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Biosensors
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Advances in Nanozyme-Based Biosensors
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Advances in Nanozyme-Based Biosensors

A special issue of Biosensors (ISSN 2079-6374). This special issue belongs to the section "Biosensor Materials".

Deadline for manuscript submissions: 31 August 2026 | Viewed by 8405

Special Issue Editors

Dr. Jiaojiao Zhou

E-Mail Website
Guest Editor
School of Modern Industry for Selenium Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, China
Interests: food safety analysis; nanozymes; environmental analysis
Special Issues, Collections and Topics in MDPI journals
Dr. Xiangheng Niu

E-Mail Website
Guest Editor
School of Public Health, Hengyang Medical School, University of South China, Hengyang 421001, China
Interests: nanozymes; environmental detection; food analysis; biochemical sensing
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Nanozymes have great potential to be used as an alternative to natural enzymes in a variety of fields. The emerging applications of biosensors include analytical, environmental, and biomedical applications. Compared with natural enzymes, nanozymes have the merits of low cost, high stability, and large-scale production. Since the pioneering work by Yan and co-workers in 2007, nanozymes with diverse enzyme-like activities have been identified and are widely used in biosensing, biomedicine, and biotherapy.

For this Special Issue, we welcome original research papers as well as reviews of current developments in the design of nanozyme-based biosensors. This includes the design of state-of-the-art nanozyme-based biosensors for analytical, environmental, and biomedical applications. Theoretical research on nanozymes with high specific activity is also encouraged. The design and development of the catalytic mechanism of nanozymes, rational engineering of active sites, and portable/handheld nanozyme-based platforms for point-of-care applications are of particular interest. Reviews should provide an in-depth examination of the most recent research in a specific context or discuss existing and future issues related to the nanozyme field.

Dr. Jiaojiao Zhou
Dr. Xiangheng Niu
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 250 words) can be sent to the Editorial Office for assessment.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Biosensors is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2200 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • nanozymes
  • biosensor
  • bioimaging
  • nanozyme-based aptasensor
  • nanozyme-involved immunoassay
  • food analysis
  • environmental detection
  • nanozyme-based biochemical analysis

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Published Papers (4 papers)

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Research

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14 pages, 2232 KB  
Article
FGAN@PB NP Nanozyme-Based Colorimetric–Photothermal Dual-Mode Immunosensor for Malachite Green Detection
by Min-Fu Wu, Jing-Min Li, Sha Li, Min-Hua Wu, Ri-Sheng Chen, Yan-Can Liu, Jian-Nan Liu, Zhen-Lin Xu, Yi-Chao Yang, Jia-Dong Li, Qing-Yi Lei, Si-Min Zhan and Lin Luo
Biosensors 2025, 15(11), 719; https://doi.org/10.3390/bios15110719 - 30 Oct 2025
Viewed by 502
Abstract
In this study, a colorimetric–photothermal dual-mode immunosensor based on Fe(Ⅲ)–gallic acid composite Prussian blue nanozyme (FGAN@PB NPs) was developed for the highly sensitive detection of malachite green (MG) in aquatic products. This strategy addresses the stability limitations associated with conventional horseradish peroxidase (HRP). [...] Read more.
In this study, a colorimetric–photothermal dual-mode immunosensor based on Fe(Ⅲ)–gallic acid composite Prussian blue nanozyme (FGAN@PB NPs) was developed for the highly sensitive detection of malachite green (MG) in aquatic products. This strategy addresses the stability limitations associated with conventional horseradish peroxidase (HRP). In the colorimetric mode, the immunosensor exhibited an IC50 of 7.56 ng/mL with a linear detection range of 2.21–25.84 ng/mL. In the photothermal mode, the linear range was 0.262–25.6 ng/mL, with a detection limit (LOD) of 0.31 ng/mL. The results from the two detection modes were mutually corroborative. Moreover, the detection of the proposed immunosensor was strongly correlated with the LC-MS/MS, offering a promising approach for the rapid on-site screening of MG and improving its applicability in complex sample matrices. Full article
(This article belongs to the Special Issue Advances in Nanozyme-Based Biosensors)
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15 pages, 1974 KB  
Article
A Flexible Electrochemical Sensor Based on Porous Ceria Hollow Microspheres Nanozyme for Sensitive Detection of H2O2
by Jie Huang, Xuanda He, Shuang Zou, Keying Ling, Hongying Zhu, Qijia Jiang, Yuxuan Zhang, Zijian Feng, Penghui Wang, Xiaofei Duan, Haiyang Liao, Zheng Yuan, Yiwu Liu and Jinghua Tan
Biosensors 2025, 15(10), 664; https://doi.org/10.3390/bios15100664 - 2 Oct 2025
Viewed by 818
Abstract
The development of cost-effective and highly sensitive hydrogen peroxide (H2O2) biosensors with robust stability is critical due to the pivotal role of H2O2 in biological processes and its broad utility across various applications. In this work, [...] Read more.
The development of cost-effective and highly sensitive hydrogen peroxide (H2O2) biosensors with robust stability is critical due to the pivotal role of H2O2 in biological processes and its broad utility across various applications. In this work, porous ceria hollow microspheres (CeO2-phm) were synthesized using a solvothermal synthesis method and employed in the construction of an electrochemical biosensor for H2O2 detection. The resulting CeO2-phm featured a uniform pore size centered at 3.4 nm and a high specific surface area of 168.6 m2/g. These structural attributes contribute to an increased number of active catalytic sites and promote efficient electrolyte penetration and charge transport, thereby enhancing its electrochemical sensing performance. When integrated into screen-printed carbon electrodes (CeO2-phm/cMWCNTs/SPCE), the CeO2-phm/cMWCNTs/SPCE-based biosensor exhibited a wide linear detection range from 0.5 to 450 μM, a low detection limit of 0.017 μM, and a high sensitivity of 2070.9 and 2161.6 μA·mM−1·cm−2—surpassing the performance of many previously reported H2O2 sensors. In addition, the CeO2-phm/cMWCNTs/SPCE-based biosensor possesses excellent anti-interference performance, repeatability, reproducibility, and stability. Its effectiveness was further validated through successful application in real sample analysis. Hence, CeO2-phm with solvothermal synthesis has great potential applications as a sensing material for the quantitative determination of H2O2. Full article
(This article belongs to the Special Issue Advances in Nanozyme-Based Biosensors)
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Review

