Nutrients

16 pages, 1275 KiB

Open AccessEditor’s ChoiceArticle

From Mother–Fetus Dyad to Mother–Milk–Infant Triad: Sex Differences in Macronutrient Composition of Breast Milk

by Maria Lithoxopoulou, Calliope Karastogiannidou, Anastasia Karagkiozi, Iliani Eleni Zafeiriadou, Ekaterini Pilati, Elisavet Diamanti, Stavros Kalogiannis and Emilia Vassilopoulou

Nutrients 2025, 17(9), 1422; https://doi.org/10.3390/nu17091422 - 23 Apr 2025

Viewed by 17611

Abstract

Background: The composition of breast milk is influenced by a variety of factors, including maternal anthropometric characteristics, dietary and lifestyle habits, and lactation and feeding parameters. Emerging evidence also suggests that an infant’s sex may play a role in shaping breast milk composition. [...] Read more.

Background: The composition of breast milk is influenced by a variety of factors, including maternal anthropometric characteristics, dietary and lifestyle habits, and lactation and feeding parameters. Emerging evidence also suggests that an infant’s sex may play a role in shaping breast milk composition. Objective: This study aims to investigate the macronutrient composition of early breast milk up to 3 months postpartum, with a particular focus on potential differences related to the infant’s sex. Methods: A total of 102 breast milk samples were collected at four time points across three lactation stages, representing colostrum, transitional, and mature milk, from a cohort consisting of 51 consenting mothers of Mediterranean origin who met the inclusion criteria. The samples were analyzed using mid-infrared spectroscopy to determine their macronutrient composition. Results: Colostrum from mothers of male infants contained approximately 60% higher levels of nitrogenous compounds, crude protein, true protein, and non-protein nitrogen compared to colostrum from mothers of female infants. Transitional milk for female infants contained higher fat, total solids, and energy. No significant differences in macronutrient composition were observed in mature milk between the sexes. For both sexes, colostrum contained more nitrogenous compounds and fewer total solids than mature milk. Male colostrum had lower carbohydrate levels compared to the later stages of lactation, while female samples showed no significant changes. Strong positive correlations between fat and crude protein were found for both sexes during the transitional and mature milk stages. In male colostrum, all macronutrients were interrelated. Conclusions: The observed differences in the composition of colostrum and transitional breast milk for male and female neonates suggest potential sex-specific nutritional adaptations during early lactation. These findings may have implications for personalized infant nutrition strategies in cases where breast feeding is hampered, as well as for understanding early neonatal adaptations. Full article

(This article belongs to the Special Issue Interactions Between Early Nutrition, Early Environment, and Immunity)

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17 pages, 1054 KiB

Open AccessEditor’s ChoiceArticle

Does Poultry Consumption Increase the Risk of Mortality for Gastrointestinal Cancers? A Preliminary Competing Risk Analysis

by Caterina Bonfiglio, Rossella Tatoli, Rossella Donghia, Pasqua Letizia Pesole and Gianluigi Giannelli

Nutrients 2025, 17(8), 1370; https://doi.org/10.3390/nu17081370 - 17 Apr 2025

Viewed by 66792

Abstract

Background: Poultry meat is currently among the most widely consumed meats in Italy and worldwide. Poultry is reasonably affordable and accessible, explaining the high global consumption rates. This population-based prospective cohort study investigated the association between meat consumption and gastrointestinal cancers (GCs) [...] Read more.

Background: Poultry meat is currently among the most widely consumed meats in Italy and worldwide. Poultry is reasonably affordable and accessible, explaining the high global consumption rates. This population-based prospective cohort study investigated the association between meat consumption and gastrointestinal cancers (GCs) and other causes of mortality in southern Italy. Methods: Data were collected from 4869 participants in the MICOL and NUTRIHEP cohorts. The EPIC questionnaire was used to elicit information on food and drink consumption. For analytical purposes, weekly meat consumption was grouped into four categories: total meat: <200 g, 201–300 g, 301–400 g, and >400 g red meat: <150 g, 150–250 g, 251–350 g, and >350 g; poultry: <100 g, 100–200 g, 201–300 g, and >300 g. Cox proportional hazard regression and competing risk models were employed for statistical analysis. Results: Analyzing weekly poultry consumption, it was observed that subjects consuming more than 300 g had a 27% higher risk of death from all causes [HR 1.27; 95% CI (1.00; 1.61)] than those consuming less than 100 g. In addition, for GCs, the SHR for weekly poultry consumption above 300 g was 2.27 [95% CI (1.23; 4.17)], a risk that for men increased to 2.61 [95% CI (1.31; 5.19)]. Conclusions: Our study showed that poultry consumption above 300 g/week is associated with a statistically significant increased mortality risk both from all causes and from GCs. The risk is higher for men than for women. Full article

(This article belongs to the Special Issue Nutritional Value of Meat and Meat Products and Their Role in Human Health—3rd Edition)

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31 pages, 793 KiB

Open AccessEditor’s ChoiceReview

Vitamin D and Colorectal Cancer Prevention: Immunological Mechanisms, Inflammatory Pathways, and Nutritional Implications

by Mónika Fekete, Andrea Lehoczki, Ágnes Szappanos, Virág Zábó, Csilla Kaposvári, Alpár Horváth, Árpád Farkas, Vince Fazekas-Pongor, Dávid Major, Ágnes Lipécz, Tamás Csípő and János Tamás Varga

Nutrients 2025, 17(8), 1351; https://doi.org/10.3390/nu17081351 - 15 Apr 2025

Cited by 5 | Viewed by 9732

Abstract

Vitamin D plays a crucial role in the regulation of the immune system, with immunomodulatory effects that are key in the prevention of colorectal cancer (CRC). Over the past decades, research has shown that this steroid hormone impacts much more than bone health, [...] Read more.

Vitamin D plays a crucial role in the regulation of the immune system, with immunomodulatory effects that are key in the prevention of colorectal cancer (CRC). Over the past decades, research has shown that this steroid hormone impacts much more than bone health, significantly influencing immune responses. Vitamin D enhances immune organ functions such as the spleen and lymph nodes, and boosts T-cell activity, which is essential in defending the body against tumors. Additionally, vitamin D mitigates inflammatory responses closely linked to cancer development, reducing the inflammation that contributes to CRC. It acts via vitamin D receptors (VDRs) expressed on immune cells, modulating immune responses. Adequate vitamin D levels influence gene expression related to inflammation and cell proliferation, inhibiting tumor development. Vitamin D also activates mechanisms that suppress cancer cell survival, proliferation, migration, and metastasis. Low levels of vitamin D have been associated with an increased risk of CRC, with deficiency correlating with higher disease incidence. Lifestyle factors, such as a diet high in red meat and calories but low in fiber, fruits, and vegetables, as well as physical inactivity, contribute significantly to CRC risk. Insufficient calcium and vitamin D intake are also linked to disease occurrence and poorer clinical outcomes. Maintaining optimal vitamin D levels and adequate dietary intake is crucial in preventing CRC and improving patient prognosis. This review explores the role of vitamin D in immune regulation and summarizes findings from randomized clinical trials assessing the effects of vitamin D supplementation on CRC outcomes. Full article

(This article belongs to the Special Issue Dose–Response Relationships of Vitamin D Status and Vitamin D Intake with Health Outcomes—2nd Edition)

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13 pages, 609 KiB

Open AccessEditor’s ChoiceArticle

Effects of Pomegranate Extract on Inflammatory Markers and Cardiometabolic Risk Factors in Adults Aged 55–70 Years: A Randomised Controlled Parallel Trial

by Grace Farhat, Jhama Malla, Jay Vadher and Emad A. S. Al-Dujaili

Nutrients 2025, 17(7), 1235; https://doi.org/10.3390/nu17071235 - 1 Apr 2025

Cited by 1 | Viewed by 5565

Abstract

Background: Chronic inflammation increases morbidity in older adults and significantly impacts healthy ageing. Pomegranate extract (PE), rich in polyphenols, has been suggested to reduce inflammation and could prevent cardiovascular disease. However, there is limited research examining the potential of PE in disease [...] Read more.

Background: Chronic inflammation increases morbidity in older adults and significantly impacts healthy ageing. Pomegranate extract (PE), rich in polyphenols, has been suggested to reduce inflammation and could prevent cardiovascular disease. However, there is limited research examining the potential of PE in disease prevention in ageing. Methods: A two-arm double-blind parallel trial was conducted, in which participants received either placebo capsules (maltodextrin) or pomegranate extract (740 mg) daily for 12 weeks. At baseline, week 6, and week 12, anthropometric measurements, blood pressure, and blood samples were collected. Serum inflammatory markers (IL-6, IL-1-α, IL1-β, IL-2, TNF-α, CRP and PAI-1), fasting blood glucose, and lipid levels were also measured. Results: A total of 86 participants met the eligibility criteria, with 76 completing the trial. A significant interaction between treatment and time was observed for the IL-6 (p = 0.02) and IL1-β (p = 0.05) levels, with both parameters significantly decreasing in the PE group. CRP and TNF-α showed a downward trend in the PE group, but it was not statistically significant (p > 0.05). Systolic blood pressure significantly decreased in the PE group (by 5.22 ± 1.26 mmHg (SE), p = 0.04), indicating potential clinical relevance, with diastolic blood pressure showing a similar downward trend (2.94 ± 1.08 mmHg (SE), p = 0.3). Despite being apparently healthy with no diagnosed diseases, a substantial number of participants exhibited elevated levels of inflammatory markers and systolic blood pressure. Conclusions: PE can lower inflammatory markers and blood pressure, which can be high in both normal-weight and overweight older adults, making it a cost-effective measure to promote healthy ageing. Further long-term studies are needed to address the limitations of this 3-month study, including the overrepresentation of normal-weight participants, and to gain a better understanding of the impact of weight on the above-mentioned outcomes. Full article

(This article belongs to the Special Issue The Role of Healthy Eating and Physical Activity in Longevity)

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22 pages, 884 KiB

Open AccessEditor’s ChoiceReview

The Gut–Brain–Microbiota Connection and Its Role in Autism Spectrum Disorders

by Ewelina Młynarska, Ewelina Barszcz, Emilian Budny, Agata Gajewska, Kacper Kopeć, Jakub Wasiak, Jacek Rysz and Beata Franczyk

Nutrients 2025, 17(7), 1135; https://doi.org/10.3390/nu17071135 - 25 Mar 2025

Cited by 3 | Viewed by 7136

Abstract

Autism spectrum disorder (ASD) is a group of complex neurodevelopmental conditions with a heterogeneous and multifactorial etiology that is not yet fully understood. Among the various factors that may contribute to ASD development, alterations in the gut microbiota have been increasingly recognized. Microorganisms [...] Read more.

Autism spectrum disorder (ASD) is a group of complex neurodevelopmental conditions with a heterogeneous and multifactorial etiology that is not yet fully understood. Among the various factors that may contribute to ASD development, alterations in the gut microbiota have been increasingly recognized. Microorganisms in the gastrointestinal tract play a crucial role in the gut–brain axis (GBA), affecting nervous system development and behavior. Dysbiosis, or an imbalance in the microbiota, has been linked to both behavioral and gastrointestinal (GI) symptoms in individuals with ASD. The microbiota interacts with the central nervous system through mechanisms such as the production of short-chain fatty acids (SCFAs), the regulation of neurotransmitters, and immune system modulation. Alterations in its composition, including reduced diversity or an overabundance of specific bacterial taxa, have been associated with the severity of ASD symptoms. Dietary modifications, such as gluten-free or antioxidant-rich diets, have shown potential for improving gut health and alleviating behavioral symptoms. Probiotics, with their anti-inflammatory properties, may support neural health and reduce neuroinflammation. Fecal microbiota transplantation (FMT) is being considered, particularly for individuals with persistent GI symptoms. It has shown promising outcomes in enhancing microbial diversity and mitigating GI and behavioral symptoms. However, its limitations should be considered, as discussed in this narrative review. Further research is essential to better understand the long-term effects and safety of these therapies. Emphasizing the importance of patient stratification and phenotype characterization is crucial for developing personalized treatment strategies that account for individual microbiota profiles, genetic predispositions, and coexisting conditions. This approach could lead to more effective interventions for individuals with ASD. Recent findings suggest that gut microbiota may play a key role in innovative therapeutic approaches to ASD management. Full article

(This article belongs to the Special Issue Pathways Linking Nutrition with Cognitive and Mental Health)

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16 pages, 800 KiB

Open AccessEditor’s ChoiceArticle

Influence of Intermittent Fasting on Body Composition, Physical Performance, and the Orexinergic System in Postmenopausal Women: A Pilot Study

by Anna A. Valenzano, Paride Vasco, Gabriella D’Orsi, Raffaella R. R. Marzovillo, Maria Torquato, Giovanni Messina, Rita Polito and Giuseppe Cibelli

Nutrients 2025, 17(7), 1121; https://doi.org/10.3390/nu17071121 - 24 Mar 2025

Viewed by 6253

Abstract

Objective: This study aims to evaluate the effects of different nutritional strategies, specifically intermittent fasting (IF) combined with high-intensity interval training (HIIT) versus a low-calorie diet (LCD), on body composition, physical performance, and the orexinergic system in postmenopausal women. Methods: A [...] Read more.

Objective: This study aims to evaluate the effects of different nutritional strategies, specifically intermittent fasting (IF) combined with high-intensity interval training (HIIT) versus a low-calorie diet (LCD), on body composition, physical performance, and the orexinergic system in postmenopausal women. Methods: A randomized controlled trial involving thirty postmenopausal women (mean age 57.50 ± 6.50 years) was conducted over eight weeks, comparing the two dietary approaches alongside an 8-week HIIT program. Body composition was assessed using bioelectrical impedance analysis (BIA) and dual-energy X-ray absorptiometry (DEXA). Performance metrics included handgrip strength and the 6-min walking test (6MWT). Salivary samples were analyzed for Orexin-A (OX-A) levels pre- and post-intervention. Results: Significant improvements in health metrics, such as heart rate (HR) and endurance, were found, with mean HR changes showing a significant difference (F = 5.943, p = 0.033) between the groups at T1. Orexin-A levels reflected significant metabolic regulation shifts in relation to other variables, showing a change from baseline to post-intervention values at T1 (F = 10,931, p = 0.033). Flexibility (sit and reach) significantly improved by 6% (p < 0.05), as well as

{VO}_{2}

max (10%, p < 0.05), both highlighted as key predictors of overall health outcomes. Additionally, Cohen’s d analyses indicated that the dietary groups exhibited notable differences in endurance, with the LCD group showing a Cohen’s d of −0.90, suggesting a large effect size compared with the control group. Conclusions: The combination of IF and HIIT is an effective nutritional strategy for enhancing body composition and physical performance in postmenopausal women, potentially mediated by changes in the orexinergic system. Further research is warranted to explore long-term effects and underlying mechanisms. Full article

(This article belongs to the Section Nutrition and Metabolism)

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13 pages, 1676 KiB

Open AccessEditor’s ChoiceArticle

The Effect of Creatine Supplementation on Lean Body Mass with and Without Resistance Training

by Imtiaz Desai, Anurag Pandit, Abbie E. Smith-Ryan, David Simar, Darren G. Candow, Nadeem O. Kaakoush and Amanda D. Hagstrom

Nutrients 2025, 17(6), 1081; https://doi.org/10.3390/nu17061081 - 19 Mar 2025

Cited by 1 | Viewed by 47160

Abstract

Background/Objectives: Creatine monohydrate (CrM) is considered to be one of the most effective supplements for enhancing lean body mass during resistance training. However, CrM may influence body water content, potentially confounding lean body mass measurements. Therefore, this randomised controlled trial assessed the [...] Read more.

