Nutrients

30 pages, 4839 KiB

Open AccessArticle

Acceptability of a Colorectal Cancer-Preventive Diet Promoting Red Meat Reduction and Increased Fiber and Micronutrient Intake: A Cross-Sectional Study in Romanian Adults

by Marius-Cătălin Belean, Teodor-Andrei Maghiar, Anca-Maria Căpraru, Andreea-Adriana Neamțu, Dan Iliescu, Valentin-Cristian Iovin, Flaviu-Ionuț Faur, Meda-Ada Bugi, Alina Totorean, Sorina Tăban, Sorin Dema, Cristina-Adriana Dehelean, Bogdan Dan Totolici, Ovidiu Laurian Pop, Octavian Crețu and Carmen Neamțu

Nutrients 2025, 17(14), 2386; https://doi.org/10.3390/nu17142386 - 21 Jul 2025

Viewed by 570

Abstract

Background/Objectives: Colorectal cancer is a leading cause of cancer-related death worldwide, with rising incidence in younger adults. Unhealthy diets high in red and processed meat and low in fiber are key modifiable risk factors, highlighting the need for preventive nutritional strategies targeting [...] Read more.

Background/Objectives: Colorectal cancer is a leading cause of cancer-related death worldwide, with rising incidence in younger adults. Unhealthy diets high in red and processed meat and low in fiber are key modifiable risk factors, highlighting the need for preventive nutritional strategies targeting CRC through dietary interventions. Methods: A one-day sample diet for colorectal cancer prevention, consisting of fiber-rich meals excluding red meat and incorporating whole grains, legumes, vegetables, fruits, nuts, and lean protein alternatives (such as fish and poultry), was developed. Its acceptability was assessed in a cross-sectional study using an online questionnaire among healthy Romanian adults aged 18–50, with a total of 395 included participants. Results: Of the 395 respondents meeting the inclusion criteria (aged 18–50, no cancer or chronic gastrointestinal disorders), 63.5% were females, predominantly urban (90.1%), and highly educated. Mean age was 32.4 years; mean BMI was 25.07 kg/m². The proposed colorectal cancer-preventive diet was rated as “quite attractive” and “very attractive” by 74.9% of participants. All meals received high ratings, with dinner and the first snack being most favored. Most respondents (77.2%) found the diet satisfying and the satiety level and energy adequate, and 90.4% were willing to adopt it at least a few times per week. Financial accessibility was affirmed by 77.2% of the respondents. However, 61.8% reported difficulty eliminating red meat consumption. Female participants rated the diet significantly more attractive than males did (p = 0.041). Willingness to adopt the diet strongly correlated with higher acceptability (p < 0.0001), while BMI and education level showed no significant effect. Conclusions: The proposed colorectal cancer-preventive diet was well accepted by Romanian adults aged 18–50, with higher receptivity among women and those with higher education; willingness to adopt the diet at least a few days per week was high, especially among those psychologically ready for dietary change, while key barriers included red meat reduction and perceived cost, underscoring the need for gender-sensitive, culturally adapted interventions and further research on long-term adherence and clinical impact. Full article

(This article belongs to the Special Issue Nutrition and Dietary Guidelines for Colorectal Cancer Patients)

► Show Figures

Figure 1

17 pages, 615 KiB

Open AccessArticle

Effects of 4:3 Intermittent Fasting on Eating Behaviors and Appetite Hormones: A Secondary Analysis of a 12-Month Behavioral Weight Loss Intervention

by Matthew J. Breit, Ann E. Caldwell, Danielle M. Ostendorf, Zhaoxing Pan, Seth A. Creasy, Bryan Swanson, Kevin Clark, Emily B. Hill, Paul S. MacLean, Daniel H. Bessesen, Edward L. Melanson and Victoria A. Catenacci

Nutrients 2025, 17(14), 2385; https://doi.org/10.3390/nu17142385 - 21 Jul 2025

Viewed by 620

Abstract

Background/Objectives: Daily caloric restriction (DCR) is a common dietary weight loss strategy, but leads to metabolic and behavioral adaptations, including maladaptive eating behaviors and dysregulated appetite. Intermittent fasting (IMF) may mitigate these effects by offering diet flexibility during energy restriction. This secondary analysis [...] Read more.

Background/Objectives: Daily caloric restriction (DCR) is a common dietary weight loss strategy, but leads to metabolic and behavioral adaptations, including maladaptive eating behaviors and dysregulated appetite. Intermittent fasting (IMF) may mitigate these effects by offering diet flexibility during energy restriction. This secondary analysis compared changes in eating behaviors and appetite-related hormones between 4:3 intermittent fasting (4:3 IMF) and DCR and examined their association with weight loss over 12 months. Methods: Adults with overweight or obesity were randomized to 4:3 IMF or DCR for 12 months. Both randomized groups received a matched targeted weekly dietary energy deficit (34%), comprehensive group-based behavioral support, and a prescription to increase moderate-intensity aerobic activity to 300 min/week. Eating behaviors were assessed using validated questionnaires at baseline and months 3, 6, and 12. Fasting levels of leptin, ghrelin, peptide YY, brain-derived neurotrophic factor, and adiponectin were measured at baseline and months 6 and 12. Linear mixed models and Pearson correlations were used to evaluate outcomes. Results: Included in this analysis were 165 adults (mean ± SD; age 42 ± 9 years, BMI 34.2 ± 4.3 kg/m², 74% female) randomized to 4:3 IMF (n = 84) or DCR (n = 81). At 12 months, binge eating and uncontrolled eating scores decreased in 4:3 IMF but increased in DCR (p < 0.01 for between-group differences). Among 4:3 IMF, greater weight loss was associated with decreased uncontrolled eating (r = −0.27, p = 0.03), emotional eating (r = −0.37, p < 0.01), and increased cognitive restraint (r = 0.35, p < 0.01) at 12 months. There were no between-group differences in changes in fasting appetite-related hormones at any time point. Conclusions: Compared to DCR, 4:3 IMF exhibited improved binge eating and uncontrolled eating behaviors at 12 months. This may, in part, explain the greater weight loss achieved by 4:3 IMF versus DCR. Future studies should examine mechanisms underlying eating behavior changes with 4:3 IMF and their long-term sustainability. Full article

(This article belongs to the Special Issue Intermittent Fasting: Health Impacts and Therapeutic Potential)

► Show Figures

Graphical abstract

15 pages, 1159 KiB

Open AccessArticle

Growing Pains and Dietary Habits in Young Athletes: A Cross-Sectional Survey

by Carlos Elvira-Aranda, José Antonio Pérez-Turpin, Concepción Suárez-Llorca, Maite Pérez and Roser De-Castellar

Nutrients 2025, 17(14), 2384; https://doi.org/10.3390/nu17142384 - 21 Jul 2025

Viewed by 361

Abstract

Background/Objectives: Growing pains are a common cause of recurrent limb pain in children, but their etiology remains unclear. Physical activity and nutrition are important factors for musculoskeletal health, but their specific relationship with growing pains has not been well established in young [...] Read more.

Background/Objectives: Growing pains are a common cause of recurrent limb pain in children, but their etiology remains unclear. Physical activity and nutrition are important factors for musculoskeletal health, but their specific relationship with growing pains has not been well established in young athletes. This study aimed to assess the prevalence of growing pains in child and adolescent athletes and evaluate their adherence to the Mediterranean Diet. Methods: A cross-sectional study was conducted with 916 athletes aged 8–17 years from sports academies in Alicante, Spain. Data were collected via an online survey assessing demographics, pain types, and adherence to the Mediterranean diet. Results: Self-reported pain was highly prevalent, affecting 78.5% of children and 93.5% of adolescents. Musculoskeletal and nocturnal pain increased with age, with nocturnal pain significantly more frequent in girls among children (p < 0.001). Additionally, 32.6% of children and 51.9% of adolescents had received a formal diagnosis of growing pains. Despite this, only 13.7% reported using analgesics, with no significant gender differences. Adherence to the Mediterranean diet was mostly moderate, with children reporting higher fruit and vegetable intake than adolescents, while adolescents consumed more healthy fats and carbohydrates, and participants without pain showed overall healthier dietary patterns. Conclusions: Idiopathic musculoskeletal pain is highly prevalent among young athletes, and their adherence to a healthy diet is suboptimal, challenging the assumption that physically active children maintain well-balanced diets. This underscores the importance of early nutritional education as a strategy to support musculoskeletal health and reduce pain in physically active youth. Full article

(This article belongs to the Special Issue The Impact of Physical Activity, Lifestyle, and Nutritional Intake on Children’s Health)

► Show Figures

Figure 1

24 pages, 411 KiB

Open AccessReview

Natural Compounds and Their Potential in Eating-Related Aspects of Mental Health Disorders

by Wenbin Ma, Ralf Regenthal and Ute Krügel

Nutrients 2025, 17(14), 2383; https://doi.org/10.3390/nu17142383 - 21 Jul 2025

Viewed by 478

Abstract

Background and Objectives: Mental health and healthy eating are inextricably linked by bi-directional interaction. As pharmacological interventions for eating disorders and mental illness have limited efficacy and are associated with significant side effects, natural compounds traditionally used in these fields represent an [...] Read more.

