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Nutrients, Volume 17, Issue 22 (November-2 2025) – 133 articles

Cover Story (view full-size image): This review provides an updated synthesis of the evidence on how selected dietary bioactive compounds may contribute to the prevention and modulation of allergic diseases. It examines omega-3 fatty acids, vitamin D, curcumin, ginger constituents, quercetin and EGCG, focusing on their effects on immune regulation and the pathways involved in allergic inflammation. The review integrates mechanistic data with findings from preclinical and clinical studies, highlighting consistent actions on Th2 activity, epithelial integrity, oxidative stress and mast cell responses. Although clinical results remain variable, the evidence supports the relevance of these compounds within nutritional strategies aimed at improving allergic outcomes. View this paper
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21 pages, 1373 KB  
Article
Post-Exercise Nutrition Knowledge and Adherence to Recommendations Among Amateur Endurance Athletes
by Lilla Csanaky, Ágnes Czeglédiné Asztalos, Dorottya Tóth, Éva Polyák and Mária Figler
Nutrients 2025, 17(22), 3629; https://doi.org/10.3390/nu17223629 - 20 Nov 2025
Viewed by 1514
Abstract
Background/Objectives: Optimal post-exercise nutrition is critical for maximizing recovery and subsequent performance. However, athletes often lack knowledge of guidelines, leading to suboptimal practices, particularly inadequate carbohydrate intake for glycogen resynthesis. This study aimed to assess the adherence of Hungarian endurance athletes to [...] Read more.
Background/Objectives: Optimal post-exercise nutrition is critical for maximizing recovery and subsequent performance. However, athletes often lack knowledge of guidelines, leading to suboptimal practices, particularly inadequate carbohydrate intake for glycogen resynthesis. This study aimed to assess the adherence of Hungarian endurance athletes to nutritional recommendations, identifying deficits and guiding the development of effective educational strategies. Methods: A cross-sectional study surveyed 113 amateur Hungarian endurance athletes (mean age 40.04 ± 9.89 years) training ≥ 3 times/week using a self-developed online questionnaire. A ten-item composite measure, the Post-Exercise Nutrition Recommendation Adherence Score (PENRAS, max 10 points), was calculated to assess adherence. Statistical analyses, including ANOVA and regression, were used to explore factors influencing PENRAS and nutritional practices. Results: The overall mean PENRAS was 5.32 ± 1.52, indicating room for improvement. The most pronounced deficit was observed in quantitative knowledge, with only 1.8% of participants correctly identifying the optimal carbohydrate content required for rapid glycogen resynthesis. Concurrently, high protein content (58.4%) was mentioned by a higher percentage than high carbohydrate content (52.2%) as an aspect of post-exercise meal planning. Triathletes had significantly higher PENRAS than runners (6.28 vs. 4.97, p = 0.001). Higher PENRAS was also significantly associated with consultation with a dietitian (p = 0.018). Reliance on professionals positively predicted knowledge, while online sources were a significant negative predictor. Higher PENRAS was associated with better meal planning and earlier post-exercise meal timing. Conclusions: Endurance athletes’ post-exercise nutritional practices are suboptimal. The findings emphasize the need for targeted interventions prioritizing education on carbohydrate intake and redirecting athletes towards evidence-based information to improve adherence and performance outcomes. Full article
(This article belongs to the Special Issue Effects of Exercise and Diet on Health)
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25 pages, 384 KB  
Article
Meatless Momentum: Food-Related Lifestyle Drivers of Plant-Based Meat Alternative Adoption
by Sylwia Żakowska-Biemans
Nutrients 2025, 17(22), 3628; https://doi.org/10.3390/nu17223628 - 20 Nov 2025
Viewed by 667
Abstract
Background/Objectives: Interest in alternatives to animal-derived products has gained momentum, driven by health, environmental, and ethical concerns. However, consumer interest in plant-based meat alternatives (PBMAs) remains highly heterogeneous. This study employs the core dimensions of the Food-Related Lifestyle (FRL) framework for consumer [...] Read more.
Background/Objectives: Interest in alternatives to animal-derived products has gained momentum, driven by health, environmental, and ethical concerns. However, consumer interest in plant-based meat alternatives (PBMAs) remains highly heterogeneous. This study employs the core dimensions of the Food-Related Lifestyle (FRL) framework for consumer segmentation to deepen understanding of PBMA adoption in a meat-centric context such as Poland, and to derive segment-specific recommendations that support the transition toward plant-based diets. Methods: A cross-sectional survey was conducted among a representative sample of Polish adults (n = 1200). Consumer segmentation was performed using a two-step cluster analysis based on the three FRL dimensions—food involvement, innovativeness, and responsibility. Results: Four distinct consumer clusters emerged, differing significantly across all FRL dimensions (p < 0.001). Cluster 1, Traditionalists (18.5%), demonstrated high food involvement but the lowest innovativeness, showing the highest proportion of non-buyers and strong environmental scepticism. Cluster 2, Conscious Food Enthusiasts (24.6%), demonstrated the highest scores across all FRL dimensions, reported the most frequent PBMA purchases, and showed a strong sustainability orientation combined with a pronounced appreciation for sensory quality and eating enjoyment. Cluster 3, Moderates (38.8%) occupied intermediate positions exhibiting moderate PBMA purchasing frequency but expressing concern about food waste. Cluster 4, Careless Food Lovers (18.2%, n = 218), showed high food involvement and innovativeness, but the lowest responsibility, characterised by pronounced environmental scepticism. Conclusions: The study shows that consumer segments with similar levels of food involvement differ in their perceptions, beliefs, and willingness to adopt PBMAs, primarily according to their environmental orientation. These findings highlight that a strong interest in food alone does not translate into acceptance of plant-based alternatives unless accompanied by sustainability-oriented values. Overall, the results offer practical guidance for designing marketing, product development, and policy initiatives tailored to distinct consumer profiles, supporting the transition toward more plant-based dietary patterns. Full article
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14 pages, 492 KB  
Article
Food Reformulation in New Zealand: A Success Story of Reducing the Sodium Content in Bread from 2003 to 2023
by David Monro, Nan Hu and Rachael Mira McLean
Nutrients 2025, 17(22), 3627; https://doi.org/10.3390/nu17223627 - 20 Nov 2025
Viewed by 547
Abstract
Background: The National Heart Foundation of New Zealand (HF) has successfully supported bread companies to reduce the sodium content in leading selling breads over the past 20 years. Bread is the main source of sodium in the New Zealand (NZ) diet and is [...] Read more.
Background: The National Heart Foundation of New Zealand (HF) has successfully supported bread companies to reduce the sodium content in leading selling breads over the past 20 years. Bread is the main source of sodium in the New Zealand (NZ) diet and is considered a low-cost staple food for many New Zealanders. Methods: We highlight some of the critical success factors in developing a food reformulation programme in NZ, using the changes in the packaged loaf bread category as an example. The research considers sodium reduction through three different approaches: (1) matched products, (2) averages of products and (3) sodium contents of the top-selling breads in 2023. Results: The biggest sodium reductions were for white breads, where the mean sodium content dropped from 517 mg/100 g in 2003 to 389 mg/100 g in 2023, representing a 25% reduction. White breads are priced lower than other breads, highlighting the programme’s impact on health equity. The mean sodium content of all breads involved in the study in 2003 was 472 mg/100 g and in 2023 was 384 mg/100 g, representing a 19% reduction overall in the mean sodium content per 100 g. The mean sodium content for the top 20 breads by sales volume in 2023 was 382 mg/100 g, indicating that companies had reduced sodium in leading selling products and the changes had not compromised sales. Conclusions: A key factor for the success of this salt reduction work is the long-standing relationship between a trusted health organisation (HF) and major bread companies. Sodium targets are set in consultation with key food companies and consider any technical and commercial constraints. Government funding has ensured a long-standing programme of work where trust is built with companies, and targets can be revised and monitored. The opportunity from here is to explore where further reductions can be made in the bread category and where these results can continue to drive success in other food categories. Full article
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30 pages, 450 KB  
Review
Magnesium: Health Effects, Deficiency Burden, and Future Public Health Directions
by Marijana Matek Sarić, Tamara Sorić, Željka Juko Kasap, Nataša Lisica Šikić, Mladen Mavar, Jurgita Andruškienė and Ana Sarić
Nutrients 2025, 17(22), 3626; https://doi.org/10.3390/nu17223626 - 20 Nov 2025
Viewed by 3736
Abstract
Magnesium (Mg2+) is the fourth most abundant cation in the human body and a critical cofactor in hundreds of enzymatic reactions that regulate energy metabolism, neuromuscular function, cardiovascular health, bone integrity, immune defense, and psychological well-being. Despite its essential roles, magnesium [...] Read more.
