Next Article in Journal
Genetic Basis of Maize Resistance to Multiple Insect Pests: Integrated Genome-Wide Comparative Mapping and Candidate Gene Prioritization
Next Article in Special Issue
Mass Spectrometry Based-Proteomic Analysis of Anisakis spp.: A Preliminary Study towards a New Diagnostic Tool
Previous Article in Journal
Autosomal Dominantly Inherited GREB1L Variants in Individuals with Profound Sensorineural Hearing Impairment
Previous Article in Special Issue
Immunoreactive Proteins in the Esophageal Gland Cells of Anisakis Simplex Sensu Stricto Detected by MALDI-TOF/TOF Analysis
Open AccessArticle

Leukocyte Nucleolus and Anisakis pegreffii—When Falling Apart Means Falling in Place

Institute for Oceanography and Fisheries, 21000 Split, Croatia
*
Author to whom correspondence should be addressed.
Genes 2020, 11(6), 688; https://doi.org/10.3390/genes11060688
Received: 26 May 2020 / Revised: 19 June 2020 / Accepted: 20 June 2020 / Published: 23 June 2020
(This article belongs to the Special Issue Novel Omics Studies on Anisakid Nematodes)
The view of the nucleolus as a mere ribosomal factory has been recently expanded, highlighting its essential role in immune and stress-related signalling and orchestrating. It has been shown that the nucleolus structure, formed around nucleolus organiser regions (NORs) and attributed Cajal bodies, is prone to disassembly and reassembly correlated to various physiological and pathological stimuli. To evaluate the effect of parasite stimulus on the structure of the leukocyte nucleolus, we exposed rat peripheral blood mononuclear cells (PBMC) to the crude extract of the nematode A. pegreffii (Anisakidae), and compared the observed changes to the effect of control (RPMI-1640 media), immunosuppressive (MPA) and immunostimulant treatment (bacterial lipopolysaccharide (LPS) and viral analogue polyinosinic:polycytidylic acid (poly I:C)) by confocal microscopy. Poly I:C triggered the most accentuated changes such as nucleolar fragmentation and structural unravelling, LPS induced nucleolus thickening reminiscent of cell activation, while MPA induced disassembly of dense fibrillar and granular components. A. pegreffii crude extract triggered nucleolar segregation, expectedly more enhanced in treatment with a higher dose. This is the first evidence that leukocyte nucleoli already undergo structural changes 12 h post-parasitic stimuli, although these are likely to subside after successful cell activation. View Full-Text
Keywords: Anisakis pegreffii; nucleolus; nucleolar stress; peripheral blood mononuclear leukocytes; Sprague Dawley rat Anisakis pegreffii; nucleolus; nucleolar stress; peripheral blood mononuclear leukocytes; Sprague Dawley rat
Show Figures

Figure 1

MDPI and ACS Style

Mladineo, I.; Hrabar, J. Leukocyte Nucleolus and Anisakis pegreffii—When Falling Apart Means Falling in Place. Genes 2020, 11, 688.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop