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Intestinal Microbiota of Grass Carp Fed Faba Beans: A Comparative Study

by Lei Zhou 1,†, Ke-tao Lin 1,†, Lian Gan 1, Ji-jia Sun 1, Chang-jun Guo 2, Li Liu 1,* and Xian-de Huang 1,*
1
Joint Laboratory of Guangdong Province and Hong Kong Region on Marine Bioresource Conservation and Exploitation, College of Marine Sciences, South China Agricultural University, Guangzhou 510642, China
2
Institute of Aquatic Economic Animals and Guangdong Province Key Laboratory for Aquatic Economic Animals, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Microorganisms 2019, 7(10), 465; https://doi.org/10.3390/microorganisms7100465
Received: 16 September 2019 / Revised: 1 October 2019 / Accepted: 16 October 2019 / Published: 17 October 2019
(This article belongs to the Section Gut Microbiota)
Many reports of the intestinal microbiota of grass carp have addressed the microbial response to diet or starvation or the effect of microbes on metabolism; however, the intestinal microbiota of crisp grass carp has yet to be elucidated. Moreover, the specific bacteria that play a role in the crispiness of grass carp fed faba beans have not been elucidated. In the present study, 16S sequencing was carried out to compare the intestinal microbiota in the fore-, mid- and hind-intestine segments of grass carp following feeding with either faba beans or formula feed. Our results showed that (1) the hind-intestine presented significant differences in diversity relative to the fore- or midintestine and (2) faba beans significantly increased the diversity of intestinal microbiota, changed the intestinal microbiota structure (Fusobacteria was reduced from 64.26% to 18.24%, while Proteobacteria was significantly increased from 17.75% to 51.99%), and decreased the metabolism of energy, cofactors and vitamins in grass carp. Furthermore, at the genus and species levels, Acinetobacter accounted for 15.09% of the microbiota, and Acinetobacter johnsonii and Acinetobacter radioresistens constituted 3.41% and 2.99%, respectively, which indicated that Acinetobacter of the family Moraxellaceae contributed to changes in the intestinal microbiota structure and could be used as a potential biomarker. These results may provide clues at the intestinal microbiota level to understanding the mechanism underlying the crispiness of grass carp fed faba beans. View Full-Text
Keywords: grass carp; faba beans; 16S sequencing; intestinal microbiota; Acinetobacter grass carp; faba beans; 16S sequencing; intestinal microbiota; Acinetobacter
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Zhou, L.; Lin, K.-T.; Gan, L.; Sun, J.-J.; Guo, C.-J.; Liu, L.; Huang, X.-D. Intestinal Microbiota of Grass Carp Fed Faba Beans: A Comparative Study. Microorganisms 2019, 7, 465.

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