Background/Objectives: Creatine monohydrate (CrM) is considered to be one of the most effective supplements for enhancing lean body mass during resistance training. However, CrM may influence body water content, potentially confounding lean body mass measurements. Therefore, this randomised controlled trial assessed the effect of CrM alone on lean body mass following a supplement wash-in, and when combined with a resistance training program. Methods: Sixty-three (34 females, 29 males, 31 ± 8 years) participants were randomised to supplement with CrM (5 g/day for 13 weeks: wash-in + 12-week resistance training) or serve as a control (received no creatine or placebo). Lean body mass was measured using dual X-ray absorptiometry at baseline, post 7-day wash-in, and post 12 weeks of resistance training. Both groups began the same training program post CrM wash-in. Results: After the 7-day wash-in, the supplement group gained 0.51 ± 1.79 kg more lean body mass than the control group (p = 0.03). Following the wash-in, both groups gained 2 kg after resistance training (p < 0.0001), with no between-group difference in lean body mass growth (p = 0.71). Sex-disaggregated analysis showed that the supplement group, only in females, gained 0.59 ± 1.61 kg more lean body mass than the controls (p = 0.04). There were no group differences in lean body mass growth following resistance training in females (p = 0.10) or males (p = 0.35). Conclusions: A 7-day CrM wash-in increased lean body mass, particularly in females. Thereafter, CrM did not enhance lean body mass growth when combined with resistance training, likely due to its short-term effects on lean body mass measurements. A maintenance dose of higher than 5 g/day may be necessary to augment lean body mass growth. Full article

(This article belongs to the Section Sports Nutrition)

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16 pages, 2059 KiB

Open AccessEditor’s ChoiceArticle

Epigenetic Aging Acceleration in Obesity Is Slowed Down by Nutritional Ketosis Following Very Low-Calorie Ketogenic Diet (VLCKD): A New Perspective to Reverse Biological Age

by Andrea G. Izquierdo, Paula M. Lorenzo, Nicolás Costa-Fraga, David Primo-Martin, Gemma Rodriguez-Carnero, Carolina F. Nicoletti, J. Alfredo Martínez, Felipe F. Casanueva, Daniel de Luis, Angel Diaz-Lagares and Ana B. Crujeiras

Nutrients 2025, 17(6), 1060; https://doi.org/10.3390/nu17061060 - 18 Mar 2025

Cited by 2 | Viewed by 6266

Abstract

Background/Objectives: Epigenetic clocks have emerged as a tool to quantify biological age, providing a more accurate estimate of an individual’s health status than chronological age, helping to identify risk factors for accelerated aging and evaluating the reversibility of therapeutic strategies. This study [...] Read more.

Background/Objectives: Epigenetic clocks have emerged as a tool to quantify biological age, providing a more accurate estimate of an individual’s health status than chronological age, helping to identify risk factors for accelerated aging and evaluating the reversibility of therapeutic strategies. This study aimed to evaluate the potential association between epigenetic acceleration of biological age and obesity, as well as to determine whether nutritional interventions for body weight loss could slow down this acceleration. Methods: Biological age was estimated using three epigenetic clocks (Horvath (Hv), Hannum (Hn), and Levine (Lv)) based on the leukocyte methylome analysis of individuals with normal weight (n = 20), obesity (n = 24), and patients with obesity following a VLCKD (n = 10). We analyzed differences in biological age estimates, the relationship between age acceleration and obesity, and the impact of VLCKD. Correlations were assessed between age acceleration, BMI, and various metabolic parameters. Results: Analysis of the epigenetic clocks revealed an acceleration of biological age in individuals with obesity (Hv = +3.4(2.5), Hn = +5.7(3.2), Lv = +3.9(2.7)) compared to a slight deceleration in individuals with normal weight. This epigenetic acceleration correlated with BMI (p < 0.0001). Interestingly, patients with obesity following a VLCKD showed a deceleration in estimated biological age, both in nutritional ketosis (Hv = −3.3(4.0), Hn = −6.3(5.3), Lv = −8.8(4.5)) and at endpoint (Hv = −1.1(4.3), Hn = −7.4(5.6), Lv = −8.2(5.3)). Relevantly, this slowdown in age is associated with BMI (p < 0.0001), ketonemia (p ≤ 0.001), and metabolic parameters (p < 0.05). Conclusions: Our findings highlight the applicability of epigenetic clocks to monitor obesity-related biological aging in precision medicine and show the potential efficacy of the VLCKD in slowing obesity-related epigenetic aging. Full article

(This article belongs to the Section Nutrigenetics and Nutrigenomics)

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15 pages, 287 KiB

Open AccessEditor’s ChoiceArticle

Early Time-Restricted Eating Improves Weight Loss While Preserving Muscle: An 8-Week Trial in Young Women

by Zifu Yu and Takeshi Ueda

Nutrients 2025, 17(6), 1022; https://doi.org/10.3390/nu17061022 - 14 Mar 2025

Cited by 1 | Viewed by 12699

Abstract

Background: Time-restricted eating (TRE) has gained attention as a novel dietary intervention that restricts the daily eating window, potentially offering improved metabolic health and body composition. Nevertheless, whether early TRE (eTRE) or delayed TRE (dTRE) best enhances resistance training (RT) adaptations remains [...] Read more.

Background: Time-restricted eating (TRE) has gained attention as a novel dietary intervention that restricts the daily eating window, potentially offering improved metabolic health and body composition. Nevertheless, whether early TRE (eTRE) or delayed TRE (dTRE) best enhances resistance training (RT) adaptations remains unclear. Methods: In this 8-week randomized study, 24 healthy young women with limited RT experience were assigned into one of three groups: eTRE (an 8:00 AM–2:00 PM feeding window), dTRE (12:00 PM–6:00 PM), or the control (8:00 AM–8:00 PM). Apart from the timing restrictions, no further dietary guidance was provided. All of the participants performed standardized knee-supported push-ups (4 sets × 10 reps, three sessions/week). The primary outcomes included body weight, the thickness of the triceps brachii long head (measured via ultrasound), and push-up endurance. Results: The eTRE group achieved a significant reduction in body weight (−2.61 ± 1.06 kg; p < 0.001), which surpassed the changes observed in both the dTRE (−1.44 ± 1.12 kg) and control (−0.48 ± 0.64 kg) groups. However, no significant between-group differences emerged for muscle thickness or push-up performance. All groups showed comparable improvements in triceps brachii thickness (a 1.36–1.55 mm increase) and push-up endurance (62–74 additional repetitions). Conclusions: Early TRE (8:00 AM–2:00 PM) appears to be more beneficial than delayed TRE (12:00 PM–6:00 PM) for weight management when combined with RT, yet both TRE regimens result in similar improvements in muscle thickness and endurance. These findings suggest that optimizing meal timing in alignment with circadian rhythms may enhance weight control without hindering muscle adaptations, providing a practical approach for individuals seeking to lose weight while preserving or increasing their muscular fitness. Future research involving larger samples and diverse populations is warranted to confirm these results and clarify the underlying metabolic mechanisms. Full article

(This article belongs to the Special Issue Effects of Nutrient Intake on Exercise Recovery and Adaptation)

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10 pages, 202 KiB

Open AccessEditor’s ChoiceReview

The Impact of Gut Microbiota on the Development of Anxiety Symptoms—A Narrative Review

by Kamil Nikel, Michał Stojko, Joanna Smolarczyk and Magdalena Piegza

Nutrients 2025, 17(6), 933; https://doi.org/10.3390/nu17060933 - 7 Mar 2025

Cited by 1 | Viewed by 7846

Abstract

The gut microbiota plays a key role in mental health, with growing evidence linking its composition to anxiety and depressive disorders. Research on this topic has expanded significantly in recent years. This review explores alterations in the gut microbiota of individuals with anxiety [...] Read more.

The gut microbiota plays a key role in mental health, with growing evidence linking its composition to anxiety and depressive disorders. Research on this topic has expanded significantly in recent years. This review explores alterations in the gut microbiota of individuals with anxiety disorders and examines the potential therapeutic effects of probiotics. Background/Objectives: This review aims to analyze the alterations in gut microbiota composition in individuals with anxiety disorders and evaluate the potential therapeutic effects of probiotics in mitigating symptoms. By examining recent research, this study seeks to highlight the gut–brain connection and its implications for mental health interventions. Materials and Methods: A literature search was conducted in PubMed, Embase, CINAHL, and Google Scholar, focusing on studies investigating the relationship between gut microbiota and anxiety disorders, as well as the impact of probiotics on symptom severity. Results: The reviewed studies suggest that individuals with anxiety disorders often exhibit gut microbiota alterations, including reduced microbial diversity and a lower abundance of short-chain fatty acid-producing bacteria. Additionally, probiotics, particularly those from the Lactobacillus genus, may help alleviate anxiety symptoms by modulating gut microbiota composition. Conclusions: Gut dysbiosis appears to be closely linked to anxiety disorders, and probiotic interventions could represent a promising therapeutic avenue. However, further research is needed to clarify underlying mechanisms and optimize treatment strategies. Full article

(This article belongs to the Special Issue The Effect of Nutrition Interventions on Neuropsychiatric Diseases)

23 pages, 2928 KiB

Open AccessEditor’s ChoiceArticle

Short-Term Magnesium Supplementation Has Modest Detrimental Effects on Cycle Ergometer Exercise Performance and Skeletal Muscle Mitochondria and Negligible Effects on the Gut Microbiota: A Randomized Crossover Clinical Trial

by Matthew C. Bomar, Taylor R. Ewell, Reagan L. Brown, David M. Brown, Beatrice S. Kwarteng, Kieran S. S. Abbotts, Hannah M. Butterklee, Natasha N. B. Williams, Scott D. Wrigley, Maureen A. Walsh, Karyn L. Hamilton, David P. Thomson, Tiffany L. Weir and Christopher Bell

Nutrients 2025, 17(5), 915; https://doi.org/10.3390/nu17050915 - 6 Mar 2025

Cited by 2 | Viewed by 6766

Abstract

Background/Objectives: Although the importance of magnesium for overall health and physiological function is well established, its influence on exercise performance is less clear. The primary study objective was to determine the influence of short-term magnesium supplementation on cycle ergometer exercise performance. The hypothesis [...] Read more.

Background/Objectives: Although the importance of magnesium for overall health and physiological function is well established, its influence on exercise performance is less clear. The primary study objective was to determine the influence of short-term magnesium supplementation on cycle ergometer exercise performance. The hypothesis was that magnesium would elicit an ergogenic effect. Methods: A randomized, double-blind, placebo-controlled, two-period crossover design was used to study men and women who were regular exercisers. Fifteen participants ingested either a placebo or magnesium chloride (MgCl₂ 300 mg) twice per day, for 9 days, separated by a 3-week washout. During days 8 and 9, participants completed a battery of cycle ergometer exercise tests, and whole blood, vastus lateralis, and stools were sampled. The primary outcomes were the maximal oxygen uptake (VO_2max), a simulated 10 km time trial, and the sprint exercise performance. Additional outcomes included skeletal muscle mitochondrial respiration, and, on account of the known laxative effects of magnesium, the gut microbiota diversity. Results: Compared with a placebo, MgCl₂ supplementation increased the circulating ionized Mg concentration (p < 0.03), decreased the VO_2max (44.4 ± 7.7 vs. 41.3 ± 8.0 mL/kg/min; p = 0.005), and decreased the mean power output during a 30 s sprint (439 ± 88 vs. 415 ± 88 W; p = 0.03). The 10 km time trial was unaffected (1282 ± 126 vs. 1281 ± 97 s; p = 0.89). In skeletal muscle, MgCl₂ decreased mitochondrial respiration in the presence of fatty acids at complex II (p = 0.04). There were no significant impacts on the gut microbiota richness (CHAO1; p = 0.68), Shannon’s Diversity (p = 0.23), or the beta-diversity (Bray–Curtis distances; p = 0.74). Conclusions: In summary, magnesium supplementation had modest ergolytic effects on cycle ergometer exercise performance and mitochondrial respiration. We recommend that regular exercisers, free from hypomagnesemia, should not supplement their diet with magnesium. Full article

(This article belongs to the Special Issue Nutritional Supplementation in Health and Sports Performance)

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20 pages, 657 KiB

Open AccessEditor’s ChoiceReview

The Detrimental Impact of Ultra-Processed Foods on the Human Gut Microbiome and Gut Barrier

by Debora Rondinella, Pauline Celine Raoul, Eleonora Valeriani, Irene Venturini, Marco Cintoni, Andrea Severino, Francesca Sofia Galli, Vincenzina Mora, Maria Cristina Mele, Giovanni Cammarota, Antonio Gasbarrini, Emanuele Rinninella and Gianluca Ianiro

Nutrients 2025, 17(5), 859; https://doi.org/10.3390/nu17050859 - 28 Feb 2025

Cited by 11 | Viewed by 15496

Abstract

Ultra-processed foods (UPFs) have become a widely consumed food category in modern diets. However, their impact on gut health is raising increasing concerns. This review investigates how UPFs impact the gut microbiome and gut barrier, emphasizing gut dysbiosis and increased gut permeability. UPFs, [...] Read more.

Ultra-processed foods (UPFs) have become a widely consumed food category in modern diets. However, their impact on gut health is raising increasing concerns. This review investigates how UPFs impact the gut microbiome and gut barrier, emphasizing gut dysbiosis and increased gut permeability. UPFs, characterized by a high content of synthetic additives and emulsifiers, and low fiber content, are associated with a decrease in microbial diversity, lower levels of beneficial bacteria like Akkermansia muciniphila and Faecalibacterium prausnitzii, and an increase in pro-inflammatory microorganisms. These alterations in the microbial community contribute to persistent inflammation, which is associated with various chronic disorders including metabolic syndrome, irritable bowel syndrome, type 2 diabetes, and colorectal cancer. In addition, UPFs may alter the gut–brain axis, potentially affecting cognitive function and mental health. Dietary modifications incorporating fiber, fermented foods, and probiotics can help mitigate the effects of UPFs. Furthermore, the public needs stricter regulations for banning UPFs, along with well-defined food labels. Further studies are necessary to elucidate the mechanisms connecting UPFs to gut dysbiosis and systemic illnesses, thereby informing evidence-based dietary guidelines. Full article

(This article belongs to the Special Issue Nutrition, Gut Microbiota and Immunity)

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35 pages, 1868 KiB

Open AccessEditor’s ChoiceReview

Impact of Vegan and Vegetarian Diets on Neurological Health: A Critical Review

by Vicente Javier Clemente-Suárez, Laura Redondo-Flórez, Alexandra Martín-Rodríguez, Agustín Curiel-Regueros, Alejandro Rubio-Zarapuz and José Francisco Tornero-Aguilera

Nutrients 2025, 17(5), 884; https://doi.org/10.3390/nu17050884 - 28 Feb 2025

Cited by 6 | Viewed by 18570

Abstract

Background/Objectives: The global shift towards vegan and vegetarian diets has garnered attention for their ethical, environmental, and potential health benefits. These diets are often rich in phytonutrients and antioxidants, which have been associated with lower levels of inflammatory markers, such as C-reactive protein [...] Read more.

