Horticulturae

15 pages, 2414 KB

Open AccessArticle

Male Date Palm Chlorotype Selection Based on Fertility, Metaxenia, and Transcription Aspects

by Hammadi Hamza, Mohamed Ali Benabderrahim, Achwak Boualleg, Federico Sebastiani, Faouzi Haouala and Mokhtar Rejili

Horticulturae 2025, 11(7), 865; https://doi.org/10.3390/horticulturae11070865 - 21 Jul 2025

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Abstract

This study evaluated the influence of different male date palm cultivars, distinguished by their chloroplast haplotypes, on pollen quality, pollination efficiency, metaxenia effects, and gene expression during fruit development. Chloroplast DNA analysis of 37 male trees revealed multiple haplotypes, from which cultivars B25, [...] Read more.

This study evaluated the influence of different male date palm cultivars, distinguished by their chloroplast haplotypes, on pollen quality, pollination efficiency, metaxenia effects, and gene expression during fruit development. Chloroplast DNA analysis of 37 male trees revealed multiple haplotypes, from which cultivars B25, P8, C22, and B46 were selected for further investigation. Pollen viability varied significantly among cultivars, with P8 and B25 exhibiting the highest germination rates and pollen tube elongation, while C22 showed the lowest. These differences correlated with pollination success: P8 and B25 achieved fertilization rates near 99%, whereas C22 remained below 43%. Pollination outcomes also varied in fruit traits. Despite its low pollen performance, C22 induced the production of larger fruits at the Bleh (Kimri) stage, potentially due to compensatory physiological mechanisms. Phytochemical profiling revealed significant cultivar effects: fruits from B25-pollinated trees had with lower moisture and polyphenol content but the higher sugar levels and soluble solids, suggesting accelerated maturation. Ripening patterns confirmed this finding, with B25 promoting the earliest ripening and B46 causing the most delayed. Gene expression analysis supported these phenotypic differences. Fruits pollinated by P8, B25, and B46 exhibited elevated levels of cell-division-related transcripts, particularly the PdCD_1 gene (PDK_XM_008786146.4, a gene encoding a cell division control protein), which was most abundant in P8. In contrast, fruits from C22-pollinated trees had the lowest expression of growth-related genes, suggesting a shift toward cell expansion rather than division. Overall, the results show the critical role of male genotype in influencing fertilization outcomes and fruit development, offering valuable insights for targeted breeding strategies at enhancing date palm productivity and fruit quality. Full article

(This article belongs to the Section Genetics, Genomics, Breeding, and Biotechnology (G2B2))

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16 pages, 16505 KB

Open AccessArticle

Delayed Starch Degradation Triggers Chromoplast Structural Aberration to Inhibit Carotenoid Cleavage: A Novel Mechanism for Flower Color Deepening in Osmanthus fragrans

by Xiangling Zeng, Yunfei Tan, Xin Wen, Qiang He, Hui Wu, Jingjing Zou, Jie Yang, Xuan Cai and Hongguo Chen

Horticulturae 2025, 11(7), 864; https://doi.org/10.3390/horticulturae11070864 - 21 Jul 2025

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Abstract

The color of flowers in Osmanthus fragrans is regulated by carotenoid metabolism. The orange-red variety, Dangui, is believed to have evolved from the yellow variety, Jingui, through a natural bud mutation. This study uses the Jingui cultivar ‘Jinqiu Gui’ (JQG) and its bud [...] Read more.

The color of flowers in Osmanthus fragrans is regulated by carotenoid metabolism. The orange-red variety, Dangui, is believed to have evolved from the yellow variety, Jingui, through a natural bud mutation. This study uses the Jingui cultivar ‘Jinqiu Gui’ (JQG) and its bud mutation cultivar ‘Huolian Jindan’ (HLJD) as materials, combining genome resequencing, ultrastructural observation, targeted metabolomics, and transcriptomic analysis to elucidate the molecular and cellular mechanisms underlying flower color variation. Phylogenetic analysis confirms that HLJD is a natural bud mutation of JQG. Ultrastructural observations reveal that during petal development, chromoplasts are transformed from proplastids. In HLJD petals, starch granules degrade more slowly and exhibit abnormal morphology, resulting in chromoplasts displaying crystalline, tubular, and fibrous composite structures, in contrast to the typical spherical plastoglobuli found in JQG. Targeted metabolomics identified 34 carotenoids, showing significant increases in the levels of ε-carotene, γ-carotene, α-carotene, and β-carotene in HLJD petals compared to JQG, with these levels continuing to accumulate throughout the flowering process, while the levels of the cleavage products α-ionone and β-ionone decrease. Transcriptomic analysis indicates that carotenoid metabolic pathway genes do not correlate directly with the phenotype; however, 49 candidate genes significantly associated with pigment accumulation were identified. Among these, the expression of genes such as glycoside hydrolases (LYG036752, etc.), sucrose synthase (LYG010191), and glucose-1-phosphate adenylyltransferase (LYG003610) are downregulated in HLJD. This study proposes for the first time the pathway of “starch degradation delay → chromoplast structural abnormalities → carotenoid cleavage inhibition” for deepening flower color, providing a new theoretical model for the metabolic regulation of carotenoids in non-photosynthetic tissues of plants. This research not only identifies key target genes (such as glycoside hydrolases) for the color breeding of O. fragrans but also establishes a theoretical foundation for the color enhancement of other ornamental plants. Full article

(This article belongs to the Special Issue Physiological and Molecular Biology of Ornamental Plants—2nd Edition)

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15 pages, 1273 KB

Open AccessArticle

Screening of Substrates and Optimization of Formulations for Exogenous Nutrient Bags of Morchella sextelata (Black Morel)

by Qi Yan, Weidong Zhang, Qi Wang, Tonghui Yang, Peng Wang, Ya Yu, Xiao Tan, Xueping Kang and Jiawei Wen

Horticulturae 2025, 11(7), 863; https://doi.org/10.3390/horticulturae11070863 - 21 Jul 2025

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Abstract

In the artificial cultivation of Morchella sextelata (Black Morel), exogenous nutrient bags (ENBs) commonly employ wheat grains as the primary substrate raw material. However, this approach is costly and runs counter to the “non-grain” development direction advocated by the edible mushroom industry. Under [...] Read more.

In the artificial cultivation of Morchella sextelata (Black Morel), exogenous nutrient bags (ENBs) commonly employ wheat grains as the primary substrate raw material. However, this approach is costly and runs counter to the “non-grain” development direction advocated by the edible mushroom industry. Under controlled field conditions, twelve self-made formulations were set up and compared with a conventional market formulation to comprehensively analyze their impacts on the agronomic traits, yield, soil physicochemical properties, and economic benefits of M. sextelata fruiting bodies. The research findings indicate that the nutrient bag formulations have a significant effect on soil available nutrients. Specifically, the contents of alkali-hydrolysable nitrogen (AN) and available potassium (AK) exhibit a significantly negative correlation with M. sextelata yield (r = −0.60, p < 0.05; r = −0.72, p < 0.01, respectively). Among all the treatment groups, the KY1 formulation (comprising 30% wheat grains, 5% rice bran, 60% corncobs, 2% rice husks, 1% lime, and 1% gypsum) achieved the highest yield of 915.13 kg per 667 m², which was 16.1% higher than that of the control group. The net economic benefit per unit area (667 m²) reached CNY 75,282.15, representing a 20.7% increase compared to the traditional wheat grains-based formulation. In conclusion, partially substituting wheat grains with rice bran in ENBs can not only reduce reliance on staple food resources but also enhance yield and economic efficiency. Due to the differences in cultivated strains and environmental conditions, the impact on morel yield is substantial; therefore, the results of this study need further validation through pilot trials. Full article

(This article belongs to the Section Medicinals, Herbs, and Specialty Crops)

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22 pages, 3522 KB

Open AccessArticle

Seasonal Variation in Volatile Profiles of Lemon Catnip (Nepeta cataria var. citriodora) Essential Oil and Hydrolate

by Milica Aćimović, Biljana Lončar, Milica Rat, Mirjana Cvetković, Jovana Stanković Jeremić, Milada Pezo and Lato Pezo

Horticulturae 2025, 11(7), 862; https://doi.org/10.3390/horticulturae11070862 - 21 Jul 2025

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Abstract

Lemon catnip (Nepeta cataria var. citriodora) is an underutilized aromatic and medicinal plant known for its high essential oil yield and distinctive lemon-like scent, and is widely used in the pharmaceutical, cosmetic, food, and biopesticide industries. Unlike typical catnip, it lacks [...] Read more.

