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Microorganisms, Volume 7, Issue 7

2019 July - 29 articles

Cover Story: Shiga toxin-producing Escherichia coli (STEC) strains are associated with human diarrhea, and some strains may cause hemolytic–uremic syndrome. The pathogenesis of STEC in intestinal illness usually entails bacterial attachment to the intestinal epithelial cells, microvillus destruction, and Shiga toxin translocation. In this article, a system biology approach was used to investigate the transcriptomic and phenotypic responses of enterocyte-like Caco-2 cells to STEC strains. Serial scanning electron microscopy (SEM) images and temporal dynamic gene co-expression networks (GCN) for Caco-2 cells were obtained during three hours of enterocyte-STEC interaction. SEM showed a bacterial adherence to the cells and a progressive loss of microvilli. GCN analysis revealed network topological variation during enterocyte–STEC interaction. View this paper.
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Articles (29)

  • Article
  • Open Access
43 Citations
6,653 Views
15 Pages

Background: Microorganisms are widespread in all environments, including in and on animal bodies. The gut microbiome has an essential influence on fish health, and is affected by several persistent and harmful organic and inorganic contaminants. Cons...

  • Article
  • Open Access
17 Citations
5,766 Views
14 Pages

Phylogenetic and Molecular Profile of Staphylococcus aureus Isolated from Bloodstream Infections in Northeast Brazil

  • Andrea de S. Monteiro,
  • Bruna L. S. Pinto,
  • Joveliane de M. Monteiro,
  • Rômulo M. Ferreira,
  • Patrícia C. S. Ribeiro,
  • Silvia Y. Bando,
  • Sirlei G. Marques,
  • Luís C. N. Silva,
  • Wallace R. Nunes Neto and
  • Afonso G. Abreu
  • + 2 authors

Staphylococcus aureus is a notorious human pathogen associated with serious nosocomial and community-acquired infections, such as pneumonia, meningitis, endocarditis, toxic shock syndrome, and sepsis, among others. The objective of this study was to...

  • Article
  • Open Access
4 Citations
4,674 Views
12 Pages

Glaciers have been recognized as biomes, dominated by microbial life. Many novel species have been isolated from glacier ecosystems, and their physiological features are well characterized. However, genomic features of bacteria isolated from the deep...

  • Article
  • Open Access
72 Citations
6,685 Views
20 Pages

Safe Cultivation of Medicago sativa in Metal-Polluted Soils from Semi-Arid Regions Assisted by Heat- and Metallo-Resistant PGPR

  • Anas Raklami,
  • Khalid Oufdou,
  • Abdel-Ilah Tahiri,
  • Enrique Mateos-Naranjo,
  • Salvadora Navarro-Torre,
  • Ignacio D. Rodríguez-Llorente,
  • Abdelilah Meddich,
  • Susana Redondo-Gómez and
  • Eloísa Pajuelo

Soil contamination with heavy metals is a constraint for plant establishment and development for which phytoremediation may be a solution, since rhizobacteria may alleviate plant stress under these conditions. A greenhouse experiment was conducted to...

  • Article
  • Open Access
53 Citations
5,587 Views
13 Pages

Upgrading Grape Pomace through Pleurotus spp. Cultivation for the Production of Enzymes and Fruiting Bodies

  • Aikaterini Papadaki,
  • Vasiliki Kachrimanidou,
  • Seraphim Papanikolaou,
  • Antonios Philippoussis and
  • Panagiota Diamantopoulou

Grape pomace, a by-product derived from winery industries, was used as fermentation media for the production of added-value products through the cultivation of two Pleurotus species. Solid-state (SSF), semiliquid (SLF), and submerged (SmF) fermentati...

  • Review
  • Open Access
112 Citations
9,386 Views
14 Pages

Zearalenone (ZEA) is a mycotoxin produced by Fusarium fungi that is commonly found in cereal crops. ZEA has an estrogen-like effect which affects the reproductive function of animals. It also damages the liver and kidneys and reduces immune function...

  • Article
  • Open Access
16 Citations
5,826 Views
15 Pages

Engineering Synthetic Microbial Communities through a Selective Biofilm Cultivation Device for the Production of Fermented Beverages

  • Sokny Ly,
  • F. Bajoul Kakahi,
  • Hasika Mith,
  • Chanvorleak Phat,
  • Barbara Fifani,
  • Tierry Kenne,
  • Marie-Laure Fauconnier and
  • Frank Delvigne

Production of Cambodian rice wine involves complex microbial consortia. Indeed, previous studies focused on traditional microbial starters used for this product revealed that three microbial strains with complementary metabolic activities are require...

  • Article
  • Open Access
15 Citations
6,797 Views
20 Pages

Prokaryotes do not make use of a nucleus membrane to segregate their genetic material from the cytoplasm, so that their nucleoid is potentially free to explore the whole volume of the cell. Nonetheless, high resolution images of bacteria with very co...