Background/Objectives: The global shift towards vegan and vegetarian diets has garnered attention for their ethical, environmental, and potential health benefits. These diets are often rich in phytonutrients and antioxidants, which have been associated with lower levels of inflammatory markers, such as C-reactive protein (CRP) and interleukin-6 (IL-6), suggesting a potential protective effect against systemic inflammation and oxidative stress. However, despite these benefits, concerns remain regarding their impact on neurological health due to the possible deficiencies of critical nutrients such as vitamin B12, DHA, EPA, and iron. This review critically evaluates the influence of these dietary patterns on neurological outcomes, emphasizing their nutritional composition, potential deficiencies, and their interplay with inflammation and oxidative stress. Methods: A systematic review of the literature published between 2010 and 2023 was conducted, focusing on studies that explore the relationship between vegan and vegetarian diets and neurological health. Key nutrients such as vitamin B12, omega-3 fatty acids, iron, and zinc were analyzed alongside antinutritional factors and their effects on the nervous system. Results: Evidence suggests that vegan and vegetarian diets, when well planned, can be rich in phytonutrients and antioxidants, which have been associated with lower levels of inflammatory markers, such as C-reactive protein (CRP) and interleukin-6 (IL-6). These findings indicate a potential role in reducing systemic inflammation and oxidative stress, both of which are linked to neurodegenerative diseases. However, deficiencies in critical nutrients such as vitamin B12, DHA, EPA, and iron have been consistently associated with an increased risk of cognitive decline, mood disturbances, and neurodegenerative disorders. Additionally, the presence of antinutritional factors like phytates and oxalates may further impair nutrient absorption, necessitating careful dietary planning and supplementation. Conclusions: While plant-based diets provide anti-inflammatory and antioxidant benefits, their neurological implications depend on nutrient adequacy. Proper planning, supplementation, and food preparation techniques are essential to mitigate risks and enhance cognitive health. Further research is needed to explore long-term neurological outcomes and optimize dietary strategies. Full article

(This article belongs to the Special Issue Neuro-Nutrition in Sports: Advances in Functional Neurology and Metabolic Care)

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16 pages, 1157 KiB

Open AccessEditor’s ChoiceArticle

The Effects of Combined Scutellaria and Saffron Supplementation on Mood Regulation in Participants with Mild-to-Moderate Depressive Symptoms: A Randomized, Double-Blind, Placebo-Controlled Study

by Valérie Dormal, Marlène Suchareau, Sylvie Copine, Laurent Simar and Louise Deldicque

Nutrients 2025, 17(5), 809; https://doi.org/10.3390/nu17050809 - 26 Feb 2025

Cited by 3 | Viewed by 9696

Abstract

Background/Objectives: The global prevalence of mental health disorders, particularly anxiety and depression, has increased significantly, with rates further elevated by the COVID-19 pandemic. Conventional pharmacological treatments, while effective, often lead to side effects that can impact patient adherence and quality of life. This [...] Read more.

Background/Objectives: The global prevalence of mental health disorders, particularly anxiety and depression, has increased significantly, with rates further elevated by the COVID-19 pandemic. Conventional pharmacological treatments, while effective, often lead to side effects that can impact patient adherence and quality of life. This has driven interest in safer, natural adjunctive therapies. Crocus sativus L. (Iridaceae) (saffron) and Scutellaria baicalensis Georgi (Lamiaceae) (scutellaria) have individually shown potential, in humans and animals, respectively, as mood regulators, with bioactive compounds that modulate neurotransmitter systems and possess anti-inflammatory and anxiolytic effects. This study aimed (1) to explore the efficacy and safety of scutellaria extracts in humans and (2) to test a possible synergistic effect when combining scutellaria and saffron on mood regulation in individuals experiencing mild-to-moderate depressive symptoms. Methods: In a randomized, double-blind, placebo-controlled trial, 180 participants with mild-to-moderate depressive symptoms were assigned to receive either scutellaria extract alone (SCUTELL’UP^®), saffron extract alone (SAFFR’ACTIV^®), a combination of scutellaria and saffron extracts (SAFFR’UP^®), or a placebo for six weeks. The primary outcome was assessed using a standardized depression scale (Beck Depression Inventory). The secondary outcomes, including anxiety, emotional state, well-being level, and sleep quality, were all assessed using validated questionnaires. Safety and tolerability were evaluated throughout the study period. Results: The results confirmed the beneficial effects of saffron extract on depressive and anxious symptoms, as well as its role in improving sleep quality. For the first time in humans, scutellaria extract demonstrated a positive effect on mood regulation. Furthermore, a synergistic effect of the combination of these two extracts was identified, leading to enhanced improvements in depressive and anxious symptoms and emotional well-being among individuals with mild-to-moderate depression, compared to the placebo group. Minimal adverse effects were reported across all treatment groups. Conclusions: This natural adjunctive nutritional strategy offers a promising alternative for individuals seeking safer options for mental health support. Further research is warranted to exclude potential long-term side effects and to explore potential mechanisms of this combined supplementation. Full article

(This article belongs to the Section Nutrition and Neuro Sciences)

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20 pages, 1888 KiB

Open AccessEditor’s ChoiceArticle

The Cholesterol Paradox in Long-Livers from a Sardinia Longevity Hot Spot (Blue Zone)

by Alessandra Errigo, Maria Pina Dore, Michele Portoghese and Giovanni Mario Pes

Nutrients 2025, 17(5), 765; https://doi.org/10.3390/nu17050765 - 21 Feb 2025

Viewed by 16870

Abstract

Background/Objectives: Hypercholesterolemia is commonly viewed as a risk factor for coronary heart disease; however, several studies have reported an inverse relationship between cholesterol levels and cardiovascular mortality, particularly in older adults. This “cholesterol paradox” challenges the conventional understanding of lipid metabolism. Despite [...] Read more.

Background/Objectives: Hypercholesterolemia is commonly viewed as a risk factor for coronary heart disease; however, several studies have reported an inverse relationship between cholesterol levels and cardiovascular mortality, particularly in older adults. This “cholesterol paradox” challenges the conventional understanding of lipid metabolism. Despite often being dismissed as a result of reverse causality, the precise causes of this paradox remain poorly understood. This study aimed to investigate the potential existence of the cholesterol paradox in a long-lived population from central Sardinia, Italy. Methods: We recruited 168 baseline nonagenarians (81 males, 87 females) from the longevity Blue Zone area in 2018 and followed them until December 2024. The lipid profile was determined for all participants according to current guidelines, and its impact on survival was analyzed with Kaplan–Meier curves and Cox proportional hazards regression models. Results: The median total cholesterol was 199.5 (range 89–314) mg/dL in males and 202.5 (range 89–324) mg/dL in females. Survival time was significantly longer in participants with LDL cholesterol (LDL-C) above 130 mg/dL compared to that in nonagenarians with LDL-C lower than 130 mg/dL (3.82 ± 1.88 years vs. 2.79 ± 1.56 years, p < 0.0001). Cox regression analysis revealed a significant reduction in the hazard ratio (HR) for mortality in participants with mild hypercholesterolemia (LDL-C ≥ 130 mg/dL) compared to that in those with normal cholesterol (OR 0.600, 95%CI 0.405–0.891). Conclusions: In the long-lived population examined, the cholesterol paradox was unlikely to be a reflection of reverse causality. Our results challenge the common view that longevity is invariably associated with low cholesterol levels. Furthermore, moderate hypercholesterolemia does not preclude the oldest adult from attaining advanced ages, contrary to common belief. Full article

(This article belongs to the Section Geriatric Nutrition)

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19 pages, 664 KiB

Open AccessEditor’s ChoiceArticle

In Vitro Evaluation of Bioavailability of Mg from Daily Food Rations, Dietary Supplements and Medicinal Products from the Polish Market

by Piotr Bawiec, Agnieszka Jaworowska, Jan Sawicki, Marcin Czop, Radosław Szalak and Wojciech Koch

Nutrients 2025, 17(5), 748; https://doi.org/10.3390/nu17050748 - 20 Feb 2025

Viewed by 7078

Abstract

Background: Nutrients consumed with food undergo the digestion process, and only some of them are absorbed from the gastrointestinal tract (GI) and enter the bloodstream. Minerals, including Mg, are crucial for maintaining the body’s homeostasis, but their beneficial effect depends on their bioavailability, [...] Read more.

Background: Nutrients consumed with food undergo the digestion process, and only some of them are absorbed from the gastrointestinal tract (GI) and enter the bloodstream. Minerals, including Mg, are crucial for maintaining the body’s homeostasis, but their beneficial effect depends on their bioavailability, i.e., the part that can be absorbed and used by the body. The bioavailability of nutrients taken in pharmaceutical form is usually higher than the same nutrients contained in food, because their absorption requires prior release from the food matrix. Objectives: The main objective of the conducted research was to assess the bioavailability of Mg from dietary supplements and medicinal products, considering the influence of the type of diet and pharmaceutical form on bioavailability. Methods: The experiments were conducted using a previously developed and optimized two-stage in vitro digestion model using cellulose dialysis tubes and ICP-OES method. The influence of three types of diets—standard, basic and high-residue—on the bioavailability of Mg was evaluated. Results: The bioavailability of Mg from the studied diets was within the range of 48.74–52.51%. Conclusions: In the models studied, it was observed that the factors influencing bioavailability were the nutritional composition of the diets and the chemical form of Mg. Full article

(This article belongs to the Section Phytochemicals and Human Health)

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26 pages, 2854 KiB

Open AccessEditor’s ChoiceReview

Magnesium and Migraine

by Ligia J. Dominguez, Nicola Veronese, Shaun Sabico, Nasser M. Al-Daghri and Mario Barbagallo

Nutrients 2025, 17(4), 725; https://doi.org/10.3390/nu17040725 - 18 Feb 2025

Cited by 2 | Viewed by 14410

Abstract

Migraine is a widespread and intricate neurological condition that involves various factors and is marked by recurring headache episodes. Migraine is among the ten neurological conditions accounting for the greatest disability in the whole population, the leading cause of disability for children and [...] Read more.

Migraine is a widespread and intricate neurological condition that involves various factors and is marked by recurring headache episodes. Migraine is among the ten neurological conditions accounting for the greatest disability in the whole population, the leading cause of disability for children and adolescents aged 5–19 years, and the second cause of disability for adults aged 20–59 years. Magnesium deficiency is also a very common condition resulting from diverse reasons, including insufficient dietary consumption or increased loss through the gastrointestinal or renal system. Accumulated evidence from case reports, case–control studies, observational studies, and randomized, placebo-controlled trials has shown the effectiveness of magnesium supplementation in alleviating migraine, both acutely and chronically. Mechanisms that may help explain these results include the potential link between magnesium deficit and spreading cortical depression, vascular changes, oxidative stress, chronic inflammation, nervous excitation, neurotransmitter release, and electrolyte imbalances. This article aims to provide a comprehensive review of the available evidence on the links between magnesium and migraine, considering the role of magnesium in the pathogenesis of migraine and the utility of magnesium in its prevention and treatment. Full article

(This article belongs to the Section Micronutrients and Human Health)

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21 pages, 1410 KiB

Open AccessEditor’s ChoiceReview

The Influence of Physical Exercise, Ketogenic Diet, and Time-Restricted Eating on De Novo Lipogenesis: A Narrative Review

by Antonio Paoli

Nutrients 2025, 17(4), 663; https://doi.org/10.3390/nu17040663 - 13 Feb 2025

Cited by 3 | Viewed by 7237

Abstract

De novo lipogenesis (DNL) is a metabolic pathway that converts carbohydrates into fatty acids, primarily occurring in the liver and, to a lesser extent, in adipose tissue. While hepatic DNL is highly responsive to dietary carbohydrate intake and regulated by insulin via transcription [...] Read more.

De novo lipogenesis (DNL) is a metabolic pathway that converts carbohydrates into fatty acids, primarily occurring in the liver and, to a lesser extent, in adipose tissue. While hepatic DNL is highly responsive to dietary carbohydrate intake and regulated by insulin via transcription factors like SREBP-1c, adipose DNL is more modest and less sensitive to dietary overfeeding. Dysregulated DNL contributes to metabolic disorders, including metabolic dysfunction-associated steatotic liver disease (MASLD). Lifestyle interventions, such as physical exercise, ketogenic diets, and time-restricted eating (TRE) offer promising strategies to regulate DNL and improve metabolic health. Physical exercise enhances glucose uptake in muscles, reduces insulin levels, and promotes lipid oxidation, thereby suppressing hepatic DNL. Endurance and resistance training also improve mitochondrial function, further mitigating hepatic triglyceride accumulation. Ketogenic diets shift energy metabolism toward fatty acid oxidation and ketogenesis, lower insulin, and directly downregulate lipogenic enzyme activity in the liver. TRE aligns feeding with circadian rhythms by optimizing AMP-activated protein kinase (AMPK) activation during fasting periods, which suppresses DNL and enhances lipid metabolism. The combined effects of these interventions demonstrate significant potential for improving lipid profiles, reducing hepatic triglycerides, and preventing lipotoxicity. By addressing the distinct roles of the liver and adipose DNL, these strategies target systemic and localized lipid metabolism dysregulation. Although further research is needed to fully understand their long-term impact, these findings highlight the transformative potential of integrating these approaches into clinical practice to manage metabolic disorders and their associated complications. Full article

(This article belongs to the Special Issue Effect of Dietary, Nutrition and Physical Activity on Body Composition)

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42 pages, 684 KiB

Open AccessEditor’s ChoiceReview

The Influence of Dietary Patterns on Polycystic Ovary Syndrome Management in Women: A Review of Randomized Controlled Trials with and Without an Isocaloric Dietary Design

by Yeonjeong Choi, Kyeonghoon Kang, Minkyung Je, Young-Min Lee and Yoona Kim

Nutrients 2025, 17(4), 674; https://doi.org/10.3390/nu17040674 - 13 Feb 2025

Viewed by 6229

Abstract

Polycystic ovary syndrome (PCOS) is an endocrine disorder that causes cardiometabolic and reproductive disorders in women of reproductive age. Women with PCOS are more likely to have obesity, type 2 diabetes mellitus, and cardiovascular disease. There is an inconclusive consensus on which dietary [...] Read more.

