2009 March - 32 articles
Capillary electrophoresis (CE) is a high performance separation technique, which has been developed complementary to HPLC. Many separation principles used in HPLC have been transferred to CE successfully, since method development is easy and consumpt...
Recent efforts for the optimization of biotechnological production processes such as for β-lactam antibiotics comprise, amongst others, metabolomics-based methodologies. In metabolic profiling approaches it is aimed at measuring quantitatively time-d...
It is well accepted, that a HPLC-MS/MS assay can outperform immunoassays in therapeutic drug monitoring (TDM) due to its increased accuracy, sensitivity, and precision. However, the occurrence of sudden and unpredictable ion yield attenuation, can ha...
Specific delivery to tumors and efficient cellular uptake of nucleic acids are major challenges for gene-targeted cancer therapies. Tumor-targeted delivery using antibody fragments or immunoliposomes have already been demonstrated to enhance cellular...
Mimetics of the second mitochondria-derived activator of caspases (Smac) increase the sensitivity of tumor cells towards chemotherapeutics in cancer therapy [1]. Our aim was the discovery of naturally derived small molecule Smac-mimetics from the med...
Several plants including medicinal plants and weeds that contaminate food crops contain pyrrolizidine alkaloids (PA). In the case of medicinal plants not used regularly, a low level of PA can be accommodated, but because food is consumed over a long...
HPLC method development is still considered to be one of the crucial bottlenecks that impede productivity in analytical laboratories. Due to the variety of available columns, the proper selection of the stationary phase usually represents the greates...
UPLC (ultraperformance liquid chromatography) has become commercially available in 2004. Like other chromatographic techniques using sub two micron (STM) particles, UPLC is capable of accelerating existing HPLC methods by a factor of 10 to16. However...
Purpose: To create a skin delivery system based on chitosan-tripolyphosphate nanoparticles (cs-tpp np) for acyclovir with enhanced chemical stability and satisfying permeation through skin.
Purpose: Development and physicochemical characterisation of eudermic submicron emulsions (SE) based on natural surfactants (lecithin, sucrose stearate) with different cyclodextrins as co-stabilising agents.
Introduction: The interaction of particulate drug carriers with cells has generally been assessed in stationary microplate assays. These setups fail to reflect the flow conditions in vivo which generate substantial hydrodynamic drag forces [1]. In or...
Nowadays, the various surfactants find wide application in pharmaceutical industry. The nanoparticle preparation process by emulsion techniques essentially requires a surfactant, most commonly Pluronic® F-68 [1]. This non-ionic tenside influences cel...
Histone deacetylase inhibitors (HDACi) have received high interest as anti-cancer agents recently. HDACi may influence tumor growth by an inhibition of angiogenesis as well as by increasing tumor cell immunogenicity [1]. In many clinical trials the e...
Most docking programs are able to generate conformations similar to crystallographically determined protein/ligand complex structures. However, scoring function have problems at distinguishing conformations from the set of docked poses. The task beco...
The maintenance of physiological relevance in cell models while introducing nucleic acids and other molecules is key to effective life science research and the efficient development of novel therapies. The increasing drive toward use of primary cells...
Drug screening in the clinical setting is very often started or even solely performed with a limited selection of enzyme immunoassays in urine samples. As a general rule positive results must be confirmed by more specific and sensitive methods like G...
Understanding the nature of attention seemed intractable until recent develop-ments in neuroimaging and cognitive psychology have allowed for providing specific anatomical and cognitive details about the attention systems of the human brain.
The influence of New Caledonian sandalwood oil on the autonomic nervous system and on the salvia cortisol level, on subjective evaluations on the Profile of Mood States (POMS), and the resulting perception of facial attractiveness were measured on 50...
The blood-brain barrier (BBB) maintains the homeostasis between the central nervous system and the blood circulation [1]. Until now, no standard in vitro model of the BBB is available. In this contribution a novel BBB in vitro model based on primary...
The ABC-transporter ABCB1 (P-glycoprotein) is a drug efflux pump playing an important role in the transport route of drugs through tissue barriers, protection against xenobiotics, and in cancer drug resistance. Due to this, ABCB1 is notorious for lim...
Cell-based research is a booming sector in life science. In vitro assays on cultured cells greatly foster the development of novel therapy concepts for a broad range of diseases, and upcoming techniques such as tissue engineering will soon bring bene...
Introduction: Microplate-based analytical assays represent one of the most commonly used tools for the sensitive and reproducible detection of bioactive molecules. However, despite the widespread use of microplate assays, the degree of optimisation o...
Background: Bladder cancer is one of the most common malignancies. Advances have been made in diagnosis, therapy, and surveillance resulting in increasing survival rates of patients, but the search for more effective therapies is imperative to be con...
To date, the main two classes of clinically used contrast agents for magnetic resonance imaging (MRI) are soluble paramagnetic gadolinium chelates and superparamagnetic iron oxide particles. While iron oxides predominantly increase T2-relaxivity and...
“JAMU” Indonesian traditional medicine preparations have been utilized extensively by Indonesian people. Due to lack of scientific and clinical data, JAMU has not been recommended for formal medication although it has been practiced long before recor...
For the determination of falcarinol [(3R,9Z)-heptadeca-1,9-diene-4,6-diyn-3-ol] in various genotypes of carrots (Daucus carota L.) a new LC–MS method has been developed and validated. The method consists of accelerated solvent extraction (ASE) of lyo...
With advances in separation technology and structure elucidation, the main bottleneck limiting drug development from natural products is the identification of their cellular targets. Although effective therapeutic agents can be developed and successf...
This study assessed the absolute and relative bioavailabilities of dodeca-2E,4E,8E,10E/Z-tetraenoic acid isobutylamides (tetraene), one of the main bioactive constituents in Echinacea, administered as pure compounds or as an Echinacea purpurea root e...
Organic anion transporter polypeptides (OATPs) mediate the transmembrane uptake of endogenous compounds and clinically important drugs in various tissues thereby effecting drug disposition and tissue penetration. OATPs have also been identified in ga...
Resveratrol is a naturally occurring anticancer compound present in grapes and wine that undergoes pronounced metabolism in human intestine and liver. In order to determine whether resveratrol is also biotransformed in human breast carcinoma, metabol...
Red wine polyphenol extracts (RWPE) have been shown to increase nitric oxide (NO) release from EA.hy926 endothelial cells [1]. Trans-resveratrol seems to partly contribute to this effect by inducing endothelial NO-synthase (eNOS) expression [1]. Aim...
The protein tyrosine phosphatase 1B (PTP1B) is an important negative regulator of the insulin receptor. Elchebly M. et al showed that PTP1B knockout mice enhanced insulin sensitivity and were completely healthy and resistant to weight gain when put o...
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