Biosensors, Volume 15, Issue 6

With the rapid development of modern molecular biology, microRNA (miRNA) has been demonstrated to be closely associated with the occurrence and development of tumors and holds significant promise as a biomarker for the early detection, diagnosis, and...

This study presents a mobile-based sperm analysis system featuring a user-friendly, droplet-loaded microfluidic chip that enables non-specialist users to perform the rapid and accurate quantitative evaluation of boar semen directly on the farm. The i...

Molecularly imprinted polymers (MIPs) provide selective, robust, and cost-effective platforms for the detection and removal of small toxic molecules in environmental, food, and biomedical contexts. This review offers a comprehensive overview of recen...

Myocardial infarction (MI) is a critical cardiovascular disease characterized by extensive myocardial necrosis occurring within a short timeframe. Traditional MI detection and localization techniques predominantly utilize single-domain features as in...

Microalbuminemia, characterized by a urinary albumin concentration between 20 and 200 mg/L, is a critical marker in assessing the risk of chronic kidney disease (CKD), diabetic nephropathy, and various other chronic conditions. Previously, we develop...

The COVID-19 pandemic highlighted the need for rapid, cost-effective tools to monitor transmission and immune response. We developed two novel paper-based colorimetric biosensors using glutaraldehyde as a protein dye—its first use in this conte...

The deformability of cells reflects their capacity for shape changes under external forces; however, the systematic investigation of deformation-influencing factors remains conspicuously underdeveloped. In this work, by using an incompressible neo-Ho...

This work presents a novel microfabrication process that addresses the interference of thiol groups on off-stoichiometry thiolene (OSTE) surfaces with the curing of polydimethylsiloxane (PDMS) by integrating the high-performance polymer Parylene-C. T...

Norovirus (NoV), a leading cause of acute gastroenteritis worldwide, imposes significant morbidity and economic burdens across all age groups. Timely and accurate laboratory diagnosis is crucial for effective outbreak control and patient management....

Breast cancer is a leading cause of cancer-related mortality worldwide, requiring efficient diagnostic tools for early detection and monitoring. Human epidermal growth factor receptor 2 (HER2) is a key biomarker for breast cancer classification, typi...

A novel nanocomposite based on electrochemically reduced graphene oxide (ERGO) and electropolymerized polyproflavine (PPFL) was obtained within a “one-pot” synthesis from natural deep eutectic solvent (NADES). NADES consisted of citric ac...

We are reporting the development of a high-performance, non-enzymatic electrochemical biosensor for selective lactate detection, integrating laser-induced graphene (LIG), gold nanoparticles (AuNPs), and a molecularly imprinted polymer (MIP) synthesiz...

Developing appropriate methods for real-time in situ investigation of how drugs influence signaling pathways and related biomolecules holds enormous potential for helping to provide an understanding of how anticancer drugs exert their effects. Herein...

Microfluidic modulation spectroscopy-infrared (MMS) offers a label-free, high-sensitivity approach for quantifying changes in protein secondary structures under native solution conditions. MMS subtracts the solvent backgrounds from sample signals by...

Lower extremity lymphedema (LEL) requires precise limb measurements for treatment evaluation and compression garment design. Tape measurement (TM) is the standard method but is time-consuming. Smartphones with light detection and ranging (LiDAR) tech...

Conventional blood-based detection methods for biomarkers and analytes face significant limitations, including complex processing, variability in blood components, and the inability to provide continuous monitoring. These challenges hinder the early...

Viral infections impose a significant burden on global public health and the economy. This study examines the current state of CRISPR-Cas system research, focusing on their applications in viral detection and their evolution over recent years. A bibl...

The rapid and precise identification of multiple pathogens is critical for ensuring food safety, controlling epidemics, diagnosing diseases, and monitoring the environment. However, traditional detection methods are hindered by complex workflows, the...

Development of sensitive and convenient alkaline phosphatase (ALP) detection methods is of great significance for food analysis, biomedical applications, and clinical tests. In this work, a sensitive detection method for ALP was established based on...

Multimode immunoassays based on multiple response mechanisms have received great attention due to their capacity to effectively improve the accuracy and reliability of biosensing platforms. However, few strategies have been reported for triple-mode i...

Point-of-care testing (POCT) has garnered widespread attention due to its rapid, convenient, and efficient detection capabilities, particularly playing an increasingly pivotal role in medical diagnostics and significantly improving the efficiency and...

Quantitative electroencephalography (QEEG) has emerged as a promising tool for detecting Alzheimer’s disease (AD). Among QEEG measures, Multiscale Fuzzy Entropy (MFE) shows great potential in identifying AD-related changes in EEG complexity. Ho...

Our study develops two configurations of MoS₂ and graphene heterostructures—MoS₂ on graphene (MG) and graphene on MoS₂ (GM)—to investigate biomolecule sensing in field-effect transistor (FET) biosensors. Leveraging MoS₂ and graphene&rsquo...

The growing demand for low-cost biosensors has stimulated the study of new technologies and materials like molybdenum disulfide (MoS₂). Due to its electroconductive nature and high surface-to-volume ratio, it allows for the ultra-sensitive detection...

Background: Diabetic neuropathy, particularly in its autonomic form, is often underdiagnosed despite its clinical significance. Electrochemical skin conductance (ESC), measured by SUDOSCAN, offers a non-invasive way to assess the autonomic dysfunctio...