Polycystic ovary syndrome (PCOS) is an endocrine disorder that causes cardiometabolic and reproductive disorders in women of reproductive age. Women with PCOS are more likely to have obesity, type 2 diabetes mellitus, and cardiovascular disease. There is an inconclusive consensus on which dietary modification could be most effective in PCOS prevention and treatment. This review aimed to examine the effects of diverse dietary patterns on PCOS in women according to randomized controlled trials (RCTs) with and without an isocaloric dietary design. A literature search was performed in the PubMed^®®/MEDLINE^®® database up to 14 November 2024. A total of 21 RCTs were reviewed after screening the records, including 15 RCTs with a calorie-restricted dietary design and 6 RCTs with a non-calorie-restricted dietary design. This review found beneficial effects of the calorie-restricted Dietary Approaches to Stop Hypertension (DASH) diet on weight loss and glucose control in women with PCOS in four RCTs with an isocaloric dietary design. The calorie-restricted low-glycemic index (GI) diets from three RCTs and high-protein diets from four RCTs with an isocaloric dietary design showed no significant differences in anthropometric parameters, glucose control, lipids, and gonadal parameters compared with the control diet in women with PCOS. Non-calorie-restricted low-carbohydrate diets from four RCTs with an isocaloric dietary design showed similar results to the calorie-restricted low-GI diets and high-protein diets. However, the existing number of RCTs is insufficient to conclude the association between dietary patterns and PCOS in women. Further, well-designed dietary intervention studies are needed to assess the role of dietary patterns in PCOS beyond calorie restriction. Full article

(This article belongs to the Section Nutrition in Women)

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12 pages, 731 KiB

Open AccessEditor’s ChoiceArticle

Impact of Polyphenol-Rich Nutraceuticals on Cognitive Function and Neuroprotective Biomarkers: A Randomized, Double-Blind, Placebo-Controlled Clinical Trial

by Juan Ángel Carrillo, Raúl Arcusa, Raquel Xandri-Martínez, Begoña Cerdá, Pilar Zafrilla and Javier Marhuenda

Nutrients 2025, 17(4), 601; https://doi.org/10.3390/nu17040601 - 7 Feb 2025

Cited by 5 | Viewed by 5278

Abstract

Background: Recent studies have highlighted the neuroprotective effects of polyphenols, particularly their role in enhancing brain-derived neurotrophic factor (BDNF) and cAMP response element-binding protein (CREB) activity. This study aimed to evaluate the relationship between BDNF and CREB levels and cognitive performance in individuals [...] Read more.

Background: Recent studies have highlighted the neuroprotective effects of polyphenols, particularly their role in enhancing brain-derived neurotrophic factor (BDNF) and cAMP response element-binding protein (CREB) activity. This study aimed to evaluate the relationship between BDNF and CREB levels and cognitive performance in individuals undergoing a polyphenol-rich dietary intervention. Methods: A randomized, crossover, double-blind, placebo-controlled clinical trial was conducted with 92 participants. The intervention involved the daily intake of an encapsulated concentrate of fruit, vegetable, and berry juice powders (Juice Plus+ Premium^®) over two 16-week periods, separated by a 4-week washout phase. Cognitive function was assessed using the Stroop Test, Trail Making Test, and Reynolds Intellectual Screening Test (RIST). The plasma levels of CREB and BDNF were measured using ELISA. Results: The polyphenol-rich product significantly improved cognitive performance, as evidenced by higher scores in the Stroop Test and RIST, compared to the placebo. Additionally, the plasma levels of CREB and BDNF were notably elevated in the product condition, indicating enhanced neuroprotective activity. Conclusions: The findings suggest that polyphenol-rich nutraceuticals can modulate neurobiological mechanisms underlying cognitive improvements, primarily through the reduction of oxidative stress and the regulation of signaling pathways associated with synaptic plasticity. These results support the potential of dietary polyphenols in promoting cognitive health and preventing neurodegenerative diseases. Full article

(This article belongs to the Special Issue Sensory Nutrition and Health Impact on Metabolic and Brain Disorders)

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40 pages, 673 KiB

Open AccessEditor’s ChoiceSystematic Review

Mediterranean Diet and Quality of Life in Adults: A Systematic Review

by Justyna Godos, Monica Guglielmetti, Cinzia Ferraris, Evelyn Frias-Toral, Irma Domínguez Azpíroz, Vivian Lipari, Andrea Di Mauro, Fabrizio Furnari, Sabrina Castellano, Fabio Galvano, Licia Iacoviello, Marialaura Bonaccio and Giuseppe Grosso

Nutrients 2025, 17(3), 577; https://doi.org/10.3390/nu17030577 - 5 Feb 2025

Cited by 19 | Viewed by 11543

Abstract

Background/Objectives: With the increasing life expectancy and, as a result, the aging of the global population, there has been a rise in the prevalence of chronic conditions, which can significantly impact individuals’ health-related quality of life, a multidimensional concept that comprises an individual’s [...] Read more.

Background/Objectives: With the increasing life expectancy and, as a result, the aging of the global population, there has been a rise in the prevalence of chronic conditions, which can significantly impact individuals’ health-related quality of life, a multidimensional concept that comprises an individual’s physical, mental, and social wellbeing. While a balanced, nutrient-dense diet, such as Mediterranean diet, is widely recognized for its role in chronic disease prevention, particularly in reducing the risk of cardiovascular diseases and certain cancers, its potential benefits extend beyond these well-known effects, showing promise in improving physical and mental wellbeing, and promoting health-related quality of life. Methods: A systematic search of the scientific literature in electronic databases (Pubmed/Medline) was performed to identify potentially eligible studies reporting on the relation between adherence to the Mediterranean diet and health-related quality of life, published up to December 2024. Results: A total of 28 studies were included in this systematic review, comprising 13 studies conducted among the general population and 15 studies involving various types of patients. Overall, most studies showed a significant association between adherence to the Mediterranean diet and HRQoL, with the most significant results retrieved for physical domains of quality of life, suggesting that diet seems to play a relevant role in both the general population and people affected by chronic conditions with an inflammatory basis. Conclusions: Adherence to the Mediterranean diet provides significant benefits in preventing and managing various chronic diseases commonly associated with aging populations. Furthermore, it enhances the overall health and quality of life of aging individuals, ultimately supporting more effective and less invasive treatment approaches for chronic diseases. Full article

(This article belongs to the Special Issue Mediterranean Diet and Chronic Diseases)

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17 pages, 948 KiB

Open AccessEditor’s ChoiceReview

Consumption of Red Versus White Wine and Cancer Risk: A Meta-Analysis of Observational Studies

by Rachel K. Lim, Jongeun Rhee, Megan Hoang, Abrar A. Qureshi and Eunyoung Cho

Nutrients 2025, 17(3), 534; https://doi.org/10.3390/nu17030534 - 31 Jan 2025

Cited by 4 | Viewed by 10195

Abstract

Background/Objectives: While alcoholic beverage consumption increases cancer risk, red wine has been touted as a healthier option. To address this unexplored question, we conducted a meta-analysis to summarize evidence from observational studies. Methods: A literature search of PubMed and EMBASE through December 2023 [...] Read more.

Background/Objectives: While alcoholic beverage consumption increases cancer risk, red wine has been touted as a healthier option. To address this unexplored question, we conducted a meta-analysis to summarize evidence from observational studies. Methods: A literature search of PubMed and EMBASE through December 2023 identified studies examining wine and cancer risk. A random-effects meta-analysis was performed to estimate relative risks (RRs) and 95% confidence intervals (CIs) for an association between wine intake and overall cancer risk. Results: A total of 20 cohort and 22 case–control studies were included. Wine intake was not associated with overall cancer risk (n = 95,923) when comparing the highest vs. lowest levels of consumption, with no differences observed by wine type (red: summary RR = 0.98 [95% CI = 0.87, 1.10], white: 1.00 [0.91, 1.10]; P_difference = 0.74). However, white wine intake was significantly associated with an increased risk of cancer among women (white: 1.26 [1.05, 1.52], red: 0.91 [95% CI: 0.72, 1.16], P_difference = 0.03) and in analyses restricted to cohort studies (white: 1.12 [1.03, 1.22], red: 1.02 [95% CI: 0.96, 1.09], P_difference = 0.02). For individual cancer sites, there was a significant difference in associations between red and white wine intake only in skin cancer risk [6 studies, white: 1.22 (1.14, 1.30), red: 1.02 (0.95, 1.09); P_difference = 0.0003]. Conclusions: We found no differences in the association between red or white wine consumption and overall cancer risk, challenging the common belief that red wine is healthier than white wine. Our significant results related to white wine intake in subgroup analyses warrant further investigation. Full article

(This article belongs to the Special Issue Nutritional Epidemiology of Non-communicable Diseases—Feature Papers)

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10 pages, 764 KiB

Open AccessEditor’s ChoiceArticle

Egg Consumption: Trends Over 48 Years, Patterns Across the Lifespan, and Predictors of Intake

by Donna Kritz-Silverstein and Ricki Bettencourt

Nutrients 2025, 17(3), 533; https://doi.org/10.3390/nu17030533 - 31 Jan 2025

Cited by 1 | Viewed by 5907

Abstract

Background/Objectives: Eggs are an excellent nutritional source. However, historical associations of dietary cholesterol with serum cholesterol and cardiovascular disease, along with restrictive dietary guidelines may have been barriers to egg consumption. This study examines trends over time, patterns, and predictors of egg consumption [...] Read more.

Background/Objectives: Eggs are an excellent nutritional source. However, historical associations of dietary cholesterol with serum cholesterol and cardiovascular disease, along with restrictive dietary guidelines may have been barriers to egg consumption. This study examines trends over time, patterns, and predictors of egg consumption in individuals followed for 48 years, and current barriers to usage. Methods: Participants were 6326 men and women enrolled in the Rancho Bernardo Study in 1972–1974 when asked about the number of eggs consumed/week. Subsequent egg intake was collected with food frequency questionnaires during clinic visits in 1988–1991 (n = 1627) and 1992–1996 (n = 1385), and with the original question on a 2021 mailed survey (n = 710), when barriers to intake were also queried. Results: The mean (±SD) number of eggs consumed was 3.6 ± 3.0 in 1972–1974, 1.8 ± 2.1 in 1988–1991, 1.8 ± 2.2 in 1992–1996, and 3.4 ± 3.5 in 2021. Comparisons within 5-year categories of enrollment age (<20, 20–24, 25–29, 30–34, 35–39, >40) showed no differences in egg intake between 1972 and 1974, and when older in 2021. Men consumed more eggs than women at all timepoints (p’s < 0.0001). High cholesterol and taking cholesterol-lowering medication were associated with lower egg consumption in 1972–1974 (p’s < 0.0001), but were not associated in 2021. Over 22% reported limiting egg intake in 2021; the majority cited cholesterol content of eggs or having high cholesterol as barriers. Conclusions: Egg consumption was responsive to dietary guidelines, decreasing over time then increasing by 2021 to levels similar to those of the early 1970s. Despite the abandonment of these guidelines, they continue to have a negative impact for a segment of society, suggesting the need for additional education on the health benefits of eggs. Full article

(This article belongs to the Section Nutritional Epidemiology)

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27 pages, 4544 KiB

Open AccessEditor’s ChoiceSystematic Review

Dietary Patterns, Oxidative Stress, and Early Inflammation: A Systematic Review and Meta-Analysis Comparing Mediterranean, Vegan, and Vegetarian Diets

by Sara Ilari, Stefania Proietti, Francesca Milani, Laura Vitiello, Carolina Muscoli, Patrizia Russo and Stefano Bonassi

Nutrients 2025, 17(3), 548; https://doi.org/10.3390/nu17030548 - 31 Jan 2025

Cited by 7 | Viewed by 6081

Abstract

Background: Dietary habits influenced by lifestyle and cultural factors play a critical role in health by modulating oxidative stress and inflammation. While diets offer significant benefits, they may also pose risks, such as nutrient deficiencies, emphasizing the need for a balanced approach. Exploring [...] Read more.

Background: Dietary habits influenced by lifestyle and cultural factors play a critical role in health by modulating oxidative stress and inflammation. While diets offer significant benefits, they may also pose risks, such as nutrient deficiencies, emphasizing the need for a balanced approach. Exploring Mediterranean and plant-based diet effects on oxidative stress and inflammation biomarkers may help improve health outcomes and disease prevention strategies. Methods: This study analyzed 65 studies following PRISMA guidelines to evaluate the effects of Mediterranean and plant-based diets on biomarkers of oxidative stress and inflammation in healthy individuals. Results: The Mediterranean diet was weakly associated with reductions in oxidative stress markers, including MDA (ROM: 0.80; 95% CI: 0.57–1.13; p = 0.2092) and 8OHdG (ROM: 0.81; 95% CI: 0.59–1.11; p = 0.1847), as well as inflammation markers such as CRP (ROM: 0.72; 95% CI: 0.42–1.23; p = 0.1545) and IL-6 (ROM: 1.23; 95% CI: 0.97–1.55; p = 0.08). The vegetarian diet significantly reduced CRP (ROM: 0.82; 95% CI: 0.69–0.98; p = 0.0297), while the vegan diet showed a borderline reduction (ROM: 0.81; 95% CI: 0.56–1.17; p = 0.2544), suggesting lower systemic inflammation compared to omnivorous diets. Conclusions: Although all three diets demonstrate potential in reducing oxidative stress and inflammation, the antioxidant effects—especially for the Mediterranean diet—are lower than anticipated, indicating alternative mechanisms. Further research is essential to confirm these findings and clarify the underlying mechanisms to enhance preventive health strategies. Full article

(This article belongs to the Section Nutritional Epidemiology)

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17 pages, 13800 KiB

Open AccessEditor’s ChoiceReview

Low-FODMAP Diet for Irritable Bowel Syndrome: Insights from Microbiome

by Haoshuai Zhang and Qi Su

Nutrients 2025, 17(3), 544; https://doi.org/10.3390/nu17030544 - 31 Jan 2025

Cited by 2 | Viewed by 8609

Abstract

Irritable bowel syndrome (IBS) is a prevalent gastrointestinal disorder characterized by chronic abdominal pain, bloating, and altered bowel habits. Low-FODMAP diets, which involve restricting fermentable oligosaccharides, disaccharides, monosaccharides, and polyols, have emerged as an effective dietary intervention for alleviating IBS symptoms. This review [...] Read more.

