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Microorganisms, Volume 13, Issue 10

October 2025 - 193 articles

Cover Story: Donor human milk is an essential nutritional source for preterm infants, yet its microbiological safety remains a critical concern. In this study, four culture-based methods were systematically compared to evaluate their sensitivity for detecting Enterobacterales in DHM. By combining standardized spiking experiments with clinically relevant isolates and analysis of raw donor milk linked to a suspected transmission event, method-specific detection limits were determined. The results revealed marked differences in sensitivity, particularly at low bacterial loads, where contamination may go undetected. These findings underscore the need for harmonized testing protocols and evidence-based thresholds to ensure neonatal safety and inform future regulatory guidance. View this paper
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Articles (193)

  • Article
  • Open Access
533 Views
18 Pages

Unlocking the Potential of vB_EfaS_LOK1: A Newly Isolated Bacteriophage Against Enterococcus faecalis

  • Laura Marcela Plata Suarez,
  • Salvador Del Valle Balbuena,
  • Isamar Leticia Becerra Mejía,
  • Alejandra Aidee Loera Piedra,
  • Cristina Domínguez Espinoza,
  • Arantxa Monserrat Ángeles González,
  • Araceli Contreras Rodríguez,
  • Alejandra Aquino Andrade,
  • Sergio Francisco Martínez Díaz and
  • Ma. Guadalupe Aguilera Arreola

Phage therapy has emerged as a promising alternative for combating infections caused by drug-resistant pathogens. Among these, Enterococcus faecalis remains a significant public health concern due to its persistence in clinical settings and frequent...

  • Review
  • Open Access
460 Views
18 Pages

Bacterial extracellular vesicles (BEVs) are nanoscale membrane-bound structures secreted by prokaryotic cells and have recently gained considerable attention in environmental pollution research. By encapsulating virulence factors and antibiotic resis...

  • Article
  • Open Access
341 Views
11 Pages

Essential Oils as a Novel Anti-Biofilm Strategy Against Salmonella Enteritidis Isolated from Chicken Meat

  • Suzana Vidaković Knežević,
  • Slobodan Knežević,
  • Dubravka Milanov,
  • Jelena Vranešević,
  • Marko Pajić,
  • Sunčica Kocić-Tanackov and
  • Nedjeljko Karabasil

Salmonella Enteritidis is a serious foodborne threat, being the most reported Salmonella serovar in the past several years. Biofilm formation contributes significantly to its persistence and resistance in food processing environments, making it harde...

  • Review
  • Open Access
446 Views
27 Pages

Bacterial vaginosis is more than a mild inconvenience for women and has been shown to be an important cause of morbidity and mortality in women through sexually transmitted infections, and in babies due to late miscarriage and preterm birth. The aeti...

  • Systematic Review
  • Open Access
750 Views
39 Pages

Natural Molecules, Nutraceuticals, and Engineered Nanosystems: A Comprehensive Strategy for Combating Gardnerella vaginalis-Induced Bacterial Vaginosis

  • Manoj Dalabehera,
  • Abdulrahman Mohammed Alhudhaibi,
  • Emad M. Abdallah,
  • Tarek H. Taha,
  • Shubham Chaudhari,
  • Alka Kumari,
  • Rudra Narayan Subudhi and
  • Charul Rathore

Bacterial vaginosis (BV) is a highly prevalent vaginal infection characterized by a dysbiotic shift in the vaginal microbiota, with Gardnerella vaginalis acting as a principal pathogen. Despite its association with adverse reproductive outcomes, BV r...

  • Article
  • Open Access
572 Views
12 Pages

Tegoprazan (TPZ) has the potential to enhance Helicobacter pylori eradication. This study aimed to investigate the efficacy of TPZ–amoxicillin (TA) dual therapy against clarithromycin-resistant H. pylori strains. All H. pylori-positive patients...

  • Article
  • Open Access
259 Views
15 Pages

SpuA-Mediated Glycogen Metabolism Modulates Acid Stress Adaptation via Formic Acid and Amino Acid Utilization in Streptococcus pneumoniae

  • Weichen Gong,
  • Masayuki Ono,
  • Xuefei Cheng,
  • Yujiro Hirose,
  • Keita Nishiyama,
  • Haruki Kitazawa and
  • Shigetada Kawabata

Glycogen metabolism plays a key role in bacterial adaptation. In Streptococcus pneumoniae, the glycogen-degrading enzyme SpuA is widely conserved, but its physiological significance remains unclear. In this study, we investigated how SpuA affects bac...

  • Article
  • Open Access
369 Views
17 Pages

Vulnerability of Walnut Pruning Wounds to Fungal Trunk Pathogens and Seasonal Conidial Dynamics of Botryosphaeriaceae in the Maule Region, Chile

  • Shehzad Iqbal,
  • Iqra Mubeen,
  • Mauricio Lolas,
  • Ernesto Moya-Elizondo,
  • Pedro Gundel,
  • Samuel Ortega-Farias,
  • William Campillay-Llanos and
  • Gonzalo A. Díaz

Branch canker and dieback, caused by Botryosphaeriaceae and Diaporthaceae, is a major disease of walnut (Juglans regia L.) worldwide. In Chile, the impact of pruning wound age and timing on susceptibility to these pathogens in walnut trees remains po...

  • Article
  • Open Access
287 Views
14 Pages

Urobiome Signatures of Recurrent Urinary Tract Infections in Adolescent Pregnancy: A Longitudinal Study

  • Carlos Daniel Mora-Vargas,
  • Orly Grobeisen-Duque,
  • Oscar Villavicencio-Carrisoza,
  • Diana Angélica Aguilar-Ayala,
  • Orlando Castellanos-Diaz,
  • Maria Guadalupe Martinez-Salazar,
  • Alejandro Rosas-Balan,
  • Veronica Flores-Rueda,
  • Moises Leon-Juarez and
  • Mario Guzman-Huerta
  • + 5 authors

Adolescent pregnancy is a significant public health concern, with maternal and fetal risks compounded by pregnancy-related anatomical, hormonal, and urinary changes that predispose to urinary tract infections (UTIs). Alterations in the urinary microb...

  • Article
  • Open Access
902 Views
13 Pages

The amphibian microbiome plays a vital role in host health, yet the bacterial communities of caecilians (Order: Gymnophiona) remain largely uncharacterised. We investigated this by providing the first characterisation of the gut and skin microbiome o...

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Microorganisms - ISSN 2076-2607Creative Common CC BY license