Nutrients

13 pages, 323 KB

Open AccessArticle

The Impact of Physical Education Attendance and Diet on Bone Mineralization in Adolescents

by Agata Przytula and Joanna Popiolek-Kalisz

Nutrients 2025, 17(18), 3016; https://doi.org/10.3390/nu17183016 (registering DOI) - 20 Sep 2025

Background: Bone mineralization can be influenced by physical activity and dietary factors; however, the relative contributions of these factors are not well defined. Physical activity in adolescents can be implemented with physical education (PE) classes and there is no reported data on [...] Read more.

Background: Bone mineralization can be influenced by physical activity and dietary factors; however, the relative contributions of these factors are not well defined. Physical activity in adolescents can be implemented with physical education (PE) classes and there is no reported data on their impact on bone mineralization. This study investigates the relationship between PE class attendance, dietary factors, and bone mineralization in adolescents. Methods: 57 adolescents (median age 17.00 [16.00–17.00] years) were enrolled in this study. Bone mineralization was assessed with bioelectrical impedance analysis (BIA) and dietary knowledge and selected foods intakes were assessed with a validated questionnaire. Results: PE attendance and dietary knowledge were positively associated with bone mineralization in the multivariate models (R² = 0.85, p < 0.001 for the best model, and R² = 0.81, p < 0.001 for the simplified model), and PE attendance was the dominant positive factor among the modifiable ones (B = 0.20, p = 0.02, and B = 0.25, p = 0.004, respectively). The impact of calcium sources intake was diminished when controlled for PE class attendance. Conclusions: PE attendance is one of the key factors of bone mineralization in adolescents. Our study showed that the role of calcium sources intake was diminished when acknowledging physical activity data, however dietary knowledge remained a significant predictor. Enhancing dietary knowledge and promoting physical activity are key targets for improving bone health. Full article

(This article belongs to the Special Issue Association between Nutrition, Diet Quality, Dietary Patterns, and Human Health and Diseases—2nd Edition)

18 pages, 738 KB

Open AccessArticle

Predictors of Weight Reduction in a Multidisciplinary Community Program for Children with Overweight and Obesity: A Study from Emilia-Romagna, Italy

by Gianmarco Imperiali, Cecilia Acuti Martellucci, Marina Fridel, Giuseppe Diegoli, Maurizio Iaia, Giuliano Carrozzi, Petra Bechtold, Maria Elena Flacco and Lamberto Manzoli

Nutrients 2025, 17(18), 3015; https://doi.org/10.3390/nu17183015 (registering DOI) - 20 Sep 2025

Abstract

Background: The worldwide prevalence of obesity in children and adolescents quadrupled in the past decades, becoming a public health priority. Following the recommendation by the Italian Minister of Health, the Emilia-Romagna Region started a community-based program aimed at reducing pediatric overweight through [...] Read more.

Background: The worldwide prevalence of obesity in children and adolescents quadrupled in the past decades, becoming a public health priority. Following the recommendation by the Italian Minister of Health, the Emilia-Romagna Region started a community-based program aimed at reducing pediatric overweight through children and family behavioral counseling on nutrition and physical activity. Methods: Children with excess weight, aged 2–17 years, and without severe diseases were visited five times by a multidisciplinary team, who provided dietary advice, exercise plans, and psychosocial support, according to Italian guidelines. The outcomes were the median pre–post change in Δ₃₀BMI (distance between children’s BMI and age- and sex-specific obesity threshold values) and the proportion of children who moved to a lower weight class. Logistic regression was used to identify potential predictors of weight improvement. Results: Up to March 2025, 1331 participants completed the follow-up. In total, 17.5% of the children showed an improvement in weight class, and 32.5% had a reduction of more than one unit of Δ₃₀BMI. The program was significantly and substantially more effective among the children with obesity at baseline (overweight vs. obese children adjusted odds ratio—aOR—of weight class improvement: 0.28; p < 0.001), older than eight years (9–11 years vs. 2–8 years children aOR: 1.41; p < 0.05), who adhered to breakfast recommendations (aOR: 1.60; p < 0.01) and had no obese parents (≥1 vs. 0 obese parents aOR: 0.62; p < 0.05). Conclusions: The multidisciplinary model was associated with an overall positive impact on the weight status of the enrolled children. Given the varying response, however, in order to maximize cost-effectiveness, future programs could be reserved for children with obesity, older than eight years. Further randomized research is needed to investigate the efficacy of this intervention in different settings and on late clinical endpoints. Full article

(This article belongs to the Section Pediatric Nutrition)

► Show Figures

Figure 1

15 pages, 652 KB

Open AccessArticle

Exploring the Anti-Inflammatory Potential of a Mediterranean-Style Ketogenic Diet in Women with Lipedema

by Małgorzata Jeziorek, Angelika Chachaj, Andrzej Szuba, Dorota Różańska and Anna Prescha

Nutrients 2025, 17(18), 3014; https://doi.org/10.3390/nu17183014 (registering DOI) - 20 Sep 2025

Abstract

Background/Objectives: Lipedema is a chronic adipose tissue disorder characterized by disproportionate fat accumulation and inflammation, predominantly affecting women. While recent evidence suggests a systemic pro-inflammatory state in lipedema, the role of diet in modulating inflammation remains underexplored. This study assessed the anti-inflammatory potential [...] Read more.