Lemon catnip (Nepeta cataria var. citriodora) is an underutilized aromatic and medicinal plant known for its high essential oil yield and distinctive lemon-like scent, and is widely used in the pharmaceutical, cosmetic, food, and biopesticide industries. Unlike typical catnip, it lacks nepetalactones and is rich in terpene alcohols, such as nerol and geraniol, making it a promising substitute for lemon balm. Despite its diverse applications, little attention has been paid to the valorization of byproducts from essential oil distillation, such as hydrolates and their secondary recovery oils. This study aimed to thoroughly analyze the volatile compound profiles of the essential oil from Lemon catnip and the recovery oil derived from its hydrolate over three consecutive growing seasons, with particular emphasis on how temperature and precipitation influence the major volatile constituents. The essential oil was obtained via semi-industrial steam distillation, producing hydrolate as a byproduct, which was then further processed using a Likens–Nickerson apparatus to extract the recovery oil, also known as secondary oil. Both essential and recovery oils were predominantly composed of terpene alcohols, with nerol (47.5–52.3% in essential oils; 43.5–54.3% in recovery oils) and geraniol (25.2–27.9% in essential oils; 29.4–32.6% in recovery oils) as the primary components. While sesquiterpene hydrocarbons were mostly confined to the essential oil, the recovery oil was distinguished by a higher presence of monooxygenated and more hydrophilic terpenes. Over the three-year period, elevated temperatures led to increased levels of geraniol, geranial, neral, and citronellal in both oils, whereas cooler conditions favored the accumulation of nerol and linalool, especially in the recovery oils. Higher precipitation was associated with elevated concentrations of nerol and linalool but decreased levels of geraniol, geranial, and neral, possibly due to dilution or degradation processes. Full article

(This article belongs to the Section Medicinals, Herbs, and Specialty Crops)

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18 pages, 3744 KB

Open AccessArticle

Effect of Plant Growth Regulators on the Physiological Response and Yield of Cucumis melo var. inodorus Under Different Salinity Levels in a Controlled Environment

by Dayane Mércia Ribeiro Silva, Francisca Zildélia da Silva, Isabelly Cristina da Silva Marques, Eduardo Santana Aires, Francisco Gilvan Borges Ferreira Freitas Júnior, Fernanda Nery Vargens, Vinicius Alexandre Ávila dos Santos, João Domingos Rodrigues and Elizabeth Orika Ono

Horticulturae 2025, 11(7), 861; https://doi.org/10.3390/horticulturae11070861 - 21 Jul 2025

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Abstract

The objective of this study was to evaluate the physiological, biochemical, and productive effects of the foliar application of bioregulators, based on auxin, cytokinin, and gibberellic acid, on yellow melon, cultivar DALI^®, plants subjected to different salinity levels in a protected [...] Read more.

The objective of this study was to evaluate the physiological, biochemical, and productive effects of the foliar application of bioregulators, based on auxin, cytokinin, and gibberellic acid, on yellow melon, cultivar DALI^®, plants subjected to different salinity levels in a protected environment to simulate Brazil’s semi-arid conditions. The experiment was conducted using a completely randomized block design, in a 4 × 3 factorial scheme, with four salinity levels (0, 2, 4, and 6 dS m⁻¹) and three doses of the bioregulator, Stimulate^® (0%, 100%, and 150% of the recommended dose), with six weekly applications. The physiological variables (chlorophyll a fluorescence and gas exchange) and biochemical parameters (antioxidant enzyme activity and lipid peroxidation) were evaluated at 28 and 42 days after transplanting, and the agronomic traits (fresh fruit mass, physical attributes, and post-harvest quality) were evaluated at the end of the experiment. The results indicated that salinity impaired the physiological and productive performance of the plants, especially at higher levels (4 and 6 dS m⁻¹), causing oxidative stress, reduced photosynthesis, and decreased yield. However, the application of the bioregulator at the 100% dose mitigated the effects of salt stress under moderate salinity (2 dS m⁻¹), promoting higher CO₂ assimilation rates of up to 31.5%, better water-use efficiency, and reduced lipid peroxidation. In addition, the fruits showed a greater mass of up to 66%, thicker pulp, and higher soluble solids (> 10 °Brix) content, making them suitable for sale in the market. The 150% dose did not provide additional benefits and, in some cases, resulted in inhibitory effects. It is concluded that the application of Stimulate^® at the recommended dose is effective in mitigating the effects of moderate salinity, up to ~3 dS m⁻¹, in yellow melon crops; however, its effectiveness is limited under high salinity conditions, requiring the use of complementary strategies. Full article

(This article belongs to the Section Protected Culture)

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16 pages, 6123 KB

Open AccessArticle

Functional Analysis of Penicillium expansum Glucose Oxidase-Encoding Gene, GOX2, and Its Expression Responses to Multiple Environmental Factors

by Yongcheng Yuan, Yutong Ru, Xiaohe Yuan, Shuqi Huang, Dan Yuan, Maorun Fu and Wenxiao Jiao

Horticulturae 2025, 11(7), 860; https://doi.org/10.3390/horticulturae11070860 - 21 Jul 2025

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Abstract

Penicillium expansum is an acidogenic fungal species that belongs to the phylum Ascomycota. During the infection and colonization of host fruits, P. expansum can efficiently express glucose oxidase (GOX) and oxidize β-D-glucose to generate gluconic acid (GLA). In this study, the bioinformatics analysis [...] Read more.

Penicillium expansum is an acidogenic fungal species that belongs to the phylum Ascomycota. During the infection and colonization of host fruits, P. expansum can efficiently express glucose oxidase (GOX) and oxidize β-D-glucose to generate gluconic acid (GLA). In this study, the bioinformatics analysis method was employed to predict and analyze the function of the GOX protein. In addition, a comprehensive assessment was conducted on the P. expansum GOX coding gene GOX2, and the expression response rules of GOX2 under different external stress environments were explored. The results show that GOX is an unstable hydrophilic protein. It is either an integrated membrane protein (such as a receptor or channel) that is directly anchored to the membrane through a transmembrane structure or a non-classical secreted protein that is secreted extracellularly. RNA-seq data analysis shows that the GOX2 gene is regulated by multiple environmental factors, including pH, temperature, carbon base, and chemical fungicides. The expression level of GOX2 reaches its maximum value under alkaline conditions (pH 8–10) and at approximately 10 °C. Using starch as the carbon source and adding sodium propionate or potassium sorbate has the effect of inhibiting the expression of the GOX2 gene. The analysis of the function of the GOX protein and the characteristics of the GOX2 gene in P. expansum provides new insights into the glucose oxidase-encoding gene GOX2. The research results provide significant value for the subsequent development of new disease resistance strategies by targeting the GOX2 gene and reducing post-harvest disease losses in fruits. Full article

(This article belongs to the Special Issue Advances in Postharvest Preservation and Quality of Fruits and Vegetables)

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21 pages, 5490 KB

Open AccessArticle

Impact of Reduced Chemical Fertilizer and Organic Amendments on Yield, Nitrogen Use Efficiency, and Soil Microbial Dynamics in Chinese Flowering Cabbage

by Jiaxin Xu, Jianshe Li, Xia Zhao, Zhen Liu, Hao Xu, Kai Cao and Lin Ye

Horticulturae 2025, 11(7), 859; https://doi.org/10.3390/horticulturae11070859 - 21 Jul 2025

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Abstract

(1) Background: The escalating issue of soil degradation caused by excessive chemical fertilizer application poses significant threats to the sustainable development of Chinese flowering cabbage (Brassica campestris L. ssp. chinensis (L.) var. utilis Tsen et Lee) production. This research aimed to identify [...] Read more.

