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130 Results Found

  • Article
  • Open Access
10 Citations
7,855 Views
13 Pages

Characteristics and Absorption Rate of Whey Protein Hydrolysates Prepared Using Flavourzyme after Treatment with Alcalase and Protamex

  • Yeok Boo Chang,
  • Hyeongyeong Kim,
  • Se Kyung Lee,
  • Hye-Jin Kim,
  • A-Hyun Jeong,
  • Hyung Joo Suh and
  • Yejin Ahn

6 December 2023

The purpose of this study was to evaluate the physicochemical properties of whey protein hydrolysate and determine changes in absorption rate due to enzymatic hydrolysis. The molecular weight distribution analysis of whey protein concentrate (WPC) an...

  • Article
  • Open Access
28 Citations
4,714 Views
22 Pages

In Vitro Characterisation of the Antioxidative Properties of Whey Protein Hydrolysates Generated under pH- and Non pH-Controlled Conditions

  • Thanyaporn Kleekayai,
  • Aurélien V. Le Gouic,
  • Barbara Deracinois,
  • Benoit Cudennec and
  • Richard J. FitzGerald

5 May 2020

Bovine whey protein concentrate (WPC) was hydrolysed under pH-stat (ST) and non pH-controlled (free-fall, FF) conditions using Debitrase (DBT) and FlavorPro Whey (FPW). The resultant whey protein hydrolysates (WPHs) were assessed for the impact of hy...

  • Article
  • Open Access
20 Citations
3,460 Views
15 Pages

Papain and pepsin-hydrolyzed whey protein (PAH and PEH, respectively) were prepared and characterized for its degree of hydrolysis, chemical constituents (amino acid and peptides) and antioxidant activity. A field experiment was conducted at El Salhe...

  • Article
  • Open Access
18 Citations
3,616 Views
17 Pages

12 August 2022

Hydrolysis of proteins leads to the release of bioactive peptides with positive impact on human health. Peptides exhibiting antihypertensive properties (i.e., inhibition of angiotensin-I-converting enzyme) are commonly found in whey protein hydrolysa...

  • Article
  • Open Access
11 Citations
4,193 Views
11 Pages

Yoghurt-Type Gels from Skim Sheep Milk Base Enriched with Whey Protein Concentrate Hydrolysates and Processed by Heating or High Hydrostatic Pressure

  • Lambros Sakkas,
  • Maria Tzevdou,
  • Evangelia Zoidou,
  • Evangelia Gkotzia,
  • Anastasis Karvounis,
  • Antonia Samara,
  • Petros Taoukis and
  • Golfo Moatsou

12 August 2019

An objective of the present study was the enrichment of skim sheep yoghurt milk base with hydrolysates (WPHs) of whey protein concentrate (WP80) derived from Feta cheesemaking. Moreover, the use of high hydrostatic pressure (HP) treatment at 600 MPa/...

  • Article
  • Open Access
47 Citations
7,996 Views
14 Pages

7 March 2013

Pure whey protein isolate (WPI)-based cast films are very brittle due to its strong formation of protein cross-linking of disulphide bonding, hydrogen bonding as well as hydrophobic and electrostatic interactions. However, this strong cross-linking i...

  • Article
  • Open Access
22 Citations
3,580 Views
15 Pages

19 July 2022

The effects of whey protein hydrolysates (WPH) on myofibrillar protein (MP) oxidative stability and the aggregation behavior and the water-holding capacity of pork patties during freeze–thaw (F–T) cycles were investigated. During F–...

  • Article
  • Open Access
17 Citations
5,199 Views
15 Pages

Impact of Whey Protein Edible Coating Containing Fish Gelatin Hydrolysates on Physicochemical, Microbial, and Sensory Properties of Chicken Breast Fillets

  • Forouzan Sabzipour-Hafshejani,
  • Armin Mirzapour-Kouhdasht,
  • Diako Khodaei,
  • Mohammad Sadegh Taghizadeh and
  • Marco Garcia-Vaquero

18 August 2022

This study aims to research the impact of coatings containing whey protein (WP), fish gelatin hydrolysates (FGH), and both compounds together (WP + FGH) on the shelf-life of chicken breast fillets over the course of 16 days of cold storage (4 °C,...

