 Views
12 Pages

Adulticidal and Repellent Activity of Essential Oils from Three Cultivated Aromatic Plants Against Musca domestica L.

  • Gabriela Antonieta Oyarce,
  • Patricia Loyola,
  • Michelle Iubini-Aravena,
  • Álvaro Romero,
  • J. Concepción Rodríguez-Maciel,
  • José Becerra and
  • Gonzalo Silva-Aguayo

20 May 2025

The house fly, Musca domestica L., is a pest of great medical and agricultural importance, serving as a vector for various diseases and undermining the quality of agricultural products. Traditionally, synthetic insecticides have been the primary mean...

  • Article
  • Open Access
1,311 Views
21 Pages

20 May 2025

Pear psylla (Cacopsylla chinensis), a major pear tree pest widely distributed in China, is increasingly affecting the productivity of orchards. This species exhibits seasonal polyphenism with two distinct forms, namely, a summer form and a winter for...

  • Article
  • Open Access
2 Citations
967 Views
19 Pages

20 May 2025

The bio-prospecting of bioactive phytochemicals from invasive flora presents a sustainable paradigm for the ecologically conscious management of major invasive pest S. invicta. Ageratina adenophora, while recognized for its allelopathic insecticidal...

  • Article
  • Open Access
1,251 Views
20 Pages

Ultrastructural Characterization of Developmental Stages and Head Sensilla in Portici okadai, Vector of Thelazia callipaeda

  • Da Sun,
  • Yang Luo,
  • Yikang Wang,
  • Hongle Cui,
  • Yanting Gou,
  • Juan Zhou,
  • Bo Luo,
  • Hui Liu,
  • Rong Yan and
  • Lingjun Wang

20 May 2025

Phortica okadai, a vector of Thelazia callipaeda, is associated with an increasing incidence of thelaziasis. The complex habitat and chemosensory system of P. okadai are critical for its proliferation and expansion. However, ultrastructural data acro...

  • Article
  • Open Access
3 Citations
1,970 Views
16 Pages

Filling the Spring Gap in Southern Australia: Seasonal Activity of Four Dung Beetle Species Selected to Be Imported from Morocco

  • Hasnae Hajji,
  • Abdellatif Janati-Idrissi,
  • Alberto Zamprogna,
  • José Serin,
  • Jean-Pierre Lumaret,
  • Nassera Kadiri,
  • Saleta Pérez Vila,
  • Patrick V. Gleeson,
  • Jane Wright and
  • Valérie Caron

20 May 2025

Dung beetles are important ecosystem engineers, as they utilize the excrement produced by animals. For nearly 60 years, several species of dung beetle have been introduced to Australia to help mitigate the problems caused by the accumulation of lives...

  • Article
  • Open Access
1,429 Views
15 Pages

19 May 2025

20-hydroxyecdysone (20E) is essential for insect development and diapause. Ecdysone receptor (EcR) and ultraspiracle (USP) proteins are crucial regulators of 20E signaling. To explore their potential roles in the development of Sitodiplosis mosellana...

  • Article
  • Open Access
1,267 Views
12 Pages

Phylogenetic Analysis of the Family Lepidostomatidae (Trichoptera: Integripalpia) Using Whole Mitochondrial Genomes

  • Xinyu Ge,
  • Jingyuan Wang,
  • Zhen Deng,
  • Lu Chai,
  • Wei Cao,
  • Wenbin Liu,
  • Jiwei Zhang and
  • Chuncai Yan

19 May 2025

Lepidostomatidae is a relatively large family of the infraorder Plenitentoria (Integripalpia), comprising four fossil genera and seven extant genera. Lepidostomatid adults exhibit pronounced sexual dimorphism and have thus been referred to as the &ls...

  • Article
  • Open Access
1,959 Views
17 Pages

19 May 2025

With increasing demand for stingless bee honey, meliponiculture has gained widespread attention. Heterotrigona itama is one of the most economically important species. However, excessive exploitation for commercial purposes has led to population decl...

  • Article
  • Open Access
2,946 Views
14 Pages

19 May 2025

Calyptra Ochsenheimer, 1816 is an important genus of fruit piercers and blood feeders with 18 described species worldwide. Both sexes of all Calyptra species pierce fruit. Adults feed upon and damage soft-skinned (e.g., Rubus and Vitis) and thicker-s...