(1) Background: The escalating issue of soil degradation caused by excessive chemical fertilizer application poses significant threats to the sustainable development of Chinese flowering cabbage (Brassica campestris L. ssp. chinensis (L.) var. utilis Tsen et Lee) production. This research aimed to identify the impacts of reduced chemical fertilizer application integrated with organic amendments on cabbage yield and rhizosphere soil microenvironment characteristics. (2) Methods: A biennial field experiment was conducted during the 2022–2023 growing seasons at Lijun Town, Yinchuan City, Ningxia Hui Autonomous Region. Five treatments were tested: (i) Control (CK, no fertilizer); (ii) Conventional chemical fertilization (CF1, chemical fertilizer only); (iii) Reduced chemical fertilization (CF2, 30% less chemical fertilizer); (iv) CF2 + Well-decomposed chicken manure (FCM, 30% less chemical fertilizer + rotted chicken manure); and (v) CF2 + Vermicompost (FEM, 30% less chemical fertilizer + vermicompost). (3) Results: In 2023, the FCM treatment reduced electrical conductivity (EC) by 24.80% and pH by 2.16%, while the FEM treatment decreased EC by 31.13% and pH by 3.84% compared to controls. The FEM treatment significantly enhanced total nitrogen content by 12.71% and 8.85% relative to CF1 and FCM treatments, respectively. Compared to CF1, FEM increased soil organic matter content by 10.49% in 2022 and 11.24% in 2023. Organic fertilizer amendments elevated available nitrogen, phosphorus, and potassium levels while enhancing sucrase activity: FCM and FEM treatments increased sucrase activity by 23.62% and 32.00%, respectively, in 2022. Organic fertilization improved bacterial diversity and richness, optimized microbial community structure, and increased the relative abundance of Bacillus. It also upregulated microbial metabolic pathways related to carbohydrate and amino acid metabolism. Soil nutrients and bacterial community structure showed positive correlations with yield, whereas soil enzyme activities exhibited negative correlations. Key factors influencing yield were identified as Proteobacteria, Chloroflexi, available potassium, organic matter, available nitrogen, Actinobacteria, Firmicutes, total nitrogen, pH, and sucrase activity. (4) Conclusions: Integrated analysis of yield and soil microenvironmental parameters demonstrates that the fertilization regimen combining 30% chemical fertilizer reduction with vermicompost amendment (FEM) constitutes a more efficient fertilization strategy for Chinese flowering cabbage, making it suitable for regional promotion in the Ningxia area. Full article

(This article belongs to the Section Plant Nutrition)

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24 pages, 7474 KB

Open AccessArticle

YOLO11m-SCFPose: An Improved Detection Framework for Keypoint Extraction in Cucumber Fruit Phenotyping

by Huijiao Yu, Xuehui Zhang, Jun Yan and Xianyong Meng

Horticulturae 2025, 11(7), 858; https://doi.org/10.3390/horticulturae11070858 - 20 Jul 2025

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Abstract

To address the issues of low efficiency and large errors in traditional manual cucumber fruit phenotyping methods, this paper proposes the application of keypoint detection technology for cucumber phenotyping and designs an improved lightweight model called YOLO11m-SCFPose. Based on YOLO11m-pose, the original backbone [...] Read more.

To address the issues of low efficiency and large errors in traditional manual cucumber fruit phenotyping methods, this paper proposes the application of keypoint detection technology for cucumber phenotyping and designs an improved lightweight model called YOLO11m-SCFPose. Based on YOLO11m-pose, the original backbone network is replaced with the lightweight StarNet-S1 backbone, reducing model complexity. Additionally, an improved C3K2_PartialConv neck module is used to enhance information interaction and fusion among multi-scale features while maintaining computational efficiency. The Focaler-IoU loss function is employed to improve keypoint localization accuracy. Results show that the improved model achieves an mAP50-95 of 0.924, with a floating-point operation count (GFLOPs) of 32.1, and reduces the model size to 1.229 × 10⁷ parameters. This model demonstrates better computational efficiency and lower resource consumption, providing an effective lightweight solution for crop phenotypic analysis. Full article

(This article belongs to the Section Vegetable Production Systems)

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17 pages, 4532 KB

Open AccessArticle

Nitric Oxide Modulates Postharvest Physiology to Maintain Abelmoschus esculentus Quality Under Cold Storage

by Xianjun Chen, Fenghuang Mo, Ying Long, Xiaofeng Liu, Yao Jiang, Jianwei Zhang, Cheng Zhong, Qin Yang and Huiying Liu

Horticulturae 2025, 11(7), 857; https://doi.org/10.3390/horticulturae11070857 - 20 Jul 2025

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Abstract

Cold storage is widely used for the postharvest preservation of fruits and vegetables; however, okra, as a tropical vegetable, is susceptible to chilling injury under low-temperature storage conditions, leading to quality deterioration, reduced nutritional value, and significant economic losses. Nitric oxide (NO), as [...] Read more.

Cold storage is widely used for the postharvest preservation of fruits and vegetables; however, okra, as a tropical vegetable, is susceptible to chilling injury under low-temperature storage conditions, leading to quality deterioration, reduced nutritional value, and significant economic losses. Nitric oxide (NO), as an important signaling molecule, plays a crucial role in the postharvest preservation of fruits and vegetables. To investigate the effects of different concentrations of nitric oxide on the postharvest quality of okra under cold storage, fresh okra pods were treated with sodium nitroprusside (SNP), a commonly used NO donor, at concentrations of 0 (control), 0.5 (T1), 1.0 (T2), 1.5 (T3), and 2.0 mmol·L⁻¹ (T4). The results showed that low-concentration NO treatment (T1) significantly reduced weight loss, improved texture attributes including hardness, springiness, chewiness, resilience, and cohesiveness, and suppressed the increase in adhesiveness. T1 treatment also effectively inhibited excessive accumulation of cellulose and lignin, thereby maintaining tissue palatability and structural integrity. Additionally, T1 significantly delayed chlorophyll degradation, preserved higher levels of soluble sugars and proteins, and enhanced the activities of key antioxidant enzymes, including superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT), contributing to improved oxidative stress resistance and membrane stability. In contrast, high-concentration NO treatments (T3 and T4) led to pronounced quality deterioration, characterized by accelerated membrane lipid peroxidation as evidenced by increased malondialdehyde (MDA) content and relative conductivity, and impaired antioxidant defense, resulting in rapid texture degradation, chlorophyll loss, nutrient depletion, and oxidative damage. These findings provide theoretical insights and practical guidance for the precise application of NO in extending shelf life and maintaining the postharvest quality of okra fruits. Full article

(This article belongs to the Section Postharvest Biology, Quality, Safety, and Technology)

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14 pages, 1393 KB

Open AccessArticle

Mitigating Water Stress and Enhancing Aesthetic Quality in Off-Season Potted Curcuma cv. ‘Jasmine Pink’ via Potassium Silicate Under Deficit Irrigation

by Vannak Sour, Anoma Dongsansuk, Supat Isarangkool Na Ayutthaya, Soraya Ruamrungsri and Panupon Hongpakdee

Horticulturae 2025, 11(7), 856; https://doi.org/10.3390/horticulturae11070856 - 20 Jul 2025

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Abstract

Curcuma spp. is a popular ornamental crop valued for its vibrant appearance and suitability for both regular and off-season production. As global emphasis on freshwater conservation increases and with a demand for compact potted plants, reducing water use while maintaining high aesthetic quality [...] Read more.

Curcuma spp. is a popular ornamental crop valued for its vibrant appearance and suitability for both regular and off-season production. As global emphasis on freshwater conservation increases and with a demand for compact potted plants, reducing water use while maintaining high aesthetic quality presents a key challenge for horticulturists. Potassium silicate (PS) has been proposed as a foliar spray to alleviate plant water stress. This study aimed to evaluate the effects of PS on growth, ornamental traits, and photosynthetic parameters of off-season potted Curcuma cv. ‘Jasmine Pink’ under deficit irrigation (DI). Plants were subjected to three treatments in a completely randomized design: 100% crop evapotranspiration (ETc), 50% ETc, and 50% ETc with 1000 ppm PS (weekly sprayed on leaves for 11 weeks). Both DI treatments (50% ETc and 50% ETc + PS) reduced plant height by 7.39% and 9.17%, leaf number by 16.99% and 7.03%, and total biomass by 21.13% and 20.58%, respectively, compared to 100% ETc. Notably, under DI, PS-treated plants maintained several parameters equivalent to the 100% ETc treatment, including flower bud emergence, blooming period, green bract number, effective quantum yield of PSII (ΔF/Fm′), and electron transport rate (ETR). In addition, PS application increased leaf area by 8.11% and compactness index by 9.80% relative to untreated plants. Photosynthetic rate, ΔF/Fm′, and ETR increased by 31.52%, 13.63%, and 9.93%, while non-photochemical quenching decreased by 16.51% under water-limited conditions. These findings demonstrate that integrating deficit irrigation with PS foliar application can enhance water use efficiency and maintain ornamental quality in off-season potted Curcuma, promoting sustainable water management in horticulture. Full article

(This article belongs to the Topic Optimizing Plants and Cultivation System for Controlled Environment Agriculture (CEA))

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19 pages, 7751 KB

Open AccessArticle

Characterization of the Salt Overly Sensitive 1 (SOS1) Pathway Genes in Tea Plant (Cameliia sinensis) Under Environmental Stress

by Shunkai Hu, Peishuo Jiang and Qirong Guo

Horticulturae 2025, 11(7), 855; https://doi.org/10.3390/horticulturae11070855 - 20 Jul 2025

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Abstract

Soil salinization poses a significant threat to tea plant (Camellia sinensis) production by compromising its bioactive compounds, such as polyphenols, L-theanine, and caffeine, which are key contributors to the plant’s health benefits and economic value. This study investigates the Salt Overly [...] Read more.