  • Article
  • Open Access
28 Citations
5,017 Views
15 Pages

Development of Chitosan/Whey Protein Hydrolysate Composite Films for Food Packaging Application

  • Sawsan A. Al-Hilifi,
  • Orass T. Al-Ibresam,
  • Raqad R. Al-Hatim,
  • Rawdah M. Al-Ali,
  • Namrata Maslekar,
  • Yin Yao and
  • Vipul Agarwal

There is a significant drive towards the development of edible biocompatible films for food packaging application due to the environmental and health impacts of synthetic packaging materials. This has inspired the exploration of biodegradable natural...

  • Article
  • Open Access
857 Views
14 Pages

30 July 2025

Background: Response speed refers to an individual’s ability to perceive and react to harmful stimuli, which can vary due to genetics, neural regulation, and environmental factors. Our previous study demonstrated that whey protein hydrolysate w...

  • Article
  • Open Access
63 Citations
7,396 Views
16 Pages

2 August 2013

Whey protein isolate (WPI)-based cast films are very brittle, due to several chain interactions caused by a large amount of different functional groups. In order to overcome film brittleness, plasticizers, like glycerol, are commonly used. As a resul...

  • Article
  • Open Access
5 Citations
2,947 Views
16 Pages

29 July 2022

A phenylalanine (Phe)-restricted diet is indispensable for individuals suffering from phenylketonuria (PKU). Our previous study reported a low-Phe-containing whey protein hydrolysate (LPH) prepared from a selected whey protein hydrolysate (TA2H). Thi...

  • Article
  • Open Access
42 Citations
8,978 Views
16 Pages

1 November 2020

Sarcopenia, a loss of skeletal muscle mass and function, is prevalent in older people and associated with functional decline and mortality. Protein supplementation is necessary to maintain skeletal muscle mass and whey protein hydrolysates have the b...

  • Article
  • Open Access
12 Citations
4,908 Views
18 Pages

Whey Protein Hydrolysate Renovates Age-Related and Scopolamine-Induced Cognitive Impairment

  • Ning Ding,
  • Hanxiu Meng,
  • Chao Wu,
  • Wallace Yokoyama,
  • Hui Hong,
  • Yongkang Luo and
  • Yuqing Tan

28 February 2023

Whey protein and its hydrolysates are ubiquitously applied in the food system. However, their effect on cognitive impairment remains unclear. This study aimed to investigate the potential ability of whey protein hydrolysate (WPH) to ameliorate cognit...

  • Article
  • Open Access
6 Citations
2,500 Views
16 Pages

21 August 2023

Whey protein hydrolysate (WPH) has made a breakthrough in inhibiting oxidative deterioration and improving the quality of meat products during storage. Based on our previous study of extracting the most antioxidant active fraction I (FI, the molecula...

  • Communication
  • Open Access
21 Citations
5,384 Views
13 Pages

In Vitro Gut Fermentation of Whey Protein Hydrolysate: An Evaluation of Its Potential Modulation on Infant Gut Microbiome

  • Chunsong Feng,
  • Li Tian,
  • Hui Hong,
  • Quanyu Wang,
  • Xin Zhan,
  • Yongkang Luo and
  • Yuqing Tan

25 March 2022

Whey protein and its hydrolysate are ubiquitously consumed as nutritional supplements. This study aimed to evaluate the potential effect of whey protein hydrolysate (WPH) on the infant gut microbiome, which is more variable than that of adults. Colon...

  • Article
  • Open Access
17 Citations
13,673 Views
11 Pages

19 April 2018

To stimulate muscle protein synthesis, it is important to increase the plasma levels of essential amino acids (EAA), especially leucine, by ingesting proteins. Protein hydrolysate ingestion can induce postprandial hyperaminoacidemia; however, it is u...

  • Article
  • Open Access
26 Citations
4,377 Views
19 Pages

18 May 2023

The production of animal protein usually leads to higher carbon emissions than that of plant protein. To reduce carbon emissions, the partial replacement of animal protein with plant protein has attracted extensive attention; however, little is known...

