This study was performed to explore the predominant responses of rumen microbiota with thymol supplementation as well as effective dose of thymol on rumen fermentation. Thymol at different concentrations, i.e., 0, 100 mg/L, 200 mg/L, and 400 mg/L (fo...
Previous studies have shown that Bacillus subtilis natto affects rumen fermentation and rumen microbial community structure, which are limited to detect a few microbial abundances using traditional methods. However, the regulation of B. subtilis natt...
Alkaline mineral complex buffer can improve rumen fermentation and affect the rumen microbiota of dairy cows. Here, we studied the effects of alkaline mineral complex buffer on serum immunity indexes, rumen fermentation and the microbiota of newborn...
This study aimed to explore the dynamic variations of rumen fermentation characteristics and bacterial community composition during a 24 h in vitro fermentation. A total of twenty-three samples were collected from original rumen fluid (ORF, n = 3), f...
Roughage particle size can influence rumen development, which is also determined by rumen microorganisms and their metabolic end-products. Therefore, the aim of this study was to evaluate the comprehensive effects of roughage length and rumen bacteri...
Methane emissions from ruminants significantly contribute to greenhouse gases. This study explores the methane mitigation effect and mechanism of S. mcclurei through in vitro rumen fermentation, aiming to establish its potential as a feed additive. W...
The objective of this experiment was to delve into the impacts of transportation on goats. Sixteen healthy goats were selected as experimental animals; these goats were transported at a speed ranging from 35 to 45 km/h for 20 h. The changes in the ph...
Microplastics (MPs) have emerged as a significant environmental threat, infiltrating livestock systems. This study presents the first in vitro investigation of the effects of low-density polyethylene (LDPE) MP contamination on rumen fermentation dyna...
Enzymes excreted by rumen microbiome facilitate the conversion of ingested plant materials into major nutrients (e.g., volatile fatty acids (VFA) and microbial proteins) required for animal growth. Diet, animal age, and health affect the structure of...
Hybridization in bovines is practiced with the main aim of improving production performance, which may imply the microbial variations in the rumen from the parental breed cross to their progeny. Besides, the interactions of offspring breed with sex i...
This study investigated the effects of replacing part of the soybean meal in the diet of Dumont lambs with urea, rapeseed meal, and cottonseed meal on their growth performance and rumen fermentation and combined rumen microbial metagenomics and metab...
This study was conducted to explore the proper time required to achieve stabilization in digestibility, serum metabolism, and rumen fermentation characteristics when different diets shift, thus providing decision-making of practical sampling frequenc...
This experiment investigated the effects of rumen-protected leucine (RP-Leu) supplementation on growth performance, rumen fermentation parameters, and rumen microbiota of fattening sheep. Forty fattening sheep (19.03 ± 0.38 kg) were randomly d...
Plectasin, a novel antimicrobial peptide, has the potential to disrupt bacterial cell walls and alter the rumen fermentation mode, making it a superior alternative to antibiotics. However, there is limited research on the effects of plectasin on rume...
The objective of this study was to investigate the effects of dietary rumen-degradable starch (RDS, g/kg of DM) to rumen-degradable protein (RDP, g/kg of DM) ratios (SPR) on in vitro rumen fermentation characteristics and microbial protein synthesis...
Milk from cows grazing on alpine pastures has higher quality than milk from indoor-fed cows, likely due to diet-driven differences in rumen microbiota. We assessed the effects of supplementing alpine herbs—each varying in its content of fiber,...
This study investigated the effects of the dietary protein level and rumen-protected methionine and lysine (RPML) on the growth performance, rumen fermentation, and serum indexes of yaks. Thirty-six male yaks were randomly assigned to a two by three...
This study aimed to investigate the effects of fumarate and nitroglycerin on rumen fermentation, methane and hydrogen production, and microbiota. In vitro rumen fermentation was used in this study with four treatment groups: control (CON), fumarate (...
Several red seaweeds have been shown to inhibit enteric CH4 production; however, the adaptation of fermentation parameters to their presence is not well understood. The objective of this study was to examine the effect of three red seaweeds (Aspararg...
This study sought to evaluate the effects of Artemisia argyi aqueous extract (AAE) on rumen fermentation parameters and the microbiota within the rumen of lambs. A total of 32 lambs that are 3 months old and 24.06 ± 0.04 kg in body weight were...
This experiment was conducted to evaluate the effects of a methionine hydroxy analog (MHA) on in vitro gas production, rumen fermentation parameters, and rumen microbiota. Two different MHA, 2-hydroxy-4-(methylthio) butanoic acid isopropyl ester (HMB...
The objective of this study was to investigate the effects of different rice varieties on in vitro rumen fermentation characteristics, microbial community, and metabolite profile of rice straw. The inbred variety Zhongjiazao 17 (Z17) and the hybrid v...
Copper is a microelement crucial for the proper functioning of animals’ metabolic processes. The function of copper in rumen fermentation processes and methanogenesis is not well analyzed. The aim of the study was to evaluate the different type...
This study evaluated the effects of sea buckthorn polysaccharides (SBP) on rumen fermentation in Hu sheep through in vitro fermentation. A single-factor randomized design was employed with four treatment groups in which SBP was added to a basal diet...
In this study, we aimed to establish the correlation between ruminal fermentation parameters and the bacterial community by comparing those of the goat, sheep, and cow, thus illustrating the main bacteria causing the difference in rumen fermentation...
Hydrogen sulfide is a Janus-faced molecule with many beneficial and toxic effects on the animal health. In ruminants, rumen fermentation plays a vital role in the digestion and absorption of nutrients. During rumen fermentation, the production of hyd...
This study evaluated the effects of selenium yeast (SY) on rumen fermentation parameters, rumen bacterial diversity, and expression pathways in goats. A total of 18 Qianbei-pockmarked weather goats from Guizhou (body weight, 25.75 ± 1.75 kg; m...
Lycium ruthenicum leaves (LRL), as an agricultural by-product rich in bioactive compounds, can be used as an unconventional feedstuff in animal diets and have the potential to improve animal health. This study investigates the effects of dietary supp...
The present study investigates the effect of 50 mL AMCB taken daily as a dietary supplement on the rumen fermentation, microbiota, and production performance of 40 Holstein dairy cows in the transition period with 2.76 ± 0.48 parity and 650 &p...
The purpose of this work was to study the dose effects of fruit oligosaccharide (FOS) supplementation on rumen fermentation parameters, methane (CH4) production and skatole production. The rumen fluid of Hu sheep was collected through their fistula a...
This study was conducted to explore the rumen fermentation characteristics, bacterial diversity, and community composition of Hu sheep under four energy provision strategies. Ninety-six Hu sheep (body weight: 17.78 ± 1.24 kg) were equally assi...
Deoxynivalenol (DON) is a type B trichothecene mycotoxin produced by Fusarium fungi. To investigate its ruminal degradability and its effect on rumen fermentation, a 2 × 5 factorial experiment was conducted in vitro with two feed substrates wit...
Dietary crude protein concentration oscillation can improve the nitrogen utilization efficiency of ruminants. However, little is known about the dynamic changes in microbiota and fermentation in the rumen of calves during the oscillation period. In t...
This study aimed to determine changes and interactions of ruminal microbiota and chemical parameters in dairy cows fed FTMR. Twelve multiparous Holstein dairy cows (Body weight = 616 ± 13.4 kg; day in milk = 106 ± 7.55 d; and parity = 2...
The present study investigated the effects of sprouted barley (SB) with different cultivation stages on fermentation characteristics and degradation kinetics in the rumen. The SB was cultivated in three different stages as follows: 0, 4, and 8 days....
This study evaluated the effects of clinoptilolite (CTL) and ferulic acid (FA) supplementation on in vitro ruminal fermentation characteristics, gas production, and bacterial abundance. Treatments were arranged in a 2 × 2 factorial design (FA:...
These experiments were conducted to evaluate the effect of excessive sulfur on rumen fermentation, microflora, and epithelial barrier function in steers through in vitro gas production and animal feeding experiments. Nine and four levels of sulfur ad...
The purpose of this study was to determine how ruminal Crabtree-negative yeast affects the nutritional characteristics of winged bean (Psophocarpus tetragonolobus) tubers (WBT), in vitro gas and digestibility, and rumen fermentation. The experiment w...
This study aimed to investigate the effects of different storage times of the mixed inoculum on in vitro rumen fermentation characteristics, microbial diversity, and community composition. The experiment was divided into five groups, with mixed inocu...
This study aimed to determine the optimal supplementation level of sodium humate (SH) for improving rumen fermentation efficiency in vitro. Using rumen fluid from four donor ewes with three experimental replicates per treatment, we evaluated a basal...
Corn, as a raw material supplying energy, plays an important role in animal husbandry. But in recent years, problems related to insufficient energy supply from corn have attracted increasing attention. Therefore, it is highly important to develop new...
Nitroethane (NE), 2-nitroethanol (NEOH), and 2-nitro-1-propanol (NPOH) were comparatively examined to determine their inhibitory actions on rumen fermentation and methanogenesis in vitro. Fermentation characteristics, CH4 and total gas production, an...
Different strategies have been used to mitigate greenhouse gas emissions from domesticated ruminants, including the removal of protozoa (defaunation). The objective of the present work was to analyze the potential of different N-oxide-containing arom...
Garlic oil (GO) and cinnamaldehyde (CIN) have shown potential to modify rumen fermentation. The aim of this study was to assess the effects of GO and CIN on rumen fermentation, microbial protein synthesis (MPS), and microbial populations in Rusitec f...
The objective of this study was to evaluate the effect of anaerobic fermentation on the in vitro ruminal production of total gas (TG), methane (CH4), carbon monoxide (CO) and hydrogen sulfide (H2S), as well as on the characteristics of ruminal fermen...
The rumen is divided into multiple rumen sacs based on anatomical structure, and each has its unique physiological environment. Tarim wapiti preserved roughage tolerance after domestication, and adaptation to the desertified environment led to the de...
The objective of this study was to evaluate the effects of potato vine and leaf mixed silage (PVS) on rumen fermentation and the microbe in ruminants and to improve the utilization of PVS resources in ruminants through in vitro gas production and fee...
This study assessed the impact of active dry yeast (ADY) on nutrient digestibility and rumen fermentation, using both in vitro and in vivo experiments with lambs. In vitro, ADYs were incubated with rumen fluid and a substrate mixture to assess gas pr...
Feeding frequency and feeding level are two important factors affecting rumen fermentation characteristics, but few studies on these have been conducted on fresh forage. Eight rumen-fistulated sheep were fed either fresh chicory or perennial ryegrass...
Twelve essential oils (EO): Anise star, cassia, geraniol, lemongrass (LEM), limonene, thyme, tea tree, coriander (COR), capsicum, black pepper, turmeric and ginger (GIN), in Experiment 1 at three doses; and different combinations of LEM, COR and GIN...
of 18