Nutrients

13 pages, 690 KB

Open AccessArticle

Effect of Vitamin D Supplementation on Oxidative Stress Biomarkers in Women Following Religious or Intermittent Fasting Patterns

by Spyridon N. Karras, Konstantinos Michalakis, Maria Kypraiou, Marios Anemoulis, Antonios Vlastos, Georgios Tzimagiorgis, Costas Haitoglou, Fotios Tekos, Zoi Skaperda, Periklis Vardakas, Neoklis Georgopoulos, Evangelos G. Papanikolaou and Demetrios Kouretas

Nutrients 2025, 17(21), 3389; https://doi.org/10.3390/nu17213389 (registering DOI) - 28 Oct 2025

Abstract

Background: Vitamin D supplementation may influence oxidative stress, but evidence in populations following specific dietary patterns is limited. Methods: In this non-randomized, two-group exploratory study, 50 Orthodox nuns received vitamin D supplementation (2000 IU/day orally) for 16 weeks, whereas 50 age-matched women following [...] Read more.

Background: Vitamin D supplementation may influence oxidative stress, but evidence in populations following specific dietary patterns is limited. Methods: In this non-randomized, two-group exploratory study, 50 Orthodox nuns received vitamin D supplementation (2000 IU/day orally) for 16 weeks, whereas 50 age-matched women following time-restricted eating (TRE) served as controls receiving no supplementation. Anthropometric parameters, serum 25-hydroxyvitamin D [25(OH)D], and oxidative stress markers—total antioxidant capacity (TAC), glutathione (GSH), and thiobarbituric acid reactive substances—were measured at baseline and post-intervention. Results: At baseline, both groups were comparable in anthropometric and oxidative stress markers, except for serum 25-hydroxyvitamin D [25(OH)D], which was lower in the intervention group. Following supplementation, serum 25(OH)D increased from 15.77 ± 5.21 to 31.24 ± 7.87 ng/mL (p = 0.031) in Orthodox nuns. No significant changes were observed for TAC (0.93 ± 0.11 to 0.97 ± 0.09, p = 0.081) and GSH (6.01 ± 1.55 to 5.81 ± 1.41, p = 0.069), whereas TBARS decreased significantly (7.32 ± 1.31 to 6.94 ± 1.21, p = 0.041). No significant changes were observed in controls under TRE. Changes (Δ) in all variables represented the post–pre difference over the 16-week period. Pearson correlations showed no significant associations between Δ25(OH)D and ΔTAC (r = −0.244, p = 0.346), ΔGSH (r = 0.110, p = 0.675), or ΔTBARS (r = −0.116, p = 0.657). In multivariable regression adjusted for age, weight, body fat percentage, and baseline 25(OH)D, Δ25(OH)D was not an independent predictor of oxidative stress marker changes; however, weight (β = 0.08, p = 0.011) and body fat percentage (β = −0.13, p = 0.014) were associated with reductions in TBARS. Conclusions: In conclusion, sixteen weeks of vitamin D supplementation in women adhering to Orthodox fasting produced no consistent improvements in oxidative stress markers. While a small reduction in TBARS was observed, this effect was modest and appeared indirect, being more closely associated with decreases in body weight and fat mass than with vitamin D status itself. Taken together, our findings indicate an overall neutral impact of vitamin D on redox balance, suggesting that any antioxidant benefit is likely secondary to metabolic or adiposity-related changes. Full article

(This article belongs to the Special Issue The Impact of Food Fortification on Health and Nutrition Outcomes (2nd Edition))

► Show Figures

Figure 1

20 pages, 1360 KB

Open AccessArticle

Clustering of Unhealthy Lifestyle Behaviours and Its Contextual Determinants in Adolescents: A Multilevel Analysis of School-Based Surveys in 45 Countries

by Yohannes Tekalegn Efa, David Roder, Zumin Shi and Ming Li

Nutrients 2025, 17(21), 3388; https://doi.org/10.3390/nu17213388 - 28 Oct 2025

Abstract

Background: This study examined the clustering of unhealthy lifestyle behaviours and their determinants among adolescents across Europe, Central Asia, and North America. Methods: The study included 210,713 adolescents aged 11 to 15 years from 45 countries who participated in the 2018 Health Behaviour [...] Read more.

Background: This study examined the clustering of unhealthy lifestyle behaviours and their determinants among adolescents across Europe, Central Asia, and North America. Methods: The study included 210,713 adolescents aged 11 to 15 years from 45 countries who participated in the 2018 Health Behaviour in School-aged Children (HBSC) study. Lifestyle behaviours, including physical inactivity, inadequate fruit and vegetable consumption, frequent soft drink consumption, alcohol use, and smoking, were used to examine the clustering of unhealthy behaviours. Multilevel mixed-effects logistic regression was employed to assess the associations between unhealthy behaviour clustering (≥3 unhealthy behaviours) and contextual factors at the individual, family, and school levels. Results: A high prevalence of clustered unhealthy behaviours was observed among adolescents, with 51.5% engaging in three or more unhealthy lifestyle behaviours. The odds increased with age (AOR: 1.79, 95% CI: 1.75, 1.84 for those aged ≥ 15 years), among males (AOR: 1.26, 95% CI: 123, 1.28), and among those experiencing higher academic pressure (AOR: 1.13, 95% CI: 1.09, 1.17 for very high academic pressure). In contrast, the odds were lower among adolescents from a higher family affluence background (AOR: 0.62 95% CI: 0.60, 0.65 for high), among adolescents living with both parents (AOR: 0.83, 95 CI: 0.81, 0.85), those reporting higher family support (AOR: 0.62, 95% CI: 0.60, 0.63 for high), higher peer support at school (AOR: 0.87, 95% CI: 0.84, 0.89 for high), and those reporting higher school satisfaction (AOR: 0.50, 95% CI: 0.48, 0.52 for very high). Conclusions: The study reveals that one in two adolescents engages in three or more unhealthy lifestyle behaviours. It emphasises the need to tackle this public health challenge through multisectoral interventions targeting individual-level and contextual factors at the family and school levels. Full article

(This article belongs to the Section Nutrition and Public Health)

19 pages, 852 KB

Open AccessArticle

Probiotic Supplementation Enhances the Effects of a Nutritional Intervention on Quality of Life in Women with Hashimoto’s Thyroiditis—A Double-Blind Randomised Study

by Karolina Osowiecka, Damian Skrypnik and Joanna Myszkowska-Ryciak

Nutrients 2025, 17(21), 3387; https://doi.org/10.3390/nu17213387 - 28 Oct 2025

Abstract

Background: Hashimoto’s thyroiditis (HT) is characterised by chronic inflammation of the thyroid gland. The impact of a health-promoting diet and probiotics on health and quality of life, as well as on the anti-thyroid peroxidase antibody (anti-TPO), is increasingly being researched. However, the [...] Read more.

Background: Hashimoto’s thyroiditis (HT) is characterised by chronic inflammation of the thyroid gland. The impact of a health-promoting diet and probiotics on health and quality of life, as well as on the anti-thyroid peroxidase antibody (anti-TPO), is increasingly being researched. However, the relevance of these factors to the course of HT is yet to be fully established. Objective: The aim of this study was to assess the impact of a 12-week nutritional intervention, comprising a rational, health-promoting diet supplemented with the probiotic strain Lactiplantibacillus plantarum 299v (Lp299v), on eating habits, nutritional status, health and quality of life in patients diagnosed with HT. Methods: The 12-week study involved 64 female patients with HT, divided into two groups: the NE+Lp299v group, which received nutritional education and Lp299v (n = 32); and the NE+placebo group, which received nutritional education and placebo (n = 32). Before and after the intervention, anthropometric parameters, body composition analysis, blood pressure, blood anti-TPO levels, dietary habits, quality of life, and gastrointestinal symptoms were assessed. Results: The NE+Lp299v intervention improved overall quality of life (60.94 pts. vs. 35.94 pts.), including 12 of 14 domains, and the diet quality index (11.03 pts. vs. 18.50 pts.). The NE+placebo group improved overall quality of life (54.69 pts. vs. 39.84 pts.), including 3 of 14 domains, and the diet quality index (12.34 pts. vs. 19.18 pts.). Anti-TPO blood levels and body mass index did not improve in either group. Conclusions: Lp299v can enhance the efficacy of nutritional education in improving the quality of life of individuals diagnosed with HT. However, these benefits appear to be independent of anti-TPO levels. Full article

(This article belongs to the Section Nutrition in Women)

► Show Figures

Figure 1

26 pages, 8137 KB

Open AccessArticle

Natural Compound Melatonin Suppresses Breast Cancer Development by Regulating Circadian Rhythm

by Yuanli He, Chenchen Hu, Feiming Hu, Yuanjie Sun, Lin Guo, Junyi Ren, Chenying Han, Yuhui Li, Xiyang Zhang, Yubo Sun, Junqi Zhang, Sirui Cai, Yueyue Wang, Dongbo Jiang, Kun Yang and Shuya Yang

Nutrients 2025, 17(21), 3386; https://doi.org/10.3390/nu17213386 - 28 Oct 2025

Abstract

Background: Breast cancer remains a major global health threat to women. While current therapies exist, their limitations necessitate novel strategies. Melatonin, an endogenous circadian regulator, has shown anti-tumor potential, but its mechanisms from a circadian perspective require further exploration. Methods: The anti-tumor effects [...] Read more.

Background: Breast cancer remains a major global health threat to women. While current therapies exist, their limitations necessitate novel strategies. Melatonin, an endogenous circadian regulator, has shown anti-tumor potential, but its mechanisms from a circadian perspective require further exploration. Methods: The anti-tumor effects of melatonin were evaluated through cell proliferation, colony formation, and apoptosis assays. Through data analysis and experimental verification at the RNA and protein levels, the regulatory effect of it on the core clock gene BMAL1 was studied. The role of BMAL1 in mediating melatonin’s suppression of glucose metabolism was assessed by measuring glucose uptake and lactate production. Downstream effector molecules of BMAL1 were identified through molecular interaction and transcriptional regulation analyses. Results: Melatonin significantly inhibited breast cancer cell proliferation and colony formation and induced apoptosis. Mechanistically, it upregulates the core clock gene BMAL1, which suppresses glucose metabolism. ALDH3A1 was identified as a key downstream target of BMAL1, defining a novel “melatonin-BMAL1-ALDH3A1” axis. In vivo studies confirmed that this axis effectively inhibits tumor growth without apparent toxicity, and SR8278 also shows a synergistic effect when used in combination with melatonin. Conclusions: Our findings elucidate the role of the “melatonin-BMAL1-ALDH3A1” axis in combating breast cancer, offering a new direction for treatment and laying the groundwork for developing precision chronotherapy-based combination regimens. Full article

(This article belongs to the Section Phytochemicals and Human Health)

► Show Figures

Graphical abstract

19 pages, 674 KB

Open AccessReview

Nutritional Strategies for Olympic Biathletes: A Practical Review

by Mateusz Gawelczyk, Magdalena Kaszuba and Miroslav Petr

Nutrients 2025, 17(21), 3385; https://doi.org/10.3390/nu17213385 - 28 Oct 2025

Abstract

Biathlon is a winter Olympic sport that combines high-intensity cross-country skiing with precise rifle shooting. These dual demands require athletes to develop exceptional aerobic capacity while maintaining fine motor accuracy under physiological stress. Despite its complexity, nutritional strategies in biathlon remain under-explored and [...] Read more.