Background/Objectives: Lipedema is a chronic adipose tissue disorder characterized by disproportionate fat accumulation and inflammation, predominantly affecting women. While recent evidence suggests a systemic pro-inflammatory state in lipedema, the role of diet in modulating inflammation remains underexplored. This study assessed the anti-inflammatory potential of a Mediterranean-style ketogenic diet and its effects after 7 months of adherence on systemic inflammation markers (CRP and IL-6) in women with lipedema (n = 24) and a control group with overweight/obesity (n = 24). Methods: The Dietary Inflammatory Index (DII) was used to characterize the inflammatory potential of the diet throughout the intervention. Dietary intake was analyzed pre- and post-intervention, and anthropometric, body composition, and biochemical parameters were measured. Results: Beyond its beneficial effects on body composition (significant reductions in body weight, fat, leg circumferences, and visceral fat), the intervention diet also demonstrated anti-inflammatory potential. In lipedema, baseline diet showed a pro-inflammatory DII profile (DII/day = 3.04), which was reduced by about 1.5 points after the intervention (p = 0.008). When expressed per 1000 kcal, the DII values were markedly lower for both baseline (DII = 0.22) and intervention diet (DII = ~0.01). Following the intervention diet, reduction in CRP (−0.39, p = 0.016) and IL-6 levels (−0.33, p = 0.034) in lipedema were observed. A significant positive association was observed between the intervention diet’s DII and CRP (r = 0.55, p = 0.005), and between the baseline diet’s DII and IL-6 (r = 0.50, p = 0.013) in lipedema group. Conclusions: These findings suggest that ketogenic diet rich in anti-inflammatory and antioxidant nutrients can reduce systemic inflammation in lipedema patients, independently of caloric restriction. Full article

(This article belongs to the Section Nutrition in Women)

► Show Figures

Figure 1

13 pages, 4733 KB

Open AccessArticle

Differential Associations of Vitamin D Metabolites with Adiposity and Muscle-Related Phenotypes in Korean Adults: Results from KNHANES 2022–2023

by Se-Hong Kim, Yuji Jeong, Seok-Won Son and Ha-Na Kim

Nutrients 2025, 17(18), 3013; https://doi.org/10.3390/nu17183013 (registering DOI) - 20 Sep 2025

Abstract

Background: Body composition plays a crucial role in metabolic health, aging, and the development of various diseases. Vitamin D has been implicated in the regulation of muscle and adipose tissue function, but its relationship with body composition remains unclear. Objectives: This study aimed [...] Read more.

Background: Body composition plays a crucial role in metabolic health, aging, and the development of various diseases. Vitamin D has been implicated in the regulation of muscle and adipose tissue function, but its relationship with body composition remains unclear. Objectives: This study aimed to investigate the association between serum 25-hydroxyvitamin D (25[OH]D) concentration, its subspecies, and body composition parameters and related phenotypes in Korean adults aged ≥20 years. Methods: This cross-sectional study included 2612 eligible participants from the Korea National Health and Nutrition Examination Survey 2022–2023, and data on body composition parameters and serum 25(OH)D levels were analyzed. Serum 25(OH)D levels and subspecies were determined using liquid chromatography-tandem mass spectrometry. Body composition factors included anthropometric indices, muscle strength, and fat and muscle mass, which were assessed using bioelectrical impedance analysis. Results: After multivariable adjustment, serum concentrations of total 25(OH)D and 25(OH)D₃ were inversely associated with waist circumference, body mass index, and fat mass and positively associated with handgrip strength and lean mass, whereas serum 25(OH)D₂ and 3-epi-25(OH)D₃ levels showed no such associations. Higher 25(OH)D₂ concentrations were positively associated with low muscle strength and the prevalence of sarcopenia. Through non-linear analyses, U-shaped correlations were identified between total 25(OH)D and 25(OH)D₃ levels with low muscle mass, respectively, while a J-shaped correlation was observed between 25(OH)D₂ level and low muscle mass. Conclusions: Total 25(OH)D and 25(OH)D₃ levels were inversely associated with adiposity and positively associated with muscle strength and lean mass, whereas the 25(OH)D₂ level was linked to low muscle strength and sarcopenia. The U- and J-shaped associations with low muscle mass indicate the complex and differential roles of vitamin D subspecies, warranting further study. Full article

(This article belongs to the Section Micronutrients and Human Health)

► Show Figures

Figure 1

22 pages, 423 KB

Open AccessReview

Ultra-Processed Food Consumption and Systemic Inflammatory Biomarkers: A Scoping Review

by Jacopo Ciaffi, Luana Mancarella, Claudio Ripamonti, Veronica Brusi, Federica Pignatti, Lucia Lisi and Francesco Ursini

Nutrients 2025, 17(18), 3012; https://doi.org/10.3390/nu17183012 (registering DOI) - 20 Sep 2025

Abstract

Background/Objectives: The consumption of ultra-processed foods (UPF) has increased worldwide and has been hypothesized to contribute to chronic diseases, including conditions characterized by inflammatory dysregulation. We conducted a scoping review to map the human evidence on the relationship between UPF consumption and systemic [...] Read more.

Background/Objectives: The consumption of ultra-processed foods (UPF) has increased worldwide and has been hypothesized to contribute to chronic diseases, including conditions characterized by inflammatory dysregulation. We conducted a scoping review to map the human evidence on the relationship between UPF consumption and systemic inflammatory biomarkers. Methods: We developed a search strategy combining terms for UPF with terms for circulating inflammatory biomarkers, including C-reactive protein (CRP/hs-CRP), interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), interleukin-8 (IL-8), monocyte chemoattractant protein-1 (MCP-1), plasminogen activator inhibitor-1 (PAI-1), and leptin. Findings were synthesized separately for children/adolescents and adults. Results: A total of 24 studies were included. CRP/hs-CRP was assessed in 21; IL-6 in 9; TNF-α in 8; IL-1β in 5; leptin in 5; MCP-1 in 5; PAI-1 in 5; and IL-8 in 2. In children/adolescents, CRP/hs-CRP tended to be higher with greater UPF intake in large cohorts and in preterm infants, whereas smaller or clinically selected samples did not show an association. For other biomarkers, IL-6 generally did not vary with UPF, TNF-α and IL-1β showed no association across studies, and the two IL-8 analyses yielded mixed results. In adults, 11/17 analyses reported higher CRP/hs-CRP levels with greater UPF intake, 5/17 reported no association, and 1/17 reported an association limited to women. IL-6 was predominantly higher with greater UPF intake; TNF-α likewise tended to be higher with UPF across several settings; IL-1β showed no association; MCP-1 and PAI-1 provided limited, inconsistent signals; leptin results were mixed. Conclusions: Higher UPF consumption is frequently associated with elevated systemic inflammatory biomarkers—most consistently CRP/hs-CRP—across adults and selected pediatric contexts. Signals for IL-6 and TNF-α appear in specific populations, whereas IL-1β, MCP-1, PAI-1, and leptin show inconsistent patterns. Full article