  • Article
  • Open Access
1,242 Views
12 Pages

Effect of Pyrethroids on the Colony Growth and Metabolic Activity of Entomopathogenic Fungi of the Beauveria Genus

  • Anna Majchrowska-Safaryan,
  • Sylwia Różalska,
  • Cezary Tkaczuk and
  • Monika Nowak

18 May 2025

Pyrethroids are chemical insecticides used on a large scale in agriculture, horticulture, and forest protection. In order to reduce their use in IPM, alternative methods of controlling insect pests are introduced, such as the use of biopesticides bas...

  • Article
  • Open Access
1,377 Views
10 Pages

18 May 2025

Bacillus thuringiensis (Bt) is an efficacious biocontrol bacterium known for producing various toxins, such as crystal toxins, which disrupt the midgut epithelium of pest larvae, leading to larval mortality. However, the development of resistance to...

  • Article
  • Open Access
2,204 Views
10 Pages

16 May 2025

Maize (Zea mays) is a critical staple crop whose post-harvest losses, predominantly due to infestations by the maize weevil, Sitophilus zeamais, threaten food security. This study explores the possibility of utilizing S. zeamais, traditionally known...

  • Article
  • Open Access
1,470 Views
17 Pages

Preliminary Development of Global–Local Balanced Vision Transformer Deep Learning with DNA Barcoding for Automated Identification and Validation of Forensic Sarcosaphagous Flies

  • Yixin Ma,
  • Lin Niu,
  • Bo Wang,
  • Dianxin Li,
  • Yanzhu Gao,
  • Shan Ha,
  • Boqing Fan,
  • Yixin Xiong,
  • Bin Cong and
  • Jianqiang Deng
  • + 1 author

16 May 2025

Morphological classification is the gold standard for identifying necrophilous flies, but its complexity and the scarcity of experts make accurate classification challenging. The development of artificial intelligence for autonomous recognition holds...

  • Review
  • Open Access
7 Citations
4,406 Views
27 Pages

Insect Pest Control from Chemical to Biotechnological Approach: Constrains and Challenges

  • Stefano Civolani,
  • Massimo Bariselli,
  • Riccardo Osti and
  • Giovanni Bernacchia

15 May 2025

The large growth in the global population requires new solutions for the control of harmful insects that compete for our food. Changing regulatory requirements and public perception, together with the continuous evolution of resistance to conventiona...

  • Article
  • Open Access
1 Citations
1,029 Views
13 Pages

Selection and Validation of Stable Reference Genes for RT-qPCR in Scotogramma trifolii (Lepidoptera: Noctuidae)

  • Anpei Yang,
  • Hang Zhang,
  • Weiwei Bai,
  • Ruifeng Ding,
  • Weipeng Li and
  • Guangkuo Li

15 May 2025

The clover cutworm, Scotogramma trifolii Rottemberg (Lepidoptera: Noctuidae), is a globally distributed polyphagous pest causing significant economic losses to agricultural crops. RT-qPCR is a gold-standard technique for gene expression analysis, yet...

  • Article
  • Open Access
1 Citations
2,062 Views
18 Pages

15 May 2025

In order to examine the phylogeny and evolutionary history of the subfamily Notodontinae (Noctuoidea: Notodontidae), a molecular systematic study was conducted, mainly based on mitochondrial protein-coding genes (PCGs) generated by high-throughput se...

  • Article
  • Open Access
820 Views
9 Pages

15 May 2025

Pheromone-baited traps are commonly used to monitor and detect moths. Traps and lures are often compared to each other to identify the optimum set up and lure dosage. However, it is also important to understand the efficiency of a trap in capturing t...

  • Article
  • Open Access
1 Citations
1,505 Views
20 Pages

Multigeneration Sublethal Chlorantraniliprole Treatment Disrupts Nutritional Metabolism and Inhibits Growth, Development, and Reproduction of Phthorimaea absoluta

  • Lun Li,
  • Zunzun Jia,
  • Kaiyun Fu,
  • Xinhua Ding,
  • Weihua Jiang,
  • Xiaowu Wang,
  • Tursun. Ahmat,
  • Jiahe Wu,
  • Yutong Wen and
  • Hongying Hu
  • + 2 authors

15 May 2025

Phthorimaea absoluta, an important pest of tomato crops, has reportedly developed high levels of resistance to the insecticide chlorantraniliprole, which has a unique mode of action and high efficacy. This study evaluated the sustained multigeneratio...

