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Microbiology Research, Volume 15, Issue 1

2024 March - 28 articles

Cover Story: Clostridioides difficile is an anaerobic bacterium recognized as the main cause of diarrhea associated with the use of antibiotics in hospitals. The epidemiology of C. difficile infection (CDI) has recently evolved, with an increased incidence of CDI in the community and increased detection of C. difficile from animals, food, and the environment. C. difficile’s genomic evolution is associated with the international emergence and spread of new highly virulent ribotypes (RTs) that have an important impact on global public health and the need for constant monitoring to effectively prevent and control CDI. In this study, the variable cdtR gene was used as a target to develop a molecular beacon-based real-time PCR method to rapidly and easily type C. difficile strains belonging to different RTs and evolutionary clades of One Health importance. View this paper
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Articles (28)

  • Brief Report
  • Open Access
6 Citations
5,666 Views
9 Pages

Background: The detection of neutralizing anti-SARS-CoV-2 antibodies is important since they represent the subset of antibodies able to prevent the virus to invade human cells. The aim of this study is to evaluate the clinical performances of an in-h...

  • Article
  • Open Access
13 Citations
6,007 Views
18 Pages

Effects of Biochar and Arbuscular Mycorrhizal Fungi on Soil Health in Chinese Kale (Brassica oleracea var. alboglabra L.) Cultivation

  • Kritsana Jatuwong,
  • Worawoot Aiduang,
  • Tanongkiat Kiatsiriroat,
  • Wassana Kamopas and
  • Saisamorn Lumyong

Biochar and arbuscular mycorrhizal fungi (AMF), a promising environmentally friendly soil enhancer and biostimulant, play a crucial role in sustainable agriculture by influencing soil properties and plant growth. This research investigates the chemic...

  • Article
  • Open Access
2 Citations
4,450 Views
19 Pages

Virulence and Antimicrobial Resistance Profiles of Shiga Toxin-Producing Escherichia coli from River Water and Farm Animal Feces near an Agricultural Region in Northwestern Mexico

  • Bianca A. Amézquita-López,
  • Marcela Soto-Beltrán,
  • Bertram G. Lee,
  • Edgar F. Bon-Haro,
  • Ofelia Y. Lugo-Melchor and
  • Beatriz Quiñones

Shiga toxin-producing Escherichia coli (STEC) are zoonotic enteric pathogens linked to human gastroenteritis worldwide. To aid the development of pathogen control efforts, the present study characterized the genotypic diversity and pathogenic potenti...

  • Article
  • Open Access
1,666 Views
14 Pages

Antagonistic Activity of Macrolepiota sp. CS185 against Post-Harvest Fungi of Fig Fruits (Ficus carica L.)

  • Guadalupe Gutiérrez-Soto,
  • Iosvany López-Sandin,
  • Jesús Salvador Hernández Ochoa,
  • Carlos Eduardo Hernadez-Luna,
  • Juan Francisco Contreras-Cordero and
  • Carlos Alberto Hernández-Martínez

Macrolepiota sp. CS185 is a basidiomycete with high potential as a biocontrol agent against various phytopathogenic fungi. Regardless of its pronounced potential as a post-harvest fungi biocontrol agent, its activity in tomato seedlings infected with...

  • Article
  • Open Access
2,362 Views
17 Pages

C. difficile infection (CDI) has an important impact on both human and animal health. The rapid detection and monitoring of C. difficile PCR-ribotypes (RTs) cause of CDI is critical to control and prevent this infection. This study reports the first...

  • Article
  • Open Access
5 Citations
3,076 Views
12 Pages

Influence of Organic Matter from Native Fish on the Antimicrobial Efficacy of Sodium Hypochlorite (NaClO) in Reducing Salmonella spp. Population

  • Jaqueline Oliveira Reis,
  • Carine Baggio Cavalcante,
  • Nathaly Barros Nunes,
  • Adelino Cunha Neto,
  • Maxsueli Aparecida Moura Machado,
  • Yuri Duarte Porto,
  • Vinicius Silva Castro and
  • Eduardo Eustáquio de Souza Figueiredo

29 February 2024

Salmonella spp. is not part of the fish microbiota, being introduced through contaminated water or improper handling. In slaughterhouses, five parts per million (ppm) of free chlorine is recommended in the washing step to eliminate this pathogen. How...

