Table of Contents
Genes, Volume 11, Issue 6 (June 2020) – 125 articles
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Cover Story (view full-size image) Making a mistake when trying to generate a new CRISPR mouse model can waste thousands of dollars [...] Read more. Making a mistake when trying to generate a new CRISPR mouse model can waste thousands of dollars and result in months of lost time. Using mouse blastocysts, we describe, step by step, a fast, reliable, and inexpensive method for testing CRISPR components prior to their use in engineering mice. We discovered that a DNA donor sequence complementary to the guide appeared to be incorporated earlier in development than a same-sense donor. We also found that when donors contained silent mutations about every 10 bp between the cut site and desired mutation, increasing the distance between the two no longer reduced successful donor sequence incorporation. View this paper