Soil salinization poses a significant threat to tea plant (Camellia sinensis) production by compromising its bioactive compounds, such as polyphenols, L-theanine, and caffeine, which are key contributors to the plant’s health benefits and economic value. This study investigates the Salt Overly Sensitive 1 (SOS1) gene family, a critical salt-tolerance regulator in tea plants, to elucidate its role in maintaining quality under environmental stress. Genome-wide analysis identified 51 CsSOS1 genes, with phylogenetic and synteny analyses revealing strong evolutionary conservation with Populus trichocarpa and Arabidopsis thaliana. Promoter analysis detected stress- and hormone-responsive cis-elements, indicating adaptive functions in abiotic stress. Expression profiling demonstrated tissue-specific patterns, highlighting significant upregulation of CsSOS1-15 and CsSOS1-41 under salt and drought stress. Co-expression network analysis further linked CsSOS1 genes to carbohydrate metabolism, implicating their roles in stress resilience and secondary metabolite synthesis. Our findings provide molecular insights into CsSOS1-mediated salt tolerance, proposing potential targets for preserving bioactive compounds. This work facilitates developing salt-resistant tea plant cultivars to ensure sustainable production and quality stability amid environmental challenges. Full article

(This article belongs to the Section Biotic and Abiotic Stress)

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16 pages, 1177 KB

Open AccessArticle

Genetic Differentiation of Ornamental and Fruit-Bearing Prunus laurocerasus Revealed by SSR and S-Locus Markers

by Attila Hegedűs, Péter Honfi, Sezai Ercisli, Gulce Ilhan, Endre György Tóth and Júlia Halász

Horticulturae 2025, 11(7), 854; https://doi.org/10.3390/horticulturae11070854 - 19 Jul 2025

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Abstract

Cherry laurel (Prunus laurocerasus) is an understudied, highly polyploid (22×) species that is widely used as an ornamental shrub and as a fruit-bearing plant in Türkiye. We analyzed 43 accessions—33 ornamental cultivars and 10 fruit-bearing selections—by examining size variations in 10 [...] Read more.

Cherry laurel (Prunus laurocerasus) is an understudied, highly polyploid (22×) species that is widely used as an ornamental shrub and as a fruit-bearing plant in Türkiye. We analyzed 43 accessions—33 ornamental cultivars and 10 fruit-bearing selections—by examining size variations in 10 simple sequence repeat (SSR) markers and the first intron region of the self-incompatibility ribonuclease (S-RNase) gene. A total of 498 alleles were detected across 11 loci, with the highest number of alleles observed at the S-locus. The SSR loci amplified between 4 (ASSR63) and 17 (BPPCT039) alleles per accession, with eight of the 11 primers generating more than 12 alleles per accession. Two markers, BPPCT040 and CPSCT021, uniquely distinguished all tested accessions. Of the alleles, only 178 (36%) were shared between the ornamental and fruit-bearing groups, reflecting significant genetic differentiation. A dendrogram and principal coordinate analysis revealed three distinct groups. Group 1 included most Hungarian and some European cultivars. Groups 2 (Western European cultivars) and 3 (Turkish selections) exhibited higher average allele numbers, suggesting greater genetic diversity in these groups. Our results indicate that cultivated cherry laurels originate from a broad genetic base and show clear genetic divergence between ornamental and fruit-bearing selections, likely due to differing long-term selection pressures. The observed genetic variability is consistent with the polyploid nature of the species and supports the presumed self-incompatible phenotype. This is the first study to report SSR fingerprints for ornamental cultivars and fruit-bearing selections, providing a potential tool for use in breeding programs. Full article

(This article belongs to the Special Issue Breeding of Ornamental Plants—Genetic Resources, New Challenges and Prospects: 2nd Edition)

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12 pages, 216 KB

Open AccessArticle

Amino Acid Biostimulants Enhance Drought and Heat Stress Tolerance of Creeping Bentgrass (Agrostis Stolonifera L.)

by Xunzhong Zhang, Mike Goatley, Maude Focke, Graham Sherman, Berit Smith, Taylor Motsinger, Catherine Roué and Jay Goos

Horticulturae 2025, 11(7), 853; https://doi.org/10.3390/horticulturae11070853 - 19 Jul 2025

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Abstract

Creeping bentgrass (Agrostis stolonifera L.) is an important cool-season turfgrass species widely used for golf course putting greens; however, it experiences a summer stress-induced quality decline in the U.S. transition zone and other regions with similar climates. The objective of this study [...] Read more.

Creeping bentgrass (Agrostis stolonifera L.) is an important cool-season turfgrass species widely used for golf course putting greens; however, it experiences a summer stress-induced quality decline in the U.S. transition zone and other regions with similar climates. The objective of this study was to determine the effects of five amino acid biostimulants on creeping bentgrass drought and heat stress tolerance. The five biostimulants, including Superbia, Amino Pro V, Siapton, Benvireo, and Surety, at the rate of 0.22 g of N m⁻², were applied biweekly to foliage, and the treatments were arranged in a randomized block design with four replications and were subjected to 56 days of heat and drought stress in growth chamber conditions. The amino acid biostimulants Superbia and Amino Pro V improved the turf quality, photochemical efficiency (PE), normalized difference vegetation index (NDVI), chlorophyll content, antioxidant enzyme superoxide dismutase activity, root growth, and viability and suppressed leaf H₂O₂ levels when compared to a control. Among the treatments, Superbia and Amino Pro V exhibited greater beneficial effects on turf quality and physiological fitness. The results of this study suggest that foliar application of amino acid biostimulants may improve the summer stress tolerance of cool-season turfgrass species in the U.S. transition zone and other regions with similar climates. Full article

(This article belongs to the Topic Biostimulants in Agriculture—2nd Edition)

18 pages, 3989 KB

Open AccessArticle

Morphological Analysis, Bud Differentiation, and Regulation of “Bud Jumping” Phenomenon in Oncidium Using Plant Growth Regulators

by Hanqiao Lan, Le Liu, Weishi Li, Daicheng Hao, Shanzhi Lin, Beilei Ye, Minqiang Tang and Peng Ling

Horticulturae 2025, 11(7), 852; https://doi.org/10.3390/horticulturae11070852 - 18 Jul 2025

Viewed by 473

Abstract

Oncidium has an important market value, with important high-grade cut orchids and potted flowers on the flower market. In the Oncidium cut flowers production industry, there is a common phenomenon that the development of vegetative buds disrupts the normal generation cycle of the [...] Read more.

Oncidium has an important market value, with important high-grade cut orchids and potted flowers on the flower market. In the Oncidium cut flowers production industry, there is a common phenomenon that the development of vegetative buds disrupts the normal generation cycle of the inflorescence induction, so-called “bud jumping”. In this study, vegetative bud differentiation and flower bud differentiation were divided into three stages, namely, the initial stage of differentiation, the leaf primordial/flower primordial differentiation stage, and the late stage of leaf bud/flower bud differentiation, as observed by paraffin sectioning. Secondly, we analyzed the differences between the vegetative buds of “bud jumping” plants and the flower buds of normal flowering plants by transcriptome sequencing. The transcriptome analysis results revealed significant differences among plant signaling pathways, particularly in gibberellins, auxins, and cytokinins, which play important roles in this phenomenon’s formation. In conjunction with the transcriptome analysis, the researchers conducted field experiments by applying plant growth regulators on the newborn pseudobulb of young Oncidium plants measuring approximately 49 mm in length. The results showed that the treatment groups of 100 mg/L of gibberellic acid (GA₃) and 100 mg/L GA₃ + 10 mg/L 6-Benziladenine (6-BA) exhibited the highest rate of flower bud differentiation instead of the least “bud jumping” phenomenon, and the “bud jumping” phenomenon was significantly reduced under 25 mg/L, 50 mg/L, and 75 mg/L 3-indoleacetic acid (IAA) treatments. The application of exogenous gibberellins, cytokinins, and auxins can effectively reduce the occurrence of “bud jumping”. Full article

(This article belongs to the Section Developmental Physiology, Biochemistry, and Molecular Biology)

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20 pages, 2144 KB

Open AccessArticle

Effects of Crop Load Management on Berry and Wine Composition of Marselan Grapes

by Jianrong Kai, Jing Zhang, Caiyan Wang, Fang Wang, Xiangyu Sun, Tingting Ma, Qian Ge and Zehua Xu

Horticulturae 2025, 11(7), 851; https://doi.org/10.3390/horticulturae11070851 - 18 Jul 2025

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Abstract

The aim of this study was to investigate the effects of the crop load on the berry and wine composition of Marselan grapes. Thus, the appropriate crop load for Marselan wine grapes in Ningxia was determined based on the shoot density and the [...] Read more.