Magnesium (Mg2+) is the fourth most abundant cation in the human body and a critical cofactor in hundreds of enzymatic reactions that regulate energy metabolism, neuromuscular function, cardiovascular health, bone integrity, immune defense, and psychological well-being. Despite its essential roles, magnesium deficiency remains common worldwide, driven by inadequate dietary intake, chronic diseases, medication use, and lifestyle factors. Low magnesium status is associated with hypertension, type 2 diabetes, osteoporosis, migraines, depression, and chronic inflammation, whereas sufficient intake supports cardiometabolic resilience, skeletal strength, neurological stability, and healthy aging. This review synthesizes current evidence on magnesium metabolism, physiological functions, and the health consequences of deficiency, and it summarizes global status with attention to biomarker limitations, widespread suboptimal intake, and key demographic and lifestyle determinants. It also discusses dietary sources, supplementation, and innovative approaches such as food fortification, personalized nutrition, and improved diagnostic strategies. The evidence highlights magnesium as a modifiable factor with potential to lessen the burden of chronic diseases. Recognizing magnesium deficiency as a pressing but underappreciated public health issue, this article underscores the need for integrated strategies to optimize magnesium balance at both individual and population levels. Full article
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16 pages, 273 KB  
Article
Suboptimal Caloric and Micronutrient Intakes in Female Student Athletes Across Several Division 1 Collegiate Sports
by Rachel L. Victor, Morgan M. Nishisaka, Alexandra F. McGrath, Mia K. Gladding, Liel Grosskopf, Hilla Ben-Moshe, Angelos K. Sikalidis, Aleksandra S. Kristo and Scott K. Reaves
Nutrients 2025, 17(22), 3625; https://doi.org/10.3390/nu17223625 - 20 Nov 2025
Viewed by 686
Abstract
Optimal nutrition in athletes can contribute to training adaptations, recovery, performance, and injury prevention in addition to supporting their overall health and well-being. Macronutrient intake and the link to role in athletic performance has been investigated by several studies, while micronutrient intake in [...] Read more.
Optimal nutrition in athletes can contribute to training adaptations, recovery, performance, and injury prevention in addition to supporting their overall health and well-being. Macronutrient intake and the link to role in athletic performance has been investigated by several studies, while micronutrient intake in athletes appears to be understudied. Therefore, the purpose of this study was to assess caloric and selected micronutrient intakes in female collegiate student-athletes across several sports. The participants from seven sports were instructed to complete 3-day food records; dietary intakes were compared to their individual nutritional needs. Results of this study indicated that average intakes of our participants for vitamin C (132.3%), vitamin K (110.5%), and sodium (173%) intakes were significantly above the recommended intakes. However, average intakes for calories (78.0%), calcium (63.1%), magnesium (68.7%), potassium (83.8%), and iron (80.8%) were all significantly below the recommended intakes. More specifically, indoor volleyball and golf athletes exhibited significantly lower vitamin A intake (53.3% and 43.6%, respectively), while iron insufficiency (% of recommendation) was more pronounced in the indoor volleyball (59.8%), golf (65.4%), and tennis (69.9%) teams. Chronic underconsumption of calories and micronutrients can lead to numerous health and athletic performance related consequences. Thus, recognizing and addressing inadequate intakes is imperative to help the student-athletes meet their needs in alignment with dietary guidelines through nutrition education and counseling, as well as dedicated funding and resources towards promoting their overall health, well-being, and athletic as well as academic performance and success. Full article
(This article belongs to the Section Sports Nutrition)
28 pages, 1524 KB  
Systematic Review
The Role of HMB Supplementation in Enhancing the Effects of Resistance Training in Older Adults: A Systematic Review and Meta-Analysis on Muscle Quality, Body Composition, and Physical Function
by Alberto García-Alonso, Juan Luis Sánchez-González, Víctor Navarro-López, Roberto Méndez-Sánchez and Luis Polo-Ferrero
Nutrients 2025, 17(22), 3624; https://doi.org/10.3390/nu17223624 - 20 Nov 2025
Viewed by 2852
Abstract
Background/Objectives: Resistance training (RT) is a key strategy to counteract age-related declines in muscle strength and physical function. β-hydroxy-β-methylbutyrate (HMB) has been proposed as a complementary supplement to enhance these adaptations. However, the additional effects of RT plus HMB (RT+HMB) compared with RT [...] Read more.
Background/Objectives: Resistance training (RT) is a key strategy to counteract age-related declines in muscle strength and physical function. β-hydroxy-β-methylbutyrate (HMB) has been proposed as a complementary supplement to enhance these adaptations. However, the additional effects of RT plus HMB (RT+HMB) compared with RT alone remain unclear. This systematic review and meta-analysis evaluated the effects of RT+HMB versus RT alone on body composition, muscle quality (MQ), and physical function in older adults. Methods: Following PRISMA guidelines (PROSPERO: CRD420251144810), six databases (PubMed, Scopus, Cochrane Library, CINAHL, Web of Science, and ScienceDirect) were searched up to July 2025. Randomized controlled trials comparing RT+HMB with RT alone were included. Methodological quality was assessed with the PEDro scale and risk of bias using Cochrane RoB 2. Pooled standardized mean differences (SMDs) with 95% confidence intervals (CIs) were calculated. Results: Ten trials (n = 596) met inclusion criteria. RT+HMB produced modest and borderline significant improvements in handgrip strength (SMD 0.24; 95% CI 0.00–0.48; p = 0.05) and moderate benefits in Short Physical Performance Battery (SPPB) scores (SMD 0.54; 95% CI 0.12–0.95; p = 0.01). No significant effects were observed for gait speed, appendicular lean mass, MQ, fat mass, or body weight (p > 0.05). Five trials (50%) were rated at high risk of bias, limiting confidence in pooled estimates. Conclusions: HMB supplementation combined with RT may yield modest improvements in functional performance, particularly handgrip strength and overall physical function, without statically significant effects in body composition and MQ. Further high-quality RCTs are warranted to confirm its clinical relevance. Full article
(This article belongs to the Special Issue Nutritional Status in Community-Dwelling Older Adults)
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20 pages, 4785 KB  
Article
Identifying Key Features Associated with Excessive Fructose Intake: A Machine Learning Analysis of a Mexican Cohort
by Guadalupe Gutiérrez-Esparza, Mireya Martínez-García, María del Carmen González Salazar, Luis M. Amezcua-Guerra, Malinalli Brianza-Padilla, Tania Ramírez-delReal and Enrique Hernández-Lemus
Nutrients 2025, 17(22), 3623; https://doi.org/10.3390/nu17223623 - 20 Nov 2025
Viewed by 479
Abstract
Background: Excessive fructose intake has been linked to adverse metabolic outcomes, yet few studies have comprehensively described the clinical, behavioral, and nutritional patterns associated with different intake levels using machine learning. Methods: In this study, unsupervised and supervised algorithms were applied to a [...] Read more.
Background: Excessive fructose intake has been linked to adverse metabolic outcomes, yet few studies have comprehensively described the clinical, behavioral, and nutritional patterns associated with different intake levels using machine learning. Methods: In this study, unsupervised and supervised algorithms were applied to a healthy Mexican cohort to examine features related to high fructose consumption, defined as intake above 25 g per day. Results: K-Means clustering identified three distinct profiles, with one subgroup showing less favorable anthropometric, biochemical, and behavioral characteristics. Supervised models, including Extreme Gradient Boosting, Random Forest, and Histogram-based Gradient Boosting, distinguished fructose intake levels with balanced accuracies around 80% and AUC up to 88.1%. Shapley Additive Explanations (SHAPs)-based interpretation highlighted body mass index, triglycerides, sleep duration, alcohol consumption, and anxiety indicators as features most consistently associated with high intake. Conclusions: These findings emphasize the multifactorial nature of fructose consumption and illustrate the utility of machine learning for uncovering dietary and metabolic patterns that warrant further investigation and may guide future nutrition-focused strategies. Full article
(This article belongs to the Special Issue Sugar, Sweeteners Intake and Metabolic Health)
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13 pages, 4734 KB  
Article
Development of the Breastfed Infant Oral Microbiome Is Associated with Concentrations and Intakes of Human Milk Oligosaccharides
by Roaa A. Arishi, Ali S. Cheema, Jacki L. McEachran, Zoya Gridneva, Annalee Furst, Ana Roman, Lars Bode, Ching Tat Lai, Matthew S. Payne, Donna T. Geddes and Lisa F. Stinson
Nutrients 2025, 17(22), 3622; https://doi.org/10.3390/nu17223622 - 20 Nov 2025
Viewed by 701
Abstract
Background/Objectives: Human milk oligosaccharides (HMOs) are bioactive carbohydrates abundant in human milk that shape the infant gut microbiome, yet their influence on the oral microbiome remains poorly understood. This study investigated associations between HMO concentrations and infant HMO intakes and the composition [...] Read more.
