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Microbiology Research, Volume 17, Issue 1

2026 January - 28 articles

Cover Story: Microbial steroids were long overshadowed by plant and animal counterparts, yet fungi, bacteria, actinomycetes and microalgae produce an extraordinary variety of steroidal metabolites. These compounds display unusual carbon frameworks, extensive oxygenation, heteroatom incorporation and unique biosynthetic transformations that place them in distinct regions of chemical space. Microbial steroids arise from canonical sterol pathways combined with innovative enzymatic reactions, yielding rearranged, polyoxygenated, sulfated or halogenated structures. Biologically, they exhibit anticancer, anti-inflammatory, antimicrobial, antiviral and signaling activities, reflecting vital ecological roles and pharmacological potential. Advances in genome mining, metabolomics and computational prediction now position them as a rich and underexplored source of novel bioactive scaffolds for drug discovery. View this paper
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Articles (28)

  • Article
  • Open Access
197 Views
16 Pages

Monoxenic Root Organ Culture Enables High-Yield Production of Viable Indigenous Rhizophagus irregularis Inoculum for Arid Oasis Agroecosystems

  • Elmostafa Gagou,
  • Hanae El Yeznasni,
  • Wissame Chafai,
  • Khadija Chakroune,
  • Mahmoud Abbas,
  • Touria Lamkami,
  • Mondher El Jaziri and
  • Abdelkader Hakkou

Arbuscular mycorrhizal fungi (AMF) play a pivotal role in plant adaptation to arid ecosystems, yet their widespread agricultural use is constrained by the scarcity of high-quality, locally adapted inoculum. This study established a reliable monoxenic...

  • Article
  • Open Access
311 Views
16 Pages

Genomic Relationship Between High-Risk Pseudomonas aeruginosa Clone ST244 Serotypes O5 and O12 from Southeastern Brazil

  • Kayo Bianco,
  • Thereza Cristina da Costa Vianna,
  • Samara Santanna de Oliveira,
  • Kaylanne Montenegro,
  • Claudia Flores,
  • Ana Paula Alves do Nascimento,
  • Alexander Machado Cardoso and
  • Maysa Mandetta Clementino

Pseudomonas aeruginosa is an opportunistic pathogen commonly associated with nosocomial infections and environmental dissemination. Among its high-risk clones, ST244 is notable for its global distribution and distinctive genomic traits. This study re...

  • Article
  • Open Access
342 Views
13 Pages

Cosmetic Wipe Sample Preparation for Microbiological Analysis—Single Laboratory Validation Study

  • Nadine Yossa,
  • Roma Adu Osei,
  • Travis Canida,
  • Anna Laasri,
  • Qing Jin,
  • Pascal Iraola,
  • Thomas Hammack,
  • Mei-Chiung Jo Huang,
  • Goran Periz and
  • Rachel Binet
  • + 1 author

Cosmetic wipes are made for multiple functions, baby care, hand washing, feminine and personal cleansing, removing makeup, and applying products such as deodorants and sunless tanners among other uses. Despite the presence of preservatives, cosmetic...

  • Case Report
  • Open Access
366 Views
18 Pages

Preliminary Assessment of Cow-Derived Fermented Product (CDFP) Effects on the Human Gut Microbiome: A Single-Subject Case Study

  • Niyati Desai,
  • Nilam Vaghamshi,
  • Komal Antaliya,
  • Ashaka Vansia,
  • Arpan Tapaniya,
  • Anjana Ghelani,
  • Rajesh Chaudhari,
  • Rajesh Patel,
  • Pravin Dudhagara and
  • Douglas J. H. Shyu

Cow’s milk, urine, dung, ghee, and curd possess significant medicinal value in Ayurveda and have been integral to traditional Indian clinical practices for centuries. The cow-derived fermented product (CDFP), a formulation rooted in Ayurvedic t...

  • Article
  • Open Access
251 Views
19 Pages

To address the challenges of low land use efficiency, soil degradation, and high management costs in Ilex asprella cultivation, this study established an I. asprellaGrona styracifolia intercropping system and systematically evaluated its effec...

  • Article
  • Open Access
263 Views
17 Pages

Vagococcus species have been isolated from diverse environments, including aquatic, terrestrial, food-associated, and clinical sources; however, plant- and flower-associated representatives remain poorly characterized at the genomic level. In this st...

