Dual-Purpose Inoculants and Their Effects on Corn Silage
1
Division of Applied Life Science (BK21Plus, Insti. of Agri. & Life Sci.), Gyeongsang National University, Jinju 52828, Korea
2
Faculty of Animal Science, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia
3
Department of Plant Medicine, Gyeongsang National University, Jinju 52828, Korea
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Microorganisms 2020, 8(5), 765; https://doi.org/10.3390/microorganisms8050765
Received: 27 April 2020 / Revised: 15 May 2020 / Accepted: 19 May 2020 / Published: 20 May 2020
(This article belongs to the Special Issue Lactic Acid Bacteria, Biopreservation Agents for Fruit and Vegetables)
This study was conducted to screen dual-purpose lactic acid bacteria (LAB) from uncontrolled farm-scale silage, and then we confirmed their effects on corn silage. The LAB were isolated from eight farm-scale corn silages, and then we screened the antifungal activity against Fusarium graminearum and the carboxylesterase activity using spectrophotometer with p-nitrophenyl octanoate as substrate and McIlvane solution as buffer. From a total of 25 isolates, 5M2 and 6M1 isolates were selected as silage inoculants because presented both activities of antifungal and carboxylesterase. According 16S rRNA gene sequencing method, 5M2 isolate had 100.0% similarity with Lactobacillus brevis, and 6M1 isolate had 99.7% similarity with L. buchneri. Corn forage was ensiled in bale silo (500 kg) for 72 d without inoculant (CON) or with mixture of selected isolates at 1:1 ratio (INO). The INO silage had higher nutrient digestibility in the rumen than CON silage. Acetate was higher and yeasts were lower in INO silage than in CON silage on the day of silo opening. In all days of aerobic exposure, yeasts were lower in INO silage than CON silage. The present study concluded that Lactobacillus brevis 5M2 and L. buchneri 6M1 confirmed antifungal and carboxylesterase activities on farm-scale corn silage.
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Keywords:
antifungal; carboxylesterase; corn silage; lactic acid bacteria
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MDPI and ACS Style
Paradhipta, D.H.V.; Lee, S.S.; Kang, B.; Joo, Y.H.; Lee, H.J.; Lee, Y.; Kim, J.; Kim, S.C. Dual-Purpose Inoculants and Their Effects on Corn Silage. Microorganisms 2020, 8, 765. https://doi.org/10.3390/microorganisms8050765
AMA Style
Paradhipta DHV, Lee SS, Kang B, Joo YH, Lee HJ, Lee Y, Kim J, Kim SC. Dual-Purpose Inoculants and Their Effects on Corn Silage. Microorganisms. 2020; 8(5):765. https://doi.org/10.3390/microorganisms8050765
Chicago/Turabian StyleParadhipta, Dimas Hand Vidya, Seong Shin Lee, Byeongsam Kang, Young Ho Joo, Hyuk Jun Lee, Yeyeong Lee, Jinwoo Kim, and Sam Churl Kim. 2020. "Dual-Purpose Inoculants and Their Effects on Corn Silage" Microorganisms 8, no. 5: 765. https://doi.org/10.3390/microorganisms8050765
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