Background/Objectives: Human milk oligosaccharides (HMOs) are bioactive carbohydrates abundant in human milk that shape the infant gut microbiome, yet their influence on the oral microbiome remains poorly understood. This study investigated associations between HMO concentrations and infant HMO intakes and the composition of the oral microbiome in predominantly (n = 2) and exclusively (n = 54) breastfed infants. Methods: We profiled infant oral samples collected at 2 months of age using full-length 16S rRNA gene sequencing, alongside paired milk HMO analyses from 56 mother–infant dyads from the Western Australian BLOSOM cohort. Daily HMO intakes were calculated using 24 h milk intake data. Results: Concentrations of human milk 3FL and LNFPIII were negatively associated with infant oral Shannon diversity (p = 0.027) and richness (p = 0.037), respectively. LNFPII concentration and daily intake were linked to increased abundance of Neisseria subflava (both p = 0.025), while daily intakes of DFLNT and DFLNH were positively associated with Streptococcus parasanguinis (p = 0.028 and p = 0.040). Notably, the associations observed for daily HMO intakes were modest in effect size. Conclusions: These findings demonstrate a statistically significant but biologically limited effect of HMOs on the development of the infant oral microbiome. By clarifying how specific components of human milk shape early microbial development, this work provides mechanistic insights relevant to nutritional and supportive interventions that promote breastfeeding success. Full article
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15 pages, 438 KB  
Article
Design and Validation of the Index of Adherence to the Dietary Guidelines for Chile 2022 (GABAS-Index 17)
by Catalina Ramírez-Contreras, Jaime Crisosto-Alarcón, Solange Parra-Soto, Jorge Burdiles-Aguirre, Gianella Liabeuf and Lautaro Briones-Suárez
Nutrients 2025, 17(22), 3621; https://doi.org/10.3390/nu17223621 - 20 Nov 2025
Viewed by 438
Abstract
Background/Objectives: Adherence to national dietary guidelines is essential for promoting healthy eating and preventing chronic diseases. In Chile, the 2022 update introduced new evidence-based recommendations, but no validated tool is currently available to assess adherence. The aim of this study was to [...] Read more.
Background/Objectives: Adherence to national dietary guidelines is essential for promoting healthy eating and preventing chronic diseases. In Chile, the 2022 update introduced new evidence-based recommendations, but no validated tool is currently available to assess adherence. The aim of this study was to develop and validate a tool to assess adherence to the updated Chilean dietary guidelines. Methods: For this purpose, five expert judges evaluated the content validity using Aiken’s V (V ≥ 0.80). Reliability was assessed through a 21-day test–retest in 30 participants (≥18 years, mean age 38.9 years; 63.3% women) using the Intraclass Correlation Coefficient (ICC(3,1)), a two-way mixed-effects model to assess the absolute agreement of individual measurements, Standard Error of Measurement (SEM), and Minimal Detectable Change (MDC95) at the 95% confidence level. Internal consistency was assessed in 152 participants (≥18 years) examined via McDonald’s ω, and construct validity through confirmatory factor analysis (CFA) using the WLSMV estimator. Results: The GABAS-Index 17 showed high content validity (Aiken’s V = 0.93–1.00), good internal consistency (ω = 0.64–0.71), and accurate reliability (ICC = 0.905; SEM < 1; MDC95 = 2.1). Confirmatory factor analysis supported the proposed four-dimensional structure (CFI = 1.00; TLI = 1.02; RMSEA = 0.00), confirming strong factorial validity and internal coherence. Conclusions: These findings support the GABAS-Index 17 as an adequate and reliable tool for assessing adherence to the updated Chilean dietary guidelines. Although some psychometric aspects, such as the factorial structure, could be improved, the instrument performs well for its intended purpose of providing an overall adherence score. Its use can facilitate monitoring dietary patterns, support nutrition research, and inform public health strategies to improve diet quality in the Chilean population. Full article
(This article belongs to the Special Issue Dietary Patterns and Data Analysis Methods)
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27 pages, 1256 KB  
Review
Migraine and Alcohol—Is It Really That Harmful?
by Anna Zduńska, Joanna Cegielska, Sebastian Zduński and Izabela Domitrz
Nutrients 2025, 17(22), 3620; https://doi.org/10.3390/nu17223620 - 20 Nov 2025
Viewed by 1809
Abstract
Alcohol is a widely consumed beverage worldwide, and headaches, including migraine, tension-type headache (TTH), and other primary headaches, are common in the general population. Although epidemiological studies have shown a correlation between alcohol consumption and headaches, the specific pathophysiological mechanism of this headache [...] Read more.
Alcohol is a widely consumed beverage worldwide, and headaches, including migraine, tension-type headache (TTH), and other primary headaches, are common in the general population. Although epidemiological studies have shown a correlation between alcohol consumption and headaches, the specific pathophysiological mechanism of this headache remains unknown. We reviewed articles deemed relevant to the issue of alcohol as a trigger for various headaches, those that discussed alcohol consumption in these patient groups, and those that addressed the pathophysiological and clinical aspects of alcohol and headache. The review concluded that alcohol affects both migraine and non-migraine headaches. Alcohol-induced headache, classified as a secondary headache, is a throbbing, bilateral headache that is exacerbated by physical activity and is precipitated by alcohol consumption. TTH can be precipitated by alcohol consumption, and patients with TTH have more alcohol-related problems than those with migraine. Cluster headaches (CH) are often triggered by alcohol, but surprisingly, many CH patients consume alcohol, even during attacks. The relationship between alcohol and migraine is complex. Numerous components of alcoholic beverages can influence pain triggering and are responsible for migraine attacks. Red wine is one of the most frequently cited triggers for migraine attacks, a finding not always confirmed by the few prospective studies. However, there is no safe dose of alcohol, and therefore avoidance should be recommended. Full article
(This article belongs to the Section Nutrition and Public Health)
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33 pages, 1064 KB  
Review
A Systematic Review of Palmitate-Mediated Insulin Resistance in C2C12 Myotubes
by John M. Zimmerman, Alexa J. Klein, Kipton B. Travis and Roger A. Vaughan
Nutrients 2025, 17(22), 3619; https://doi.org/10.3390/nu17223619 - 20 Nov 2025
Viewed by 767
Abstract
Background/Objectives: Skeletal muscle plays a pivotal role in whole-body glucose metabolism and is a major target in the pathogenesis and treatment of insulin resistance and type 2 diabetes. The C2C12 myotube cell line is one of the most used in vitro models to [...] Read more.
Background/Objectives: Skeletal muscle plays a pivotal role in whole-body glucose metabolism and is a major target in the pathogenesis and treatment of insulin resistance and type 2 diabetes. The C2C12 myotube cell line is one of the most used in vitro models to investigate mechanisms of insulin resistance. This systematic review (1) summarizes the most common experimental conditions including palmitate concentrations and treatment durations used to induce insulin resistance in C2C12 myotubes; (2) characterizes outcomes related to insulin resistance; and (3) discusses strengths and limitations associated with this model. Methods: A systematic search of PubMed and Scopus was conducted using terms “C2C12 AND palmitate AND insulin resistance” and related variations. A total of 191 articles met inclusion criteria. Results: The most frequently used palmitate concentrations were 0.25 mM, 0.5 mM, and 0.75 mM for at least 16 h, which consistently led to decreased insulin-stimulated pAkt expression, GLUT4 abundance, and insulin-stimulated glucose uptake. Conclusions: The high volume and consistency of primary findings is a key strength of this article which demonstrated reduced insulin signaling across various culture conditions, treatment durations, and insulin co-stimulation protocols. Full article
(This article belongs to the Section Nutrition and Diabetes)
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15 pages, 1581 KB  
Perspective
Future Directions in Choline: From Neurodevelopment to Cardiometabolic Health
by Evan M. Paules, Hannah G. Petry, Jessica K. Sprinkles and Isis Trujillo-Gonzalez
Nutrients 2025, 17(22), 3618; https://doi.org/10.3390/nu17223618 - 20 Nov 2025
Viewed by 1521
Abstract
Although choline was established as an essential nutrient over three decades ago, critical questions remain about how choline regulates brain, liver, and cardiometabolic health across the lifespan. This Perspective summarizes emerging insights presented at the Future Directions in Choline Symposium: A Tribute to [...] Read more.