  • Article
  • Open Access
2 Citations
3,796 Views
14 Pages

Mating Disruption of Helicoverpa armigera (Lepidoptera: Noctuidae) Using Yeast-Derived Pheromones in Cotton Fields

  • Dimitris Raptopoulos,
  • Petri-Christina Betsi,
  • Neoklis Manikas,
  • Irina Borodina and
  • Maria Konstantopoulou

15 May 2025

The use of insect sex pheromones as an alternative technology for pest control in agriculture and forestry offers a promising solution. The development of a novel technology for the biological production of pheromones through yeast fermentation signi...

  • Article
  • Open Access
1 Citations
1,351 Views
20 Pages

Suitability of Three Trunk Traps for Capturing Larvae of Lymantria dispar (L.) (Lepidoptera, Erebidae)

  • Tanja Bohinc,
  • Paraskevi Agrafioti,
  • Stelios Vasilopoulos,
  • Evagelia Lampiri,
  • Maria C. Boukouvala,
  • Anna Skourti,
  • Demeter Lorentha S. Gidari,
  • Nickolas G. Kavallieratos,
  • Xavier Pons and
  • Stanislav Trdan
  • + 5 authors

15 May 2025

In this paper, we present the results of field investigations on the suitability of three types of trunk traps (‘Commercial 1’, ‘Commercial 2’, and ‘Prototype’) for capturing spongy moth (Lymantria dispar) larvae i...

  • Article
  • Open Access
2 Citations
1,172 Views
12 Pages

Effects of Saturated Soil Moisture on Fall Armyworm Pupal Development

  • Tianqi Tian,
  • Yingyan Zhai,
  • Zhijie Chen,
  • Yiwei Yang and
  • Bo Hong

14 May 2025

Spodoptera frugiperda, known as the fall armyworm (FAW), a major invasive pest in corn, is rapidly spreading all over the world. Similarly to other Lepidoptera insects, FAW pupae usually develop in soil. Therefore, the soil moisture level is expected...

  • Article
  • Open Access
1 Citations
1,331 Views
26 Pages

14 May 2025

The article presents integrative research of the perlodid genus Perlodinella in Qinghai Province, northwestern China. P. tatunga Wu, 1973 is considered a junior synonym of P. kozlovi Klapálek, 1912, with a further description of intraspecific...

  • Article
  • Open Access
1 Citations
1,788 Views
19 Pages

14 May 2025

Aprostocetus nitens Prinsloo & Kelly (Hymenoptera: Eulophidae) was identified as one of four hymenopteran ectoparasitoids utilizing three erythrina gall wasps, Quadrastichus bardus, Q. erythrinae, and Q. gallicola) (Hymenoptera: Eulophidae)...

  • Review
  • Open Access
3 Citations
4,646 Views
18 Pages

The Role of Red Wood Ants (Formica rufa Species Group) in Central European Forest Ecosystems—A Literature Review

  • Ágnes Fürjes-Mikó,
  • Sándor Csősz,
  • Márton József Paulin and
  • György Csóka

13 May 2025

Red wood ants (RWA), belonging to the Formica rufa species group, play a crucial and fascinating role in Central Europe’s forest ecosystems. They have a high variety of effects, which they exert around their nests. Their generalist feeding on p...

  • Article
  • Open Access
2 Citations
1,568 Views
16 Pages

A Little Peek May Be Enough: How Small Hive Beetle Estimates Can Help Address Immediate Colony Management Needs

  • Ethel M. Villalobos,
  • Luis Medina Medina,
  • Zhening Zhang,
  • Scott Nikaido,
  • Emanuel Miranda,
  • Jason Wong,
  • Jessika Santamaria and
  • Micaela Buteler

13 May 2025

Due to the ongoing global spread of the small hive beetle (SHB), Aethina tumida, there is a significant need for detection and practical management strategies against this pest. The standard inspection strategies for SHBs involve (1) detailed visual...

  • Review
  • Open Access
4 Citations
2,770 Views
17 Pages

13 May 2025

There has been an unprecedented demand for livestock production due to factors such as the ever-increasing population, limited resources (land, water, feed, etc.), and changing human lifestyles. Moreover, due to the interconnected nature of the world...