Biathlon is a winter Olympic sport that combines high-intensity cross-country skiing with precise rifle shooting. These dual demands require athletes to develop exceptional aerobic capacity while maintaining fine motor accuracy under physiological stress. Despite its complexity, nutritional strategies in biathlon remain under-explored and recommendations are often extrapolated from related endurance sports. This narrative review aims to summarise existing knowledge on nutrition in biathlon, highlight sport-specific challenges and identify areas for future research. The main findings indicate that biathletes face very high energy demands, with daily expenditure exceeding 7000 kcal during intensive training. Carbohydrates are the primary fuel source, with intake recommendations based on training intensity and duration (6–12 g/kg/d). Furthermore, protein is essential for muscle repair, recovery and adaptation. To achieve the recommended intake of 1.6 g/kg/day, it is advisable to consume meals containing approximately 0.3 g/kg of high-quality protein every three to four hours. Given the frequency of training sessions, effective recovery strategies are important in biathlon. When recovery is a priority, biathletes should consume a meal comprising protein (approximately 0.3 g/kg) and carbohydrates (approximately 1.2 g/kg) before key training sessions. Micronutrient and vitamin deficiencies are not commonly observed in biathletes due to their high calorie intake. However, concerns regarding iron and vitamin D are common among endurance athletes due to the high risk of low energy intake, diets lacking in iron, and insufficient exposure to sunlight. On the day of the race, it is recommended that biathletes plan their meals to ensure that they meet their nutritional needs and begin recovery as soon as possible after the race is over. Biathletes may use specific supplements to enhance performance and health during preparation and competition. However, it is important to note that some supplements that improve performance may harm shooting accuracy. Current guidance is provisional, and future research should adopt a dual-performance framework that evaluates both endurance output and shooting precision under realistic competition conditions. Full article

(This article belongs to the Special Issue The Effects of Targeted Nutritional Strategies on Athlete Performance)

23 pages, 813 KB

Open AccessSystematic Review

Human Milk Fortification and Necrotizing Enterocolitis in Very Low Birthweight Infants: State of Evidence and Systematic Review with Meta-Analysis

by Sarah M. Reyes, Tristen L. Paul and Jenelle Ferry

Nutrients 2025, 17(21), 3384; https://doi.org/10.3390/nu17213384 - 28 Oct 2025

Abstract

Background: Necrotizing enterocolitis (NEC) remains a leading cause of morbidity and mortality in very low birthweight (VLBW) infants. Human milk feeding and standardized feeding protocols are protective, but clinical practice varies, particularly in fortifier choice. Whether human milk-derived fortifiers reduce NEC risk compared [...] Read more.

Background: Necrotizing enterocolitis (NEC) remains a leading cause of morbidity and mortality in very low birthweight (VLBW) infants. Human milk feeding and standardized feeding protocols are protective, but clinical practice varies, particularly in fortifier choice. Whether human milk-derived fortifiers reduce NEC risk compared with cow milk-derived fortifiers remains unclear. Methods: We conducted a systematic state-of-evidence review and meta-analysis, searching PubMed, Web of Science, and Scopus through July 2025. Eligible studies included RCTs and observational cohorts of VLBW infants comparing an exclusive human milk diet (EHMD) including human milk-derived fortifiers to cow milk-derived diets. Two reviewers independently screened and extracted data. Both RCTs and observational studies were included to evaluate consistency of effect estimates across designs and to account for heterogeneity in control group feeding practices. Pooled odds ratios (ORs) with 95% CIs were calculated using a Sidik–Jonkman random-effects model. Sensitivity analyses by study design and exclusion of infant formula from controls were performed. Results: Twenty studies (five RCTs, 15 observational; N = 6794 infants) met inclusion criteria, most enrolling infants born ≤1250 g. Compared with cow milk-containing diets, EHMD was associated with lower odds of Bell Stage ≥ 2 NEC (OR: 0.59; 95% CI: 0.42, 0.81; p < 0.001; n = 4625) and surgical NEC (OR: 0.43; 95% CI: 0.32, 0.58; p < 0.0001; n = 4754). In direct comparisons of fortifier type with a base diet of human milk, estimates suggested lower odds of Bell Stage ≥ 2 NEC by 35% (OR: 0.65; 95% CI: 0.44, 0.97; p = 0.03, n = 2102) and surgical NEC by 49% (OR: 0.51; 95% CI: 0.26, 0.98; p = 0.04; n = 1659) with human milk-derived fortifiers. Effect estimates were generally consistent across study designs, although precision and statistical significance varied. Conclusions: EHMD with human milk-derived fortifiers was associated with lower odds of medical and surgical NEC in VLBW infants, with most evidence from infants born ≤1250 g, reflecting current clinical use in the highest-risk population. Although the number and sample sizes of RCTs remain limited, the consistency of effect estimates across both RCTs and observational studies, together with significance of pooled analyses, strengthens confidence in these findings. Pragmatic and registry-based studies using standardized fortification protocols may provide the most efficient pathway to strengthen the evidence base. Full article

(This article belongs to the Special Issue Nutrition Management in Neonatal Health)

18 pages, 916 KB

Open AccessArticle

Effect of Oral and Topical Sodium Bicarbonate on Functional Recovery and Soccer-Specific Performance After Exercise-Induced Muscle Damage: A Randomized, Double-Blind, Placebo-Controlled Study

by William H. Gurton, Lewis A. Gough, Anthony Lynn and Mayur K. Ranchordas

Nutrients 2025, 17(21), 3383; https://doi.org/10.3390/nu17213383 - 28 Oct 2025

Abstract

Objectives: This study assessed the influence of oral and topical sodium bicarbonate (SB) on recovery and soccer-specific performance after exercise-induced muscle damage (EIMD). Methods: In a randomized, double-blind, placebo-controlled, parallel group design, 24 soccer players were allocated to oral SB, topical [...] Read more.

Objectives: This study assessed the influence of oral and topical sodium bicarbonate (SB) on recovery and soccer-specific performance after exercise-induced muscle damage (EIMD). Methods: In a randomized, double-blind, placebo-controlled, parallel group design, 24 soccer players were allocated to oral SB, topical SB (PR Lotion, Momentous), or placebo (PLA) (n = 8 per condition) and attended six laboratory sessions: (i) familiarization, (ii) baseline measures, and (iii) four experimental trials on consecutive days. Muscle damage was induced on day 1 using 40 × 15 m sprints, after which either 0.3 g·kg⁻¹ body mass (BM) SB (SB-ORAL), 0.9036 g·kg⁻¹ BM PR Lotion (SB-LOTION), or an equivalent PLA was given. Recovery outcomes were measured post-EIMD, 1 d, 2 d, and 3 d post (day 1–4). Soccer-specific performance was repeated 3 d post, with supplements administered again 2 h pre-exercise. Recovery measures included muscle soreness, vertical jump height, and maximal voluntary isometric contraction. Illinois agility test (IAT), 8 × 25 m repeated sprints, and Yo-Yo Intermittent Recovery Test Level 2 (Yo-Yo IR2) were assessed as soccer-specific performance. Results: Neither SB-ORAL nor SB-LOTION accelerated indices of recovery but decline in soccer-specific performance from baseline to 3 d post was attenuated for SB-ORAL, with significant effects for IAT (p = 0.032, g = 1.69) and Yo-Yo IR2 (p = 0.026, g = 1.61) compared with PLA. Conclusions: SB did not accelerate recovery following EIMD but prescribing oral SB before subsequent exercise might rescue key performance indicators. These findings offer implications for practitioners working with soccer players during periods where full recovery is not achieved. Full article