Irritable bowel syndrome (IBS) is a prevalent gastrointestinal disorder characterized by chronic abdominal pain, bloating, and altered bowel habits. Low-FODMAP diets, which involve restricting fermentable oligosaccharides, disaccharides, monosaccharides, and polyols, have emerged as an effective dietary intervention for alleviating IBS symptoms. This review paper aims to synthesize current insights into the impact of a low-FODMAP diet on the gut microbiome and its mechanisms of action in managing IBS. We explore the alterations in microbial composition and function associated with a low-FODMAP diet and discuss the implications of these changes for gut health and symptom relief. Additionally, we examine the balance between symptom improvement and potential negative effects on microbial diversity and long-term gut health. Emerging evidence suggests that while a low-FODMAP diet can significantly reduce IBS symptoms, it may also lead to reductions in beneficial microbial populations. Strategies to mitigate these effects, such as the reintroduction phase and the use of probiotics, are evaluated. This review highlights the importance of a personalized approach to dietary management in IBS, considering individual variations in microbiome responses. Understanding the intricate relationship between diet, the gut microbiome, and IBS symptomatology will guide the development of more effective, sustainable dietary strategies for IBS patients. Full article

(This article belongs to the Special Issue Nutritional Therapy of Intestinal Diseases and Intestinal Microecology)

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17 pages, 1840 KiB

Open AccessEditor’s ChoiceArticle

Mango Consumption Is Associated with Increased Insulin Sensitivity in Participants with Overweight/Obesity and Chronic Low-Grade Inflammation

by Katherine D Pett, Peter Geevarghese Alex, Casey Weisfuss, Amandeep Sandhu, Britt Burton-Freeman and Indika Edirisinghe

Nutrients 2025, 17(3), 490; https://doi.org/10.3390/nu17030490 - 29 Jan 2025

Cited by 1 | Viewed by 11756

Abstract

Background/Objectives: Chronic low-grade inflammation is associated with insulin resistance and poor glycemic control, leading to the development of type 2 diabetes mellitus (T2DM). The present study investigated the effect of regular mango intake on inflammation and insulin sensitivity in participants with overweight or [...] Read more.

Background/Objectives: Chronic low-grade inflammation is associated with insulin resistance and poor glycemic control, leading to the development of type 2 diabetes mellitus (T2DM). The present study investigated the effect of regular mango intake on inflammation and insulin sensitivity in participants with overweight or obesity and chronic low-grade inflammation. Methods: A human clinical study was performed using a randomized, controlled, two-arm, parallel design with a 2 h oral glucose tolerance test (OGTT) administered before and after 4 weeks (4 W) of mango or control product intake (1 cup/twice a day). Fasting and time course blood sampling for 2 h post-OGTT were analyzed for effects on plasma metabolic and inflammation endpoints using analysis of covariance and repeated-measure approaches (SAS 9.4). Results: Forty-eight adults (37.6 ± 2.8 years, 30.5 ± 4.1 BMI kg/m²) completed the study. Markers of inflammation (IL-6, TNFα, hs-CRP) were not different at the end of 4 W (p > 0.05). The intervention did not significantly influence fasting glucose concentrations; however, insulin was significantly lowered with the mango compared to the control intervention (8.2 ± 1.2 vs. 15.3 ± 1.2 µIU/mL respectively, p = 0.05). Furthermore, the Homeostasis Model Assessment of Insulin Resistance (HOMA-IR), along with the disposition index (DI), was significantly improved in the mango compared to the control interventions (HOMA-IR, 2.28 ± 1.19 vs. 4.67 ± 1.21, p = 0.03; DI, 2.76 ± 1.02 vs. 5.37 ± 1.03, p = 0.04). Mean insulin concentrations were also significantly lower at W4 compared to W0 after the OGTT in the mango vs. control intervention (intervention × week effect, p = 0.04). Relative expression of nuclear factor erythroid 2-related factor 2 (Nrf-2), a gene regulating endogenous antioxidant defense, was non-significantly increased twofold in the mango intervention (W4 vs. W0). Conclusions: Collectively, the data suggest that mango intake increased insulin sensitivity in individuals with chronic low-grade inflammation, possibly through activating Nrf-2 genes and increasing cellular antioxidant status. The data warrant further research on consuming mango fruit as part of a dietary pattern to address insulin resistance and the mechanisms underpinning the actions of mango intake. Full article

(This article belongs to the Special Issue Diet and Disease Development: Mechanisms, Prevention and Treatment—2nd Edition)

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28 pages, 928 KiB

Open AccessEditor’s ChoiceSystematic Review

Dietary Supplementation for Fatigue Symptoms in Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS)—A Systematic Review

by Marie Celine Dorczok, Gloria Mittmann, Nilufar Mossaheb, Beate Schrank, Lucie Bartova, Matthias Neumann and Verena Steiner-Hofbauer

Nutrients 2025, 17(3), 475; https://doi.org/10.3390/nu17030475 - 28 Jan 2025

Cited by 1 | Viewed by 9148

Abstract

Background/Objectives: Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS) is a complex neuroimmunological disorder with limited treatment options. Despite the widespread use of Dietary Supplements (DSs) among ME/CFS patients to alleviate fatigue and associated symptoms, evidence remains inconclusive. This systematic review aims to provide an [...] Read more.

Background/Objectives: Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS) is a complex neuroimmunological disorder with limited treatment options. Despite the widespread use of Dietary Supplements (DSs) among ME/CFS patients to alleviate fatigue and associated symptoms, evidence remains inconclusive. This systematic review aims to provide an updated synthesis of the efficacy of DS interventions and explore possible mechanisms underlying their therapeutic effects. Methods: This systematic review was conducted according to PRISMA guidelines. Several databases (Ebsco Host, PubMed, Scopus, Google Scholar) were used for the systematic search, which was based on the broad search terms ME/CFS and DS with a focus on publications between 1994 and 2024. The primary outcome was fatigue, with additional considerations including psychological well-being, physical activity, and biochemical markers. Two independent researchers screened the studies for eligibility in a multi-stage process and assessed quality and bias using Cochrane’s risk of bias tools (RoB-2, ROBINS-I). Results: Fourteen studies (N = 809) of heterogeneous designs were included, showing a high risk of bias, mostly due to missing data and selection bias. While some interventions (L-carnitine and guanidinoacetic acid, oxaloacetate, CoQ10–selenium combination, NADH and NADH-CoQ10 combination) showed significant reductions in fatigue, methodological limitations, like small sample sizes and missing data, prevent firm conclusions. Mixed results were reported for secondary outcomes like cognitive function and inflammatory markers. Six studies noted adverse effects, including nausea and insomnia. Conclusions: Though some DSs showed potential in reducing fatigue in ME/CFS, methodological limitations and inconsistent results hinder definitive conclusions. Future research should improve diagnostic criteria and include more diverse populations. Full article

(This article belongs to the Section Clinical Nutrition)

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21 pages, 3591 KiB

Open AccessEditor’s ChoiceArticle

Effects of Vegetable and Fruit Juicing on Gut and Oral Microbiome Composition

by Maria Luisa Savo Sardaro, Veronika Grote, Jennifer Baik, Marco Atallah, Katherine Ryan Amato and Melinda Ring

Nutrients 2025, 17(3), 458; https://doi.org/10.3390/nu17030458 - 27 Jan 2025

Cited by 1 | Viewed by 24005

Abstract

Background: In recent years, juicing has often been promoted as a convenient way to increase fruit and vegetable intake, with juice-only diets marketed for digestive cleansing and overall health improvement. However, juicing removes most insoluble fiber, which may diminish the health benefits of [...] Read more.

Background: In recent years, juicing has often been promoted as a convenient way to increase fruit and vegetable intake, with juice-only diets marketed for digestive cleansing and overall health improvement. However, juicing removes most insoluble fiber, which may diminish the health benefits of whole fruits and vegetables. Lower fiber intake can alter the microbiota, affecting metabolism, immunity, and mental health, though little is known about juicing’s specific effects on the microbiota. This study addresses this gap by exploring how juicing impacts gut and oral microbiome composition in an intervention study. Methods: Fourteen participants followed one of three diets—exclusive juice, juice plus food, or plant-based food—for three days. Microbiota samples (stool, saliva, and inner cheek swabs) were collected at baseline, after a pre-intervention elimination diet, immediately after juice intervention, and 14 days after intervention. Moreover, 16S rRNA gene amplicon sequencing was used to analyze microbiota taxonomic composition. Results: The saliva microbiome differed significantly in response to the elimination diet (unweighted UniFrac: F = 1.72, R = 0.06, p < 0.005; weighted UniFrac: F = 7.62, R = 0.23, p-value = 0.0025) with a significant reduction in Firmicutes (p = 0.004) and a significant increase in Proteobacteria (p = 0.005). The juice intervention diets were also associated with changes in the saliva and cheek microbiota, particularly in the relative abundances of pro-inflammatory bacterial families, potentially due to the high sugar and low fiber intake of the juice-related products. Although no significant shifts in overall gut microbiota composition were observed, with either the elimination diet or the juice intervention diets, bacterial taxa associated with gut permeability, inflammation, and cognitive decline increased in relative abundance. Conclusions: These findings suggest that short-term juice consumption may negatively affect the microbiota. Full article

(This article belongs to the Section Prebiotics and Probiotics)

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28 pages, 2730 KiB

Open AccessEditor’s ChoiceReview

Minerals and Human Health: From Deficiency to Toxicity

by Mohammed S. Razzaque and Sunil J. Wimalawansa

Nutrients 2025, 17(3), 454; https://doi.org/10.3390/nu17030454 - 26 Jan 2025

Cited by 18 | Viewed by 11437

Abstract

Minerals are essential nutrients that play critical roles in human health by regulating various physiological functions. Examples include bone development, enzyme function, nerve signaling, and the immune response. Both the deficiencies and toxicities of minerals can have significant health implications. Deficiencies in macrominerals [...] Read more.

Minerals are essential nutrients that play critical roles in human health by regulating various physiological functions. Examples include bone development, enzyme function, nerve signaling, and the immune response. Both the deficiencies and toxicities of minerals can have significant health implications. Deficiencies in macrominerals such as calcium, magnesium, and phosphate can lead to osteoporosis (associated with falls and fractures), cardiovascular events, and neuromuscular dysfunction. Trace mineral deficiencies, such as iron and zinc. Selenium deficiency impairs oxygen transport, immune function, and antioxidant defenses, contributing to anemia, delaying wound healing, and increasing susceptibility to infectious diseases. Conversely, excessive intake of minerals can have severe health consequences. Hypercalcemia can cause kidney stones and cardiac arrhythmias as well as soft-tissue calcification, whereas excessive iron deposition can lead to oxidative stress and organ/tissue damage. Maintaining adequate mineral levels through a balanced diet, guided supplementation, and monitoring at-risk populations is essential for good health and preventing disorders related to deficiencies and toxicities. Public health interventions and education about dietary sources of minerals are critical for minimizing health risks and ensuring optimal well-being across populations. While a comprehensive analysis of all macro and micronutrients is beyond the scope of this article, we have chosen to focus on calcium, magnesium, and phosphate. We summarize the consequences of deficiency and the adverse events associated with the overconsumption of other minerals. Full article

(This article belongs to the Special Issue Mineral Nutrition on Human Health and Disease)

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16 pages, 1059 KiB

Open AccessEditor’s ChoiceSystematic Review

Effects of Chewing Gum on Satiety, Appetite Regulation, Energy Intake, and Weight Loss: A Systematic Review

by Claudia Jiménez-ten Hoevel, Elisabet Llauradó, Rosa M. Valls, Maria Besora-Moreno, Judit Queral, Rosa Solà and Anna Pedret

Nutrients 2025, 17(3), 435; https://doi.org/10.3390/nu17030435 - 25 Jan 2025

Cited by 2 | Viewed by 7208

Abstract

Background: New approaches for the management of obesity, a worldwide problem and a major determinant of disability and mortality, are needed. Mastication influences appetite and satiety mechanisms via actual food or sham feeding. However, the effect of mastication of chewing gum, a type [...] Read more.

Background: New approaches for the management of obesity, a worldwide problem and a major determinant of disability and mortality, are needed. Mastication influences appetite and satiety mechanisms via actual food or sham feeding. However, the effect of mastication of chewing gum, a type of sham feeding, on appetite regulation has not yet been elucidated. Objectives: Our aim was to evaluate the influence of chewing gum on appetite regulation, satiety, energy intake, and weight loss via randomized controlled Trials. Methods: This study was conducted in accordance with the 2020 PRISMA guidelines, and the protocol was registered in PROSPERO (CRD42023432699). Electronic databases MEDLINE^®/PubMed, Scopus, and Cochrane Central Register of Controlled Trials were searched from July 2023 to September 2024. The quality of each included study was assessed using the Cochrane risk of bias tool, RoB 2. Results: A total of eight articles with nine RCTs were included in this systematic review. Seven out of nine RCTs evaluated appetite regulation. Five out of seven RCTs reported a significant suppressing effect of hunger, three out of five RCTs reported a significant reduction in desire to eat, and three out of four reported a significant reduction in the desire to eat a sweet snack, all of them compared to the control group. However, the effects on satiety, energy intake, and weight loss are not conclusive. Conclusions: Chewing gum could be a promising non-pharmacological tool for obesity management through appetite regulation; however, further research, with sustained RCTs evaluating the sustained effects of gum chewing on appetite and weight management, is needed. Full article

(This article belongs to the Special Issue Featured Articles on Nutrition and Obesity Management (2nd Edition))

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21 pages, 1920 KiB

Open AccessEditor’s ChoiceReview

Effects of the Mediterranean Diet on the Components of Metabolic Syndrome Concerning the Cardiometabolic Risk

by Stefania Scaglione, Tiziana Di Chiara, Mario Daidone and Antonino Tuttolomondo

Nutrients 2025, 17(2), 358; https://doi.org/10.3390/nu17020358 - 20 Jan 2025

Cited by 7 | Viewed by 9615

Abstract

Metabolic syndrome is a cluster of risk factors, including abdominal obesity, insulin resistance, hypertension, dyslipidemia (intended as an increase in triglyceride levels and a reduction in HDL cholesterol levels), and elevated fasting glucose, that increase the risk of cardiovascular disease and type 2 [...] Read more.

Metabolic syndrome is a cluster of risk factors, including abdominal obesity, insulin resistance, hypertension, dyslipidemia (intended as an increase in triglyceride levels and a reduction in HDL cholesterol levels), and elevated fasting glucose, that increase the risk of cardiovascular disease and type 2 diabetes. With the rising prevalence of metabolic syndrome, effective dietary interventions are essential in reducing these health risks. The Mediterranean diet, rich in fruits, vegetables, whole grains, legumes, nuts, and olive oil and moderate in fish and poultry, has shown promise in addressing metabolic syndrome and its associated components. This diet’s anti-inflammatory and antioxidant properties, primarily due to its unsaturated fats, polyphenols, and fiber, have improved blood pressure, lipid levels, and insulin sensitivity. Adherence to the Mediterranean diet has been linked to reductions in central obesity and insulin resistance, both key elements in managing metabolic syndrome. Regarding lipid management, the Mediterranean diet lowers triglyceride levels and low-density lipoprotein (LDL) cholesterol while raising high-density lipoprotein (HDL) cholesterol, enhancing lipid profiles. It also helps regulate blood glucose levels, reducing the likelihood of developing type 2 diabetes. Additionally, the diet promotes weight loss and improves body composition, particularly by decreasing visceral fat, a primary driver of metabolic syndrome according to IDF classification. The Mediterranean diet offers a holistic approach to managing metabolic syndrome and reducing the risk of related chronic diseases. Its positive impact on metabolic health, combined with lifestyle changes like increased physical activity, provides a sustainable method for addressing the global burden of this syndrome. This review aimed to summarize the positive effects of the Mediterranean diet on the component of the metabolic syndrome with subsequent positive effects on cardiometabolic risk profile. Full article

(This article belongs to the Special Issue Mediterranean Diet and Metabolic Syndrome)

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29 pages, 2118 KiB

Open AccessEditor’s ChoiceReview

Dietary Interventions, Supplements, and Plant-Derived Compounds for Adjunct Vitiligo Management: A Review of the Literature

by Michael J. Diaz, Jasmine T. Tran, Drake Rose, Aria Wei, Deepak Lakshmipathy and Shari R. Lipner

Nutrients 2025, 17(2), 357; https://doi.org/10.3390/nu17020357 - 20 Jan 2025

Cited by 1 | Viewed by 6377

Abstract

Vitiligo is a chronic autoimmune pigmentation disorder shaped by a complex interplay of genetic predispositions and environmental triggers. While conventional therapies—phototherapy, corticosteroids, and immunosuppressants—can be effective, their benefits are often partial and temporary, with recurrence common once treatment stops. As such, there is [...] Read more.

