Abstract
Introduction: Microplate-based analytical assays represent one of the most commonly used tools for the sensitive and reproducible detection of bioactive molecules. However, despite the widespread use of microplate assays, the degree of optimisation of the experimental procedures is questionable [1]. Effective mixing is expected to shorten incubation periods, increase reproducibility and sensitivity. To investigate this, the association of cytoadhesive lectins and lectin-modified nanoparticles to epithelial cell monolayers was determined under stationary and mixing conditions.