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Microorganisms, Volume 8, Issue 5

May 2020 - 171 articles

Cover Story: Filamentous bacteriophages are promising immunogenic carriers for antigen delivery. Their use is based on the modification of phage display technology; in particular, multiple copies of exogenous peptides (or polypeptides) can be covalently linked to viral capsid proteins and expressed on the phage coat. This study, for the first time, proposes the use of polymeric poly (lactic-co-glycolic acid) (PLGA) microparticles (MPs), to encapsulate fd bacteriophage displaying antigenic peptides and to demonstrate the immunogenicity of the encapsulated bacteriophages after their release by MPs. Our results show that PLGA MPs-encapsulated bacteriophages are stable and retain their immunogenic properties, demonstrating that microparticles can represent an important tool for the development of bacteriophage-based immunotherapeutic platforms. View this paper
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Articles (171)

  • Communication
  • Open Access
8 Citations
3,848 Views
8 Pages

Risk of Symptomatic Infection after Non-Primary Congenital Cytomegalovirus Infection

  • Alessandra Coscia,
  • Agata Leone,
  • Carlotta Rubino,
  • Ganna Galitska,
  • Matteo Biolatti,
  • Enrico Bertino,
  • Chiara Peila and
  • Francesco Cresi

Cytomegalovirus (CMV) is the leading cause of congenital infection. Its occurrence is phenotypically heterogeneous. The type of maternal infection, primary or non-primary, is an important factor related to the symptomatic disease, the primary infecti...

  • Opinion
  • Open Access
41 Citations
7,493 Views
12 Pages

Wine terroir is characterized by a specific taste and style influenced by the cultivar of the fermented grapes, geographical factors such as the vineyard, mesoclimate, topoclimate, and microclimate, soil geology and pedology, and the agronomic approa...

  • Article
  • Open Access
79 Citations
10,570 Views
20 Pages

Faecalibacterium prausnitzii is a major commensal bacterium in the human gut. It produces short-chain fatty acids that promote intestinal health. However, the bacterium is extremely oxygen-sensitive, making it difficult to develop as a probiotic. To...

  • Article
  • Open Access
18 Citations
4,047 Views
15 Pages

Evaluation of Indolocarbazoles from Streptomyces sanyensis as a Novel Source of Therapeutic Agents against the Brain-Eating Amoeba Naegleria fowleri

  • Aitor Rizo-Liendo,
  • Ines Sifaoui,
  • Luis Cartuche,
  • Iñigo Arberas-Jiménez,
  • María Reyes-Batlle,
  • José J. Fernández,
  • José E. Piñero,
  • Ana R. Díaz-Marrero and
  • Jacob Lorenzo-Morales

Naegleria fowleri is an opportunistic pathogenic free-living amoeba which is able to rapidly colonize the central nervous system (CNS) and causes a lethal infection known as primary amoebic meningoencephalitis (PAM). Furthermore, more than 98% of the...

  • Article
  • Open Access
10 Citations
8,316 Views
17 Pages

In the US, dried beef products (beef jerky) are a popular snack product in which the manufacture often requires the use of a heat lethality step to provide adequate reduction of pathogens of concern (i.e., 5-log reduction of Salmonella as recommended...

  • Communication
  • Open Access
15 Citations
7,148 Views
7 Pages

Key Bacteria in the Gut Microbiota Network for the Transition between Sedentary and Active Lifestyle

  • Nazareth Castellanos,
  • Gustavo G. Diez,
  • Carmen Antúnez-Almagro,
  • Carlo Bressa,
  • María Bailén,
  • Rocío González-Soltero,
  • Margarita Pérez and
  • Mar Larrosa

Physical activity modifies the gut microbiota, exerting health benefits on the host; however, the specific bacteria associated with exercise are not yet known. In this work, we propose a novel method, based on hierarchical topology, to study the diff...

  • Article
  • Open Access
29 Citations
7,188 Views
18 Pages

Actinomycetes from the Red Sea Sponge Coscinoderma mathewsi: Isolation, Diversity, and Potential for Bioactive Compounds Discovery

  • Yara I. Shamikh,
  • Aliaa A. El Shamy,
  • Yasser Gaber,
  • Usama Ramadan Abdelmohsen,
  • Hashem A. Madkour,
  • Hannes Horn,
  • Hossam M. Hassan,
  • Abeer H. Elmaidomy,
  • Dalal Hussien M. Alkhalifah and
  • Wael N. Hozzein

The diversity of actinomycetes associated with the marine sponge Coscinoderma mathewsi collected from Hurghada (Egypt) was studied. Twenty-three actinomycetes were separated and identified based on the 16S rDNA gene sequence analysis. Out of them, th...

  • Review
  • Open Access
55 Citations
8,743 Views
28 Pages

The Role of the Microbiota–Gut–Brain Axis and Antibiotics in ALS and Neurodegenerative Diseases

  • Mark Obrenovich,
  • Hayden Jaworski,
  • Tara Tadimalla,
  • Adil Mistry,
  • Lorraine Sykes,
  • George Perry and
  • Robert A. Bonomo

The human gut hosts a wide and diverse ecosystem of microorganisms termed the microbiota, which line the walls of the digestive tract and colon where they co-metabolize digestible and indigestible food to contribute a plethora of biochemical compound...

  • Article
  • Open Access
8 Citations
4,014 Views
13 Pages

Pathophysiology of Hyperechogenic Bowel in Congenitally Human Cytomegalovirus Infected Fetuses

  • Liliana Gabrielli,
  • Maria P. Bonasoni,
  • Angela Chiereghin,
  • Giulia Piccirilli,
  • Eva C. Borgatti,
  • Giuliana Simonazzi,
  • Nunzio C. M. Salfi,
  • Ione Tamagnini and
  • Tiziana Lazzarotto

Hyperechogenic bowel (HB) is a nonspecific ultrasound finding that can be associated with human cytomegalovirus (CMV) congenital infection. In this study, we investigated HB pathophysiology in CMV-infected fetuses. We examined small and large intesti...

  • Article
  • Open Access
3 Citations
3,805 Views
15 Pages

Phage Display Detection of Mimotopes that Are Shared Epitopes of Clinically and Epidemiologically Relevant Enterobacteria

  • Armando Navarro,
  • Delia Licona-Moreno,
  • Alejandro Monsalvo-Reyes,
  • Ulises Hernández-Chiñas and
  • Carlos A. Eslava-Campos

Background: Escherichia coli and Salmonella are etiologic agents of intestinal infections. A previous study showed the presence of shared epitopes between lipopolysaccharides (LPSs) of E. coli O157 and Salmonella. Aim: Using phage display, the aim of...

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Microorganisms - ISSN 2076-2607