Horticulturae

26 pages, 7176 KiB

Open AccessArticle

Evolutionary Expansion, Structural Diversification, and Functional Prediction of the GeBP Gene Family in Brassica oleracea

by Ziying Zhu, Kexin Ji and Zhenyi Wang

Horticulturae 2025, 11(8), 968; https://doi.org/10.3390/horticulturae11080968 - 15 Aug 2025

Abstract

The GLABROUS1 Enhancer Binding Protein (GeBP) gene family plays a crucial role in plant growth, development, and stress responses. In this study, 28 GeBP genes were identified in Brassica oleracea using HMMER and validated through multiple conserved domain databases. A phylogenetic tree was [...] Read more.

The GLABROUS1 Enhancer Binding Protein (GeBP) gene family plays a crucial role in plant growth, development, and stress responses. In this study, 28 GeBP genes were identified in Brassica oleracea using HMMER and validated through multiple conserved domain databases. A phylogenetic tree was constructed based on the GeBP protein sequences from B. oleracea, Arabidopsis thaliana, Brassica rapa, and Brassica napus, dividing them into four evolutionary clades (A–D), which revealed a close evolutionary relationship within the genus Brassica. Conserved motif and gene structure analyses showed clade-specific features, while physicochemical property analysis indicated that most BoGeBP proteins are hydrophilic, nuclear-localized, and structurally diverse. Gene duplication and chromosomal localization analyses suggested that both segmental and tandem duplication events have contributed to the expansion of this gene family. Promoter cis-element analysis revealed a dominance of light-responsive and hormone-responsive elements, implying potential roles in photomorphogenesis and stress signaling pathways. Notably, the protein encoded by BolC01g019630.2J possesses both a transmembrane domain and characteristics of the Major Facilitator Superfamily (MFS) transporter family, and it is predicted to localize to the plasma membrane. This suggests that it may act as a molecular bridge between environmental signal perception and transcriptional regulation, potentially representing a novel signaling mechanism within the GeBP family. This unique feature implies its involvement in transmembrane signal perception and downstream transcriptional regulation under environmental stimuli, providing valuable insights for further investigation of its role in stress responses and metabolic regulation. Overall, this study provides a theoretical foundation for understanding the evolutionary patterns and functional diversity of the GeBP gene family in B. oleracea and lays a basis for future functional validation and breeding applications. Full article

(This article belongs to the Section Genetics, Genomics, Breeding, and Biotechnology (G2B2))

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22 pages, 1492 KiB

Open AccessFeature PaperArticle

Foliar Application of Iron and Zinc Affected Aromatic Plants Grown Under Conventional and Organic Agriculture Differently

by Nikolaos Tzortzakis, Efraimia Hajisolomou, Nikoletta Zaravelli and Antonios Chrysargyris

Horticulturae 2025, 11(8), 967; https://doi.org/10.3390/horticulturae11080967 - 14 Aug 2025

Abstract

The utilization of organic fertilizers for the cultivation of wild edible and medicinal plants offers agronomic and ecological benefits, given their suitability to low-input and sustainable production systems. Under such conditions, these species may also benefit from targeted foliar applications of micronutrients to [...] Read more.

The utilization of organic fertilizers for the cultivation of wild edible and medicinal plants offers agronomic and ecological benefits, given their suitability to low-input and sustainable production systems. Under such conditions, these species may also benefit from targeted foliar applications of micronutrients to enhance their nutritional quality. This study examined the effects of a vinasse-based organic fertilizer and conventional fertilization regime, in combination with foliar applications of iron (Fe) and zinc (Zn), on the biomass, leaf photochemistry, and plant stress-related responses of Sideritis cypria and Origanum dubium. In S. cypria, organic fertilization resulted in a similar yield compared to conventional fertilization, while O. dubium showed a significant decrease in yield when using organic fertilizers. The impact of spraying with Zn on S. cypria dry matter content was related to the availability of nutrients, particularly nitrogen, while in O. dubium Zn spraying induced a decrease in dry matter. The total phenols content and antioxidant activity of S. cypria were elevated by conventional fertilization and foliar application of Fe, while the combination of organic fertilization and foliar application of Fe and Zn reduced lipid peroxidation. In O. dubium, foliar application of Fe and Zn led to a reduction in total phenols content, antioxidant capacity, and hydrogen peroxide content under adequate nutrition. In general, foliar spraying with Zn tended to improve water use efficiency under specific fertilization practices on both species, while the positive effect of conventional fertilization on nutrient use efficiency still requires further validation. Ultimately, the efficiency of organic fertilization was related to the examined species, inducing variations in leaf chlorophyll content. In addition, foliar application of Fe and Zn affected the antioxidant capacity and mineral content of the examined species. Thus, appropriate fertilization management is vital to fully realize the specific benefits of foliar micronutrient addition. Full article

(This article belongs to the Special Issue Wild Plant Species as Potential Horticultural Crops: An Opportunity for Farmers and Consumers—2nd Edition)

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18 pages, 4340 KiB

Open AccessArticle

Comparing Two Varieties of Blood Orange: A Differential Methylation Region Within the Specific Encoding Sequence of a Retrotransposon Adjacent to the Ruby Locus

by Jianhui Wang, Zhihong Li, Weiqing Guo, Zhihan Liu, Mingfu Xu, Yan Sun, Dayu Liu and Ying Chen

Horticulturae 2025, 11(8), 966; https://doi.org/10.3390/horticulturae11080966 - 14 Aug 2025

Abstract

The blood orange arose from the insertion of a retrotransposon adjacent to the Ruby gene, an MYB-type transcriptional activator of anthocyanin production, as reported previously. However, the intricate process of anthocyanin regulation among different varieties of blood orange remains incompletely understood. In this [...] Read more.

The blood orange arose from the insertion of a retrotransposon adjacent to the Ruby gene, an MYB-type transcriptional activator of anthocyanin production, as reported previously. However, the intricate process of anthocyanin regulation among different varieties of blood orange remains incompletely understood. In this study, mRNA levels of the transcription factors Ruby and TT8 were found to be upregulated in the juice vesicle tissues of a variety with higher concentrations of anthocyanins in the pulp compared with another variety with a lower anthocyanin content. In contrast, comparative analysis of the two varieties using two-dimensional electrophoresis and mass spectrometry did not identify differentially expressed proteins related to anthocyanin biosynthesis in the juice vesicle tissues. Furthermore, higher anthocyanin contents were observed in various tissues of transgenic Arabidopsis thaliana overexpressing the Ruby gene from blood orange compared with the wildtype plant. Moreover, the long terminal repeat (LTR) region of a retrotransposon inserted upstream of the Ruby locus exhibited the ability to drive reporter expression through histochemical assay in a transgenic seedling. Thus, a PCR-based molecular marker was developed, targeting the upstream sequence of the Ruby locus to identify Citrus hybrids with the unique trait of red-fleshed fruit. Intriguingly, bisulfite sequencing revealed differentially methylated regions within a Gag-Pol polyprotein-encoding sequence of a retrotransposon adjacent to Ruby locus when comparing two varieties with different anthocyanin contents. A higher average level of methylation status was observed in the fruit with a lower anthocyanin content. In conclusion, methylation modifications at specific upstream positions on the Ruby locus may influence anthocyanin production in blood oranges. Full article

(This article belongs to the Section Developmental Physiology, Biochemistry, and Molecular Biology)

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23 pages, 6953 KiB

Open AccessArticle

Chayote [Sechium edule (Jacq.) Sw.] Fruit Quality Influenced by Plant Pruning

by Jorge Cadena-Iñiguez, Ma. de Lourdes Arévalo-Galarza, Juan F. Aguirre-Medina, Carlos H. Avendaño-Arrazate, Daniel A. Cadena-Zamudio, Jorge David Cadena-Zamudio, Ramón M. Soto-Hernández, Víctor M. Cisneros-Solano, Lucero del Mar Ruiz-Posadas, Celeste Soto-Mendoza and Jorge L. Mejía-Méndez

Horticulturae 2025, 11(8), 965; https://doi.org/10.3390/horticulturae11080965 - 14 Aug 2025

Abstract

Plant pruning is the selective removal of specific plant parts to enhance growth, shape, and health. In this work, the effects of pruning were evaluated regarding the physiological parameters, maturity, quality, and harvest indices and the nutritional quality features of twelve chayote [ [...] Read more.

