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19 pages, 3327 KB

Open AccessArticle

Controlling the Bioprinting Efficiency of Alginate–Gelatin by Varying Hydroxyapatite Concentrations to Fabricate Bioinks for Bone Tissue Engineering

by Nikos Koutsomarkos, Varvara Platania, Dimitris Vlassopoulos and Maria Chatzinikolaidou

Polymers 2026, 18(3), 314; https://doi.org/10.3390/polym18030314 - 23 Jan 2026

Abstract

A major objective of this study is to investigate the incorporation of hydroxyapatite nanoparticles (nHA) in a biopolymeric matrix of alginate (Alg) and gelatin (Gel), with particular emphasis understanding how controlled variation in nHA concentration affects rheological, mechanical, printing, and biological performance. Although [...] Read more.

A major objective of this study is to investigate the incorporation of hydroxyapatite nanoparticles (nHA) in a biopolymeric matrix of alginate (Alg) and gelatin (Gel), with particular emphasis understanding how controlled variation in nHA concentration affects rheological, mechanical, printing, and biological performance. Although Alg–Gel blends and nHA-containing hydrogels have been previously explored, a systematic and quantitative correlation between nHA loading, viscoelastic recovery, yield behavior, filament fidelity, and cell viability under optimized bioprinting conditions has not been established. Here, we address this by preparing and evaluating six composite inks (0, 1, 2, 3, 4, and 5% w/v nHA). The parameters of interest included the printing accuracy, the rheological profile, including over 70% viscosity recovery after 10 s in almost all formulations, the elastic modulus, which was over 10 kPa, and the swelling degree. In addition, pre-osteoblastic cells were embedded in these formulations, subsequently bioprinted, and demonstrated viability over 70% after 7 days. The results advance our understanding on the effect of the chemical composition behind the modification of the properties of the composite materials and their applications for biofabrication. This work contributes quantitative insight into how compositional tuning influences the performance of alginate–gelatin–nHA bioinks for extrusion-based bioprinting applications. Full article

(This article belongs to the Special Issue Recent Advances in Natural Biopolymers)

17 pages, 1168 KB

Open AccessReview

Low-Grade Fibromyxoid Sarcoma and Related Subtypes: A Systematic Review and Pooled Analysis of 773 Cases

by Gitte G. J. Krebbekx, Elisabeth A. Kleine, C. Dilara Savci-Heijink, Diederik T. Meijer, Donner, Robert Hemke and Floortje G. M. Verspoor

Cancers 2026, 18(3), 364; https://doi.org/10.3390/cancers18030364 - 23 Jan 2026

Abstract

Background: Low-grade fibromyxoid sarcoma (LGFMS) is a rare malignant fibroblastic tumor that often appears deceptively benign. Accurate diagnosis is challenging due to its variable morphology and low mitotic activity. This systematic review provides a comprehensive overview of LGFMS and its subtypes. Methods: A [...] Read more.

Background: Low-grade fibromyxoid sarcoma (LGFMS) is a rare malignant fibroblastic tumor that often appears deceptively benign. Accurate diagnosis is challenging due to its variable morphology and low mitotic activity. This systematic review provides a comprehensive overview of LGFMS and its subtypes. Methods: A systematic search of PubMed and Embase up to September 2025 identified 273 studies, complemented by four institutional cases from Amsterdam UMC. Individual patient data were pooled to analyze clinical presentation, diagnostic approaches, treatment modalities, and outcomes. Results: In total, 773 patients were included, with a median age of 35 years and equal gender distribution. Tumors were predominantly deep-seated (80%), most commonly located in the thigh or pelvis. MUC4 positivity (96%) and FUS-CREB3L2 fusion (47%) were the most consistent diagnostic markers. Surgery was the mainstay of treatment (98%), with R0 resection achieved in 36% of cases and R1 in 15%. Adjuvant therapies, including chemotherapy and radiotherapy, were rarely used and showed limited efficacy. After a median follow-up of 3.0 years, 19% developed local recurrence and 21% developed metastases. R0 resections were associated with significantly better recurrence-free survival than R1 resection (p < 0.05). Conclusions: LGFMS exhibits indolent histology but potential for late recurrence and metastasis, warranting prolonged radiological follow-up and multicenter studies to evaluate adjuvant strategies. Full article

(This article belongs to the Section Systematic Review or Meta-Analysis in Cancer Research)

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24 pages, 6263 KB

Open AccessReview

Targeting Na_v Channels for Pain Relief: Structural Insights and Therapeutic Opportunities

by Yuzhen Xie, Xiaoshuang Huang, Fangzhou Lu and Jian Huang

Int. J. Mol. Sci. 2026, 27(3), 1180; https://doi.org/10.3390/ijms27031180 - 23 Jan 2026

Abstract

Pain is an unpleasant but essential sensory experience that serves as a protective mechanism, yet it can also manifest maladaptively in a wide range of pathological conditions. Current analgesic strategies rely heavily on opioid medications and non-steroidal anti-inflammatory drugs (NSAIDs); however, concerns regarding [...] Read more.

Pain is an unpleasant but essential sensory experience that serves as a protective mechanism, yet it can also manifest maladaptively in a wide range of pathological conditions. Current analgesic strategies rely heavily on opioid medications and non-steroidal anti-inflammatory drugs (NSAIDs); however, concerns regarding addiction, tolerance, and dose-limiting adverse effects highlight the urgent need for safer and more effective therapeutics. Voltage-gated sodium (Na_v) channels, which govern the initiation and propagation of action potentials, have emerged as promising targets for mechanism-based analgesic development. In particular, the Na_v1.7–Na_v1.9 subtypes have attracted substantial interest owing to their enrichment in the peripheral nervous system—despite broader expression elsewhere—and their central roles in nociception, offering the potential for non-addictive, subtype-selective pain modulation. This review summarizes the physiological roles of these channels in nociception, examines how disease-associated mutations shape pain phenotypes, and highlights recent advances in drug discovery targeting Na_v1.7 and Na_v1.8. The recent FDA approval of VX-548 (suzetrigine), a first-in-class and highly selective Na_v1.8 inhibitor, marks a major milestone that validates peripheral Na_v channels as clinically actionable targets for analgesia. We also discuss the remaining challenges and emerging opportunities in the pursuit of next-generation, mechanism-informed analgesics. Full article

(This article belongs to the Special Issue Role of Ion Channels in Human Health and Diseases)

20 pages, 1146 KB

Open AccessArticle

UAV-Based Forest Fire Early Warning and Intervention Simulation System with High-Accuracy Hybrid AI Model

by Muhammet Sinan Başarslan and Hikmet Canlı

Appl. Sci. 2026, 16(3), 1201; https://doi.org/10.3390/app16031201 - 23 Jan 2026

Abstract

In this study, a hybrid deep learning model that combines the VGG16 and ResNet101V2 architectures is proposed for image-based fire detection. In addition, a balanced drone guidance algorithm is developed to efficiently assign tasks to available UAVs. In the fire detection phase, the [...] Read more.