(This article belongs to the Special Issue Nutritional Supplements and Diet in Athletic Performance)

32 pages, 7004 KB

Open AccessReview

Phytochemical Insights and Industrial Applications of Camellia japonica Leaves: A Focus on Sustainable Utilization

by Ezgi Nur Yuksek, Miguel A. Prieto and Antia G. Pereira

Nutrients 2025, 17(21), 3382; https://doi.org/10.3390/nu17213382 - 28 Oct 2025

Abstract

In response to the increasing interest in medicinal plants, researchers have called for the investigation of underexplored, widely distributed species, such as those within the Camellia genus. Camellia japonica L., though not native to Galicia (NW Spain), is widely cultivated there, primarily for [...] Read more.

In response to the increasing interest in medicinal plants, researchers have called for the investigation of underexplored, widely distributed species, such as those within the Camellia genus. Camellia japonica L., though not native to Galicia (NW Spain), is widely cultivated there, primarily for ornamental purposes. Recent phytochemical analyses of C. japonica leaves have identified a variety of bioactive compounds, with phenolic compounds being the most abundant, along with carotenoids, terpenoids and fatty acids. These molecules exhibit a range of biological activities, including antioxidant, antimicrobial, anti-inflammatory, and anticancer effects. Nonetheless, certain constituents, such as saponins, triterpenes, and tannins, may exhibit anti-nutritional or mild toxic effects under specific conditions. This review specifically examines the bioactive compounds found in C. japonica leaves and their associated health benefits. Furthermore, it underscores the need for innovative approaches to develop sustainable industrial processes for utilizing C. japonica leaves, with potential applications in the food, pharmaceutical, and cosmetic industries. Full article

(This article belongs to the Section Phytochemicals and Human Health)

17 pages, 677 KB

Open AccessArticle

Serum Lipid Profile in Polish Women Adhering to Different Dietary Patterns: The Cardioprotective Potential of Plant-Based Diets

by Patrycja Gogga, Patrycja Szulc and Agata Janczy

Nutrients 2025, 17(21), 3381; https://doi.org/10.3390/nu17213381 - 28 Oct 2025

Abstract

Background/Objectives: Cardiovascular diseases (CVDs) remain the leading cause of mortality worldwide, with elevated low-density lipoprotein cholesterol (LDL-C) and total cholesterol (TC) being major risk factors. Diet is a key modulator of these parameters, and healthful plant-based diets—popular particularly among women—are associated with cardiovascular [...] Read more.

Background/Objectives: Cardiovascular diseases (CVDs) remain the leading cause of mortality worldwide, with elevated low-density lipoprotein cholesterol (LDL-C) and total cholesterol (TC) being major risk factors. Diet is a key modulator of these parameters, and healthful plant-based diets—popular particularly among women—are associated with cardiovascular benefits. The present study aimed to evaluate the serum lipid profile and to identify dietary components associated with differences in lipid fractions in healthy women adhering to different dietary patterns. Methods: This was a cross-sectional, single-center, convenience sample study of 128 healthy women of similar age, normal BMI, and comparable body fat, allocated to four dietary groups: vegans (n = 45), lacto-ovo-vegetarians (n = 52), pescatarians (n = 12), and omnivores (n = 19). Serum lipid profiles were determined using enzymatic kits. Intake of selected nutrients was assessed based on 7-day dietary records. Physical activity was estimated using the physical activity level (PAL) index. Analyses included different ANOVA approaches and PCA. Results: Omnivores exhibited the highest serum concentrations of LDL-C, TC, and TGs compared with other dietary groups. A significant association was noted between elevated TC and higher intake of saturated fatty acids (SFAs), cholesterol, and animal protein, accompanied by lower intake of fiber and plant protein. Additionally, women with lower TGs and higher HDL-C showed lower PAL values. No significant differences in HDL-C concentrations were observed between groups. Conclusions: Plant-based diets, defined by lower consumption of SFAs, cholesterol, and animal protein alongside higher intake of fiber and plant protein, were associated with a more favorable lipid profile. These findings support the role of vegetarian and vegan diets in CVDs prevention and management, particularly when coupled with regular physical activity; however, further interventional studies among diverse populations are necessary to confirm our results. Full article

(This article belongs to the Special Issue Plant-Based Diet: Health Perspective)

► Show Figures

Figure 1

17 pages, 1358 KB

Open AccessArticle

Gut Microbiota, Intestinal Barrier Function, and Metabolism Across Adiposity and Glucose Tolerance

by Karynne Grutter Lopes, Maria das Graças Coelho de Souza, Fernanda de Azevedo Marques Lopes, Vicente Lopes da Silva Júnior, Ana Teresa Pugas Carvalho, Davy Carlos Mendes Rapozo, Carolina Monteiro de Lemos Barbosa, Eliete Bouskela, Raquel Carvalho Castiglione, Rodolpho Matos Albano and Luiz Guilherme Kraemer-Aguiar

Nutrients 2025, 17(21), 3380; https://doi.org/10.3390/nu17213380 - 28 Oct 2025

Abstract

Background/Objectives: Obesity and dysglycemia are increasingly associated with intestinal barrier dysfunction and alterations in gut microbiota. Intestinal hyperpermeability is emerging as a therapeutic target in metabolic disorders, but human data integrating barrier biomarkers, epithelial morphology, and microbial composition remain scarce. Methods: Forty-six adults [...] Read more.