Plant pruning is the selective removal of specific plant parts to enhance growth, shape, and health. In this work, the effects of pruning were evaluated regarding the physiological parameters, maturity, quality, and harvest indices and the nutritional quality features of twelve chayote [Sechium edule (Jacq.) Sw] (Cucurbitaceae) varieties. GC-FID approaches were utilized to determine CO₂ assimilation rates. The results demonstrated that pruning upregulated the leaf temperature and conductance but decreased transpiration and CO₂ assimilation rates within the evaluated period (06:30 a.m.–16:23 p.m.). It was noted that the implementation of pruning also impacted samples with enhanced photosynthetically active radiation activity, with a positive correlation with CO₂ assimilation. The macro- and micronutrient content was higher in samples with an epidermis, especially for S. edule var. albus spinosum. Nevertheless, the analyzed samples presented low (5–10 mL CO₂ kg⁻¹ h⁻¹), medium (10–15 mL CO₂ kg⁻¹ h⁻¹), and high levels (15–20 mL CO₂ kg⁻¹ h⁻¹) of respiratory intensity and weight loss (7–17%)—effects attributed to botanical differences between the studied chayote varieties. This work demonstrates, for the first time, the effects of pruning in chayote orchards and expands the knowledge regarding the implementation of effective approaches to produce plants with culinary, cultural, and medicinal implications. Further approaches are required to determine the effects of pruning on chayote after harvest. Full article

(This article belongs to the Special Issue New Strategies for Agrochemical-Conventional Management in Horticultural Crops)

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11 pages, 1967 KiB

Open AccessArticle

Exogenous Melatonin Affects Fruit Enlargement and Sugar Metabolism in Melt Peach

by Yanfei Guo, Baoxin Jiang, Qinghao Wang, Huilian Xu and Wangshu Zhang

Horticulturae 2025, 11(8), 964; https://doi.org/10.3390/horticulturae11080964 - 14 Aug 2025

Abstract

Peach (Prunus persica (L.)) fruits are abundant in nutrients, with fruit shape and sugar content serving as critical indicators of fruit quality. Melatonin plays a pivotal role in peach fruit development; however, the mechanisms by which it regulates fruit shape development, sugar [...] Read more.

Peach (Prunus persica (L.)) fruits are abundant in nutrients, with fruit shape and sugar content serving as critical indicators of fruit quality. Melatonin plays a pivotal role in peach fruit development; however, the mechanisms by which it regulates fruit shape development, sugar metabolism, and secondary metabolites remain largely unknown. In this study, peach trees were sprayed with 150 µM melatonin 20 days after pollination. Traditional methods were used to investigate fruit morphology, total soluble solids (TSSs), and titratable acidity content (TAC), while liquid chromatography–mass spectrometry (LC-MS) was employed to analyze sugar metabolites during fruit development. The results indicated that melatonin treatment augmented the transverse and longitudinal diameters of peach fruits by 12% and 6%, respectively, and elevated the contents of soluble solids and titratable acid by 7% and 6%, respectively. The single fruit weight experienced a significant increase of 29.4%, whereas fruit firmness at maturity remained unchanged. Metabolite analysis demonstrated that melatonin decreased the levels of sucrose and D-sorbitol in mature fruits but enhanced the accumulation of D-fructose, L-rhamnose, and xylose. Significantly, melatonin expedited the degradation of galactose, D-mannose, and methyl-D-pyranogalactoside prior to maturity (all three substances naturally decline with fruit ripening), highlighting its role in promoting fruit ripening. In conclusion, exogenous melatonin improves the internal nutrition and flavor quality of fruit by regulating the accumulation of primary and secondary metabolites during fruit ripening. Specifically, the increase in D-fructose (a major contributor to sweetness) and L-rhamnose (a potential precursor for aroma compounds) enhances fruit flavor profile. The accelerated degradation of galactose, D-mannose, and methyl-D-pyranogalactoside (components of cell wall polysaccharides) prior to maturity, alongside the metabolic shift favoring fructose accumulation over sucrose, highlights melatonin’s role in promoting fruit ripening and softening processes. It also promotes fruit enlargement and single fruit weight without affecting fruit firmness. This study establishes a theoretical basis for the further investigation of the molecular mechanisms underlying melatonin’s role in peach fruits and for enhancing quality-focused breeding practices. Full article

(This article belongs to the Section Postharvest Biology, Quality, Safety, and Technology)

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22 pages, 2695 KiB

Open AccessArticle

Supplemental Light Differentially Regulates Indoor-Grown Basil (Ocimum basilicum) Growth, Volatile Compounds, and Sensory Attributes

by Nathan Kelly, Madison A. Oehler, Regina O’Brien, Eunhee Park, Jinhe Bai, Jorge M. Fonseca and Yaguang Luo

Horticulturae 2025, 11(8), 963; https://doi.org/10.3390/horticulturae11080963 - 14 Aug 2025

Abstract

Basil (Ocimum basilicum), a widely cultivated herb, thrives in controlled environment agriculture (CEA) systems where light spectra can be precisely manipulated to optimize growth, morphology, and chemical composition. This study examined the effects of supplemental blue (BL), green (GR), and far-red [...] Read more.

Basil (Ocimum basilicum), a widely cultivated herb, thrives in controlled environment agriculture (CEA) systems where light spectra can be precisely manipulated to optimize growth, morphology, and chemical composition. This study examined the effects of supplemental blue (BL), green (GR), and far-red (FR) light on two basil cultivars, green Prospera and purple Amethyst, focusing on plant growth, photosynthetic efficiency, volatile compound profiles, and sensory attributes. The results showed that FR light significantly increased stem elongation and biomass accumulation, with stem height increasing by 66.3% in Prospera and 144.1% in Amethyst under FR light compared to white light, and fresh biomass increasing by 59.3% and 120.1%, respectively. However, FR light increased photosystem II (PSII) efficiency by 20.9% and 34.3% in Prospera and Amethyst, respectively, compared to high-intensity white light, indicating FR light’s impactful role on growth and photosynthetic performance. The volatile profiles were also significantly influenced by light treatments. FR light increased citral levels by 371.0% in Prospera, while allo-ocimene levels increased by 89.0% in Amethyst compared to the control. Sensory evaluations confirmed that basil grown under FR light had a stronger aromatic profile. In contrast, BL light reduced concentrations of certain volatiles, such as eugenol and linalool, leading to a milder aroma. These findings demonstrate that the light spectra can be strategically manipulated to optimize basil’s growth, morphology, and aromatic profile, aligning production with consumer preferences. Full article

(This article belongs to the Section Protected Culture)

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17 pages, 1105 KiB

Open AccessReview

Exploring Factors Influencing the Consumption of Grape Skins: A Review

by Si-Yuan Wan, You-Mei Li and Zhao-Sen Xie

Horticulturae 2025, 11(8), 962; https://doi.org/10.3390/horticulturae11080962 - 14 Aug 2025

Abstract

Grapes (Vitis vinifera L.) are one of the most popular fruits globally, with studies demonstrating the numerous beneficial metabolic substances found in their skins, including anthocyanins, proanthocyanidins, stilbene (resveratrol) and so on. However, grape skins are often overlooked and discarded by consumers. [...] Read more.

Grapes (Vitis vinifera L.) are one of the most popular fruits globally, with studies demonstrating the numerous beneficial metabolic substances found in their skins, including anthocyanins, proanthocyanidins, stilbene (resveratrol) and so on. However, grape skins are often overlooked and discarded by consumers. To maximize the nutritional benefits of grapes, it is crucial to explore the factors influencing the edibility of grape skin and work towards enhancing consumption. This review explores the molecular mechanisms underlying these factors, focusing on cell wall modifications and tannin biosynthesis. We highlight how skin texture and astringency are influenced by enzymatic activity, genetic regulation, and environmental factors. Understanding these mechanisms provides a foundation for improving skin palatability through breeding or biotechnological approaches, enhancing the nutritional and commercial value of grapes. Full article

(This article belongs to the Special Issue Research Progress on Grape Genetic Diversity)

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22 pages, 23775 KiB

Open AccessArticle

Proximal and Remote Sensing Monitoring of the ‘Spinoso sardo’ Artichoke Cultivar on Organic and Conventional Management

by Alessandro Deidda, Alberto Sassu, Luca Ghiani, Maria Teresa Tiloca, Luigi Ledda, Marco Cossu, Paola A. Deligios and Filippo Gambella

Horticulturae 2025, 11(8), 961; https://doi.org/10.3390/horticulturae11080961 - 14 Aug 2025

Abstract

The development of new techniques to improve crop management, especially through precision agriculture methods and innovations, is crucial for increasing crop yield and ensuring high-quality production. The horticultural sector is particularly vulnerable to inefficiencies in crop management due to the complex and costly [...] Read more.