Vitiligo is a chronic autoimmune pigmentation disorder shaped by a complex interplay of genetic predispositions and environmental triggers. While conventional therapies—phototherapy, corticosteroids, and immunosuppressants—can be effective, their benefits are often partial and temporary, with recurrence common once treatment stops. As such, there is increasing interest in exploring complementary approaches that may offer a more sustainable impact. Emerging evidence suggests that macronutrient and micronutrient-level changes could be beneficial for managing progression and, in some cases, facilitating repigmentation. Antioxidant-rich foods, such as apples, green tea, Indian gooseberry, onions, and peppers, may help mitigate oxidative stress, while inflammatory foods, such as gluten and high-phenol nuts and berries, may exacerbate the condition. Certain supplements, including high-dose vitamin D, vitamin C, vitamin E, and selenium, may enhance phototherapy outcomes. Omega-3 and other unsaturated fatty acids, in addition to prebiotics and probiotics, are under active investigation for their roles in gut health and immune regulation. Notably, plant-derived compounds, i.e., Ginkgo biloba, have demonstrated promise in promoting repigmentation and managing disease progression. However, it must be emphasized that these nutritional interventions remain exploratory, and more research is needed to establish their efficacy, safety, and optimal usage before they can be recommended as part of a standard treatment regimen. Full article

(This article belongs to the Special Issue Relationships between Dietary Factors and Inflammatory Skin Diseases)

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16 pages, 2855 KiB

Open AccessEditor’s ChoiceArticle

Egg Consumption and Mortality: A Prospective Cohort Study of Australian Community-Dwelling Older Adults

by Holly Wild, Danijela Gasevic, Robyn L. Woods, Joanne Ryan, Rory Wolfe, Yuquan Chen, Thara Govindaraju, John J. McNeil, Tracy McCaffrey, Lawrence J. Beilin, Dragan Ilic and Alice J. Owen

Nutrients 2025, 17(2), 323; https://doi.org/10.3390/nu17020323 - 17 Jan 2025

Cited by 1 | Viewed by 24673

Abstract

Background/Objectives: Egg consumption in adults has been linked with a modestly increased risk of all-cause and CVD mortality. However, evidence on adults aged 65 y+ is limited. The objective of this study was to investigate the association between egg intake and mortality in [...] Read more.

Background/Objectives: Egg consumption in adults has been linked with a modestly increased risk of all-cause and CVD mortality. However, evidence on adults aged 65 y+ is limited. The objective of this study was to investigate the association between egg intake and mortality in community-dwelling older adults. Methods: In this prospective cohort study, 8756 adults aged 70+ years, participants in the ASPirin in Reducing Events in the Elderly (ASPREE) Longitudinal Study of Older Persons, self-reported the frequency of their total egg intake: never/infrequently (rarely/never, 1–2 times/month), weekly (1–6 times/week), and daily (daily/several times per day). All-cause and cause-specific (cardiovascular disease [CVD] and cancer) mortality was established from at least two sources: medical records, death notices, next of kin, or death registry linkage. The association between egg intake and mortality was assessed using Cox proportional hazards regression analysis, adjusted for socio-demographic, health-related, and clinical factors and overall dietary quality. Results: Over the median 5.9-year follow-up period, a total of 1034 all-cause deaths (11.8%) were documented. A 29% lower risk of CVD mortality (HR (95% CI): 0.71 [0.54–0.92]) and a 17% (HR (95% CI): 0.83 [0.71–0.96]) lower risk of all-cause mortality were observed among those who consumed eggs weekly, compared to those who consumed eggs never/infrequently; no statistically significant association was observed for weekly consumption and cancer mortality. In contrast, compared to those that never or infrequently consumed eggs, daily consumption had slightly higher odds of mortality, though these results did not reach statistical significance. Conclusions: The consumption of eggs 1–6 times per week was associated with a lower risk of all-cause mortality and CVD mortality in community-dwelling adults aged 70 years and over. These findings may be important to inform the development of evidence-based guidelines for egg consumption. Full article

(This article belongs to the Special Issue Dietary Management and Nutritional Health for Age-Related Diseases)

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18 pages, 326 KiB

Open AccessEditor’s ChoiceReview

The Impact of Breakfast Consumption or Omission on Exercise Performance and Adaptations: A Narrative Review

by Matthew T. Stratton, Shelley L. Holden, Ray Davis and Austin T. Massengale

Nutrients 2025, 17(2), 300; https://doi.org/10.3390/nu17020300 - 15 Jan 2025

Viewed by 7055

Abstract

Background: Breakfast is often termed the most important meal of the day. However, its importance to acute and chronic adaptations to exercise is currently not well summarized throughout the literature. Methods: A narrative review of the experimental literature regarding breakfast consumption’s impact on [...] Read more.

Background: Breakfast is often termed the most important meal of the day. However, its importance to acute and chronic adaptations to exercise is currently not well summarized throughout the literature. Methods: A narrative review of the experimental literature regarding breakfast consumption’s impact on acute and chronic exercise performance and alterations in body composition prior to November 2024 was conducted. To be included in this review, the selected investigations needed to include some aspect of either endurance or resistance training performance and be conducted in humans. Results: These findings suggest that breakfast consumption may benefit acute long-duration (>60 min) but not short-duration (<60 min) morning endurance exercise. Evening time trial performance was consistently inhibited following breakfast omission despite the resumption of eating midday. No or minimal impact of breakfast consumption was found when examining acute morning or afternoon resistance training or the longitudinal adaptations to either resistance or endurance training. Favorable changes in body composition were often noted following the omission of breakfast. However, this was primarily driven by the concomitant reduced kilocalorie intake. Conclusions: Consuming breakfast may aid endurance athletes regularly performing exercise lasting >60 min in length. However, the morning meal’s impact on resistance training and changes in body composition appears to be minimal. Although, as the body of literature is limited, future investigations are needed to truly ascertain the dietary practice’s impact. Full article

(This article belongs to the Special Issue The Role of Nutrition in Exercise and Sports)

19 pages, 1716 KiB

Open AccessEditor’s ChoiceReview

Efficacy of N-Acetylcysteine in Polycystic Ovary Syndrome: Systematic Review and Meta-Analysis

by Isabel Viña, Juan R. Viña, Macarena Carranza and Gonzalo Mariscal

Nutrients 2025, 17(2), 284; https://doi.org/10.3390/nu17020284 - 14 Jan 2025

Cited by 3 | Viewed by 7143

Abstract

Background: Polycystic ovary syndrome (PCOS) is a common endocrine disorder that affects women of reproductive age and requires better treatment. N-acetylcysteine (NAC) is known to be beneficial under such conditions owing to its antioxidant potential and insulin-sensitizing properties. The effect of NAC [...] Read more.

Background: Polycystic ovary syndrome (PCOS) is a common endocrine disorder that affects women of reproductive age and requires better treatment. N-acetylcysteine (NAC) is known to be beneficial under such conditions owing to its antioxidant potential and insulin-sensitizing properties. The effect of NAC on the reproductive outcomes of PCOS patients was examined in this meta-analysis. Methods: In accordance with PRISMA standards, this meta-analysis included studies that compared N-acetylcysteine, metformin, clomiphene citrate, and a placebo in patients with POCS. The main indicators were follicular growth, endometrial thickness, and hormone level. The risk of bias was evaluated using the Cochrane ROB2 tool. Results: Twenty-two studies (n = 2515) were included. NAC was associated with a statistically significant increase in progesterone (SMD 0.95, 95% CI: 0.13–1.77, p = 0.02) and endometrial thickness (SMD 0.58, 95% CI: 0.10–1.06, p = 0.02) compared to the placebo and other drugs (SMD 0.71, 95% CI: 0.48–0.94, p < 0.0001). LH levels were significantly increased by NAC compared to metformin (SMD 0.67, 95% CI: 0.23–1.12, p = 0.003). However, no significant differences were observed in the estradiol, SHBG, or FSH levels. Conclusions: NAC had a major effect on progesterone, endometrial thickness, and LH levels in women with PCOS. Therefore, it may be a potential treatment option. Full article

(This article belongs to the Section Micronutrients and Human Health)

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18 pages, 571 KiB

Open AccessEditor’s ChoiceReview

Enhanced Vitamin C Delivery: A Systematic Literature Review Assessing the Efficacy and Safety of Alternative Supplement Forms in Healthy Adults

by Philip C. Calder, Richard B. Kreider and Diane L. McKay

Nutrients 2025, 17(2), 279; https://doi.org/10.3390/nu17020279 - 14 Jan 2025

Cited by 1 | Viewed by 10045

Abstract

Vitamin C is an antioxidant and is essential for immune function and infection resistance. Supplementation is necessary when a sufficient amount of vitamin C is not obtained through the diet. Alternative formulations of vitamin C may enhance its bioavailability and retention over traditional [...] Read more.

Vitamin C is an antioxidant and is essential for immune function and infection resistance. Supplementation is necessary when a sufficient amount of vitamin C is not obtained through the diet. Alternative formulations of vitamin C may enhance its bioavailability and retention over traditional ascorbic acid. This systematic review consolidates the evidence on this and the effects on immunity and infection. A systematic literature search was conducted in October 2024 in Embase and Medline, focused on healthy adults (Population); oral forms of liposomal-encapsulated ascorbic acid, liposomal-encapsulated lipid metabolite ascorbic acid, calcium ascorbate, slow-release ascorbic acid, or lipid metabolite ascorbic acid (Intervention); compared to placebo/others (Comparison); in terms of bioavailability, absorption, vitamin C concentration in plasma, serum, and leukocytes, and impacts on tolerability, immunity, and infection (Outcome); and included randomized or non-randomized controlled trials, single-arm trials, and observational studies (Study design). Thirteen studies were included, several evaluating calcium ascorbate in combination with vitamin C metabolites, including L-threonate, referred to here as Calcium ascorbate EC (Ester C^®; n = 7). No safety or tolerability concerns were noted with Calcium ascorbate EC vs. placebo or ascorbic acid. Calcium ascorbate EC showed better tolerability and fewer epigastric adverse events, improved quality of life, and induced favorable oxalate changes vs. ascorbic acid. Four studies reported leukocyte vitamin C concentration, some showing higher concentrations with Calcium ascorbate EC vs. ascorbic acid; seven reported more favorable plasma concentrations with the alternative forms over ascorbic acid or placebo; one reported higher serum vitamin C levels with vitamin C lipid metabolites than with Calcium ascorbate EC, calcium ascorbate, and ascorbic acid. No study reported retention in tissues. One study reported a favorable impact of Calcium ascorbate EC on immune parameters, and one found an association of Calcium ascorbate EC with fewer colds and a shorter duration of severe symptoms vs. placebo. Findings suggest that alternative vitamin C forms can improve leukocyte vitamin C, sometimes without affecting plasma levels. Most studies (77%) had a low risk of bias. In conclusion, the type and delivery modality of vitamin C can impact its bioavailability and functionality. Studies highlight the advantages of Calcium ascorbate EC over traditional ascorbic acid in terms of its tolerability and its potential to increase leukocyte vitamin C concentrations, crucial for immune function and protection against infection. However, further research is required to conclusively establish its effects on immune health. Full article

(This article belongs to the Special Issue Vitamin C: Challenges and Opportunities)

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15 pages, 1223 KiB

Open AccessEditor’s ChoiceSystematic Review

Relationship Between the Gut Microbiome, Tryptophan-Derived Metabolites, and Osteoarthritis-Related Pain: A Systematic Review with Meta-Analysis

by Erika Meléndez-Oliva, Oliver Martínez-Pozas, Pierluigi Sinatti, Carmen Martín Carreras-Presas, Juan Nicolás Cuenca-Zaldívar, Silvia Turroni and Eleuterio A. Sánchez Romero

Nutrients 2025, 17(2), 264; https://doi.org/10.3390/nu17020264 - 12 Jan 2025

Cited by 7 | Viewed by 4608

Abstract

Introduction: Osteoarthritis (OA) is the most prevalent form of arthritis and affects over 528 million people worldwide. Degenerative joint disease involves cartilage degradation, subchondral bone remodeling, and synovial inflammation, leading to chronic pain, stiffness, and impaired joint function. Initially regarded as a “wear [...] Read more.

Introduction: Osteoarthritis (OA) is the most prevalent form of arthritis and affects over 528 million people worldwide. Degenerative joint disease involves cartilage degradation, subchondral bone remodeling, and synovial inflammation, leading to chronic pain, stiffness, and impaired joint function. Initially regarded as a “wear and tear” condition associated with aging and mechanical stress, OA is now recognized as a multifaceted disease influenced by systemic factors such as metabolic syndrome, obesity, and chronic low-grade inflammation. Recent studies have focused on the gut-joint axis to investigate how the gut microbiome modulates inflammation and pain in OA. Materials and Methods: A systematic review was conducted following the PRISMA guidelines and was registered with PROSPERO (CRD42024556265). This review included studies involving adults with symptomatic OA and analyzed the relationship between the gut microbiome and OA-related pain. Randomized and non-randomized clinical trials, case reports, editorials, and pilot studies were excluded. Searches were performed in PubMed, Cochrane Library, and Web of Science without publication date restrictions, and filtered for “observational studies”. The study selection and data extraction were performed by two independent researchers, and the risk of bias was assessed using appropriate tools. Results: Five observational studies were included in the systematic review, and three were included in the meta-analysis. Two studies reported an association between different tryptophan metabolites and pain levels in patients with OA. Two other studies demonstrated a correlation between lipopolysaccharide levels and pain in OA. A fifth study confirmed the relationship between Streptococcus relative abundance of Streptococcus spp. and knee pain. These results were not supported by a meta-analysis, which found no significant association between the presence of pain in OA and the presence of bacilli of the genus Streptococcus or plasma markers of the tryptophan pathway. Conclusions: Current evidence indicates a potential link between gut microbiome dysbiosis and OA-related pain. However, methodological limitations preclude definitive conclusions. Further research using advanced techniques and larger cohorts is needed to validate and extend these findings and elucidate the underlying mechanisms. Targeted manipulation of the gut microbiome may be a valuable strategy for pain management in OA patients. Full article

(This article belongs to the Special Issue Association of the Microbiota with Neurodegenerative Diseases)

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24 pages, 540 KiB

Open AccessEditor’s ChoiceReview

Does Creatine Supplementation Enhance Performance in Active Females? A Systematic Review

by Ryan Tam, Lachlan Mitchell and Adrienne Forsyth

Nutrients 2025, 17(2), 238; https://doi.org/10.3390/nu17020238 - 10 Jan 2025

Viewed by 16057

Abstract

The use of creatine as a dietary supplement is widespread. However, its reported performance benefit has been largely demonstrated in male populations. The aim was to evaluate the effectiveness of creatine supplementation in improving exercise performance in active females. A secondary aim was [...] Read more.

