Skip to Content

44 Results Found

  • Review
  • Open Access
431 Views
19 Pages

3 March 2026

Veterinary forensic pathology is an evolving discipline at the intersection of veterinary medicine, forensic science, and animal welfare law. This article underscores the growing importance of this field in advancing both animal welfare and justice....

  • Article
  • Open Access
11 Citations
3,530 Views
11 Pages

Evaluation of Muscle Proteins for Estimating the Post-Mortem Interval in Veterinary Forensic Pathology

  • Giuseppe Piegari,
  • Valeria De Pasquale,
  • Ilaria d’Aquino,
  • Davide De Biase,
  • Giulia Caccia,
  • Carlo Pietro Campobasso,
  • Simona Tafuri,
  • Valeria Russo and
  • Orlando Paciello

6 February 2023

Postmortem cadaveric changes are commonly used to estimate the postmortem interval (PMI) in humans and animals. However, these modifications have been poorly investigated in animals of interest to veterinary forensic pathology. The aim of this study...

  • Feature Paper
  • Article
  • Open Access
1 Citations
3,114 Views
15 Pages

Pathological Changes and Sodium Rhodizonate Test as Tools for Investigating Gunshot Wounds in Veterinary Forensic Pathology

  • Giuseppe Piegari,
  • Ilaria d’Aquino,
  • Giovanni Valerio Salanti,
  • Vittoria Romano,
  • Gianluca Miletti,
  • Emanuela Sannino,
  • Evaristo Di Napoli,
  • Lorenzo Riccio,
  • Davide De Biase and
  • Orlando Paciello

9 October 2024

Gunshot wound morphology and gunshot residues (GSRs) evaluation have been poorly investigated in veterinary forensic pathology. The aims of the present study were to assess the gunshot wound morphology in animals and evaluate the detectability of lea...

  • Communication
  • Open Access
464 Views
9 Pages

11 December 2025

In forensic veterinary investigations of suspicious animal deaths involving loss of body parts, it is essential to determine whether the wounds were caused by human intervention or by other animals. Detailed postmortem examination of the wounds, comb...

  • Article
  • Open Access
278 Views
12 Pages

27 February 2026

Pregabalin, as the active ingredient of various medications, is used in humans to treat generalized anxiety disorder (GAD), epilepsy, fibromyalgia, and neuropathic pain. In animals, pregabalin is used as an anesthetic. The aim of the present study wa...

  • Article
  • Open Access
1 Citations
2,327 Views
17 Pages

Evolution of Suspected Cat Abuse Between 2020 and 2024 in the Community of Madrid (Spain)

  • Nicolás Aradilla,
  • Javier María De Pablo-Moreno,
  • Néstor Porras,
  • Blanca Chinchilla and
  • Antonio Rodríguez-Bertos

3 October 2025

Despite the well-established link between animal abuse and interpersonal violence, animal mistreatment remains a global issue. Challenges such as inconsistent legislation, limited training of specialized pathologists, and lack of regional data persis...

  • Article
  • Open Access
1 Citations
3,226 Views
8 Pages

23 December 2022

The Northern Bald Ibis Geronticus eremita is an endangered species, and recently it was reintroduced in Europe by the Waldrappteam. The reintroduction program has been strongly threatened by several sudden deaths, mainly in Italy. The present study u...

  • Review
  • Open Access
15 Citations
10,256 Views
30 Pages

10 August 2022

Veterinary forensics is becoming more important in our society as a result of the growing demand for investigations related to crimes against animals or investigations of criminal deaths caused by animals. A veterinarian may participate as an expert...

  • Article
  • Open Access
1 Citations
3,314 Views
18 Pages

On-Site Inspection Form in Veterinary Cases: The Parma Veterinary Form

  • Cristina Marchetti,
  • Luigi Mastrogiuseppe,
  • Stefano Vanin,
  • Rossana Cecchi and
  • Mirella Gherardi

22 June 2023

The on-site inspection of the scene of an animal cadaver is crucial for a correct interpretation of the autopsy results, to determine the manner, method, and cause of death. This information plays a crucial role in the control of public health includ...

  • Article
  • Open Access
10 Citations
5,887 Views
14 Pages

Characterization of Suspected Crimes against Companion Animals in Portugal

  • Diana Araújo,
  • Carla Lima,
  • João R. Mesquita,
  • Irina Amorim and
  • Cristina Ochôa

20 September 2021

Animal crimes are a widespread phenomenon with serious implications for animal welfare, individual well-being and for society in general. These crimes are universal and represent a major problem in human/animal interaction. In Portugal, current law 6...