Background/Objectives: Obesity and dysglycemia are increasingly associated with intestinal barrier dysfunction and alterations in gut microbiota. Intestinal hyperpermeability is emerging as a therapeutic target in metabolic disorders, but human data integrating barrier biomarkers, epithelial morphology, and microbial composition remain scarce. Methods: Forty-six adults (82.6% female; 38.3 ± 7.8 years) were stratified into lean normoglycemic controls (CON), individuals with obesity and normoglycemia (NOB), and those with obesity and dysglycemia (DOB). Biochemical/inflammatory biomarkers, such as lipopolysaccharide (LPS) and LPS-binding protein (LBP), were measured. Duodenal biopsies were obtained by upper digestive videoendoscopy. Histomorphometry, expression of junctional and cytoskeletal proteins, and enzymatic activity of the duodenal epithelium were used as markers of intestinal permeability. Fecal microbiota composition (FMC) was analyzed by amplifying the V4 region of the 16S rRNA gene, which was sequenced using next-generation sequencing technology. Results: Duodenal histomorphometry did not differ across groups. Intestinal alkaline phosphatase (IAP) was significantly lower in DOB compared to CON. LPS correlated positively with fat mass, and LBP with the waist-to-hip ratio. The villus-to-crypt ratio correlated negatively with BMI, while IAP correlated inversely with fasting glucose and HbA1c. β-actin expression was inversely associated with BMI, glucose, insulin, and HOMA-IR. Microbiota diversity indices were similar between groups, although specific taxa, particularly within the Clostridiales order, were reduced in dysglycemia. Conclusions: Reduced IAP activity and consistent correlations between barrier biomarkers and metabolic parameters highlight intestinal barrier dysfunction as a relevant feature of obesity and dysglycemia. Subtle microbiota alterations further support a link between gut ecology and metabolic control. These findings underscore the intestinal barrier as a promising therapeutic target in metabolic disorders. Full article

(This article belongs to the Special Issue Dietary Patterns and Gut Microbiota)

► Show Figures

Figure 1

13 pages, 618 KB

Open AccessArticle

The Relationship Between Disease Activity and Fecal Calprotectin and Fecal Occult Blood in Inflammatory Bowel Disease: The Role of Nutritional Status

by Ali Bilgen and Hale Akpınar

Nutrients 2025, 17(21), 3379; https://doi.org/10.3390/nu17213379 - 28 Oct 2025

Abstract

Background: Inflammatory bowel disease (IBD), encompassing ulcerative colitis (UC) and Crohn’s disease (CD), is characterized by chronic intestinal inflammation with fluctuating clinical severity. Although fecal calprotectin (FC) and fecal occult blood (FOBT) are established noninvasive biomarkers of intestinal inflammation, their interplay with nutritional [...] Read more.

Background: Inflammatory bowel disease (IBD), encompassing ulcerative colitis (UC) and Crohn’s disease (CD), is characterized by chronic intestinal inflammation with fluctuating clinical severity. Although fecal calprotectin (FC) and fecal occult blood (FOBT) are established noninvasive biomarkers of intestinal inflammation, their interplay with nutritional status and disease activity has not been fully elucidated. This study aimed to explore the relationship between FC, FOBT, and disease activity in IBD, and to assess the potential mediating role of nutritional status as measured by the prognostic nutritional index (PNI). Methods: This retrospective study includes 128 adult patients with confirmed IBD (50 UC and 78 CD) examined at a tertiary care center between December 2023 and August 2025. Disease activity was assessed using the Mayo score for UC and the Harvey–Bradshaw Index for CD. FC levels were quantitatively measured using an enzyme-linked immunosorbent assay (ELISA), and fecal occult blood testing was performed with an automated latex agglutination-based system. Multivariable linear regression models were conducted to identify independent predictors of disease activity. Results: UC patients had significantly higher FC levels (278.0 vs. 133.5 µg/g, p < 0.001), FOBT positivity rates (76.7% vs. 43.6%, p = 0.002), and lower PNI (49.2 ± 4.2 vs. 51.5 ± 4.6, p = 0.048) compared to CD patients. In both UC and CD, disease activity scores were positively correlated with FC, FOBT positivity, CRP, and duration of illness, and negatively correlated with PNI (p < 0.05). In multivariable regression, PNI lost predictive value when FC and FOBT were included; FC and FOBT remained strong independent predictors of disease activity. Conclusions: FC and fecal occult blood are independently associated with higher disease activity in IBD, and may mediate the observed relationship between poor nutritional status and inflammation severity. The loss of significance of PNI in adjusted models suggests that intestinal inflammation and bleeding may act as intermediaries linking malnutrition to disease activity. Full article

(This article belongs to the Section Clinical Nutrition)

► Show Figures

Figure 1

12 pages, 1080 KB

Open AccessArticle

Prevalence and Impact of Zinc Deficiency on Clinical Outcomes in Inflammatory Bowel Disease

by Hend Almuhaya, Raghad Alsalamah, Asma Sallam, Amgad Alonazy, Atheer AlAwwad, Gamal Mohamed, Abdulelah Almutairdi, Mashary Attamimi and Badr Al-Bawardy

Nutrients 2025, 17(21), 3378; https://doi.org/10.3390/nu17213378 - 28 Oct 2025

Abstract

Background: Inflammatory bowel disease (IBD) can lead to zinc deficiency, which plays a critical role in immune function and tissue repair. This study aims to determine the prevalence, clinical characteristics, and impact of zinc deficiency in IBD patients. Methods: This is a retrospective [...] Read more.