The use of creatine as a dietary supplement is widespread. However, its reported performance benefit has been largely demonstrated in male populations. The aim was to evaluate the effectiveness of creatine supplementation in improving exercise performance in active females. A secondary aim was to appraise the quality of research in this area. Five databases were searched from the earliest record to July 2024. Eligible studies used supplemental creatine as an intervention with physically active female participants and reported an exercise performance-related outcome. Study quality was appraised using the Critical Appraisal Skills Program randomised controlled trials checklist with four additional items related to methodological considerations for research with active females. Performance outcomes were categorised as strength/power, anaerobic, or aerobic. Of the 10,563 records identified, 27 studies were included. Participant calibre ranged from recreationally active to elite. Creatine interventions ranged from five days to 12 weeks and included a range of dosage strategies. Compared to placebo, 3/11 studies showed an improvement in strength/power outcomes, 4/17 showed an improvement in anaerobic outcomes, and 1/5 showed an improvement in aerobic outcomes. Study quality varied, but methodological considerations for research with female athletes were poorly addressed by most studies. Although some benefits were reported, most studies showed no improvement in performance compared to placebo. The heterogeneity in participant characteristics, performance tests, creatine intervention, insufficient consideration of the unique physiological characteristics of females, and an overall small evidence base limits our understanding of how creatine supplementation influences physical performance in active females. Full article

(This article belongs to the Special Issue Nutrition and Supplementation Strategies to Enhance Resistance Training Adaptations)

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21 pages, 2529 KiB

Open AccessEditor’s ChoiceArticle

Increased Oxidative and Nitrative Stress and Decreased Sex Steroid Relaxation in a Vitamin D-Deficient Hyperandrogenic Rodent Model—And a Validation of the Polycystic Ovary Syndrome Model

by Réka Eszter Sziva, Réka Kollarics, Éva Pál, Bálint Bányai, Ágnes Korsós-Novák, Zoltán Fontányi, Péter Magyar, Anita Süli, György L. Nádasy, Nándor Ács, Eszter Mária Horváth, Leila Hadjadj and Szabolcs Várbíró

Nutrients 2025, 17(2), 201; https://doi.org/10.3390/nu17020201 - 7 Jan 2025

Cited by 1 | Viewed by 3701

Abstract

Background/Objectives: Both hyperandrogenism (HA) and vitamin D deficiency (VDD) can separately lead to impaired vascular reactivity and ovulatory dysfunction in fertile females. The aim was to examine the early interactions of these states in a rat model of PCOS. Methods: Four-week-old adolescent female [...] Read more.

Background/Objectives: Both hyperandrogenism (HA) and vitamin D deficiency (VDD) can separately lead to impaired vascular reactivity and ovulatory dysfunction in fertile females. The aim was to examine the early interactions of these states in a rat model of PCOS. Methods: Four-week-old adolescent female rats were divided into four groups: vitamin D (VD)-supplemented (n = 12); VD-supplemented and testosterone-treated (n = 12); VDD- (n = 11) and VDD-and-testosterone-treated (n = 11). Animals underwent transdermal testosterone treatment for 8 weeks. Target VD levels were achieved with oral VD supplementation and a VD-free diet. Estrous cycles were followed by vaginal smear, and quantitative histomorphometric measurements of the ovaries were also taken. In the 8th week, testosterone- and estrogen-induced relaxation of coronary arterioles was examined with pressure angiography. Estrogen receptor (ER) density and oxidative and nitrative stress parameters (Poly-(ADP-Ribose)-Polymerase and 3-nitrotyrosine) in the vessel wall were investigated with immunohistochemistry. Results: VDD caused impaired estrous cycles, and testosterone caused anovulatory cycles (the cycles were stopped at the diestrous phase). VDD combined with testosterone treatment resulted in reduced testosterone and estrogen vasorelaxation, lower ER density, and higher oxidative and nitrative stress in the vessel wall. Conclusions: PCOS with vitamin D deficiency may be associated with increased oxidative–nitrative stress in coronary arterioles. This oxidative and nitrative stress, potentially caused by hyperandrogenism and/or vitamin D deficiency, could impair estrogen-induced relaxation of the coronary arterioles, possibly by decreasing NO bioavailability and disrupting the estrogen-induced relaxation pathway. Full article

(This article belongs to the Special Issue Impact of Diet, Nutrition and Lifestyle on Reproductive Health)

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25 pages, 2004 KiB

Open AccessEditor’s ChoiceArticle

Exploring the Role of Glycine Metabolism in Coronary Artery Disease: Insights from Human Genetics and Mouse Models

by Subarna Biswas, James R. Hilser, Nicholas C. Woodward, Zeneng Wang, Janet Gukasyan, Ina Nemet, William S. Schwartzman, Pin Huang, Yi Han, Zachary Fouladian, Sarada Charugundla, Neal J. Spencer, Calvin Pan, W. H. Wilson Tang, Aldons J. Lusis, Stanley L. Hazen, Jaana A. Hartiala and Hooman Allayee

Nutrients 2025, 17(1), 198; https://doi.org/10.3390/nu17010198 - 6 Jan 2025

Viewed by 3464

Abstract

Background: Circulating glycine levels have been associated with reduced risk of coronary artery disease (CAD) in humans but these associations have not been observed in all studies. We evaluated whether the relationship between glycine levels and atherosclerosis was causal using genetic analyses in [...] Read more.

Background: Circulating glycine levels have been associated with reduced risk of coronary artery disease (CAD) in humans but these associations have not been observed in all studies. We evaluated whether the relationship between glycine levels and atherosclerosis was causal using genetic analyses in humans and feeding studies in mice. Methods: Serum glycine levels were evaluated for association with risk of CAD in the UK Biobank. Genetic determinants of glycine levels were identified through a genome-wide association study (GWAS) and used to evaluate the causal relationship between glycine and risk of CAD by Mendelian randomization (MR). A dietary supplementation study was carried out with atherosclerosis-prone apolipoprotein E deficient (ApoE^−/−) mice to determine the effects of increased circulating glycine levels on cardiometabolic traits and aortic lesion formation. Results: Among 105,718 UK Biobank subjects, elevated serum glycine levels were associated with significantly reduced risk of prevalent CAD (Quintile 5 vs. Quintile 1 OR = 0.76, 95% CI 0.67–0.87; p < 0.0001) and incident CAD (Quintile 5 vs. Quintile 1 HR = 0.70, 95% CI 0.65–0.77; p < 0.0001) after adjustment for age, sex, ethnicity, anti-hypertensive and lipid-lowering medications, blood pressure, kidney function, and diabetes. A GWAS meta-analysis with 230,947 subjects identified 61 loci for glycine levels, of which 26 were novel. MR analyses provided modest evidence that genetically elevated glycine levels were causally associated with reduced systolic blood pressure and risk of type 2 diabetes, but did not provide significant evidence for an association with decreased risk of CAD. Glycine supplementation in mice had no effects on cardiometabolic traits or atherosclerotic lesion development. Conclusions: While expanding the genetic architecture of glycine metabolism, MR analyses and in vivo feeding studies did not provide evidence that the clinical association of this amino acid with atherosclerosis represents a causal relationship. Full article

(This article belongs to the Section Proteins and Amino Acids)

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31 pages, 458 KiB

Open AccessEditor’s ChoiceReview

Common Questions and Misconceptions About Energy Drinks: What Does the Scientific Evidence Really Show?

by Jose Antonio, Brandi Antonio, Shawn M. Arent, Darren G. Candow, Guillermo Escalante, Cassandra Evans, Scott Forbes, David Fukuda, Maureen Gibbons, Patrick Harty, Andrew R. Jagim, Douglas S. Kalman, Chad M. Kerksick, Jennifer A. Kurtz, Joseph Lillis, Lonnie Lowery, Gianna F. Mastrofini, Scotty Mills, Michael Nelson, Flavia Pereira, Justin Roberts, Michael Sagner, Jeffrey Stout, Jaime Tartar and Adam Wells Show full author list Hide full author list

Nutrients 2025, 17(1), 67; https://doi.org/10.3390/nu17010067 - 27 Dec 2024

Cited by 2 | Viewed by 16779

Abstract

Energy drinks are a commonly consumed beverage, and studies suggest a possible performance-enhancing effect. A Google Scholar search using the keywords “energy drinks” and “exercise” yields numerous results, underscoring the voluminous research on this topic. However, there are questions regarding the effectiveness and [...] Read more.

Energy drinks are a commonly consumed beverage, and studies suggest a possible performance-enhancing effect. A Google Scholar search using the keywords “energy drinks” and “exercise” yields numerous results, underscoring the voluminous research on this topic. However, there are questions regarding the effectiveness and safety of energy drinks. These questions include, but are not limited to: (1) What are the main active ingredients in energy drinks? (2) Do energy drinks assist in weight management? (3) Do energy drinks enhance aerobic performance? (4) Do energy drinks enhance athletic speed? (5) Do energy drinks improve reaction time? (6) Do energy drinks enhance lean tissue mass? (7) Can energy drinks improve cognitive performance? (8) Does the acute consumption of energy drinks elevate resting energy expenditure? (9) Is there any evidence to suggest that energy drinks are more effective than an identical serving of caffeine alone? (10) Are there sex differences in the response to energy drink consumption? (11) Do energy drinks affect sleep or sleepiness? (12) Should pregnant women avoid energy drinks? (13) Do energy drinks adversely affect cardiovascular function? (14) Does consuming energy drinks cause brain damage? (15) What are other safety considerations regarding energy drinks? (16) Is there any evidence to suggest that energy drinks are more effective than an identical serving of caffeine alone? (17) If caffeine is the main active ingredient in energy drinks and coffee, why is there a discrepancy in the adverse events reported for each? To address these questions, we performed an evidence-based scientific evaluation of the literature on energy drink supplementation. Full article

(This article belongs to the Section Sports Nutrition)

24 pages, 1038 KiB

Open AccessEditor’s ChoiceReview

Dietary and Nutritional Interventions for the Management of Endometriosis

by Nour Abulughod, Stefanie Valakas and Fatima El-Assaad

Nutrients 2024, 16(23), 3988; https://doi.org/10.3390/nu16233988 - 21 Nov 2024

Cited by 3 | Viewed by 10470

Abstract

Endometriosis is a chronic, complex, systemic inflammatory condition that impacts approximately 190 million girls and women worldwide, significantly impacting their quality of life. The effective management of endometriosis requires a multi-disciplinary and holistic approach, one that includes surgical and medical management, such as [...] Read more.

Endometriosis is a chronic, complex, systemic inflammatory condition that impacts approximately 190 million girls and women worldwide, significantly impacting their quality of life. The effective management of endometriosis requires a multi-disciplinary and holistic approach, one that includes surgical and medical management, such as a laparoscopy and a chronic medical management plan, as well as dietary, nutritional, and lifestyle adjunct interventions, such as pelvic pain physiotherapy and acupuncture. There is growing evidence to support the role of dietary and nutritional interventions in the adjunct management of endometriosis-related pain and gastrointestinal symptoms. However, the implementation of these interventions is often not regulated, as patients with endometriosis often adopt self-management strategies. Diet and nutrition can modulate key players integral to the pathophysiology of endometriosis, such as, but not limited to, inflammation, estrogen, and the microbiome. However, it is unclear as to whether diet plays a role in the prevention or the onset of endometriosis. In this review, we discuss three key players in the pathogenesis of endometriosis—inflammation, estrogen, and the microbiome—and we summarize how diet and nutrition can influence their mechanisms, and consequently, the progression and manifestation of endometriosis. There is a major need for evidence-based, non-invasive adjunct management of this debilitating disease, and diet and nutritional interventions may be suitable. Full article

(This article belongs to the Section Nutrition in Women)

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26 pages, 1512 KiB

Open AccessEditor’s ChoiceReview

Breastfeeding Beyond Six Months: Evidence of Child Health Benefits

by Anita Froń and Magdalena Orczyk-Pawiłowicz

Nutrients 2024, 16(22), 3891; https://doi.org/10.3390/nu16223891 - 14 Nov 2024

Cited by 8 | Viewed by 10912

Abstract

Breastfeeding is globally recognized as the optimal method of infant nutrition, offering health benefits for both the child and the mother, making it a public health priority. However, the potential advantages of breastfeeding extend well beyond initial months. Breast milk adapts to the [...] Read more.

Breastfeeding is globally recognized as the optimal method of infant nutrition, offering health benefits for both the child and the mother, making it a public health priority. However, the potential advantages of breastfeeding extend well beyond initial months. Breast milk adapts to the evolving needs of the growing infant, and its immunological, microbiological, and biochemical properties have been associated with enhanced protection against infections and chronic diseases, improved growth and development, and lower rates of hospitalization and mortality. This review explores the evidence supporting the continuation of breastfeeding beyond six months. More meticulous studies employing consistent methodologies and addressing confounders are essential. This will enable a more accurate determination of the extent and mechanisms of the positive impact of prolonged breastfeeding and allow for the implementation of effective public health strategies. Full article

(This article belongs to the Special Issue Advances in Infant and Pediatric Feeding and Nutrition)

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31 pages, 3064 KiB

Open AccessEditor’s ChoiceReview

Unraveling the Gut Microbiota: Implications for Precision Nutrition and Personalized Medicine

by Alessio Abeltino, Duaa Hatem, Cassandra Serantoni, Alessia Riente, Michele Maria De Giulio, Marco De Spirito, Flavio De Maio and Giuseppe Maulucci

Nutrients 2024, 16(22), 3806; https://doi.org/10.3390/nu16223806 - 6 Nov 2024

Cited by 23 | Viewed by 8192

Abstract

Recent studies have shown a growing interest in the complex relationship between the human gut microbiota, metabolism, and overall health. This review aims to explore the gut microbiota–host association, focusing on its implications for precision nutrition and personalized medicine. The objective is to [...] Read more.