Although choline was established as an essential nutrient over three decades ago, critical questions remain about how choline regulates brain, liver, and cardiometabolic health across the lifespan. This Perspective summarizes emerging insights presented at the Future Directions in Choline Symposium: A Tribute to Steven H. Zeisel, which outlined three converging areas of research: (i) choline-dependent mechanisms in neurodevelopment and cognition, (ii) the link between choline metabolism and obesity, and (iii) the role of trimethylamine N-oxide (TMAO) in cardiovascular disease. Evidence from clinical and preclinical studies confirms that maternal choline intake is critical for neurogenesis, cognition, and visual system development, and that higher choline availability buffers the fetal brain against environmental and psychosocial stressors. Choline supplementation improves cognitive outcomes in fetal alcohol spectrum disorder and mitigates neurodegenerative pathology in Alzheimer’s models. In cardiometabolic health, recent data challenge the interpretation of TMAO as a causal toxin, positioning it instead as a marker of renal function. Moving forward, the field must develop validated biomarkers of choline adequacy in free-living populations, harmonize intervention protocols, and define context-specific requirements across obesity, pregnancy, and glucagon-like peptide-1 (GLP-1)-based therapy use. These efforts will refine dietary recommendations and solidify choline’s role in lifelong brain and metabolic health. Full article
(This article belongs to the Section Micronutrients and Human Health)
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19 pages, 760 KB  
Review
The Mediterranean Diet in Primary and Secondary Prevention of Coronary Heart Disease: Evidence and Mechanisms
by Ewelina Młynarska, Julia Kurcińska, Szymon Kurciński, Weronika Marcinkowska, Klaudia Leszto, Paulina Jakubowska, Jacek Rysz and Beata Franczyk
Nutrients 2025, 17(22), 3617; https://doi.org/10.3390/nu17223617 - 20 Nov 2025
Cited by 1 | Viewed by 2192
Abstract
Cardiovascular disease is a major cause of mortality, and its global reach places it as an object of interest for many researchers worldwide. Steps are being actively taken to combat this disease. Current trends focus on non-invasive prevention, specifically dietary interventions of a [...] Read more.
Cardiovascular disease is a major cause of mortality, and its global reach places it as an object of interest for many researchers worldwide. Steps are being actively taken to combat this disease. Current trends focus on non-invasive prevention, specifically dietary interventions of a relatively easily accessible, modifiable therapeutic option. The most promising seems to be the MD, used in both primary and secondary prevention, as reflected in current research. The bioactive components and mechanisms of this dietary pattern confirm its validity. For now, it is at the forefront of other diets available but needs to be improved in terms of precise guidelines for its application to the individual patient. Full article
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23 pages, 4946 KB  
Article
In Vitro Insights into the Antifungal, Prebiotic, and Cytotoxic Potential of Tomato Plant Waste
by Simona Marcu Spinu, Mihaela Dragoi Cudalbeanu, Carmen Laura Cimpeanu, Nikola Major, Elwira Sieniawska, Krzysztof Kamil Wojtanowski, Ionela Avram, Diana Pelinescu, Alina Ortan and Narcisa Elena Babeanu
Nutrients 2025, 17(22), 3616; https://doi.org/10.3390/nu17223616 - 20 Nov 2025
Viewed by 414
Abstract
Background/Objectives: This study aims to screen the extracts of tomato plant waste (aerial parts—mixture of leaves, stems, and bunches resulting from tomato crop maintenance, and axillary shoots—resulting from pruning practices) and evaluate their antifungal, prebiotic, and cytotoxic effects. Methods: A phytochemical profiling was [...] Read more.
Background/Objectives: This study aims to screen the extracts of tomato plant waste (aerial parts—mixture of leaves, stems, and bunches resulting from tomato crop maintenance, and axillary shoots—resulting from pruning practices) and evaluate their antifungal, prebiotic, and cytotoxic effects. Methods: A phytochemical profiling was performed to analyze volatile and semi-volatile compounds by GC-MS, functional groups by FTIR, soluble sugars by HPLC-RI, and glycoalkaloids by LC-MS/MS. Tomato plant waste extracts were further tested in vitro, and their biological effects were assessed with probiotic microorganisms (Enterococcus faecium ATCC 19434, Enterococcus faecium VL43, Lactobacillus plantarum ATCC 8014, and Lactobacillus plantarum GM3) to determine their prebiotic-like properties, particularly after demonstrating strong antifungal activity against several Candida species, such as Candida albicans ATCC 10231, Candida parapsilosis ATCC 22019, Candida glabrata ATCC 64677, and Candida auris 6328. The extracts were also evaluated for the cytotoxic effect against HEP-G2, HeLa, and HT-29 cell lines, while cytotoxicity assays confirmed no significant effects on the normal HEK-293 cell line compared to the control. Results: The in vitro antimicrobial activity and prebiotic-like substrate assay proved the difference between extract effects against Candida species (C. glabrata—MIC 125 µg/mL) and, respectively, the influence on Lactobacillus strains growth (up to a 1.6-fold increase in OD600). Furthermore, they exhibited selective cytotoxicity against HEP-G2, HeLa, and HT-29 cancer cell lines, while showing no significant toxicity on normal HEK-293 cells. Conclusions: Overall, this research highlights tomato axillary shoots as a sustainable source of bioactive compounds, with potential applications in developing natural, plant-based prebiotic products that exhibit antifungal and antitumor activity. This research focuses on developing natural, plant-based prebiotic products with antifungal and cytotoxic effects. Full article
(This article belongs to the Section Nutrition and Public Health)
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21 pages, 727 KB  
Review
Can Myokines Serve as Supporters of Muscle–Brain Connectivity in Obesity and Type 2 Diabetes? Potential of Exercise and Nutrition Interventions
by Heaji Lee and Yunsook Lim
Nutrients 2025, 17(22), 3615; https://doi.org/10.3390/nu17223615 - 19 Nov 2025
Viewed by 1298
Abstract
Background/Objectives: Skeletal muscle–derived myokines have emerged as pivotal mediators of the muscle–brain axis, linking peripheral metabolic regulation with central nervous system function. These molecules may influence skeletal muscle maintenance, neuroplasticity, neuroinflammation, and cognitive performance, and their dysregulation is increasingly associated with metabolic and [...] Read more.
Background/Objectives: Skeletal muscle–derived myokines have emerged as pivotal mediators of the muscle–brain axis, linking peripheral metabolic regulation with central nervous system function. These molecules may influence skeletal muscle maintenance, neuroplasticity, neuroinflammation, and cognitive performance, and their dysregulation is increasingly associated with metabolic and cognitive impairment. In obesity (OB) and type 2 diabetes mellitus (T2DM), dysregulated myokine profiles characterized by reduced levels of irisin, brain-derived neurotrophic factor (BDNF), and cathepsin B (CTSB) have been reported and may contribute to the development of both sarcopenia and cognitive impairment. This review aims to summarize current evidence on myokine alterations in OB and T2DM and to evaluate how exercise- and nutrition-based interventions may modulate the muscle–brain axis to support metabolic and cognitive health. Methods: This narrative review synthesizes experimental, clinical, and translational studies examining (1) alterations in circulating myokines in OB and T2DM, (2) associations between myokines, skeletal muscle function, and neurocognitive outcomes, and (3) the modulatory effects of exercise and specific nutrients on myokine-mediated muscle–brain communication. Results: Available evidence indicates that OB and T2DM are frequently accompanied by reduced circulating levels of beneficial myokines such as irisin, BDNF, and CTSB, which may impair skeletal muscle integrity and contribute to cognitive decline. Restoring favorable myokine signaling through physical activity appears to enhance skeletal muscle maintenance, neuroplasticity, and metabolic homeostasis. Emerging data further suggest that selected nutrients can mimic or potentiate some exercise-induced myokine responses, thereby supporting both muscle and brain function. Collectively, these findings imply that combined exercise and nutrition strategies may exert synergistic or additive effects by reinforcing inter-organ communication along the muscle–brain axis. Conclusions: This review outlines current evidence on myokine alterations observed in OB and T2DM and discusses how exercise- and nutrition-based approaches may modulate the muscle–brain axis to mitigate metabolic dysfunction and preserve cognitive health. Targeting beneficial myokine pathways through tailored lifestyle interventions represents a promising avenue to support both skeletal muscle and neurocognitive function in individuals with metabolic disease. Full article
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24 pages, 2905 KB  
Article
Cafeteria and Fast-Food Diets Induce Neuroinflammation, Social Deficits, but a Different Cardiometabolic Phenotype
by Andrej Feješ, Petronela Sušienková, Lucia Mihalovičová, Veronika Kunšteková, Radana Gurecká, Veronika Borbélyová, Peter Celec and Katarína Šebeková
Nutrients 2025, 17(22), 3614; https://doi.org/10.3390/nu17223614 - 19 Nov 2025
Viewed by 552
Abstract
Background: Obesity is a risk factor for several non-communicable diseases and premature death. The Western-type diet, rich in calories and diverse in tastes, smells, and textures, promotes the onset and progression of obesity. We compared the effects of two Western-style palatable obesogenic diets—the [...] Read more.
