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Biosensors
Special Issues
Biosensing and Diagnosis—2nd Edition
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Biosensing and Diagnosis—2nd Edition

A special issue of Biosensors (ISSN 2079-6374). This special issue belongs to the section "Biosensors and Healthcare".

Deadline for manuscript submissions: 20 December 2025 | Viewed by 2173

Special Issue Editor

Dr. Jiyang Liu

E-Mail Website
Guest Editor
School of Chemistry and Chemical Engineering, Zhejiang Sci-Tech University, Hangzhou 310018, China
Interests: chemo-/bio-sensors; electrochemistry; electrochemiluminescence; bioanalysis; food analysis; environmental monitoring; noble metal nanomaterials; porous materials; carbon nanomaterials
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Recently, with the development of more biomarkers and new technologies, various sensing and diagnosis platforms have been proposed that are attractive because they are rapid, simple, inexpensive, portable, and not limited to a specific place. This can help to bring about more accurate diagnoses of health conditions, food and environmental monitoring, emergency security, and interpretations of complex results in real-world conditions, especially at the point of care, when an immediate decision on treatment most needs to be made. Undoubtedly, multidisciplinary collaborations will remain the focus of the future development of biosensing and diagnosis science, and low-cost and high-performance fabrication technology will further stimulate research interest from industry and academia. It is believed that more sensing products will be applied in real-life contexts from the laboratory, as their affordability, portability, user-friendliness, and accuracy meet the requirements for market application. In particular, by combining machine learning, big data analytics, and Internet of Things technology, integrated and intelligent sensors will shape the future of healthcare at a global level by providing users with more accurate diagnostics and more effective treatments. Accordingly, this Special Issue is devoted to a collection of significant advances on innovative research of biosensors, biosensing devices and systems, and their various applications.

Dr. Jiyang Liu
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Biosensors is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2200 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • point-of-care testing
  • biosensing device
  • biochemical analysis
  • diagnostics
  • biosensor
  • biosensing
  • bioimaging

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Published Papers (4 papers)

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Research

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16 pages, 2711 KiB  
Article
Ultrasensitive Peptide-Based Electrochemical Biosensor for Universal Diagnostic of Dengue
by Isis Campos Prado, João Pedro Rangel da Silva Carvalho, André Souza Araujo, Paloma Napoleão-Pêgo and Salvatore Giovanni De-Simone
Biosensors 2025, 15(4), 236; https://doi.org/10.3390/bios15040236 - 8 Apr 2025
Viewed by 495
Abstract
Dengue is a neglected disease mainly affecting tropical and subtropical countries. The diagnosis of dengue fever is still a problem since most of it is made from whole or recombinant DENV proteins, which present cross-reactions with other members of the Flavivirus family. Therefore, [...] Read more.
Dengue is a neglected disease mainly affecting tropical and subtropical countries. The diagnosis of dengue fever is still a problem since most of it is made from whole or recombinant DENV proteins, which present cross-reactions with other members of the Flavivirus family. Therefore, there is still a huge demand for new diagnostic methods that provide rapid, low-cost, easy-to-use confirmation. Thus, in this study, we developed an affordable electrochemical biosensor for rapidly detecting immunoglobulin G (IgG) serological antibodies in the sera of DENV-infected patients. An identified linear B-cell epitope (DENV/18) specific for DENV 1–4 serotypes recognized by IgG in patient sera was selected as a target molecule after a microarray of peptides using the SPOT-synthesis methodology. After chemical synthesis, the DENV/18-peptide was immobilized on the surface of the working electrode of a commercially available screen-printed gold electrode (SPGE). The capture of DENV-specific IgG allowed for the formation of an immunocomplex that was measured by cyclic voltammetry (CV) and differential pulse voltammetry (DPV) using a potassium ferrocyanide/ferricyanide ([Fe(CN)6]3−/4−) electrochemical probe. An evaluation of the biosensor’s performance showed a detection limit of 100 µg mL−1 for the synthetic peptides (DENV/18) and 1.21 ng mL−1 in CV and 0.43 ng mL−1 in DPV for human serum, with a sensitivity of 7.21 µA in CV and 8.79 µA in DPV. The differentiation of infected and uninfected individuals was possible even at a high dilution factor that reduced the required sample volumes to a few microliters. The final device proved suitable for diagnosing DENV by analyzing real serum samples, and the results showed good agreement with molecular biology diagnostics. The flexibility to conjugate other antigenic peptides to SPEs suggests that this technology could be rapidly adapted to diagnose other pathogens. Full article
(This article belongs to the Special Issue Biosensing and Diagnosis—2nd Edition)
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18 pages, 4136 KiB  
Article
Sensitive Detection of Biomarker in Gingival Crevicular Fluid Based on Enhanced Electrochemiluminescence by Nanochannel-Confined Co3O4 Nanocatalyst
by Changfeng Zhu, Yujiao Zhao and Jiyang Liu
Biosensors 2025, 15(1), 63; https://doi.org/10.3390/bios15010063 - 19 Jan 2025
Cited by 6 | Viewed by 1288
Abstract
The sensitive detection of inflammatory biomarkers in gingival crevicular fluid (GCF) is highly desirable for the evaluation of periodontal disease. Luminol-based electrochemiluminescence (ECL) immunosensors offer a promising approach for the fast and convenient detection of biomarkers. However, luminol’s low ECL efficiency under neutral [...] Read more.
The sensitive detection of inflammatory biomarkers in gingival crevicular fluid (GCF) is highly desirable for the evaluation of periodontal disease. Luminol-based electrochemiluminescence (ECL) immunosensors offer a promising approach for the fast and convenient detection of biomarkers. However, luminol’s low ECL efficiency under neutral conditions remains a challenge. This study developed an immunosensor by engineering an immunorecognition interface on the outer surface of mesoporous silica nanochannel film (SNF) and confining a Co3O4 nanocatalyst within the SNF nanochannels to improve the luminol ECL efficiency. The SNF was grown on an indium tin oxide (ITO) electrode using the simple Stöber solution growth method. A Co3O4 nanocatalyst was successfully confined within the SNF nanochannels through in situ electrodeposition, confirmed by X-ray photoelectron spectroscopy (XPS) and electrochemical measurements. The confined Co3O4 demonstrated excellent electrocatalytic activity, effectively enhancing luminol and H2O2 oxidation and boosting the ECL signal under neutral conditions. Using interleukin-6 (IL-6) as a proof-of-concept demonstration, the epoxy functionalization of the SNF outer surface enabled the covalent immobilization of capture antibodies, forming a specific immunorecognition interface. IL-6 binding induced immunocomplex formation, which reduced the ECL signal and allowed for quantitative detection. The immunosensor showed a linear detection range for IL-6 from 1 fg mL−1 to 10 ng mL−1, with a limit of detection (LOD) of 0.64 fg mL−1. It also demonstrated good selectivity and anti-interference capabilities, enabling the successful detection of IL-6 in artificial GCF samples. Full article
(This article belongs to the Special Issue Biosensing and Diagnosis—2nd Edition)
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Review