Background and Objectives: Mental health and healthy eating are inextricably linked by bi-directional interaction. As pharmacological interventions for eating disorders and mental illness have limited efficacy and are associated with significant side effects, natural compounds traditionally used in these fields represent an extremely rich source for potential future drugs. This review aims to summarise complex and/or specific pharmacological and clinical effects of mixed compositions and individual compounds derived from Rosmarinus officinalis, Ginkgo biloba, and Bupleurum chinense as well as from Berberis vulgaris and other berberine (BBR)-containing plants, which have been traditionally used for eating and mental health purposes. Results and Conclusions: The data on favoured natural compounds and main ingredients of compound mixtures presented here could provide new impetus for preventive or targeted supplementary treatment, potential drug development, and the design of new compound congeners with improved target spectrum and potency in mental health disorders and eating-related issues. Contemporary methodological development steps in this direction are then proposed. Full article

(This article belongs to the Special Issue Eating and Mental Health Disorders)

48 pages, 888 KiB

Open AccessReview

Lifestyle Medicine for Obesity in the Era of Highly Effective Anti-Obesity Treatment

by Deepa Sannidhi, Ruth Abeles, William Andrew, Jonathan P. Bonnet, Kenneth Vitale, Varalakshmi Niranjan, Mahima Gulati, Kaitlyn Pauly, Ryan Moran, Lydia Alexander, Cassidy Le, Suraj Rajan and Camila Romero

Nutrients 2025, 17(14), 2382; https://doi.org/10.3390/nu17142382 - 21 Jul 2025

Viewed by 2514

Abstract

Despite recent advances in the treatment of obesity, lifestyle medicine remains foundational to the treatment of individuals with obesity, regardless of the modality chosen by the patient with the guidance of the clinician they are working with, including in conjunction with, as appropriate, [...] Read more.

Despite recent advances in the treatment of obesity, lifestyle medicine remains foundational to the treatment of individuals with obesity, regardless of the modality chosen by the patient with the guidance of the clinician they are working with, including in conjunction with, as appropriate, anti-obesity medications and metabolic surgery. Lifestyle medicine involves the use of diet, exercise, sleep, stress, and other lifestyle modalities in the treatment of disease. Clinicians and health systems should, after a patient-centered discussion with the patient, do their best to ensure access to lifestyle treatments. Gold standard guidelines recommend intensive, multicomponent lifestyle change programs for obesity treatments with evidence-based diet and exercise counseling and established, theoretically driven behavior change components. Clinicians treating obesity should be aware of their own biases, make efforts to reduce stigmatizing experiences in their practice, and address weight stigma in their treatment plans as needed. A variety of dietary patterns can be used to support patients with obesity, and clinicians should make evidence-based but patient-centered recommendations that aim to maximize adherence. Diet and exercise can play an important role in reducing the side effects of treatment and optimizing outcomes in weight loss, attenuating the effects of metabolic adaptation, and weight maintenance. Exercise should be increased gradually to reduce injury with a goal of 200–300 min (approximately 3.3–5 h) of moderate to vigorous intensity exercise per week to maximize weight maintenance effects with exercise prescriptions customized to patients risks. A variety of practice models can be leveraged along with the use of an interdisciplinary team to provide lifestyle medicine care for those with obesity. Full article

(This article belongs to the Special Issue The Role of Physical Activity and Diet on Weight Management)

► Show Figures

Figure 1

22 pages, 527 KiB

Open AccessArticle

Impact of Chronic Nitrate and Citrulline Malate Supplementation on Performance and Recovery in Spanish Professional Female Soccer Players: A Randomized Controlled Trial

by Marta Ramírez-Munera, Raúl Arcusa, Francisco Javier López-Román, Vicente Ávila-Gandía, Silvia Pérez-Piñero, Juan Carlos Muñoz-Carrillo, Antonio Jesús Luque-Rubia and Javier Marhuenda

Nutrients 2025, 17(14), 2381; https://doi.org/10.3390/nu17142381 - 21 Jul 2025

Viewed by 687

Abstract

Background: Pre-season training is critical for developing tolerance to high physical demands in professional soccer, and nitric oxide (NO) precursors such as dietary nitrate (NO₃⁻) and citrulline malate (CM) can support performance and recovery during this demanding phase. This [...] Read more.

Background: Pre-season training is critical for developing tolerance to high physical demands in professional soccer, and nitric oxide (NO) precursors such as dietary nitrate (NO₃⁻) and citrulline malate (CM) can support performance and recovery during this demanding phase. This study aimed to examine the effects of a four-week supplementation protocol combining 500 mg of NO₃⁻ from amaranth extract and 8 g of CM (NIT + CM) on external training load and post-match recovery in professional female soccer players during pre-season. Methods: A randomized, double-blind, placebo-controlled trial was conducted with 34 female soccer players who received either the NIT + CM product or a placebo for four weeks during pre-season. Global positioning system (GPS)-derived external load was recorded throughout the intervention. Performance tests—a countermovement jump (CMJ) test and the Wingate anaerobic test (WAnT)—and blood sampling for plasma NO₃⁻ and nitrite (NO₂⁻) concentrations were conducted at baseline and the day after a competitive match. Results: The supplementation with NIT + CM increased maximal speed (Vmax) throughout training and match play. During post-match testing, the NIT + CM group exhibited a significantly smaller decline in mean (P_mean) and minimum (P_min) power during the WAnT, along with reduced power loss in both the first (0–15 s) and second (15–30 s) intervals. Plasma NO₃⁻ concentrations significantly increased from baseline in the NIT + CM group and remained elevated 24 h after the final dose, confirming sustained systemic exposure. Conclusions: Chronic NIT + CM supplementation may enhance Vmax and help preserve anaerobic performance the day after a match. These effects could reflect improved tolerance to high training loads and sustained NO₃⁻ availability during recovery. Full article

(This article belongs to the Special Issue Nutritional Intervention and Ergogenic Aids in Sports Performance and Recovery)

► Show Figures

Graphical abstract

13 pages, 444 KiB

Open AccessReview

Key Aspects in the Nutritional Management of Polycystic Liver Disease Patients

by Saniya Khan, Simone Di Cola, Silvia Lai, Flaminia Ferri, Vincenzo Cardinale and Manuela Merli

Nutrients 2025, 17(14), 2380; https://doi.org/10.3390/nu17142380 - 21 Jul 2025

Viewed by 420

Abstract

Polycystic liver disease (PLD) is a rare genetic disorder characterized by the development of >10 fluid-filled cysts in the liver. While PLD can occur in isolation, it is most commonly associated with autosomal dominant polycystic kidney disease, adding complexity to its management. PLD [...] Read more.