(This article belongs to the Special Issue Mechanisms of Ultra-Processed Foods and Health Outcomes)

16 pages, 340 KB

Open AccessArticle

Time-Restricted Eating Without Exercise Enhances Anaerobic Power and Reduces Body Weight: A Randomized Crossover Trial in Untrained Adults

by Zifu Yu and Takeshi Ueda

Nutrients 2025, 17(18), 3011; https://doi.org/10.3390/nu17183011 (registering DOI) - 20 Sep 2025

Abstract

Background: Time-restricted eating (TRE), a dietary strategy that confines daily food intake to specific time windows, has been shown in animal models to enhance physical performance even without exercise training. However, evidence in humans under non-exercise conditions remains limited. Objective: This [...] Read more.

Background: Time-restricted eating (TRE), a dietary strategy that confines daily food intake to specific time windows, has been shown in animal models to enhance physical performance even without exercise training. However, evidence in humans under non-exercise conditions remains limited. Objective: This study aimed to investigate the effects of early TRE (eTRE; 08:00–14:00) and delayed TRE (dTRE; 12:00–18:00) on body weight, aerobic endurance, and anaerobic power in untrained adults. Methods: In a randomized crossover trial, 28 healthy university students (mean age 23.47 ± 2.87 years; 16 women) completed two 4-week interventions, eTRE and dTRE, separated by a 2-week washout. Participants did not engage in any structured exercise during the intervention period. Body weight, aerobic endurance (cycling time to exhaustion at a fixed workload), and anaerobic power (peak power output during sprint cycling) were assessed after each phase. Results: Body weight significantly decreased after eTRE (−1.56 kg; 95% CI [−2.07, −1.05]; p < 0.001; Cohen’s

d_{z}

= 1.17) and dTRE (−0.61 kg; 95% CI [−1.12, −0.10]; p = 0.022; Cohen’s

d_{z}

= 0.55), with a greater reduction observed in eTRE compared to dTRE (−0.95 kg; 95% CI [−1.74, −0.16]; p = 0.020). Aerobic endurance showed no significant change across phases (all p > 0.05). Anaerobic power significantly improved after both eTRE (+21.25 W; 95% CI [12.03, 30.47]; p < 0.001; Cohen’s

d_{z}

= 1.10) and dTRE (+35.43 W; 95% CI [26.21, 44.65]; p < 0.001; Cohen’s

d_{z}

= 1.20), and the improvement was significantly greater in dTRE compared to eTRE (+14.18 W; 95% CI [1.79, 26.57]; p = 0.025; Cohen’s

d_{z}

= 0.54). Conclusions: Both early and delayed TRE independently led to weight loss and enhanced anaerobic power in the absence of an exercise intervention. eTRE was more effective for weight reduction, whereas dTRE produced greater improvements in anaerobic performance. Full article

(This article belongs to the Section Sports Nutrition)

► Show Figures

Figure 1

11 pages, 216 KB

Open AccessArticle

Dietary Inflammatory Index and Nutritional Status in Children with Inborn Errors of Metabolism on Protein-Restricted Diets

by Emine Aktaş, Betül Çiçek, Ilyas Okur, Asli İnci and Leyla Tümer

Nutrients 2025, 17(18), 3010; https://doi.org/10.3390/nu17183010 (registering DOI) - 20 Sep 2025

Abstract

Background: The primary treatment for inborn errors of metabolism (IEM) involves restricted intake of natural protein. Inadequate diets can lead to an increased risk of inflammation and susceptibility to infections. The Dietary Inflammatory Index (DII) is used to estimate whether a diet [...] Read more.

Background: The primary treatment for inborn errors of metabolism (IEM) involves restricted intake of natural protein. Inadequate diets can lead to an increased risk of inflammation and susceptibility to infections. The Dietary Inflammatory Index (DII) is used to estimate whether a diet has anti-inflammatory or pro-inflammatory properties. This study aimed to investigate the relationship between the inflammatory index score of natural protein-restricted diets used in medical nutrition therapy for IEM intoxication, the anthropometric measurements and nutritional status of affected children. Method: The study included 20 patients (5 organic acidemia, 5 urea cycle disorders, 10 phenylketonuria) and 20 healthy children. Patients followed a natural protein-restricted diet, while the healthy control group maintained their usual dietary habits. Dietary records were collected for both groups, and the DII and macro-micronutrient intakes were calculated. Result: DII scores were similar between the patient and control groups. Anthropometric measurements did not differ significantly between the groups. However, carbohydrate and fat intakes were higher in the patient group compared to the control group (p < 0.05). Additionally, comparative analyses revealed that vitamin B1, C and E, iron, and magnesium intakes were higher in the patient group than in the control group. Conclusions: Children on a natural protein-restricted diet showed growth patterns comparable to their healthy peers. This study demonstrated that nutritional deficiencies can be prevented in amino acid metabolism disorders treated with a natural protein-restricted diet by carefully controlling nutrition with vitamin and mineral-fortified formulas. Full article

(This article belongs to the Section Pediatric Nutrition)

29 pages, 915 KB

Open AccessReview

Gut Microbiota and Food Allergy: A Review of Mechanisms and Microbiota-Targeted Interventions

by Roxana Cristina Mareș, Maria Oana Săsăran and Cristina Oana Mărginean

Nutrients 2025, 17(18), 3009; https://doi.org/10.3390/nu17183009 (registering DOI) - 20 Sep 2025

Abstract

Background: Food allergies (FAs) have become a major public health concern worldwide, with rising prevalence particularly among children. Traditional genetic and allergen exposure models do not fully explain this increase, prompting growing interest in the role of the gut microbiota. Early-life microbial [...] Read more.