In this study, a hybrid deep learning model that combines the VGG16 and ResNet101V2 architectures is proposed for image-based fire detection. In addition, a balanced drone guidance algorithm is developed to efficiently assign tasks to available UAVs. In the fire detection phase, the hybrid model created by combining the VGG16 and ResNet101V2 architectures has been optimized with Global Average Pooling and layer merging techniques to increase classification success. The DeepFire dataset was used throughout the training process, achieving an extremely high accuracy rate of 99.72% and 100% precision. After fire detection, a task assignment algorithm was developed to assign existing drones to fire points at minimum cost and with balanced load distribution. This algorithm performs task assignments using the Hungarian (Kuhn–Munkres) method and cost optimization, and is adapted to direct approximately equal numbers of drones to each fire when the number of fires is less than the number of drones. The developed system was tested in a Python-based simulation environment and evaluated using performance metrics such as total intervention time, energy consumption, and task balance. The results demonstrate that the proposed hybrid model provides highly accurate fire detection and that the task assignment system creates balanced and efficient intervention scenarios. Full article

(This article belongs to the Special Issue Advanced Analysis and Technology in Fire Science and Engineering - 2nd Edition)

30 pages, 11053 KB

Open AccessArticle

Investigating the Impact of Education 4.0 and Digital Learning on Students’ Learning Outcomes in Engineering: A Four-Year Multiple-Case Study

by Jonathan Álvarez Ariza and Carola Hernández Hernández

Informatics 2026, 13(2), 18; https://doi.org/10.3390/informatics13020018 - 23 Jan 2026

Abstract

Education 4.0 and digital learning have led to a technology-driven transformation in educational methodologies and the roles of teachers, primarily at Higher Education Institutions (HEIs). From an educational standpoint, the extant literature on Education 4.0 highlights its technological features and benefits; however, there [...] Read more.

Education 4.0 and digital learning have led to a technology-driven transformation in educational methodologies and the roles of teachers, primarily at Higher Education Institutions (HEIs). From an educational standpoint, the extant literature on Education 4.0 highlights its technological features and benefits; however, there is a lack of studies that assess its impact on students’ learning outcomes. Seemingly, Education 4.0 features are taken for granted, as if the technology in itself were enough to guarantee students’ learning, self-efficacy, and engagement. Seeking to address this lack, this study describes the implications of tailoring Education 4.0 tenets and digital learning in an engineering curriculum. Four case studies conducted in the last four years with 119 students are presented, in which technologies such as digital twins, a Modular Production System (MPS), low-cost robotics, 3D printing, generative AI, machine learning, and mobile learning were integrated. With these case studies, an educational methodology with active learning, hands-on activities, and continuous teacher support was designed and deployed to foster cognitive and affective learning outcomes. A mixed-methods study was conducted, utilizing students’ grades, surveys, and semi-structured interviews to assess the approach’s impact. The outcomes suggest that including Education 4.0 tenets and digital learning can enhance discipline-based skills, creativity, self-efficacy, collaboration, and self-directed learning. These results were obtained not only via the technological features but also through the incorporation of reflective teaching that provided several educational resources and oriented the methodology for students’ learning and engagement. The results of this study can help complement the concept of Education 4.0, helping to find a student-centered approach and conceiving a balance between technology, teaching practices, and cognitive and affective learning outcomes. Full article

24 pages, 1906 KB

Open AccessArticle

Towards a Positive Energy District: Energy Efficiency Strategies for an Existing University Campus

by Hamed Mohseni Pahlavan and Natasa Nord

Energies 2026, 19(3), 604; https://doi.org/10.3390/en19030604 (registering DOI) - 23 Jan 2026

Abstract

Developing positive energy districts (PEDs) is a key strategy in the global energy transition to reduce the high energy use and greenhouse gas emissions from the built environment. While the creation of new, energy-efficient urban areas as PEDs is essential, transforming existing districts [...] Read more.

Developing positive energy districts (PEDs) is a key strategy in the global energy transition to reduce the high energy use and greenhouse gas emissions from the built environment. While the creation of new, energy-efficient urban areas as PEDs is essential, transforming existing districts is even more challenging, as they contain buildings of different types, ages, and energy performance levels. This study investigated energy efficiency improvements to facilitate the transition of an existing university campus toward PED operation. The NTNU Gløshaugen campus in Trondheim, Norway, was analyzed using a calibrated multi-building energy model (MBEM) developed using the URBANopt tool. Buildings were clustered into four age-based cohorts to assess the impact of targeted energy conservation measures (ECMs) on different construction periods. In addition, three energy efficiency scenarios were evaluated over the period 2025–2030 to capture the combined effects of new construction and renovation of existing buildings. Results showed that applying envelope improvement ECMs was more effective in older buildings, where lower baseline energy performance allowed for higher relative reductions in energy use. By the end of the simulation period, the specific energy use of the entire campus decreased from 252.2 kWh/m² in 2025 to 161.7 kWh/m² under moderate and 85.9 kWh/m² under deep retrofit conditions. These improvements create more favorable conditions for meeting the remaining energy demand through renewable sources, achieving an overall renewable coverage of 97%, and moving the campus closer to meeting PED targets. Full article

(This article belongs to the Special Issue Advanced Energy Systems in Energy Resilient and Flexible Zero/Positive Energy Buildings, Communities and Districts)

14 pages, 1184 KB

Open AccessArticle

Ibuprofen Improves Wheat Growth Under Salinity by Modulating Hormonal and Antioxidant Status

by Dilara Maslennikova and Oleg Mozgovoj

Plants 2026, 15(3), 360; https://doi.org/10.3390/plants15030360 - 23 Jan 2026

Abstract

Pre-sowing seed treatment (priming) is a strategic tool for programming future crop yield, aimed at improving early plant development and enhancing stress resilience. This study investigated the effects of priming wheat seeds with 100 µM ibuprofen on early ontogeny under optimal conditions and [...] Read more.

Pre-sowing seed treatment (priming) is a strategic tool for programming future crop yield, aimed at improving early plant development and enhancing stress resilience. This study investigated the effects of priming wheat seeds with 100 µM ibuprofen on early ontogeny under optimal conditions and salt stress (100 mM NaCl). An evaluation of germination energy, growth parameters, phytohormone levels (abscisic acid, indolylacetic acid, and cytokinins) and the status of the antioxidant system in 7-day-old seedlings demonstrated that ibuprofen treatment stimulates wheat growth and tolerance, despite its absence of accumulation in plant tissues. Modulation of hormonal balance plays a key role in these protective effects: under optimal conditions, ibuprofen elevates abscisic acid and indolylacetic acid levels, while under salt stress, it prevents excessive abscisic acid accumulation and mitigates the stress-induced decline in indolylacetic acid and cytokinins. Furthermore, ibuprofen promotes a coordinated increase in glutathione, ascorbate, and H₂O₂ levels, concomitant with the activation of key enzymes (glutathione reductase and ascorbate peroxidase), thereby enhancing the plants’ antioxidant potential. Under saline conditions, ibuprofen pretreatment also reduces stress-induced dysregulation of this system. Therefore, ibuprofen acts as a hormetic preconditioning agent that improves seedling vigor and stress tolerance by fine-tuning hormonal signaling and redox metabolism. Full article

(This article belongs to the Special Issue Mitigation Strategies and Tolerance of Plants to Abiotic Stresses—3rd Edition)

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24 pages, 7094 KB

Open AccessArticle

Research on Pilot Workload Identification Based on EEG Time Domain and Frequency Domain

by Weiping Yang, Yixuan Li, Lingbo Liu, Haiqing Si, Haibo Wang, Ting Pan, Yan Zhao and Gen Li

Aerospace 2026, 13(2), 114; https://doi.org/10.3390/aerospace13020114 - 23 Jan 2026

Abstract

Pilot workload is a critical factor influencing flight safety. This study collects both subjective and objective data on pilot workload using the NASA-TLX questionnaire and electroencephalogram acquisition systems during simulated flight tasks. The raw EEG signals are denoised through preprocessing techniques, and relevant [...] Read more.