The aim of this study was to investigate the effects of the crop load on the berry and wine composition of Marselan grapes. Thus, the appropriate crop load for Marselan wine grapes in Ningxia was determined based on the shoot density and the number of clusters per shoot. Marselan grapes from the Gezi Mountain vineyard, located at the eastern foot of Helan Mountain in the Qingtongxia region of Ningxia, were selected as the research material to conduct a combination experiment with four levels of shoot density and three levels of cluster density. The analysis of the berry and wine chemical composition was combined with a wine sensory evaluation to determine the optimal crop load levels. Crop load regulation significantly affected both the grape berry composition and the basic physicochemical properties of the resulting wine. Low crop loads improved metrics such as the berry weight and soluble solids content. A low shoot density facilitated the accumulation of organic acids, flavonols, and hydroxybenzoic acids in wine. Moderate crop loads were conducive to anthocyanin synthesis—the total individual anthocyanins content in the 10–20 shoots per meter of the canopy treatment group ranged from 116% to 490% of the control group—whereas excessive crop loads hindered its accumulation. Crop load management significantly influenced the aroma composition of wine by regulating the content of sugars, nitrogen sources, and organic acids in grape berries, thereby promoting the synthesis of esters and the accumulation of key aromatic compounds, such as terpenes. This process optimized pleasant flavors, including fruity and floral aromas. In contrast, wines from the high crop load and control treatments contained lower levels of these aroma compounds. Compounds such as ethyl caprylate and β-damascenone were identified as potential quality markers. Overall, the wine produced from vines with a crop load of 30 clusters (15 shoots per meter of canopy, 2 clusters per shoot) received the highest sensory scores. Appropriate crop load management is therefore critical to improving the chemical composition of Marselan wine. Full article

(This article belongs to the Section Viticulture)

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14 pages, 1482 KB

Open AccessArticle

The Physiological Mechanism of Arbuscular Mycorrhizal in Regulating the Growth of Trifoliate Orange (Poncirus trifoliata L. Raf.) Under Low-Temperature Stress

by Changlin Li, Xian Pei, Qiaofeng Yang, Fuyuan Su, Chuanwu Yao, Hua Zhang, Zaihu Pang, Zhonghua Yao, Dejian Zhang and Yan Wang

Horticulturae 2025, 11(7), 850; https://doi.org/10.3390/horticulturae11070850 - 18 Jul 2025

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Abstract

In recent years, low temperature has seriously threatened the citrus industry. Arbuscular mycorrhizal fungi (AMF) can enhance the absorption of nutrients and water and tolerance to abiotic stresses. In this study, pot experiments were conducted to study the effects of low-temperature stress on [...] Read more.

In recent years, low temperature has seriously threatened the citrus industry. Arbuscular mycorrhizal fungi (AMF) can enhance the absorption of nutrients and water and tolerance to abiotic stresses. In this study, pot experiments were conducted to study the effects of low-temperature stress on citrus (trifoliate orange, Poncirus trifoliata L. Raf.) with AMF (Diversispora epigaea D.e). The results showed that AMF inoculation significantly increased plant growth, chlorophyll fluorescence, and photosynthetic parameters. Compared with 25 °C, −5 °C significantly increased the relative conductance rate and the contents of malondialdehyde, hydrogen peroxide, soluble sugar soluble protein, and proline, and also enhanced the activities of catalase and superoxide dismutase, but dramatically reduced photosynthetic parameters. Compared with the non-AMF group, AMF significantly increased the maximum light quantum efficiency and steady-state light quantum efficiency at 25 °C (by 16.67% and 61.54%), and increased the same parameters by 71.43% and 140% at −5 °C. AMF also significantly increased the leaf net photosynthetic rate and transpiration rate at 25 °C (by 54.76% and 29.23%), and increased the same parameters by 72.97% and 26.67% at −5 °C. Compared with the non-AMF treatment, the AMF treatment significantly reduced malondialdehyde and hydrogen peroxide content at 25 °C (by 46.55% and 41.29%), and reduced them by 28.21% and 29.29% at −5 °C. In addition, AMF significantly increased the contents of soluble sugar, soluble protein, and proline at 25 °C (by 15.22%, 34.38%, and 11.38%), but these increased by only 9.64%, 0.47%, and 6.09% at −5 °C. Furthermore, AMF increased the activities of superoxide dismutase and catalase at 25 °C (by 13.33% and 13.72%), but these increased by only 5.51% and 13.46% at −5 °C. In conclusion, AMF can promote the growth of the aboveground and underground parts of trifoliate orange seedlings and enhance their resistance to low temperature via photosynthesis, osmoregulatory substances, and their antioxidant system. Full article

(This article belongs to the Special Issue Horticultural Crops against Abiotic Stresses: Adaptation Skills and Agronomic Strategies)

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25 pages, 657 KB

Open AccessFeature PaperArticle

Deficit Irrigation and Nitrogen Application Rate Influence Growth and Yield of Four Potato Cultivars (Solanum tuberosum L.)

by Abdulssamad M. H. Barka, Samuel Y. C. Essah and Jessica G. Davis

Horticulturae 2025, 11(7), 849; https://doi.org/10.3390/horticulturae11070849 - 18 Jul 2025

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Abstract

Potatoes have high nitrogen (N) and irrigation requirements. Increasing water scarcity and environmental concerns highlight the need for efficient resource management. This study evaluated the effects of deficit irrigation and reduced N on yield and growth parameters in four potato cultivars (Canela Russet, [...] Read more.

Potatoes have high nitrogen (N) and irrigation requirements. Increasing water scarcity and environmental concerns highlight the need for efficient resource management. This study evaluated the effects of deficit irrigation and reduced N on yield and growth parameters in four potato cultivars (Canela Russet, Mesa Russet, Russet Norkotah3, and Yukon Gold) at Colorado State University’s San Luis Valley Research Center over two growing seasons. Three irrigation levels (~70%, ~80%, and 100% ET replacement) and two N rates (165 and 131 kg/ha) were evaluated. Measurements included total and marketable yield, tuber size distribution, tuber bulking (TB), leaf area index (LAI), and stem and tuber numbers. Yield losses were absent with ≤18% irrigation reduction in Canela Russet, Mesa Russet, or Yukon Gold but occurred with larger deficits. Russet Norkotah3 experienced yield decline with 16–23% reductions in irrigation. A twenty percent reduction in N application had no effect on Mesa Russet or Russet Norkotah3 yields, while the other varieties experienced a yield decline in one out of two years. Early-season LAI and late-season TB were positively correlated with yield, particularly for Canela Russet and Russet Norkotah3. These findings suggest irrigation and N inputs can be reduced without compromising productivity, but reductions must be determined on a cultivar-by-cultivar basis. Full article

(This article belongs to the Special Issue Advances in Sustainable Cultivation of Horticultural Crops)

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19 pages, 4603 KB

Open AccessArticle

Genome-Wide Identification and Analysis of the CCT Gene Family Contributing to Photoperiodic Flowering in Chinese Cabbage (Brassica rapa L. ssp. pekinensis)

by Wei Fu, Xinyu Jia, Shanyu Li, Yang Zhou, Xinjie Zhang, Lisi Jiang and Lin Hao

Horticulturae 2025, 11(7), 848; https://doi.org/10.3390/horticulturae11070848 - 17 Jul 2025

Viewed by 518

Abstract

Photoperiod sensitivity significantly affects the reproductive process of plants. The CONSTANS, CONSTANS-LIKE, and TOC1 (CCT) genes play pivotal roles in photoperiod sensitivity and regulating flowering time. However, the function of the CCT gene in regulating flowering varies among different species. [...] Read more.