Polycystic liver disease (PLD) is a rare genetic disorder characterized by the development of >10 fluid-filled cysts in the liver. While PLD can occur in isolation, it is most commonly associated with autosomal dominant polycystic kidney disease, adding complexity to its management. PLD is often asymptomatic but can lead to hepatomegaly, causing symptoms such as abdominal distension, pain and discomfort, early satiety, gastroesophageal reflux, and malnutrition, ultimately affecting patients’ quality of life. Current treatment strategies, including pharmacological and interventional approaches, focus on reducing liver volume and alleviating symptoms. However, management remains largely symptomatic, as no definitive therapies exist to halt cyst progression. Liver transplantation is the only curative option for patients with severe, progressive disease and refractory complications. The EASL guidelines recognize that PLD-related symptoms, primarily due to hepatomegaly, can contribute to involuntary weight loss and recommend assessing symptomatic patients for malnutrition and sarcopenia. Although evidence suggests that patients with PLD may be at risk of malnutrition, original data on the quality and extent of nutritional alterations remain scarce. The potential influence of nutrition on disease progression, symptom burden, and overall well-being is also largely unexplored. Given these knowledge gaps, addressing nutritional challenges, such as early satiety, is essential for optimizing symptom management and maintaining overall nutritional status. This review outlines a possible pathophysiology of malnutrition, specific dietary considerations and recommendations, and weight management in patients with PLD. Additionally, dietary complexities in patients with concurrent renal involvement are discussed, offering a practical framework for clinicians and dietitians in managing this challenging condition. Full article

(This article belongs to the Special Issue Nutrition and Frailty/Multimorbidity: Preventive and Clinical Nutritional Management)

► Show Figures

Figure 1

21 pages, 2952 KiB

Open AccessArticle

Beverage-Specific Modulation of Urinary Inflammatory Biomarkers After Endurance Running in Trained Males

by Katsuhiko Suzuki, Kazue Kanda and Sihui Ma

Nutrients 2025, 17(14), 2379; https://doi.org/10.3390/nu17142379 - 21 Jul 2025

Viewed by 368

Abstract

Background: The differential effects of post-exercise rehydration beverages on inflammatory processes and organ protection remain incompletely characterized. This study investigated how beverages with distinct compositions influence urinary biomarkers following endurance exercise. Methods: In a randomized crossover design, eight trained male runners performed 6000 [...] Read more.

Background: The differential effects of post-exercise rehydration beverages on inflammatory processes and organ protection remain incompletely characterized. This study investigated how beverages with distinct compositions influence urinary biomarkers following endurance exercise. Methods: In a randomized crossover design, eight trained male runners performed 6000 m pace running followed by consumption of 500 mL of either: water (Drink 1), hypotonic sports drink (Drink 2, 200 mOsm/L), oral rehydration solution (Drink 3, 270 mOsm/L), or modified hypotonic formulation (Drink 4, 200 mOsm/L). After 60 min, participants completed a 1000 m time trial. Urine samples were collected at baseline, post-6000 m, and post-1000 m for analysis of biochemical parameters and inflammatory cytokines. Results: No significant differences in 1000 m performance were observed between trials. Drink 3 significantly reduced creatinine and uric acid excretion compared to other beverages (p < 0.05), suggesting decreased waste product elimination. Creatinine-corrected intestinal fatty acid-binding protein values were lower with Drinks 2 and 3, indicating potential intestinal protection. Notably, Drink 4 showed modest but significant enhancement of IL-4 excretion (p < 0.05, ηp² = 0.347), demonstrating beverage-specific modulation of anti-inflammatory cytokines with moderate effect sizes. Conclusions: Different beverage formulations exert distinct effects on waste product elimination, intestinal organ damage markers, and inflammatory cytokine profiles. These findings suggest that beverage selection should be tailored to specific recovery priorities and training contexts. Full article

(This article belongs to the Special Issue Performance in Sports, Physical Activity, Exercise: Nutrition, Fatigue and Recovery)

► Show Figures

Figure 1

5 pages, 162 KiB

Open AccessEditorial

Advances in Infant and Pediatric Feeding and Nutrition

by Jann Foster

Nutrients 2025, 17(14), 2378; https://doi.org/10.3390/nu17142378 - 21 Jul 2025

Viewed by 340

Abstract

This Special Issue presents original works and reviews that delve into how specific feeding strategies, spanning from lactation to complementary feeding in infancy, impact growth and neurofunctional development [...] Full article

(This article belongs to the Special Issue Advances in Infant and Pediatric Feeding and Nutrition)

17 pages, 582 KiB

Open AccessArticle

Dietary and Genetic Aspects of Polycystic Ovary Syndrome (PCOS) in Polish Women—Part I: Nutritional Status and Dietary Intake

by Karolina Nowosad, Małgorzata Ostrowska, Paweł Glibowski, Katarzyna Iłowiecka and Wojciech Koch

Nutrients 2025, 17(14), 2377; https://doi.org/10.3390/nu17142377 - 21 Jul 2025

Cited by 1 | Viewed by 807

Abstract

Background: Polycystic ovary syndrome (PCOS) is a common endocrine and metabolic disorder characterized by reproductive and metabolic abnormality disorders. Dietary factors influence the body composition and hydration status, which may exacerbate PCOS symptoms. The aim of this study was to assess the associations [...] Read more.

Background: Polycystic ovary syndrome (PCOS) is a common endocrine and metabolic disorder characterized by reproductive and metabolic abnormality disorders. Dietary factors influence the body composition and hydration status, which may exacerbate PCOS symptoms. The aim of this study was to assess the associations between the habitual nutrient intake and bioelectrical impedance analysis parameters in Polish women with PCOS and healthy controls, in order to identify potential nutritional targets for a non-pharmacological intervention. Methods: This study involved 50 women aged 18–45 years (25 with PCOS and 25 healthy). Participants kept 7-day food diaries and their body composition was assessed using the SECA mBCA 515 analyzer. The nutrient intake was compared with EFSA recommendations. Results: Women with PCOS had a higher body weight, waist circumference and body mass index, visceral adipose tissue, and fat mass index, despite no difference in their total energy intake. They consumed more omega-3 fatty acids (EPA + DHA) than the control group. Vitamin D deficiency and irregular supplementation were common in both groups. Body composition parameters such as the phase angle and ECW/TBW ratio correlated with the diet quality—especially with protein; fiber; and vitamin B2, B12, and folate levels. Conclusions: The obtained results showed significant differences in body compositions and the presence of a relationship between the nutrient intake and bioimpedance parameters in women with PCOS. These results emphasize the importance of a comprehensive nutritional and body composition assessment in planning dietary interventions in this group of patients. Full article

(This article belongs to the Section Nutrition in Women)

► Show Figures

Figure 1

2 pages, 144 KiB

Open AccessReply

Reply to Trexler, E.T. Common Errors in Sports Nutrition Meta-Analyses Lead to Distortion of Pooled Effect Estimates. Comment on “Viribay et al. Effects of Arginine Supplementation on Athletic Performance Based on Energy Metabolism: A Systematic Review and Meta-Analysis. Nutrients 2020, 12, 1300”

by Aitor Viribay, Julen Fernández-Landa, Jesús Seco-Calvo and Juan Mielgo-Ayuso

Nutrients 2025, 17(14), 2376; https://doi.org/10.3390/nu17142376 - 21 Jul 2025

Viewed by 234

Abstract

We deeply appreciate the time and effort invested by the comment author [...] Full article

4 pages, 329 KiB

Open AccessComment

Common Errors in Sports Nutrition Meta-Analyses Lead to Distortion of Pooled Effect Estimates. Comment on Viribay et al. Effects of Arginine Supplementation on Athletic Performance Based on Energy Metabolism: A Systematic Review and Meta-Analysis. Nutrients 2020, 12, 1300

by Eric T. Trexler

Nutrients 2025, 17(14), 2375; https://doi.org/10.3390/nu17142375 - 21 Jul 2025

Viewed by 348

Abstract

Viribay and colleagues published a meta-analysis called “Effects of Arginine Supplementation on Athletic Performance Based on Energy Metabolism: A Systematic Review and Meta-Analysis” in Nutrients in May of 2020. This meta-analysis sought to quantify the effects of arginine supplementation on aerobic and anaerobic [...] Read more.

Viribay and colleagues published a meta-analysis called “Effects of Arginine Supplementation on Athletic Performance Based on Energy Metabolism: A Systematic Review and Meta-Analysis” in Nutrients in May of 2020. This meta-analysis sought to quantify the effects of arginine supplementation on aerobic and anaerobic performance outcomes. In the course of conducting this analysis, the researchers made multiple errors that are common among meta-analyses in the field of sports nutrition. This comment discusses how these issues impact the results and interpretation of the paper, and how individuals who are interpreting or conducting sports nutrition meta-analyses in the future can identify or avoid similar errors. Full article

► Show Figures

Figure 1

15 pages, 470 KiB

Open AccessReview

The Visceral Adiposity Index and Its Usefulness in the Prediction of Cardiometabolic Disorders

by Grzegorz K. Jakubiak, Georgian Badicu, Stanisław Surma, Ewa Waluga-Kozłowska, Artur Chwalba and Natalia Pawlas

Nutrients 2025, 17(14), 2374; https://doi.org/10.3390/nu17142374 - 20 Jul 2025

Viewed by 704

Abstract

Obesity is currently one of the most critical public health problems. Although there is no doubt that obesity is a significant risk factor for developing metabolic disorders, this relationship is not completely straightforward. On the one hand, some patients affected by obesity are [...] Read more.

