Next Article in Journal
Discovery of Chemosensitizing Flavonoids from Eriobotrya japonica Using in silico and HPLC-SPE-NMR Techniques
Previous Article in Journal
How to Deal with Ion Suppression – the Foe of HPLC-MS/MS
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Sci. Pharm.
Volume 77
Issue 6
10.3797/scipharm.oephg.21.SL-04
scipharm-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles thumb_up
...
Endorse textsms
...
Comment
Scientia Pharmaceutica is published by MDPI from Volume 84 Issue 3 (2016). Previous articles were published by another publisher in Open Access under a CC-BY (or CC-BY-NC-ND) licence, and they are hosted by MDPI on mdpi.com as a courtesy and upon agreement with Austrian Pharmaceutical Society (Österreichische Pharmazeutische Gesellschaft, ÖPhG).
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Abstract

Targeted Delivery of siRNA to Tumor Cells with Designed Ankyrin Repeat Protein Nanocomplexes

by
J. WINKLER
1,2,* and
U. ZANGEMEISTER-WITTKE
2
1
Department of Medicinal Chemistry, University of Vienna, Althanstraße 14, 1090 Vienna, Austria
2
Department of Pharmacology, University of Bern, Friedbühlstraße 49, 3010 Bern, Switzerland
*
Author to whom correspondence should be addressed.
Sci. Pharm. 2009, 77(6), 171; https://doi.org/10.3797/scipharm.oephg.21.SL-04
Submission received: 16 April 2009 / Accepted: 16 April 2009 / Published: 16 April 2009
Versions Notes

Abstract

Specific delivery to tumors and efficient cellular uptake of nucleic acids are major challenges for gene-targeted cancer therapies. Tumor-targeted delivery using antibody fragments or immunoliposomes have already been demonstrated to enhance cellular uptake of nucleic acids by receptor-mediated endocytosis. Here we report the first use of an epithelial cell adhesion molecule (EpCAM)-specific designed ankyrin repeat protein (DARPin) as a carrier for siRNA. Designed ankyrin repeat proteins are a novel class of non-immunoglobulin binding proteins relying on the modularity of ankyrins. Their short length, high stability, and the lack of intramolecular cysteines facilitate proper folding, result in high-yield expression in E. coli, and allow engineering procedures usually not well tolerated by antibodies. A DARPin binding to EpCAM was derived from a designed protein library using ribosome display.

Share and Cite

MDPI and ACS Style

WINKLER, J.; ZANGEMEISTER-WITTKE, U. Targeted Delivery of siRNA to Tumor Cells with Designed Ankyrin Repeat Protein Nanocomplexes. Sci. Pharm. 2009, 77, 171. https://doi.org/10.3797/scipharm.oephg.21.SL-04

AMA Style

WINKLER J, ZANGEMEISTER-WITTKE U. Targeted Delivery of siRNA to Tumor Cells with Designed Ankyrin Repeat Protein Nanocomplexes. Scientia Pharmaceutica. 2009; 77(Short Lectures (SL)):171. https://doi.org/10.3797/scipharm.oephg.21.SL-04

Chicago/Turabian Style

WINKLER, J., and U. ZANGEMEISTER-WITTKE. 2009. "Targeted Delivery of siRNA to Tumor Cells with Designed Ankyrin Repeat Protein Nanocomplexes" Scientia Pharmaceutica 77, Short Lectures (SL): 171. https://doi.org/10.3797/scipharm.oephg.21.SL-04

APA Style

WINKLER, J., & ZANGEMEISTER-WITTKE, U. (2009). Targeted Delivery of siRNA to Tumor Cells with Designed Ankyrin Repeat Protein Nanocomplexes. Scientia Pharmaceutica, 77(Short Lectures (SL)), 171. https://doi.org/10.3797/scipharm.oephg.21.SL-04

Article Metrics

Back to TopTop