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Open AccessArticle

Arabidopsis V-ATPase d2 Subunit Plays a Role in Plant Responses to Oxidative Stress

1
Key Laboratory of Saline-Alkali Vegetation Ecology Restoration, Northeast Forestry University, Ministry of Education, Harbin 150040, China
2
College of Life Sciences, Northeast Forestry University, Harbin 150040, China
3
College of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin 150030, China
4
The State Key Laboratory of Subtropical Silviculture, Zhejiang Agriculture and Forestry University, Lin’An 311300, Zhejiang, China
*
Author to whom correspondence should be addressed.
Genes 2020, 11(6), 701; https://doi.org/10.3390/genes11060701
Received: 26 May 2020 / Revised: 24 June 2020 / Accepted: 24 June 2020 / Published: 25 June 2020
(This article belongs to the Section Plant Genetics and Genomics)
Vacuolar-type H+-ATPase (V-ATPase), a multisubunit proton pump located on the endomembrane, plays an important role in plant growth. The Arabidopsis thaliana V-ATPase d subunit (VHA-d) consists of two isoforms; AtVHA-d1 and AtVHA-d2. In this study, the function of AtVHA-d2 was investigated. Histochemical analysis revealed that the expression of AtVHA-d1 and AtVHA-d2 was generally highly overlapping in multiple tissues at different developmental stages of Arabidopsis. Subcellular localization revealed that AtVHA-d2 was mainly localized to the vacuole. AtVHA-d2 expression was significantly induced by oxidative stress. Analysis of phenotypic and H2O2 content showed that the atvha-d2 mutant was sensitive to oxidative stress. The noninvasive microtest monitoring demonstrated that the net H+ influx in the atvha-d2 roots was weaker than that in the wild-type under normal conditions. However, oxidative stress resulted in the H+ efflux in atvha-d2 roots, which was significantly different from that in the wild-type. RNA-seq combined with qPCR analysis showed that the expression of several members of the plasma membrane H+-ATPase gene (AtAHA) family in atvha-d2 was significantly different from that in the wild-type. Overall, our results indicate that AtVHA-d2 plays a role in Arabidopsis in response to oxidative stress by affecting H+ flux and AtAHA gene expression. View Full-Text
Keywords: V-ATPase d subunit; oxidative stress; sensitive; H+ flux; plasma membrane H+-ATPase; Arabidopsis V-ATPase d subunit; oxidative stress; sensitive; H+ flux; plasma membrane H+-ATPase; Arabidopsis
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Feng, S.; Peng, Y.; Liu, E.; Ma, H.; Qiao, K.; Zhou, A.; Liu, S.; Bu, Y. Arabidopsis V-ATPase d2 Subunit Plays a Role in Plant Responses to Oxidative Stress. Genes 2020, 11, 701.

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