Photoperiod sensitivity significantly affects the reproductive process of plants. The CONSTANS, CONSTANS-LIKE, and TOC1 (CCT) genes play pivotal roles in photoperiod sensitivity and regulating flowering time. However, the function of the CCT gene in regulating flowering varies among different species. Further research is needed to determine whether it promotes or delays flowering under long-day (LD) or short-day (SD) conditions. CCT MOTIF FAMILY (CMF) belongs to one of the three subfamilies of the CCT gene and has been proven to be involved in the regulation of circadian rhythms and flowering time in cereal crops. In this study, 60 CCT genes in Chinese cabbage were genome-wide identified, and chromosomal localization, gene duplication events, gene structure, conserved domains, co-expression networks, and phylogenetic tree were analyzed by bioinformatics methods. The specific expression patterns of the BrCMF gene in different tissues, as well as the transcriptome and RT-qPCR results under different photoperiodic conditions, were further analyzed. The results showed that BrCMF11 was significantly upregulated in ebm5 under LD conditions, suggesting that BrCMF11 promoted flowering under LD conditions in Chinese cabbage. These findings revealed the function of the BrCCT gene family in photoperiod flowering regulation and provided a prominent theoretical foundation for molecular breeding in Chinese cabbage. Full article

(This article belongs to the Special Issue Optimized Light Management in Controlled-Environment Horticulture)

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18 pages, 5627 KB

Open AccessArticle

The Influence of Bud Positions on the Changes in Carbohydrates and Nitrogen in Response to Hydrogen Cyanamide During Budbreak in Low-Chill Kiwifruit

by Wanichaya Chaiwimol, Wisuwat Songnuan, Hitoshi Ohara, Yotin Juprasong and Aussanee Pichakum

Horticulturae 2025, 11(7), 847; https://doi.org/10.3390/horticulturae11070847 - 17 Jul 2025

Viewed by 1098

Abstract

Climate change has contributed to a decline in winter chilling accumulation, a critical requirement for budbreak in temperate fruit crops. Its consequence has been a reduction in fruit production. To compensate for insufficient chilling, hydrogen cyanamide (HC) is widely applied, though its effectiveness [...] Read more.

Climate change has contributed to a decline in winter chilling accumulation, a critical requirement for budbreak in temperate fruit crops. Its consequence has been a reduction in fruit production. To compensate for insufficient chilling, hydrogen cyanamide (HC) is widely applied, though its effectiveness remains limited. This study investigated the effect of HC application on budbreak in low-chill kiwifruit under warm conditions by correlating phenological responses with changes in carbohydrate and nitrogen concentrations in bark tissues across bud positions. Phenological observations revealed the highest budbreak percentage and total flower buds at the apical position. HC significantly increased budbreak by 58.82% at the apical position and by 375% at the middle position, with corresponding increases in total flower buds by 148.78% and 1066.67%, respectively. Additionally, shoot lengths were uniform among bud positions in HC-treated canes, whereas non-treated canes showed shoot length heterogeneity. Moreover, HC treatment triggered an earlier and more pronounced reduction in soluble sugars (sucrose and hexoses) concentrations along the gradient from apical to basal bud positions, where the response was strongest at the apical position, which was strongly associated with enhanced budbreak percentages and total flower bud formation. While total nitrogen content was highest in the apical position, it was unaffected by HC application. These findings indicate that HC may promote budbreak by enhancing the mobilization and consumption of soluble sugars for bud growth, thereby improving budbreak performance, flower bud production, and uniform shoot development in low-chill kiwifruit under warm conditions. Full article

(This article belongs to the Section Fruit Production Systems)

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29 pages, 1493 KB

Open AccessFeature PaperArticle

Effects of Hydroponic Cultivation on Baby Plant Characteristics of Tetragonia tetragonioides (Pallas) O. Kunze at Harvest and During Storage as Minimally Processed Produce

by Alessandro Esposito, Alessandra Moncada, Filippo Vetrano, Eristanna Palazzolo, Caterina Lucia and Alessandro Miceli

Horticulturae 2025, 11(7), 846; https://doi.org/10.3390/horticulturae11070846 - 17 Jul 2025

Viewed by 403

Abstract

Tetragonia tetragonioides, or New Zealand spinach, is a widespread halophyte native to eastern Asia, Australia, and New Zealand, and naturalized in some Mediterranean regions. This underutilized vegetable is consumed for its leaves, raw or cooked. For the first time, we investigated the [...] Read more.

Tetragonia tetragonioides, or New Zealand spinach, is a widespread halophyte native to eastern Asia, Australia, and New Zealand, and naturalized in some Mediterranean regions. This underutilized vegetable is consumed for its leaves, raw or cooked. For the first time, we investigated the feasibility of using whole baby plants (including stems and leaves) as raw material for ready-to-eat (RTE) vegetable production. Our study assessed Tetragonia’s suitability for hydroponic cultivation over two cycles (autumn–winter and spring). We investigated the impact of increasing nutrient rates (only water, half-strength, and full-strength nutrient solutions) and plant densities (365, 497, and 615 plants m⁻² in the first trial and 615 and 947 plants m⁻² in the second) on baby plant production. We also analyzed the plants’ morphological and biochemical characteristics, and their viability for cold storage (21 days at 4 °C) as a minimally processed product. Tetragonia adapted well to hydroponic cultivation across both growing periods. Nevertheless, climatic conditions, plant density, and nutrient supply significantly influenced plant growth, yield, nutritional quality, and post-harvest storage. The highest plant density combined with the full-strength nutrient solution resulted in the highest yield, especially during spring (1.8 kg m⁻²), and favorable nutritional characteristics (β-carotene, Vitamin C, Fe, Cu, Mn, and Zn). Furthermore, Tetragonia baby plants proved suitable for minimal processing, maintaining good quality retention for a minimum of 14 days, thus resulting in a viable option for the RTE vegetable market. Full article

(This article belongs to the Section Protected Culture)

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19 pages, 5014 KB

Open AccessArticle

Relationship Between Volatile Aroma Components and Amino Acid Metabolism in Crabapple (Malus spp.) Flowers, and Development of a Cultivar Classification Model

by Jingpeng Han, Yuxing Yao, Wenhuai Kang, Yang Wang, Jingchuan Li, Huizhi Wang and Ling Qin

Horticulturae 2025, 11(7), 845; https://doi.org/10.3390/horticulturae11070845 - 17 Jul 2025

Viewed by 353

Abstract

The integration of HS-SPME-GC/MS and UPLC-MS/MS techniques enabled the profiling of volatile organic compounds (VOCs) and amino acids (AAs) in 18 crabapple flower cultivars, facilitating the development of a novel VOC–AA model. Among the 51 identified VOCs, benzyl alcohol, benzaldehyde, and ethyl benzoate [...] Read more.

The integration of HS-SPME-GC/MS and UPLC-MS/MS techniques enabled the profiling of volatile organic compounds (VOCs) and amino acids (AAs) in 18 crabapple flower cultivars, facilitating the development of a novel VOC–AA model. Among the 51 identified VOCs, benzyl alcohol, benzaldehyde, and ethyl benzoate were predominant, categorizing cultivars into fruit-almond, fruit-sweet, and mixed types. The amino acids, namely glutamic acid (Glu), asparagine (Asn), aspartic acid (Asp), serine (Ser), and alanine (Ala) constituted 83.6% of the total AAs identified. Notably, specific amino acids showed positive correlations with key VOCs, suggesting a metabolic regulatory mechanism. The Orthogonal Partial Least Squares Discriminant Analysis (OPLS-DA) model, when combined with volatile organic compounds (VOCs) and amino acid profiles, enabled more effective aroma type classification, providing a robust foundation for further studies on aroma mechanisms and targeted breeding. Full article

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14 pages, 1990 KB

Open AccessArticle

Hierarchic Branch Morphology, Needle Chlorophyll Content, and Needle and Branch Non-Structural Carbohydrate Concentrations (NSCs) Imply Young Pinus koraiensis Trees Exhibit Diverse Responses Under Different Light Conditions

by Bei Li, Wenkai Li, Sudipta Saha, Xiao Ma, Yang Liu, Haibo Wu, Peng Zhang and Hailong Shen

Horticulturae 2025, 11(7), 844; https://doi.org/10.3390/horticulturae11070844 - 17 Jul 2025

Viewed by 341

Abstract

Research on young trees’ adaptation to shade has predominantly focused on leaf-level responses, overlooking critical structural and functional adaptations in branch systems. In this study, we address this gap by investigating hierarchical branch morphology–physiology integration in 20-year-old Pinus koraiensis specimens across four distinct [...] Read more.