  • Article
  • Open Access
1 Citations
1,265 Views
18 Pages

microRNA Targeting Cytochrome P450 Is Involved in Chlorfenapyr Tolerance in the Silkworm, Bombyx mori (Lepidoptera: Bombycidae)

  • Ying Shao,
  • Jian-Hao Ding,
  • Wang-Long Miao,
  • Yi-Ren Wang,
  • Miao-Miao Pei,
  • Sheng Sheng and
  • Zhong-Zheng Gui

12 May 2025

We first measured the content of chlorfenapyr and tralopyril in silkworm larvae using HPLC, revealing that chlorfenapyr can be biotransformed into tralopyril in silkworms. Then, a differential transcriptomic database of small RNA was constructed thro...

  • Review
  • Open Access
2,846 Views
14 Pages

The Baluchistan Melon Fly, Myiopardalis pardalina Bigot: Biology, Ecology, and Management Strategies

  • Junyan Liu,
  • Yidie Xu,
  • Mengbo Guo,
  • Kaiyun Fu,
  • Xinhua Ding,
  • Sijia Yu,
  • Xinyi Gu,
  • Wenchao Guo and
  • Jianyu Deng

11 May 2025

The Baluchistan melon fly (Myiopardalis pardalina) is a highly invasive tephritid pest. It poses a critical threat to global cucurbit production, with crop losses exceeding 90% during outbreaks. This review synthesises current research on the pest&rs...

  • Article
  • Open Access
5 Citations
5,524 Views
20 Pages

Climate Change: A Major Factor in the Spread of Aedes aegypti (Diptera: Culicidae) and Its Associated Dengue Virus

  • Shahid Majeed,
  • Waseem Akram,
  • Muhammad Sufyan,
  • Asim Abbasi,
  • Sidra Riaz,
  • Shahla Faisal,
  • Muhammad Binyameen,
  • Muhammad I. Bashir,
  • Shahzad Hassan and
  • Olga D. Kucher
  • + 3 authors

11 May 2025

Climate change is thought to be responsible for the spread of various vector-borne diseases. The current study was conducted to evaluate the impact of different temperature and relative humidity regimes on the developmental stages of the yellow fever...

  • Review
  • Open Access
1,802 Views
17 Pages

Conservation of Apis mellifera mellifera L. in the Middle Ural: A Review of Genetic Diversity, Ecological Adaptation, and Breeding Perspectives

  • Olga Frunze,
  • Alexander V. Petukhov,
  • Anna Z. Brandorf,
  • Mikhail K. Simankov,
  • Hyunjee Kim and
  • Hyung-Wook Kwon

11 May 2025

The European dark bee is well adapted to cold winters and short summers. However, threats from habitat loss, pests, and hybridization with southern bees pose significant challenges to its populations. The Perm region (Middle Ural, Russia) hosts a dis...

  • Article
  • Open Access
3 Citations
2,080 Views
21 Pages

Rare Chromosomal Uniformity in Black Flies of the Simulium striatum Species Group (Diptera: Simuliidae)

  • Peter H. Adler,
  • Sergey Vlasov,
  • Yao-Te Huang,
  • Upik K. Hadi,
  • Khamla Inkhavilay,
  • Banchai Malavong,
  • Varvara Topolenko,
  • Bhuvadol Gomontean,
  • Waraporn Jumpato and
  • Pairot Pramual
  • + 6 authors

10 May 2025

We conducted a comparative chromosomal analysis of 10 nominal species and 3 unidentified species in the Simulium striatum group from six countries. A total of 66 chromosomal rearrangements were found, of which 78.8% were inversions. The group is defi...

  • Article
  • Open Access
1 Citations
1,385 Views
13 Pages

An Optimized Bioassay System for the Striped Flea Beetle, Phyllotreta striolata

  • Liyan Yao,
  • Xinhua Pu,
  • Yuanlin Wu,
  • Ke Zhang,
  • Alexander Berestetskiy,
  • Qiongbo Hu and
  • Qunfang Weng

10 May 2025

The striped flea beetle (SFB), Phyllotreta striolata, is a major pest of Brassicaceae crops, causing substantial yield losses worldwide. Effective biocontrol strategies, particularly the development of mycoinsecticides, require the identification of...

  • Review
  • Open Access
2 Citations
2,714 Views
13 Pages

Strategies to Mitigate the Adverse Impacts of Viral Infections on Honey Bee (Apis mellifera L.) Colonies

  • Ivana Tlak Gajger,
  • Hossam F. Abou-Shaara and
  • Maja Ivana Smodiš Škerl

10 May 2025

Honey bees (Apis mellifera) play a crucial role in global food production through the pollination of various crops. These vital insects are susceptible to a range of viral pathogens that can disrupt their normal behavior and physiology, ultimately af...

