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Microorganisms, Volume 7, Issue 10 (October 2019)
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Cover Story (view full-size image) Longitudinal data are important to study underlying biological mechanisms and factors that [...] Read more. Longitudinal data are important to study underlying biological mechanisms and factors that influence the development of intestinal microbiota. In poultry microbiota research, chickens are often euthanized to collect intestinal samples. Fecal samples are easy to collect longitudinally but less useful to unravel functionalities of the intestinal microbiota. In this article, we compare different invasive and non-invasive sampling methods and find that cecal droppings and boot socks samples are useful alternatives for cecal samples to determine intestinal microbiota composition longitudinally. This study provides valuable knowledge of applicable tools for microbiota research. View this paper.