  • Article
  • Open Access
60 Citations
7,334 Views
15 Pages

7 October 2020

For many years, it was believed that only amino acids, dipeptides, and tripeptides could be absorbed and thus reach the bloodstream. Nowadays, the bioavailability of oligopeptides is also considered possible, leading to new research. This pilot study...

  • Article
  • Open Access
9 Citations
3,673 Views
14 Pages

Hypotensive and Hepatoprotective Properties of the Polysaccharide-Stabilized Foaming Composition Containing Hydrolysate of Whey Proteins

  • Alexandr G. Kruchinin,
  • Olga S. Savinova,
  • Olga A. Glazunova,
  • Konstantin V. Moiseenko,
  • Evgeniya Yu. Agarkova and
  • Tatyana V. Fedorova

23 March 2021

Whey protein hydrolysates (WPHs) are one of the most promising sources of biofunctional peptides with such beneficial properties as antioxidant, antihypertensive, anti-inflammatory and others. WPHs also could be used as foaming agents for aerated pro...

  • Article
  • Open Access
3 Citations
4,455 Views
14 Pages

14 December 2022

The interactions of chicken trachea collagen hydrolysate (CTH) obtained from the enzymatic hydrolysis of by-products from a chicken slaughterhouse, with two common proteins (egg white (E) and whey (W) proteins) was studied with and without sodium chl...

  • Article
  • Open Access
5 Citations
2,288 Views
13 Pages

Reducing Effects of Whey Protein Hydrolysate on Coloration of Cured Sausages

  • Shiro Takeda,
  • Teppei Kanda,
  • Abdulatef M. Ahhmed,
  • Kazuki Sogawa,
  • Keitarou Umezu,
  • Masaya Ogata,
  • Wataru Mizunoya and
  • Ryoichi Sakata

19 December 2023

Curing produces a characteristic pink color during meat processing through the production of nitrosyl myoglobin (NOMb), which requires nitric oxide (NO). Nitrites and nitrates in coloring agents are crucial NO sources; however, a reducing agent is ne...

  • Article
  • Open Access
15 Citations
8,206 Views
13 Pages

Effects of Whey Protein or Its Hydrolysate Supplements Combined with an Energy-Restricted Diet on Weight Loss: A Randomized Controlled Trial in Older Women

  • Yue Sun,
  • Chenjie Ling,
  • Linsheng Liu,
  • Jianwei Zhang,
  • Jian Wang,
  • Xing Tong,
  • Khemayanto Hidayat,
  • Mengting Chen,
  • Xiaofang Chen and
  • Hui Zhou
  • + 4 authors

28 October 2022

An energy-restricted weight-loss approach has limitations when it used in the elderly, especially because of muscle loss. We aimed to assess the effects of whey protein (WP) or WP hydrolysate (WPH) combined with an energy-restricted diet (ERD) on wei...

  • Article
  • Open Access
1,560 Views
18 Pages

16 June 2025

Background/Objectives: Hepatic steatosis, characterized by abnormal fat accumulation in the liver, is a major health concern with limited effective treatments. Goat milk whey proteins have demonstrated various therapeutic benefits. This study aimed t...

  • Article
  • Open Access
7 Citations
3,174 Views
13 Pages

14 March 2022

The inhibition of dipeptidyl peptidase-IV (DPP-IV) and the release of glucagon-like peptide-1 (GLP-1) could normalize blood glucose levels in diabetic patients. This study evaluated the susceptibility of whey proteins to enzyme hydrolysis and the ant...

  • Feature Paper
  • Article
  • Open Access
4 Citations
2,666 Views
17 Pages

10 December 2023

In this study, the potential of whey protein hydrolysate (WPH) and treadmill exercise to prevent cognitive decline was investigated, along with their neuroprotective mechanisms. Cognitive dysfunction was induced in mice with 1 mg/kg of scopolamine, f...

  • Article
  • Open Access
10 Citations
4,705 Views
10 Pages

14 February 2022

Sarcopenia is prevalent as the aging population grows. Therefore, the need for supplements for the elderly is increasing. This study aimed to investigate the efficacy and mechanism of a Panax ginseng berry extract (GBE) and soluble whey protein hydro...