  • Article
  • Open Access
2 Citations
4,528 Views
9 Pages

Arthonia ulleungdoensis Lee & Hur is described as a new lichen species from South Korea. The new species is distinguishable from Arthonia ruana A. Massal. by its large, rounded and non-punctiform apothecia, taller apothecial section, asci with fe...

  • Article
  • Open Access
27 Citations
5,239 Views
16 Pages

Effects of Chestnut Tannin Extract, Vescalagin and Gallic Acid on the Dimethyl Acetals Profile and Microbial Community Composition in Rumen Liquor: An In Vitro Study

  • Federica Mannelli,
  • Matteo Daghio,
  • Susana P. Alves,
  • Rui J. B. Bessa,
  • Sara Minieri,
  • Luciana Giovannetti,
  • Giuseppe Conte,
  • Marcello Mele,
  • Anna Messini and
  • Arianna Buccioni
  • + 2 authors

The addition of polyphenol extracts in ruminant diets is an effective strategy to modulate rumen microflora. The aim of this in vitro trial was to study the effects of chestnut tannin extract (CHT), vescalagin (VES) and gallic acid (GAL) on dietary f...

  • Article
  • Open Access
18 Citations
6,161 Views
22 Pages

Phylotypic Characterization of Mycobionts and Photobionts of Rock Tripe Lichen in East Antarctica

  • Merry Sailonga Faluaburu,
  • Ryosuke Nakai,
  • Satoshi Imura and
  • Takeshi Naganuma

Saxicolous rock ripe lichens that grow on rocks in the East Antarctic fellfields were sampled for phylotypic characterization of its constituent mycobionts (fungi) and photobionts (algae and cyanobacteria). The rock tripe lichen-forming fungal and al...

  • Article
  • Open Access
3 Citations
3,787 Views
13 Pages

Phenotypic and genotypic evidence indicates that many LAB strains can grow in presence of oxygen and can shift from fermentative to aerobic and/or respiratory metabolism. The aerobic and respiratory growth of several LAB species have been studied, al...

  • Article
  • Open Access
12 Citations
3,879 Views
9 Pages

Candida species represent one of the most frequent causes of hospital-acquired infections in immunocompromised patient cohorts. Due to a very limited set of antifungals available and an increasing prevalence of drug resistance, the discovery of novel...

  • Review
  • Open Access
59 Citations
12,094 Views
22 Pages

The Host-Microbe Interplay in Human Papillomavirus-Induced Carcinogenesis

  • Rei Wakabayashi,
  • Yusuke Nakahama,
  • Viet Nguyen and
  • J. Luis Espinoza

Every year nearly half a million new cases of cervix cancer are diagnosed worldwide, making this malignancy the fourth commonest cancer in women. In 2018, more than 270,000 women died of cervix cancer globally with 85% of them being from developing c...

  • Article
  • Open Access
22 Citations
8,459 Views
19 Pages

Functional and Pharmacological Analyses of the Role of Penicillium digitatum Proteases on Virulence

  • Ana-Rosa Ballester,
  • Mario López-Pérez,
  • Beatriz de la Fuente and
  • Luis González-Candelas

Penicillium digitatum is the major postharvest pathogen of citrus fruit under Mediterranean climate conditions. Previous results have shown that proteases is the largest enzyme family induced by P. digitatum during fruit infection. In the present wor...

  • Article
  • Open Access
9 Citations
4,083 Views
15 Pages

Winning the War against Multi-Drug Resistant Diarrhoeagenic Bacteria

  • Chizoba Mercy Enemchukwu,
  • Angus Nnamdi Oli,
  • Ebere Innocent Okoye,
  • Nonye Treasure Ujam,
  • Emmanuel O. Osazuwa,
  • George Ogonna Emechebe,
  • Kenneth Nchekwube Okeke,
  • Christian Chukwuemeka Ifezulike,
  • Obiora Shedrack Ejiofor and
  • Jude Nnaemeka Okoyeh

Drug-resistant-diarrhoeagenic bacteria are currently emerging healthcare challenge. This study investigated the effects of Vernonia amygdalina, Garcinia kola, tetracycline and metronidazole combinations on such bacteria. Agar well diffusion method wa...

  • Communication
  • Open Access
3 Citations
7,057 Views
11 Pages

Salmonella enterica is one of the most prevalent foodborne pathogens. The large quantity of serovar types results in the colonization of a large spectrum of hosts, with different environmental conditions and hazards. The aim of this study was to eval...

  • Article
  • Open Access
6 Citations
5,663 Views
23 Pages

Dynamic Gene Network Analysis of Caco-2 Cell Response to Shiga Toxin-Producing Escherichia coli-Associated Hemolytic–Uremic Syndrome

  • Silvia Y. Bando,
  • Priscila Iamashita,
  • Filipi N. Silva,
  • Luciano da F. Costa,
  • Cecilia M. Abe,
  • Fernanda B. Bertonha,
  • Beatriz E. C. Guth,
  • André Fujita and
  • Carlos A. Moreira-Filho

Shiga toxin-producing Escherichia coli (STEC) O113:H21 strains are associated with human diarrhea and some strains may cause hemolytic–uremic syndrome (HUS). In Brazil, these strains are commonly found in cattle but, so far, were not isolated f...