  • Review
  • Open Access
436 Views
13 Pages

Regulatory Mechanisms of Zinc on Bacterial Antibiotic Resistance and Virulence in a One Health Context

  • Yang Wang,
  • Yue Li,
  • Jingyi Wu,
  • Mengge Shen,
  • Aoqi Zhan,
  • Yuxin Wang and
  • Baobao Liu

Zinc (Zn), a ubiquitous environmental transition metal primarily existing as Zinc ions (Zn2+), plays a critical role in various biological processes. Its extensive application in agriculture, industry, and healthcare has led to significant environmen...

  • Article
  • Open Access
387 Views
15 Pages

Seasonal Surveillance of Urban Water Quality in Southern Brazil Reveals Persistent Carbapenem Resistance Genes Despite Compliance with Bacteriological Standards

  • Laura Haleva,
  • Tiane Martin de Moura,
  • Luciana Costa Teixeira,
  • Horst Mitteregger Júnior,
  • Evgeni Evgeniev Gabev,
  • Adriana Ambrosini da Silveira and
  • Fabrício Souza Campos

Quality control of drinking water is essential for safeguarding public health, particularly in densely populated urban environments. Environmental microbiological monitoring can complement conventional surveillance by providing deeper insights into t...

  • Article
  • Open Access
354 Views
22 Pages

A One Health Perspective: Occurrence Study of Carbapenem-Resistant Bacteria and Other Emerging Pathogens from Recycled Wastewater Used in Agriculture

  • Maria Martínez-Ruiz,
  • Miriam Hernández-Porto,
  • Cintia Hernández-Sánchez,
  • Ángeles Arias,
  • José Carlos de Gracia,
  • Adolfo Perdomo-González,
  • Raquel Pérez-Reverón and
  • Francisco Javier Díaz Peña

Recycled wastewater is vital for the circular economy, especially on water-scarce islands. This study explored the presence of Carbapenem-Resistant Enterobacterales and other emerging pathogens in irrigation water on four Canarian Islands, applying a...

  • Article
  • Open Access
362 Views
14 Pages

Genetic Characterization of Avian Influenza Virus A (H1N1) Isolated from a Fieldfare Turdus pilaris in Ukraine

  • Alla Mironenko,
  • Nataliia Muzyka,
  • Nataliia Teteriuk,
  • Larysa Radchenko,
  • Anastasia Popova,
  • Jonas Waldenström and
  • Denys Muzyka

Avian influenza viruses are predominantly associated with waterfowl and shorebirds, and are rarely detected in other avian hosts in nature. In 2021, an H1N1 virus was isolated from a Fieldfare Turdus pilaris in Zaporizhzhia Oblast, Ukraine. A phyloge...

  • Review
  • Open Access
627 Views
32 Pages

Bacterial Bovine Respiratory Disease: A Comprehensive Review of Etiology, Pathogenesis and Management Strategies

  • Chiara Storoni,
  • Silvia Preziuso,
  • Anna-Rita Attili,
  • Yubao Li and
  • Vincenzo Cuteri

Bovine Respiratory Disease (BRD) represents one of the largest causes of economic loss and animal morbidity in the global cattle industry, second only to neonatal diarrhea. Its etiology is complex, originating from a multifactorial combination of hos...

  • Article
  • Open Access
414 Views
14 Pages

Resistance Patterns in Gram-Negative Bacilli Isolated in a Secondary Care Hospital: A Therapeutic Challenge in Western Mexico

  • César Ricardo Cortez-Álvarez,
  • Benjamín de Jesús Gutiérrez-García,
  • Pablo Ulises Romero-Mendoza,
  • María del Rosario Cabral-Medina,
  • Monserratt Abud-Gonzalez,
  • Susana Olivia Guerra-Martínez,
  • Livier Amalia Gutiérrez-Morales,
  • María Luisa Muñoz-Almaguer,
  • Santiago José Guevara-Martínez and
  • Martin Zermeño-Ruiz
  • + 2 authors

Antimicrobial resistance (AMR) continues to represent a significant global public health concern. Gram-negative bacilli (GNB) are the primary causative agents of severe nosocomial infections and possess a notable capacity to develop resistance mechan...

  • Article
  • Open Access
443 Views
17 Pages

Heat-Assisted Extraction and Bioactivity Evaluation of a Dinactin-Associated Compound from Streptomyces UP Strains

  • Grissana Pook-In,
  • Somsak Tammawong,
  • Chorpaka Phuangsri,
  • Khwanla Seansupa,
  • Sontaya Sookying,
  • Tomoko Takahashi and
  • Anchalee Rawangkan

Streptomyces is a versatile genus widely used in drug production and biotechnological applications. This study aimed to identify and characterize bioactive compounds produced by Streptomyces UP-AC4 and UP-3.2 strains and evaluate their antibacterial...

  • Review
  • Open Access
480 Views
23 Pages

Microbial Steroids: Novel Frameworks and Bioactivity Profiles

  • Valery M. Dembitsky and
  • Alexander O. Terent’ev

Microorganisms have emerged as prolific and versatile producers of steroidal natural products, displaying a remarkable capacity for structural diversification that extends far beyond classical sterol frameworks. This review critically examines steroi...

  • Article
  • Open Access
386 Views
17 Pages

Exploring Arid Soils as a Source of Bacillus thuringiensis Biocontrol Agents Active Against Dipteran and Lepidopteran Larvae

  • Kareem Badr,
  • Fatima Al-Maadeed,
  • Alaa Azouz,
  • Zahoor Ul Hassan,
  • Quirico Migheli and
  • Samir Jaoua

Microbial communities found in arid environments often exhibit unique genetic and metabolic adaptations that enable them to synthesize potent bioactive compounds. Bacillus thuringiensis (Bt) is widely recognized for its biocontrol potential against v...

  • Article
  • Open Access
363 Views
10 Pages

Salmonella enterica from low-moisture food has been found to have a higher thermal tolerance than from high-moisture food. However, the molecular mechanism underlying the association of thermal tolerance of this pathogen with low-moisture foods, such...

  • Article
  • Open Access
431 Views
18 Pages

Streptococcus pneumoniae (S. pneumoniae) is responsible for a wide range of infections. The aim of this study was to investigate the clonal diversity of S. pneumoniae in thirteen Arab countries. Multi-Locus Sequence Typing (MLST) data were extracted...

  • Article
  • Open Access
548 Views
18 Pages

Urinalysis and Antimicrobial Susceptibility of Bacteria Isolated from Urine of Dogs and Cats in Poland in 2023: Associations Between Urine Parameters and Bacteriuria

  • Dawid Jańczak,
  • Piotr Górecki,
  • Natalia Skrzypek,
  • Dominika Sobkiewicz,
  • Magda Paczocha,
  • Aleksander Chrzanowski,
  • Aleksandra Kornelia Maj,
  • Rafał Stryjek,
  • Aleksandra Anna Zasada and
  • Anna Golke

Bacterial urinary tract infections (UTIs) are common in dogs and cats. This study examined the correlations between routine urinalysis and culture-confirmed infections and described the etiologic agents and antimicrobial susceptibility to support ste...

  • Review
  • Open Access
593 Views
28 Pages

Mapping Research on Microbial Remediation of Metals in Soil (2020–2025)

  • Aziza Usmonkulova,
  • Massimo Pugliese,
  • Mukhiddin Juliev,
  • Ilkhom Khalilov,
  • Nafosat Kurbonova,
  • Nigora Tillyaxodjayeva,
  • Rixsiniso Karimova,
  • Wei Liu,
  • Feruza Khalilova and
  • Oysha Jabborova

This study involved a systematic literature review using bibliometric analysis to examine the evolution and current trends of Biological Remediation studies. The bibliometric analysis was used for the descriptive, intellectual, social, and conceptual...

  • Article
  • Open Access
1 Citations
702 Views
12 Pages

Gamsia batmanii sp. nov. Isolated from a Common Bent-Wing Bat and the Review of the Genus Gamsia

  • Žejko Savković,
  • Jelena Burazerović,
  • Marija Jovanović,
  • Sara Arsenijević and
  • Miloš Stupar

Cave ecosystems represent environmentally constrained habitats that host diverse and highly specialized fungal communities. Many cave-dwelling fungi act as decomposers, transient colonizers, or cave fauna symbionts. During a mycological survey of Ses...