Background: Inflammatory bowel disease (IBD) can lead to zinc deficiency, which plays a critical role in immune function and tissue repair. This study aims to determine the prevalence, clinical characteristics, and impact of zinc deficiency in IBD patients. Methods: This is a retrospective study of patients aged ≥14 years with confirmed IBD and available zinc level measurement. Zinc deficiency was defined as a level <10.6 µmol/L. Primary outcomes included the prevalence of zinc deficiency and the characterization of the clinical profile of patients with zinc deficiency. Secondary outcomes included IBD-related hospitalizations, emergency room visits, surgeries, and complications (anemia, small bowel obstruction, new perianal disease, intra-abdominal abscess). Results: Among 447 patients (54.4% male; 79.2% Crohn’s disease) with a median age of 29 years (IQR: 22–38), 45.6% had zinc deficiency (83.8% Crohn’s disease). Zinc-deficient patients had higher C-reactive protein and fecal calprotectin (both p < 0.001) levels, and were more likely to be on corticosteroids (p = 0.04). Zinc deficiency was associated with higher rates of IBD-related hospitalizations (48.0% vs. 17.7%), surgeries (19.6% vs. 5.8%), complications (30.4% vs. 12.4%), and emergency room visits (40.2% vs. 17.3%) (all p < 0.001). Upon multivariate analysis, predictors of IBD-related hospitalization were zinc deficiency (OR 2.42, 95% CI 1.07–5.48, p = 0.03) and low albumin (OR 9.03, 95% CI 3.38–24.15, p < 0.001). Zinc deficiency was associated with IBD-related surgeries (OR 5.23, 95% CI 1.27–21.45, p = 0.02) and complications (OR 3.98, 95% CI 1.52–10.41, p = 0.005). Conclusions: Zinc deficiency is prevalent in patients with IBD, associated with a high inflammatory burden, and was linked to worse clinical outcomes after controlling for markers of inflammation. Full article

(This article belongs to the Special Issue Impact of Nutritional Approaches on Inflammatory Bowel Disease Patients)

► Show Figures

Figure 1

15 pages, 383 KB

Open AccessArticle

Intergenerational Transmission of Proactive Health Behaviors Among Adolescents with Overweight or Obesity: The Mediating Role of Self-Efficacy and Family Cohesion

by Tian Hu, Jingwei Zhou, Lianlong Yu, Suyun Li, Qian Ning Leong, Jingjing Li, Yunping Zhou and Ying Jiang

Nutrients 2025, 17(21), 3377; https://doi.org/10.3390/nu17213377 - 28 Oct 2025

Abstract

Background/Objectives: The family environment exerts a significant influence on the development of weight-related behaviors among adolescents. However, the lack of motivation often leads to failure in sustaining health behaviors, particularly among adolescents with overweight and obesity. This study examined whether parental healthy behaviors [...] Read more.

Background/Objectives: The family environment exerts a significant influence on the development of weight-related behaviors among adolescents. However, the lack of motivation often leads to failure in sustaining health behaviors, particularly among adolescents with overweight and obesity. This study examined whether parental healthy behaviors are associated with proactive health behaviors among adolescents with overweight and obesity and investigated the mediating roles of adolescents’ self-efficacy and family cohesion in these relationships within Chinese families. Methods: We recruited 4932 adolescents with overweight and obesity aged 10 to 15 in Shandong Province between October and December 2024. Participants completed validated questionnaires assessing parental healthy behaviors, self-efficacy, family cohesion, and proactive health behaviors. We analyzed the data using multilevel regression models and mediation analysis, controlling for key sociodemographic factors. Subgroup analyses were conducted by gender and region level. Results: Both fathers’ (β = 0.442, 95% CI = 0.263, 0.620) and mothers’ (β = 0.525, 95% CI = 0.336, 0.714) healthy behaviors were positively associated with proactive health behaviors among adolescents with overweight and obesity in the adjusted model. Further mediation analysis revealed that adolescent self-efficacy and family cohesion significantly mediated these relationships. Conclusions: This work suggested that parental healthy behaviors were positively associated with proactive health behaviors among adolescents with overweight and obesity. Additionally, higher self-efficacy and family cohesion enhance these associations. The findings offered evidence-based recommendations for creating family-centered interventions targeting adolescents with overweight and obesity. Future research should employ longitudinal designs to understand these relationships better and improve the efficacy of family-based interventions for adolescents with overweight and obesity. Full article

(This article belongs to the Section Nutrition and Obesity)

► Show Figures

Figure 1

17 pages, 900 KB

Open AccessReview

A Potential Role of Natural Bioactive Compounds Found in Food in the Prevention of Idiopathic Parkinson’s Disease

by Sandro Huenchuguala and Juan Segura-Aguilar

Nutrients 2025, 17(21), 3376; https://doi.org/10.3390/nu17213376 - 28 Oct 2025

Abstract

Various clinical studies aimed at modifying the progression of idiopathic Parkinson’s disease have been unsuccessful. Similarly, several nutritional trials using bioactive compounds have shown positive effects for patients but have also failed to slow or reduce the disease’s progression. This repeated failure is [...] Read more.

Various clinical studies aimed at modifying the progression of idiopathic Parkinson’s disease have been unsuccessful. Similarly, several nutritional trials using bioactive compounds have shown positive effects for patients but have also failed to slow or reduce the disease’s progression. This repeated failure is likely because these studies ignore the extremely slow neurodegenerative process, which unfolds over many years. The molecular mechanism behind the loss of neuromelanin-containing dopaminergic neurons in the nigrostriatal system in idiopathic Parkinson’s disease remains unclear. This is a conceptual/theoretical review based mainly on mechanistic and preclinical evidence, with no direct clinical data. However, research suggests that aminochrome, an endogenous neurotoxin, may trigger the degeneration of these neurons through a single-neuron degeneration model. In this model, aminochrome selectively destroys individual neurons without spreading to neighboring cells. Aminochrome is produced during neuromelanin synthesis, a process that is normally harmless because protective enzymes like DT-diaphorase and glutathione transferase M2-2 neutralize aminochrome’s neurotoxic effects. Increasing the levels of these enzymes could offer neuroprotection. The KEAP1/NRF2 signaling pathway is critical for regulating antioxidant enzymes, such as DT-diaphorase and glutathione transferase M2-2. Importantly, specific bioactive compounds from food can activate this pathway, increasing the production of these protective enzymes. For instance, the omega-3 fatty acids eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), along with astaxanthin—a compound present in cold-water fish like salmon—have been demonstrated to enhance enzyme expression. This connection leads to a compelling question: Could dietary interventions help prevent idiopathic Parkinson’s disease? Answering this will require further research. Full article

(This article belongs to the Special Issue Bioactive Compounds and Functional Foods in Human Health—2nd Edition)

► Show Figures

Figure 1

27 pages, 1755 KB

Open AccessReview

Zinc as a Modulator of miRNA Signaling in Obesity

by Nurpudji Astuti Taslim, Anne Maria Graciela, Dante Saksono Harbuwono, Andi Yasmin Syauki, Andrew Nehemia Anthony, Nur Ashari, Andi Makbul Aman, Raymond Rubianto Tjandrawinata, Hardinsyah Hardinsyah, Agussalim Bukhari and Fahrul Nurkolis

Nutrients 2025, 17(21), 3375; https://doi.org/10.3390/nu17213375 - 28 Oct 2025

Abstract

Background: Obesity is a multifactorial metabolic disorder influenced not only by excessive caloric intake but also by micronutrient imbalances such as zinc deficiency. Emerging evidence suggests that zinc regulates microRNA (miRNA) biogenesis and expression, linking nutritional status to metabolic regulation. Objective: [...] Read more.