Pilot workload is a critical factor influencing flight safety. This study collects both subjective and objective data on pilot workload using the NASA-TLX questionnaire and electroencephalogram acquisition systems during simulated flight tasks. The raw EEG signals are denoised through preprocessing techniques, and relevant EEG features are extracted using time-domain and frequency-domain analysis methods. One-way ANOVA is employed to examine the statistical differences in EEG indicators under varying workload levels. A fusion model based on CNN-Bi-LSTM is developed to train and classify the extracted EEG features, enabling accurate identification of pilot workload states. The results demonstrate that the proposed hybrid model achieves a recognition accuracy of 98.2% on the test set, confirming its robustness. Additionally, under increased workload conditions, frequency-domain features outperform time-domain features in discriminative power. The model proposed in this study effectively recognizes pilot workload levels and offers valuable insights for civil aviation safety management and pilot training programs. Full article

(This article belongs to the Special Issue Human Factors and Performance in Aviation Safety)

19 pages, 1235 KB

Open AccessArticle

Enhancing Frost Durability of Cement-Stabilized Silty Clay: Experimental Evaluation and Prediction Model Development

by Yu Zhang, Lingjie Li and Bangyan Hu

Buildings 2026, 16(3), 484; https://doi.org/10.3390/buildings16030484 - 23 Jan 2026

Abstract

Ensuring the long-term performance of infrastructure in cold regions necessitates evaluating the frost durability of subgrade materials. This study comprehensively investigates the mechanical behavior of cement-stabilized silty clay, a common material for subgrade improvement, under freeze–thaw (F–T) cycles. A series of unconfined compressive [...] Read more.

Ensuring the long-term performance of infrastructure in cold regions necessitates evaluating the frost durability of subgrade materials. This study comprehensively investigates the mechanical behavior of cement-stabilized silty clay, a common material for subgrade improvement, under freeze–thaw (F–T) cycles. A series of unconfined compressive strength (UCS) and resilient modulus (MR) tests were conducted to quantify the effects of cement content (3%, 6%, 9%), initial moisture content (OMC − 2% to OMC + 6%), and the number of F–T cycles (0 to 9). The results demonstrate that increasing the cement content significantly enhances the MR, with the most effective improvement observed up to 6%. Specifically, increasing cement from 3% to 6% boosted MR by 11.62% to 26.69%, while a further increase to 9% yielded a smaller gain of 4.59% to 12.60%, indicating an optimal content. Both UCS and MR peak at the optimum moisture content (OMC) and degrade markedly with F–T cycles, with the first cycle causing over 50% of the total MR loss in most cases. Properties tend to stabilize after approximately six cycles. The stabilized soil exhibits superior performance, with its MR being 2.29–2.43 times that of the original soil at OMC after nine F–T cycles. Furthermore, a logarithmic model (R² = 0.87–0.94) effectively captures the attenuation of MR with F–T cycles, while a strong linear relationship (R² = 0.90–0.96) exists between the initial moisture content and the degradation coefficient. An empirical predictive model for UCS, integrating cement content, moisture content, and F–T cycles, is proposed and shows excellent correlation with experimental data (R² > 0.92). Microstructural analysis reveals that the enhancement mechanism is attributed to hydration, cation exchange, and flocculation, which collectively form a stable cementitious network. The findings and proposed models provide critical quantitative insights for optimizing the design of frost-resistant cement-stabilized subgrades, thereby contributing to the enhanced durability and performance of overlying structures in seasonal freeze–thaw environments. Full article

(This article belongs to the Special Issue Foundation Treatment and Building Structural Performance Enhancement)

18 pages, 5643 KB

Open AccessArticle

Chemical Characteristics and Source Identification of PM_2.5 in Industrial Complexes, Korea

by Hyeok Jang, Shin-Young Park, Ji-Eun Moon, Young-Hyun Kim, Joong-Bo Kwon, Jae-Won Choi and Cheol-Min Lee

Toxics 2026, 14(2), 111; https://doi.org/10.3390/toxics14020111 - 23 Jan 2026

Abstract

The composition of air pollutants in industrial complexes differs from that of general urban areas, often containing more hazardous substances that pose significant health risks to both workers and residents nearby. In this study, PM_2.5 and its 29 chemical components (eight ions, [...] Read more.

The composition of air pollutants in industrial complexes differs from that of general urban areas, often containing more hazardous substances that pose significant health risks to both workers and residents nearby. In this study, PM_2.5 and its 29 chemical components (eight ions, two carbon species, and 19 trace elements) were measured and analyzed at five monitoring sites adjacent to the Yeosu and Gwangyang industrial complexes from August 2020 to December 2024. Chemical characterization and source identification were conducted. The average PM_2.5 concentration was 18.63 ± 9.71 μg/m³, with notably higher levels observed during winter and spring. A low correlation (R = 0.56) between elemental carbon (EC) and organic carbon (OC) suggests a dominance of secondary aerosols. The charge balance analysis of [NH₄⁺] with [SO₄²⁻], [NO₃⁻], and [Cl⁻] showed slopes below the 1:1 line, indicating that NH₄⁺ is capable of neutralizing these anions. Positive matrix factorization (PMF) identified eight contributing sources—biomass burning (10.4%), sea salt (11.8%), suspended particles (7.1%), industrial sources (4.6%), Asian dust (5.2%), steel industry (21.8%), secondary nitrate (16.4%), and secondary sulfate (22.7%). These findings provide valuable insights for the development of targeted mitigation strategies and the establishment of effective emission control policies in industrial regions. Full article

(This article belongs to the Section Air Pollution and Health)

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10 pages, 711 KB

Open AccessArticle

Differential Uptake and Release of Female Genital Secretions Components and HPV DNA by Veil, Swab, and Vaginal Tampon

by Ralph-Sydney Mboumba Bouassa, Jonathan Muwonga Tukisadila and Laurent Belec

Diagnostics 2026, 16(3), 380; https://doi.org/10.3390/diagnostics16030380 - 23 Jan 2026

Abstract

Background/Objectives: Self-collection devices are more widely used than ever for detecting sexually transmitted infections and cervical cancer. Despite this, we still lack a clear understanding of how well these tools actually collect and release the necessary molecular samples. This study compared the [...] Read more.