The development of new techniques to improve crop management, especially through precision agriculture methods and innovations, is crucial for increasing crop yield and ensuring high-quality production. The horticultural sector is particularly vulnerable to inefficiencies in crop management due to the complex and costly processes required for producing marketable products. Optimal nutritional inputs and effective disease management are crucial for maintaining commercial standards. This two-year study investigated the physiological differences between organic and conventional crop management of the Sardinian `Spinoso sardo’ artichoke ecotype (Cynara cardunculus var. scolymus L.) by integrating a multiplex force-A (MFA) fluorometer and unmanned aerial systems (UASs) equipped with a multispectral camera capable of analysing the NDVI vegetation index. Using both proximal and remote sensing instruments, physiological and nutritional variations in the growth cycle of artichokes were identified, distinguishing between traditional and two organic management practices. The two-year MFA experiment revealed physiological variability and different trends among the three management practices, indicating that MFA proximal sensing is a valuable tool for detecting physiological differences, particularly in chlorophyll activity and nitrogen content. In contrast, the UAS survey was less effective at distinguishing between management types, likely due to its limited use during the second year and the constrained timeframe of the multitemporal analysis. The analysis of the MFA fluorimetric indices suggested significant differences among the plots monitored due to the ANOVA statistical analysis and Tukey test, showing greater adaptability of the conventional system in managing production inputs, unlike the organic systems, which showed higher variability within the plots and across the survey years, indicating aleatory trends due to differences in crop management. Full article

(This article belongs to the Special Issue Advances in Sustainable Cultivation of Horticultural Crops)

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21 pages, 4415 KiB

Open AccessArticle

Genome-Wide Identification and Characterization of Universal Stress Protein (USP) Family Members in Lycium barbarum and Transcriptional Pattern Analysis in Response to Salt Stress

by Jintao Lu, Mengyao Bai, Jianhua Zhao, Dong Meng, Shanzhi Lin, Yu Xiu and Yuchao Chen

Horticulturae 2025, 11(8), 960; https://doi.org/10.3390/horticulturae11080960 - 14 Aug 2025

Abstract

Lycium barbarum is a traditional medicinal and edible plant species in China, exhibiting notable salt tolerance that enables cultivation in salt-affected soils. However, intensifying soil salinization has rendered severe salt stress a critical limiting factor for its fruit yield and quality. Universal stress [...] Read more.

Lycium barbarum is a traditional medicinal and edible plant species in China, exhibiting notable salt tolerance that enables cultivation in salt-affected soils. However, intensifying soil salinization has rendered severe salt stress a critical limiting factor for its fruit yield and quality. Universal stress proteins (USPs) serve as crucial regulators for plant abiotic stress responses through developmental process modulation. Nevertheless, the characteristics and functional divergence of USP gene family members remain unexplored in L. barbarum. Here, we performed genome-wide identification and characterization of the USP gene family in L. barbarum, revealing 52 members unevenly distributed across all 12 chromosomes. Phylogenetic analysis classified these LbUSP members into four distinct groups, demonstrating the integration of the conserved USP domain and diverse motifs within each group. Collinearity analysis indicated a stronger synteny of LbUSPs with orthologs in Solanum lycopersicum than with other species (Arabidopsis thaliana, Vitis vinifera, and Oryza sativa), demonstrating that gene duplication coupled with functional conservation represented the primary mechanism underlying USP family expansion in L. barbarum. In silico promoter screening detected abundant cis-acting elements associated with abiotic/biotic stress responses (MYB and MYC binding sites), phytohormone regulation (ABRE motif), and growth/development processes (Box-4 and G-box). Transcriptome sequencing and RT-qPCR validation revealed tissue-specific differential expression patterns of LbUSP8, LbUSP11, LbUSP12, LbUSP23, and LbUSP25 in roots and stems under salt stress, identifying them as prime candidates for mediating salt resistance in L. barbarum. Our findings establish a foundation for the functional characterization of LbUSPs and molecular breeding of salt-tolerant L. barbarum cultivars. Full article

(This article belongs to the Special Issue New Insights into Protected Horticulture Stress)

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14 pages, 19171 KiB

Open AccessArticle

Effects of Cardboard Box Ventilation Hole Size During Forced-Air Precooling on Postharvest Quality and Physiological Properties in Cut Roses

by Ruifeng Gu, Jie Bai, Jiawei Sun, Lei Li, Xuan Wang, Huijun Yan, Hao Zhang, Wensheng Wang, Junping Gao and Xiaoming Sun

Horticulturae 2025, 11(8), 959; https://doi.org/10.3390/horticulturae11080959 - 14 Aug 2025

Abstract

Forced-air cooling (FAC) is a method for rapidly reducing the temperature of horticultural products. However, its effects on the physiological properties and quality of cut flowers remain elusively unclear. This study investigated the impact of FAC with different vent hole diameters (4, 8, [...] Read more.

Forced-air cooling (FAC) is a method for rapidly reducing the temperature of horticultural products. However, its effects on the physiological properties and quality of cut flowers remain elusively unclear. This study investigated the impact of FAC with different vent hole diameters (4, 8, and 12 cm) on multiple metabolic pathways and the quality of cut rose flowers. Compared with controls with a conventional slow cooling method, FAC using 8 cm vent holes (FAC8) prolonged the vase life of cut roses by 3 days and reduced Botrytis cinerea incidence by 60%. The data revealed that FAC8 suppressed excessive transpiration in the late vase stages while it enhanced water uptake throughout the vase period. Additionally, FAC8 reduced the respiratory rate in cut roses, decreasing cumulative respiration by 15% versus controls. When detached leaves from cut roses were subjected to water loss treatment, FAC8 induced tighter stomatal closure, resulting in a 33% smaller stomatal aperture than that of controls after 2 h. Correlation analysis of measured indices demonstrated that FAC significantly contributed to the improvement of postharvest quality (p < 0.05) via the regulation of physiological properties. In conclusion, FAC enhances the postharvest quality of cut roses by maintaining stomatal regulatory ability. Full article

(This article belongs to the Special Issue Pre/Post-harvest Treatments to Improve Quality and Longevity of Cut Flowers)

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18 pages, 3123 KiB

Open AccessArticle

Identification of Tomato SET Domain Group Gene Family and Function Analysis Under Temperature Stress

by Chuanlong Lu, Yuan Cheng, Hongjian Wan, Zhuping Yao, Meiying Ruan, Rongqing Wang, Qingjing Ye, Guozhi Zhou, Huasen Wang and Chenxu Liu

Horticulturae 2025, 11(8), 958; https://doi.org/10.3390/horticulturae11080958 - 13 Aug 2025

Abstract

Histone methylation plays important roles in plant development and adaptation to multiple stresses. SET domain group (SDG) proteins are identified as plant histone lysine methyltransferases in Arabidopsis and other crops. However, the SDG gene family and its functional roles in tomato remain unknown. [...] Read more.

Histone methylation plays important roles in plant development and adaptation to multiple stresses. SET domain group (SDG) proteins are identified as plant histone lysine methyltransferases in Arabidopsis and other crops. However, the SDG gene family and its functional roles in tomato remain unknown. In this research, 48 tomato SDG (SlSDG) gene family members were identified, and their chromosomal locations and conserved motifs were determined. According to phylogenetic analysis, the SlSDGs are divided into seven groups, which is consistent with Arabidopsis and rice. Promoter analysis indicated that the SlSDGs may be associated with biotic and abiotic stress responses. The expression pattern of SlSDGs illustrates that heat and cold stress significantly influence the transcript abundance of SDG14/19/21/23/48. The results of a VIGS assay showed that silencing SlSDG19 and SlSDG48 decreases tomato heat tolerance, while silencing SlSDG14 improves the heat tolerance of tomato plants. The analysis of downstream regulating genes indicated that heat shock proteins (HSPs), especially HSP70 and HSP90, act as critical effectors. Similarly, the experimental assay and expression analysis suggest that SDG21 and SDG23 positively and negatively regulate tomato cold tolerance through the CBF-COR pathway, respectively. These findings clarify the function of tomato SDG proteins and provide insight for the genetic improvement of tomato for temperature stress tolerance. Full article

(This article belongs to the Special Issue Advancements in Genetic Improvement of Stress Tolerance in Vegetable Crops)

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26 pages, 3521 KiB

Open AccessArticle

Salinity Tolerance of Novel and Established Olive (Olea europaea L.) Cultivars for Super-High-Density Systems

by Xavier Rius-García, María Videgain-Marco, José Casanova-Gascón, Luis Acuña-Rello and Pablo Martín-Ramos

Horticulturae 2025, 11(8), 957; https://doi.org/10.3390/horticulturae11080957 - 13 Aug 2025

Abstract

The olive industry is transitioning from traditional to super-high-density (SHD) systems to optimize production costs and address labor shortages. This shift coincides with increasing challenges from soil salinization and deteriorating irrigation water quality. This study evaluated salinity tolerance in three novel olive cultivars [...] Read more.

The olive industry is transitioning from traditional to super-high-density (SHD) systems to optimize production costs and address labor shortages. This shift coincides with increasing challenges from soil salinization and deteriorating irrigation water quality. This study evaluated salinity tolerance in three novel olive cultivars (Lecciana, Coriana, and Sikitita) against the established SHD references Arbequina and Arbosana under controlled greenhouse conditions over five months with increasing NaCl concentrations (25, 50, and 75 mM). The analysis revealed distinct adaptation mechanisms among cultivars. Arbosana exhibited balanced tolerance across parameters, with minimal biomass reduction and remarkable photosynthetic resilience. Lecciana demonstrated superior ion regulation, maintaining the highest K⁺/Na⁺ ratios across all salinity levels despite pronounced shoot growth sensitivity at high salinity. Sikitita showed moderate tolerance through biomass maintenance but with significant photosynthetic sensitivity under stress. Arbequina displayed effective chloride exclusion and consistent shoot growth despite biomass sensitivity, whereas Coriana presented notable biomass increases at moderate salinity but poor ion discrimination. Tissue-specific analysis revealed common compartmentalization patterns across cultivars, with roots accumulating the highest Na⁺ and Cl⁻ concentrations. These data identify Arbosana and Lecciana as promising candidates for salinized SHD orchards. Full article

(This article belongs to the Special Issue Olive Stress Alleviation Strategies)

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15 pages, 7173 KiB

Open AccessArticle

Sucrose Transporter 2 Knockout Increases Sugar Content in Tomato Fruits

by Pingfei Ge, Ying Wang, Yuyang Cao, Fangman Li, Xingyu Zhang, Haobo Xu, Yang Yang, Ziyuan Wang, Junshen Lin, Pengyu Zhu and Yuyang Zhang

Horticulturae 2025, 11(8), 956; https://doi.org/10.3390/horticulturae11080956 - 13 Aug 2025

Viewed by 63

Abstract

Sugar content is pivotal in determining the flavor quality of tomato, and numerous genes related to tomato fruit quality have been identified. The distribution of sugar sources in plants primarily relies on the functionality of sugar transporters. Despite this, the specific role of [...] Read more.

Sugar content is pivotal in determining the flavor quality of tomato, and numerous genes related to tomato fruit quality have been identified. The distribution of sugar sources in plants primarily relies on the functionality of sugar transporters. Despite this, the specific role of SUT2, a sucrose transporter family member, in sugar accumulation within tomato fruits is still unclear. This study demonstrates that SUT2 is localized to the plasma membrane and possesses the function of transporting sucrose from the extracellular side to the intracellular side of the plasma membrane. Its expression level progressively decreases during fruit development. SUT2 knockout resulted in a significant increase in sugar content in tomato fruits. Further investigation revealed that the elevated sugar levels in knockout lines were accompanied by alterations in the expression of the sugar accumulation related genes STP1 and CDPK26/27. These findings provide new insights into the biological role of SUT2 in regulating sugar content in tomato fruits, improve our understanding of sugar accumulation mechanisms in tomato fruits, and offer valuable perspectives for quality improvement in tomato. Full article

(This article belongs to the Special Issue Breeding by Design: Advances in Vegetables)

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12 pages, 510 KiB

Open AccessReview

Emerging Ornamental Plant Diseases and Their Management Trends in Northern Italy

by Maria Lodovica Gullino, Domenico Bertetti, Massimo Pugliese and Angelo Garibaldi

Horticulturae 2025, 11(8), 955; https://doi.org/10.3390/horticulturae11080955 - 13 Aug 2025

Viewed by 60

Abstract

The ornamental plant sector is characterized by the production of a large variety of genera, species and cultivars that are much more numerous than those of other agricultural production sectors. Many countries throughout the world are involved in an intensive exchange of potted [...] Read more.

The ornamental plant sector is characterized by the production of a large variety of genera, species and cultivars that are much more numerous than those of other agricultural production sectors. Many countries throughout the world are involved in an intensive exchange of potted plants, cut flowers and propagation material. This intense trade exchange favors the introduction of the causal agents of new diseases on farms, in parks, along tree-lined avenues and in city gardens. Global warming can favor plant pathogens that thrive under high temperatures. Moreover, the interaction between the ongoing increase in temperature and in the CO₂ concentration has caused a significant increase in the disease severity of many pathosystems. The numerous reports of new plant pathogens on ornamental plants in Italy in recent years fall into this context. In plant pathology research, living labs incorporate the complexities and variability of natural conditions, and they can thus be used to conduct experiments and test hypotheses. A private garden, located in the hamlet of Bariola (Piedmont, Biella province, northern Italy), has become an ideal living lab that is used to monitor the evolution of the phytosanitary situation of ornamental plants. The results obtained in this living lab are reported hereafter. Moreover, new trends in disease prevention and management, such as the adoption of appropriate prevention practices, water and fertilization management and use of environmentally friendly methods to reduce pesticide use as part of an integrated pest management approach, are also examined. Full article

(This article belongs to the Special Issue Physiological and Molecular Biology of Ornamental Plants—2nd Edition)

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21 pages, 5914 KiB

Open AccessArticle

Integrated Assays and Microscopy to Study the Botrytis cinerea–Strawberry Interaction Reveal Tissue-Specific Stomatal Penetration

by Lorena Rodriguez Coy, Donovan Garcia-Ceron, Scott W. Mattner, Donald M. Gardiner and Anthony R. Gendall

Horticulturae 2025, 11(8), 954; https://doi.org/10.3390/horticulturae11080954 - 12 Aug 2025

Viewed by 152

Abstract

Strawberry (Fragaria x ananassa) production has increased around the world, but crop quality and yield are threatened by fungal pathogens. Botrytis cinerea is a filamentous fungus that infects over 1400 species of crops, causing gray mold disease with devastating losses to [...] Read more.

Strawberry (Fragaria x ananassa) production has increased around the world, but crop quality and yield are threatened by fungal pathogens. Botrytis cinerea is a filamentous fungus that infects over 1400 species of crops, causing gray mold disease with devastating losses to horticulture worldwide, including strawberry. The heavy reliance on synthetic fungicides in the strawberry industry has led to the emergence of fungicide resistance in B. cinerea. Therefore, understanding the fundamental biology of B. cinerea is an important step in the search for novel antifungals. Although B. cinerea is one of the most serious pathogens of strawberry, this pathosystem is understudied compared to other plant hosts. Consequently, further evidence is needed on pathogen penetration and early disease development in strawberry tissues. Here, we adapted and advanced assays using detached strawberry leaves, fruits, and petals to study B. cinerea infection. These assays allow the comparison of the treatment effect on the same fruit, avoiding confounding from differential ripening, and facilitate the screening of fungicides or biocontrol agents. Through chlorophyll fluorescence analysis and scanning electron and confocal microscopy, we quantified lesions caused by B. cinerea in the early stages of infection in fruit and petals, and demonstrated that B. cinerea penetrates through the stomata of strawberry achenes, revealing a previously unrecognized infection route in this host. These data provide a deeper understanding of the B. cinerea–strawberry interaction and will serve as a foundation for future studies seeking novel antifungal treatments against B. cinerea. Full article

(This article belongs to the Special Issue Fungal Diseases in Horticultural Crops)

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22 pages, 2293 KiB

Open AccessArticle

Effect of the Combined Application of Aqueous Cabbage Seed Extract and Chitosan Solutions on the Shelf Life of Fresh-Cut Apple Cubes

by Despina Alexaki, Athanasios Gerasopoulos and Dimitrios Gerasopoulos

Horticulturae 2025, 11(8), 953; https://doi.org/10.3390/horticulturae11080953 - 12 Aug 2025

Viewed by 182

Abstract

Enzymatic browning is the negative color effect of polyphenol oxidase activity in cut fresh fruit products, which reduces their quality, shelf life, and marketability. To preserve the color after cutting, apple cubes were treated with aqueous cabbage seed extracts (ACEs) at 5–10% w [...] Read more.

Enzymatic browning is the negative color effect of polyphenol oxidase activity in cut fresh fruit products, which reduces their quality, shelf life, and marketability. To preserve the color after cutting, apple cubes were treated with aqueous cabbage seed extracts (ACEs) at 5–10% w:v seed–water ratios, adjusted to pH 4.0 and 6.0 and 1% chitosan added to the ACE before preservation at 7 °C for 0–10 days. Chromatometric readings (L*, a*, and b*) and visual color score were used for shelf life calculation. The ACE total phenolics and glucosinolate levels showed differences among the 5–10% and control groups. Based on color score, uncoated or coated (chitosan or ACE combined with chitosan) apple cubes reached marketing limit levels (score > 3/5) on day one, but apple cubes treated with 5 or 10% ACE alone did so on day four, which was considered the effective shelf life. These findings were further supported by FT-IR analysis. ACE modification to pH 6.0 was more effective at keeping the natural cut apple color than pH 4.0. ACE treatment (at 5 or 10%) without coating is regarded as a very promising natural agent for extending the shelf life of fresh-cut apples, which is a key attribute in their marketing. Full article

(This article belongs to the Special Issue Sustainable Practices to Improve Bioactive Compounds in Horticultural Crops)

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22 pages, 2851 KiB

Open AccessArticle

Propagation and Long-Term Storage of Rhaponticum carthamoides Under In Vitro Conditions

by Olesya Raiser, Damelya Tagimanova, Ainur Turzhanova, Saule Magzumova, Gulden Nagmetova, Zhanar Akhmetkarimova, Nataliya Premina, Nadezhda Filippova and Oxana Khapilina

Horticulturae 2025, 11(8), 952; https://doi.org/10.3390/horticulturae11080952 - 12 Aug 2025

Viewed by 188

Abstract

Rhaponticum carthamoides (Willd.) Iljin. (Leuzea carthamoides, Maral root), a medicinally valuable species listed in the Red Book of Kazakhstan, is known for its rich phytochemical profile. However, limited data exist on its microclonal propagation. This study aimed to optimize in vitro [...] Read more.

Rhaponticum carthamoides (Willd.) Iljin. (Leuzea carthamoides, Maral root), a medicinally valuable species listed in the Red Book of Kazakhstan, is known for its rich phytochemical profile. However, limited data exist on its microclonal propagation. This study aimed to optimize in vitro and medium-term storage conditions using biotechnological methods. Mature seeds collected from natural populations in the Kazakhstani Altai were germinated, and tissues from the seedlings were used as explants. Sterile shoots were cultured on Murashige and Skoog (MS) medium supplemented with 3.0 mg L⁻¹ −6-benzylaminopurine and 3.0 mg L⁻¹ kinetin. For shoot induction, MS medium supplemented with 0.5 mg L⁻¹ meta-Topolin and using stem apices as explants yielded optimal results. Medium-term storage with chlorocholine chloride at 0.1–0.4 g/L effectively preserved regenerative capacity for further rooting. After 12 months of storage, plantlets were transferred to half-strength MS medium with 3.0 g/L activated carbon and at 2.0 or 5.0 mg L⁻¹ indole-3-butyric acid for rooting. Regenerated plants were successfully acclimatized ex vitro. The 20-hydroxyecdysone content in field-grown plants post-storage reached 9.24 mg/mL, 2.4-fold higher than in wild plants. Inter simple sequence repeat analysis confirmed genetic stability. Our optimized protocol ensures high-yield metabolite production and genetic fidelity, enabling in vitro conservation, nursery-scale cultivation, and the restoration of R. carthamoides natural populations. Full article

(This article belongs to the Section Propagation and Seeds)

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18 pages, 2236 KiB

Open AccessArticle

Lethal Effect and Two-Sex Life Table of Tuta absoluta (Meyrick) Treated with Melaleuca alternifolia and Eucalyptus staigeriana Essential Oils

by Brenda C. F. Braga, Dejane S. Alves, Andreísa F. Lima, Júlia A. C. Oliveira, Karolina G. Figueiredo, Vinícius C. Carvalho, Suzan K. V. Bertolucci and Geraldo A. Carvalho

Horticulturae 2025, 11(8), 951; https://doi.org/10.3390/horticulturae11080951 - 12 Aug 2025

Viewed by 187

Abstract

The Tuta absoluta species represents a significant threat to solanaceous crops globally and has developed resistance to conventional synthetic insecticides. This study investigated the insecticidal properties of essential oils (EOs) from Melaleuca alternifolia and Eucalyptus staigeriana against T. absoluta using the age-stage, two-sex [...] Read more.

The Tuta absoluta species represents a significant threat to solanaceous crops globally and has developed resistance to conventional synthetic insecticides. This study investigated the insecticidal properties of essential oils (EOs) from Melaleuca alternifolia and Eucalyptus staigeriana against T. absoluta using the age-stage, two-sex life table methodology. Initially, the EOs of M. alternifolia and E. staigeriana were chemically characterized by gas chromatography (GC) techniques. In this analysis, we identified 19 compounds in M. alternifolia essential oil, with terpinen-4-ol, γ-terpinene, and α-terpinene as the predominant constituents. Eucalyptus staigeriana essential oil contained 25 identified compounds, predominantly limonene, terpinolene, geranial, and neral. Essential oils were dissolved in acetone and applied topically to larval stages. Both treatments significantly reduced pest longevity and adversely affected key demographic parameters. Melaleuca alternifolia treatment resulted in a substantial decrease in the intrinsic rate of population increase, indicating potential for population suppression. These findings support the potential application of M. alternifolia and E. staigeriana EOs as biological control agents against T. absoluta in integrated pest management programs. Full article

(This article belongs to the Special Issue Advanced Strategies for Arthropod Pest Control in Horticultural Systems)

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16 pages, 1331 KiB

Open AccessArticle

Sodium Alginate Composite Coating Inhibited Postharvest Greening and Improved Nutritional Quality of Potato Tubers by Regulating Chlorophyll Biosynthesis

by Chuhan Kang, Xinyu Xia, Dongdong Zhang, Yurong Zhang and Qiong Wu

Horticulturae 2025, 11(8), 950; https://doi.org/10.3390/horticulturae11080950 - 12 Aug 2025

Viewed by 186

Abstract

Potato tuber (Solanum tuberosum L.) was prone to greening and quality deterioration during postharvest storage due to various factors, affecting the regulation of chlorophyll biosynthesis. In the present study, potato tubers were placed at 600 lux and 25 °C after sodium alginate—xanthan [...] Read more.

Potato tuber (Solanum tuberosum L.) was prone to greening and quality deterioration during postharvest storage due to various factors, affecting the regulation of chlorophyll biosynthesis. In the present study, potato tubers were placed at 600 lux and 25 °C after sodium alginate—xanthan gum—glycerin composite coating. During storage, the apparent color changes and a* value of the surface were observed and determined, meanwhile the contents of nutrients, chlorophyll, and its intermediates in photosynthetic metabolism were analyzed. The results showed that after 9 d, compared to the control group, the sodium alginate coating treatment significantly inhibited greening, delayed the decline of appearance quality and nutrients including dry matter, starch, reducing sugar, soluble protein, and ascorbic acid. Furthermore, the sodium alginate coating promoted the contents of 5-aminolevulinic acid (ALA) (1.33 fold), porphobilinogen (PBG) (1.06 fold), and uroporphyrinogen III (Uro III) (1.07 fold), meanwhile, inhibited the production of protoporphyrin IX (Proto IX) (13.86%), Mg-protoporphyrin IX (Mg-Proto IX) (14.15%) and protochlorophyllide (Pchlide) (25.97%), which were key intermediates in the chlorophyll synthesis, indicating that the sodium alginate coating delay the greening by blocking the conversion of Uro III to Proto IX. These results provided valuable insights for the postharvest preservation of potato tuber. Full article

(This article belongs to the Special Issue Postharvest Quality Characteristics and Storage Life of Horticultural Products)

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17 pages, 5705 KiB

Open AccessArticle

Cherry Tomato Bunch and Picking Point Detection for Robotic Harvesting Using an RGB-D Sensor and a StarBL-YOLO Network

by Pengyu Li, Ming Wen, Zhi Zeng and Yibin Tian

Horticulturae 2025, 11(8), 949; https://doi.org/10.3390/horticulturae11080949 - 11 Aug 2025

Viewed by 268

Abstract

For fruit harvesting robots, rapid and accurate detection of fruits and picking points is one of the main challenges for their practical deployment. Several fruits typically grow in clusters or bunches, such as grapes, cherry tomatoes, and blueberries. For such clustered fruits, it [...] Read more.

For fruit harvesting robots, rapid and accurate detection of fruits and picking points is one of the main challenges for their practical deployment. Several fruits typically grow in clusters or bunches, such as grapes, cherry tomatoes, and blueberries. For such clustered fruits, it is desired for them to be picked by bunches instead of individually. This study proposes utilizing a low-cost off-the-shelf RGB-D sensor mounted on the end effector and a lightweight improved YOLOv8-Pose neural network to detect cherry tomato bunches and picking points for robotic harvesting. The problem of occlusion and overlap is alleviated by merging RGB and depth images from the RGB-D sensor. To enhance detection robustness in complex backgrounds and reduce the complexity of the model, the Starblock module from StarNet and the coordinate attention mechanism are incorporated into the YOLOv8-Pose network, termed StarBL-YOLO, to improve the efficiency of feature extraction and reinforce spatial information. Additionally, we replaced the original OKS loss function with the L1 loss function for keypoint loss calculation, which improves the accuracy in picking points localization. The proposed method has been evaluated on a dataset with 843 cherry tomato RGB-D image pairs acquired by a harvesting robot at a commercial greenhouse farm. Experimental results demonstrate that the proposed StarBL-YOLO model achieves a 12% reduction in model parameters compared to the original YOLOv8-Pose while improving detection accuracy for cherry tomato bunches and picking points. Specifically, the model shows significant improvements across all metrics: for computational efficiency, model size (−11.60%) and GFLOPs (−7.23%); for pickable bunch detection, mAP50 (+4.4%) and mAP50-95 (+4.7%); for non-pickable bunch detection, mAP50 (+8.0%) and mAP50-95 (+6.2%); and for picking point detection, mAP50 (+4.3%), mAP50-95 (+4.6%), and RMSE (−23.98%). These results validate that StarBL-YOLO substantially enhances detection accuracy for cherry tomato bunches and picking points while improving computational efficiency, which is valuable for resource-constrained edge-computing deployment for harvesting robots. Full article

(This article belongs to the Special Issue Advanced Automation for Tree Fruit Orchards and Vineyards)

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19 pages, 1833 KiB

Open AccessArticle

Transcriptomics Integrated with Metabolomics Reveals the Accumulation Mechanism of Flavones in Jinsi Huangju

by Yanan Liu, Xinnan Huang, Xinran Chong, Shasha Huang, Changshuai Yu, Hongbin Yu, Yan Wu, Sheng Zeng, Hua Cheng and Guizhen Chen

Horticulturae 2025, 11(8), 948; https://doi.org/10.3390/horticulturae11080948 - 11 Aug 2025

Viewed by 168

Abstract

Chrysanthemum morifolium Ramat. is an important ornamental plant, holding dual economic value as a medicinal and edible plant. Jinsi Huangju is a popular healthy tea drink prepared from the large and elegant shaped flowers of C. morifolium. However, the suboptimal accumulation of [...] Read more.

Chrysanthemum morifolium Ramat. is an important ornamental plant, holding dual economic value as a medicinal and edible plant. Jinsi Huangju is a popular healthy tea drink prepared from the large and elegant shaped flowers of C. morifolium. However, the suboptimal accumulation of bioactive flavonoids during conventional harvest (full bloom stage) limits its commercial potential. To elucidate the molecular mechanisms governing flavonoid biosynthesis in Jinsi Huangju flowers and identify key genetic regulators for metabolic engineering, we performed integrated metabolomic and transcriptomic analyses of flowers at distinct developmental stages using ultra-performance liquid chromatography–tandem mass spectrometry (UPLC-MS/MS) and RNA-seq. Differential metabolites were screened, and candidate genes were validated via transient transformation assays. Among 2146 identified metabolites, flavonoids were the predominant differential compounds, with accumulation patterns being strongly stage dependent. Thirty-eight flavonoid biosynthetic genes and key transcription factors from the MYB, bHLH, and WD40 families exhibited dynamic expression. The CmMYB8a was confirmed as a positive regulator of flavonoid biosynthesis through transient overexpression. This study deciphers the stage-specific flavonoid accumulation in Jinsi Huangju and identifies CmMYB8a as a pivotal regulatory target. Our findings provide genetic resources for breeding high-flavonoid cultivars via molecular design. Full article

(This article belongs to the Topic Genetic Breeding and Biotechnology of Garden Plants)

17 pages, 2507 KiB

Open AccessArticle

Formula Screening and Optimization of Physical and Chemical Properties for Cultivating Flammulina filiformis Using Soybean Straw as Substrate

by Ruixiang Sun, Jiandong Han, Peng Yang, Shude Yang, Hongyan Xie, Jin Li, Chunyan Huang, Qiang Yao, Qinghua Wang, He Li, Xuerong Han and Zhiyuan Gong

Horticulturae 2025, 11(8), 947; https://doi.org/10.3390/horticulturae11080947 - 11 Aug 2025

Viewed by 169

Abstract

Recently, there has been a growing interest in using agricultural and forestry residues to cultivate Flammulina filiformis. However, there is limited research on cultivating F. filiformis with soybean straw as a substrate. This study systematically optimized the cultivation formula for F. filiformis [...] Read more.

Recently, there has been a growing interest in using agricultural and forestry residues to cultivate Flammulina filiformis. However, there is limited research on cultivating F. filiformis with soybean straw as a substrate. This study systematically optimized the cultivation formula for F. filiformis using soybean straw as the raw substrate and explored the effects of the water content, carbon-to-nitrogen ratio (C/N ratio), substrate particle size, and substrate loading on its growth and development. By replacing corncob, wheat bran, and soybean hulls with soybean straw and increasing the proportion of rice bran, the cultivation formula for growing F. filiformis was optimized. We found that the maximum fruiting body yield of 405 g (330 g dry substrate per bottle) and a biological efficiency of 122.73% were achieved using a substrate mixture of 25% soybean straw, 20% corncob, 20% cottonseed hull, 25% rice bran, 8% wheat bran, 1% CaCO₃, and 1% shellfish powder. The yield and biological efficiency of fruiting bodies cultivated on the substrate containing 25% soybean straw did not show significant differences compared to the control group. However, the cultivation formula containing 25% soybean straw yielded F. filiformis with significantly higher levels of amino acids, essential amino acids, and fat. These findings suggest that the 25% soybean straw substrate formulation can serve as a viable alternative to the control formulation for the cultivation of F. filiformis, although variations in the nutritional composition exist. Based on this optimized formula, an optimal biological efficiency can be achieved with a substrate-to-water ratio of 1:1.7, a wet substrate loading amount of 940 g (in a 1250 mL cultivation bottle), and a soybean straw particle size range of 6–8 mm. The optimal C/N ratio for cultivating F. filiformis using soybean straw ranges from 27:1 to 32:1. Additionally, orthogonal experiments revealed that the nitrogen content significantly affected the fruiting body yield, stipe length, and stipe diameter, while the water content mainly affected the pileus diameter, pileus thickness, and number of fruit bodies. Under defined conditions (dry substrate loading volume of 337 g (in a 1250 mL cultivation bottle), a substrate-to-water ratio of 1:1.6, and a C/N ratio of 26:1), the maximum yield and biological efficiency per bottle reached 395 g and 117.21%, respectively. Our findings indicate that the F. filiformis cultivation using soybean straw as the raw substrate exhibits a promising performance and extensive application potential. Full article

(This article belongs to the Special Issue Advances in Propagation and Cultivation of Mushroom)

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21 pages, 1426 KiB

Open AccessArticle

Effects of a Novel Waterlogging-Tolerant Growth-Promoting Pelletizing Agent on the Growth of Brassica napus

by Lingyu Li, Gang Xiao, Hao Jin, Yue Wang, Chunfeng Xie and Zhenqian Zhang

Horticulturae 2025, 11(8), 946; https://doi.org/10.3390/horticulturae11080946 - 11 Aug 2025

Viewed by 231

Abstract

The Yangtze River Basin serves as the primary rapeseed-producing region in China, accounting for over 80% of the national output, yet it is severely impacted by waterlogging, resulting in yield reductions of 17–42.4%. This study investigated the effects of pelleting treatments on growth [...] Read more.