Background: Food allergies (FAs) have become a major public health concern worldwide, with rising prevalence particularly among children. Traditional genetic and allergen exposure models do not fully explain this increase, prompting growing interest in the role of the gut microbiota. Early-life microbial colonization is now recognized as a critical determinant of immune development, with disruptions in microbial balance implicated in allergic sensitization. Objective: This review aims to synthesize recent human studies investigating the relationship between gut microbiota composition and food allergies, with an emphasis on underlying mechanisms and the potential of microbiota-targeted interventions. Methods: A literature search was conducted, including eligible studies concerning gut microbiota and food allergy. A total of 31 studies met the inclusion criteria. Results: The findings indicate that early-life factors, including delivery mode, feeding practices, antibiotic exposure, and environmental microbial diversity, have a significant influence on gut microbial colonization. Allergic children consistently exhibit reduced microbial diversity and lower levels of beneficial taxa such as Bifidobacteria, Faecalibacteria, and Clostridia. Microbial dysbiosis is associated with enhanced type 2 immune responses, reduced regulatory T cell activity, and altered profiles of short-chain fatty acids. Dietary modulation through prebiotics, probiotics, and synbiotics has shown potential in restoring microbial balance and promoting immune tolerance, although with varying degrees of efficacy depending on the strains, formulas, and timing of intervention. Conclusions: The gut microbiota plays a central role in the pathogenesis and potential prevention of food allergies. Microbiota-targeted dietary strategies, particularly in early life, offer promising avenues for promoting immune tolerance. Full article

(This article belongs to the Special Issue Nutrition and Gut Microbiome in the Prevention of Food Allergy)

► Show Figures

Figure 1

17 pages, 869 KB

Open AccessArticle

A 12-Week Randomized Controlled Trial of Nutrition and Exercise Education with Dietary Supplementation for Sarcopenia Prevention in Korean Baby Boomers

by Eun-Hee Jang and Seungmin Lee

Nutrients 2025, 17(18), 3008; https://doi.org/10.3390/nu17183008 (registering DOI) - 20 Sep 2025

Abstract

Background/Objectives: Sarcopenia is a major public health concern, and early preventive strategies in midlife are increasingly emphasized. The goal of this study was to evaluate the effectiveness of a 12-week lifestyle intervention that included nutrition education, exercise education, and dietary supplementation in [...] Read more.

Background/Objectives: Sarcopenia is a major public health concern, and early preventive strategies in midlife are increasingly emphasized. The goal of this study was to evaluate the effectiveness of a 12-week lifestyle intervention that included nutrition education, exercise education, and dietary supplementation in Korean baby boomers. Methods: This single-blind, randomized controlled trial included 42 Korean baby boomers born between 1955 and 1963. Participants were randomly assigned to one of three groups: (1) nutrition and exercise education plus dietary supplementation (DiExSp), (2) nutrition and exercise education only (DiEx), or (3) control. The intervention was delivered online, and the DiExSp group additionally received a daily supplement containing protein, vitamins, and minerals. Primary outcomes included dietary intake, body composition, physical function, and fasting blood biomarkers. Nonparametric tests and effect size calculations were performed using SAS version 9.4. Results: A total of 31 participants completed the study. The results showed significant increases in protein and micronutrient intake (p < 0.05), with large effect sizes in the DiExSp group. Handgrip strength showed improvement in both the DiExSp and DiEx groups (DiExSp: p = 0.027, r = 0.63; DiEx: p = 0.020, r = 0.73), while no changes were observed in other physical parameters. HbA1c levels showed a significant decrease in the DiExSp group compared to the control (p < 0.05, ε² = 0.01). No significant differences were observed for other biomarkers. Conclusions: A 12-week intervention combining dietary supplementation with education resulted in improved dietary intake, muscle strength, and glycemic control. However, most effects were confined to within-group changes. Between-group differences were minimal, and effect sizes were small. These findings provide preliminary evidence that lifestyle intervention strategies may contribute to sarcopenia prevention in midlife adults. Full article

(This article belongs to the Special Issue Nutrition and Exercise in the Prevention and Treatment of Chronic Diseases)

► Show Figures

Figure 1

16 pages, 11378 KB

Open AccessArticle

Manganese Deficiency Causes Testicular Developmental Disorders, Blood–Testis Barrier Damage, and Spermatogenesis Disruption via Nrf2-Mediated Oxidative Stress

by Dianyi Peng, Fuqing Feng, Heng Yin, Jianfei Zhao, Shanchuan Cao and Jingbo Liu

Nutrients 2025, 17(18), 3007; https://doi.org/10.3390/nu17183007 (registering DOI) - 20 Sep 2025

Abstract

Background: Manganese (Mn) is a trace element essential for multiple physiological and biological processes. The testis plays a key role in male reproduction by producing sperm and synthesizing male hormones. This study investigates how Mn deficiency affects testicular development, spermatogenesis, and the [...] Read more.