Obesity is currently one of the most critical public health problems. Although there is no doubt that obesity is a significant risk factor for developing metabolic disorders, this relationship is not completely straightforward. On the one hand, some patients affected by obesity are metabolically unhealthy, while others are metabolically healthy; on the other hand, metabolic syndrome (MetS) can also occur in people with a normal body weight. A commonly used tool for diagnosing obesity is the body mass index (BMI), but the search for better anthropometric measures is ongoing due to the significant limitations of this measure. Obesity can lead to MetS and cardiovascular diseases (CVDs). Adipose tissue dysfunction is the fundamental mechanism linking obesity and cardiometabolic diseases, which is rooted in the disturbed secretion of adipokines. The visceral adiposity index (VAI) is calculated based on the BMI, waist circumference (WC), blood triglycerides (TG), and high-density lipoprotein cholesterol (HDL-C) concentrations. It was proposed in 2010 by Amato et al. as a parameter indicating adipose tissue dysfunction and cardiometabolic risk. According to the research conducted so far, some data confirm a relationship between the VAI value and the risk of developing prediabetes, diabetes, insulin resistance, fatty liver disease, MetS, CVD, and chronic kidney disease. Further research is needed to support the implementation of VAI assessment in routine clinical practice. The purpose of this paper is to present the results of a narrative literature review summarizing current knowledge regarding the VAI and its usefulness in clinical practice for assessing cardiometabolic risk. Full article

(This article belongs to the Special Issue Nutritional and Hormonal Pathways in Metabolic Disease)

► Show Figures

Figure 1

23 pages, 739 KiB

Open AccessReview

Dietary Nitrogen and Its Role in the Gut Microbiome and Inflammatory Bowel Disease: A Narrative Review

by Matthew Herrera and Lauri O. Byerley

Nutrients 2025, 17(14), 2373; https://doi.org/10.3390/nu17142373 - 20 Jul 2025

Cited by 1 | Viewed by 685

Abstract

In recent years, gut microbiota has emerged as a critical regulator of gastrointestinal health and disease, with its role in inflammatory bowel disease (IBD)—including Crohn’s disease and ulcerative colitis—being particularly significant. Among the many factors influencing the gut microbiota, dietary components such as [...] Read more.

In recent years, gut microbiota has emerged as a critical regulator of gastrointestinal health and disease, with its role in inflammatory bowel disease (IBD)—including Crohn’s disease and ulcerative colitis—being particularly significant. Among the many factors influencing the gut microbiota, dietary components such as fibers, fats, and polyphenols have received substantial attention. However, nitrogen-containing compounds, such as amino acids, nitrates, urea, and even nucleic acids, such as purines, remain underexplored despite their integral role in shaping microbial ecology, host metabolism, and immune responses. Some of these compounds are metabolized by gut bacteria into bioactive molecules such as short-chain fatty acids, ammonia, and nitric oxide, which exert diverse effects on mucosal integrity and inflammation. IBD pathophysiology is characterized by chronic inflammation, microbial dysbiosis, and compromised epithelial barriers. Nitrogen metabolism contributes significantly to these processes by influencing microbial composition, metabolite production, and host immune pathways. The breakdown of various nitrogen-containing compounds in the body leads to the production of byproducts, such as ammonia and hydrogen sulfide, which have been implicated in mucosal damage and immune dysregulation. At the same time, nitrogen-derived molecules, such as short-chain fatty acids and nitric oxide, exhibit protective effects, underscoring the dual role of dietary nitrogen in health and disease. This narrative review highlights the complex interactions between dietary nitrogen sources, gut microbiota, and IBD pathogenesis. We summarize the mechanisms by which nitrogen compounds influence microbial dynamics, identify their contributions to inflammation and barrier dysfunction, and explore their therapeutic potential. Multidisciplinary approaches integrating clinical, metabolomic, and microbiome research are essential to unravel the full scope of nitrogen’s role in gut health and identify novel therapeutic targets. Full article

(This article belongs to the Special Issue Diet–Microbiome Interaction in Gastrointestinal Disorders)

► Show Figures

Figure 1

12 pages, 1261 KiB

Open AccessArticle

Sex Differences in the Association Between the Korean Healthy Eating Index and Liver Enzymes Among Korean Adults

by Seong-Uk Baek and Jin-Ha Yoon

Nutrients 2025, 17(14), 2372; https://doi.org/10.3390/nu17142372 - 20 Jul 2025

Viewed by 27844

Abstract

Background/Objectives: Dietary quality plays a crucial role in maintaining liver function. In this study, we examined sex differences in the association between dietary quality and elevated liver enzyme levels in Korean adults. Methods: This study included a nationwide sample of 15,997 males and [...] Read more.

Background/Objectives: Dietary quality plays a crucial role in maintaining liver function. In this study, we examined sex differences in the association between dietary quality and elevated liver enzyme levels in Korean adults. Methods: This study included a nationwide sample of 15,997 males and 22,300 females in South Korea. Dietary assessment was performed using the Korean Healthy Eating Index (KHEI), an evidence-based dietary quality index that quantitatively reflects adherence to Korean dietary guidelines (range 0–100). Serum levels of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) were measured, with individuals classified as having elevated AST or ALT levels when values were ≥40 IU/L or ≥35 IU/L, respectively. Logistic regression analysis was performed to examine the association between the KHEI and elevated AST or ALT levels stratified by sex. Odds ratios (ORs) and 95% confidence intervals (CIs) were determined. Results: Among the males, a 10-point increase in the KHEI score was inversely associated with the likelihood of having elevated AST (OR: 0.90; 95% CI: 0.85–0.96) and ALT (OR: 0.96; 95% CI: 0.92–1.00) levels, respectively. Among the females, there was no clear association between the KHEI and elevated AST (OR: 0.98; 95% CI: 0.91–1.05) or ALT (OR: 1.00; 95% CI: 0.95–1.05) levels. Conclusion: Further research is warranted to elucidate the underlying mechanisms of the observed sex-specific associations and guide the development of targeted dietary interventions for liver health in males and females. Full article

(This article belongs to the Special Issue Nutritional Support for Improving the Life Quality in Liver Disease)

► Show Figures

Figure 1

17 pages, 1774 KiB

Open AccessArticle

A Randomized Double-Blind Trial of the Effect of Liupao Tea on Metabolic Parameters, Body Composition, and Gut Microbiota in Adults with Metabolic Syndrome

by Yuyang Wang, Qiang Hu, Qiliu Jiang, Jiamin Jiang, Biandi Li and Defu Ma

Nutrients 2025, 17(14), 2371; https://doi.org/10.3390/nu17142371 - 19 Jul 2025

Viewed by 639

Abstract

Background: Metabolic syndrome (MetS) represents a significant global health challenge. Liupao tea (LPT), a post-fermented dark tea, has shown potential metabolic benefits, but clinical evidence remains limited. Objectives: This study aimed to investigate the effects of LPT with varying aging durations [...] Read more.

Background: Metabolic syndrome (MetS) represents a significant global health challenge. Liupao tea (LPT), a post-fermented dark tea, has shown potential metabolic benefits, but clinical evidence remains limited. Objectives: This study aimed to investigate the effects of LPT with varying aging durations on clinical parameters, body composition and gut microbiota in individuals with MetS. Methods: In a randomized, double-blind trial, patients with MetS were randomly assigned to intervention groups, receiving 6 g/day of LPT aged for 1, 4, 7, or 10 years, respectively, over a 90-day intervention period. Blood pressure, lipid and glucose levels, body weight, body composition, and gut microbiota were assessed at baseline and post-intervention. Results: A total of 71 participants, with a mean age of 53.5 years, were included. At the final assessment, significant reductions in both systolic and diastolic blood pressure were observed in the 10-year-aged groups (p < 0.05). In terms of lipid profiles, the 1-year-aged group showed a significant decrease in total cholesterol (TC), while low-density lipoprotein cholesterol (LDL-C) levels significantly decreased in the 1-, 4-, 7-, and 10-year-aged groups (p < 0.05). All intervention groups showed significant reductions in body weight, body fat mass (BFM), along with an increase in muscle mass (MM) (p < 0.05). A decrease in the Firmicutes/Bacteroides (F/B) ratio was observed in the 10-year-aged group. No significant differences in clinical parameters or body composition regulation were observed between groups with varying aging durations (p > 0.05). Conclusions: LPT intervention effectively improves metabolic health and modulates gut microbiota in MetS patients, irrespective of aging duration. These findings support LPT as a functional beverage for the management of MetS. Full article

(This article belongs to the Special Issue Nutritional Strategies in the Prevention and Treatment of Metabolic Syndrome (2nd Edition))

► Show Figures

Figure 1

27 pages, 2460 KiB

Open AccessArticle

Lifestyle and Perceived Well-Being in Children and Teens: Importance of Exercise and Sedentary Behavior

by Nadia Solaro, Gianluigi Oggionni, Giuseppina Bernardelli, Mara Malacarne, Eleonora Pagani, Mariacarla Ferrari, Gianfranco Parati and Daniela Lucini

Nutrients 2025, 17(14), 2370; https://doi.org/10.3390/nu17142370 - 19 Jul 2025

Viewed by 380

Abstract

Background/Objectives: Childhood/youth are ideally the best periods to teach healthy behaviors; unfortunately, children/adolescents are frequently characterized by unhealthy lifestyles and reduced well-being. Lifestyle improvement early in life may play a fundamental role in determining present health, preventing many chronic diseases, and fostering [...] Read more.