The Yangtze River Basin serves as the primary rapeseed-producing region in China, accounting for over 80% of the national output, yet it is severely impacted by waterlogging, resulting in yield reductions of 17–42.4%. This study investigated the effects of pelleting treatments on growth and waterlogging resistance in Brassica napus varieties Xiangzayou 787 and Fanmingyoutai. Conventional pelleting agents were augmented with waterlogging resistance agents, surfactants, and amino acids as growth-promoting reagents. The results demonstrated that melatonin at

5.0 \times 10^{- 5}

mol/L significantly enhanced rapeseed growth and stress resistance. Specifically, for Xiangzayou 787, root fresh weight increased by 16.9% and stem diameter by 30.6%; for Fanmingyoutai, stem diameter increased by 16.9% and leaf length by 12.3%. The freezing injury index decreased by 90.9% for Xiangzayou 787 and 50% for Fanmingyoutai. The waterlogging injury index was reduced by 43.5% for Xiangzayou 787 and 30.4% for Fanmingyoutai, with stem diameter increasing by 30.6% and 16.5% in the respective varieties. The disease index decreased by 63.2% for Xiangzayou 787 (incidence reduced to 20.5%) and up to 57.1% for Fanmingyoutai (incidence reduced to 23.3%). Under this treatment, soluble protein content in Fanmingyoutai reached 20.37%, representing a 20.37% increase relative to the control. Peroxidase (POD) and superoxide dismutase (SOD) activities exceeded control levels, exhibiting an initial rise followed by a decline; malondialdehyde (MDA) content gradually increased; catalase (CAT) activity and soluble protein content showed an initial increase then decrease. The increase in relative electrical conductivity was reduced by 20.8% for Xiangzayou 787 and 17.3% for Fanmingyoutai. Yield per plant increased by 10.2% for Xiangzayou 787 and 35.6% for Fanmingyoutai. The newly developed pelleting formulation integrates waterlogging resistance agents, surfactants, and amino acids, unlike traditional agents, and proves effective for both hybrid and conventional rapeseed varieties. It enhances waterlogging resistance, promotes growth, improves disease resistance, and elevates seed quality while being cost-effective and simple for production and field application. This approach significantly boosts yield and supports productivity enhancement in southern rice fields, thereby improving rapeseed output and oil supply. Full article

(This article belongs to the Section Biotic and Abiotic Stress)

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17 pages, 3214 KiB

Open AccessArticle

Integrating Transcriptomics and Metabolomics Analyses to Reveal the Potential Molecular Mechanism of Citrus junos Aroma Enhancement by Protected Cultivation

by Fuzhi Ke, Xiu Huang, Lifang Sun, Luoyun Wang, Zhenpeng Nie, Yi Yang and Changjiang Cui

Horticulturae 2025, 11(8), 945; https://doi.org/10.3390/horticulturae11080945 - 11 Aug 2025

Viewed by 264

Abstract

Protected cultivation is a cultivation practice that plays an important role in improving crop quality. Aroma is an important flavour that assesses the quality of yuzu. In this study, C. junos cv. ‘Kitou’ grown in open fields (CJKTF) and plastic greenhouses (CJKTP) were [...] Read more.

Protected cultivation is a cultivation practice that plays an important role in improving crop quality. Aroma is an important flavour that assesses the quality of yuzu. In this study, C. junos cv. ‘Kitou’ grown in open fields (CJKTF) and plastic greenhouses (CJKTP) were selected as the study material. Significant differences in aroma performance between CJKTF and CJKTP were found by the olfactory senses of the members of this research group and an electronic nose, with CJKTP having a stronger aroma. Regarding VOCs, GC-MS analyses revealed 13 VOCs up-regulated and 28 VOCs down-regulated in CJKTP compared to CJKTF. Transcriptome analysis revealed that 515 genes were up-regulated and 720 genes were down-regulated in CJKTP compared to CJKTF. The differential VOCs nerolidol and γ-cadinene, and the differential genes nerolidol synthase 1 (NES1), nerolidol synthase 1-like (NES1-like), and cadinene synthase (DCS), were in the sesquiterpene synthesis pathway and showed significant correlation. NES1, NES1-like, and DCS encode terpene synthases, which may be involved in the biosynthetic pathway of nerolidol and γ-cadinene. In conclusion, the use of plastic greenhouses for cultivation may improve the quality and aroma intensity of yuzu, as well as alter the expression of related genes, compared to field cultivation. These results suggest that protected cultivation is a suitable cultivation practice to enhance the aroma of yuzu. Full article

(This article belongs to the Section Developmental Physiology, Biochemistry, and Molecular Biology)

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14 pages, 1729 KiB

Open AccessArticle

Comparative Genomic Analysis of Wild Cymbidium Species from Fujian Using Whole-Genome Resequencing

by Xinyu Xu, Bihua Chen, Yousry A. El-Kassaby, Juan Zhang, Lanqi Zhang, Sijia Liu, Yu Huang, Junnan Li, Zhiyong Lin, Weiwei Xie, Junjie Wu, Zhiru Lai, Xinzeng Huang, Jianrong Huang, Weijiang Wu and Lihui Shen

Horticulturae 2025, 11(8), 944; https://doi.org/10.3390/horticulturae11080944 - 11 Aug 2025

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Abstract

In this study, we performed whole-genome resequencing (WGS) to investigate genomic variation and functional divergence among four wild Cymbidium species—C. ensifolium, C. sinense, C. kanran, and C. floribundum—collected from Fujian Province, China. A total of 350.58 Gbp of [...] Read more.

In this study, we performed whole-genome resequencing (WGS) to investigate genomic variation and functional divergence among four wild Cymbidium species—C. ensifolium, C. sinense, C. kanran, and C. floribundum—collected from Fujian Province, China. A total of 350.58 Gbp of high-quality sequencing data was obtained from 13 samples, enabling comprehensive identification of SNPs and InDels. Genomic variants were unevenly distributed, with lower variation in gene-rich regions and higher levels in non-coding areas. Circos plots and variant density heatmaps revealed significant regional differences across chromosomes, with longer chromosomes exhibiting greater variant enrichment in 1 Mb windows. C. floribundum harbored the highest number of nonsynonymous SNPs and InDel-associated genes, whereas C. sinense and C. kanran had fewer mutations. KEGG pathway enrichment analysis revealed species-specific functional divergence, particularly in metabolism, stress response, and secondary metabolite biosynthesis. Population structure analysis and principal component analysis (PCA) indicated genetic differentiation among these species Notably, C. kanran exhibited high within-population genetic diversity. These findings provide essential genomic resources for the conservation and functional studies of wild Cymbidium species in subtropical China. Full article

(This article belongs to the Section Genetics, Genomics, Breeding, and Biotechnology (G2B2))

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17 pages, 1214 KiB

Open AccessFeature PaperArticle

Influence of Physiologically Active Substances on the Mineral Composition of Sweet Cherry (Prunus avium L.) Leaves

by Marko Zorica, Tihana Teklić, Marija Špoljarević, Šimun Kolega, Magdalena Zorica, Jelena Ravlić, Tomislav Kos and Miroslav Lisjak

Horticulturae 2025, 11(8), 943; https://doi.org/10.3390/horticulturae11080943 - 11 Aug 2025

Viewed by 296

Abstract

The cultivation of sweet cherry takes place in various climatic zones, where the plant may be exposed to different types of environmental stress during the growing season, which can significantly affect yield and fruit quality. The role of various physiologically active compounds is [...] Read more.

The cultivation of sweet cherry takes place in various climatic zones, where the plant may be exposed to different types of environmental stress during the growing season, which can significantly affect yield and fruit quality. The role of various physiologically active compounds is crucial for plant resistance to stressful environmental conditions. The aim of this study is to determine how the foliar application of different physiologically active substances affects the mineral composition of sweet cherry leaves. Research was performed in 2022 and 2023 at two locations (Ninski Stanovi and Murvica) in Zadar County with the Regina variety. The trials included five foliar treatments (T0—water only, T1—Ca nutritional supplement, T2—biostimulant (Ascophyllum nodosum L.), T3—proline solution, T4—salicylic acid solution). Leaf samples were collected for the analysis of the following macro-elements: total carbon (TC), total nitrogen (TN), calcium (Ca), magnesium (Mg), potassium (K), and phosphorus (P). On average, significantly higher TN content in leaves was found only in T2 (15% higher than T0). Ca, Mg, and K contents in leaf dry matter in all variants were higher by 20–29%, 13–20%, and 12–14%, respectively, compared to the control variant. The significant correlations were found between Ca and Mg, Ca and P, as well as Ca and K contents. This study shows a significant impact of the applied compounds on sweet cherry leaf mineral composition, and considering the year and locality effects, further testing of these treatments in different environments could be suggested. Full article

(This article belongs to the Special Issue Fruit Tree Physiology, Sustainability and Management)

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15 pages, 3904 KiB

Open AccessArticle

MdCDPK24 Encoding Calcium-Dependent Protein Kinase Enhances Apple Resistance to Colletotrichum gloeosporioides

by Jiajun Shi, Yuxin Ma, Dajiang Wang and Feng Wang

Horticulturae 2025, 11(8), 942; https://doi.org/10.3390/horticulturae11080942 - 10 Aug 2025

Viewed by 227

Abstract

Calcium-dependent protein kinases (CDPKs) are unique serine/threonine kinases that play significant roles in response to environmental stresses in plants. In this study, we comprehensively characterized the CDPK gene family in the apple cultivar ‘Hanfu’ at the genome-wide level, and 38 MdCDPKs were identified. [...] Read more.