Research on young trees’ adaptation to shade has predominantly focused on leaf-level responses, overlooking critical structural and functional adaptations in branch systems. In this study, we address this gap by investigating hierarchical branch morphology–physiology integration in 20-year-old Pinus koraiensis specimens across four distinct light conditions classified by photosynthetic photon flux density (PPFD): three in the understory (low light, LL: 0–25 μmol/m²/s; moderate light, ML: 25–50 μmol/m²/s; and high levels of light, HL: 50–100 μmol/m²/s) and one under full light as a control (FL: 1300–1700 μmol/m²/s). We measured branch base diameter, length, and angle as well as chlorophyll and NSCs content in branches and needles. Branch base diameter and length were more than 1.5-fold higher in the FL Korean pine trees compared to the understory-grown ones, while the branching angle and ratio in the LL Korean pine trees were more than two times greater than those in the FL trees. As light levels increased, Chlorophyll a and b and total chlorophyll (Chla, Chlb, and Chl) concentrations in the needles all significantly decreased. Starch, glucose, and NSC (Starch + Soluble Sugars) concentrations in both needles and branches were the highest in the trees under FL and lowest under ML (except for soluble sugars in branches). Understory young P. koraiensis trees morphologically and physiologically adapt to limited light conditions, growing to be more horizontal, synthesizing more chlorophyll in needles, and attempting to increase their light-foraging ability. We recommend gradually expanding growing spaces to increase light availability for 20-year-old Korean pine trees grown under canopy level. Full article

(This article belongs to the Section Floriculture, Nursery and Landscape, and Turf)

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28 pages, 2881 KB

Open AccessArticle

Segmentation-Based Classification of Plants Robust to Various Environmental Factors in South Korea with Self-Collected Database

by Ganbayar Batchuluun, Seung Gu Kim, Jung Soo Kim and Kang Ryoung Park

Horticulturae 2025, 11(7), 843; https://doi.org/10.3390/horticulturae11070843 - 17 Jul 2025

Viewed by 391

Abstract

Many plant image-based studies primarily use datasets featuring either a single plant, a plant with only one leaf, or images containing only plants and leaves without any background. However, in real-world scenarios, a substantial portion of acquired images consists of blurred plants or [...] Read more.

Many plant image-based studies primarily use datasets featuring either a single plant, a plant with only one leaf, or images containing only plants and leaves without any background. However, in real-world scenarios, a substantial portion of acquired images consists of blurred plants or extensive backgrounds rather than high-resolution details of the target plants. In such cases, classification models struggle to identify relevant areas for classification, leading to insufficient information and reduced classification performance. Moreover, the presence of moisture, water droplets, dust, or partially damaged leaves further degrades classification accuracy. To address these challenges and enhance classification performance, this study introduces a plant image segmentation (Pl-ImS) model for segmentation and a plant image classification (Pl-ImC) model for classification. The proposed models were evaluated using a self-collected dataset of 21,760 plant images captured under real field conditions in South Korea, incorporating various environmental factors such as moisture, water droplets, dust, and partial leaf loss. The segmentation method achieved a dice score (DS) of 89.90% and an intersection over union (IoU) of 81.82%, while the classification method attained an F1-score of 95.97%, surpassing state-of-the-art methods. Full article

(This article belongs to the Special Issue Emerging Technologies in Smart Agriculture)

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13 pages, 4687 KB

Open AccessArticle

Temporary Immersion Bioreactor for In Vitro Multiplication of Raspberry (Rubus idaeus L.)

by Bruno Reyes-Beristain, Eucario Mancilla-Álvarez, José Abel López-Buenfil and Jericó Jabín Bello-Bello

Horticulturae 2025, 11(7), 842; https://doi.org/10.3390/horticulturae11070842 - 17 Jul 2025

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Abstract

Raspberry (Rubus idaeus L.) micropropagation is an alternative for obtaining plantlets with high genetic and phytosanitary quality. The objective of this study was to establish a protocol for the micropropagation of raspberry (Rubus idaeus L.) using the temporary immersion bioreactor, under [...] Read more.

Raspberry (Rubus idaeus L.) micropropagation is an alternative for obtaining plantlets with high genetic and phytosanitary quality. The objective of this study was to establish a protocol for the micropropagation of raspberry (Rubus idaeus L.) using the temporary immersion bioreactor, under intermittent immersion periods and different culture medium volumes. The effect of the liquid medium using the TIB and semisolid was evaluated. Different immersion frequencies and culture medium volumes per explant were evaluated in the TIB. In all treatments, the number of shoots per explant, shoot length, number of leaves per explant, percentage of hyperhydricity, and chlorophyll and β-carotene content at multiplication stage were evaluated. The generated shoots, without a root system, were transferred to the acclimatization stage. The results show that the TIB with an immersion frequency of 2 min every 8 h and a volume of 25 mL of culture medium per explant had the best developmental parameters, with 5.75 shoots per explant, a shoot length of 3.44 cm, and 2% hyperhydricity. The highest chlorophyll and β-carotene content was observed in the TIB at different immersion frequencies of 4, 8 and 12 h, with 25 and 50 mL per explant. Survival percentages higher than 96% were observed in all methods evaluated. In conclusion, the evaluated immersion system is an efficient alternative for R. idaeus micropropagation, without using a rooting stage. Full article

(This article belongs to the Special Issue Tissue Culture and Micropropagation Techniques of Horticultural Crops)

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34 pages, 7027 KB

Open AccessArticle

From Ornamental Beauty to Economic and Horticultural Significance: Species Diversity and Ethnobotany of Bignoniaceae in Maha Sarakham Province, Thailand

by Surapon Saensouk, Piyaporn Saensouk, Thawatphong Boonma, Sarayut Rakarcha, Khamfa Chanthavongsa, Narumol Piwpuan and Tammanoon Jitpromma

Horticulturae 2025, 11(7), 841; https://doi.org/10.3390/horticulturae11070841 - 16 Jul 2025

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Abstract

The Bignoniaceae family encompasses numerous species of ecological, medicinal, and cultural significance, yet its ethnobotanical value remains underexplored in many regions of Thailand. This study investigates the diversity, phenology, cultural relevance, and traditional uses of Bignoniaceae species in Maha Sarakham Province, Northeastern Thailand. [...] Read more.

The Bignoniaceae family encompasses numerous species of ecological, medicinal, and cultural significance, yet its ethnobotanical value remains underexplored in many regions of Thailand. This study investigates the diversity, phenology, cultural relevance, and traditional uses of Bignoniaceae species in Maha Sarakham Province, Northeastern Thailand. Through semi-structured interviews with 260 local informants across 13 districts—alongside field observations and herbarium voucher collections—we documented 27 species across 21 genera. These integrated methods enabled the identification of key culturally significant species and provided insights into their traditional uses. Phenological data revealed clear seasonal patterns in flowering and fruiting, aligned with the regional climatic cycle. Quantitative ethnobotanical indices—including Species Use Value (SUV), Genera Use Value (GUV), Relative Frequency of Citation (RFC), Cultural Importance Index (CI), and Cultural Food Significance Index (CFSI)—were employed to evaluate species significance. Results indicate that species such as Dolichandrone serrulata, D. spathacea, and Oroxylum indicum hold high cultural and practical value, particularly in traditional medicine, spiritual practices, and local landscaping. These findings underscore the critical role of Bignoniaceae in sustaining biocultural diversity and emphasize the urgency of preserving traditional botanical knowledge amid environmental and socio-economic change. Moreover, the insights contribute to broader efforts in cultural heritage preservation and biodiversity conservation across tropical and subtropical regions. Full article

(This article belongs to the Section Medicinals, Herbs, and Specialty Crops)

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20 pages, 41202 KB

Open AccessArticle

Copper Stress Levels Classification in Oilseed Rape Using Deep Residual Networks and Hyperspectral False-Color Images

by Yifei Peng, Jun Sun, Zhentao Cai, Lei Shi, Xiaohong Wu, Chunxia Dai and Yubin Xie

Horticulturae 2025, 11(7), 840; https://doi.org/10.3390/horticulturae11070840 - 16 Jul 2025

Viewed by 353

Abstract

In recent years, heavy metal contamination in agricultural products has become a growing concern in the field of food safety. Copper (Cu) stress in crops not only leads to significant reductions in both yield and quality but also poses potential health risks to [...] Read more.

In recent years, heavy metal contamination in agricultural products has become a growing concern in the field of food safety. Copper (Cu) stress in crops not only leads to significant reductions in both yield and quality but also poses potential health risks to humans. This study proposes an efficient and precise non-destructive detection method for Cu stress in oilseed rape, which is based on hyperspectral false-color image construction using principal component analysis (PCA). By comprehensively capturing the spectral representation of oilseed rape plants, both the one-dimensional (1D) spectral sequence and spatial image data were utilized for multi-class classification. The classification performance of models based on 1D spectral sequences was compared from two perspectives: first, between machine learning and deep learning methods (best accuracy: 93.49% vs. 96.69%); and second, between shallow and deep convolutional neural networks (CNNs) (best accuracy: 95.15% vs. 96.69%). For spatial image data, deep residual networks were employed to evaluate the effectiveness of visible-light and false-color images. The RegNet architecture was chosen for its flexible parameterization and proven effectiveness in extracting multi-scale features from hyperspectral false-color images. This flexibility enabled RegNetX-6.4GF to achieve optimal performance on the dataset constructed from three types of false-color images, with the model reaching a Macro-Precision, Macro-Recall, Macro-F₁, and Accuracy of 98.17%, 98.15%, 98.15%, and 98.15%, respectively. Furthermore, Grad-CAM visualizations revealed that latent physiological changes in plants under heavy metal stress guided feature learning within CNNs, and demonstrated the effectiveness of false-color image construction in extracting discriminative features. Overall, the proposed technique can be integrated into portable hyperspectral imaging devices, enabling real-time and non-destructive detection of heavy metal stress in modern agricultural practices. Full article

(This article belongs to the Special Issue Application of Artificial Intelligence in the Processing of Horticultural Crops)

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12 pages, 2098 KB

Open AccessArticle

A High-Efficiency Transient Expression System Reveals That CjMYB5 Positively Regulates Anthocyanin Biosynthesis in Camellia japonica

by Menglong Fan, Hong Jiang, Si Wu, Zhixin Song, Ying Zhang, Xinlei Li and Yan Wang

Horticulturae 2025, 11(7), 839; https://doi.org/10.3390/horticulturae11070839 - 16 Jul 2025

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Abstract

The establishment of a transient expression system in petals is significant for elucidating gene functions in flowering trees characterized by a prolonged juvenile phase. Genetic improvements in Camellia japonica have been hindered due to the absence of a functional validation platform. In this [...] Read more.