Background: Obesity is a risk factor for several non-communicable diseases and premature death. The Western-type diet, rich in calories and diverse in tastes, smells, and textures, promotes the onset and progression of obesity. We compared the effects of two Western-style palatable obesogenic diets—the cafeteria (CAF) diet, which allows for self-selection of calorie-dense food items consumed by humans, and the fast-food diet (FFD)—composed of a fixed combination of cheeseburgers and fries—on the manifestation of obesity-related complications. Methods: 3-month-old female rats consumed either the control (CTRL), FFD, or CAF diet for 12 months. Body weight was monitored weekly. At the end of the experiment, rats underwent metabolic and behavioral testing. Cardiometabolic markers and those characterizing glycoxidative and carbonyl stress, inflammatory status, and tryptophan metabolism were determined. Results: The CAF rats gain most weight (CTRL: +111 ± 40 g; FFD: +211 ± 77 g; CAF: 316 ± 87 g). CAF feeding produced a classical metabolic syndrome–like profile with severe obesity, insulin resistance, dyslipidemia, and liver steatosis, whereas the FFD model led to moderate obesity with preserved insulin sensitivity but elevated blood pressure and hepatic cholesterol accumulation. Thus, the CAF group developed a severe metabolic syndrome-like pathology assessed as continuous metabolic syndrome z-core (CTRL: −2.3 ± 1.0; FFD: −0.4 ± 1.9; CAF: 3.0 ± 2.4). Despite these differences, both diets promoted neuroinflammation and social deficits, likely mediated through gut microbiota–derived metabolites such as 5-HIAA and indoxyl sulfate. Conclusions: In female rats, self-selected CAF diet drives more severe and distinct pattern of metabolic syndrome-like pathology than a fixed FFD. Full article
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38 pages, 7535 KB  
Article
Image-Based Dietary Energy and Macronutrients Estimation with ChatGPT-5: Cross-Source Evaluation Across Escalating Context Scenarios
by Marcela Rodríguez-Jiménez, Gustavo Daniel Martín-del-Campo-Becerra, Sandra Sumalla-Cano, Jorge Crespo-Álvarez and Iñaki Elio
Nutrients 2025, 17(22), 3613; https://doi.org/10.3390/nu17223613 - 19 Nov 2025
Viewed by 1110
Abstract
Background/Objectives: Estimating energy and macronutrients from food images is clinically relevant yet challenging, and rigorous evaluation requires transparent accuracy metrics with uncertainty and clear acknowledgement of reference data limitations across heterogeneous sources. This study assessed ChatGPT-5, a general-purpose vision-language model, across four [...] Read more.
Background/Objectives: Estimating energy and macronutrients from food images is clinically relevant yet challenging, and rigorous evaluation requires transparent accuracy metrics with uncertainty and clear acknowledgement of reference data limitations across heterogeneous sources. This study assessed ChatGPT-5, a general-purpose vision-language model, across four scenarios differing in the amount and type of contextual information provided, using a composite dataset to quantify accuracy for calories and macronutrients. Methods: A total of 195 dishes were evaluated, sourced from Allrecipes.com, the SNAPMe dataset, and Home-prepared, weighed meals. Each dish was evaluated under Case 1 (image only), Case 2 (image plus standardized non-visual descriptors), Case 3 (image plus ingredient lists with amounts), and Case 4 (replicates Case 3 but excluding the image). The primary endpoint was kcal Mean Absolute Error (MAE); secondary endpoints included Median Absolute Error (MedAE) and Root Mean Square Error (RMSE) for kcal and macronutrients (protein, carbohydrates, and lipids), all reported with 95% Confidence Intervals (CIs) via dish-level bootstrap resampling and accompanied by absolute differences (Δ) between scenarios. Inference settings were standardized to support reproducibility and variance estimation. Source stratified analyses and quartile summaries were conducted to examine heterogeneity by curation level and nutrient ranges, with additional robustness checks for error complexity relationships. Results and Discussion: Accuracy improved from Case 1 to Case 2 and further in Case 3 for energy and all macronutrients when summarized by MAE, MedAE, and RMSE with 95% CIs, with absolute reductions (Δ) indicating material gains as contextual information increased. In contrast to Case 3, estimation accuracy declined in Case 4, underscoring the contribution of visual cues. Gains were largest in the Home-prepared dietitian-weighed subset and smaller yet consistent for Allrecipes.com and SNAPMe, reflecting differences in reference curation and measurement fidelity across sources. Scenario-level trends were concordant across sources, and stratified and quartile analyses showed coherent patterns of decreasing absolute errors with the provision of structured non-visual information and detailed ingredient data. Conclusions: ChatGPT-5 can deliver practically useful calorie and macronutrient estimates from food images, particularly when augmented with standardized nonvisual descriptors and detailed ingredients, as evidenced by reductions in MAE, MedAE, and RMSE with 95% CIs across scenarios. The decline in accuracy observed when the image was omitted, despite providing detailed ingredient information, indicates that visual cues contribute meaningfully to estimation performance and that improvements are not solely attributable to arithmetic from ingredient lists. Finally, to promote generalizability, it is recommended that future studies include repeated evaluations across diverse datasets, ensure public availability of prompts and outputs, and incorporate systematic comparisons with non-artificial-intelligence baselines. Full article
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14 pages, 513 KB  
Article
Longitudinal Association Between Menopausal Transition and Obstructive Sleep Apnea with Effect Modification by Salt Intake: A Prospective Cohort Study
by Sujeong Shin, Yoonyoung Jang, Yoosoo Chang and Seungho Ryu
Nutrients 2025, 17(22), 3612; https://doi.org/10.3390/nu17223612 - 19 Nov 2025
Viewed by 570
Abstract
Background/Objectives: As high salt intake may increase obstructive sleep apnea (OSA) risk through fluid retention and upper airway narrowing during sleep, we aimed to determine whether dietary salt intake modified the association between menopausal transition and the risk of OSA. Methods: [...] Read more.
Background/Objectives: As high salt intake may increase obstructive sleep apnea (OSA) risk through fluid retention and upper airway narrowing during sleep, we aimed to determine whether dietary salt intake modified the association between menopausal transition and the risk of OSA. Methods: Between 2014 and 2018, we conducted a longitudinal study of 2572 women aged 42–52 years at the Kangbuk Samsung Hospital Total Healthcare Center. The participants were followed up until the end of 2024. OSA risk was evaluated using the STOP-Bang questionnaire, with a body mass index threshold adjusted to ≥30 kg/m2 in accordance with a Korean validation study. Dietary salt intake was categorized into tertiles, with tertile 3 representing the highest salt intake. Generalized estimating equations with time-dependent covariates were used to account for repeated measurements over time. Results: OSA risk increased during menopausal transition. Compared with the pre-menopausal stage, both late transition (β = 0.41, 95% confidence interval [CI] 0.05–0.78) and post-menopause (β = 0.61, 95% CI 0.20–1.02) were significantly associated with an increased risk of OSA, independent of potential confounders. A high salt intake (tertile 3) was also significantly associated with OSA. A significant interaction was observed between menopausal transition and salt intake (p = 0.040), with a stronger association between menopausal transition and OSA during the early transition stage in women with higher salt intake. Conclusions: Menopausal transition and high dietary salt intake appear to act synergistically to increase the risk of OSA in middle-aged women. Our results suggest that implementing targeted screening and reducing dietary salt intake may mitigate the risk of OSA during menopausal transition. Full article
(This article belongs to the Section Nutrition in Women)
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15 pages, 1112 KB  
Article
Stimulatory Effects of (+)-Epicatechin on Short- and Long-Term Memory in Aged Rats: Underlying Mechanisms
by Israel Ramirez-Sanchez, Veronica Salas-Gutierrez, Rosa Ordoñez-Razo, Pilar Ortiz-Vilchis, Claudia Calzada-Mendoza, Veronica Najera, Patricia Mendoza-Lorenzo, Guillermo Ceballos and Francisco Villarreal
Nutrients 2025, 17(22), 3611; https://doi.org/10.3390/nu17223611 - 19 Nov 2025
Viewed by 561
Abstract
Background/Objectives: We previously reported that the flavanol (+)-epicatechin (+Epi) enhances adult mice short-term working memory and neurogenesis. This study aimed to characterize the effects of +Epi on short- and long-term memory, to modulate mitochondria structure/function, oxidative stress (OS) and inflammation associated cytokines [...] Read more.