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27 pages, 2382 KB  
Review
Advances of Nanozyme-Driven Multimodal Sensing Strategies in Point-of-Care Testing
by Ziyi Chang, Qingjie Fu, Mengke Wang and Demin Duan
Biosensors 2025, 15(6), 375; https://doi.org/10.3390/bios15060375 - 10 Jun 2025
Cited by 5 | Viewed by 2939
Abstract
Point-of-care testing (POCT) has garnered widespread attention due to its rapid, convenient, and efficient detection capabilities, particularly playing an increasingly pivotal role in medical diagnostics and significantly improving the efficiency and quality of healthcare services. Nanozymes, as novel enzyme-mimicking materials, have emerged as [...] Read more.
Point-of-care testing (POCT) has garnered widespread attention due to its rapid, convenient, and efficient detection capabilities, particularly playing an increasingly pivotal role in medical diagnostics and significantly improving the efficiency and quality of healthcare services. Nanozymes, as novel enzyme-mimicking materials, have emerged as a research hotspot owing to their superior catalytic performance, low cost, and robust stability. This review provides a systematic overview of the fundamental characteristics and classifications of nanozymes, along with various sensing strategies employed in POCT applications, colorimetric, electrochemical, fluorescent, chemiluminescent, and surface-enhanced Raman scattering (SERS)-based approaches. Furthermore, this review highlights innovative designs that enhance the sensitivity and accuracy of POCT across multiple domains, such as biomarker detection, environmental monitoring, and food safety analysis, thereby offering novel perspectives for the practical implementation of nanozymes in point-of-care diagnostics. Finally, this review analyzes current challenges in nanozyme-based POCT systems, including limitations in optimizing catalytic activity, ensuring nanozyme homogeneity, and achieving large-scale production, while proposing future development trajectories. Full article
(This article belongs to the Special Issue Advances in Nanozyme-Based Biosensors)
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35 pages, 5385 KB  
Review
Exploring Nucleic Acid Nanozymes: A New Frontier in Biosensor Development
by Keren Chen, Zaihui Du, Yangzi Zhang, Ruobin Bai, Longjiao Zhu and Wentao Xu
Biosensors 2025, 15(3), 142; https://doi.org/10.3390/bios15030142 - 24 Feb 2025
Cited by 5 | Viewed by 3651
Abstract
With the growing interest in nucleic acids and nanozymes, nucleic acid nanozymes (NANs) have emerged as a promising alternative to traditional enzyme catalysts, combining the advantages of nucleic acids and nanomaterials, and are widely applied in the field of biosensing. This review provides [...] Read more.
With the growing interest in nucleic acids and nanozymes, nucleic acid nanozymes (NANs) have emerged as a promising alternative to traditional enzyme catalysts, combining the advantages of nucleic acids and nanomaterials, and are widely applied in the field of biosensing. This review provides a comprehensive overview of recent studies on NAN-based biosensors. It classifies NANs based on six distinct enzymatic activities: peroxidase-like, oxidase-like, catalase-like, superoxide dismutase-like, laccase-like, and glucose oxidase-like. This review emphasizes how the catalytic activity of nanozymes is significantly influenced by the properties of nucleic acids and explores the regulatory mechanisms governing the catalytic activity of NANs. Additionally, it systematically reviews important research progress on NANs in colorimetric, fluorescent, electrochemical, SERS, and chemiluminescent sensors, offering insights into the development of the NAN field and biosensor applications. Full article
(This article belongs to the Special Issue Advances in Nanozyme-Based Biosensors)
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