Next Article in Journal
Targeted Delivery of siRNA to Tumor Cells with Designed Ankyrin Repeat Protein Nanocomplexes
Previous Article in Journal
HPLC-ESI-MS/MS Assay for Quantitative Metabolic Profiling in Fermentation Broths from β–Lactam Antibiotics Production
Article Menu

Article Versions

Export Article

Scientia Pharmaceutica is published by MDPI from Volume 84 Issue 3 (2015). Articles in this Issue were published by another publisher in Open Access under a CC-BY (or CC-BY-NC-ND) licence. Articles are hosted by MDPI on as a courtesy and upon agreement with Austrian Pharmaceutical Society (Österreichische Pharmazeutische Gesellschaft, ÖPhG).
Open AccessAbstract
Sci. Pharm. 2009, 77(Short Lectures (SL)), 170; (registering DOI)

How to Deal with Ion Suppression – the Foe of HPLC-MS/MS

Division of Mass Spectrometry and Chromatography, Institute of Medical and Chemical Laboratory Diagnostics (ZIMCL), University Hospital Innsbruck, Austria
Author to whom correspondence should be addressed.
Received: 16 April 2009 / Accepted: 16 April 2009 / Published: 16 April 2009
PDF [195 KB, uploaded 11 May 2017]


It is well accepted, that a HPLC-MS/MS assay can outperform immunoassays in therapeutic drug monitoring (TDM) due to its increased accuracy, sensitivity, and precision. However, the occurrence of sudden and unpredictable ion yield attenuation, can hamper assay performance. Such “ion suppression effects” are considered a central analytical problem if using an MS/MS detector in bio-analysis. Since pathological patient specimen compositions or individual co-medications may be major causes for ion yield fluctuations, measures have to be taken to evaluate these interferences prior to transferring an HPLC-MS/MS assay to routine use.
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Share & Cite This Article

MDPI and ACS Style

SEGER, C.; GRIESMACHER, A. How to Deal with Ion Suppression – the Foe of HPLC-MS/MS. Sci. Pharm. 2009, 77, 170.

Show more citation formats Show less citations formats

Article Metrics

Article Access Statistics



[Return to top]
Sci. Pharm. EISSN 2218-0532 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top