Background: Obesity is a multifactorial metabolic disorder influenced not only by excessive caloric intake but also by micronutrient imbalances such as zinc deficiency. Emerging evidence suggests that zinc regulates microRNA (miRNA) biogenesis and expression, linking nutritional status to metabolic regulation. Objective: This review delineates the molecular interplay between zinc and miRNAs in obesity, emphasizing the mechanistic, clinical, and translational relevance of zinc-sensitive miRNAs in adipogenesis, insulin resistance, inflammation, and oxidative stress. Results: Zinc deficiency alters miRNA expression profiles associated with metabolic dysregulation. Key miRNAs—miR-21, miR-34a, miR-122, and miR-144-3p—are consistently modulated by zinc status, influencing inflammation, lipid metabolism, and insulin signaling. Zinc repletion restores several miRNAs (e.g., miR-10b, miR-155, miR-145), suggesting reversibility, while excessive zinc may upregulate miR-144-3p and exacerbate oxidative stress. Circulating and exosomal miRNAs show promise as dynamic biomarkers for zinc intervention efficacy. Methods: A literature search was performed in 4 databases up to August 2025 using keywords related to zinc, miRNAs, and obesity. Eligible studies included both preclinical and human research evaluating zinc status or supplementation and miRNA expression in metabolic contexts. Conclusion: Maintaining optimal zinc levels may normalize miRNA expression and improve insulin sensitivity. The “zinc–miRNA axis” represents a novel frontier for precision nutrition in obesity management. Full article

(This article belongs to the Special Issue Nutrition for the Prevention and Control of Chronic Degenerative Diseases)

► Show Figures

Graphical abstract

17 pages, 770 KB

Open AccessArticle

Voluntary Food Reformulation Initiatives Failed to Reduce the Salt Content of Artisanal Breads in Greece

by Georgios Marakis, Sotiria Kotopoulou, Stavroula Skoulika, Georgios Petropoulos, Zoe Mousia, Emmanuella Magriplis and Antonis Zampelas

Nutrients 2025, 17(21), 3374; https://doi.org/10.3390/nu17213374 - 27 Oct 2025

Abstract

Background: Reducing salt in bread is considered a straightforward, cost-effective public health intervention and is implemented in several countries, either voluntarily or through legislation. A Memorandum of Understanding (MoU) was signed in Greece in 2016, setting a voluntary maximum salt content of 1.2% [...] Read more.

Background: Reducing salt in bread is considered a straightforward, cost-effective public health intervention and is implemented in several countries, either voluntarily or through legislation. A Memorandum of Understanding (MoU) was signed in Greece in 2016, setting a voluntary maximum salt content of 1.2% in artisanal bread. This study aimed to evaluate the effectiveness of the MoU and assessed the potential impact of reducing salt in bread on overall salt intake, using the MoU target and the relevant WHO global sodium benchmark. Methods: Artisanal bread samples (n = 253) randomly collected from different parts of Greece in 2024 were analyzed for salt content and compared with samples collected in 2012 (n = 220). Salt intake from bread was estimated using data from the Hellenic National Nutrition and Health Survey (HNNHS), and modeling scenarios were conducted. Results: The MoU and related voluntary awareness activities were ineffective as a strategy to reduce salt in bread. The mean salt content in bread in 2024 was 1.41 (0.30)%, representing a 6.8% increase compared to 1.32 (0.31)% in 2012. Only 19.4% of samples in 2024 contained ≤1.2% salt, compared to 31.8% in 2012. Full MoU compliance would enable an additional 3.1% of Greek bread consumers, currently exceeding 5 g in their daily salt intake from foods alone, to reduce their intake to below 5 g. This would rise to 6.2% if the WHO sodium benchmark was implemented. Conclusions: A mandatory salt limit, aligned with the WHO global benchmark, is urgently needed to support national reformulation strategies. This work can contribute to European and international discussions on food reformulation. Full article

(This article belongs to the Special Issue Towards Healthy and Sustainable Diets: Environmental and Nutritional Perspectives)

► Show Figures

Graphical abstract

20 pages, 878 KB

Open AccessArticle

Functional Properties of Enriched Curd with Collagen and Plant Phytochemicals for Athletes and Physiological Benefits: Evidence Data from Preclinical Trials In Vivo

by Klara Zharykbasova, Aitbek Kakimov, Yerlan Zharykbasov, Zhainagul Kakimova, Raimkhanova Guldana, Kozykenova Zhanna, Beisembayeva Galiya, Zhanat Baigazinov, Tibor Kovács and Amin Shahrokhi

Nutrients 2025, 17(21), 3373; https://doi.org/10.3390/nu17213373 - 27 Oct 2025

Abstract

Background/Objectives: The aim of this study was to establish the multifactorial physiological effect of a functional curd product enriched with collagen-containing concentrate and phytochemical extracts of various natures, under conditions of in vivo experiment. Methods: Biomarkers, such as antioxidant activity (glutathione peroxidase, glutathione [...] Read more.