  • Article
  • Open Access
469 Views
16 Pages

Bacterial Resistance in the ICU: A Comparative Analysis of Pre-Pandemic and Pandemic Periods and the Impact on Clinical Outcomes

  • Geane Andriollo Paradynski,
  • Ronaldo dos Santos Machado,
  • Lucas Machado Sulzbacher,
  • Maicon Machado Sulzbacher,
  • Vítor Antunes de Oliveira,
  • Pauline Brendler Goettems Fiorin,
  • Mirna Stela Ludwig,
  • Thiago Gomes Heck and
  • Matias Nunes Frizzo

Inappropriate use of antibiotics can stimulate antimicrobial resistance, since bacteria are capable of circumventing pharmacological action through various resistance mechanisms. Recently, during the COVID-19 pandemic, there has been an increase in t...

  • Article
  • Open Access
307 Views
14 Pages

Salivary Oral Microbiota in Patients with Prediabetes Undergoing Intragastric Balloon Surgery

  • Rabab A. D. Meshan,
  • Norah Ahmed AlOsaimi,
  • Abdulmohsen Redha and
  • Maribasappa Karched

Kuwait faces a significant public health challenge from obesity and Type 2 Diabetes Mellitus (T2DM), conditions known to disrupt the natural balance of oral bacteria. This imbalance, or dysbiosis, can promote gum disease and may worsen metabolic heal...

  • Article
  • Open Access
285 Views
14 Pages

Pseudomonas extremorientalis PEY1, isolated from the intestinal contents of marine fish, was evaluated for the production and properties of antibacterial proteins active against Edwardsiella tarda, a major pathogen in aquaculture. Antibacterial produ...

  • Communication
  • Open Access
427 Views
9 Pages

Growth of Ectomycorrhizal Fungi on Inorganic and Organic Nitrogen Sources

  • Burenjargal Otgonsuren,
  • Hangyu Lan and
  • Douglas L. Godbold

In forest soils, nitrogen (N) is present in inorganic and organic forms. The organic forms include monomeric amino acids, but also polymers such as chitin. Ectomycorrhizal fungi are known to take up both inorganic and organic N forms, and to depolyme...

  • Review
  • Open Access
577 Views
27 Pages

Regulatory Mechanisms of Fumonisin Biosynthesis and Applications in Food Safety and Biotechnology

  • Lei Fan,
  • Yuqing Lei,
  • Zhihui Qi,
  • Haiyang Zhang,
  • Lin Tian and
  • Fang Tang

Fumonisins, a major class of mycotoxins, pose significant health risks to humans and animals due to their widespread contamination and potent toxicity. Recent advances in molecular biology, biochemistry, and enzymology have greatly enhanced the under...

  • Article
  • Open Access
720 Views
15 Pages

Ureaplasma species are associated with various reproductive health issues, while human papillomavirus (HPV) is associated with cervical, vaginal and vulvar cancers. Data on the association between Ureaplasma species and HPV are limited in South Afric...

  • Article
  • Open Access
795 Views
13 Pages

Spatio-Temporal Presumptive Identification of Enterococcus spp. and Vibrio spp. in Water from the Veracruz Reef System National Park in the Central Gulf of Mexico

  • Fátima Jael Olvera-Muñoz,
  • Martina Hilda Gracia-Valenzuela,
  • Fabiola Lango-Reynoso,
  • Olaya Pirene Castellanos-Onorio,
  • Jesús Montoya-Mendoza,
  • Christian Reyes-Velázquez,
  • María de Lourdes Fernández-Peña,
  • Bani Mariana Ruesgas-Ramon and
  • María del Refugio Castañeda-Chávez

The Veracruz Reef System National Park (VRSNP), located in the central Gulf of Mexico, is one of the country’s most ecologically and economically significant coral systems. Despite its high biodiversity and ecosystem functionality, it is affect...

  • Article
  • Open Access
533 Views
19 Pages

Lysinibacillus as Microbial Nanofactories: Genomic Mechanisms for Green Synthesis of Silver Nanoparticles (AgNPs)

  • José Luis Aguirre-Noyola,
  • Gustavo Cuaxinque-Flores,
  • Jorge David Cadena-Zamudio,
  • Marco A. Ramírez-Mosqueda,
  • Lorena Jacqueline Gómez-Godínez and
  • Juan Ramos-Garza

The green synthesis of silver nanoparticles (AgNPs) by bacteria is a strategic route for sustainable nanobiotechnology; however, the genomic and biochemical mechanisms that make it possible remain poorly defined. In this study, bacteria native to sil...

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Microbiol. Res. - ISSN 2036-7481