  • Article
  • Open Access
5 Citations
3,507 Views
16 Pages

26 February 2024

Selenium nanoparticles (SeNPs) have greater bioavailability and safety than inorganic selenium, and was widely used in medical, agricultural, nutritional supplements, and antibacterial fields. The present study screened a strain L11 producing SeNPs f...

  • Article
  • Open Access
2,094 Views
12 Pages

The Impact of pH Modulation on the Rhizosphere Bacteriome of Maize and Bean

  • Rodrigo Reis Moura,
  • Douglas Alfradique Monteiro,
  • Eduardo da Silva Fonseca,
  • Fabiano de Carvalho Balieiro,
  • Fernando Cesário and
  • Caio Tavora Coelho da Costa Rachid

20 February 2024

In soils, pH stands as the main factor modulating bacterial communities’ composition. However, most studies address its effects in bulk soils in natural systems, with few focusing on its effects in the rhizosphere of plants. Predicting pH effec...

  • Article
  • Open Access
2 Citations
2,754 Views
24 Pages

17 February 2024

Antimicrobial resistance poses a severe threat, particularly in developing countries where the ready availability of drugs and increased consumption lead to improper antibiotic usage, thereby causing a surge in resistance levels compared to developed...

  • Article
  • Open Access
7 Citations
2,795 Views
17 Pages

In Silico Identification of Sugarcane Genome-Encoded MicroRNAs Targeting Sugarcane Mosaic Virus

  • Wang Wenzhi,
  • Muhammad Aleem Ashraf,
  • Hira Ghaffar,
  • Zainab Ijaz,
  • Waqar ul Zaman,
  • Huda Mazhar,
  • Maryam Zulfqar and
  • Shuzhen Zhang

16 February 2024

Sugarcane mosaic virus (SCMV) (genus, Potyvirus; family, Potyviridae) is widespread, deleterious, and the most damaging pathogen of sugarcane (Saccharum officinarum L. and Saccharum spp.) that causes a substantial barrier to producing high sugarcane...

  • Review
  • Open Access
32 Citations
14,837 Views
26 Pages

Microbial Cell Factories: Biodiversity, Pathway Construction, Robustness, and Industrial Applicability

  • Rida Chaudhary,
  • Ali Nawaz,
  • Mireille Fouillaud,
  • Laurent Dufossé,
  • Ikram ul Haq and
  • Hamid Mukhtar

12 February 2024

The microbial biosynthesis of proteins, primary metabolites, and chemicals is gaining extraordinary momentum and is presently viewed as an advancing approach in the industrial research sector. Increased threats to the environment and the possibility...

  • Article
  • Open Access
1 Citations
2,057 Views
11 Pages

Assessment of the Possibility of Using Bacterial Strains and Bacteriophages for Epidemiological Studies in the Bioaerosol Environment

  • Radosław Wróbel,
  • Monika Andrych-Zalewska,
  • Jędrzej Matla,
  • Justyna Molska,
  • Gustaw Sierzputowski,
  • Agnieszka Szulak,
  • Radosław Włostowski,
  • Adriana Włóka and
  • Małgorzata Rutkowska-Gorczyca

During the COVID-19 pandemic, microbiological controls neglected the spread of viruses through the air. Techniques to identify this threat required additional research to enable control measures to be introduced to protect against the spread of disea...

  • Article
  • Open Access
12 Citations
3,506 Views
11 Pages

A Role for Secondary Metabolites in Desiccation Tolerance in Lichens

  • Nqobile Truelove Ndhlovu,
  • Farida Minibayeva and
  • Richard Peter Beckett

In lichens, secondary metabolites have been shown to protect against biotic stresses such as pathogen attacks and grazing, and abiotic stresses such as ultraviolet (UV) and high photosynthetically active radiation (PAR). Lichen secondary metabolites...