Background/Objectives: Childhood/youth are ideally the best periods to teach healthy behaviors; unfortunately, children/adolescents are frequently characterized by unhealthy lifestyles and reduced well-being. Lifestyle improvement early in life may play a fundamental role in determining present health, preventing many chronic diseases, and fostering well-being. Having a clear picture of the lifestyle characteristics of a group can help institutions and schools define effective educational and intervention strategies. This observational study aims to examine whether information collected from children and adolescents about their lifestyles and perceptions of well-being could help identify particular groups that deserve specific interventions, implemented by the school, to improve their overall health. Methods: After administering a simple lifestyle/well-being questionnaire to 225 children/adolescents at an Italian school complex, we investigated the relationships between lifestyles and perceptions of well-being by constructing statistical indicators through nonlinear principal component analysis. Then, we defined lifestyle typologies based on lifestyle indicators and studied the distribution of the well-being indicator across such typologies, also adjusting for sex and age effects. Results: The study shows that lifestyle worsens with age (p < 0.001) and influences overall well-being perception. We identified four lifestyle typologies by combining two indicators of sedentary behavior and sleep, and of quality of nutrition and the volume of physical activity. The healthier the lifestyle, the better the overall well-being perception is (represented by the indicator that includes the perceived quality of health, sleep, and academic performance) (p = 0.005). Conclusions: Tailored educational/intervention strategies that consider specific groups’ characteristics, rather than general counseling, might be more effective at improving health/well-being. Full article

(This article belongs to the Special Issue Health Promotion in School-Aged Children: Lifestyle Factors, Interventions, and Health Outcomes)

► Show Figures

Graphical abstract

25 pages, 811 KiB

Open AccessArticle

Vegetarianísh—How “Flexitarian” Eating Patterns Are Defined and Their Role in Global Food-Based Dietary Guidance

by Julie M. Hess, Kaden Robinson and Angela J. Scheett

Nutrients 2025, 17(14), 2369; https://doi.org/10.3390/nu17142369 - 19 Jul 2025

Viewed by 627

Abstract

Background/Objectives: A dietary pattern that simply reduces animal-based foods may be more acceptable to consumers than strict vegetarian or vegan diets. The objective of this investigation was to identify the most consistently used definitions of “flexitarian” dietary patterns, or dietary patterns with a [...] Read more.

Background/Objectives: A dietary pattern that simply reduces animal-based foods may be more acceptable to consumers than strict vegetarian or vegan diets. The objective of this investigation was to identify the most consistently used definitions of “flexitarian” dietary patterns, or dietary patterns with a reduced amount of animal foods. Then, sets of food-based dietary guidance (FBDG) from different countries and regions were evaluated to determine whether their guidance could accommodate flexitarian diets. Methods: Literature searches yielded 86 total results on flexitarian eating after screening by title/abstract, full text availability, and English language. Definitions of “flexitarian” were extracted from each article then reviewed and summarized. FBDGs available in English were downloaded from the Food and Agriculture Organization of the United Nations website. Guidance related to reduced animal product diets was extracted from FBDGs for eating patterns closest to 2000 kcal. Results: The summary definition of flexitarian included eating at least one animal product (dairy, eggs, meat, or fish) at least once per month but less than once per week. FBDGs from n = 42 countries or regions were downloaded and data extracted. Only FBDG from Sri Lanka explicitly describe a “semi-vegetarian” eating pattern, though n = 12 FBDGs describe a vegetarian pattern and n = 14 recommend reducing meat or animal food and/or choosing meat/dairy alternatives. Conclusions: Following a flexitarian dietary pattern in terms of reducing or limiting red meat is feasible and even implicitly recommended by the official dietary guidance of several countries. Most FBDGs examined did not include recommendations to decrease dairy or fish intake. Full article

(This article belongs to the Special Issue Towards Healthy and Sustainable Diets: Environmental and Nutritional Perspectives)

► Show Figures

Figure 1

17 pages, 1471 KiB

Open AccessArticle

American Basil, Ocimum americanum, Has Neuroprotective Properties in the Aging Process

by Ionara Rodrigues Siqueira, Cláudia Vanzella, Gisele Agustini Lovatel, Karine Bertoldi, Christiano Spindler, Felipe dos Santos Moysés, Adriana Vizuete, Gilsane Lino von Poser and Carlos Alexandre Netto

Nutrients 2025, 17(14), 2368; https://doi.org/10.3390/nu17142368 - 19 Jul 2025

Viewed by 713

Abstract

Background/Objectives: There is evidence concerning herbal medicines and plant-based compounds, including Lamiaceae species, as putative senolytic agents; however, there are only a few reports on Ocimum americanum properties using rat models. The aim of this study was to investigate the neuroprotective effects [...] Read more.

Background/Objectives: There is evidence concerning herbal medicines and plant-based compounds, including Lamiaceae species, as putative senolytic agents; however, there are only a few reports on Ocimum americanum properties using rat models. The aim of this study was to investigate the neuroprotective effects and potential modes of action of Ocimum americanum L. using ex vivo and in vivo assays to assess the effects of OAEE on hippocampal tissue from young adult and late middle-aged Wistar rats, with a focus on oxidative stress, cholinesterase activity, and neuroinflammatory markers. Methods: Ocimum americanum ethanol extract (OAEE) was incubated with hippocampal slices of young adult and late middle-aged male Wistar rats exposed to H₂O₂; an acute treatment with OAEE was evaluated in aversive memory performance and neurochemical parameters, such as hippocampal cellular oxidative state, and anticholinesterase activity, and a diet supplementation of OAEE were evaluated on several hippocampal biochemical parameters, such as oxidative state, anticholinesterase activity, and neuroinflammatory parameters in young adult and late middle-aged male rats. Results: OAEE reversed the H₂O₂-induced impaired cellular viability in hippocampal slices from young adult rats, as well as protected hippocampal slices against H₂O₂-induced damage in both young adult and late middle-aged Wistar rats, indicating its neuroprotective action. Chronic dietary OAEE supplementation reduced aging-induced increases in reactive species and lipid peroxidation levels in the hippocampus. Indeed, this supplementation reduced the TNF-α content in hippocampus from both ages, and IL-1β levels in young adult rats. Conclusions: The antioxidant actions of OAEE here observed, preventing the lipoperoxidation, as well as its anti-neuroinflammatory effect, might be related to neuroprotective effect. Our findings add evidence to support the idea of the potential use of Ocimum americanum as a nutraceutical or functional food in the aging process. Full article

(This article belongs to the Special Issue Functional Foods and Sustainable Health (2nd Edition))

► Show Figures

Figure 1

21 pages, 2139 KiB

Open AccessReview

Orchestrating Nutrient Homeostasis: RNA-Binding Proteins as Molecular Conductors in Metabolic Disease Pathogenesis

by Siyuan Sun, Xinchun Li, Jianan Zhai, Chenxu Lu, Weiru Yu, Wenhao Wu and Juan Chen

Nutrients 2025, 17(14), 2367; https://doi.org/10.3390/nu17142367 - 19 Jul 2025

Viewed by 508

Abstract

RNA-binding proteins (RBPs) are critical regulators of post-transcriptional processes, playing essential roles in nutrient metabolism and metabolic homeostasis. This literature review explores how RBPs influence the metabolism of glucose, lipid, and amino acid metabolism by controlling processes like mRNA stability and translation regulation. [...] Read more.