  • Article
  • Open Access
5 Citations
2,487 Views
13 Pages

Lactobacillus brevis M2-Fermented Whey Protein Hydrolysate Increases Slow-Wave Sleep via GABAA Receptors in Rodent Models

  • Hyowon Lee,
  • Hyeongyeong Kim,
  • Yeok Boo Chang,
  • Kisoo Han,
  • Hyeon-Son Choi,
  • Sung Hee Han and
  • Hyung Joo Suh

27 June 2024

In this study, we investigated the effects of whey protein hydrolysate (WPH) fermented with Lactobacillus brevis on sleep behavior and GABAergic mechanisms in rodent models. Fermentation converted the glutamate in WPH to high (3.15 ± 0.21 mg/m...

  • Communication
  • Open Access
30 Citations
4,642 Views
13 Pages

3 December 2019

Systematical consumption of functional products has a significant positive effect on health and can reduce the risk of diseases. The aim of this study was to investigate the possibility of using whey protein hydrolysate (WPH) and pumpkin pectin as in...

  • Article
  • Open Access
2 Citations
2,350 Views
12 Pages

Comparison of Oxidative and Physical Stabilities of Conjugated Linoleic Acid Emulsions Stabilized by Glycosylated Whey Protein Hydrolysates via Two Pathways

  • Meng Li,
  • Jinzhe Li,
  • Yuxuan Huang,
  • Munkh-Amgalan Gantumur,
  • Akhunzada Bilawal,
  • Abdul Qayum and
  • Zhanmei Jiang

23 June 2022

The objective of the research was to analyze and compare the oxidative and physical stabilities of conjugated linoleic acid (CLA) emulsions stabilized by two glycosylated hydrolysates (GPP-A and GPP-B) that were formed via two different pathways. Thi...

  • Proceeding Paper
  • Open Access
2,746 Views
6 Pages

Hydrolysates from a Whey Protein Concentrate Are a Promising Functional Ingredient for Diabetes Control via DPP-IV Inhibition

  • Selene Isabel Alvarez-Chávez,
  • Itzury Reyes-Ramírez,
  • Luis Guillermo González-Olivares,
  • Jesús Guadalupe Pérez-Flores,
  • Elizabeth Contreras-López,
  • Laura Berenice Olvera-Rosales,
  • Laura García-Curiel and
  • Emmanuel Pérez-Escalante

The circular economy has been a strategy for diminishing waste and improving the use of natural resources and energy in different industrial sectors. The food industry is a manufacturing and service sector with few incorporations into sustainable dev...

  • Article
  • Open Access
13 Citations
3,681 Views
17 Pages

17 October 2023

Whey protein hydrolysate (WPH) has been shown to have a variety of bioactivities. This study aimed to investigate the preventive effect of WPH on dextran sodium sulfate (DSS)-induced colitis in C57BL/6J mice. The results indicated that WPH interventi...

  • Article
  • Open Access
13 Citations
3,669 Views
17 Pages

Health Aspects, Growth Performance, and Meat Quality of Rabbits Receiving Diets Supplemented with Lettuce Fertilized with Whey Protein Hydrolysate Substituting Nitrate

  • Ali Osman,
  • Tharwat A. Imbabi,
  • Abdalla El-Hadary,
  • Islam Ibrahim Sabeq,
  • Shimaa N. Edris,
  • Abdel-Rahaman Merwad,
  • Ehab Azab,
  • Adil A. Gobouri,
  • Amaal Mohammadein and
  • Mahmoud Sitohy

Lettuce (Lactuca sativa) was grown using a foliar spray with whey protein hydrolysate (WPH) as opposed to normal nitrate fertilization. Lettuce juice was prepared from lettuce cultivated without any fertilization, nitrate fertilization, or WPH. Sixty...

  • Article
  • Open Access
7 Citations
3,097 Views
14 Pages

24 June 2023

Long-term hypercaloric intake such as a high-fat diet (HFD) could act as negative regulators on bone remodeling, thereby inducing bone loss and bone microarchitecture destruction. Currently, food-derived natural compounds represent a promising strate...