Background/Objectives: We previously reported that the flavanol (+)-epicatechin (+Epi) enhances adult mice short-term working memory and neurogenesis. This study aimed to characterize the effects of +Epi on short- and long-term memory, to modulate mitochondria structure/function, oxidative stress (OS) and inflammation associated cytokines in the hippocampus and pre-frontal cortex of aged rats. Methods: Experiments were conducted using aged (23 month old) male Sprague Dawley rats. The control group (n = 6/group) were exposed to vehicle (water) only while the treated group, was provided +Epi at 1 mg/kg/day by oral gavage for 8 weeks. Open-field recognition tests were used to evaluate short- and long-term memory. The hippocampus and frontal cortex were sampled and citrate synthase activity, ATP levels, mitochondrial proteins, cytokines (IL-1β, IL-6, TNF-a and IL-11), protein carbonylation, lipid peroxidation (malonaldehyde; MDA), superoxide dismutase 2 (SOD2), glutathione peroxidase (GPx) and catalase activity were quantified. Results: There was a significant improvement in both short- and long-term memory in the +Epi treated group vs. controls. Mitochondrial bioenergetics also improved with treatment as determined by increased citrate synthase activity and ATP content. Relative levels of the mitochondrial proteins mitofilin and complex V increased with +Epi. +Epi suppressed protein carbonyls and MDA levels. OS buffering systems were significantly enhanced with +Epi as per increases in SOD2, GPx and catalase enzyme activities. +Epi also decreased pro-inflammatory and stimulated anti-inflammatory cytokines vs. controls. Conclusions: Results demonstrate +Epi improves mitochondrial function, reduces OS and inflammation in the hippocampus and cortex leading to improved short- and long-term memory in aged animals providing evidence for possible mechanisms of action. Full article
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13 pages, 834 KB  
Article
Salivary Total Antioxidant Capacity of Sportive Adolescents—The Effect of Antioxidant Vitamin Intake with Usual Diet and Physical Exercises
by Anna Gawron-Skarbek, Adam Marek Wróblewski, Jacek Chrzczanowicz, Dariusz Nowak and Tomasz Kostka
Nutrients 2025, 17(22), 3610; https://doi.org/10.3390/nu17223610 - 19 Nov 2025
Viewed by 396
Abstract
Background: The body requires effective antioxidant defense mechanisms to counter the effect of oxidative stress. The aim of the study was to evaluate the postprandial effect of antioxidative vitamin (C, E and β-carotene) consumption during breakfast and of aerobic exercise on salivary total [...] Read more.
Background: The body requires effective antioxidant defense mechanisms to counter the effect of oxidative stress. The aim of the study was to evaluate the postprandial effect of antioxidative vitamin (C, E and β-carotene) consumption during breakfast and of aerobic exercise on salivary total antioxidant capacity (TAC). Methods: Fifty-one healthy male adolescents were examined (13–18 years; 15.4 ± 1.6). Dietary interviews including vitamin C, E, and β-carotene intake were performed twice, once on the examination day and again the day before. Salivary TAC was assessed using the DPPH method (2.2-diphenyl-1-picryl-hydrazyl) and expressed as % of free radical reduction. Saliva samples were assayed at three subsequent time-points: fasting (DPPH 1), after a meal—breakfast—(DPPH 2), and after aerobic exercise training (DPPH 3). Results: DPPH 2 was higher than DPPH 1 (16.8 ± 7.5 vs. 14.9 ± 7.2% of reduction; p = 0.03), and no differences were noted between DPPH 2 and DPPH 3 (16.8 ± 7.5 vs. 16.3 ± 6.5%; p > 0.05), nor between DPPH 1 and DPPH 3. Subjects with higher BMI demonstrated higher values of DPPH at all time-points of the study (p < 0.05). In turn, neither the DPPH values nor the changes in DPPH were related to weekly exercise-related energy expenditure (p > 0.05). No singular DPPH index was associated with the level of vitamin E or β-carotene intake with meals on the day before the study; however, DPPH 1 (rho = −0.38; p < 0.01) and DPPH 2 (rho = −0.45; p < 0.001) negatively correlated with vitamin C intake on the day before examination. Conclusions: In physically active adolescents, daily vitamin C consumption decreased salivary TAC, and the consumption of antioxidant nutrients/vitamins as part of a regular breakfast directly enhanced the antioxidant capacity of saliva; nevertheless, subsequent physical exercise had no detectable impact. Full article
(This article belongs to the Special Issue The Role of Healthy Eating and Physical Activity in Longevity)
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18 pages, 1365 KB  
Review
Perioperative Nutrition in Pediatric Patients with Congenital Heart Disease and Heart Failure
by Jaime Silva-Gburek, Kelsey May, Bailey Walvoord, Catalina Lozano and Jorge A. Coss-Bu
Nutrients 2025, 17(22), 3609; https://doi.org/10.3390/nu17223609 - 19 Nov 2025
Viewed by 1040
Abstract
Optimal nutritional therapy is important for infants and children with perioperative congenital heart disease and heart failure. Medical providers face physiological and metabolic challenges when administering enteral and parenteral nutrition to these patients. Complications related to enteral nutrition can increase morbidity and mortality, [...] Read more.
Optimal nutritional therapy is important for infants and children with perioperative congenital heart disease and heart failure. Medical providers face physiological and metabolic challenges when administering enteral and parenteral nutrition to these patients. Complications related to enteral nutrition can increase morbidity and mortality, although outcomes are improved in those cases in which caloric and nutrient administration can be achieved. Consensus regarding feeding strategies and timing of nutritive care is lacking. This narrative review aims to summarize, analyze and discuss the most recent literature on nutritional therapy in perioperative congenital heart disease, heart failure and pediatric patients requiring mechanical circulatory support in the intensive care unit. We also present our own institution’s enteral feeding protocols and rationale for their use supported by evidence-based medicine. Full article
(This article belongs to the Special Issue Nutritional Intervention in the Intensive Care Unit: New Advances)
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14 pages, 301 KB  
Review
Dietary Habits and Nutritional Status in Ecuatorian Children Aged 1–11 Years: A Systematic Review Highlighting the Dual Burden of Malnutrition
by Keila S. Micoanski, Cristina Izquierdo-García, Alex S. Huacho-Jácome, María Trelis and Mónica Gozalbo
Nutrients 2025, 17(22), 3608; https://doi.org/10.3390/nu17223608 - 19 Nov 2025
Viewed by 716
Abstract
Background: Background: Childhood dietary habits are critical determinants of physical growth, cognitive development, and long-term health. In Ecuador, malnutrition remains a major public health concern, with both undernutrition and overweight/obesity affecting children—especially in rural and indigenous populations. Methods: This systematic review followed PRISMA [...] Read more.
Background: Background: Childhood dietary habits are critical determinants of physical growth, cognitive development, and long-term health. In Ecuador, malnutrition remains a major public health concern, with both undernutrition and overweight/obesity affecting children—especially in rural and indigenous populations. Methods: This systematic review followed PRISMA 2020 guidelines (PROSPERO ID: CRD420251080987). Searches were conducted in SciELO, Dialnet, and ScienceDirect (accessed August 2025) using Boolean operators to identify quantitative studies in Spanish or English published between 2018 and 2025 that assessed dietary habits and nutritional status in Ecuadorian children aged 1–11 years. Seventeen studies, including approximately 12,000 children, were included. Results: Prevalence of chronic undernutrition ranged from 15% to 35%, while overweight and obesity reached 20–30%. Undernutrition was higher among rural and indigenous children, whereas overweight predominated in urban and higher-income settings. Common dietary patterns included high consumption of ultra-processed foods and sugar-sweetened beverages, and insufficient intake of fruits, vegetables, and quality proteins. Maternal education, socioeconomic level, and school food environments were key determinants. Conclusions: Both malnutrition and overweight persist among Ecuadorian children, reflecting a nutrition transition influenced by socioeconomic and environmental factors. Context-specific public health actions are urgently needed, including school-based nutrition education, regulation of food marketing, improved access to affordable healthy foods, and community-level engagement to promote sustainable dietary habits. The included studies were mostly cross-sectional and often used non-validated dietary assessment tools, which may influence the reported prevalence estimates. Full article
(This article belongs to the Section Nutrition Methodology & Assessment)
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27 pages, 12063 KB  
Article
Luteolin Alleviates Vascular Senescence Through Retinoic Acid–Peroxisome Proliferator-Activated Receptor Signaling and Lipid Metabolism Remodeling Combined with Multi-Omics Analysis
by Huasong Bai, Hongchen Jin, Tong Liu, Yulong Yin, Hengyan Wang, Siyu Ruan, Yunliang Li and Zhanzhong Wang
Nutrients 2025, 17(22), 3607; https://doi.org/10.3390/nu17223607 - 19 Nov 2025
Viewed by 574
Abstract
Background: Although luteolin (Lut) is well recognized for its anti-inflammatory and antioxidant effects, its potential role in preventing vascular senescence remains underexplored in primary vascular aging. This study aimed to investigate the anti-vascular-aging effects of Lut in both cellular and murine aging models [...] Read more.