Background: Manganese (Mn) is a trace element essential for multiple physiological and biological processes. The testis plays a key role in male reproduction by producing sperm and synthesizing male hormones. This study investigates how Mn deficiency affects testicular development, spermatogenesis, and the blood–testis barrier (BTB), and evaluates associated variations in oxidative stress to explore potential mechanisms. Methods: A Mn-deficient diet was used to induce Mn deficiency in mice, with MnCl₂ administered via intraperitoneal injection. Mn levels in testicular tissue were measured by atomic absorption spectrometry. Testis and sperm morphology were assessed by H.E. and sperm staining. BTB markers were analyzed using immunofluorescence, Western blot, and qPCR. Oxidative stress was evaluated biochemically. Nrf2 pathway changes were detected by qPCR and Western blot. Results: The results indicated that Mn deficiency dramatically decreased the testicular index, caused abnormal testicular tissue structure, and significantly decreased Johnsen’s score. At the same time, sperm density and motility were significantly reduced, and the sperm deformity rate was significantly increased. In addition, the BTB function was impaired, as indicated by the significantly down-regulated expression of tight junction proteins including Occludin, ZO-1, JAM-A, and Claudin-11. As the oxidative stress levels increased, the mRNA and protein expression levels of molecules (including Nrf2 and HO-1) related to the Nrf2 signaling pathway were significantly down-regulated, while its inhibitor Keap1 exhibited significantly up-regulated expression. Notably, after supplementing MnCl₂, all the above abnormal indicators were significantly improved. Conclusions: Mn deficiency can lead to testicular tissue damage, decreased sperm quality, and BTB dysfunction, and the potential mechanism is probably closely associated with the increase in the oxidative stress level mediated by the Nrf2 pathway. Full article

(This article belongs to the Special Issue A New Perspective: The Effect of Trace Elements on Human Health)

► Show Figures

Figure 1

25 pages, 8209 KB

Open AccessArticle

Identification of Amino Acids That Regulate Angiogenesis and Alter Pathogenesis of a Mouse Model of Choroidal Neovascularization

by Chenchen Li, Jiawen Wu, Yingke Zhao, Jing Zhu, Xinyu Zhu, Yan Chen and Jihong Wu

Nutrients 2025, 17(18), 3006; https://doi.org/10.3390/nu17183006 - 19 Sep 2025

Abstract

Background: Metabolic stress from amino acid (AA) insufficiency is increasingly linked to pathological angiogenesis, but specific essential AA (EAA) roles remain undefined. Neovascular age-related macular degeneration (AMD), a major cause of blindness driven by aberrant ocular neovascularization, has limited efficacy with current VEGFA-targeting [...] Read more.

Background: Metabolic stress from amino acid (AA) insufficiency is increasingly linked to pathological angiogenesis, but specific essential AA (EAA) roles remain undefined. Neovascular age-related macular degeneration (AMD), a major cause of blindness driven by aberrant ocular neovascularization, has limited efficacy with current VEGFA-targeting therapies. We sought to identify specific EAAs that regulate pathological angiogenesis and dissect their mechanisms to propose new therapeutic strategies. Methods: Human retinal microvascular endothelial cells (HRMVECs) were used to identify angiogenesis-regulating amino acids through systematic EAA screening. The molecular mechanism was investigated using shRNA-mediated knockdown of key stress response regulators (HRI, PKR, PERK, GCN2) and ATF4. Angiogenesis was assessed via tubule formation and migration assays. Therapeutic potential was examined in a laser-induced choroidal neovascularization (CNV) mouse model, evaluated by fluorescein angiography and histomorphometry. Results: Deprivation of methionine, lysine, and threonine potently induced capillary-like tube formation (p < 0.01). Mechanistically, restriction of these three EAAs activated HRI and GCN2 kinases, converging on eIF2α phosphorylation to induce ATF4 and its target VEGFA. Dual, but not single, knockdown of HRI and GCN2 abolished eIF2α-ATF4 signaling and angiogenic responses. Restricting these EAAs exacerbated CNV area in mice. Conclusion: Our findings reveal a coordinated HRI/GCN2-ATF4-VEGFA axis linking EAA scarcity to vascular remodeling, establishing proof-of-concept for targeting this pathway in CNV. This work highlights the therapeutic potential of modulating specific AA availability or targeting the HRI/GCN2-ATF4 axis to treat CNV. Full article

(This article belongs to the Section Proteins and Amino Acids)

14 pages, 885 KB

Open AccessArticle

Association Between Multi-Dimensional Sleep Health and Breakfast Skipping in Japanese High School Students

by Suzune Nagao, Yuh Sasawaki, Hitoshi Inokawa, Nobuko Kitagawa, Naoyuki Takashima and Kazuhiro Yagita

Nutrients 2025, 17(18), 3005; https://doi.org/10.3390/nu17183005 - 19 Sep 2025

Abstract

Background/Objectives: Breakfast skipping has been associated with a wide range of adverse health outcomes, including metabolic disorders, disrupted circadian rhythm, and impairments of memory and attention in adolescents and adults. Although partial associations between sleep and breakfast behaviors have been reported, few [...] Read more.