  • Article
  • Open Access
1,141 Views
12 Pages

Background: The aging of fly larvae is primarily determined by their temperature-dependent growth rates, a concept widely applied in forensic entomology to estimate the minimum postmortem interval using the accumulated degree day/hour (ADD/ADH) metho...

  • Article
  • Open Access
1 Citations
5,807 Views
14 Pages

Agonal Thrombus at Necropsy—A Third Category of Blood Coagulation in Domestic Carnivores

  • Raluca Elena Tiu,
  • Raluca Ioana Rizac,
  • George Laurentiu Nicolae,
  • Raluca Mihaela Turbatu and
  • Emilia Ciobotaru-Pirvu

30 August 2023

Agonal thrombus is infrequently discussed in veterinary forensic pathology, being misdiagnosed as postmortem coagulation. The main purpose of the present study is to confirm that agonal thrombosis is an important tool in ruling out sudden death and t...

  • Case Report
  • Open Access
1,510 Views
13 Pages

Characteristics of Three Forensic Veterinary Cases Involving Pet Deaths in Domestic Murder-Suicide Incidents

  • Yuko Kihara,
  • Yohsuke Makino,
  • Suguru Torimitsu,
  • Fumiko Chiba,
  • Hirotaro Iwase,
  • Makoto Nakajima and
  • Aki Tanaka

26 August 2025

Murder-suicide, also referred to as homicide-suicide, results in multiple casualties. The victims are typically human, and cases involving the inclusion of pets, sometimes termed “murder-suicide-peticide”, have been rarely reported worldw...

  • Article
  • Open Access
1 Citations
1,635 Views
19 Pages

16 August 2025

In this study, bone detection was performed using the YOLO algorithm on a dataset comprising photographs of the scapula, humerus, and femur from cattle, horses, and dogs. Subsequently, convolutional neural networks (CNNs) were employed to classify bo...

  • Article
  • Open Access
2 Citations
2,092 Views
9 Pages

Morphometrical and Immunohistochemical Evaluation of Kidney as an Indirect Parameter to Estimate Age in Puppies in Veterinary Forensic Pathology

  • Ilaria d’Aquino,
  • Giuseppe Piegari,
  • Gianluca Miletti,
  • Emanuela Sannino,
  • Dario Costanza,
  • Leonardo Meomartino,
  • Rosario Fico,
  • Lorenzo Riccio,
  • Emanuela Vaccaro and
  • Orlando Paciello
  • + 1 author

18 August 2023

Estimation of age represents a central focus in the veterinary forensic pathology field. Currently, the visual examination of the dentition and the skeletal age are the main methods to estimate the age of puppies. Nevertheless, these methods are affe...

  • Article
  • Open Access
8 Citations
14,770 Views
16 Pages

Forensic Death Investigations of Dog Bite Injuries in 31 Cats

  • Chia-Lin Hsiou,
  • Chih-Chin Hsu,
  • Pei-Wen Liao,
  • Fu-Hua Yang,
  • Ann Nee Lee and
  • Wei-Hsiang Huang

13 September 2022

Animal bite injuries are common in free-ranging cats in Taiwan, and most fatal animal bite events are presumed to be caused by dogs. However, speculation regarding animal abuse may occur when carcasses with prominent injuries are found by members of...

  • Technical Note
  • Open Access
3,285 Views
14 Pages

4 January 2024

Lucilia silvarum Meigen (Diptera: Calliphoridae) is widespread throughout North America and Europe. Described in 1826, this blow fly was quickly associated with myiasis in amphibians, and to date has rarely been reported in carrion. There is limited...

  • Article
  • Open Access
15 Citations
18,308 Views
15 Pages

Relationship Between Scarring and Dog Aggression in Pit Bull-Type Dogs Involved in Organized Dogfighting

  • Katherine A. Miller,
  • Rachel Touroo,
  • C. Victor Spain,
  • Kelly Jones,
  • Pamela Reid and
  • Randall Lockwood

15 November 2016

When pit bull-type dogs are seized in an investigation of organized dogfighting, heavily scarred dogs are often assumed to be highly dog aggressive due to a history of fighting. These dogs may be deemed dangerous and euthanized based on scarring alon...

