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Scientia Pharmaceutica is published by MDPI from Volume 84 Issue 3 (2016). Articles in this Issue were published by another publisher in Open Access under a CC-BY (or CC-BY-NC-ND) licence. Articles are hosted by MDPI on as a courtesy and upon agreement with Austrian Pharmaceutical Society (Österreichische Pharmazeutische Gesellschaft, ÖPhG).
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Sci. Pharm. 2009, 77(Short Lectures (SL)), 171;

Targeted Delivery of siRNA to Tumor Cells with Designed Ankyrin Repeat Protein Nanocomplexes

Department of Medicinal Chemistry, University of Vienna, Althanstraße 14, 1090 Vienna, Austria
Department of Pharmacology, University of Bern, Friedbühlstraße 49, 3010 Bern, Switzerland
Author to whom correspondence should be addressed.
Received: 16 April 2009 / Accepted: 16 April 2009 / Published: 16 April 2009
PDF [198 KB, uploaded 11 May 2017]


Specific delivery to tumors and efficient cellular uptake of nucleic acids are major challenges for gene-targeted cancer therapies. Tumor-targeted delivery using antibody fragments or immunoliposomes have already been demonstrated to enhance cellular uptake of nucleic acids by receptor-mediated endocytosis. Here we report the first use of an epithelial cell adhesion molecule (EpCAM)-specific designed ankyrin repeat protein (DARPin) as a carrier for siRNA. Designed ankyrin repeat proteins are a novel class of non-immunoglobulin binding proteins relying on the modularity of ankyrins. Their short length, high stability, and the lack of intramolecular cysteines facilitate proper folding, result in high-yield expression in E. coli, and allow engineering procedures usually not well tolerated by antibodies. A DARPin binding to EpCAM was derived from a designed protein library using ribosome display.
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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WINKLER, J.; ZANGEMEISTER-WITTKE, U. Targeted Delivery of siRNA to Tumor Cells with Designed Ankyrin Repeat Protein Nanocomplexes. Sci. Pharm. 2009, 77, 171.

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