Background/Objectives: Breakfast skipping has been associated with a wide range of adverse health outcomes, including metabolic disorders, disrupted circadian rhythm, and impairments of memory and attention in adolescents and adults. Although partial associations between sleep and breakfast behaviors have been reported, few studies have examined multi-dimensional sleep health simultaneously in relation to breakfast skipping, especially comprehensive studies systematically examining this relationship, particularly under controlled social conditions, remain insufficient. Methods: We here demonstrate the association between sleep health and breakfast skipping among 2969 Japanese high school students. Participants provided between one and eight days of sleep diary data, including meal timing records; most (78.1%) completed all eight days, while the remainder contributed fewer days. Additionally, the Pittsburgh Sleep Quality Index (PSQI) was used to assess sleep quality, and the Pediatric Daytime Sleepiness Scale (PDSS) was used to evaluate daytime sleepiness. Results: Later wake-up times, lower sleep quality, and stronger daytime sleepiness were each associated with a higher likelihood of breakfast skipping. In additional analyses, no significant pairwise interactions were detected among wake-up time, PSQI, and PDSS, suggesting that these factors may be separately associated with breakfast skipping. Conclusions: These findings suggest that multi-dimensional sleep health, including wake-up time, sleep quality, and daytime sleepiness, is relevant to breakfast skipping. This study offers a novel contribution by linking multiple downstream indicators influenced by sleep health to breakfast behavior. Full article

(This article belongs to the Special Issue Body Image and Nutritional Status from Childhood to Adulthood)

6 pages, 162 KB

Open AccessEditorial

Precision Nutrition for Public Health

by Sabina Lachowicz-Wiśniewska and Agata Kotowska

Nutrients 2025, 17(18), 3004; https://doi.org/10.3390/nu17183004 - 19 Sep 2025

Abstract

Public health—understood as both a science and a practice aimed at preventing disease, prolonging life, and promoting health—has become one of the most critical domains of institutional action, shaped by both nation-states and international organizations [...] Full article

(This article belongs to the Special Issue Public Health, Nutritional Behavior and Nutritional Status)

17 pages, 1673 KB

Open AccessArticle

Fecal Short-Chain Fatty Acids to Predict Prediabetes and Type 2 Diabetes Risk: An Exploratory Cross-Sectional Study

by Rocío Puig, Marina Idalia Rojo-López, Josep Julve, Esmeralda Castelblanco, Julia Ponomarenko, Susana Amézqueta, Joan Vendrell, Josep Franch-Nadal, Josep Lluís Torres, Dídac Mauricio and Sara Ramos-Romero

Nutrients 2025, 17(18), 3003; https://doi.org/10.3390/nu17183003 - 19 Sep 2025

Abstract

Background/Objectives: Gut microbiota is profoundly influenced by dysglycemic states, including prediabetes (preDM) and type 2 diabetes (T2D). Although short-chain fatty acids (SCFAs) may serve as proxies reflecting these microbial changes, their predictive role remains elusive. This study aimed to evaluate the association between [...] Read more.

Background/Objectives: Gut microbiota is profoundly influenced by dysglycemic states, including prediabetes (preDM) and type 2 diabetes (T2D). Although short-chain fatty acids (SCFAs) may serve as proxies reflecting these microbial changes, their predictive role remains elusive. This study aimed to evaluate the association between fecal SCFA concentrations and glycemic status (preDM and T2D), using individuals with normoglycemia (NonDM) as the reference group in a Mediterranean adult population. Methods: This study included a total of 88 participants from the Di@bet.es study who were classified into three groups according to the American Diabetes Association criteria: NonDM (45%), preDM (27%), and T2D (28%), respectively. We evaluated gut microbiota populations through massive sequencing and determined SCFAs concentration using gas chromatography–mass spectrometry. Adjusted multiple logistic regression models were used to estimate associations between SCFAs and metabolic status. Results: The mean age of subjects with preDM and T2D was approximately 68 years, older than that of NonDM participants (about 60 years). About 50% of the subjects in the NonDM and preDM groups were female, whereas in the T2D group, females represented about 25%. The analysis revealed that only fecal acetic acid was significantly reduced in T2D compared to NonDM (p = 0.036) and preDM (p = 0.018) groups. Remarkably, fecal acetic acid was negatively associated with T2D risk when taking preDM as the reference state (OR = 0.561 [95% CI: 0.371–0.846], p = 0.009). Intriguingly, fecal acetic acid was identified as a significant positive predictor of preDM risk, taking the NonDM group as reference (OR = 1.422; p = 0.028), while propionic acid was inversely associated with preDM (OR = 0.714; p = 0.028). Conclusions: Our analysis showed that fecal acetic acid levels were associated with a reduced risk of T2D but also with an increased risk of preDM; however, the biological relevance of these findings remains uncertain. Full article

(This article belongs to the Section Nutrition and Diabetes)

► Show Figures

Figure 1

19 pages, 1055 KB

Open AccessReview

Omega-3 Polyunsaturated Fatty Acids and Cognitive Decline in Adults with Non-Dementia or Mild Cognitive Impairment: An Overview of Systematic Reviews

by Maria Inês Barros, Teresa Brandão, Susana Couto Irving, Paula Alves, Filomena Gomes and Marta Correia

Nutrients 2025, 17(18), 3002; https://doi.org/10.3390/nu17183002 - 19 Sep 2025

Abstract

Background/Objectives: As global aging accelerates, prevalence of mild cognitive impairment (MCI) continues to rise, challenging healthcare systems and diminishing older adults’ quality of life. There is great interest in better understanding the neuroprotective/anti-inflammatory properties of omega-3 polyunsaturated fatty acids but the results from [...] Read more.

