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Open AccessArticle

Engineering Synthetic Microbial Communities through a Selective Biofilm Cultivation Device for the Production of Fermented Beverages

1
Terra Research and Teaching Centre, Microbial Processes and Interactions, Gembloux Agro-Bio Tech, University of Liège, Gembloux Agro-Bio Tech, 5030 Gembloux, Belgium
2
Faculty of Chemical and Food Engineering, Institute of Technology of Cambodia, Phnom Penh 12156, Cambodia
3
General and Organic Chemistry, Gembloux Agro-BioTech, University of Liège, 5030 Gembloux, Belgium
*
Author to whom correspondence should be addressed.
Microorganisms 2019, 7(7), 206; https://doi.org/10.3390/microorganisms7070206
Received: 25 June 2019 / Revised: 16 July 2019 / Accepted: 18 July 2019 / Published: 20 July 2019
(This article belongs to the Special Issue Microbial Dynamics in Wine Production)
Production of Cambodian rice wine involves complex microbial consortia. Indeed, previous studies focused on traditional microbial starters used for this product revealed that three microbial strains with complementary metabolic activities are required for an effective fermentation, i.e., filamentous fungi (Rhizopus oryzae), yeast (Saccharomyces cerevisiae), and lactic acid bacteria (Lactobacillus plantarum). Modulating the ratio between these three key players led to significant differences, not only in terms of ethanol and organic acid production, but also on the profile of volatile compounds, in comparison with natural communities. However, we observed that using an equal ratio of spores/cells of the three microbial strains during inoculation led to flavor profile and ethanol yield close to that obtained through the use of natural communities. Compartmentalization of metabolic tasks through the use of a biofilm cultivation device allows further improvement of the whole fermentation process, notably by increasing the amount of key components of the aroma profile of the fermented beverage (i.e., mainly phenylethyl alcohol, isobutyl alcohol, isoamyl alcohol, and 2-methyl-butanol) and reducing the amount of off-flavor compounds. This study is a step forward in our understanding of interkingdom microbial interactions with strong application potential in food biotechnology. View Full-Text
Keywords: microbial interactions; volatolomic; biofilm; alcoholic fermentation microbial interactions; volatolomic; biofilm; alcoholic fermentation
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Ly, S.; Bajoul Kakahi, F.; Mith, H.; Phat, C.; Fifani, B.; Kenne, T.; Fauconnier, M.-L.; Delvigne, F. Engineering Synthetic Microbial Communities through a Selective Biofilm Cultivation Device for the Production of Fermented Beverages. Microorganisms 2019, 7, 206.

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