  • Feature Paper
  • Article
  • Open Access
5 Citations
3,984 Views
20 Pages

Morphological and Immunohistochemical Changes in Progressive Postmortem Autolysis of the Murine Brain

  • Francesca Parisi,
  • Sara Degl’Innocenti,
  • Çağla Aytaş,
  • Andrea Pirone and
  • Carlo Cantile

20 December 2024

In this time series study, the temporal sequences of postmortem changes in brains kept at different temperatures were investigated in different areas of mouse brains. Fixation of tissues kept at different storage temperatures (4 °C, 22 °C, 37 °C) was...

  • Article
  • Open Access
2 Citations
4,899 Views
23 Pages

Preliminary Considerations for Crime Scene Analysis in Cases of Animals Affected by Homemade Ammonium Nitrate and Aluminum Powder Anti-Personnel Landmines in Colombia: Characteristics and Effects

  • Carlos Jaramillo Gutiérrez,
  • Gustavo Farías Roldán,
  • Krešimir Severin,
  • Ubicelio Martin Orozco,
  • Pilar Marín García and
  • Víctor Toledo González

29 July 2022

During the armed conflict in Colombia, homemade improvised antipersonnel landmines were used to neutralize the adversary. Many active artifacts remain buried, causing damage to biodiversity by exploding. The extensive literature describes the effects...

  • Feature Paper
  • Article
  • Open Access
10 Citations
4,611 Views
18 Pages

An Easy Procedure to Quantify Anticoagulant Rodenticides and Pharmaceutical Active Compounds in Soils

  • Andrea Acosta-Dacal,
  • Cristian Rial-Berriel,
  • Ricardo Díaz-Díaz,
  • María del Mar Bernal-Suárez,
  • Manuel Zumbado,
  • Luis Alberto Henríquez-Hernández and
  • Octavio P. Luzardo

10 April 2021

A modified QuEChERS (quick, easy, cheap, effective, rugged, and safe) extraction was validated for the extraction of seven coumarin anticoagulant rodenticides (ARs) and 36 pharmaceutical active compounds (PhACs) residues in soil samples using liquid...

  • Systematic Review
  • Open Access
4 Citations
3,599 Views
18 Pages

Characterization of the Variables Related to Reports of Death Due to Canine Bites in Scientific Articles during the Years 2013–2017: A Systematic Review

  • Carmen Luz Barrios,
  • Valentina Aguirre-Olea,
  • Carlos Bustos-López,
  • Sandra Pérez-Vergara and
  • Sandra Claros-Alegría

9 September 2021

Canine bites are an important public health problem, with consequences such as physical injuries, psychological trauma, transmission of zoonoses, infections and they can even cause death. To avoid deaths caused by this type of bite, multiple factors...

  • Article
  • Open Access
6 Citations
5,531 Views
12 Pages

Pathological, Bacteriological and Virological Findings in Sudden and Unexpected Deaths in Young Dogs

  • Giuseppe Piegari,
  • Lorena Cardillo,
  • Flora Alfano,
  • Lucia Vangone,
  • Valentina Iovane and
  • Giovanna Fusco

3 July 2020

In human medicine, “sudden death” has been defined by the World Health Organization (WHO) as a non-violent, unexpected death occurring less than 24 h from the onset of symptoms. The aims of this study were: (1) to estimate the proportiona...

  • Article
  • Open Access
8 Citations
5,958 Views
12 Pages

6 May 2020

Due to their professional abilities, veterinarians have a duty to reduce animal abuse. Therefore, it is crucial to understand their attitude and behavior on encountering animal abuse cases. We analyzed the responses from completed questionnaires (n =...

  • Article
  • Open Access
12 Citations
12,155 Views
20 Pages

A Morphological and Morphometric Dental Analysis as a Forensic Tool to Identify the Iberian Wolf (Canis Lupus Signatus)

  • Víctor Toledo González,
  • Fernando Ortega Ojeda,
  • Gabriel M. Fonseca,
  • Carmen García-Ruiz,
  • Pablo Navarro Cáceres,
  • Pilar Pérez-Lloret and
  • María del Pilar Marín García

3 June 2020

Depredation by the Iberian wolf (Canis lupus signatus) is currently thought to be a problem in some areas of Spain. However, there are few technically validated forensic tools available to determine the veracity of claims with a high degree of scient...