  • Article
  • Open Access
22 Citations
3,920 Views
15 Pages

The Influence of Enzymatic Hydrolysis of Whey Proteins on the Properties of Gelatin-Whey Composite Hydrogels

  • Violeta Popescu,
  • Andreia Molea,
  • Marioara Moldovan,
  • Pompilia Mioara Lopes,
  • Amalia Mazilu Moldovan and
  • George Liviu Popescu

23 June 2021

Amino-acids, peptides, and protein hydrolysates, together with their coordinating compounds, have various applications as fertilizers, nutritional supplements, additives, fillers, or active principles to produce hydrogels with therapeutic properties....

  • Article
  • Open Access
10 Citations
6,280 Views
17 Pages

14 September 2021

Background: adequate protein intake is essential to humans and, since the global demand for protein-containing foods is increasing, identifying new high-quality protein sources is needed. In this study, we investigated the acute postprandial bioavail...

  • Article
  • Open Access
6 Citations
6,680 Views
15 Pages

Whey Protein Enzymatic Breakdown: Synthesis, Analysis, and Discovery of New Biologically Active Peptides in Papain-Derived Hydrolysates

  • Michał Czelej,
  • Katarzyna Garbacz,
  • Tomasz Czernecki,
  • Kamila Rachwał,
  • Jacek Wawrzykowski and
  • Adam Waśko

25 March 2025

Bioactive peptides derived from milk proteins offer promising potential that can be unlocked through hydrolysis. Enzymatic hydrolysis is particularly noteworthy because of its mild conditions and its efficacy in producing peptides with various biolog...

  • Article
  • Open Access
20 Citations
4,114 Views
12 Pages

Production of ACE Inhibitory Peptides from Whey Proteins Modified by High Intensity Ultrasound Using Bromelain

  • Lucía Abadía-García,
  • Eduardo Castaño-Tostado,
  • Anaberta Cardador-Martínez,
  • Sandra Teresita Martín-del-Campo and
  • Silvia L. Amaya-Llano

5 September 2021

High Intensity Ultrasound (HIUS) can induce modification of the protein structure. The combination of enzymatic hydrolysis and ultrasound is an interesting strategy to improve the release of the Angiotensin-Converting Enzyme (ACE) inhibitory peptides...

  • Article
  • Open Access
6 Citations
2,536 Views
15 Pages

Peptide Mapping with LC-HRMS and In Silico Tools for Evaluation of Potential Allergenicity of Commercially Available Whey Protein Hydrolysates

  • Anastassia Taivosalo,
  • Irina Stulova,
  • Mary-Liis Kütt,
  • Tiina Kriščiunaite,
  • Taivo Lints,
  • Tatjana Gimaeva and
  • Martti Tamm

22 June 2023

Hypoallergenic formulas containing hydrolyzed protein are intended for use by infants to prevent cow’s milk protein allergy. The degree of hydrolysis of epitopic areas determines the residual allergenicity of whey protein hydrolysates (WPHs). H...

  • Article
  • Open Access
10 Citations
4,084 Views
13 Pages

15 July 2022

Tryptic WPHs with considerable residual whey protein content intact were developed from two sheep/goat WPCs (65% and 80% protein) without pH control. Pasteurization was used to avoid denaturation. Changes in non-protein nitrogen (DH_TCASN), free amin...

  • Article
  • Open Access
1 Citations
440 Views
18 Pages

4 December 2025

This study investigated the potential of Fraction I (FI, <1 kDa), a peptide fraction derived from whey protein hydrolysates (WPH) exhibiting strong in vitro scavenging activity against DPPH, superoxide, and hydroxyl radicals, to mitigate quality d...

  • Article
  • Open Access
24 Citations
7,178 Views
13 Pages

Plasma Free Amino Acid Responses to Intraduodenal Whey Protein, and Relationships with Insulin, Glucagon-Like Peptide-1 and Energy Intake in Lean Healthy Men

  • Natalie D. Luscombe-Marsh,
  • Amy T. Hutchison,
  • Stijn Soenen,
  • Robert E. Steinert,
  • Peter M. Clifton,
  • Michael Horowitz and
  • Christine Feinle-Bisset

4 January 2016

This study determined the effects of increasing loads of intraduodenal (ID) dairy protein on plasma amino acid (AA) concentrations, and their relationships with serum insulin, plasma glucagon-like peptide-1 (GLP-1) and energy intake. Sixteen healthy...

