Next Article in Journal
Evaluation of Product Distribution in Chemostat and Batch Fermentation in Lactic Acid-Producing Komagataella phaffii Strains Utilizing Glycerol as Substrate
Previous Article in Journal
Pathophysiology of Hyperechogenic Bowel in Congenitally Human Cytomegalovirus Infected Fetuses
Previous Article in Special Issue
Recombinant Filamentous Bacteriophages Encapsulated in Biodegradable Polymeric Microparticles for Stimulation of Innate and Adaptive Immune Responses
Open AccessArticle

Phage Display Detection of Mimotopes that Are Shared Epitopes of Clinically and Epidemiologically Relevant Enterobacteria

1
Departamento de Salud Pública, Facultad de Medicina, Universidad Nacional Autónoma de México, México City CP 04510, Mexico
2
Laboratorio de Bioquímica Molecular, Unidad de Biología y Prototipos, UBIPRO, Facultad de Estudios Superiores Iztacala, Universidad Nacional Autónoma de México, Los Reyes Ixtacala Tlalnepantla, Estado de México P C 54090, Mexico
3
Unidad Periférica de Investigación Básica y Clínica en Enfermedades Infecciosas, División de Investigación de la Facultad de Medicina, UNAM: Laboratorio de Patogenicidad Bacteriana, Unidad de Hemato-Oncología e Investigación, Hospital Infantil de México Federico Gómez/Facultad de Medicina, UNAM. Dr. Márquez 162, Col. Doctores, México City CP 06720, Mexico
*
Author to whom correspondence should be addressed.
Microorganisms 2020, 8(5), 780; https://doi.org/10.3390/microorganisms8050780
Received: 10 April 2020 / Revised: 12 May 2020 / Accepted: 19 May 2020 / Published: 22 May 2020
(This article belongs to the Special Issue Phage Display—Perspectives and Translational Applications)
Background: Escherichia coli and Salmonella are etiologic agents of intestinal infections. A previous study showed the presence of shared epitopes between lipopolysaccharides (LPSs) of E. coli O157 and Salmonella. Aim: Using phage display, the aim of this study is to identify mimotopes of shared epitopes in different enterobacterial LPSs. Methods: We use anti-LPS IgG from E. coli O157 and Salmonella to select peptide mimotopes of the M13 phage. The amino acid sequence of the mimotopes is used to synthesize peptides, which are in turn used to immunize rabbits. The antibody response of the resulting sera against the LPSs and synthetic peptides (SPs) is analyzed by ELISA and by Western blot assays, indicating that LPS sites are recognized by the same antibody. In a complementary test, the reactions of human serum samples obtained from the general population against the SPs and LPSs are also analyzed. Results: From the last biopanning phase, sixty phagotopes are selected. The analysis of the peptide mimotope amino acid sequences shows that in 4 of them the S/N/A/PF motif is a common sequence. Antibodies from the sera of immunized rabbits with SP287/3, SP459/1, SP308/3, and SP073/14 react against both their own peptide and the different LPSs. The Western blot test shows a sera reaction against both the lateral chains and the cores of the LPSs. The analysis of the human sera shows a response against the SPs and LPSs. Conclusion: The designed synthetic peptides are mimotopes of LPS epitopes of Salmonella and E. coli that possess immunogenic capacity. These mimotopes could be considered for use in the design of vaccines against both enterobacteria. View Full-Text
Keywords: mimotopes; epitopes; Salmonella; E. coli; lipopolysaccharide; phagotopes; immunogenic peptides; synthetic peptides; phage display mimotopes; epitopes; Salmonella; E. coli; lipopolysaccharide; phagotopes; immunogenic peptides; synthetic peptides; phage display
Show Figures

Graphical abstract

MDPI and ACS Style

Navarro, A.; Licona-Moreno, D.; Monsalvo-Reyes, A.; Hernández-Chiñas, U.; Eslava-Campos, C.A. Phage Display Detection of Mimotopes that Are Shared Epitopes of Clinically and Epidemiologically Relevant Enterobacteria. Microorganisms 2020, 8, 780. https://doi.org/10.3390/microorganisms8050780

AMA Style

Navarro A, Licona-Moreno D, Monsalvo-Reyes A, Hernández-Chiñas U, Eslava-Campos CA. Phage Display Detection of Mimotopes that Are Shared Epitopes of Clinically and Epidemiologically Relevant Enterobacteria. Microorganisms. 2020; 8(5):780. https://doi.org/10.3390/microorganisms8050780

Chicago/Turabian Style

Navarro, Armando; Licona-Moreno, Delia; Monsalvo-Reyes, Alejandro; Hernández-Chiñas, Ulises; Eslava-Campos, Carlos A. 2020. "Phage Display Detection of Mimotopes that Are Shared Epitopes of Clinically and Epidemiologically Relevant Enterobacteria" Microorganisms 8, no. 5: 780. https://doi.org/10.3390/microorganisms8050780

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop