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Journal Description
Horticulturae
Horticulturae
is an international, peer-reviewed, open access journal published monthly online by MDPI. The Spanish Society of Horticultural Sciences (SECH) is affiliated with Horticulturae and its members receive a discount on the article processing charges.
- Open Access— free for readers, with article processing charges (APC) paid by authors or their institutions.
- High Visibility: indexed within Scopus, SCIE (Web of Science), PubAg, AGRIS, FSTA, and other databases.
- Journal Rank: JCR - Q1 (Horticulture) / CiteScore - Q2 (Horticulture)
- Rapid Publication: manuscripts are peer-reviewed and a first decision is provided to authors approximately 16.9 days after submission; acceptance to publication is undertaken in 2.4 days (median values for papers published in this journal in the second half of 2024).
- Recognition of Reviewers: reviewers who provide timely, thorough peer-review reports receive vouchers entitling them to a discount on the APC of their next publication in any MDPI journal, in appreciation of the work done.
Impact Factor:
3.1 (2023);
5-Year Impact Factor:
3.1 (2023)
Latest Articles
Evaluation of the Effectiveness of the UNet Model with Different Backbones in the Semantic Segmentation of Tomato Leaves and Fruits
Horticulturae 2025, 11(5), 514; https://doi.org/10.3390/horticulturae11050514 - 9 May 2025
Abstract
Timely identification of crop conditions is relevant for informed decision-making in precision agriculture. The initial step in determining the conditions that crops require involves isolating the components that constitute them, including the leaves and fruits of the plants. An alternative method for conducting
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Timely identification of crop conditions is relevant for informed decision-making in precision agriculture. The initial step in determining the conditions that crops require involves isolating the components that constitute them, including the leaves and fruits of the plants. An alternative method for conducting this separation is to utilize intelligent digital image processing, wherein plant elements are labeled for subsequent analysis. The application of Deep Learning algorithms offers an alternative approach for conducting segmentation tasks on images obtained from complex environments with intricate patterns that pose challenges for separation. One such application is semantic segmentation, which involves assigning a label to each pixel in the processed image. This task is accomplished through training various models of Convolutional Neural Networks. This paper presents a comparative analysis of semantic segmentation performance using a convolutional neural network model with different backbone architectures. The task focuses on pixel-wise classification into three categories: leaves, fruits, and background, based on images of semi-hydroponic tomato crops captured in greenhouse settings. The main contribution lies in identifying the most efficient backbone-UNet combination for segmenting tomato plant leaves and fruits under uncontrolled conditions of lighting and background during image acquisition. The Convolutional Neural Network model UNet is is implemented with different backbones to use transfer learning to take advantage of the knowledge acquired by other models such as MobileNet, VanillaNet, MVanillaNet, ResNet, VGGNet trained with the ImageNet dataset, in order to segment the leaves and fruits of tomato plants. Highest percentage performance across five metrics for tomato plant fruit and leaves segmentation is the MVanillaNet-UNet and VGGNet-UNet combination with and respectively. A comparison of the best results of semantic segmentation versus those obtained with a color-dominant segmentation method optimized with a greedy algorithm is presented.
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(This article belongs to the Section Vegetable Production Systems)
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Characterization of Plant Defensin (PDF) Genes in Banana (Musa acuminata) Reveals the Antifungal Ability of MaPDF2.2 to Fusarium Wilt Pathogens
by
Ruide Li, Bin Wang, Huan Wu and Chunzhen Cheng
Horticulturae 2025, 11(5), 513; https://doi.org/10.3390/horticulturae11050513 - 9 May 2025
Abstract
Plant defensin (PDF/DEF), an important pathogenesis-related protein which widely exists in plants, displays broad-spectrum antifungal activities. To date, however, reports on the banana PDFs are very limited. In this study, we identified, cloned, and characterized the five Class I PDFs (MaPDF2.1~MaPDF2.5)
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Plant defensin (PDF/DEF), an important pathogenesis-related protein which widely exists in plants, displays broad-spectrum antifungal activities. To date, however, reports on the banana PDFs are very limited. In this study, we identified, cloned, and characterized the five Class I PDFs (MaPDF2.1~MaPDF2.5) in banana (Musa acuminata). Further, their expression in root, corm, leaf, and fruit were studied. MaPDFs exhibited quite different expression patterns in different organs, with MaPDF2.2 as the only member expressing in all the tested organs, and its expression levels in all organs were the highest among all MaPDFs. The MaPDF2.2 expression could be significantly upregulated by both low- and high-temperature stresses but significantly downregulated by the inoculations of plant growth promoting endophytic fungus Serendipita indica and banana Fusarium wilt (FW) pathogen Fusarium oxysporum f. sp. cubense (Foc) Tropical race 4 (FocTR4). Moreover, the S. indica pre-colonization could significantly alleviate the suppression of FocTR4 on MaPDF2.2, suggesting that this MaPDF might contribute greatly to the S. indica-enhanced FW resistance. By using tobacco leaf transient overexpression, the function of MaPDF2.2 was investigated. Its overexpression significantly inhibited the infection of Foc race 1 (Foc1) and FocTR4 in tobacco leaves. Furthermore, in vitro antifungal ability assays revealed that the recombinant His-MaPDF2.2 protein could significantly inhibit the growth of Foc1 and FocTR4, as well as the pigment accumulation of Foc1. Our study revealed the sequence and expression characteristics of banana PDFs and demonstrated the antifungal ability of MaPDF2.2 to FW pathogens.
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(This article belongs to the Special Issue Mitigating Soil-Borne Diseases in Horticultural Crops: Current Challenges and Management Strategies)
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Open AccessEditorial
Effects of Light Quantity and Quality on Horticultural Crops
by
László Balázs and Gergő Péter Kovács
Horticulturae 2025, 11(5), 512; https://doi.org/10.3390/horticulturae11050512 - 8 May 2025
Abstract
Light plays a fundamental role in the growth and development of plants [...]
Full article
(This article belongs to the Special Issue Effects of Light Quantity and Quality on Horticultural Crops)
Open AccessArticle
Application of Diverse Nutrients (N, P, and K) Throughout the Annual Growth Cycle Influences the Phenology and Biomass of Paeonia ostii
by
Zi-Jian Li, Shui-Yan Yu, Jia-Jie Fang, Ying Zhang, Zheng Wang, Hong-Bing Wang and Yong-Hong Hu
Horticulturae 2025, 11(5), 511; https://doi.org/10.3390/horticulturae11050511 - 8 May 2025
Abstract
The tree peony holds significant historical, cultural, and practical value. P. ostii is extensively cultivated in China, where it represents the primary oil-producing variety of tree peonies. However, the current nutrient supplementation system for P. ostii lacks an empirical basis, resulting in frequent
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The tree peony holds significant historical, cultural, and practical value. P. ostii is extensively cultivated in China, where it represents the primary oil-producing variety of tree peonies. However, the current nutrient supplementation system for P. ostii lacks an empirical basis, resulting in frequent wastage of nutrients during daily production. In this study, varying ratios and quantities of nitrogen, phosphorus, and potassium were administered to P. ostii at distinct phases of its annual growth cycle, specifically during the bud sprouting, post-flowering, and dormancy periods. The results showed that during the bud sprouting period, the plants treated with a high nitrogen and potassium ratio (a high N&K ratio, N–P–K = 35%–20%–35%) had better flowering traits than those treated with a high phosphorus ratio (a high P ratio, N–P–K = 20%–35%–20%). Under the standard application amount, plants treated with a high N&K ratio outperformed those treated with a high P ratio in terms of flowering duration, net photosynthetic rate, and flowering biomass, with increases of 20.9%, 10.7%, and 32.9%, respectively. During the post-flowering period, all plants died when treated with 1.5 × standard amounts of the above ratios. At this period, the all-high ratio with N–P–K = 45%–45%–45% resulted in a 70.4% increase in fruit set, a 43.8% increase in seed number, and a 153.8% increase in biomass compared with the high N&K ratio. During the dormancy period, nutrient supplementation with the standard amount of P led to higher increases in ground diameter and biomass. Therefore, in subsequent tree peony production, particular attention should be paid to nutrient supplementation during the post-flowering period to prevent excessive fertilizer application and safeguard the plants’ normal growth and development.
Full article
(This article belongs to the Special Issue Ornamental Plants under Abiotic Stresses)
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Open AccessArticle
Seasonality in Apple Leaf Molybdenum Contents During the Growing Season and Stages of Greatest Need in This Nutrient
by
Andrei I. Kuzin, Alexey V. Koushner, Ludmila V. Stepantsova and Andrei V. Gritsenko
Horticulturae 2025, 11(5), 510; https://doi.org/10.3390/horticulturae11050510 - 8 May 2025
Abstract
Molybdenum (Mo) is an important nutrient participating in nitrogen, sulfur, iron, and copper metabolism, as well as a cofactor in the biosynthesis of auxin and abscisic acid. Apple leaf Mo seasonality and periods of apple tree demand remain unclear due to insufficient data.
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Molybdenum (Mo) is an important nutrient participating in nitrogen, sulfur, iron, and copper metabolism, as well as a cofactor in the biosynthesis of auxin and abscisic acid. Apple leaf Mo seasonality and periods of apple tree demand remain unclear due to insufficient data. This study aimed to identify peak leaf Mo levels throughout the growing season to distinguish when apple trees require the most Mo. To analyze seasonal patterns, we determined the leaf Mo contents 11–12 times per season on untreated and Mo-treated apple trees over four seasons in 2020–2023. Foliar fertilizing stimulated a significant increase in leaf Mo status. The Mo contents in untreated tree leaves were significantly lower than in trees after foliar fertilizing. However, in fertilized trees, nutrient contents were low after ten-day periods of heavy precipitation. High leaf Mo levels coincided with periods of intense apple shoot and fruit growth (average Mo level of 0.305 mg kg−1 in untreated trees and 0.386 mg kg−1 in fertilized ones) and apple fruit development and ripening (average Mo level of 0.754 mg kg−1 in untreated tree leaves and 1.069 mg kg−1 in fertilized ones). Mo application via foliar fertilizing in July’s heat is essential for apple tree fruit growth and development to counter negative weather effects. Foliar fertilizing significantly increased tree yields in 2020, 2022, and 2023, resulting in substantially higher overall productivity (169.7 kg/tree−1 without vs. 215.6 kg/tree−1 with fertilizer).
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(This article belongs to the Section Fruit Production Systems)
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Open AccessArticle
Genome-Wide Analysis of Mlo Genes and Functional Characterization of Cm-mlo38 and Cm-mlo44 in Regulating Powdery Mildew Resistance in Melon
by
Fangyi Gong, Yanhong Lan, Tian Zhang, Chun Li, Yifan Li, Feng Xia, Xiaojun Liu, Duchen Liu, Genyun Liang, Peng Cai and Chao Fang
Horticulturae 2025, 11(5), 509; https://doi.org/10.3390/horticulturae11050509 - 8 May 2025
Abstract
The Mildew Resistance Locus (Mlo) gene family is reported in various species as regulators of powdery mildew (PM) resistance. However, the Mlo genes in cucurbit crops remain limited. In this study, a genome-wide investigation of Mlo genes was conducted in eight Cucurbitaceae species
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The Mildew Resistance Locus (Mlo) gene family is reported in various species as regulators of powdery mildew (PM) resistance. However, the Mlo genes in cucurbit crops remain limited. In this study, a genome-wide investigation of Mlo genes was conducted in eight Cucurbitaceae species and in rice, maize, arabidopsis, and barley, and a total of 202 Mlo genes were identified. The phylogenetic analysis showed that 202 Mlo genes can be classified into six clades, and the Mlo genes from clades I and III are likely pivotal in regulating PM resistance in dicotyledonous and monocotyledonous plants, respectively. The Ka/Ks ratios for these homologous Mlo gene pairs ranged from 0 to 0.6, revealing that they underwent substantial purifying selection during evolution. Among 12 crops, there were the most Mlo genes (22 Cm-mlo) in melon. An expression analysis revealed that six Cm-mlo genes showed expression responses to PM infection in which Cm-mlo38 and Cm-mlo44 were phylogenetically close to Mlo genes that regulated PM resistance. Using the VIGS system for silencing, Cm-mlo38 and Cm-mlo44 enhanced resistance to PM in susceptible material. A protein interaction analysis indicated that Cm-mlo38 might regulate PM resistance through interactions with PR5 and CML proteins. These results provide a comprehensive understanding of the Mlo family in Cucurbitaceae and pave the way for future functional analysis and genetic improvement for improving PM resistance in Cucurbitaceae.
Full article
(This article belongs to the Special Issue Genomics and Genetic Diversity in Vegetable Crops)
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Open AccessArticle
Response to Sensor-Based Fertigation of Nagpur Mandarin (Citrus reticulata Blanco) in Vertisol of Central India
by
Deodas Meshram, Anoop Kumar Srivastava, Akshay Utkhede, Chetan Pangul and Vasileios Ziogas
Horticulturae 2025, 11(5), 508; https://doi.org/10.3390/horticulturae11050508 - 8 May 2025
Abstract
In citriculture, inputs like water and fertilizer are applied through traditional basin methods, thereby incurring reduced use-efficiency. The response of conventional crop coefficient-based fertigation scheduling continues to be inconsistent and complex in its field implementation, thereby necessitating the intervention of sensor-based (Internet of
[...] Read more.
In citriculture, inputs like water and fertilizer are applied through traditional basin methods, thereby incurring reduced use-efficiency. The response of conventional crop coefficient-based fertigation scheduling continues to be inconsistent and complex in its field implementation, thereby necessitating the intervention of sensor-based (Internet of Things; IoT) technology for fertigation scheduling on a real-time basis. The study aimed to investigate fertigation scheduling involving four levels of irrigation, viz., I1 (100% evapotranspiration (ET) as the conventional practice), I2 (15% volumetric moisture content (VMC)), I3 (20% VMC), and I4 (25% VMC), as the main treatments and three levels of recommended doses of fertigation, achieved by reappropriating different nutrients across phenologically defined critical growth stages, viz., F1, F2, and F3 (conventional fertilization practice), as sub-treatments, which were evaluated through a split-plot design over two harvesting seasons in 2021–2023. Nagpur mandarin (Citrus reticulata Blanco) was used as the test crop, which was raised on Indian Vertisol facing multiple nutrient constraints. Maximum values for physiological growth parameters (plant height, canopy area, canopy volume, and relative leaf water content (RLWC)) and fruit yield (characterized by 9% and 5%, respectively, higher A-grade-sized fruits with the I4 and F1 treatments over corresponding conventional practices, viz., I1 and F3) were observed with the I4 irrigation treatment in combination with the F1 fertilizer treatment (I4F1). Likewise, fruit quality parameters, viz., juice content, TSS, TSS: acid ratio, and fruit diameter, registered significantly higher with the I4F1 treatment, featuring the application of B at the new-leaf initiation stage (NLI) and Zn across the crop development (CD), color break (CB), and crop harvesting (CH) growth stages, which resulted in a higher leaf nutrient composition. Treatment I4F1 conserved 20–30% more water and 65–87% more nutrients than the I1F3 treatment (conventional practice) by reducing the rate of evaporation loss of water, thereby elevating the plant’s available nutrient supply within the root zone. Our study suggests that I4F1 is the best combination of sensor-based (IoT) irrigation and fertilization for optimizing the quality production of Nagpur mandarin, ensuring higher water productivity (WP) and nutrient-use-efficiency (NUE) coupled with the improved nutritional quality of the fruit.
Full article
(This article belongs to the Special Issue Orchard Management: Strategies for Yield and Quality)
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Open AccessArticle
Identification and Characterization of the BBX Gene Family in Pomegranate (Punica granatum L.) and Its Potential Role in Anthocyanin Accumulation During Fruit Ripening
by
Longbo Liu and Jie Zheng
Horticulturae 2025, 11(5), 507; https://doi.org/10.3390/horticulturae11050507 - 8 May 2025
Abstract
B-box (BBX) genes, as zinc finger transcription factors (TFs), play essential roles in regulating plant growth and development. In this study, we identified 23 BBX genes in the pomegranate (Punica granatum L.) genome. These genes were classified into five groups based on
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B-box (BBX) genes, as zinc finger transcription factors (TFs), play essential roles in regulating plant growth and development. In this study, we identified 23 BBX genes in the pomegranate (Punica granatum L.) genome. These genes were classified into five groups based on the distribution of conserved domains and phylogenetic relationships. Each PgBBX group exhibited similar molecular weights, theoretical isoelectric points (pI), gene structures, and conserved motif distributions compared with BBX members in Arabidopsis and Chinese white pear in corresponding groups. Syntenic analysis revealed segmental duplications of eight PgBBX gene pairs within the pomegranate genome. Additionally, twenty-seven and thirty-one orthologous BBX pairs were identified between PgBBX and AtBBX, and PgBBX and PbBBX, respectively. Promoter analysis revealed the presence of five types of cis-acting elements responding to light, phytohormones, stress, developmental signaling, and potential transcription factors (TFs). GO enrichment analysis confirmed that most PgBBX genes function as TF involved in plant growth and development. RNA-seq data indicated that PgBBX5 was primarily expressed in leaves and flowers, with increased expression in different fruit tissues during ripening. Moreover, PgBBX5 showed a high degree of sequence similarity with anthocyanin-related homologs, including AtBBX24, PhBBX24, FaBBX24, MdCOL4, and PyBBX24. During the ripening of ‘Tunisia’ fruits, PgBBX5 expression was positively correlated with the dynamic changes in anthocyanin content and the expression of key anthocyanin biosynthetic and transport genes. Furthermore, subcellular localization suggested that PgBBX5 encodes a nuclear-localized protein. This study provides a comprehensive characterization of the PgBBX family, offering valuable insights into the mechanisms underlying anthocyanin accumulation during pomegranate fruit ripening.
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(This article belongs to the Special Issue Color Formation and Regulation in Horticultural Plants)
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Open AccessFeature PaperReview
Research and Innovations in Latin American Vitiviniculture: A Review
by
Gastón Gutiérrez-Gamboa and Mercedes Fourment
Horticulturae 2025, 11(5), 506; https://doi.org/10.3390/horticulturae11050506 - 8 May 2025
Abstract
Latin America offers a unique point of view into the adaptation of viticulture to climate change through its rich diversity of climates, traditional knowledge, and scientific innovation. This review synthesizes the current research and technological developments across major wine-producing countries including Argentina, Brazil,
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Latin America offers a unique point of view into the adaptation of viticulture to climate change through its rich diversity of climates, traditional knowledge, and scientific innovation. This review synthesizes the current research and technological developments across major wine-producing countries including Argentina, Brazil, Chile, Uruguay, the Dominican Republic, and Haiti. Argentina shows key adaptation strategies, including high-altitude vineyard relocation, clonal and rootstock selection, canopy and water management, and the conservation of Criolla and other autochthonous grapevine varieties. In Brazil, tropical viticulture and breeding programs led by Embrapa exemplify advancements in disease-resistant and climate-resilient cultivars. Chile’s heroic and southern viticulture highlights the importance of old vines, microclimatic heterogeneity, and territorial identity. Uruguay stands out for its terroir-based research and producer-led adaptation strategies. This review also addresses systemic challenges in scientific publishing, particularly the underrepresentation of Latin American researchers in global vitivinicultural discourse. These disparities underscore the need for inclusive science that values local knowledge and promotes equity in research funding and dissemination. Overall, Latin America stands out not only as a region highly vulnerable to climate change, but as an emerging model of adaptation and innovation, demonstrating how resilient, sustainable, and culturally rooted wine production can thrive under shifting environmental conditions.
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(This article belongs to the Section Viticulture)
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Open AccessArticle
Carrot (Daucus carota L.) Haploid Embryo Genome Doubling with Colchicine and Trifluralin
by
Maria Fomicheva, Elena Kozar and Elena Domblides
Horticulturae 2025, 11(5), 505; https://doi.org/10.3390/horticulturae11050505 - 8 May 2025
Abstract
The production of carrot (D. carota L.) doubled haploids (DH) for the acceleration of this important vegetable crop breeding requires genome doubling of haploid regenerants. If spontaneous doubling does not occur, artificial chromosome doubling can be complicated by the lack of efficient
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The production of carrot (D. carota L.) doubled haploids (DH) for the acceleration of this important vegetable crop breeding requires genome doubling of haploid regenerants. If spontaneous doubling does not occur, artificial chromosome doubling can be complicated by the lack of efficient genome-doubling protocols. We tested an antimitotic agent treatment of carrot at the embryo stage. It allowed us to produce and treat a large number of clonal carrot embryos (at least 30 embryos per treatment condition) in small volumes with minimal reagent amounts. We showed that 0.01–1 g/L colchicine did not perturb carrot development. Trifluralin showed no signs of toxicity at 0.001 and 0.01 g/L concentrations, but 0.1 g/L trifluralin reduced survival by 40% and delayed plantlet regeneration. We showed via DNA content flow cytometry that 0.01–1 g/L colchicine and 0.001–0.1 g/L trifluralin could double the carrot genome. The highest diploid percent was observed at 1 g/L colchicine (34%) and 0.1 g/L trifluralin (28%). The highest percent of diploids together with mixoploids (partial diploids) was at 0.01 and 0.1 g/L trifluralin (over 70%), followed by 1 g/L colchicine (56%). To our knowledge, this is the first report on trifluralin application for genome doubling in Apiaceae. In our study, we determined colchicine and trifluralin toxicity and doubling efficiency at different concentrations that can be used for carrot DH-line production and further improvement of genome doubling methods.
Full article
(This article belongs to the Special Issue A Decade of Research on Vegetable Crops: From Omics to Biotechnology)
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Open AccessArticle
Comprehensive Genome-Wide Characterization of the GRAS Gene Family and Their Role in Salt Stress Tolerance in Punica granatum L.
by
Jingyi Huang, Hongfang Ren, Fan Cheng, Yingfen Teng, Xueqing Zhao and Zhaohe Yuan
Horticulturae 2025, 11(5), 504; https://doi.org/10.3390/horticulturae11050504 - 7 May 2025
Abstract
The GRAS gene family is broadly distributed in plants and plays key regulatory roles in development, signal transduction, and the adaptation to adverse environments. Pomegranate (Punica granatum L.)—a high-value fruit tree with ecological, economic, health, and ornamental importance—exhibits notable salt tolerance. While
[...] Read more.
The GRAS gene family is broadly distributed in plants and plays key regulatory roles in development, signal transduction, and the adaptation to adverse environments. Pomegranate (Punica granatum L.)—a high-value fruit tree with ecological, economic, health, and ornamental importance—exhibits notable salt tolerance. While GRAS genes have been characterized in various species, their functional roles in pomegranate remain underexplored. In this study, 54 GRAS genes (PgGRAS) were identified in the pomegranate genome and were found to be unevenly distributed across eight chromosomes. Phylogenetic analysis grouped these genes into eight subfamilies, revealing highly similar conserved motifs, functional domains, and gene structures within each group. Notably, the DELLA subfamily is distinguished by a unique DELLA domain. Our findings indicate that the expansion of GRAS genes in pomegranate may be linked to fragment duplication events, and many PgGRAS genes contain both phytohormone- and stress-responsive cis-elements. Under 200 mM NaCl treatment, the expression of two DELLA genes was markedly upregulated. Therefore, PgGRAS24 was selected as a candidate gene for stable expression in Arabidopsis to further verify the role of DELLA family members in plant salt tolerance. Overall, this study provides new insights into the molecular functions of the GRAS gene family in pomegranate, gives insights into their role in salt stress tolerance, and lays a theoretical foundation for developing salt-tolerant pomegranate varieties.
Full article
(This article belongs to the Special Issue Advances in Developmental Biology in Tree Fruit and Nut Crops—2nd Edition)
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Open AccessArticle
Viral and Viroid Communities in Peach Cultivars Grown in Bulgaria
by
Mariyana Gozmanova, Vesselin Baev, Rumyana Valkova, Elena Apostolova-Kuzova, Stoyanka Jurac, Galina Yahubyan, Lilyana Nacheva and Snezhana Milusheva
Horticulturae 2025, 11(5), 503; https://doi.org/10.3390/horticulturae11050503 - 7 May 2025
Abstract
Peaches (Prunus persica L. Batsch) and nectarines (Prunus persica L. Batsch var. nectarina [Ait.] Maxim) are economically important stone fruits consumed worldwide, both fresh and processed. Viruses and viroids significantly constrain the cultivation and productivity of peach orchards. Climate change may
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Peaches (Prunus persica L. Batsch) and nectarines (Prunus persica L. Batsch var. nectarina [Ait.] Maxim) are economically important stone fruits consumed worldwide, both fresh and processed. Viruses and viroids significantly constrain the cultivation and productivity of peach orchards. Climate change may alter vector populations and lead to shifts in agricultural practices, influencing the spread of these viruses and viroids. Additionally, market globalization further intensifies the pressure on peach crops by facilitating the movement of pathogens, increasing the incidence of virus-induced diseases. In this study, we identified the viral and viroid communities in five peach cultivars from Bulgaria and assessed their impact on symptom development. RNA sequencing of symptomatic leaf samples revealed the presence of common peach viruses, such as plum pox virus and prunus necrotic ringspot virus. Notably, we identified peach latent mosaic viroid and cherry green ring mottle virus in Bulgarian peach orchards for the first time. Furthermore, bioassays of indicator plants, ELISA, and Sanger sequencing were performed for each peach tree to complement the RNA sequencing data. These findings provide valuable insights into the composition of viral and viroid pathogens affecting peaches in Bulgaria and will support the development of targeted strategies for monitoring and managing these pathogens, contributing to the sustainable production of peaches in the region.
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(This article belongs to the Section Biotic and Abiotic Stress)
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Open AccessArticle
A Predictive Model of the Photosynthetic Rate of Chili Peppers Using Support Vector Regression and Environmental Multi-Factor Analysis
by
Bin Li, Bo Qiao, Qianyu Zhao, Dan Yang, Rongcheng Zhu, Zhexuan Wang and Yujie Yang
Horticulturae 2025, 11(5), 502; https://doi.org/10.3390/horticulturae11050502 - 7 May 2025
Abstract
Light intensity, CO2 concentration, and temperature are three primary environmental factors with high temporal variability, nonlinearity, and strong coupling, which directly influence the photosynthetic rate of plants. To investigate the combined influence of these factors on the photosynthetic rate of chili pepper
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Light intensity, CO2 concentration, and temperature are three primary environmental factors with high temporal variability, nonlinearity, and strong coupling, which directly influence the photosynthetic rate of plants. To investigate the combined influence of these factors on the photosynthetic rate of chili pepper plants, a predictive model was developed for their net photosynthetic rate (Pn) during the peak fruiting period. A multifactorial nested experimental design with irregular sampling intervals was used to systematically examine the interacting effects of light intensity, CO2 concentration, and temperature on photosynthesis. Based on the collected data, a support vector regression (SVR) algorithm was trained and its performance was compared with that of a backpropagation (BP) neural network, a radial basis function (RBF) neural network, and a random forest (RF) algorithm. To optimize performance, a grid search with five-fold cross-validation was conducted to identify optimal hyperparameters; this process yielded a cost parameter (C) of 38 and a gamma parameter (γ) of 8, which minimized the root mean square error (RMSE) on the training set. On the test set, the SVR model achieved a coefficient of determination (R2) of 0.9941 and an RMSE of 0.6988 μmol m−2 s−1 (relative to the full Pn range of −4.19 to 39.2 μmol m−2 s−1). A linear fit between measured and predicted Pn values yielded a slope of 0.992 and an intercept of 0.07, indicating near-perfect agreement and surpassing the performance of the BP, RBF, and RF models. These results demonstrate that the SVR-based model outperformed the other approaches and exhibited superior predictive ability, establishing it as a robust theoretical foundation and a practical tool for dynamic environmental optimization in controlled-environment agriculture.
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(This article belongs to the Section Vegetable Production Systems)
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Open AccessArticle
Alginate Oligosaccharide Promoted the Nutrient Uptake and Growth of Cucumber Seedlings Under Suboptimal Temperature Conditions
by
Xu Guo, Yun Li, Kai Fan, Lingru Guo, Yongzhao Yang, Chunming Cheng, Leiping Hou, Yanxiu Miao, Meihua Sun, Yaling Li and Longqiang Bai
Horticulturae 2025, 11(5), 501; https://doi.org/10.3390/horticulturae11050501 - 7 May 2025
Abstract
Due to its sensitivity to cold temperatures, cucumber growth is substantially constrained by suboptimal temperature stress in northern China’s off-season production systems. Suboptimal temperatures severely repress the nutrient absorption, growth, and yield formation of vegetables in solar greenhouses during winter and early spring
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Due to its sensitivity to cold temperatures, cucumber growth is substantially constrained by suboptimal temperature stress in northern China’s off-season production systems. Suboptimal temperatures severely repress the nutrient absorption, growth, and yield formation of vegetables in solar greenhouses during winter and early spring in China. Alginate oligosaccharides (AOSs) are anionic acidic polysaccharides derived from brown algae, known for promoting plant growth and alleviating abiotic stress. In this study, we aimed to investigate the effects of different nutrient solution concentrations combined with AOS on the growth and nutrient uptake of cucumber seedlings under suboptimal temperatures (15/8 °C, day/night). Potted ‘Jinchun 4’ cucumber seedlings grown in coconut coir were treated with 0.5×, 1.0×, or 1.5× strength of Hoagland solution alone (N0.5, N1, N1.5), or with 30 mg·L−1 AOS (A0.5, A1, A1.5). The results showed that the growth attributes and nitrogen (N) accumulation of cucumber plants of N1 and N1.5 were significantly higher than those of N0.5. Additionally, plants of A0.5 exhibited significantly higher plant height, chlorophyll a content, root surface area, root volume, root vitality, N metabolism enzyme (NR, GDH, GS) activities, and N accumulation, than those under N0.5, N1, or N1.5. Moreover, compared to A0.5, the net photosynthetic rate, total root length, root surface area, root N content, leaf nitrate reductase activity, root glutamate dehydrogenase activity, and N accumulation of A1 and A1.5 were significantly higher than those of A0.5. Correlation analysis revealed strong linkages between root morphology traits and tissue N content. In summary, under suboptimal temperature conditions, the application of AOS improved cucumber seedlings’ nutrient absorption and growth more efficiently than merely raising nutrient levels, as it enhanced root surface area, root vitality, and N metabolic enzyme activities.
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(This article belongs to the Section Biotic and Abiotic Stress)
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Open AccessArticle
Investigation of Biomass and Carbon Storage of Tree Species in Zhengzhou, a Megacity in China
by
Jing He, Qike Ding, Qiupeng Yuan, Shanshan Jin, Dongfeng Yan and Zhi Li
Horticulturae 2025, 11(5), 500; https://doi.org/10.3390/horticulturae11050500 - 6 May 2025
Abstract
Urban trees play a dual role in enhancing landscape aesthetics and contributing to carbon sequestration. This study evaluated the biomass and carbon storage of eight common urban tree species in Zhengzhou, China, using organ-specific measurements and allometric growth models. The results revealed that
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Urban trees play a dual role in enhancing landscape aesthetics and contributing to carbon sequestration. This study evaluated the biomass and carbon storage of eight common urban tree species in Zhengzhou, China, using organ-specific measurements and allometric growth models. The results revealed that biomass and carbon were primarily allocated to the trunk, followed by the roots, branches, and leaves; these results are consistent with previous findings. Acer buergerianum Miq. and Magnolia grandiflora L. demonstrated the highest per-tree carbon storage, while Styphnolobium japonicum (L.) Schott and Salix babylonica L. performed better in carbon storage per unit area. These patterns are linked to variations in species morphology, growth performance, and planting density. The study provides evidence-based recommendations for optimizing urban tree species selection to enhance carbon sink capacity. Future applications include integrating these results into urban planning strategies and long-term carbon monitoring systems, thereby contributing to low-carbon city development.