  • Article
  • Open Access
8 Citations
3,615 Views
8 Pages

19 October 2019

Strychnine is an alkaloid with strong toxic properties. Poisoning results in muscular contractions and death through asphyxiation. Intentional or accidental poisonings with strychnine occur mainly in small animals, especially dogs and occasionally ca...

  • Article
  • Open Access
344 Views
11 Pages

Time-Since-Deposition Signatures for Canine Blood Based on Cellular Autofluorescence

  • Alysia Townsley,
  • Gabrielle Wolfe,
  • Madison Smith,
  • Arianna DeCorte,
  • Amanda Elswick Gentry and
  • Christopher J. Ehrhardt

10 December 2025

Animal cruelty investigations can be hampered by not knowing when a biological sample was deposited at a crime scene, i.e., the sample’s time-since-deposition (TSD). The goal of this study was to characterize a new signature system for estimati...

  • Case Report
  • Open Access
550 Views
7 Pages

14 December 2025

Dog bite incidents represent a significant public health concern, and DNA analysis, in addition to morphological comparison, has been recognized as a useful tool for identifying the offending dog. STR typing has been established as a reliable method...

  • Article
  • Open Access
428 Views
18 Pages

LC-QTOF-MS as a Tool for Quantitative and Qualitative Analysis of Isoniazid and Its Metabolites in Dog Liver Samples

  • Julia Horla,
  • Paweł Jajor,
  • Tetiana Holumbiiovska,
  • Mykola Zhyla,
  • Nataliia Vretsona,
  • Galyna Kotsyumbas and
  • Błażej Poźniak

13 February 2026

Isoniazid (INH) is an antitubercular drug that exhibits high toxicity in dogs due to the absence of N-acetyltransferase activity in this species. Consequently, it has been implicated in both accidental and intentional poisonings in dogs. The aim of t...

  • Case Report
  • Open Access
939 Views
11 Pages

18 June 2025

A series of incidents involving damage to vehicle speed sensor cables occurred in an urban area in Japan. At the request of the police, DNA analysis was conducted to identify the animal species responsible. Swab samples collected from the damaged sec...

  • Case Report
  • Open Access
2 Citations
5,785 Views
9 Pages

A female dog had allegedly been alive one day before its death. The veterinary office thought about accusing the dog’s owner for animal cruelty and commissioned forensic entomological expertise for the calculation of the time of insect coloniza...

  • Article
  • Open Access
1 Citations
1,736 Views
17 Pages

Ecological Preferences of Calliphoridae and Sarcophagidae (Diptera) in the Region Calabria (Southern Italy)

  • Domenico Bonelli,
  • Federica Mendicino,
  • Francesco Carlomagno,
  • Giuseppe Luzzi,
  • Antonino Siclari,
  • Federica Fumo,
  • Erica Di Biase,
  • Michele Mistri,
  • Daniel Whitmore and
  • Teresa Bonacci
  • + 2 authors

25 August 2025

Diptera belonging to the families Calliphoridae and Sarcophagidae are known for their diversified trophic preferences and for their forensic and medical-veterinary relevance. The ecological preferences (distribution, abundance and habitat) of these t...

  • Article
  • Open Access
2 Citations
9,565 Views
12 Pages

Feline Forensics: Revealing the Unique Decomposition of Cats

  • Kelly Bagsby,
  • Adam W. Stern and
  • Krystal R. Hans

24 March 2024

Limited data about the differences between the decomposition of animals with fur exist within the forensic veterinary medicine discipline. Due to the sheer number of animals used as animal models and the number of animals that exist, determining stag...

  • Article
  • Open Access
6 Citations
6,875 Views
27 Pages

Ageing Effects in Mounting Media of Microscope Slide Samples from Natural History Collections: A Case Study with Canada Balsam and PermountTM

  • Thomas Schmid,
  • Julia Hidde,
  • Sophie Grünier,
  • Robert Jungnickel,
  • Petra Dariz,
  • Jens Riedel and
  • Birger Neuhaus

27 June 2021

Microscope slide collections represent extremely valuable depositories of research material in a natural history, forensic, veterinary, and medical context. Unfortunately, most mounting media of these slides deteriorate over time, with the reason for...