  • Article
  • Open Access
10 Citations
2,298 Views
20 Pages

Food waste containing meat and fish presents a considerable environmental challenge due to regulatory constraints preventing its use in industrial insect farming. Although substrates derived from meat and fish are not currently approved for industria...

  • Article
  • Open Access
1,224 Views
17 Pages

Bacillus thuringiensis is widely utilized as a microbial insecticide due to its production of parasporal crystals during the spore-forming stage. However, lower fermentation efficiency coupled with elevated production costs limit its broad applicatio...

  • Review
  • Open Access
2 Citations
4,969 Views
26 Pages

Insect mating behaviors are complex, diverse, and primarily regulated by neuropeptides and their receptors. Neuropeptides are peptide signaling molecules mainly secreted by insects’ central nervous system (CNS) to reach target organs. A substan...

  • Article
  • Open Access
4 Citations
3,399 Views
19 Pages

The nutritional value of pollen for honeybees varies significantly depending on its source. This study examines the nutrient composition of three types of bee-collected pollen—Maize bee-collected pollen (MBP), Lotus bee-collected pollen (LBP),...

  • Article
  • Open Access
1 Citations
1,423 Views
19 Pages

Euplatypus parallelus is a polyphagous pest capable of harming multiple plant species. Adult beetles invade tree trunks by boring holes, which negatively impacts the trees’ growth and may result in tree death. E. parallelus depends on plant vol...

  • Article
  • Open Access
1 Citations
1,207 Views
23 Pages

The hazelnut weevil larvae (Curculio dieckmanni) is a major pest of nut weevils, spending part of its life cycle in the soil and causing significant damage to hazelnut crops. Moreover, its concealed feeding behavior complicates effective control with...

  • Article
  • Open Access
2 Citations
1,453 Views
15 Pages

Genomic Analysis Reveals the Role of New Genes in Venom Regulatory Network of Parasitoid Wasps

  • Bo Zhang,
  • Yifan Bu,
  • Jiqiang Song,
  • Bo Yuan,
  • Shan Xiao,
  • Fang Wang,
  • Qi Fang,
  • Gongyin Ye,
  • Yi Yang and
  • Xinhai Ye

New genes play a critical role in phenotypic diversity and evolutionary innovation. Parasitoid wasps, a highly abundant and diverse group of insects, parasitize other arthropods and exhibit remarkable evolutionary adaptations, such as evading host im...

  • Article
  • Open Access
1 Citations
1,776 Views
17 Pages

Environmental Factors Determining the Distribution Pattern of Chironomidae in Different Types of Freshwater Habitats

  • Nataša Popović,
  • Jelena Đuknić,
  • Nikola Marinković,
  • Bojana Tubić,
  • Ana Atanacković,
  • Djuradj Milošević and
  • Maja Raković

Chironomidae are characterised by cosmopolitan distribution, high abundance and diversity in different aquatic environments, which makes them ideal for studying changes in freshwater ecosystems. To understand the environmental factors influencing chi...

  • Article
  • Open Access
1,545 Views
21 Pages

Species of the widespread Obsoletus Complex (Culicoides subgenus Avarita Fox, 1955) have been implicated as potential key vectors during the bluetongue and Schmallenberg epidemics in Central Europe in 2006 and 2012. Although extensive efforts have be...

  • Article
  • Open Access
1,768 Views
22 Pages

Psyllids (Hemiptera: Psylloidea) are the main vectors of various phloem-limited plant pathogens, including ‘Candidatus Liberibacter’ species. ‘Candidatus Liberibacter solanacearum’ (CLso) has been associated with various plant...

  • Article
  • Open Access
3 Citations
3,366 Views
24 Pages

Species Richness and Distribution of Calliphoridae Along an Elevation Gradient in Sicily (Italy) and Ecuador

  • M. Denise Gemmellaro,
  • Gail S. Anderson,
  • George C. Hamilton,
  • Mariela Domínguez-Trujillo and
  • Lauren M. Weidner

Blow flies (Diptera: Calliphoridae) are among the first insects to arrive on a corpse, and so they are particularly important in forensic entomology. To use blow flies in forensic investigations, there must be information available on their species d...

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Insects - ISSN 2075-4450