RNA-binding proteins (RBPs) are critical regulators of post-transcriptional processes, playing essential roles in nutrient metabolism and metabolic homeostasis. This literature review explores how RBPs influence the metabolism of glucose, lipid, and amino acid metabolism by controlling processes like mRNA stability and translation regulation. The dysregulation of RBPs, including HuR, PTB, and YTHDF1, is linked to metabolic diseases such as obesity, diabetes, and non-alcoholic fatty liver disease. Advances in techniques like TREX technology and transcriptome analysis have deepened our understanding of RBP functions. Additionally, RBPs show promise as potential biomarkers and targets for new therapies. Future research directions in RBPs could focus on tissue-specific regulation and nutrient–RBP interactions. This could pave the way for more personalized treatments and improved metabolic health. Full article

(This article belongs to the Special Issue Diet and Nutrition: Metabolic Diseases---2nd Edition)

► Show Figures

Figure 1

13 pages, 506 KiB

Open AccessArticle

The Impact of Tea Consumption on Prediabetes Regression and Progression: A Prospective Cohort Study

by Tingting Li, Christopher K. Rayner, Michael Horowitz, Karen Jones, Cong Xie, Weikun Huang, Zilin Sun, Shanhu Qiu and Tongzhi Wu

Nutrients 2025, 17(14), 2366; https://doi.org/10.3390/nu17142366 - 19 Jul 2025

Viewed by 585

Abstract

Background: Lifestyle modifications are pivotal to preventing the progression of prediabetes and associated cardiometabolic diseases. Recent evidence from cross-sectional analysis of community-dwelling Chinese adults suggests that regular consumption of tea, particularly dark tea, is associated with a reduced risk of both prediabetes and [...] Read more.

Background: Lifestyle modifications are pivotal to preventing the progression of prediabetes and associated cardiometabolic diseases. Recent evidence from cross-sectional analysis of community-dwelling Chinese adults suggests that regular consumption of tea, particularly dark tea, is associated with a reduced risk of both prediabetes and type 2 diabetes. However, the effects of tea consumption on prediabetes progression and regression remain uncertain. This study investigated the associations of tea consumption with prediabetes progression and regression in Chinese adults with prediabetes. Methods: A cohort of 2662 Chinese adults with prediabetes was followed over ~3 years. Baseline tea consumption, including the type (green, black, dark, or other) and frequency (daily, sometimes, or nil), was assessed using standardized questionnaires. Prediabetes was defined according to the American Diabetes Association criteria. Multinomial logistic and linear regression analyses with multivariable adjustment was performed to evaluate associations. Results: Compared to non-tea drinkers, dark tea consumers were less likely to progress to type 2 diabetes (odds ratio [OR]: 0.28, 95% confidence interval [CI]: 0.11, 0.72, p = 0.01), whereas green tea consumption was associated with a reduced probability of regressing to normoglycemia (OR: 0.73, 95 CI%: 0.59, 0.90, p = 0.01). Conclusions: These findings support further exploration of dark tea consumption as a strategy to reduce prediabetes progression, and suggest that effects of green tea consumption should also be examined more closely in this population. Full article

(This article belongs to the Section Nutrition and Diabetes)

► Show Figures

Figure 1

22 pages, 3024 KiB

Open AccessArticle

Effects of Ginger Supplementation on Markers of Inflammation and Functional Capacity in Individuals with Mild to Moderate Joint Pain

by Jacob Broeckel, Landry Estes, Megan Leonard, Broderick L. Dickerson, Drew E. Gonzalez, Martin Purpura, Ralf Jäger, Ryan J. Sowinski, Christopher J. Rasmussen and Richard B. Kreider

Nutrients 2025, 17(14), 2365; https://doi.org/10.3390/nu17142365 - 18 Jul 2025

Viewed by 1815

Abstract

Background: Ginger contains gingerols, shagaols, paradols, gingerdiones, and terpenes, which have been shown to display anti-inflammatory properties and inhibit pain receptors. For this reason, ginger has been marketed as a natural analgesic. This study examined whether a specialized ginger extract obtained through supercritical [...] Read more.

Background: Ginger contains gingerols, shagaols, paradols, gingerdiones, and terpenes, which have been shown to display anti-inflammatory properties and inhibit pain receptors. For this reason, ginger has been marketed as a natural analgesic. This study examined whether a specialized ginger extract obtained through supercritical CO₂ extraction and subsequent fermentation affects pain perception, functional capacity, and markers of inflammation. Methods: Thirty men and women (56.0 ± 9.0 years, 164.4 ± 14 cm, 86.5 ± 20.9 kg, 31.0 ± 7.5 kg/m²) with a history of mild to severe joint and muscle pain as well as inflammation participated in a placebo-controlled, randomized, parallel-arm study. Participants donated fasting blood, completed questionnaires, rated pain in the thighs to standardized pressure, and then completed squats/deep knee bends, while holding 30% of body mass, for 3 sets of 10 repetitions on days 0, 30, and 56 of supplementation. Participants repeated tests after 2 days of recovery following each testing session. Participants were matched by demographics and randomized to ingest 125 mg/d of a placebo or ginger (standardized to contain 10% total gingerols and no more than 3% total shogaols) for 58 days. Data were analyzed by a general linear model (GLM) analysis of variance with repeated measures, mean changes from the baseline with 95% confidence intervals, and chi-squared analysis. Results: There was evidence that ginger supplementation attenuated perceptions of muscle pain in the vastus medialis; improved ratings of pain, stiffness, and functional capacity; and affected several inflammatory markers (e.g., IL-6, INF-ϒ, TNF-α, and C-Reactive Protein concentrations), particularly following two days of recovery from resistance exercise. There was also evidence that ginger supplementation increased eosinophils and was associated with less frequent but not significantly different use of over-the-counter analgesics. Conclusions: Ginger supplementation (125 mg/d, providing 12.5 mg/d of gingerols) appears to have some favorable effects on perceptions of pain, functional capacity, and inflammatory markers in men and women experiencing mild to moderate muscle and joint pain. Registered clinical trial #ISRCTN74292348. Full article

(This article belongs to the Section Phytochemicals and Human Health)

► Show Figures

Figure 1

5 pages, 157 KiB

Open AccessEditorial

Mediterranean Diet and Metabolic Syndrome

by Antonia Giacco, Federica Cioffi and Elena Silvestri

Nutrients 2025, 17(14), 2364; https://doi.org/10.3390/nu17142364 - 18 Jul 2025

Viewed by 496

Abstract

The Mediterranean diet (MD), a nutritional model rooted in the traditional dietary patterns of Southern Europe and first defined by the pioneering work of Ancel Keys [...] Full article

(This article belongs to the Special Issue Mediterranean Diet and Metabolic Syndrome)

17 pages, 7840 KiB

Open AccessArticle

Systemic and Retinal Protective Effects of Butyrate in Early Type 2 Diabetes via Gut Microbiota–Lipid Metabolism Interaction

by Haijun Gong, Haoyu Zuo, Keling Wu, Xinbo Gao, Yuqing Lan and Ling Zhao

Nutrients 2025, 17(14), 2363; https://doi.org/10.3390/nu17142363 - 18 Jul 2025

Viewed by 449

Abstract

Background: Early neurovascular unit (NVU) impairment plays a critical role in the pathogenesis of diabetic retinopathy (DR), often preceding clinically detectable changes. Butyrate, a short-chain fatty acid (SCFA) derived from gut microbiota, has shown promising metabolic and anti-inflammatory effects. Methods: This study [...] Read more.