The manipulation of cells and bioparticles has garnered significant interest in the field of viscoelastic microfluidics, particularly regarding its capacity for single-stream focusing within a three-dimensional and simple microchannel structure. The...

Sub-wavelength metallic nanostructures allow the squeezing of light within nanoscale regions, called plasmonic hotspots. Squeezed near-field light has been demonstrated to detect, modulate, and generate light in more effective ways. The enhanced elec...

Terahertz (THz) biosensing faces critical challenges in balancing high sensitivity and broadband spectral coverage, particularly under miniaturized device constraints. Conventional quasi-bound states in the continuum (QBIC) metasurfaces achieve high...

Background: The influence of gait speed on lower-extremity coordination while walking in people with Parkinson’s disease (pwPD) is poorly understood. This study sought to investigate the relationship between gait speed and hip–knee coordi...

Bioelectronic medicines record biological signals and provide electrical stimulation for the treatment of diseases. Advanced bioelectronic therapies require the development of electrodes that match the softness of the implanted tissues, as the presen...

This study investigated the effect of wearable ultrasound-imaging-based visual feedback (UVF) on assisting paretic ankle dorsiflexion training of chronic stroke survivors. Thirty-three participants with unilateral hemiplegia performed maximal isometr...

Prostate cancer is the second leading cause of cancer-related deaths among men in the United States. The early detection of aggressive forms is critical. Current diagnostic methods, including PSA testing and biopsies, are invasive and often yield fal...

Background: Food safety remains a global concern due to biological and chemical contaminants, including adulterants, pathogens, antibiotic residues, and pesticides. Traditional detection methods are accurate but limited by time requirements, complex...

Colorimetric-based biosensors are practical detection devices that can detect the presence and concentration of biomarkers through simple color changes. Conventional laboratory-based tests are highly sensitive but require long processing times and ex...

Zearalenone (ZEA), a potent mycotoxin commonly found in contaminated grains, presents a serious threat to food safety and public health. Conventional detection methods, including culture-based assays and laboratory-bound analytical tools, are often t...

Nucleic acid detection technology is crucial for molecular diagnosis. The advent of CRISPR/Cas12a-based nucleic acid detection has considerably broadened its scope, from the identification of infectious disease-causing microorganisms to the detection...

SPR biosensors operate on the principle of evanescent wave propagation at metal–dielectric interfaces in total internal reflection conditions, with consequent photonic energy attenuation. This plasmonic excitation occurs in specific conditions...

Background: Chemosensation in ticks opens a novel and unique field for scientific research. This study highlights ticks’ chemosensory system to comprehend its host-searching behavior and other integrated chemistry and biology involving Haller&r...

Histopathological staining remains the fibrosis diagnostic gold standard yet suffers from staining artifacts and variability. Nonlinear optical techniques (e.g., spontaneous fluorescence, Second Harmonic Generation) enhance accuracy but struggle with...

This research study proposes a heuristic hybrid deep neural network (DNN) gait sub-phase recognition model based on multi-source heterogeneous motion data fusion which quantifies gait phases and is applied in balance disorder rehabilitation control,...

Hydrogels are three-dimensional network structures composed of hydrophilic polymers that can swell in water and are very similar to soft tissues such as connective tissue or the extracellular matrix. DNA hydrogels are particularly notable for biomedi...

Nanoquenchers with a single quenching cofactor exhibit limited fluorescence quenching efficiency. In this work, a metal–organic hybrid with dual quenching cofactors (Cu²⁺ and pyrroloquinoline quinone or PQQ) was prepared by metal-coordinated as...

Norovirus, a foodborne pathogen, causes a significant economic and health burden globally. Although detection methods exist, they are expensive and non-field deployable. A flow-based dielectrophoretic biosensor was designed for the detection of foodb...

Identifying parathyroid glands during surgery is challenging and time-consuming due to their small size (3–5 mm) and camouflaged appearance in the background of the thyroid, lymph nodes, fat, and other neck structures. For the gland itself, it...

Quantifying inflammation plays a critical role in understanding the progression and development of various diseases. Non-invasive or minimally invasive wearable biosensors have garnered significant attention in recent years due to their convenience,...

The efficient and non-invasive collection of biological samples has become a critical challenge for the continued development of surface-enhanced Raman scattering (SERS). When integrated with minimally invasive microneedle (MN) sampling technology, S...

Betalains are hydrophilic natural pigments commonly found in plants of the Caryophyllales order, as well as in specific species and genera of fungi, such as Hygrocybe, Hygrophorus, and Amanita muscaria. Betalains are sorted into two groups: betacyani...

Micron-sized, ultrathin metal–organic framework (MOF) sheet is a two-dimensional (2D) hybrid material with a large specific surface area, which can be used not only in the fields of energy and biomedicine, but also in electrode modification to...

Understanding the tensile properties of extraocular muscles (EOMs) is crucial for successful strabismus surgery and accurate predictions of surgical outcomes. Assessments of EOM tensile strength are traditionally highly dependent on the expertise of...

CRISPR-Cas proteins from bacteria are powerful tools for gene editing and molecular diagnostics. Expanding capacity of CRISPR to low cost, multiplexed assays of biomarkers is a key to future disease diagnostics, since multiple biomarker detection is...