  • Article
  • Open Access
8 Citations
2,510 Views
12 Pages

COVID-19 Epidemic Process and Evolution of SARS-CoV-2 Genetic Variants in the Russian Federation

  • Vasiliy Akimkin,
  • Tatiana A. Semenenko,
  • Svetlana V. Ugleva,
  • Dmitry V. Dubodelov and
  • Kamil Khafizov

The COVID-19 pandemic, etiologically related to a new coronavirus, has had a catastrophic impact on the demographic situation on a global scale. The aim of this study was to analyze the manifestations of the COVID-19 epidemic process, the dynamics of...

  • Article
  • Open Access
8 Citations
3,075 Views
17 Pages

Promising Eco-Friendly Nanoparticles for Managing Bottom Rot Disease in Lettuce (Lactuca sativa var. longifolia)

  • Nashwa A. H. Fetyan,
  • Tarek A. Essa,
  • Tamer M. Salem,
  • Ahmed Aboueloyoun Taha,
  • Samah Fawzy Elgobashy,
  • Nagwa A. Tharwat and
  • Tamer Elsakhawy

Developing innovative, eco-friendly fungicide alternatives is crucial to mitigate the substantial threat fungal pathogens pose to crop yields. In this study, we assessed the in vitro effectiveness of SiO2, CuO, and γFe2O3 nanoparticles against...

  • Communication
  • Open Access
6 Citations
2,300 Views
9 Pages

Effects of Glomus fasciculatum, Azotobacter chroococcum and Vermicompost Leachate on the Production and Quality of Tomato Fruit

  • Alejandro Alarcón-Zayas,
  • Luis Guillermo Hernández-Montiel,
  • Diana Medina-Hernández,
  • Edgar Omar Rueda-Puente,
  • Wilson Geobel Ceiro-Catasú and
  • Ramón Jaime Holguín-Peña

Arbuscular mycorrhizal fungi, plant-growth-promoting bacteria (PGPB) and vermicompost constitute important environmental and economic resources for improving the production and quality of tomato fruits. The present research aims to determine the sing...

  • Article
  • Open Access
3 Citations
2,296 Views
13 Pages

The current investigation delved into the utilization of cattle and municipal sanitary inocula for anaerobic digestion of poultry wastes, addressing a crucial and pragmatic challenge in waste management. The emphasis on poultry waste is pertinent due...

  • Case Report
  • Open Access
11 Citations
6,568 Views
10 Pages

Coffee leaf rust (CLR) is a major disease of Arabica coffee caused by the biotrophic fungus Hemileia vastatrix. Jazan region in Saudi Arabia has long been one of the last coffee-producing regions in the world free of this disease. In August 2023, CLR...

  • Article
  • Open Access
9 Citations
3,524 Views
12 Pages

Screening of Microorganisms from Wastes and Identification of the Optimal Substrate for Biosurfactant Production

  • Liliya Biktasheva,
  • Alexander Gordeev,
  • Anastasia Kirichenko,
  • Polina Kuryntseva and
  • Svetlana Selivanovskaya

The production of biosurfactants from organic wastes has received significant attention due to its potential cost savings. This study involved the isolation of biosurfactant-producing microorganisms from waste sources. The surfactant properties of th...

  • Review
  • Open Access
8 Citations
6,983 Views
15 Pages

Updates on Staphylococcal Vaccines

  • Alexandru Scafa-Udriste,
  • Mircea-Ioan Popa and
  • Gabriela-Loredana Popa

28 December 2023

Staphylococcus aureus, a prevalent human pathogen and a leading cause of hospital-acquired infections, is increasingly evolving antibiotic-resistant strains, increasing mortality and morbidity rates. Anti-staphylococcal vaccine research for preventio...

  • Article
  • Open Access
2,456 Views
17 Pages

Automated Protocol for Monitoring Droplets and Fomites on Surfaces

  • Federica Valeriani,
  • Lory Marika Margarucci,
  • Francesca Ubaldi,
  • Gianluca Gianfranceschi and
  • Vincenzo Romano Spica

27 December 2023

During the COVID-19 pandemic, extensive efforts focused on developing a better understanding of indirect transmission routes, environmental monitoring of fomites, and suitable surveillance strategies, providing new perspectives to also face other com...