Background: Early neurovascular unit (NVU) impairment plays a critical role in the pathogenesis of diabetic retinopathy (DR), often preceding clinically detectable changes. Butyrate, a short-chain fatty acid (SCFA) derived from gut microbiota, has shown promising metabolic and anti-inflammatory effects. Methods: This study investigated the protective potential of oral butyrate supplementation in a mouse model of early type 2 diabetes mellitus (T2DM) induced by a high-fat diet and streptozotocin. Mice (C57BL/6J) received sodium butyrate (5 g/L in drinking water) for 12 weeks. Retinal NVU integrity was assessed using widefield swept-source optical coherence tomography angiography (WF SS-OCTA), alongside evaluations of systemic glucose and lipid metabolism, hepatic steatosis, visual function, and gut microbiota composition via 16S rRNA sequencing. Results: Butyrate supplementation significantly reduced body weight, fasting glucose, serum cholesterol, and hepatic lipid accumulation. Microbiome analysis demonstrated a partial reversal of gut dysbiosis, characterized by increased SCFA-producing taxa (Ruminococcaceae, Oscillibacter, Lachnospiraceae) and decreased pro-inflammatory, lipid-metabolism-related genera (Rikenella, Ileibacterium). KEGG pathway analysis further revealed enrichment in microbial lipid metabolism functions (fabG, ABC.CD.A, and transketolase). Retinal vascular and neurodegenerative alterations—including reduced vessel density and retinal thinning—were markedly attenuated by butyrate, as revealed by WF SS-OCTA. OKN testing indicated partial improvement in visual function, despite unchanged ERG amplitudes. Conclusions: Butyrate supplementation mitigates early NVU damage in the diabetic retina by improving glucose and lipid metabolism and partially restoring gut microbial balance. This study also underscores the utility of WF SS-OCTA as a powerful noninvasive tool for detecting early neurovascular changes in DR. Full article

(This article belongs to the Section Nutrition and Diabetes)

► Show Figures

Figure 1

23 pages, 885 KiB

Open AccessReview

Thyroid Health and Selenium: The Critical Role of Adequate Intake from Fetal Development to Adolescence

by Valeria Calcaterra, Hellas Cena, Ilaria Anna Maria Scavone, Ilaria Zambon, Silvia Taranto, Cecilia Ricciardi Rizzo, Chiara Ferrara, Marianna Diotti and Gianvincenzo Zuccotti

Nutrients 2025, 17(14), 2362; https://doi.org/10.3390/nu17142362 - 18 Jul 2025

Viewed by 749

Abstract

The thyroid gland plays a crucial role in regulating metabolism and supporting development through the production of the hormones T4 and T3. These hormones are essential during childhood for nervous system myelination, physical growth, puberty, skeletal and dental maturation, and overall metabolic balance. [...] Read more.

The thyroid gland plays a crucial role in regulating metabolism and supporting development through the production of the hormones T4 and T3. These hormones are essential during childhood for nervous system myelination, physical growth, puberty, skeletal and dental maturation, and overall metabolic balance. In early infancy, when the hypothalamic–pituitary–thyroid axis is still immature, thyroid dysfunction can result in a range of long-term complications. The metabolism and action of thyroid hormones depend not only on iodine but also on other vital micronutrients, particularly selenium (Se). This narrative review aims to comprehensively examine the role of selenium in maintaining thyroid health from fetal life through adolescence. Selenium is a key micronutrient involved in thyroid development, hormone synthesis, antioxidant defense, and immune regulation, especially during pregnancy and childhood. Inadequate selenium levels may contribute to the onset, progression, and clinical management of various thyroid disorders, particularly hypothyroidism and autoimmune thyroid diseases. Although scientific evidence supports selenium’s critical functions in hormone metabolism and antioxidant protection, public awareness and monitoring of selenium intake remain insufficient. Beyond the need for further research, there is an urgent call for integrated public health strategies, ranging from sustainable, food-based approaches to targeted clinical screening and educational programs. Promoting awareness of selenium’s importance and incorporating selenium status into maternal and pediatric care protocols could play a significant role in preventing deficiencies and supporting long-term endocrine and neurodevelopmental health. Full article

(This article belongs to the Special Issue Nutrition in the Early Years: Feeding Challenges and Their Health Implications)

► Show Figures

Figure 1

16 pages, 391 KiB

Open AccessSystematic Review

High-Protein Dietary Interventions in Heart Failure: A Systematic Review of Clinical and Functional Outcomes

by Lorraine S. Evangelista, Rebecca Meraz, Kelly L. Wierenga, Angelina P. Nguyen, Alona D. Angosta and Jennifer Kawi

Nutrients 2025, 17(14), 2361; https://doi.org/10.3390/nu17142361 - 18 Jul 2025

Viewed by 539

Abstract

Background: Heart failure (HF) is frequently associated with skeletal muscle wasting, reduced functional capacity, and malnutrition. High-protein diets offer a promising nutritional intervention to improve these outcomes in individuals with HF. Objective: This systematic review evaluated randomized controlled trials of high-protein dietary interventions [...] Read more.

Background: Heart failure (HF) is frequently associated with skeletal muscle wasting, reduced functional capacity, and malnutrition. High-protein diets offer a promising nutritional intervention to improve these outcomes in individuals with HF. Objective: This systematic review evaluated randomized controlled trials of high-protein dietary interventions in HF populations, with emphasis on intervention characteristics, quantitative benefits, and risk of bias. Methods: We conducted a comprehensive search in PubMed, MEDLINE, Embase, and Cochrane CENTRAL from inception to June 2025. Eligible studies enrolled adults (≥18 years) with HF, implemented high-protein regimens (≥1.1 g/kg/day or ~25–30% of energy), and reported on functional capacity, body composition, muscle strength, clinical outcomes, or biochemical markers. Two reviewers independently screened, extracted data, and assessed bias (Cochrane RoB 2). Heterogeneity in dosing, duration, and outcomes precluded meta-analysis; we therefore provide a narrative synthesis. Results: Ten trials (nine randomized controlled trials, one pilot) involving 1080 patients (median n = 38; range 21–652) were included. High-protein interventions yielded mean improvements in six-minute walk distance of +32 ± 14 m, lean body mass gain of +1.6 ± 0.9 kg, and 9 ± 4% enhancement in quality-of-life scores; muscle strength effects varied from −2% to +11%. Two studies reported an 18% reduction in HF readmissions (p < 0.05). The risk-of-bias assessment identified two low-risk, three moderate-risk, and one high-risk study. Key limitations include small sample sizes, varied protein dosing (1.1–1.5 g/kg/day), short follow-up (2–6 months), and outcome heterogeneity. Conclusions: High-protein dietary strategies appear to confer modest, clinically relevant gains in functional capacity, nutritional status, and HF readmission risk. Larger, well-powered trials with standardized dosing and longer follow-up are necessary to establish optimal protein targets, long-term efficacy, and safety. Full article

(This article belongs to the Special Issue Dietary and Exercise Interventions to Target Obesity and Chronic Diseases)

► Show Figures

Figure 1

17 pages, 1694 KiB

Open AccessArticle

Gut Microbiota Shifts After a Weight Loss Program in Adults with Obesity: The WLM3P Study

by Vanessa Pereira, Amanda Cuevas-Sierra, Victor de la O, Rita Salvado, Inês Barreiros-Mota, Inês Castela, Alexandra Camelo, Inês Brandão, Christophe Espírito Santo, Ana Faria, Conceição Calhau, Marta P. Silvestre and André Moreira-Rosário

Nutrients 2025, 17(14), 2360; https://doi.org/10.3390/nu17142360 - 18 Jul 2025

Viewed by 552

Abstract

Background: The gut microbiota is increasingly recognized as a key modulator in obesity management, influencing host energy balance, lipid metabolism, and inflammatory pathways. With obesity prevalence continuing to rise globally, dietary interventions that promote beneficial microbial shifts are essential for enhancing weight loss [...] Read more.