Calcium-dependent protein kinases (CDPKs) are unique serine/threonine kinases that play significant roles in response to environmental stresses in plants. In this study, we comprehensively characterized the CDPK gene family in the apple cultivar ‘Hanfu’ at the genome-wide level, and 38 MdCDPKs were identified. They were unevenly distributed across 14 chromosomes. Based on phylogenetic analysis, the MdCDPKs were classified into four subfamilies. Conserved domain analysis indicated that MdCDPKs contain the catalytic kinase domain and the Ca²⁺ binding domain. During Colletotrichum gloeosporioides infection, the expression level of MdCDPK24 was significantly upregulated. Subsequently, MdCDPK24 was fused to GFP to generate the MdCDPK24-GFP construct, and confocal microscopy imaging confirmed its cytoplasmic localization in Nicotiana benthamiana leaves. Using agrobacterium-mediated transformation, we generated the overexpression of MdCDPK24 transgenic calli. MdCDPK24-overexpressing calli demonstrated significantly reduced disease severity against C. gloeosporioides infection, indicating its positive role in apple bitter rot resistance. The analysis of the CDPK gene family in the apple cultivar ‘Hanfu’ provides a new insight into the identification of CDPK genes involved in biotic stress. MdCDPK24 represents a promising candidate for genetic manipulation to enhance apple bitter rot resistance. Full article

(This article belongs to the Special Issue Fruit Tree Physiology and Molecular Biology)

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16 pages, 3710 KiB

Open AccessArticle

How Many Acerola (Malpighia emarginata DC.) Fruit Are Required for Reliable Postharvest Quality Assessment?

by João Claudio Vilvert, Cristiane Martins Veloso, Flávio de França Souza and Sérgio Tonetto de Freitas

Horticulturae 2025, 11(8), 941; https://doi.org/10.3390/horticulturae11080941 - 9 Aug 2025

Viewed by 211

Abstract

Acerola (Malpighia emarginata DC.) is a tropical fruit known for its high vitamin C (ascorbic acid) content. This study aimed to determine the optimal sample size (OSS) required to reliably estimate postharvest quality traits in acerola. A total of 50 red-ripe fruit [...] Read more.

Acerola (Malpighia emarginata DC.) is a tropical fruit known for its high vitamin C (ascorbic acid) content. This study aimed to determine the optimal sample size (OSS) required to reliably estimate postharvest quality traits in acerola. A total of 50 red-ripe fruit from four cultivars (BRS Rubra, Cabocla, Costa Rica, and Junko) were evaluated individually for their physical (weight, diameter, length, color, and firmness) and chemical (soluble solids content [SSC], titratable acidity [TA], SSC/TA ratio, and vitamin C) attributes. Bootstrap resampling and nonlinear power models were used to model the relationships between sample sizes and the width of 95% confidence intervals (CI_95%). Three methods were applied to determine the maximum curvature point (MCP): general, perpendicular distance (PD), and linear response plateau (LRP). The PD and LRP methods led to consistent and conservative OSS estimates, which ranged from 12 to 28 fruit depending on the trait and cultivar. A sample size of 20 fruit was identified as a practical and reliable reference. Chemical traits showed greater variability and required larger samples. Cultivar comparisons indicated that ‘BRS Rubra’, ‘Cabocla’, and ‘Costa Rica’ are suitable for fresh consumption, while ‘Junko’ is ideal for vitamin C extraction. These results provide statistical support for experimental planning in acerola postharvest research. Full article

(This article belongs to the Special Issue Postharvest Physiology and Quality Improvement of Fruit Crops)

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10 pages, 2014 KiB

Open AccessArticle

Acrostalagmus luteoalbus as the Novel Causing Agent of Root Rot on Strawberry and In Vitro Screening of Effective Fungicides

by Lei Zhang, Chongyi Fu, Hongling Zhang, Zhengnan Li and Pingping Sun

Horticulturae 2025, 11(8), 940; https://doi.org/10.3390/horticulturae11080940 - 8 Aug 2025

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Abstract

In November 2022, black-root and stem-rot symptoms were observed on the strawberry cultivar ‘Ssanta’ in Hohhot, Inner Mongolia Autonomous Region, China. In order to identify the causing agent of strawberry root rot, and select effective fungicides for controlling this disease, this study was [...] Read more.

In November 2022, black-root and stem-rot symptoms were observed on the strawberry cultivar ‘Ssanta’ in Hohhot, Inner Mongolia Autonomous Region, China. In order to identify the causing agent of strawberry root rot, and select effective fungicides for controlling this disease, this study was carried out. The associated fungi were isolated from diseased strawberry plants, and the isolate that fulfilled Koch’s postulates was further identified based on morphological characteristics, together with the internal transcribed spacer regions and 28S rDNA sequences; then, the inhibitory activities of 11 commercial fungicides on the pathogenic strain were screened based on the mycelium growth method. Results showed that six candidate strains were isolated from diseased strawberry, and only the isolate CMGF-A caused typical root and stem rot on strawberry. The CMGF-A showed typical morphology of Acrostalagmus species, and sequence analyses revealed it as A. luteoalbus. The prochloraz was selected as the primary fungicide for effectively controlling CMGF-A, while tebuconazole, thiophanate-methyl, and difenoconazole·azoxystrobin could be used as alternatives. A. luteoalbus was previously reported to cause potato tuber disease, and red rust of needle mushroom, this work is the first report of A. luteoalbus causing strawberry root rot worldwide. This study provided helpful information for the diagnosis and management of strawberry root rot disease. Full article

(This article belongs to the Special Issue Sustainable Management of Pathogens in Horticultural Crops)

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16 pages, 3152 KiB

Open AccessArticle

Transcriptome Analysis Reveals Potential Mechanism of Regulating Fruit Shape of ‘Laiyang Cili’ Pear with Calyx Excision Treatment

by Huijun Jiao, Yaojun Chang, Qiming Chen, Chaoran Xu, Qiuzhu Guan and Shuwei Wei

Horticulturae 2025, 11(8), 939; https://doi.org/10.3390/horticulturae11080939 - 8 Aug 2025

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Abstract

Fruit shape is an important quality and yield trait of pear, and the fruit shape of ‘Laiyang Cili’ presents a spindle shape which seriously affects its commercial value. Calyx excision treatment could change the fruit shape, while the underlying genes and their regulatory [...] Read more.

Fruit shape is an important quality and yield trait of pear, and the fruit shape of ‘Laiyang Cili’ presents a spindle shape which seriously affects its commercial value. Calyx excision treatment could change the fruit shape, while the underlying genes and their regulatory mechanism remain poorly understood. In this study, we constructed RNA-seq libraries of pear treated with calyx excision to explore underlying regulatory mechanisms. At the early stage of the calyx excision treatment, the numbers of differentially expressed genes (DEGs) between each comparison group were relatively high and gradually decreased along with fruit development. The expression pattern of the DEGs ranked in the top 30 of the six groups had obvious divergence, and DEGs were mainly distributed in the “after calyx excision treatment (0 days)” (AC0d) and AC2d groups. The DEGs were mainly enriched in plant hormone signal transduction and plant defense response. We identified 17 candidate genes related to fruit shape and tested their expression patterns along with fruit development. Among them, nine candidate genes expression trends were consistent with fragments per kilobase of exon model per million mapped fragment (FPKM) values, including MYB62, outer envelope pore protein 62 (OEP62), auxin response factor 3 (ARF3), auxin-responsive protein 50 (SAUR50), protein phosphatase 2C 51 (PP2C 51), major allergen Pyr c 1 (PYRC1), aquaporin TIP1-3 (TIP1-3), transcription factor TGA4 (TGA4) and auxin-responsive protein 17 (IAA17). And then, weighted gene co-expression network analysis (WGCNA) analysis revealed that the OVATE family protein (OFP) and SUN domain-containing protein (SUN) were divided into the MEblue model, which had a positive correlation with calyx excision treatment, and the expression trend of LOC103960706 (OFP8) appeared cohesive with FPKM values. Pbr014104.1 and Pbr016952.1, which were the ortholog genes of LOC103960706, were further identified from the pear genome, and were found to be highly expressed in pear fruit through RT-PCR analysis. Taken together, the key stage determining the development of fruit shape was in the early stage after calyx excision treatment, and fruit shape regulation and development were co-regulated by multiple genes. Full article

(This article belongs to the Special Issue Advances in Developmental Biology in Tree Fruit and Nut Crops—2nd Edition)

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Horticulturae, Volume 11, Issue 8 (August 2025) – 103 articles

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