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Open AccessArticle

Microbial Consortiums of Hydrogenotrophic Methanogenic Mixed Cultures in Lab-Scale Ex-Situ Biogas Upgrading Systems under Different Conditions of Temperature, pH and CO

by 1, 1, 1, 2 and 1,3,*
1
The Yangtze River Water Environment Key Laboratory of the Ministry of Education, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China
2
Shanghai Key Laboratory of Atmospheric Particle Pollution and Prevention (LAP3), Department of Environmental Science and Engineering, Fudan University, Shanghai 200092, China
3
Shanghai Institute of Pollution Control and Ecological Security, Shanghai 200092, China
*
Author to whom correspondence should be addressed.
Microorganisms 2020, 8(5), 772; https://doi.org/10.3390/microorganisms8050772
Received: 6 May 2020 / Revised: 15 May 2020 / Accepted: 18 May 2020 / Published: 21 May 2020
(This article belongs to the Special Issue Anaerobic Digestion)
In this study, hydrogenotrophic methanogenic mixed cultures taken from 13 lab-scale ex-situ biogas upgrading systems under different temperature (20–70 °C), pH (6.0–8.5), and CO (0–10%, v/v) variables were systematically investigated. High-throughput 16S rRNA gene sequencing was used to identify the microbial consortia, and statistical analyses were conducted to reveal the microbial diversity, the core functional microbes, and their correlative relationships with tested variables. Overall, bacterial community was more complex than the archaea community in all mixed cultures. Hydrogenotrophic methanogens Methanothermobacter, Methanobacterium, and Methanomassiliicoccus, and putative syntrophic acetate-oxidizing bacterium Coprothermobacter and Caldanaerobacter were found to predominate, but the core functional microbes varied under different conditions. Multivariable sensitivity analysis indicated that temperature (p < 0.01) was the crucial variable to determine the microbial consortium structures in hydrogenotrophic methanogenic mixed cultures. pH (0.01 < p < 0.05) significantly interfered with the relative abundance of dominant archaea. Although CO did not affect community (p > 0.1), some potential CO-utilizing syntrophic metabolisms might be enhanced. Understanding of microbial consortia in the hydrogenotrophic methanogenic mixed cultures related to environmental variables was a great advance to reveal the microbial ecology in microbial biogas upgrading process. View Full-Text
Keywords: biogas upgrading; hydrogenotrophic methanogens; microbial community; temperature; pH; CO biogas upgrading; hydrogenotrophic methanogens; microbial community; temperature; pH; CO
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MDPI and ACS Style

Xu, J.; Bu, F.; Zhu, W.; Luo, G.; Xie, L. Microbial Consortiums of Hydrogenotrophic Methanogenic Mixed Cultures in Lab-Scale Ex-Situ Biogas Upgrading Systems under Different Conditions of Temperature, pH and CO. Microorganisms 2020, 8, 772. https://doi.org/10.3390/microorganisms8050772

AMA Style

Xu J, Bu F, Zhu W, Luo G, Xie L. Microbial Consortiums of Hydrogenotrophic Methanogenic Mixed Cultures in Lab-Scale Ex-Situ Biogas Upgrading Systems under Different Conditions of Temperature, pH and CO. Microorganisms. 2020; 8(5):772. https://doi.org/10.3390/microorganisms8050772

Chicago/Turabian Style

Xu, Jun; Bu, Fan; Zhu, Wenzhe; Luo, Gang; Xie, Li. 2020. "Microbial Consortiums of Hydrogenotrophic Methanogenic Mixed Cultures in Lab-Scale Ex-Situ Biogas Upgrading Systems under Different Conditions of Temperature, pH and CO" Microorganisms 8, no. 5: 772. https://doi.org/10.3390/microorganisms8050772

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