Background/Objectives: Self-collection devices are more widely used than ever for detecting sexually transmitted infections and cervical cancer. Despite this, we still lack a clear understanding of how well these tools actually collect and release the necessary molecular samples. This study compared the in vitro uptake and release performance of commonly used self-sampling devices for total proteins, nucleic acids, and episomal human papillomavirus type 16 (HPV-16) DNA. Methods: An artificial cervicovaginal fluid composed of phosphate-buffered saline supplemented with serum and nucleic acid extracts was serially diluted 2-fold. Each dilution was applied for 5 min to the external surfaces of a vaginal veil (Vaginal Veil Collector V-Veil UP2^TM device), a flocked swab (FLOQSwabs^®), and a commercial vaginal tampon. Non-woven surgical tissue and plastic film served as controls. Total proteins and nucleic acids were quantified by spectrophotometry, and HPV-16 DNA by real-time quantitative PCR. Results: Recovery rates for proteins and nucleic acids were highest for the vaginal veil (81% and 91%), followed by the swab (66% and 70%) and non-woven tissue (44% and 47%). In contrast, the tampon and plastic film performed poorly, releasing less than 30% of proteins and negligible amounts of nucleic acids. Episomal HPV-16 DNA release was highest for the veil (89%), compared with the swab (57%), non-woven tissue (37%), tampon (4%), and plastic film (2%). Conclusions: The vaginal veil demonstrated superior uptake and release of proteins, nucleic acids, and HPV-16 DNA at physiological concentrations. Its non-absorbent structure allows high saturation with efficient release of genital components, including microbial genomes, whereas vaginal tampons retained these components, limiting analytical recovery. Full article

(This article belongs to the Section Clinical Laboratory Medicine)

21 pages, 734 KB

Open AccessReview

Commensal Microbiota and Reproductive Health in Livestock: Mechanisms, Cross-System Crosstalk, and Precision Strategies

by Xiaohan Zhou, Jinping Cao, Guanghang Feng, Yaokun Li, Dewu Liu and Guangbin Liu

Animals 2026, 16(3), 371; https://doi.org/10.3390/ani16030371 - 23 Jan 2026

Abstract

Reproductive performance in livestock and poultry is a core determinant of economic efficiency in the animal industry. While traditional research has primarily focused on genetics, endocrinology, and immune regulation, emerging microbiome studies reveal that commensal microbiota within the gut and reproductive tracts play [...] Read more.

Reproductive performance in livestock and poultry is a core determinant of economic efficiency in the animal industry. While traditional research has primarily focused on genetics, endocrinology, and immune regulation, emerging microbiome studies reveal that commensal microbiota within the gut and reproductive tracts play an underestimated yet pivotal role in host reproductive health. This review systematically synthesizes recent advances regarding the relationship between the microbiome and reproductive functions in major livestock species (cattle, pigs, sheep, and chickens). We first delineate the theoretical basis and mechanisms of the “gut-reproductive axis,” highlighting cross-system communication mediated by microbial metabolites, including short-chain fatty acids (SCFAs), indoles, and bile acids. Subsequently, we provide an in-depth comparative analysis of the microecological features of both female (vagina/uterus) and male (semen/epididymis) reproductive systems, examining their impacts on fertility, sperm quality, and pregnancy outcomes. Furthermore, we explore the molecular and systemic mechanisms governing microbial regulation of reproduction, encompassing the modulation of the hypothalamic-pituitary-gonadal (HPG) axis, the balance of local mucosal immunity and inflammation, and epigenetic regulation. Finally, we address current challenges—such as causal validation and the scarcity of multi-species databases—and propose future directions, including spatial multi-omics, AI-integrated analysis, and microbial intervention strategies. Ultimately, this review aims to offer a theoretical foundation and translational insights for elucidating reproductive regulatory networks and developing microbiome-driven precision strategies to enhance reproductive performance. Full article

(This article belongs to the Section Small Ruminants)

20 pages, 1939 KB

Open AccessArticle

Fiber-Diode Hybrid Laser Welding of IGBT Copper Terminals

by Miaosen Yang, Qiqi Lv, Shengxiang Liu, Qian Fu, Xiangkuan Wu, Yue Kang, Xiaolan Xing, Zhihao Deng, Fuxin Yao and Simeng Chen

Metals 2026, 16(2), 139; https://doi.org/10.3390/met16020139 - 23 Jan 2026

Abstract

The traditional ultrasonic bonding technique for IGBT T2 copper terminals often causes physical damage to ceramic substrates, severely compromising the reliability of power modules. Meanwhile, T2 copper laser welding faces inherent challenges including low laser absorption efficiency and unstable molten pool dynamics. To [...] Read more.

The traditional ultrasonic bonding technique for IGBT T2 copper terminals often causes physical damage to ceramic substrates, severely compromising the reliability of power modules. Meanwhile, T2 copper laser welding faces inherent challenges including low laser absorption efficiency and unstable molten pool dynamics. To address these issues, this study targets the high-quality connection of IGBT T2 copper terminals and proposes a welding solution integrating a Fiber-Diode Hybrid Laser system with galvo-scanning technology. Comparative experiments between galvo-scanning and traditional oscillation methods CNC scanning were conducted under sinusoidal and circular trajectories to explore the regulation mechanism of welding quality. The results demonstrate that CNC scanning lacks precision in thermal input control, resulting in inconsistent welding quality. Galvo-scanning enables precise modulation of laser energy distribution and molten pool behavior, effectively reducing spatter and porosity defects. It also promotes the transition from columnar grains to equiaxed grains, significantly refining the weld microstructure. Under the sinusoidal trajectory with a welding speed of 20 mm/s, the Lap-shear strength of the galvo-scanned joint reaches 277 N/mm², outperforming all CNC-scanned joints. This research proposes a non-contact welding strategy targeted at eliminating the mechanical failure mechanism associated with conventional ultrasonic bonding of ceramic substrates. It establishes the superiority of galvo-scanning for precision welding of high-reflectivity materials and lays a foundation for its potential application in new energy vehicle power modules and microelectronic packaging. Full article

(This article belongs to the Special Issue Advanced Laser Welding and Joining of Metallic Materials)

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17 pages, 607 KB

Open AccessReview

Vascularised Composite Allotransplantation: EmergingApplications in Reconstructive Surgery and Solid Organ Transplantation

by Cian M. Hehir, Michael O’Connor, Iulia Marinescu, Fungai Dengu, Henk P. Giele and Roisin T. Dolan

Medicina 2026, 62(2), 245; https://doi.org/10.3390/medicina62020245 - 23 Jan 2026

Abstract

Vascularised composite allotransplantation (VCA) has an evolving role in the reconstruction of complex functional and aesthetic deficits non-amenable to autologous or implant-based reconstructive modalities. International applications of VCA span upper extremity, face, abdominal wall, uterus, and penile transplantation, with more than 300 procedures [...] Read more.

Vascularised composite allotransplantation (VCA) has an evolving role in the reconstruction of complex functional and aesthetic deficits non-amenable to autologous or implant-based reconstructive modalities. International applications of VCA span upper extremity, face, abdominal wall, uterus, and penile transplantation, with more than 300 procedures performed worldwide. Among these, abdominal wall transplantation has uniquely contributed to the development of the sentinel skin flap (SSF) concept, in which solid organ transplant patients undergo simultaneous transplantation of a solid organ and a donor-derived vascularised skin flap, with the skin component of the SSF being trialled internationally as a means of monitoring for rejection within the solid organ allograft. Despite growing clinical success, VCA continues to face substantial barriers to wider adoption. Acute rejection remains highly prevalent, affecting up to 89% of recipients, with significant morbidity linked to intensive systemic immunosuppression. Challenges are further amplified by the unique immunological heterogeneity of composite grafts, ethical concerns surrounding identity-linked tissues, and the lack of standardised outcomes reporting across VCA subtypes. Advances in machine perfusion technologies and emerging cellular and biomaterial-based immunomodulation strategies show promise in reducing immunosuppression burden and improving graft longevity. This review outlines the current state of VCA, including clinical applications, outcomes, and mechanistic insights from pre-clinical studies, while highlighting key ethical considerations and evolving regulatory frameworks. Future progress will depend on standardised reporting systems, improved donor–recipient matching, better understanding of ischemia–reperfusion injury, and the development of next-generation immunosuppressive/immuno-modulatory therapies. Collectively, these innovations position VCA as a rapidly advancing field with significant potential to redefine reconstructive and transplant surgery. Full article

(This article belongs to the Special Issue Recent Advances in Plastic and Reconstructive Surgery)

19 pages, 883 KB

Open AccessArticle

Smokers, a Way of Harnessing Broadleaf Wood as a Non-Standard Biofuel

by Alessio Ilari, Davide Di Giacinto, Ester Foppa Pedretti, Daniele Duca, Elena Leoni, Thomas Gasperini, Lucia Olivi and Kofi Armah Boakye-Yiadom

Appl. Sci. 2026, 16(3), 1200; https://doi.org/10.3390/app16031200 - 23 Jan 2026

Abstract

Residential barbecuing is becoming increasingly popular worldwide, especially in cities, where it is not only a leisure activity but also an important social and cultural practice. Consequently, the number of grills and smokers in use continues to grow. This study evaluated the environmental [...] Read more.

Residential barbecuing is becoming increasingly popular worldwide, especially in cities, where it is not only a leisure activity but also an important social and cultural practice. Consequently, the number of grills and smokers in use continues to grow. This study evaluated the environmental performance of a household wood-pellet barbecue dual-function smoker/grill using a life cycle assessment (LCA) approach. The functional units selected were per cooking time (1 h) and per unit of energy delivered (1 kWh) at different cooking settings on the smoker. The results show that most of the impacts, including global warming potential (GWP) and resource use, originate from the production of the smoker itself, whereas emissions released during combustion, especially NO_x, are the main contributors to impacts such as acidification and smog formation. The GWP per hour of operation ranged from 0.44 to 0.63 kg CO₂ eq. From an operational perspective, cooking at intermediate temperatures (between 110 and 175 °C) generally leads to lower impacts per hour than very low-temperature smoking. When considering entire meals, meat typically accounts for most of the total impact, with the smoker’s contribution comparatively small. Overall, the study provides a useful reference and shows that both equipment design and food choices play a role in barbecue sustainability. Full article

(This article belongs to the Special Issue Innovative Engineering Technologies for the Agri-Food Sector)

20 pages, 316 KB

Open AccessArticle

Assessing the Critical Thinking and Training Needs of Healthcare Professionals, and Patient Experiences: An Exploratory Cross-Sectional Study in Primary Care of Crete, Greece

by Antonios Christodoulakis, Anna Sergaki, Dimitrios Vavoulas, Izolde Bouloukaki, Michail Zografakis-Sfakianakis, Aristea Mavrogianni, Emmanouil K. Symvoulakis and Ioanna Tsiligianni

Healthcare 2026, 14(3), 294; https://doi.org/10.3390/healthcare14030294 - 23 Jan 2026

Abstract

Background/Objectives: Primary health care (PHC) is the cornerstone of any high-quality healthcare system. For PHC to work well, healthcare professionals need to be skilled in critical thinking, self-reflection, and patient-centered care. However, few studies have explored the potential interplays between these factors. [...] Read more.

Background/Objectives: Primary health care (PHC) is the cornerstone of any high-quality healthcare system. For PHC to work well, healthcare professionals need to be skilled in critical thinking, self-reflection, and patient-centered care. However, few studies have explored the potential interplays between these factors. Therefore, this cross-sectional study evaluated the critical thinking disposition and training needs of PHC professionals, alongside patient experiences and satisfaction with PHC services. Methods: The study involved 54 PHC professionals and 100 patients from sixteen PHC facilities in Crete, Greece. Professionals completed the Critical Thinking Disposition Scale (CTDS) and Training Needs Assessment (TNA) questionnaires, while patients filled out the Quality-of-Life Instrument of Chronic Conditions in Primary Health Care (QUALICOPC) questionnaire. Results: Our findings indicated that PHC professionals exhibited high critical thinking levels (CTDS, mean score of 46.46 ± 4.24). However, TNA scores suggested moderate training needs, particularly in relationships/investigations [median: 0.50 (0, 1.50)], communication/patient-centered [median: 0.30 (0, 1.1)], and flexibility and application of knowledge [median: 0.40 (0, 1.0)]. Nevertheless, no significant correlation was found between CTDS and TNA (ρ = 0.08, p > 0.05). Patients mostly rated their health as poor (40%), and 26% lacked a family physician. Although patients were highly satisfied with communication and patient-centered care (>95% reporting positive experiences), continuity and empowerment had room for improvement. Only 37% felt their GP knew their living conditions, and 26% lacked a personal physician. Patients with chronic conditions reported significantly different experiences. Specifically, patients with chronic conditions had better continuity of care (84% vs. 59%, p = 0.01) and more comprehensive care (70% vs. 43%, p = 0.01) compared to controls. Conclusions: Our findings suggest that targeted training is needed for PHC professionals to address skill gaps. These initial findings could guide the creation of customized professional development initiatives and point to areas where PHC services could be structurally improved. Additional studies, including longitudinal ones, are required to further validate these associations. Full article

19 pages, 1774 KB

Open AccessArticle

Study on Interfacial Shear Bond Behavior Between Ceramsite Foam Concrete and Normal Concrete Under Direct Shear Loading

by Mushan Li, Zhenyun Tang, Zhenbao Li, Chongming Gao and Hua Ma

Buildings 2026, 16(3), 483; https://doi.org/10.3390/buildings16030483 - 23 Jan 2026

Abstract

Ceramsite foam concrete (CFC), recognized for its lightweight, thermal insulation, and eco-friendly properties, is a promising material for composite structures. The interfacial shear bond behavior between CFC and normal concrete (NC) critically governs the structural integrity of CFC-NC systems. This study investigates the [...] Read more.