  • Article
  • Open Access
5 Citations
3,350 Views
15 Pages

Comparative Mitogenomics of Flesh Flies: Implications for Phylogeny

  • Jin Shang,
  • Wentian Xu,
  • Xiaofang Huang,
  • Dong Zhang,
  • Liping Yan and
  • Thomas Pape

10 August 2022

Flesh flies (Diptera: Sarcophagidae) represent a rapid radiation belonging to the Calyptratae. With more than 3000 known species, they are extraordinarily diverse in terms of their breeding habits and are therefore of particular importance in human a...

  • Article
  • Open Access
6 Citations
3,113 Views
13 Pages

A Biochemical and Histological Assessment of Postmortem Changes to the Eyes of Domestic Pigs: A Preliminary Study

  • Magdalena Palić,
  • Ivan-Conrado Šoštarić Zuckermann,
  • Petar Džaja,
  • Blanka Beer Ljubić and
  • Krešimir Severin

15 April 2024

The Postmortem Interval (PMI) is the time from the death of an animal to its discovery. From a veterinary forensic standpoint, an accurate estimation of the PMI is of particular importance, especially with the observed increase in deaths of domestic...

  • Article
  • Open Access
4 Citations
3,152 Views
10 Pages

Abnormal Prothrombin (PIVKA-II) Expression in Canine Tissues as an Indicator of Anticoagulant Poisoning

  • Lorella Maniscalco,
  • Katia Varello,
  • Simona Zoppi,
  • Giuseppina Abbamonte,
  • Marta Ferrero,
  • Elena Torres,
  • Federica Ostorero,
  • Francesca Rossi and
  • Elena Bozzetta

6 September 2021

PIVKA-II is an aberrant form of vitamin K that has been demonstrated to be increased in human coagulation disorders and in some neoplastic diseases. In veterinary medicine, PIVKA-II levels have been demonstrated to be useful for distinguishing antico...

  • Review
  • Open Access
2,141 Views
21 Pages

9 December 2025

Quantitative Polymerase Chain Reaction (qPCR) is a very sensitive method to determine small amounts of DNA or RNA in experimental, environmental, veterinary, forensic and clinical samples. Despite efforts from the qPCR community to address qPCR varia...

  • Review
  • Open Access
457 Views
15 Pages

The Emergence of Fentanyl + Medetomidine Overdose: Pharmacology, Toxicology, and Need for Poly-Drug Reversal Therapeutics

  • Robert B. Raffa,
  • Eugene Vortsman,
  • Joseph V. Pergolizzi,
  • Krista Casazza and
  • Morgan King

The overdose mortality landscape has shifted from predominantly opioid exposures to a polysubstance epidemic increasingly driven by illicit fentanyl and fentanyl analogs combined with other centrally active agents. Among the co-intoxicants, veterinar...

  • Article
  • Open Access
1 Citations
2,391 Views
38 Pages

16 January 2025

Calliphoridae, or blow flies, are of much ecological and practical importance given their roles in decompositional ecology, medical and veterinary myiasis, and forensic entomology. As ephemeral and rapidly developing species, adults are frequently no...

  • Article
  • Open Access
4 Citations
3,567 Views
15 Pages

Fouling-Resistant Voltammetric Xylazine Sensors for Detection of the Street Drug “Tranq”

  • Joyce E. Stern,
  • Ann H. Wemple,
  • Charles W. Sheppard,
  • Arielle Vinnikov and
  • Michael C. Leopold

30 October 2024

As the opioid crisis continues to wreak havoc on a global scale, it is increasingly critical to develop methodologies to detect the most dangerous drugs such as fentanyl and its derivatives, which have orders of magnitude higher potency than morphine...

  • Article
  • Open Access
4 Citations
2,255 Views
14 Pages

27 June 2024

The present study investigates the developmental process of Chrysomya rufifacies (Macquart) pupae and their dependency on soil composition, moisture levels, and temperature changes. This research holds implications for forensic and veterinary applica...

  • Article
  • Open Access
1,598 Views
22 Pages

Adsorptive Cathodic Stripping Analysis of Xylazine Within Fouling-Resistant and Nanomaterial-Enhanced Modified Electrode Sensors

  • Michael C. Leopold,
  • Charles W. Sheppard,
  • Joyce E. Stern,
  • Arielle Vinnikov,
  • Ann H. Wemple and
  • Ben H. Edelman

26 August 2025

Xylazine (XYL), an FDA-approved veterinary tranquilizer, is being abused both as an opioid adulterant in a street-drug known as “Tranq-dope” and as a date rape drug. Given its now nearly ubiquitous use with fentanyl and fentanyl derivativ...