Background: Although luteolin (Lut) is well recognized for its anti-inflammatory and antioxidant effects, its potential role in preventing vascular senescence remains underexplored in primary vascular aging. This study aimed to investigate the anti-vascular-aging effects of Lut in both cellular and murine aging models and to elucidate its conserved molecular mechanisms across species. Methods: Canine and feline vascular endothelial cells (cVECs and fVECs) were subjected to doxorubicin-induced senescence, while senescence-accelerated mice prone 8 (SAMP8) received an 8-week dietary supplementation with Lut. Senescence markers, inflammatory cytokines, antioxidant activities, vascular biomechanics, and histological changes were assessed. Transcriptomic and metabolomic analyses were combined to identify molecular pathways. Statistical significance was determined by one-way analysis of variance with Tukey’s or Games–Howell post hoc tests (p < 0.05). Results: Lut markedly reduced senescence-associated β-galactosidase activity, suppressed interleukin-6 and matrix metalloproteinase expression (p < 0.05), and enhanced superoxide dismutase activity and nicotinamide adenine dinucleotide levels (p < 0.05) in cVECs, fVECs, and SAMP8 sera. In aged mice, Lut alleviated arterial wall thickening and vascular inflammation, improved vascular biomechanics and systemic oxygenation (p < 0.05), and attenuated cardiac and hepatic inflammatory infiltration. Multi-omics analyses in cVECs revealed that Lut targets aldehyde dehydrogenase 1 to increase 9-cis retinoic acid, thereby activating the retinol X receptor–peroxisome proliferator-activated receptor (PPAR) network, which accelerates lipid clearance and oxidation. Consistent activation of this pathway was validated in murine vascular transcriptomes. Conclusions: These findings demonstrate that Lut delays vascular aging by activating the retinoic acid–PPAR axis and reprogramming lipid metabolism. This conserved mechanism was consistently observed in doxorubicin-induced cVEC senescence and the SAMP8 model, underscoring the robustness of Lut’s action across distinct contexts of vascular aging. Full article
(This article belongs to the Section Phytochemicals and Human Health)
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10 pages, 250 KB  
Article
Mapping Iodine Sources for Human Nutrition in Portugal Considering Geography, Seasonality, and Processing: Milk and Plant-Based Milk Alternatives
by Sarai Isabel Machado, Ana Machado, Adriano A. Bordalo, Susana Roque, Nuno Borges and Joana Almeida Palha
Nutrients 2025, 17(22), 3606; https://doi.org/10.3390/nu17223606 - 18 Nov 2025
Viewed by 549
Abstract
Background: Iodine is a micronutrient essential for the synthesis of thyroid hormones and crucial throughout life. Milk is potentially one of the major contributors to iodine intake in many countries, including Portugal, due to its consumption patterns. Objectives: This study characterizes iodine content [...] Read more.
Background: Iodine is a micronutrient essential for the synthesis of thyroid hormones and crucial throughout life. Milk is potentially one of the major contributors to iodine intake in many countries, including Portugal, due to its consumption patterns. Objectives: This study characterizes iodine content seasonality in milk and plant-based milk alternatives commercially available in Portugal. Results: Milk products representing summer and winter seasonal pastures and plant-based alternatives were obtained from major Portuguese supermarkets. Iodine was quantified by the Sandell–Kolthoff reaction in 146 winter and 142 summer milk products, as well as in 128 plant-based alternatives. Cow’s milk contained relevant iodine levels (Md = 19.9, IQR = 9.9 µg/100 mL), with no influence of thermal processing, fat or lactose content, season, or being organic and/or from pasture. However, regional differences were observed. The iodine concentration in plant-based drinks was residual, except for four fortified products. Conclusions: This study provides evidence that milk is still a relevant source of iodine in Portugal, whereas most plant-based alternatives provide negligible iodine, unless fortified. Given shifts towards plant-based diets, monitoring iodine sources and adjusting health policies to fulfil nutritional requirements are pivotal to ensure iodine adequacy. Full article
(This article belongs to the Section Micronutrients and Human Health)
12 pages, 434 KB  
Article
Maternal Pressure and Frequent Use of Bottle During Feeding Moderate Infant Food Cue Reactivity over Time
by Lenka H. Shriver, Yu Chen, Cheryl Buehler, Laurie Wideman and Esther M. Leerkes
Nutrients 2025, 17(22), 3605; https://doi.org/10.3390/nu17223605 - 18 Nov 2025
Viewed by 425
Abstract
Background: Food cue reactivity (FCR) has been associated with a higher obesity risk, but little is known about what factors influence FCR during infancy. This study examined the moderating effects of maternal feeding styles and bottle feeding on the associations between 2-month [...] Read more.
Background: Food cue reactivity (FCR) has been associated with a higher obesity risk, but little is known about what factors influence FCR during infancy. This study examined the moderating effects of maternal feeding styles and bottle feeding on the associations between 2-month and 6-month FCR. Methods: Data came from 299 mother–infant dyads who participated in a larger early-obesity-risk study and provided information via online questionnaires (i.e., socio-demographics) and/or during lab visits (i.e., anthropometrics) prenatally and at 2 and 6 months postpartum. Food-related practices (i.e., bottle use, milk type), feeding styles and FCR were measured by previously validated mother-reported measures: Infant Feeding Practices, Infant Feeding Styles, and Baby Eating Behavior Questionnaires. A regression model with moderation effects between 2-month FCR, feeding styles, and bottle feeding on 6-month FCR was tested using Mplus, controlling for selected variables (i.e., milk type, infant birthweight). Maternal pressure interacted with 2-month FCR and bottle feeding to predict 6-month FCR. Results: The moderating role of higher pressure was significant only for infants who received most of their feeds via a bottle (“high” bottle feeding). No other interactions were significant. Maternal feeding pressure in combination with frequent bottle feeding further strengthens the positive association between early and late FCR in infancy. Conclusions: Given the previously established links between FCR and weight outcomes among children, reducing the controlling maternal feeding practice of pressure during feeding in infancy, especially among mothers who frequently bottle feed, might be an important intervention target for optimizing weight outcomes in the first year of life. Full article
(This article belongs to the Special Issue Infant and Toddler Feeding and Development)
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12 pages, 488 KB  
Article
Effects of Probiotic Short-Term Regiment on Oral Health Parameters in Children: A Pilot Randomized Controlled Trial
by Edouard Starck, Vanessa Machado, João Botelho, Luís Proença, Helena Barroso, Carla Ascenso and Cecília Rozan
Nutrients 2025, 17(22), 3604; https://doi.org/10.3390/nu17223604 - 18 Nov 2025
Viewed by 1072
Abstract
Background/Objectives: Streptococcus mutans (S. mutans) is a primary cariogenic bacterium contributing to biofilm acidogenicity and enamel demineralization. Conventional caries prevention relies mainly on mechanical plaque control and dietary modification, but probiotics have emerged as potential adjuncts for oral microbiota modulation. [...] Read more.
Background/Objectives: Streptococcus mutans (S. mutans) is a primary cariogenic bacterium contributing to biofilm acidogenicity and enamel demineralization. Conventional caries prevention relies mainly on mechanical plaque control and dietary modification, but probiotics have emerged as potential adjuncts for oral microbiota modulation. This pilot randomized controlled trial aimed to evaluate the short-term effects of a multi-strain probiotic containing Lactobacillus and Bifidobacterium on clinical and microbiological parameters associated with dental caries in children. Methods: A double-blind, randomized, placebo-controlled clinical trial was conducted in 40 children aged 6–14 years from a community setting. Participants were randomly allocated (1:1) to receive either probiotic or placebo lozenges for 30 days. Clinical assessments included the Gingival Index (GI), Plaque Index (PI), International Caries Detection and Assessment System (ICDAS), salivary pH, buffering capacity, and salivary S. mutans concentration. The study was preregistered (10.17605/OSF.IO/GKVUW) and ethically approved. Results: The intervention was well tolerated, with no adverse events reported and high participant acceptability. Despite there being no statistically significant differences in any clinical or microbiological parameter (p > 0.05), we found trends toward higher salivary pH, improved buffering capacity, and reduced S. mutans counts in the probiotic group. Conclusions: Short-term probiotic supplementation was safe and well accepted among children but did not produce statistically significant improvements in oral health parameters over 30 days. These findings highlight the feasibility of probiotic use in pediatric populations and support the need for larger, longer-term trials to clarify their potential role as adjuncts in caries prevention. Full article
(This article belongs to the Special Issue Probiotics and Prebiotics for Oral Health Improvement)
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33 pages, 1420 KB  
Review
Nutritional Supplements for Muscle Hypertrophy: Mechanisms and Morphology—Focused Evidence
by Andreea Maria Mănescu, Simona Ștefania Hangu and Dan Cristian Mănescu
Nutrients 2025, 17(22), 3603; https://doi.org/10.3390/nu17223603 - 18 Nov 2025
Viewed by 3924
Abstract
Nutritional supplementation is widely used in resistance training, yet assessment of “hypertrophy” is often confounded by body-composition surrogates. This narrative review, anchored in mechanistic plausibility, integrates trials reporting morphology-direct outcomes (ultrasound/MRI). Across 46 eligible trials, protein/essential amino acids (EAA) showed consistent benefits when [...] Read more.