  • Article
  • Open Access
57 Citations
6,223 Views
16 Pages

Sichuan pickle is a traditional fermented food in China which is produced by the spontaneous fermentation of Chinese cabbage. In this study, the anti-obesity effects of a new lactic acid bacterium (Lactobacillus fermentum CQPC05, LF-CQPC05) isolated...

  • Article
  • Open Access
9 Citations
4,707 Views
17 Pages

Aeromonas salmonicida subsp. salmonicida is a Gram-negative bacterium causing furunculosis, an opportunistic infection of farmed salmonid fish. Current treatment methods against furunculosis rely heavily on antibiotherapy. However, strains of this op...

  • Article
  • Open Access
9 Citations
9,530 Views
40 Pages

DNA Damage Response Pathways in Dinoflagellates

  • Chongping Li and
  • Joseph Tin Yum Wong

Dinoflagellates are a general group of phytoplankton, ubiquitous in aquatic environments. Most dinoflagellates are non-obligate autotrophs, subjected to potential physical and chemical DNA-damaging agents, including UV irradiation, in the euphotic zo...

  • Article
  • Open Access
9 Citations
5,277 Views
12 Pages

Overexpression of RAD51 Enables PCR-Based Gene Targeting in Lager Yeast

  • Beatrice Bernardi,
  • Yeseren Kayacan,
  • Madina Akan and
  • Jürgen Wendland

Lager beer fermentations rely on specific polyploid hybrids between Saccharomyces cerevisiae and Saccharomyces eubayanus falling into the two groups of S. carlsbergensis/Saaz-type and S. pastorianus/Frohberg-type. These strains provide a terroir to l...

  • Review
  • Open Access
14 Citations
6,045 Views
18 Pages

Glycan Utilisation and Function in the Microbiome of Weaning Infants

  • Starin McKeen,
  • Wayne Young,
  • Karl Fraser,
  • Nicole C. Roy and
  • Warren C. McNabb

Glycans are present exogenously in the diet, expressed and secreted endogenously by host cells, and produced by microbes. All of these processes result in them being available to the gut microbiome, firmly placing glycans at the interface of diet&nda...

  • Article
  • Open Access
32 Citations
6,995 Views
14 Pages

The consumption of dietary supplements to treat health complications or to improve overall health conditions has become a globally increasing trend that leads to the development of a large number of health-related novel products and expands the assoc...

  • Review
  • Open Access
49 Citations
7,569 Views
15 Pages

Effect of Different Laser Wavelengths on Periodontopathogens in Peri-Implantitis: A Review of In Vivo Studies

  • Katarzyna Świder,
  • Marzena Dominiak,
  • Kinga Grzech-Leśniak and
  • Jacek Matys

Nowadays, many studies are examining the effectiveness of dental lasers in the treatment of peri-implantitis; however, most of them only report periodontal parameter changes. The authors of this review tried to address the question: “What is th...

  • Review
  • Open Access
193 Citations
24,834 Views
46 Pages

Multifaceted Applications of Microbial Pigments: Current Knowledge, Challenges and Future Directions for Public Health Implications

  • Chatragadda Ramesh,
  • Nambali Valsalan Vinithkumar,
  • Ramalingam Kirubagaran,
  • Chidambaram Kulandaisamy Venil and
  • Laurent Dufossé

Microbial oddities such as versatile pigments are gaining more attention in current research due to their widely perceived applications as natural food colorants, textiles, antimicrobial activities, and cytotoxic activities. This indicates that the f...

  • Case Report
  • Open Access
5 Citations
3,743 Views
11 Pages

An Asymptomatic Patient with Fatal Infertility Carried a Swedish Strain of Chlamydia trachomatis with Additional Deletion in The Plasmid orf1 that Belonged to A Different MLST Sequence Type

  • Valentina A. Feodorova,
  • Sergey S. Zaitsev,
  • Yury V. Saltykov,
  • Edgar S. Sultanakhmedov,
  • Andrew L. Bakulev,
  • Sergey S. Ulyanov and
  • Vladimir L. Motin

Here, we present the first case of asymptomatic genital Chlamydial infection caused by the new emerging Chlamydia trachomatis (C.t.) ST13 strain genovar E, which has a double deletion of 377 bp and 17 bp in orf1 gene of the cryptic plasmid (ddCT). Th...

  • Opinion
  • Open Access
3 Citations
3,766 Views
5 Pages

Despite the obvious impact of tuberculosis on global health, there is currently no effective vaccine and there is increasing resistance against established front-line drug regiments. Our current understanding of disease progression in tuberculosis is...

  • Review
  • Open Access
72 Citations
7,192 Views
13 Pages

West Nile virus (WNV) and Usutu virus (USUV) are neurotropic mosquito-borne flaviviruses that may infect humans. Although WNV is much more widespread and plays a much larger role in human health, the two viruses are characterized by similar envelope...

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Microorganisms - ISSN 2076-2607