Background/Objectives: As global aging accelerates, prevalence of mild cognitive impairment (MCI) continues to rise, challenging healthcare systems and diminishing older adults’ quality of life. There is great interest in better understanding the neuroprotective/anti-inflammatory properties of omega-3 polyunsaturated fatty acids but the results from many published studies in humans come to different conclusions. This review aims to clarify the efficacy of n-3 fatty acids as a preventive or therapeutic strategy for cognitive health and to inform future clinical recommendations within aging populations. Methods: Following PRISMA guidelines and a registered PROSPERO protocol, we reviewed systematic reviews (SRs) from 2014 to 2024 assessing exclusive n-3 fatty acid supplementation and cognitive outcomes via MMSE. Data were extracted on intervention details and cognitive scores. Meta-analyses used fixed and random-effects models, with Hedges’ estimating overall impact. Quality was assessed using AMSTAR-2, and statistical analyses were performed (SPSS 28). Results: A total of nine SRs incorporating 14 RCTs were included, representing 26,881 participants aged 40 years or older. The pooled random-effects meta-analysis showed a statistically significant but modest improvement in MMSE scores (effect size: 0.16; 95% CI: 0.01–0.32). Heterogeneity was moderate (I² = 42.8%), and no publication bias was detected. Further analyses revealed no significant associations between treatment duration or dosage and cognitive outcomes, suggesting a threshold effect rather than a dose–response relationship. Conclusions: These findings support n3-PUFA supplementation as a complementary approach to lifestyle-based strategies for cognitive health, including diet, physical activity, sleep optimization, and cognitive training. While benefits appear modest, consistent effects across studies warrant further high-quality research and well-designed studies to strengthen clinical recommendations. Full article

(This article belongs to the Special Issue Food as Medicine for Brain and Other Tissues)

► Show Figures

Figure 1

23 pages, 8073 KB

Open AccessArticle

Alfalfa Polysaccharide Alleviates Colitis by Regulating Intestinal Microbiota and the Intestinal Barrier Against the TLR4/MyD88/NF-κB Pathway

by Shaokai La, Muhammad Abaidullah, Hao Li, Yalei Cui, Boshuai Liu and Yinghua Shi

Nutrients 2025, 17(18), 3001; https://doi.org/10.3390/nu17183001 - 19 Sep 2025

Abstract

Background/Objectives: Ulcerative colitis (UC) pathogenesis involves gut barrier dysfunction, dysregulated immune responses, and gut microbiota imbalance. Alfalfa polysaccharide (APS), a bioactive compound with immunomodulatory potential, remains underexplored in intestinal inflammation. While APS exhibits anti-inflammatory properties in vitro, its in vivo efficacy, mechanisms, [...] Read more.

Background/Objectives: Ulcerative colitis (UC) pathogenesis involves gut barrier dysfunction, dysregulated immune responses, and gut microbiota imbalance. Alfalfa polysaccharide (APS), a bioactive compound with immunomodulatory potential, remains underexplored in intestinal inflammation. While APS exhibits anti-inflammatory properties in vitro, its in vivo efficacy, mechanisms, and ability to restore gut microbiota and barrier integrity in UC are unclear. This study aims to investigate the treatment effect of APS on dextran sulfate sodium (DSS)-induced colitis in mice and confirm its prebiotic potential. Methods: A mouse model of ulcerative colitis was induced by DSS. RNA sequencing, Western blotting, the terminal deoxynucleotidyl transferase dUTP nick end labeling technique, and an immuno-histochemical technique were used to study the mechanism of action by which APS at different dosages relieves DSS-induced colitis. Results: The findings show that APS alleviated the symptoms of colitis in mice given DSS, improved the gut morphology, heightened goblet cells production, increased the levels of IL-10 and IL-22, decreased the levels of TNF-α, IL-1β, and IL-6, and prevented the activation of the TLR4/MyD88/NF-κB pathways. Additionally, they maintained the integrity of the intestine by enhancing the expression of the mucins MUC2 and MUC5AC and by increasing the amounts of ZO-1, Occludin, and Claudin-1 proteins. Moreover, APS supported the growth of probiotic bacteria, including unclassified_f_lachnospiraceae, Parabacteroides, Alistipes, and Mucispirillum, and in particular, Parabacteroides distasonis, which is strongly associated with decreased pro-inflammatory cytokine through the inhibition of the TLR4-MyD88-NFκB pathways. Conclusions: APS can be used as a new type of prebiotic to improve UC by regulating intestinal flora and enhancing intestinal barrier function against the TLR4-MyD88-NFκB pathway. Full article

(This article belongs to the Section Nutrition and Metabolism)

► Show Figures

Figure 1

19 pages, 1945 KB

Open AccessSystematic Review

Effect of Apple Cider Vinegar Intake on Body Composition in Humans with Type 2 Diabetes and/or Overweight: A Systematic Review and Meta-Analysis of Randomized Controlled Trials

by Alberto Castagna, Yvelise Ferro, Francesca Rita Noto, Rossella Bruno, Analucia Aragao Guimaraes, Carmelo Pujia, Elisa Mazza, Samantha Maurotti, Tiziana Montalcini and Arturo Pujia

Nutrients 2025, 17(18), 3000; https://doi.org/10.3390/nu17183000 - 19 Sep 2025

Abstract

Background: Apple cider vinegar (ACV) is a naturally fermented beverage with potential metabolic health benefits; however, its effects on weight loss remain controversial. This systematic review and meta-analysis of randomized controlled trials (RCTs) was conducted to assess the effect of ACV on anthropometric [...] Read more.

Background: Apple cider vinegar (ACV) is a naturally fermented beverage with potential metabolic health benefits; however, its effects on weight loss remain controversial. This systematic review and meta-analysis of randomized controlled trials (RCTs) was conducted to assess the effect of ACV on anthropometric measurements in adults. Methods: We performed a systematic search of PubMed, Web of Science, Scopus, and CENTRAL up to March 2025 for randomized controlled trials (RCTs) in adults (≥18 years) evaluating the effects of ACV for ≥4 weeks on body composition parameters. Primary outcomes included changes in body weight, BMI, waist circumference, and other anthropometric measures. Risk of bias was assessed using the Revised Cochrane Risk-of-bias tool. Results: Out of 2961 reports screened, 10 RCTs comprising a total of 789 participants were eligible for inclusion in this meta-analysis. The pooled results using a random-effects model showed that daily ACV intake significantly reduced body weight [SMD: −0.39; 95% CI: −0.63, −0.15; p = 0.001; I² = 62%], BMI [SMD: −0.65; 95% CI: −1.05, −0.26; p = 0.001; I² = 83%], and WC [SMD: −0.34; 95% CI: −0.67, −0.02; p = 0.04; I² = 61%]. However, no significant effects of ACV were observed on the other body composition parameters analyzed. Sensitivity analyses excluding high-risk-of-bias studies confirmed the robustness of ACV’s beneficial effects on body weight and BMI. Subgroup analyses suggested that ACV consumption significantly improved anthropometric parameters when administered for up to 12 weeks, at a dose of 30 mL/day, and in adults who were overweight, obese, or had type 2 diabetes. Conclusions: Overall, this meta-analysis suggests that ACV supplementation may be a promising and accessible adjunctive strategy for short-term weight management in adults with excess body weight or metabolic complications. Full article

(This article belongs to the Topic Functional Foods and Nutraceuticals in Health and Disease)

► Show Figures

Figure 1

11 pages, 710 KB

Open AccessArticle

Effect of 6-Shogaol Derived from Ginger (Zingiber officinale) on Dual-Species Biofilm Formation by Streptococcus mutans and Candida albicans

by Eun-Ha Jung, Geelsu Hwang and Ki-Rim Kim

Nutrients 2025, 17(18), 2999; https://doi.org/10.3390/nu17182999 - 19 Sep 2025

Abstract

Background/Objectives: Dental plaque, a biofilm composed of accumulated oral microorganisms, is a key contributor to various oral diseases. 6-shogaol, a bioactive compound of ginger, is known to have pharmacological activities, including anticancer, anti-inflammatory, and antimicrobial activities. Therefore, we aimed to determine the effects [...] Read more.

Background/Objectives: Dental plaque, a biofilm composed of accumulated oral microorganisms, is a key contributor to various oral diseases. 6-shogaol, a bioactive compound of ginger, is known to have pharmacological activities, including anticancer, anti-inflammatory, and antimicrobial activities. Therefore, we aimed to determine the effects of 6-shogaol on dual-species biofilms of Streptococcus mutans (S. mutans) and Candida albicans (C. albicans). Methods: Dual-species oral biofilms were formed on hydroxyapatite (HA) disks for 42 h and exposed to 6-shogaol. The pH was measured in the experimental medium, and the biomass, colony-forming unit (CFU) of microbial cells, and insoluble extracellular polysaccharides (EPS) were quantified in the biofilm formed on the HA disk. Confocal laser scanning microscopy (CLSM) was used to assess biofilm morphology, and quantitative polymerase chain reaction was performed to analyze gtf gene expression. Results: 6-shogaol dose-dependently reduced insoluble EPS, CFU counts, and dry weight of biofilms. The pH was maintained above 5.5 in the 6-shogaol-treated group. CLSM images showed that S. mutans proliferation, C. albicans hyphal development, and EPS production were markedly inhibited in biofilms treated with 6-shogaol. The expression of gtfB and gtfC was significantly downregulated by 6-shogaol. Conclusions: These findings suggest that 6-shogaol has the potential to be a promising natural product for the prevention and management of oral biofilm-related oral diseases. Full article

(This article belongs to the Section Phytochemicals and Human Health)

► Show Figures

Figure 1

12 pages, 349 KB

Open AccessSystematic Review

Predictors of Successful Weight Restoration in the Treatment of Superior Mesenteric Artery Syndrome: A Systematic Review

by Dennis Gibson, Millie Plotkin, Marina Foster and Philip S. Mehler

Nutrients 2025, 17(18), 2998; https://doi.org/10.3390/nu17182998 - 19 Sep 2025

Abstract

Background/Objectives: Treatment for superior mesenteric artery (SMA) syndrome can include either weight restoration (conservative management) or surgical intervention, with the latter recommended when individuals fail conservative management. However, reasons for failure of conservative management are poorly understood. This systematic review seeks to better [...] Read more.

Background/Objectives: Treatment for superior mesenteric artery (SMA) syndrome can include either weight restoration (conservative management) or surgical intervention, with the latter recommended when individuals fail conservative management. However, reasons for failure of conservative management are poorly understood. This systematic review seeks to better understand predictors of treatment outcomes for malnourished individuals with SMA syndrome, specifically regarding weight restoration and behavioral health intervention, and to better understand why individuals fail conservative management. Methods: Medline, Embase, and Web of Science were searched for articles that assessed treatment interventions for SMA syndrome in malnourished individuals. Results: Seventy-three articles (n = 189 malnourished individuals with SMA syndrome) were included in the final review. Most of the articles (n = 57) had an increased risk of bias as the amount of weight gain with treatment was not explicitly defined and thus the attribution of outcome for “failure” of conservative management could not be ascribed. Modest weight gain (mean 5.64 kg [12.1% body weight increase] or 1.3 kg/m² body mass index increase [9.4% increase in ideal body weight]) was associated with positive outcomes of conservative management. Psychological care also positively impacted treatment outcomes, especially for individuals with comorbid psychiatric conditions. Conclusions: Patients who achieve even modest weight gain have resolution of their SMA-related symptoms without a need for surgical intervention. Psychological treatment should be included for any patient struggling to achieve adequate weight restoration, with subsequent improved outcomes, given the high frequency of comorbid mental health illnesses, especially eating disorders. Full article

(This article belongs to the Section Clinical Nutrition)

► Show Figures

Figure 1

Journal Description

Nutrients

Latest Articles

Journal Menu

Journal Browser

Highly Accessed Articles

Latest Books

E-Mail Alert

News

Topics

Conferences

Special Issues

Topical Collections

Conference Reports

Further Information

Guidelines

MDPI Initiatives

Follow MDPI