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(This article belongs to the Special Issue Rethinking Horticulture to Meet Sustainable Development Goals)
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Open AccessFeature PaperArticle
Evaluating a Natural-Based Solution for Its Stimulation in Cucumis sativus Plants and Fruits
by
Antonios Chrysargyris, Panayiota Xylia, Menelaos Stavrinides and Nikolaos Tzortzakis
Horticulturae 2025, 11(5), 499; https://doi.org/10.3390/horticulturae11050499 (registering DOI) - 5 May 2025
Abstract
The current study researched the biostimulant impacts of a natural-based solution (NBS) that contained eucalyptus and rosemary essential oils on cucumber crops. The effects of NBS (one time-NBS1; two times-NBS2) application on plant development and physiological attributes (chlorophylls, stomatal conductance), total phenolics, non-enzymatic
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The current study researched the biostimulant impacts of a natural-based solution (NBS) that contained eucalyptus and rosemary essential oils on cucumber crops. The effects of NBS (one time-NBS1; two times-NBS2) application on plant development and physiological attributes (chlorophylls, stomatal conductance), total phenolics, non-enzymatic and enzymatic antioxidant activities, leaf minerals content, cucumber quality attributes at harvest and after one-week storage were assessed through experiments. NBS1 spraying was less effective than NBS2 application because it resulted in a decrease in mineral accumulation (like reduced nitrogen) and other physiological characteristics (like chlorophylls). The plants’ enhanced oxidative stress and activation of several enzymatic antioxidant systems were reflected in the use of a commercial solution (CS) based on amino acids and biostimulants, which also boosted stomatal conductance, reduced nitrogen, calcium, and magnesium accumulation, and antioxidant capacity. No differences were found in plant height, number of leaves, plant biomass, chlorophyll fluorescence, total phenols, and various fruit quality attributes, including firmness, fresh weight, respiration rates, total soluble solids, ascorbic acid, decay, and marketability among the treatments. In fact, the effects of both CS and NBS treatment on cucumber plants and fruits were less pronounced, suggesting that more than two applications should be explored in the future.
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(This article belongs to the Special Issue Controlled Environment Agriculture (CEA) for Vegetables, Ornamental and Aromatic Plants)
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Open AccessArticle
Analysis of the Vegetative Growth Development and Phenology of Hop Cultivars Grown in the Subtropics Under a Two-Crop-a-Year System
by
Nathalia Rodrigues Leles, Alessandro Jefferson Sato, Robson Fernando Missio, Laura Baiocco Araldi, Aline Cristina de Aguiar and Sergio Ruffo Roberto
Horticulturae 2025, 11(5), 498; https://doi.org/10.3390/horticulturae11050498 - 5 May 2025
Abstract
The aim of this study was to characterize the vegetative growth development of hop plants grown in the subtropics under a two-crop-a-year system with artificial supplementation lighting. The development of ‘Mapuche’ and ‘Spalter’ hops was compared during the summer 2022–2023, fall 2023, summer
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The aim of this study was to characterize the vegetative growth development of hop plants grown in the subtropics under a two-crop-a-year system with artificial supplementation lighting. The development of ‘Mapuche’ and ‘Spalter’ hops was compared during the summer 2022–2023, fall 2023, summer 2023–2024 and fall 2024 harvest seasons, considering the effects of the air temperature on the vegetative growth of plants from thermal sums in a subtropical climate region. The experiment was conducted in Palotina, Paraná, Brazil (24°17′40.05″ S, 55°50′23.16″ W, at 332 m elevation). The hops were trained on a 5.5 m high vertical trellis, using a ‘V’-shaped training system. Vegetative growth was evaluated based on the plant height development (m), hop growth rate (HGR), and classification of four growth stages based on the HGR. The phenology of the hop cultivars was determined visually according to the duration in days of the phenological stages. The development of the plant height and HGR was analyzed by nonlinear regressions of the Gompertz model and Gaussian function, respectively. ‘Mapuche’ and ‘Spalter’ hops had complete vegetative growth and phenological phases in the summer and fall seasons, with greater precocity in plant development in the summer season. The growth model based on the air temperature demonstrated that under subtropical conditions, the growth was maximized in seasons with higher temperatures. The duration of the phenological phases and the complete cycle of the plants was influenced by the vegetative growth of each cultivar in each harvest season. Therefore, double annual crop production of the hop cultivars ‘Mapuche’ and ‘Spalter’ is possible in a subtropical climate with artificial light supplementation.
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(This article belongs to the Topic New Trends and Innovations in Medicinal and Aromatic Plants, and Specialty Crops)
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Open AccessArticle
Ethnobotany of Lao Isan Ethnic Group from Na Chueak District, Maha Sarakham Province, Northeastern Thailand
by
Piyaporn Saensouk, Surapon Saensouk, Thawatphong Boonma, Auemporn Junsongduang, Sombat Appamaraka, Kamonwan Koompoot, Khamfa Chanthavongsa and Tammanoon Jitpromma
Horticulturae 2025, 11(5), 497; https://doi.org/10.3390/horticulturae11050497 - 4 May 2025
Abstract
This study investigates the plant diversity, ethnobotanical knowledge, and traditional uses of plants by the Lao Isan ethnic group in Na Chueak District, Maha Sarakham Province, Thailand. A total of 109 plant species, representing 48 families, were identified, with the Fabaceae, Euphorbiaceae, and
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This study investigates the plant diversity, ethnobotanical knowledge, and traditional uses of plants by the Lao Isan ethnic group in Na Chueak District, Maha Sarakham Province, Thailand. A total of 109 plant species, representing 48 families, were identified, with the Fabaceae, Euphorbiaceae, and Rubiaceae families being the most prevalent. This study highlights the ecological and cultural significance of these plants, many of which serve multiple purposes, including food, medicine, and other purposes. A use value analysis revealed that plants such as Schleichera oleosa (Lour.) Oken, Trigonostemon reidioides (Kurz) Craib, and Vietnamosasa pusilla (A.Chev. & A.Camus) T.Q.Nguyen have high functional importance in local cultural and medical practices. The Relative Frequency of Citation indicated that these species are integral to the community’s plant knowledge, with Trigonostemon reidioides and Vietnamosasa pusilla being especially prominent. Additionally, 62 species were identified for medicinal use, emphasizing the community’s reliance on plant-based remedies. This study also discusses the Informant Consensus Factor and Fidelity Level, which reveal strong agreement on the effectiveness of certain plants, particularly for treating digestive, respiratory, and wound healing conditions. This research contributes to the documentation of ethnobotanical knowledge, emphasizing the importance of traditional plant use for cultural continuity and sustainable resource management.