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20 pages, 5093 KiB  
Review
Convenient Biochemical Testing Technologies for Oral Disease Risk Warning: Opportunities and Challenges
by Ying Liu, Jincheng Xu, Siyuan Wang, Yuanfang Li, Li Ji, Dong Xie and Jianhua Zhou
Biosensors 2025, 15(5), 327; https://doi.org/10.3390/bios15050327 - 20 May 2025
Abstract
In recent years, attention toward oral health issues has increased with economic development and improvements in quality of life. Biochemical testing technologies offer an efficient method for identifying insidious pathological changes in the oral cavity. Frequent home-based self-screening can enable early identification of [...] Read more.
In recent years, attention toward oral health issues has increased with economic development and improvements in quality of life. Biochemical testing technologies offer an efficient method for identifying insidious pathological changes in the oral cavity. Frequent home-based self-screening can enable early identification of dental disease risks, thus facilitating timely interventions. Convenient home-based biochemical testing methods must be user-friendly, cost-effective, and operable without specialized equipment or extensive training. This review summarizes recent advances in convenient biochemical testing methods for the detection and diagnosis of oral diseases, focusing on their reliability, user compliance, and practicality for home-based applications. This review highlights the significance of biomarker distribution imaging for simultaneously identifying multiple lesions and provides perspectives on future research directions. By promoting interdisciplinary collaboration in biochemical diagnostics, this review outlines pathways toward personalized oral healthcare, precision dentistry, and enhanced overall health outcomes. Full article
(This article belongs to the Special Issue Biosensing and Diagnosis—2nd Edition)
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44 pages, 16366 KiB  
Review
Recent Advances in Paper-Based Electronics: Emphasis on Field-Effect Transistors and Sensors
by Dimitris Barmpakos, Apostolos Apostolakis, Fadi Jaber, Konstantinos Aidinis and Grigoris Kaltsas
Biosensors 2025, 15(5), 324; https://doi.org/10.3390/bios15050324 - 19 May 2025
Abstract
Paper-based electronics have emerged as a sustainable, low-cost, and flexible alternative to traditional substrates for electronics, particularly for disposable and wearable applications. This review outlines recent developments in paper-based devices, focusing on sensors and paper-based field-effect transistors (PFETs). Key fabrication techniques such as [...] Read more.
Paper-based electronics have emerged as a sustainable, low-cost, and flexible alternative to traditional substrates for electronics, particularly for disposable and wearable applications. This review outlines recent developments in paper-based devices, focusing on sensors and paper-based field-effect transistors (PFETs). Key fabrication techniques such as laser-induced graphene, inkjet printing, and screen printing have enabled the creation of highly sensitive and selective devices on various paper substrates. Material innovations, especially the integration of graphene, carbon-based materials, conductive polymers, and other novel micro- and nano-enabled materials, have significantly enhanced device performance. This review discusses modern applications of paper-based electronics, with a particular emphasis on biosensors, electrochemical and physical sensors, and PFETs designed for flexibility, low power, and high sensitivity. Advances in PFET architectures have further enabled the development of logic gates and memory systems on paper, highlighting the potential for fully integrated circuits. Despite challenges in durability and performance consistency, the field is rapidly evolving, driven by the demand for green electronics and the need for decentralized, point-of-care diagnostic tools. This paper also identifies detection strategies used in paper-based sensors, reviews limitations in the current fabrication methods, and outlines opportunities for the scalable production of multifunctional paper-based systems. This review addresses a critical gap in the literature by linking device-level innovation with real-world sensor applications on paper substrates. Full article
(This article belongs to the Special Issue Biosensing and Diagnosis—2nd Edition)
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