  • Article
  • Open Access
1 Citations
4,286 Views
13 Pages

Gastrointestinal Tolerance of an Infant Formula Manufactured from Extensively Hydrolysed Protein in Healthy Term Infants

  • Lindsey Otten,
  • Elisabeth Schelker,
  • Hanna Petersen,
  • Antonia Nomayo,
  • Romy Conzade,
  • Julia Günther,
  • Andrea Grieger,
  • Frank Jochum and
  • on behalf of the HASI Study Group

4 November 2023

The evaluation of secondary parameters of a prospective, randomised, controlled, multicentre intervention trial aimed to analyse gastrointestinal tolerance of an infant formula manufactured from extensively hydrolysed whey protein (eHF) compared to i...

  • Article
  • Open Access
7 Citations
3,843 Views
15 Pages

Safety and Suitability of an Infant Formula Manufactured from Extensively Hydrolysed Protein in Healthy Term Infants

  • Lindsey Otten,
  • Elisabeth Schelker,
  • Hanna Petersen,
  • Antonia Nomayo,
  • Manja Fleddermann,
  • Bianca M. Arendt,
  • Theresa Britzl,
  • Elisabeth M. Haberl and
  • Frank Jochum

14 April 2023

We aimed to demonstrate that healthy term infants experience noninferior growth with infant formula manufactured from extensively hydrolysed whey protein (eHF) compared to intact cow’s milk protein (control formula, CF). This prospective, randomised,...

  • Feature Paper
  • Article
  • Open Access
18 Citations
3,776 Views
25 Pages

Enhancing Grain Yield and Nitrogen Accumulation in Wheat Plants Grown under a Mediterranean Arid Environment by Foliar Spray with Papain-Released Whey Peptides

  • AbdAllah M. El-Sanatawy,
  • Salwa M. A. I. Ash-Shormillesy,
  • Ahmed Abou El-Yazied,
  • Hany G. Abd El-Gawad,
  • Ehab Azab,
  • Adil A. Gobouri,
  • Mahmoud Sitohy and
  • Ali Osman

24 September 2021

Due to their beneficial, stimulating impact on plant growth, productivity, and alleviating environmental stresses, protein hydrolysates have recently received increasing attention as a possible substitute. This investigation aimed to explore the effe...

  • Article
  • Open Access
1 Citations
2,311 Views
14 Pages

Impact of a Whey Protein Hydrolysate Treated by Electrodialysis with Ultrafiltration Membrane on the Development of Metabolic Syndrome and the Modulation of Gut Microbiota in Mice

  • Valentine Renaud,
  • Mélanie Faucher,
  • Marie-Julie Dubois,
  • Geneviève Pilon,
  • Thibault Varin,
  • André Marette and
  • Laurent Bazinet

19 August 2023

The development of Metabolic Syndrome (MetS) affects a large number of people around the world and represents a major issue in the field of health. Thus, it is important to implement new strategies to reduce its prevalence, and various approaches are...

  • Article
  • Open Access
2 Citations
2,721 Views
15 Pages

The probiotic foods market is expanding; however, maintaining probiotics viability is challenging during manufacturing and storage conditions. In this study, a functional ingredient containing whey protein hydrolysate-encapsulated probiotics was stan...

  • Review
  • Open Access
41 Citations
9,611 Views
25 Pages

5 June 2020

As the human body ages, skeletal muscle loses its mass and strength. It is estimated that in 10% of individuals over the age of 60, this muscle frailty has progressed to sarcopenia. Biomarkers of sarcopenia include increases in inflammatory markers a...

  • Article
  • Open Access
7 Citations
3,038 Views
14 Pages

The separation by electrodialysis with ultrafiltration membranes (EDUF), at a semi-industrial scale, of a new whey protein hydrolysate obtained from a whey protein concentrate was assessed. After 6 h of treatment, more than 9 g of peptides were recov...

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