Ceramsite foam concrete (CFC), recognized for its lightweight, thermal insulation, and eco-friendly properties, is a promising material for composite structures. The interfacial shear bond behavior between CFC and normal concrete (NC) critically governs the structural integrity of CFC-NC systems. This study investigates the interfacial shear bond strength through direct double shear tests on twelve cubic specimens with controlled interface roughness and casting intervals. Quantitative analysis reveals that increased roughness enhances shear strength by up to 28.6~59.5%, while prolonged casting intervals reduce strength by 22.3~34.6%. Notably, excessive roughness shifts failure modes from interfacial debonding to material failure within CFC, where shear bond strength becomes governed by CFC’s compressive strength. A rigid–plastic model is developed to characterize the shear bond behavior of CFC-NC interface and demonstrates 96% accuracy in predicting experimental results. The findings provide useful insights for improving CFC-NC composite design in engineering applications. Full article

(This article belongs to the Section Building Materials, and Repair & Renovation)

13 pages, 3678 KB

Open AccessArticle

Biomechanical Comparison of Different Fixation Methods for Treating Jones Fracture of the Fifth Metatarsal

by Cheng-Min Shih, Yu-Chun Yen, Chun-Hsiang Wang, Yu-Heng Huang, Shun-Ping Wang and Kuo-Chih Su

Bioengineering 2026, 13(2), 135; https://doi.org/10.3390/bioengineering13020135 - 23 Jan 2026

Abstract

Jones fractures are Zone 2 fractures of the fifth metatarsal. Biomechanical comparisons of fixation strategies for Jones fractures remain limited by the lack of standardized, head-to-head evaluations across major fixation methods. The purpose of this study was to perform a standardized biomechanical comparison [...] Read more.

Jones fractures are Zone 2 fractures of the fifth metatarsal. Biomechanical comparisons of fixation strategies for Jones fractures remain limited by the lack of standardized, head-to-head evaluations across major fixation methods. The purpose of this study was to perform a standardized biomechanical comparison of six fixation configurations representing the three primary surgical techniques for Jones fractures and to examine the mechanical factors underlying differences in early construct stability. A synthetic fifth metatarsal model with a simulated Zone 2 fracture was stabilized using lateral plate fixation with different screw configurations, Kirschner wire fixation with or without tension-band wiring, or intramedullary headless screw fixation. All constructs were tested under displacement-controlled cantilever bending, and the force required to reach 1 mm of fracture site displacement was obtained and construct stiffness was calculated. Plate-based fixation demonstrated the highest resistance to bending deformation, followed by intramedullary screw fixation, whereas Kirschner wire-based constructs exhibited the lowest stability. These differences were explained by variations in load-sharing pathways and effective working length among fixation constructs. The addition of tension-band wiring did not result in a measurable improvement in stability compared with Kirschner wire fixation alone, consistent with the dependence of tension-band mechanisms on active muscle loading not represented in the experimental model. These findings provide a unified biomechanical comparison of commonly used fixation constructs for Jones fractures and clarify the mechanical basis for differences in early construct stability. Full article

(This article belongs to the Special Issue Orthopedic and Trauma Biomechanics)

19 pages, 3975 KB

Open AccessArticle

Functional Differences of Glutamine Synthetase Isoenzymes in Wheat Canopy Ammonia Exchange

by Xi Zhang, Junying Chen, Wenjing Song, Siddique Ahmad, Zhiyong Zhang, Huiqiang Li, Xinming Ma, Xiaochun Wang and Yihao Wei

Int. J. Mol. Sci. 2026, 27(3), 1179; https://doi.org/10.3390/ijms27031179 - 23 Jan 2026

Abstract

Canopy ammonia (NH₃) exchange is a major contributor to agricultural NH₃ emissions and is closely linked to nitrogen-use efficiency. Glutamine synthetase (GS) mediates plant NH₃ assimilation, yet the specific roles of different GS isoenzymes in regulating wheat canopy NH [...] Read more.

Canopy ammonia (NH₃) exchange is a major contributor to agricultural NH₃ emissions and is closely linked to nitrogen-use efficiency. Glutamine synthetase (GS) mediates plant NH₃ assimilation, yet the specific roles of different GS isoenzymes in regulating wheat canopy NH₃ exchange remain unclear. This study aimed to clarify the functional differences of wheat TaGS isoenzymes in modulating canopy–atmosphere NH₃ exchange dynamics using two wheat cultivars (Yumai 49-198 and Xinong 509) under two nitrogen application levels (120 and 225 kg N ha⁻¹). Field experiments combined with FTIR-based NH₃ flux measurement, biochemical assays, and molecular analyses were conducted at anthesis and 16, 24, and 30 days after anthesis (DAA). Results showed that the leaf NH₃ compensation point, determined by apoplastic NH₄⁺ concentration, is a key factor influencing canopy NH₃ exchange. Leaf NH₃ sources exhibited distinct temporal specificity: photorespiration and nitrate reduction dominated at anthesis to 16 DAA, whereas nitrogenous compound degradation prevailed at 24–30 DAA. This temporal partitioning was highly coordinated with TaGS isoenzyme expression: TaGS2 was highest in early grain filling, potentially supporting assimilate NH₃ from photorespiration/nitrate reduction, while TaGS1;1 expression increased progressively, aligning with the scavenging of NH₃ from organic nitrogen degradation. These coordinated patterns suggest that the TaGS isoenzymes play differentiated roles in influencing wheat canopy NH₃ exchange. This study thus provides correlative insights that point to potential molecular targets for breeding nitrogen-efficient wheat cultivars and mitigating agricultural NH₃ emissions sustainably. Full article

(This article belongs to the Section Molecular Plant Sciences)

22 pages, 2344 KB

Open AccessArticle

Control of Physically Connected Off-Road Skid-Steering Robotic Vehicles Based on Numerical Simulation and Neural Network Models

by Miša Tomić, Miloš Simonović, Vukašin Pavlović, Milan Banić and Miloš Milošević

Appl. Sci. 2026, 16(3), 1199; https://doi.org/10.3390/app16031199 - 23 Jan 2026

Abstract

The use of robots in various industries has increased significantly in recent years, with mobile robots playing a central role in automation. Their applications range from service robotics and automated material handling to bomb disposal and planetary exploration. A rapidly growing area of [...] Read more.

The use of robots in various industries has increased significantly in recent years, with mobile robots playing a central role in automation. Their applications range from service robotics and automated material handling to bomb disposal and planetary exploration. A rapidly growing area of mobile robotics involves coordinated groups of autonomous robots, commonly referred to as swarms. However, only a limited number of studies have addressed systems in which ropes or wires physically connect robots. Connecting multiple autonomous robotic vehicles with a tensioned wire can form a movable fence, enabling coordinated motion as a single dynamic entity. This paper presents a real-time control approach for the off-road motion of physically connected skid-steering robotic vehicles. A numerical-simulation-driven artificial neural network is employed as a surrogate model to estimate wheel–ground load distribution online, enabling stable steering control and accurate trajectory tracking on rough terrain while accounting for wire-induced coupling effects. Full article

(This article belongs to the Topic Advances in Autonomous Vehicles, Automation, and Robotics)

30 pages, 1035 KB

Open AccessReview

Motor Soft-Start Technology: Intelligent Control, Wide Bandwidth Applications, and Energy Efficiency Optimization

by Peng Li, Li Fang, Pengkun Ji, Shuaiqi Li and Weibo Li

Energies 2026, 19(3), 603; https://doi.org/10.3390/en19030603 (registering DOI) - 23 Jan 2026

Abstract

Direct-starting of industrial motors has problems such as large current impact (five to eight times the rated current), mechanical stress damage, and low energy efficiency. This paper explores the technological innovations in motor soft-start driven by intelligent control and wide-bandgap semiconductors, and constructs [...] Read more.