Nutritional supplementation is widely used in resistance training, yet assessment of “hypertrophy” is often confounded by body-composition surrogates. This narrative review, anchored in mechanistic plausibility, integrates trials reporting morphology-direct outcomes (ultrasound/MRI). Across 46 eligible trials, protein/essential amino acids (EAA) showed consistent benefits when daily intake was <1.6 g·kg−1·day−1 or when per-meal leucine provision was <2–3 g; effects plateaued once intakes exceeded ~2.0 g·kg−1·day−1. Creatine monohydrate (3–5 g·day−1, with or without loading) produced measurable increases in muscle thickness or cross-sectional area in interventions lasting ≥8–12 weeks, mediated by enhanced training volume and quality. β-hydroxy-β-methylbutyrate (HMB, 3 g·day−1) demonstrated conditional utility during high training stress or caloric deficit, but was largely neutral in well-fed, resistance-trained cohorts. Adjuncts such as omega-3 fatty acids (1–2 g·day−1), citrulline (6–8 g pre-exercise), and collagen (10–15 g·day−1 plus vitamin C) primarily facilitated training tolerance, recovery, or connective-tissue adaptation, rather than driving hypertrophy directly. A tiered model is proposed: protein/EAA as the foundation, creatine as amplifier, HMB as conditional agent, and adjuncts as facilitators. Methodological heterogeneity, short intervention length, and inconsistent imaging protocols remain limiting factors, underscoring the need for standardized ultrasound/MRI and adequately powered, preregistered trials. Full article
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16 pages, 425 KB  
Article
A Cross-Sectional Study of the Relationship Between Dietary Micronutrient Intake, Cognition and Academic Performance Among School-Aged Children in Taabo, Côte d’Ivoire
by Achil Tia, Amoin G. Konan, Jonas Hauser, Kouassi Y. Ndri, Olivier Ciclet, Lasme E. Esso and Charlemagne Nindjin
Nutrients 2025, 17(22), 3602; https://doi.org/10.3390/nu17223602 - 18 Nov 2025
Viewed by 564
Abstract
Background: Adequate nutrient intake is crucial for cognitive development and academic performance in schoolchildren. This study assessed the association between dietary intake and both cognition and academic performance in school-aged children from Taabo, Côte d’Ivoire. Methods: A total of 252 schoolchildren aged 6–12 [...] Read more.
Background: Adequate nutrient intake is crucial for cognitive development and academic performance in schoolchildren. This study assessed the association between dietary intake and both cognition and academic performance in school-aged children from Taabo, Côte d’Ivoire. Methods: A total of 252 schoolchildren aged 6–12 years were randomly selected from seven public primary schools. Dietary intake was assessed using repeated 24 h recalls. Cognitive skills were evaluated using Raven’s Colored Progressive Matrices (RCPM) and academic performance was determined based on end-of-year school results. Results: More than half of the participants had adequate intakes of vitamin A (55.8%), vitamin B6 (61.0%), vitamin B12 (61.0%) and omega-3 fatty acids (70.1%), while most had inadequate intakes of iron (96.8%), zinc (100%), thiamin (99.2%), riboflavin (99.6%) and folate (96.8%). Thiamin, riboflavin, folate, iron, zinc and a nutrient blend (comprising iron, zinc, thiamin, riboflavin and folate) showed significant associations with RCPM scores (p < 0.05). Additionally, higher intakes of thiamin (AOR = 6.3; 95% CI: 2.5–16.0, p < 0.001) and riboflavin (AOR = 2.2; 95% CI: 1.5–7.8, p = 0.003) were associated with increased odds of better cognitive performance compared with lower intakes. No consistent associations were found with academic performance. Conclusions: Compared to recommendations, intakes of several micronutrients were inadequate in most of the children. While thiamin, riboflavin, folate, iron, zinc and the nutrient blend showed significant associations with cognition, no association was found with academic performance. Further studies exploring such links are needed, especially those involving interventions. Full article
(This article belongs to the Special Issue Hot Topics in Clinical Nutrition (3rd Edition))
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37 pages, 2074 KB  
Review
Hidden Hunger in Pediatric Obesity: Redefining Malnutrition Through Macronutrient Quality and Micronutrient Deficiency
by Vanessa Nadia Dargenio, Nicoletta Sgarro, Giovanni La Grasta, Martina Begucci, Stefania Paola Castellaneta, Costantino Dargenio, Leonardo Paulucci, Ruggiero Francavilla and Fernanda Cristofori
Nutrients 2025, 17(22), 3601; https://doi.org/10.3390/nu17223601 - 18 Nov 2025
Cited by 1 | Viewed by 1095
Abstract
Background: Pediatric obesity exemplifies the paradox of energy excess coexisting with nutritional inadequacy. Despite high caloric intake, children with obesity often display deficiencies in essential macro- and micronutrients that impair growth, metabolic regulation, and long-term health. This review critically examines the mechanisms underlying [...] Read more.
Background: Pediatric obesity exemplifies the paradox of energy excess coexisting with nutritional inadequacy. Despite high caloric intake, children with obesity often display deficiencies in essential macro- and micronutrients that impair growth, metabolic regulation, and long-term health. This review critically examines the mechanisms underlying malnutrition in pediatric obesity, emphasizing the interplay between dietary quality, inflammation, microbiota alterations, and biomarker profiles, and identifies research gaps limiting precision nutrition approaches. Methods: A comprehensive narrative review of studies addressing macro- and micronutrient intake, metabolic and inflammatory biomarkers, and gut microbiota–host interactions in pediatric obesity was conducted. Evidence from both clinical and experimental models was integrated to evaluate mechanistic pathways, diagnostic criteria, and preventive strategies. Results: Obesity-related malnutrition arises from poor dietary quality, systemic inflammation, and microbiota dysbiosis, leading to impaired nutrient utilization and metabolic dysfunction. Deficiencies in vitamin D, calcium, iron, magnesium, and B vitamins are common and often coexist with macronutrient imbalances. Diets rich in saturated fats and refined carbohydrates exacerbate inflammation and metabolic risk, whereas plant-based proteins, unsaturated fats, and fiber support metabolic resilience. Precision nutrition and biomarker-guided monitoring show promise but require validation in pediatric cohorts. Evidence on microbiota modulation and nutrient–gene interactions remains inconsistent, reflecting methodological heterogeneity. Conclusions: Malnutrition in pediatric obesity should be recognized as a distinct clinical phenotype characterized by qualitative nutrient deficiency within a state of energy surplus. Addressing this paradox demands harmonized diagnostic criteria, longitudinal biomarker surveillance, and individualized dietary strategies informed by genetics and microbiome profiling. Multilevel interventions, linking clinical practice, policy, and food system reform, are essential to prevent lifelong metabolic complications and promote healthy growth trajectories. Full article
(This article belongs to the Section Pediatric Nutrition)
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20 pages, 1130 KB  
Review
Infantile Colic: When to Suspect Cow’s Milk Allergy
by Yvan Vandenplas, Silvia Salvatore, Mario C. Vieira, Francesco Savino, Ralf G. Heine, Koen Huysentruyt and Rosan Meyer
Nutrients 2025, 17(22), 3600; https://doi.org/10.3390/nu17223600 - 18 Nov 2025
Viewed by 1861
Abstract
Background/Objectives: Worldwide, an estimated 20–30% of infants suffer from infant colic (IC), with excessive crying and unsettled behavior, during the first three months of life. These infants are often referred for a medical evaluation, but the pathogenesis of IC remains poorly understood. The [...] Read more.
Background/Objectives: Worldwide, an estimated 20–30% of infants suffer from infant colic (IC), with excessive crying and unsettled behavior, during the first three months of life. These infants are often referred for a medical evaluation, but the pathogenesis of IC remains poorly understood. The aim of this narrative review is to critically appraise the available literature regarding the relation between IC and cow’s milk allergy (CMA). Methods: A literature search using the search strings cow’s milk allergy [MeSH Terms] OR food allergy [MesH Terms] AND colic [MeSH Terms] OR crying [MeSH Terms], limited to the English language, from inception to 15 June 2025, resulted in the identification of 135 articles. Of these, 18 clinical trials assessed the effect of a cow’s milk elimination diet on IC. Results: The role of CMA in IC in the absence of other allergic manifestations remains uncertain. However, when standard treatment of infant colic has failed and when other allergic symptoms are present, CMA may be considered. A diagnostic elimination diet which includes a 2–4-week trial of maternal cow’s milk elimination in breastfed infants or an extensively hydrolyzed cow’s milk or hydrolyzed rice formula should be performed. If the elimination diet results in a significant decrease in symptoms, reintroduction of cow’s milk protein into the diet is mandatory to fulfill the diagnostic criteria of CMA. Conclusions: Considering the limited current evidence, future research should prioritize large well-designed clinical trials with a focus on investigating CMA in colicky breastfed and formula-fed infants. Full article
(This article belongs to the Section Pediatric Nutrition)
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