Background/Objectives: The aim of this study was to establish the multifactorial physiological effect of a functional curd product enriched with collagen-containing concentrate and phytochemical extracts of various natures, under conditions of in vivo experiment. Methods: Biomarkers, such as antioxidant activity (glutathione peroxidase, glutathione reductase, MDA), immune response (IgA, IgG, IgM, IL-6, TNF-α), and purine metabolism (uric acid, xanthine oxidase, 5′-nucleotidase) were selected for evaluation and their influence change. The model was white outbred rats (n = 45), randomly distributed into three groups: control (basic product), experimental group 1 (supplements of collagen-containing concentrate and extract of the composition of sea buckthorn and rosehips), and experimental group 2 (supplements of collagen-containing concentrate and extract of the composition of yarrow and sage). Results: In both experimental groups, a reliable increase in the enzymatic activity of the antioxidant system, a decrease in lipid peroxidation and the level of proinflammatory cytokines, an increase in immunoglobulins, and activation of 5′-nucleotidase were observed. The most pronounced effects were observed with the introduction of a curd product containing collagen-containing concentrate and sea buckthorn and rosehip extract. Conclusions: The scientific novelty of the study lies in the first comprehensive in vivo evaluation of the combined enrichment of a dairy product with collagen and plant extracts for a set of biomarkers. The data obtained confirm the physiological activity and functional properties of the developed product, which can be considered as a promising means of specialized and sports nutrition with proven biological action. Full article

(This article belongs to the Section Sports Nutrition)

16 pages, 536 KB

Open AccessArticle

Dietary Omega-3 Fatty Acids from Fish and Risk of Metabolic Dysfunction-Associated Steatotic Liver Disease in a Mediterranean Population: Findings from the NUTRIHEP Cohort

by Rossella Tatoli, Bonfiglio Caterina, Rossella Donghia, Pasqua Letizia Pesole, Luigi Fontana and Gianluigi Giannelli

Nutrients 2025, 17(21), 3372; https://doi.org/10.3390/nu17213372 - 27 Oct 2025

Abstract

Background: Metabolic Dysfunction-Associated Steatotic Liver Disease (MASLD) is linked to metabolic syndrome, obesity, and type 2 diabetes. Omega-3 fatty acids, especially EPA and DHA from fish, may protect against hepatic steatosis. Methods: From 2015 to 2018, all participants were invited to [...] Read more.

Background: Metabolic Dysfunction-Associated Steatotic Liver Disease (MASLD) is linked to metabolic syndrome, obesity, and type 2 diabetes. Omega-3 fatty acids, especially EPA and DHA from fish, may protect against hepatic steatosis. Methods: From 2015 to 2018, all participants were invited to the first follow-up, where 1426 (62% response rate) responded and underwent the same standardized protocol as at baseline. For this analysis, the study is a cross-sectional investigation focusing solely on follow-up data, which included 1297 adults. MASLD was diagnosed via standardized ultrasound, and dietary intake was assessed using the validated EPIC Food Frequency Questionnaire. Associations between total EPA and DHA intake and MASLD were examined using logistic regression models adjusted for age, sex, marital status, occupation, income, Mediterranean diet adherence, liver enzymes, and C-reactive protein. Results: Overall, 48.5% of participants had MASLD. Higher total EPA and DHA intake was associated with lower odds of MASLD (EPA highest vs. lowest quartile: OR = 0.572, 95% CI 0.400–0.818; DHA highest vs. lowest quartile: OR = 0.516, 95% CI 0.361–0.739). Intake of fatty fish contributed most strongly to this protective effect (EPA highest vs. lowest quartile: OR = 0.556, 95% CI 0.390–0.794; DHA highest vs. lowest quartile: OR = 0.575, 95% CI 0.403–0.820), whereas mollusks, crustaceans, and some processed/frozen fish showed weaker or no associations. A statistically significant trend of decreasing MASLD risk was observed across increasing quartiles of both EPA and DHA intake. Conclusions: Higher intake of EPA and DHA, especially from fatty fish, is linked to lower MASLD risk in this Mediterranean population, supporting recommendations for regular fish consumption to protect liver health. Full article

(This article belongs to the Special Issue Anti-Inflammatory and Anti-Oxidative Bioactive Compounds in Diet and Their Applications)

► Show Figures

Figure 1

25 pages, 778 KB

Open AccessArticle

A Brief Participatory Workplace Intervention on Dietary Barriers and Healthy Eating Intentions Among Employees: A Pilot Study

by Aleksandra Hyży, Ilona Cieślak, Joanna Gotlib-Małkowska, Mariusz Panczyk and Mariusz Jaworski

Nutrients 2025, 17(21), 3371; https://doi.org/10.3390/nu17213371 - 27 Oct 2025

Abstract

Background: An unhealthy diet is a major modifiable risk factor for chronic disease, and workplace environments may pose specific barriers to healthy eating. Methods: This single-arm, pre–post workplace intervention assessed short-term changes in perceived dietary barriers (primary outcome) and healthy eating intentions (secondary [...] Read more.

Background: An unhealthy diet is a major modifiable risk factor for chronic disease, and workplace environments may pose specific barriers to healthy eating. Methods: This single-arm, pre–post workplace intervention assessed short-term changes in perceived dietary barriers (primary outcome) and healthy eating intentions (secondary outcome) among school employees (n = 36; 83.3% women; mean age = 46.8 years). The 60 min participatory session integrated behavioral economics principles, practical nutrition exercises, and psychological strategies designed to enhance self-efficacy, optimism, and resilience. Results: The total perceived barrier score decreased significantly (Δ = −1 [IQR −2 to 0]; the paired Wilcoxon signed-rank test = −4.689, p < 0.001, r = −0.78). Post-intervention (T2), the proportion of participants reporting an intention to prepare healthy meals increased (McNemar’s test, p < 0.001; OR = 10.0, 95% CI 1.8–54.5). The session was rated as highly useful (mean = 8.3/10), and at the three-week follow-up (T3), 91.7% of participants reported maintaining at least one dietary change. Conclusions: Although limited by its single-arm design and short follow-up, this pilot study suggests that a brief, participatory behavioral intervention may be a feasible and acceptable approach to support healthier eating in the workplace. Full article

(This article belongs to the Special Issue Workplace Health Strategy: Dietary and Nutrition Influence)

► Show Figures

Figure 1

Journal Description

Nutrients

Latest Articles

Journal Menu

Journal Browser

Highly Accessed Articles

Latest Books

E-Mail Alert

News

Topics

Conferences

Special Issues

Topical Collections

Conference Reports

Further Information

Guidelines

MDPI Initiatives

Follow MDPI