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(This article belongs to the Section Medicinals, Herbs, and Specialty Crops)
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Open AccessArticle
Comparative Global Metabolome Profile and Transcriptome Sequence Analysis of the Rough and Smooth Peel of the Orah Mandarin (Citrus reticulata)
by
Hongming Liu, Chunrui Long, Xiaomeng Fu, Shaohua Wang, Yuqiang Lou, Jianmei Dong, Meichao Dong, Jiamei Mao, Jiandong Yang, Hongxia Yang, Yuxia Du and Xianyan Zhou
Horticulturae 2025, 11(5), 496; https://doi.org/10.3390/horticulturae11050496 - 4 May 2025
Abstract
Background: The Orah mandarin is an economically important variety of Citrus reticulata for citrus growers in Yunnan Province, China. Generally, the fruit peel is smooth, an attractive feature for consumer preferences. Recently, rough peels have been observed in several orchards, making the fruit
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Background: The Orah mandarin is an economically important variety of Citrus reticulata for citrus growers in Yunnan Province, China. Generally, the fruit peel is smooth, an attractive feature for consumer preferences. Recently, rough peels have been observed in several orchards, making the fruit aesthetically less desirable. Little is known about the mechanism of rough skin development. Methods: In this study, we used global metabolomics and a comparative transcriptomic approach to characterize the differences between smooth (CK) and rough (CP) Orah mandarin peels. Results: Our results indicate that CP fruits have a significantly larger diameter, peel weight and thickness, total soluble solids, and titratable acid content compared to CK. Metabolomic analysis detected 810 metabolites, of which 192 were differentially accumulated in CP and CK. CP is characterized by higher levels of flavonoids, amino acids and derivatives, terpenoids, and alkaloids. We also report nine compounds detected exclusively in CP, including dambonitol, 3-methyl-L-histidine, deacetylnomilinic acid, obacunoic acid, and 6-O-acetylarbutin. The transcriptome results showed that the expression of genes enriched in flavonoids, lipid, and amino acid metabolism and related pathways were consistent with the metabolome profiles. We also discuss the possible involvement of phytohormones in peel roughening. Conclusions: Overall, we present, for the first time, a detailed comparative metabolome and transcriptome profile in smooth and rough Orah mandarin peels. Our data and discussion highlight the potential mechanisms and provide a theoretical basis for the improvement of rough peel Orah mandarins.
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(This article belongs to the Section Developmental Physiology, Biochemistry, and Molecular Biology)
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Open AccessArticle
Research on the Reproductive Strategies of Different Provenances/Families of Juglans mandshurica Maxim. Based on the Fruit Traits
by
Yitong Chen, Ruixue Guo, Xiaona Pei, Dan Peng, Zihan Yan, Mingrui Kang, Yulu Pan, Jingxin Yu, Lu Xu, Huicong Lin, Chuang Liu, Qinhui Zhang and Xiyang Zhao
Horticulturae 2025, 11(5), 495; https://doi.org/10.3390/horticulturae11050495 - 2 May 2025
Abstract
This study systematically analyzed the fruit traits of four sources and 117 families of Juglans mandshurica Maxim. in Jilin Province. By measuring key traits such as fruit phenotype and nut phenotype, the relationship between fruit characteristics and environmental adaptability was explored, leading to
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This study systematically analyzed the fruit traits of four sources and 117 families of Juglans mandshurica Maxim. in Jilin Province. By measuring key traits such as fruit phenotype and nut phenotype, the relationship between fruit characteristics and environmental adaptability was explored, leading to the selection of superior materials with high oil content potential. The study used fruit from J. mandshurica of 117 families (random sampling) across four provenances as experimental materials and measured 13 fruit phenotypic traits, including fruit length and fruit width. Finally, principal component analysis and genetic variation parameters were conducted. The results of the variance analysis (ANOVA) indicated that except for the nut roundness index, all other traits exhibited highly significant differences among provenances and families (p < 0.01). The range of genetic and phenotypic variation coefficients for the various traits was 7.47–23.23% and 8.76–29.59%. The family heritability ranged from 0.968 to 0.988. Correlation analysis among fruit traits revealed a non-significant correlation between fruit width and seed yield, fruit type index and nut weight, kernel weight and kernel yield, as well as nut longitudinal diameter and kernel yield. However, significant correlations were observed among all other traits. The Pearson correlation analysis between fruit traits and environmental factors revealed a significant negative correlation between longitude and seed yield. Cluster analysis results, based on the Euclidean distance method, showed that materials from four provenances were categorized into three groups at a genetic distance of 5. Principal Component Analysis (PCA) revealed that the cumulative contribution rate of four principal components reached 87.00%. PCI demonstrated the highest contribution rate and included traits such as fruit length, nut longitudinal diameter, nut transverse diameter, nut side diameter, three-diameter mean, and nut weight. One elite provenance and five elite families were preliminarily selected. The realized gain for the selected provenance fruit traits was higher for fruit weight and kernel weight, with values of 2.41% and 3.67%, respectively. For the selected families, the genetic gain was highest for kernel yield and kernel weight, with values of 16.51% and 26.66%, respectively. The findings will provide insights into breeding strategies to enhance walnut oil yield. The identified traits may be used to guide breeding programs for developing high-oil-content varieties; However, further validation studies are required to confirm these traits and their applicability in large-scale breeding efforts.
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(This article belongs to the Section Genetics, Genomics, Breeding, and Biotechnology (G2B2))
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