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Open AccessArticle

Immunoreactive Proteins in the Esophageal Gland Cells of Anisakis Simplex Sensu Stricto Detected by MALDI-TOF/TOF Analysis

1
Department of Biodiversity and Evolutionary Biology, Museo Nacional de Ciencias Naturales, CSIC, calle José Gutiérrez Abascal 2, 28006 Madrid, Spain
2
Departamento de Protección Vegetal, INIA, Ctra La Coruña Km 7′5, 28040 Madrid, Spain
3
Unidad de Proteómica Centro Nacional de Biotecnología, CSIC, calle Darwin 3, Campus de Cantoblanco, 28049 Madrid, Spain
4
Instituto de Ciencia y Tecnología de Alimentos y Nutrición, CSIC, Calle José Antonio Novais, 10, 28040 Madrid, Spain
5
Servicio de Immunología, Hospital Universitario La Paz. Paseo de la Castellana, 261, 28046 Madrid, Spain
*
Authors to whom correspondence should be addressed.
Genes 2020, 11(6), 683; https://doi.org/10.3390/genes11060683
Received: 29 May 2020 / Revised: 18 June 2020 / Accepted: 19 June 2020 / Published: 22 June 2020
(This article belongs to the Special Issue Novel Omics Studies on Anisakid Nematodes)
In plant and animal nematode parasites, proteins derived from esophageal gland cells have been shown to be important in the host-nematodes relationship but little is known about the allergenic potential of these proteins in the genus Anisakis. Taking into account the increase of anisakiasis and allergies related to these nematodes, immunoreactive properties of gland cell proteins were investigated. Two hundred ventricles were manually dissected from L3 stage larvae of Aniskakis simplex s.s. to allow direct protein analysis. Denaturing gel electrophoresis followed by monochromatic silver staining which revealed the presence of differential (enriched) proteins when compared to total nematode extracts. Such comparison was performed by means of 1D and 2D electrophoresis. Pooled antisera from Anisakis spp.-allergic patients were used in western blots revealing the presence of 13 immunoreactive bands in the ventricular extracts in 1D, with 82 spots revealed in 2D. The corresponding protein bands and spots were excised from the silver-stained gel and protein assignation was made by MALDI-TOF/TOF. A total of 13 (including proteoforms) were unambiguously identified. The majority of these proteins are known to be secreted by nematodes into the external environment, of which three are described as being major allergens in other organisms with different phylogenetic origin and one is an Anisakis simplex allergen. View Full-Text
Keywords: Anisakis simplex; allergens; esophageal glands; proteomics; excretion/secretion Anisakis simplex; allergens; esophageal glands; proteomics; excretion/secretion
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Robertson, L.; C. Arcos, S.; Ciordia, S.; Carballeda-Sanguiao, N.; Mena, M.C.; Sánchez-Alonso, I.; Gonzalez-Muñoz, M.; Careche, M.; Navas, A. Immunoreactive Proteins in the Esophageal Gland Cells of Anisakis Simplex Sensu Stricto Detected by MALDI-TOF/TOF Analysis. Genes 2020, 11, 683.

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