Direct-starting of industrial motors has problems such as large current impact (five to eight times the rated current), mechanical stress damage, and low energy efficiency. This paper explores the technological innovations in motor soft-start driven by intelligent control and wide-bandgap semiconductors, and constructs a highly reliable and low energy consumption solution. Firstly, based on a material–device–algorithm system framework, a comparative study is conducted on the performance breakthroughs of SiC/GaN in replacing silicon-based devices. Secondly, an intelligent control model is established and a highly reliable system architecture is developed. A comprehensive review of recent literature indicates that SiC devices can reduce switching losses by up to 80%, and intelligent algorithms significantly improve control accuracy. System-level solutions reported in the industry demonstrate the capability to limit current to 1.5–3 times the rated current and achieve substantial carbon emission reductions. These technologies provide key technical support for the intelligent upgrading of industrial motor systems and the dual-carbon goal. In the future, development will continue to evolve in the direction of device miniaturization and other directions. Full article

(This article belongs to the Special Issue Advanced Techniques for Optimization and Energy Management in Smart Grids)

23 pages, 707 KB

Open AccessReview

Plant-Based Nano-Delivery Systems in the Treatment of Inflammatory Disorders

by Catarina R. Silva, Amélia C. F. Vieira, Ana Cláudia Paiva-Santos, Francisco Veiga and Gustavo Costa

Pharmaceutics 2026, 18(2), 150; https://doi.org/10.3390/pharmaceutics18020150 - 23 Jan 2026

Abstract

Inflammation is strongly related to the development of multiple chronic diseases, such as cardiovascular and autoimmune diseases, and is considered a crucial target for new therapeutic approaches, since it significantly impacts public health, contributes to high mortality rates, and decreases the quality of [...] Read more.

Inflammation is strongly related to the development of multiple chronic diseases, such as cardiovascular and autoimmune diseases, and is considered a crucial target for new therapeutic approaches, since it significantly impacts public health, contributes to high mortality rates, and decreases the quality of life. Conventional anti-inflammatory approaches are commonly used, but they present multiple limitations, such as undesirable side effects and low target-specificity. Medicinal plants and their bioactive phytochemical compounds have been studied in recent years and are considered promising alternatives to classical therapies. They are widely recognized for their capacity to modulate inflammatory pathways, regulate inflammatory responses, and consequently reduce inflammation and related symptoms. Although they are considered a good therapeutic alternative, their application in the human body is limited by certain characteristics, such as low solubility, which leads to rapid metabolism and excretion by the organism, significantly reducing bioavailability; for these reasons, the use of medicinal plants remains a biopharmaceutical challenge. Nanotechnology represents a promising tool in this context, since it can improve several characteristics of these compounds. By incorporating plant-derived compounds in nanosystems, considerable advantages, including sustained release, protection from degradation, an increase in the specificity to target tissues, and consequent reduction in toxicity, can be achieved. Thus, nanosystems promote more favorable therapeutic outcomes. This work aims to compile scientific evidence supporting the use of medicinal plants and their bioactive phytochemical compounds, incorporated in nanosystems, in inflammatory disorders. This review enlarges knowledge by integrating both in vitro and in vivo studies involving multiple medicinal plants and bioactive phytochemical compounds, describing their mechanisms of action and the nanosystems employed for drug delivery. In the future, the need for deeper mechanistic studies, the development of targeted and stimuli-responsive systems, and advancement toward clinically translatable, sustainable, and cost-effective plant-based nanotherapies is required. Full article

(This article belongs to the Special Issue Phytocompounds-Based Formulations for Anti-Inflammatory Disorders)

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22 pages, 1257 KB

Open AccessArticle

Chloride-Transporting OsHKT1;1 Splice Variants and Their Expression Profiles Under Salinity Stress in Rice

by Shahin Imran, Shuntaro Ono, Rie Horie, Maki Katsuhara and Tomoaki Horie

Int. J. Mol. Sci. 2026, 27(3), 1178; https://doi.org/10.3390/ijms27031178 - 23 Jan 2026

Abstract

OsHKT1;1, a member of the high-affinity K⁺ transporter (HKT) family, plays a key role in Na⁺ homeostasis and salinity tolerance in rice. In our previous study, multiple potential OsHKT1;1 splicing variants were identified, as well as the full-length (FL) OsHKT1;1 transcript [...] Read more.

OsHKT1;1, a member of the high-affinity K⁺ transporter (HKT) family, plays a key role in Na⁺ homeostasis and salinity tolerance in rice. In our previous study, multiple potential OsHKT1;1 splicing variants were identified, as well as the full-length (FL) OsHKT1;1 transcript from the salt-tolerant rice Pokkali. However, most previous studies focused solely on the full-length protein, leaving the transport functions of splice variants largely unexamined. In this study, we focused on the splice variant OsHKT1;1-V2 and compared its function and gene expression with those of OsHKT1;1-FL. Two-electrode voltage clamp experiments using Xenopus laevis oocytes revealed that the 1st start codon of OsHKT1;1-V2 is functional to exhibit bidirectional currents in bath solutions containing NaCl. Unlike the Na⁺-selective feature of OsHKT1;1-FL, OsHKT1;1-V2 primarily mediated Cl⁻ transport with weak Na⁺ selectivity, which was supported by the higher Cl⁻ accumulation in OsHKT1;1-V2–expressing oocytes. Subcellular localization analyses using oocytes and Arabidopsis mesophyll cells indicated plasma membrane localization of OsHKT1;1-V2, similar to OsHKT1;1-FL. Functional assays using a yeast mutant further indicated that OsHKT1;1-FL, but not OsHKT1;1-V2, mediates Na⁺ uptake. The same OsHKT1;1 variants were identified in the japonica cultivar Nipponbare, and OsHKT1;1-V2 of the cultivar showed Cl⁻ transport properties similar to the one from Pokkali. Quantitative PCR analyses revealed higher abundance of OsHKT1;1-FL transcripts in Nipponbare than in Pokkali with markedly lower OsHKT1;1-V2 levels in Pokkali under salt stress. This study provides a new insight into HKT-mediated ion homeostasis under salinity stress. Full article

(This article belongs to the Special Issue Abiotic Stress Tolerance and Genetic Diversity in Plants, 2nd Edition)

21 pages, 863 KB

Open AccessArticle

Evaluation of Lupin Varieties and Assessment of Adaptability to Neutral-pH Soils Via Recording of Morphological, Agronomical, and Seed Quality Characteristics

by Anna Pitsikoglou, Georgios C. Menexes, Zoi M. Parissi, Maria Irakli, Irini Nianiou-Obeidat, Eleni M. Abraham and Athanasios Mavromatis

Agronomy 2026, 16(3), 289; https://doi.org/10.3390/agronomy16030289 - 23 Jan 2026

Abstract

White lupin (Lupinus albus) is a very important legume crop, having seeds with high protein content but also quantities of antinutritional alkaloids. Regarding cultivation, it is sensitive to neutral or alkaline soil conditions, although it is well adapted to drought conditions. In [...] Read more.

White lupin (Lupinus albus) is a very important legume crop, having seeds with high protein content but also quantities of antinutritional alkaloids. Regarding cultivation, it is sensitive to neutral or alkaline soil conditions, although it is well adapted to drought conditions. In this study, the adaptability of 17 L. albus (14 commercial varieties and 3 advanced lines) genotypes to neutral-pH soils was investigated in relation to morphological, agronomical, and yield attributes. An extended characterization of seed composition for total alkaloids, trypsin inhibitors, phenolics, tannins, total nitrogen, NDF, ADF, and lignin was also performed. Furthermore, a prebreeding program consisting of 140 targeted crosses was initiated to develop new F1 combinations for genotypes with low alkaloid profiles; at the same time, controlled self-fertilization of elite lines was carried out. The results indicated that the morphological response of L. albus to neutral pH was positive and significantly genotypically dependent. Among the varieties tested, ‘Magnus’ and ‘Figaro’ showed low alkaloid and lignin contents. On the other hand, the advanced lines (LKAP, LKML, LKAU) had high antinutritional components, even though they were high-yielding. This research proposes a model of combined evaluation and selection processes for identification of particular genotypes that can perform well in neutral soils and provides the basis for breeding and producing low-alkaloid genotypes for multi-locational exploitation. Full article

(This article belongs to the Special Issue Genetic Identification and Characterisation of Crop Agronomic Traits and Stress Resistance—2nd Edition)

28 pages, 20290 KB

Open AccessArticle

Multi-Technique Characterization of Antonio Basoli’s Picturesque Views of Bologna: An Integrated Approach to 19th-Century Graphic Heritage Conservation

by Monia Vadrucci, Francesca Incoronato, Carlotta Letizia Zanasi, Camilla Roversi Monaco, Massimo Chiari, Claudia Mazzuca, Stefano Lecci, Costanza Cucci, Marcello Picollo and Lorenzo Stefani

Heritage 2026, 9(2), 44; https://doi.org/10.3390/heritage9020044 - 23 Jan 2026

Abstract

Antonio Basoli’s one hundred Picturesque views of the city of Bologna (1824–1836) include 95 preparatory drawings, 16 of which were executed on oil-impregnated lightweight paper now showing advanced degradation. This study aims to investigate the materials and techniques used by Basoli’s workshop and [...] Read more.

Antonio Basoli’s one hundred Picturesque views of the city of Bologna (1824–1836) include 95 preparatory drawings, 16 of which were executed on oil-impregnated lightweight paper now showing advanced degradation. This study aims to investigate the materials and techniques used by Basoli’s workshop and to develop an evidence-based conservation approach for these fragile works. An integrated analytical methodology combining non-invasive hyperspectral imaging (HSI), Fiber Optics Reflectance Spectroscopy (FORS) and Ion Beam Analysis (IBA) with micro-invasive SEM-EDX and FTIR-ATR spectroscopy was applied on five drawings on lightweight impregnated paper to characterize both the paper supports and drawing media. Linseed oil containing lead-based drying agents was suggested to be the impregnating substance, while iron oxide (sanguine) over metalpoint (Cu, Sb, Pb) defined the graphic media. The detection of copper–lead residues suggests that Basoli employed a direct pressure transfer technique similar to James Watt’s copying machine. Conservation treatments resulted in significant pH stabilization (from 5.35 ± 0.20 to 6.45 ± 0.33) and reduced yellowing (ΔE* = 4.9 ± 1.8) while maintaining the paper’s translucency. The results elucidate the innovative practices of Basoli’s workshop and establish a reproducible analytical and conservation methodology applicable to the preservation of nineteenth-century graphic heritage. Full article

(This article belongs to the Special Issue Deterioration and Conservation of Ancient Writing Supports)

17 pages, 3526 KB

Open AccessArticle

Spectral Precision: The Added Value of Dual-Energy CT for Axillary Lymph Node Characterization in Breast Cancer

by Susanna Guerrini, Giulio Bagnacci, Paola Morrone, Cecilia Zampieri, Chiara Esposito, Iacopo Capitoni, Nunzia Di Meglio, Armando Perrella, Francesco Gentili, Alessandro Neri, Donato Casella and Maria Antonietta Mazzei

Cancers 2026, 18(3), 363; https://doi.org/10.3390/cancers18030363 - 23 Jan 2026

Abstract

Background/Objectives: To develop and validate a predictive model that combines morphological features and dual-energy CT (DECT) parameters to non-invasively distinguish metastatic from benign axillary lymph nodes in patients with breast cancer (BC). Methods: In this retrospective study, 117 patients (median age, [...] Read more.

Background/Objectives: To develop and validate a predictive model that combines morphological features and dual-energy CT (DECT) parameters to non-invasively distinguish metastatic from benign axillary lymph nodes in patients with breast cancer (BC). Methods: In this retrospective study, 117 patients (median age, 65 years; 111 women and 6 men) who underwent DECT followed by axillary lymphadenectomy between April 2015 and July 2023, were analyzed. A total of 375 lymph nodes (180 metastatic, 195 benign) were evaluated. Two radiologists recorded morphological criteria (adipose hilum status, cortical appearance, extranodal extension, and short-axis diameter) and placed regions of interest to measure dual-energy parameters: attenuation at 40 and 70 keV, iodine concentration, water concentration and spectral slope. Normalized iodine concentration was calculated using the aorta as reference. Univariate analysis identified variables associated with metastasis. Multivariate logistic regression with cross-validation was used to construct two models: one based solely on morphological features and one integrating water concentration. Results: On univariate testing, all DECT parameters and morphological criteria differed significantly between metastatic and benign nodes (p < 0.01). In multivariate analysis, water concentration emerged as the only independent DECT predictor (odds ratio = 0.97; p = 0.002) alongside cortical abnormality, absence of adipose hilum, extranodal extension and short-axis diameter. The morphologic model achieved an area under the receiver operating characteristic curve (AUC) of 0.871. Increasing water concentration increased the AUC to 0.883 (ΔAUC = 0.012; p = 0.63, not significant), with internal cross-validation confirming stable performance. Conclusions: A model combining standard morphologic criteria with water concentration quantification on DECT accurately differentiates metastatic from benign axillary nodes in BC patients. Although iodine-based metrics remain valuable indicators of perfusion, water concentration offers additional tissue composition information. Future multicenter prospective studies with standardized imaging protocols are warranted to refine parameter thresholds and validate this approach for routine clinical use. Full article

(This article belongs to the Special Issue Enhancing Precision in Cancer Treatment: AI-Driven Innovations in Imaging)

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