  • Communication
  • Open Access
1 Citations
3,687 Views
11 Pages

26 December 2023

Healthy individuals often have different gut microbiota, and these differences can be influenced by their country of origin or their race. This study aimed to compare the gut microbiota compositions of healthy Germans and Koreans using 16S rRNA seque...

  • Article
  • Open Access
7 Citations
8,931 Views
18 Pages

Assess the Diagnostic Accuracy of GeneXpert to Detect Mycobacterium tuberculosis and Rifampicin-Resistant Tuberculosis among Presumptive Tuberculosis and Presumptive Drug Resistant Tuberculosis Patients

  • Venkateswari Ramachandra,
  • Usharani Brammacharry,
  • Aaina Muralidhar,
  • Anbazhagi Muthukumar,
  • Revathi Mani,
  • Muthuraj Muthaiah,
  • Govindarajan Soundappan and
  • Asha Frederick

GeneXpert MTB/RIF is a rapid molecular diagnostic tool capable of simultaneously detecting Mycobacterium tuberculosis and rifampicin resistance. This study aimed to assess the diagnostic precision of GeneXpert MTB/RIF assay to detect pulmonary and ex...

  • Review
  • Open Access
3 Citations
2,794 Views
25 Pages

This article explores the diverse array of biologically active compounds derived from microbial symbionts, particularly focusing on the isolation and characterization of diepoxides, highly oxygenated triterpenoids, secosteroids, ergostane-type steroi...

  • Article
  • Open Access
6 Citations
4,776 Views
16 Pages

Peribacillus sp. AS_2, a leaf endophytic bacterium isolated from the medicinal plant Alectra sessiliflora, was previously identified using the 16S rRNA gene sequence. The draft genome of AS_2 had a 5,482,853 bp draft circular chromosome, 43 contigs,...

  • Article
  • Open Access
6 Citations
2,593 Views
17 Pages

Biodegradation of Free Cyanide by a New Isolated Alkaliphilic Bacillus licheniformis Strain

  • Daniel Uribe-Ramírez,
  • Eliseo Cristiani-Urbina and
  • Liliana Morales-Barrera

Microbial treatment of free-cyanide-polluted wastewater is a cost-effective, efficient, and eco-friendly method. Free-cyanide-degrading microbial cultures were isolated from different sources using batch-enrichment culture techniques, with acetate as...

  • Article
  • Open Access
11 Citations
3,501 Views
13 Pages

Properties of Potential Plant-Growth-Promoting Bacteria and Their Effect on Wheat Growth Promotion (Triticum aestivum) and Soil Characteristics

  • Elena Voronina,
  • Ekaterina Sokolova,
  • Irina Tromenschleger,
  • Olga Mishukova,
  • Inna Hlistun,
  • Matvei Miroshnik,
  • Oleg Savenkov,
  • Maria Buyanova,
  • Ilya Ivanov and
  • Natalya Smirnova
  • + 1 author

Plant-growth-promoting bacteria are an important economic and environmental resource as biofertilizers that can stimulate plant growth and improve agricultural yields. In this study, potential plant growth-promoting bacteria were isolated from soil s...

  • Article
  • Open Access
4 Citations
2,798 Views
19 Pages

Genome-Wide Identification of Cotton MicroRNAs Predicted for Targeting Cotton Leaf Curl Kokhran Virus-Lucknow

  • Muhammad Aleem Ashraf,
  • Judith K. Brown,
  • Muhammad Shahzad Iqbal and
  • Naitong Yu

Cotton leaf curl Kokhran virus (CLCuKoV) (genus, Begomovirus; family, Geminiviridae) is one of several plant virus pathogens of cotton (Gossypium hirsutum L.) that cause cotton leaf curl disease in Pakistan. Begomoviruses are transmitted by the white...

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Microbiol. Res. - ISSN 2036-7481