Background: The gut microbiota is increasingly recognized as a key modulator in obesity management, influencing host energy balance, lipid metabolism, and inflammatory pathways. With obesity prevalence continuing to rise globally, dietary interventions that promote beneficial microbial shifts are essential for enhancing weight loss outcomes and long-term health. Objective: This study investigated the effects of the multicomponent Weight Loss Maintenance 3 Phases Program (WLM3P), which integrates caloric restriction, a high-protein low-carbohydrate diet, time-restricted eating (10h TRE), dietary supplementation (prebiotics and phytochemicals), and digital app-based support on gut microbiota composition compared to a standard low-carbohydrate diet (LCD) in adults with obesity. The analysis focused exclusively on the 6-month weight loss period corresponding to Phases 1 and 2 of the WLM3P intervention. Methods: In this sub-analysis of a randomized controlled trial (ClinicalTrials.gov Identifier: NCT04192357), 58 adults with obesity (BMI 30.0–39.9 kg/m²) were randomized to the WLM3P (n = 29) or LCD (n = 29) groups. Stool samples were collected at baseline and 6 months for 16S rRNA sequencing. Alpha and beta diversity were assessed, and genus-level differential abundance was determined using EdgeR and LEfSe. Associations between microbial taxa and clinical outcomes were evaluated using regression models. Results: After 6-month, the WLM3P group showed a significant increase in alpha diversity (p = 0.03) and a significant change in beta diversity (p < 0.01), while no significant changes were observed in the LCD group. Differential abundance analysis revealed specific microbial signatures in WLM3P participants, including increased levels of Faecalibacterium. Notably, higher Faecalibacterium abundance was associated with greater reductions in fat mass (kg, %) and visceral adiposity (cm²) in the WLM3P group compared to LCD (p < 0.01). Conclusions: These findings suggest a potential microbiota-mediated mechanism in weight loss, where Faecalibacterium may enhance fat reduction effectiveness in the context of the WLM3P intervention. Full article

(This article belongs to the Section Prebiotics and Probiotics)

► Show Figures

Figure 1

17 pages, 264 KiB

Open AccessArticle

Donate or Not to Donate—Willingness to Donate and Accept Donor Human Milk

by Yael Lahav and Elad Harison

Nutrients 2025, 17(14), 2359; https://doi.org/10.3390/nu17142359 - 18 Jul 2025

Viewed by 427

Abstract

Background/Objectives: Breast milk donation is increasingly recognized as a vital public health resource, providing optimal nutrition for infants who cannot be breastfed by their biological mothers. Human milk banks play a crucial role in supporting infant health, particularly for preterm and medically [...] Read more.

Background/Objectives: Breast milk donation is increasingly recognized as a vital public health resource, providing optimal nutrition for infants who cannot be breastfed by their biological mothers. Human milk banks play a crucial role in supporting infant health, particularly for preterm and medically vulnerable infants. Despite its recognized benefits, the practice of breast milk donation and utilization remains influenced by a complex interplay of individual, social, and cultural factors. Methods: This study systematically examines how demographic and sociocultural variables are associated with attitudes and behaviors related to breast milk donation. Data were collected from 1223 Israeli mothers via questionnaires distributed through WhatsApp groups between April and May 2025. By analyzing the questionnaire results, the research identified key predictors of willingness to donate and use donated milk, as well as patterns of awareness and perceived support within different population groups. Results: Higher education (χ² = 12.87, p = 0.0016) and settlement type (χ² = 83.096, p = 0.000) were significantly associated with greater willingness to donate breast milk. Income level had no effect on donation behavior, though higher-income participants were more open to its use (χ² = 86.838, p = 0.000). Lifestyle also influenced perceptions of social support (F(2, 1220) = 259.4036, p < 0.001) and cultural support (F(2, 1220) = 601.2383, p < 0.001) of milk donation and use. Significant correlation was found between perceived cultural and social support (t = 5.140, p = 0.000), emphasizing their interrelated influence on milk donation attitudes. Conclusions: The findings assist in guiding the development of public health policies, as well as targeted awareness campaigns, to promote equitable access to donor milk services across varied communities. From the public health perspective, the findings of this study can orientate campaigns that encourage both donation and use of breast milk focusing on segments of the population in which the level of awareness is relatively low. Full article

(This article belongs to the Special Issue Maternal Diet, Body Composition and Offspring Health)

19 pages, 2565 KiB

Open AccessArticle

Anti-Thrombotic Effects of Coprinus comatus Fibrinolytic Enzyme in Zebrafish

by Yan Jing, Jinyu Wang, Yating He, Zedan Liu and Xiaolan Liu

Nutrients 2025, 17(14), 2358; https://doi.org/10.3390/nu17142358 - 18 Jul 2025

Viewed by 321

Abstract

Objectives: This study investigated the antithrombotic properties of a fibrinolytic enzyme (CFE) purified from the culture supernatant of Coprinus comatus using a zebrafish thrombosis model. Methods: A phenylhydrazine-induced thrombosis model was employed to evaluate the in vivo thrombolytic efficacy and mechanisms of CFE. [...] Read more.

Objectives: This study investigated the antithrombotic properties of a fibrinolytic enzyme (CFE) purified from the culture supernatant of Coprinus comatus using a zebrafish thrombosis model. Methods: A phenylhydrazine-induced thrombosis model was employed to evaluate the in vivo thrombolytic efficacy and mechanisms of CFE. Results: CFE significantly attenuated thrombogenesis by inhibiting erythrocyte aggregation in the caudal vessels, reducing staining intensity (3.61-fold decrease) and staining area (3.89-fold decrease). Concurrently, CFE enhanced cardiac hemodynamics, increasing erythrocyte staining intensity (9.29-fold) and staining area (5.55-fold) while achieving an 85.19% thrombosis inhibition rate. Behavioral analysis confirmed improved motility, with CFE-treated zebrafish exhibiting 2.23-fold increases in total movement distance and average speed, alongside a 3.59-fold extension in active movement duration. Mechanistically, ELISA revealed the multi-pathway activity of CFE, promoting fibrinolysis through reductions in plasminogen, fibrinogen, and D-dimer; inhibiting platelet activation via downregulation of prostaglandin-endoperoxide synthase (PTGS), thromboxane A2 (TXA2), P-selectin, and von Willebrand factor (vWF); and modulating coagulation cascades through elevated protein C and tissue factor pathway inhibitor (TFPI) with concurrent suppression of coagulation factor VII (FVII). Conclusions: These results indicate that the fibrinolytic enzyme CFE, derived from Coprinus comatus, exerts potent antithrombotic effects, supporting its potential as a basis for fungal-derived natural antithrombotic functional food ingredients. Full article

(This article belongs to the Section Clinical Nutrition)

► Show Figures

Figure 1

23 pages, 1809 KiB

Open AccessReview

Pediatric Familial Hypercholesterolemia: Targeting Intestinal Absorption and Other Therapeutic Strategies

by Konstantinos Arvanitakis, Elena Chatzikalil, Christina Antza, Christos Topalidis, Georgios Kalopitas, Elena Solomou, Vasilios Kotsis, Georgios Germanidis, Theocharis Koufakis and Michael Doumas

Nutrients 2025, 17(14), 2357; https://doi.org/10.3390/nu17142357 - 18 Jul 2025

Viewed by 1312

Abstract

Familial hypercholesterolemia (FH) is a genetic disorder marked by significantly elevated levels of low-density lipoprotein cholesterol (LDL-C) since childhood, substantially increasing the risk of premature atherosclerosis and cardiovascular disease. While dysfunction of hepatic LDL-C receptors is the main underlying cause, the gastrointestinal tract [...] Read more.

Familial hypercholesterolemia (FH) is a genetic disorder marked by significantly elevated levels of low-density lipoprotein cholesterol (LDL-C) since childhood, substantially increasing the risk of premature atherosclerosis and cardiovascular disease. While dysfunction of hepatic LDL-C receptors is the main underlying cause, the gastrointestinal tract plays a key role in cholesterol homeostasis and represents an important therapeutic target. Inhibition of intestinal cholesterol absorption has emerged as an effective strategy in the management of pediatric FH, particularly in patients for whom statins may not be the ideal first-line treatment. Ezetimibe, an inhibitor of the Niemann-Pick C1-like 1 (NPC1L1) protein, has been shown to reduce LDL-C levels in children with FH, with a greater efficacy observed when used in combination with statins. Bile acid sequestrants also enhance cholesterol excretion but are often limited by gastrointestinal side effects, while dietary interventions, such as phytosterol supplementation and fiber-enriched diets, provide additional benefits in lowering LDL-C and are generally well tolerated. Emerging therapies, including microbiota-targeted strategies and novel cholesterol absorption inhibitors, show promise for expanding future treatment options. This review explores the mechanisms of intestinal cholesterol absorption and their relevance to pediatric FH. We examine key pathways, including dietary cholesterol uptake through NPC1L1, bile acid reabsorption, and cholesterol efflux mediated by ATP-binding cassette transporters, while also discussing clinical and experimental evidence on pharmacological and dietary interventions that modulate these pathways. A deeper understanding of cholesterol metabolism, the emerging role of the gut microbiota, and innovative therapeutic agents can support the development of more effective and personalized approaches to the treatment of children with FH. Full article

(This article belongs to the Special Issue Nutrition, Lifestyle and Treatment in Patients with Hypercholesterolemia)

► Show Figures

Figure 1

Journal Menu

Journal Browser

Nutrients, Volume 17, Issue 14 (July-2 2025) – 134 articles

Further Information

Guidelines

MDPI Initiatives

Follow MDPI