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Sci. Pharm.
Volume 77
Issue 6
10.3797/scipharm.oephg.21.SL-07
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Scientia Pharmaceutica is published by MDPI from Volume 84 Issue 3 (2016). Previous articles were published by another publisher in Open Access under a CC-BY (or CC-BY-NC-ND) licence, and they are hosted by MDPI on mdpi.com as a courtesy and upon agreement with Austrian Pharmaceutical Society (Österreichische Pharmazeutische Gesellschaft, ÖPhG).
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Abstract

Speeding Up Pharmaceutical U-HPLC Method Development with an Integrated, Ultrafast Automated Method Scouting Solution

by
H. HERRMANN
1,*,
S. GHIRLANDA
1,
F. STEINER
2 and
R. SWART
3
1
Dionex Corporation, Solothurnerstr. 259, 4600 Olten, Switzerland
2
Dionex Corporation, Dornierstr. 4, 82110 Germering, Germany
3
Dionex Corporation, Abberdaan 114, 1046 AA Amsterdam, The Netherlands
*
Author to whom correspondence should be addressed.
Sci. Pharm. 2009, 77(6), 174; https://doi.org/10.3797/scipharm.oephg.21.SL-07
Submission received: 16 April 2009 / Accepted: 16 April 2009 / Published: 16 April 2009
Versions Notes

Abstract

HPLC method development is still considered to be one of the crucial bottlenecks that impede productivity in analytical laboratories. Due to the variety of available columns, the proper selection of the stationary phase usually represents the greatest challenge. With the progress in U-HPLC which enables generic gradient separations within 5 minutes or less, ultrafast method development techniques become possible. While conventional LC rarely supported more than one generic gradient run per hour, U-HPLC can do a multitude of chromatographic runs in the same amount of time. This allows changing the experimental approach from thoroughly designing a small number of initial experiments towards a more comprehensive screening along any possible combination of column/eluent/temperature. Thanks to the very short run times of U-HPLC, this can be accomplished within the same time frame as the conventional approach. However this screening provides a significantly broader set of results, more information on the influence of the parameters within the design space, and thus more confidence in the robustness of the new method.

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MDPI and ACS Style

HERRMANN, H.; GHIRLANDA, S.; STEINER, F.; SWART, R. Speeding Up Pharmaceutical U-HPLC Method Development with an Integrated, Ultrafast Automated Method Scouting Solution. Sci. Pharm. 2009, 77, 174. https://doi.org/10.3797/scipharm.oephg.21.SL-07

AMA Style

HERRMANN H, GHIRLANDA S, STEINER F, SWART R. Speeding Up Pharmaceutical U-HPLC Method Development with an Integrated, Ultrafast Automated Method Scouting Solution. Scientia Pharmaceutica. 2009; 77(Short Lectures (SL)):174. https://doi.org/10.3797/scipharm.oephg.21.SL-07

Chicago/Turabian Style

HERRMANN, H., S. GHIRLANDA, F. STEINER, and R. SWART. 2009. "Speeding Up Pharmaceutical U-HPLC Method Development with an Integrated, Ultrafast Automated Method Scouting Solution" Scientia Pharmaceutica 77, Short Lectures (SL): 174. https://doi.org/10.3797/scipharm.oephg.21.SL-07

APA Style

HERRMANN, H., GHIRLANDA, S., STEINER, F., & SWART, R. (2009). Speeding Up Pharmaceutical U-HPLC Method Development with an Integrated, Ultrafast Automated Method Scouting Solution. Scientia Pharmaceutica, 77(Short Lectures (SL)), 174. https://doi.org/10.3797/scipharm.oephg.21.SL-07

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