Recent studies have shown a growing interest in the complex relationship between the human gut microbiota, metabolism, and overall health. This review aims to explore the gut microbiota–host association, focusing on its implications for precision nutrition and personalized medicine. The objective is to highlight how gut microbiota modulate metabolic and immune functions, contributing to disease susceptibility and wellbeing. The review synthesizes recent research findings, analyzing key studies on the influence of gut microbiota on lipid and carbohydrate metabolism, intestinal health, neurobehavioral regulation, and endocrine signaling. Data were drawn from both experimental and clinical trials examining microbiota–host interactions relevant to precision nutrition. Our findings highlight the essential role of gut microbiota-derived metabolites in regulating host metabolism, including lipid and glucose pathways. These metabolites have been found to influence immune responses and gut barrier integrity. Additionally, the microbiota impacts broader physiological processes, including neuroendocrine regulation, which could be crucial for dietary interventions. Therefore, understanding the molecular mechanisms of dietary–microbiota–host interactions is pivotal for advancing personalized nutrition strategies. Tailored dietary recommendations based on individual gut microbiota compositions hold promise for improving health outcomes, potentially revolutionizing future healthcare approaches across diverse populations. Full article

(This article belongs to the Special Issue The Role of Microbiota in Nutrition and Diet: An Ever-Evolving Relationship)

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62 pages, 4356 KiB

Open AccessEditor’s ChoiceReview

Potential Strategies for Overcoming Drug Resistance Pathways Using Propolis and Its Polyphenolic/Flavonoid Compounds in Combination with Chemotherapy and Radiotherapy

by Nada Oršolić and Maja Jazvinšćak Jembrek

Nutrients 2024, 16(21), 3741; https://doi.org/10.3390/nu16213741 - 31 Oct 2024

Cited by 13 | Viewed by 3715

Abstract

Conventional cancer treatments include surgical resection, chemotherapy, hyperthermia, immunotherapy, hormone therapy, and locally targeted therapies such as radiation therapy. Standard cancer therapies often require the use of multiple agents, which can activate nuclear factor kappa B (NF-κB) in tumor cells, leading to reduced [...] Read more.

Conventional cancer treatments include surgical resection, chemotherapy, hyperthermia, immunotherapy, hormone therapy, and locally targeted therapies such as radiation therapy. Standard cancer therapies often require the use of multiple agents, which can activate nuclear factor kappa B (NF-κB) in tumor cells, leading to reduced cell death and increased drug resistance. Moreover, the use of multiple agents also contributes to added toxicity, resulting in poor treatment outcomes. Cancer cells gradually develop resistance to almost all chemotherapeutics through various mechanisms, such as drug efflux, alterations in drug metabolism and transport, changes in signal transduction pathways, enhanced DNA repair capacity, evasion of apoptosis, increased mutations, reactivation of drug targets, interaction with the cancer microenvironment, cancer cell-stroma interactions, epithelial–mesenchymal transition (EMT)-mediated chemoresistance, epigenetic modifications, metabolic alterations, and the effect of cancer stem cells (CSCs). Developing new strategies to improve chemotherapy sensitivity while minimizing side effects is essential for achieving better therapeutic outcomes and enhancing patients’ quality of life. One promising approach involves combining conventional cancer treatments with propolis and its flavonoids. These natural compounds may enhance tumor response to treatment while reducing toxicity. Propolis and its components can sensitize cancer cells to chemotherapeutic agents, likely by inhibiting NF-κB activation, reprogramming tumor-associated macrophages (TAMs; an M2-like phenotype), and thereby reducing the release of matrix metalloproteinase (MMP)-9, cytokines, chemokines, and the vascular endothelial growth factor (VEGF). By reducing TAMs, propolis and its components may also overcome EMT-mediated chemoresistance, disrupt the crosstalk between macrophages and CSCs, inhibit the maintenance of stemness, and reverse acquired immunosuppression, thus promoting an antitumor response mediated by cytotoxic T-cells. This review highlights the potential of flavonoids to modulate the responsiveness of cancer to conventional treatment modalities. The evidence suggests that novel therapeutic strategies incorporating flavonoids could be developed to improve treatment outcomes. The positive effects of combining propolis with chemotherapeutics include reduced cytotoxicity to peripheral blood leukocytes, liver, and kidney cells. Therefore, polyphenolic/flavonoid components may hold potential for use in combination with chemotherapeutic agents in the clinical treatment of various types of cancers. Full article

(This article belongs to the Special Issue Effects of Phytochemicals on Human Health)

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26 pages, 738 KiB

Open AccessEditor’s ChoiceReview

Vitamin D Supplementation: Shedding Light on the Role of the Sunshine Vitamin in the Prevention and Management of Type 2 Diabetes and Its Complications

by Dimitra Vasdeki, Georgios Tsamos, Evangelos Dimakakos, Vasileios Patriarcheas, Theocharis Koufakis, Kalliopi Kotsa, Armand Cholewka and Agata Stanek

Nutrients 2024, 16(21), 3651; https://doi.org/10.3390/nu16213651 - 26 Oct 2024

Cited by 12 | Viewed by 8643

Abstract

As the incidence of type 2 diabetes mellitus (T2DM) continues to increase globally, researchers are keen to investigate various interventions to mitigate its impact. Among these, vitamin D supplementation has attracted significant attention due to its influence on insulin secretion from the pancreas [...] Read more.

As the incidence of type 2 diabetes mellitus (T2DM) continues to increase globally, researchers are keen to investigate various interventions to mitigate its impact. Among these, vitamin D supplementation has attracted significant attention due to its influence on insulin secretion from the pancreas and insulin receptors in body cells. A substantial body of evidence indicates that vitamin D supplementation can reduce low-grade inflammation, a critical factor in developing insulin resistance. In addition, vitamin D aids in sustaining low resting concentrations of reactive oxygen species and free radicals, normalizes Ca²⁺ signaling, diminishes the expression of cytokines that are pro-inflammatory, and enhances the production of cytokines that are anti-inflammatory. This review discusses the effects of vitamin D on the glycemic control of individuals with T2DM and evaluates the impact of vitamin D supplementation on glycemic markers in this population. The investigation employs a comprehensive analysis of the existing literature with a special focus on recent studies published in the past decade. Based on the findings in the literature, it can be concluded that vitamin D supplementation alongside anti-diabetic medications may enhance glycemic control and potentially reduce the risk of diabetic complications. The evidence supports the notion that vitamin D supplementation can be a valuable addition to pharmacological agents for the management of T2DM, potentially enhancing glycemic control and overall health outcomes in affected individuals. Full article

(This article belongs to the Special Issue Diabetes Mellitus and Nutritional Supplements)

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13 pages, 1887 KiB

Open AccessEditor’s ChoiceReview

Health Benefits Beyond the Scale: The Role of Diet and Nutrition During Weight Loss Programmes

by Francisca Contreras, Werd Al-Najim and Carel W. le Roux

Nutrients 2024, 16(21), 3585; https://doi.org/10.3390/nu16213585 - 22 Oct 2024

Cited by 9 | Viewed by 18651

Abstract

Introduction: Obesity management strategies such as caloric restriction, very-low-calorie diets (VLCDs), and meal replacements can lead to moderate short-term weight loss. However, many patients face significant challenges in maintaining these results. Personalized interventions, including behavioral counseling and physical activity, have been shown to [...] Read more.

Introduction: Obesity management strategies such as caloric restriction, very-low-calorie diets (VLCDs), and meal replacements can lead to moderate short-term weight loss. However, many patients face significant challenges in maintaining these results. Personalized interventions, including behavioral counseling and physical activity, have been shown to improve long-term adherence and success. Current clinical guidelines emphasize the pivotal role of dietitians in enhancing patient outcomes through nutritional therapy. When combined with pharmacotherapy and bariatric surgery, the focus shifts from mere weight loss to broader health improvements. Methods: This review explores the evolving role of dietitians in obesity management, advocating for a shift from a weight-centric approach to a more holistic model that prioritizes overall health gains. Key areas of interest include dietetic interventions’ impact on metabolic health, cardiovascular function, gut microbiome balance, inflammation, and psychological well-being. Results: Dietetic interventions have been shown to provide significant health improvements beyond weight loss. These include enhanced metabolic and cardiovascular health, better gut microbiome balance, reduced inflammation, improved sleep quality, mental well-being, and overall quality of life. By focusing on non-scale victories such as improved insulin sensitivity, lipid profiles, and mental health, dietitians play a crucial role in driving long-term success in obesity management. These outcomes highlight the need to shift the focus from short-term weight loss to a more comprehensive view of health gains. Conclusions: The role of dietitians in obesity management is expanding to encompass a more comprehensive and individualized approach. Moving beyond a focus on weight reduction, this paradigm promotes long-term, patient-centered strategies that address the multifactorial nature of obesity. By combining dietary changes with regular physical activity and behavioral support, dietitians can contribute to sustained health improvements, treating obesity as a chronic, complex disease. Full article

(This article belongs to the Special Issue Diet and Nutrition Approaches in Obesity Treatment)

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23 pages, 1538 KiB

Open AccessEditor’s ChoiceReview

Exploring the Interplay of Genetics and Nutrition in the Rising Epidemic of Obesity and Metabolic Diseases

by Sylwia Górczyńska-Kosiorz, Matylda Kosiorz and Sylwia Dzięgielewska-Gęsiak

Nutrients 2024, 16(20), 3562; https://doi.org/10.3390/nu16203562 - 21 Oct 2024

Cited by 18 | Viewed by 8488

Abstract

Background: Obesity has become a significant global health issue. This multifaceted condition is influenced by genetic, environmental, and lifestyle factors, significantly influenced by nutrition. Aim: The study’s objective is to elucidate the relationship between obesity-related genes, nutrient intake, and the development of [...] Read more.

Background: Obesity has become a significant global health issue. This multifaceted condition is influenced by genetic, environmental, and lifestyle factors, significantly influenced by nutrition. Aim: The study’s objective is to elucidate the relationship between obesity-related genes, nutrient intake, and the development of obesity and the importance of other metabolic diseases. Methods: A comprehensive literature review spanning the past two decades was conducted to analyze the contributions of genetic variants—including FTO, MC4R, and LEPR—and their associations with dietary habits, highlighting how specific nutrients affect gene expression and obesity risk and how the coexistence of metabolic diseases such as type 2 diabetes and osteoporosis may modulate these factors. Moreover, the role of epigenetic factors, such as dietary patterns that encourage the development of obesity, was explored. Discussion and Conclusions: By understanding the intricate relationships among genetics, nutrients, and obesity development, this study highlights the importance of personalized dietary strategies in managing obesity. Overall, an integrated approach that considers genetic predispositions alongside environmental influences is essential for developing effective prevention and treatment methodologies, ultimately contributing to better health outcomes in diverse populations. Full article

(This article belongs to the Section Nutrigenetics and Nutrigenomics)

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24 pages, 1357 KiB

Open AccessEditor’s ChoiceSystematic Review

Supplementation and Mitigating Cognitive Decline in Older Adults With or Without Mild Cognitive Impairment or Dementia: A Systematic Review

by Qi Fu, Jill DeJager and Elizabeth M. Gardner

Nutrients 2024, 16(20), 3567; https://doi.org/10.3390/nu16203567 - 21 Oct 2024

Cited by 4 | Viewed by 12208

Abstract

This systematic literature review aims to answer the question of how micronutrients might influence the development and progression of dementia. In the present work, we focused on an overview of an updated review of relevant literature published in the last two decades. This [...] Read more.

This systematic literature review aims to answer the question of how micronutrients might influence the development and progression of dementia. In the present work, we focused on an overview of an updated review of relevant literature published in the last two decades. This review aims to delineate the relationship between micronutrient supplementation and cognitive decline in older subjects. In carrying out this review, we followed PRISMA, and our literature search was performed on PubMed. This systematic review includes only primary studies that have investigated the efficacy of nutritional interventions for the prevention of dementia and improvement of cognitive function in subjects aged 65 years or older with normal cognition, mild cognitive impairment (MCI), or Alzheimer’s disease (AD). A gross heterogeneity of studies forbids the possibility of a direct comparison of the results. A review of the inclusion criteria and restrictions has been conducted to check the validity and reliability of the results. In this review, thirty-three primary studies were included. Results have shown that supplementation with vitamin D, probiotics, and PUFAs would most likely reduce cognitive decline, dementia, or AD compared with vitamins A, B, C, and E, which were seen to be relatively ineffective. Of note, when considering vitamin B supplementation, positive effects were only observed in non-aspirin users having high ω-3 fatty acid (ω-3 FA) plasma levels. In some cases, however, there were genotypic differences in subjects in response to vitamin B supplementation. Full article

(This article belongs to the Section Geriatric Nutrition)

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18 pages, 1861 KiB

Open AccessEditor’s ChoiceArticle

A One-Week Elderberry Juice Intervention Augments the Fecal Microbiota and Suggests Improvement in Glucose Tolerance and Fat Oxidation in a Randomized Controlled Trial

by Christy Teets, Nancy Ghanem, Guoying Ma, Jagrani Minj, Penelope Perkins-Veazie, Sarah A. Johnson, Andrea J. Etter, Franck G. Carbonero and Patrick M. Solverson

Nutrients 2024, 16(20), 3555; https://doi.org/10.3390/nu16203555 - 20 Oct 2024

Cited by 5 | Viewed by 20850

Abstract

Obesity is a costly and ongoing health complication in the United States and globally. Bioactive-rich foods, especially those providing polyphenols, represent an emerging and attractive strategy to address this issue. Berry-derived anthocyanins and their metabolites are of particular interest for their bioactive effects, [...] Read more.

Obesity is a costly and ongoing health complication in the United States and globally. Bioactive-rich foods, especially those providing polyphenols, represent an emerging and attractive strategy to address this issue. Berry-derived anthocyanins and their metabolites are of particular interest for their bioactive effects, including weight maintenance and protection from metabolic aberrations. Earlier findings from small clinical trials suggest modulation of substrate oxidation and glucose tolerance with mediation of prospective benefits attributable to the gut microbiota, but mixed results suggest appropriate anthocyanin dosing poses a challenge. The objective of this randomized, placebo-controlled study was to determine if anthocyanin-dense elderberry juice (EBJ) reproduces glucoregulatory and substrate oxidation effects observed with other berries and if this is mediated by the gut microbiota. Overweight or obese adults (BMI > 25 kg/m²) without chronic illnesses were randomized to a 5-week crossover study protocol with two 1-week periods of twice-daily EBJ or placebo (PL) separated by a washout period. Each treatment period included 4 days of controlled feeding with a 40% fat diet to allow for comparison of measurements in fecal microbiota, meal tolerance testing (MTT), and indirect calorimetry between test beverages. Eighteen study volunteers completed the study. At the phylum level, EBJ significantly increased Firmicutes and Actinobacteria, and decreased Bacteroidetes. At the genus level, EBJ increased Faecalibacterium, Ruminococcaceae, and Bifidobacterium and decreased Bacteroides and lactic acid-producing bacteria, indicating a positive response to EBJ. Supporting the changes to the microbiota, the EBJ treatment significantly reduced blood glucose following the MTT. Fat oxidation also increased significantly both during the MTT and 30 min of moderate physical activity with the EBJ treatment. Our findings confirm the bioactivity of EBJ-sourced anthocyanins on outcomes related to gut health and obesity. Follow-up investigation is needed to confirm our findings and to test for longer durations. Full article

(This article belongs to the Special Issue Dietary Intake of Phytochemicals, Gut Microbiota and Appetite Control)

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