The establishment of a transient expression system in petals is significant for elucidating gene functions in flowering trees characterized by a prolonged juvenile phase. Genetic improvements in Camellia japonica have been hindered due to the absence of a functional validation platform. In this study, we explored an Agrobacterium-mediated and readily observable transient expression system in camellia petals to systematically optimize four critical factors affecting transformation efficiency. As a result, the bud stage, ‘Banliuxiang’ genotype, OD600 of 1.0, and 1-day co-cultivation achieved the highest intensity of transient expression, and overexpression of the Ruby1 reporter gene induced substantial anthocyanin synthesis, manifested as distinct red pigmentation. Furthermore, the optimized transient expression system revealed that the R2R3-MYB transcription factor CjMYB5, which interacted with CjGL3, promoted anthocyanin biosynthesis in camellia petals by transactivating key DFR structural genes. This transient expression platform not only advances functional genomics studies in ornamental woody species but also lays a foundation for molecular breeding programs in C. japonica. Full article

(This article belongs to the Special Issue Germplasm, Genetics and Breeding of Ornamental Plants)

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20 pages, 2048 KB

Open AccessFeature PaperArticle

Effect of Tm-2a, Sw-5 and Ty-1 Gene Introduction on the Agronomic Performance and Metabolic Profile of Traditional Muchamiel-Type Tomato Varieties

by Alicia Sánchez, Juana Cava, Virginia Hernández, Pilar Flores, Santiago García-Martínez, Pedro Carbonell, Elena Sánchez, Nuria López, Elia Molina, José Fenoll and Pilar Hellín

Horticulturae 2025, 11(7), 838; https://doi.org/10.3390/horticulturae11070838 - 15 Jul 2025

Viewed by 432

Abstract

The introduction of virus resistance genes into traditional tomato varieties offers a strategy to preserve genetic diversity and enhance commercial viability. However, the homozygous presence of these genes has been associated with negative effects on yield and fruit quality. This two-year study evaluated [...] Read more.

The introduction of virus resistance genes into traditional tomato varieties offers a strategy to preserve genetic diversity and enhance commercial viability. However, the homozygous presence of these genes has been associated with negative effects on yield and fruit quality. This two-year study evaluated the impact of introducing the Tm-2a, Sw-5 and Ty-1 genes, which are associated with resistance to ToMV, TSWV and TYLCV, respectively, on the agronomic yield, fruit characteristics and metabolic profile of Muchamiel-type cultivars. Four hybrids were obtained by crossing two breeding lines carrying the resistance genes in homozygosis (UMH1139 and UMH1200) with two traditional susceptible varieties (MC1 and MC2). Hybrids matched or exceeded the agronomic performance of their parents. Fruit morphology of the hybrids was similar to traditional parents. The presence of Ty-1 correlated with reduced organic acid concentration, though hybrids exhibited higher levels than the homozygous line, UMH1200. No negative effects on soluble sugars or secondary metabolites were observed. Genotypes carrying resistance genes, breeding lines and hybrids exhibited higher flavonoid contents, suggesting a potential role in virus response. Hybrids maintained or improved the bioactive profile of traditional varieties. These findings support the development of Muchamiel-type hybrids that combine the presence of virus resistance genes in heterozygosity with the desirable traits of traditional tomatoes. Full article

(This article belongs to the Special Issue Genetics, Genomics and Breeding of Vegetable Crops)

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15 pages, 1806 KB

Open AccessFeature PaperArticle

Effects of Nitrogen Application on Soluble Sugar and Starch Accumulation During Sweet Potato Storage Root Formation

by Hong Tham Dong, Yujuan Li, Philip Brown, Delwar Akbar and Cheng-Yuan Xu

Horticulturae 2025, 11(7), 837; https://doi.org/10.3390/horticulturae11070837 - 15 Jul 2025

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Abstract

Nitrogen is an essential element for plant growth, and both insufficient and excessive use of nitrogen have been shown to negatively affect sweet potato production. Nitrogen supply can affect carbon metabolism in plant storage organs; however, limited studies have examined its effects on [...] Read more.

Nitrogen is an essential element for plant growth, and both insufficient and excessive use of nitrogen have been shown to negatively affect sweet potato production. Nitrogen supply can affect carbon metabolism in plant storage organs; however, limited studies have examined its effects on the accumulation of non-structural carbohydrates (soluble sugar and starch) during the formation of sweet potato storage roots. Two pot trials were conducted to evaluate the effects of different nitrogen application levels and timings on the accumulation of non-structural carbohydrates during the formation of sweet potato storage roots. In the first experiment, plants were supplied with 0, 50, 100, or 200 mg/L of nitrogen. In the second experiment, the optimum nitrogen rate (100 mg/L) for storage root formation from the previous experiment was applied at five different times: nil N supply and nitrogen applied at planting or 3, 7, or 14 days after planting. A significant highest starch accumulation in roots during the first 35 days after transplanting was recorded in the 100 mg/L treatment. However, sweet potato required more nitrogen after storage root formation, as indicated by higher non-structural carbohydrate accumulation in roots (1905 mg/plant) in the 200 mg/L treatment at 49 days after planting. Earlier nitrogen applications promoted soluble sugar and starch accumulation in plants during storage root formation, with up to 5697 mg of non-structural carbohydrate accumulated in a plant. The study provided agronomic indicators that moderate nitrogen should be available in soil before or on planting day. Full article

(This article belongs to the Section Plant Nutrition)

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15 pages, 1686 KB

Open AccessArticle

Effect of Sugar Beet Molasses and Compost from Brewery Sludge on Celery (Apium graveolens var. rapaceum) Yield and Nutrient Uptake

by Boris Adamović, Đorđe Vojnović, Ivana Maksimović, Marina Putnik Delić, Dragan Kovačević, Ranko Čabilovski, Milorad Živanov, Maja Ignjatov, Janko Červenski and Dragana Latković

Horticulturae 2025, 11(7), 836; https://doi.org/10.3390/horticulturae11070836 - 15 Jul 2025

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Abstract

The rising cost of mineral fertilizers and the decreasing availability of manure in vegetable farming highlight the need for alternative fertilization strategies. To examine the possibility of applying byproducts from the food processing industry, sugar beet molasses, and compost from brewery sewage sludge [...] Read more.

The rising cost of mineral fertilizers and the decreasing availability of manure in vegetable farming highlight the need for alternative fertilization strategies. To examine the possibility of applying byproducts from the food processing industry, sugar beet molasses, and compost from brewery sewage sludge in celery production, the field experiment was conducted over two years, using a randomized complete block design with three replications. The examined variants were T0—control (without fertilizer); T1—mineral fertilizer; T2—cattle manure; T3—sheep manure; T4—poultry manure; T5—supercompost; and T6—molasses. In the first year, there was no significant difference between T1 and T5 in thickened root yield, while these two variants achieved significantly higher yield compared with other variants. In both years, the highest leaf yield was achieved with T1, while no significant difference was found between T5, T6, and conventional organic fertilizers of animal origin. The highest amount of N was absorbed by roots in T1 (42.0 kg/ha and 51.2 kg/ha, respectively), while the lowest amount was absorbed in T0 (25.5 kg/ha and 26.7 kg/ha, respectively). A significantly higher amount of P₂O₅ was absorbed by roots in all organic fertilizer variants compared to T0 and T1. In both years, of all the nutrients, K₂O was the most absorbed nutrient by the celery root, while CaO was absorbed in greater quantities than N. Based on two years of research, it can be concluded that compost from brewery sludge and sugar beet molasses can be used as an alternative source of nutrients for plants. Full article

(This article belongs to the Section Plant Nutrition)

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Horticulturae, Volume 11, Issue 7 (July 2025) – 154 articles

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