Next Article in Journal
Nuts and Non-Alcoholic Fatty Liver Disease: Are Nuts Safe for Patients with Fatty Liver Disease?
Next Article in Special Issue
Source and Composition in Amino Acid of Dietary Proteins in the Primary Prevention and Treatment of CKD
Previous Article in Journal
Evidence Regarding Vitamin D and Risk of COVID-19 and Its Severity
Previous Article in Special Issue
Nutrient Intake and Nutritional Status in Adult Patients with Inherited Metabolic Diseases Treated with Low-Protein Diets: A Review on Urea Cycle Disorders and Branched Chain Organic Acidemias
Article

Soluble Whey Protein Hydrolysate Ameliorates Muscle Atrophy Induced by Immobilization via Regulating the PI3K/Akt Pathway in C57BL/6 Mice

1
Department of Life and Nanopharmaceutical Sciences, Graduate School, Kyung Hee University, Seoul 02447, Korea
2
Health & Nutrition R&D Group, Maeil Dairies Co., Ltd., Gyeonggi 17714, Korea
3
Neocremar R&D Center, 211, Jungdae-ro, Songpa-gu, Seoul 05702, Korea
4
Department of Preventive Pharmacy and Toxicology, College of Pharmacy, Kyung Hee University, Seoul 02447, Korea
*
Author to whom correspondence should be addressed.
Nutrients 2020, 12(11), 3362; https://doi.org/10.3390/nu12113362
Received: 9 September 2020 / Revised: 23 October 2020 / Accepted: 28 October 2020 / Published: 1 November 2020
Sarcopenia, a loss of skeletal muscle mass and function, is prevalent in older people and associated with functional decline and mortality. Protein supplementation is necessary to maintain skeletal muscle mass and whey protein hydrolysates have the best nutrient quality among food proteins. In the first study, C57BL/6 mice were subjected to immobilization for 1 week to induce muscle atrophy. Then, mice were administered with four different whey protein hydrolysates for 2 weeks with continuous immobilization. Among them, soluble whey protein hydrolysate (WP-S) had the greatest increase in grip strength, muscle weight, and cross-sectional area of muscle fiber than other whey protein hydrolysates. To investigate the molecular mechanism, we conducted another experiment with the same experimental design. WP-S significantly promoted the phosphoinositide 3-kinase (PI3K)/protein kinase B (Akt)/mammalian target of rapamycin (mTOR) pathway and inhibited the PI3K/Akt/forkhead box O (FoxO) pathway. In addition, it increased myosin heavy chain (MyHC) expression in both the soleus and quadriceps and changed MyHC isoform expressions. In conclusion, WP-S attenuated muscle atrophy induced by immobilization by enhancing the net protein content regulating muscle protein synthesis and degradation. Thus, it is a necessary and probable candidate for developing functional food to prevent sarcopenia. View Full-Text
Keywords: whey protein hydrolysate; muscle atrophy; sarcopenia; immobilization; muscle synthesis; muscle degradation; PI3K/Akt pathway whey protein hydrolysate; muscle atrophy; sarcopenia; immobilization; muscle synthesis; muscle degradation; PI3K/Akt pathway
Show Figures

Graphical abstract

MDPI and ACS Style

Shin, J.E.; Park, S.J.; Ahn, S.I.; Choung, S.-Y. Soluble Whey Protein Hydrolysate Ameliorates Muscle Atrophy Induced by Immobilization via Regulating the PI3K/Akt Pathway in C57BL/6 Mice. Nutrients 2020, 12, 3362. https://doi.org/10.3390/nu12113362

AMA Style

Shin JE, Park SJ, Ahn SI, Choung S-Y. Soluble Whey Protein Hydrolysate Ameliorates Muscle Atrophy Induced by Immobilization via Regulating the PI3K/Akt Pathway in C57BL/6 Mice. Nutrients. 2020; 12(11):3362. https://doi.org/10.3390/nu12113362

Chicago/Turabian Style

Shin, Ji E., Seok J. Park, Seung I. Ahn, and Se-Young Choung. 2020. "Soluble Whey Protein Hydrolysate Ameliorates Muscle Atrophy Induced by Immobilization via Regulating the PI3K/Akt Pathway in C57BL/6 Mice" Nutrients 12, no. 11: 3362. https://doi.org/10.3390/nu12113362

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop