You are currently on the new version of our website. Access the old version .

584 Results Found

  • Review
  • Open Access
23 Citations
7,732 Views
14 Pages

Metabolomics of Non-Saccharomyces Yeasts in Fermented Beverages

  • Daniel J. Ellis,
  • Edward D. Kerr,
  • Gerhard Schenk and
  • Benjamin L. Schulz

Fermented beverages have been consumed for millennia and today support a global industry producing diverse products. Saccharomyces yeasts currently dominate the fermented beverage industry, but consumer demands for alternative products with a variety...

  • Article
  • Open Access
18 Citations
4,388 Views
10 Pages

The use of non-Saccharomyces yeast species generally involves sequential or co-inoculation of a Saccharomyces cerevisiae strain to complete fermentation. While most studies have focused on characterising the impact that S. cerevisiae has on the growt...

  • Article
  • Open Access
38 Citations
7,474 Views
23 Pages

Screening for the Brewing Ability of Different Non-Saccharomyces Yeasts

  • Yvonne Methner,
  • Mathias Hutzler,
  • Dagmar Matoulková,
  • Fritz Jacob and
  • Maximilian Michel

Non-Saccharomyces yeasts have aroused interest in brewing science as an innovative and seminal way of creating new beer flavors. A screening system for potential brewing strains of non-Saccharomyces yeasts was set up to investigate the yeast’s...

  • Review
  • Open Access
63 Citations
12,627 Views
22 Pages

Non-Saccharomyces and Saccharomyces non-cerevisiae studies have increased in recent years due to an interest in uninoculated fermentations, consumer preferences, wine technology, and the effect of climate change on the chemical composition of grapes,...

  • Review
  • Open Access
56 Citations
7,704 Views
14 Pages

Drinking wine is a processed beverage that offers high nutritional and health benefits. It is produced from grape must, which undergoes fermentation by yeasts (and sometimes lactic acid bacteria) to create a product that is highly appreciated by cons...

  • Article
  • Open Access
40 Citations
7,664 Views
22 Pages

New Approaches for the Fermentation of Beer: Non-Saccharomyces Yeasts from Wine

  • Vanesa Postigo,
  • Ana Sánchez,
  • Juan Mariano Cabellos and
  • Teresa Arroyo

Non-Saccharomyces yeasts represent a very attractive alternative for the production of beers with superior sensory quality since they are able to enhance the flavour of beer. Furthermore, they can produce beers with low ethanol content due to the wea...

  • Review
  • Open Access
72 Citations
13,176 Views
13 Pages

Winemaking is a complex process involving the interaction of different microbes. The two main groups of microorganisms involved are yeasts and bacteria. Non-Saccharomyces yeasts are present on the grape surface and also on the cellar. Although these...

  • Review
  • Open Access
10 Citations
4,723 Views
34 Pages

Biocontrol and Enzymatic Activity of Non-Saccharomyces Wine Yeasts: Improvements in Winemaking

  • María Carolina Martín,
  • Luciana Paola Prendes,
  • Vilma Inés Morata and
  • María Gabriela Merín

Wine fermentation is a biochemical process carried out by a microbial consortium already present in the vineyard, including different species of fungi and bacteria that are in an ecological relationship with each other, so that their sequential growt...

  • Article
  • Open Access
5 Citations
4,172 Views
12 Pages

There a lot of studies including the use of non-Saccharomyces yeasts in the process of wine fermentation. The attention is focused on the first steps of fermentation. However, the processes and changes that the non-Saccharomyces yeast populations may...

  • Review
  • Open Access
86 Citations
11,491 Views
23 Pages

About 42 commercial products based on non-Saccharomyces yeasts are estimated as available on the market, being mostly pure cultures (79%), with a predominance of Torulaspora delbrueckii, Lachancea thermotolerans, and Metschnikowia pulcherrima. The ot...

  • Review
  • Open Access
132 Citations
8,946 Views
13 Pages

23 December 2020

Non-Saccharomyces yeast plays an important role in the initial stages of a wild ferment, as they are found in higher abundance in the vineyard than Saccharomyces cerevisiae. As such, there has been a focus in recent years to isolate these yeast speci...

  • Feature Paper
  • Article
  • Open Access
91 Citations
23,581 Views
19 Pages

Application of Non-Saccharomyces Yeasts Isolated from Kombucha in the Production of Alcohol-Free Beer

  • Konstantin Bellut,
  • Maximilian Michel,
  • Martin Zarnkow,
  • Mathias Hutzler,
  • Fritz Jacob,
  • David P. De Schutter,
  • Luk Daenen,
  • Kieran M. Lynch,
  • Emanuele Zannini and
  • Elke K. Arendt

Alcohol-free beer (AFB) is no longer just a niche product in the beer market. For brewers, this product category offers economic benefits in the form of a growing market and often a lower tax burden and enables brewers to extend their product portfol...

  • Article
  • Open Access
7 Citations
2,556 Views
10 Pages

Screening of Saccharomyces and Non-Saccharomyces Wine Yeasts for Their Decarboxylase Activity of Amino Acids

  • Gabriella Siesto,
  • Maria Rosaria Corbo,
  • Rocchina Pietrafesa,
  • Milena Sinigaglia,
  • Patrizia Romano and
  • Antonio Bevilacqua

11 November 2022

The type and quantity of precursor amino acids present in grape must that are used by wine yeasts affect the organoleptic and health properties of wine. The aim of this work was to conduct a preliminary screening among Saccharomyces and non-Saccharom...

  • Article
  • Open Access
15 Citations
5,053 Views
17 Pages

Pilot Scale Fermentations of Sangiovese: An Overview on the Impact of Saccharomyces and Non-Saccharomyces Wine Yeasts

  • Cristina Romani,
  • Livio Lencioni,
  • Alessandra Biondi Bartolini,
  • Maurizio Ciani,
  • Ilaria Mannazzu and
  • Paola Domizio

The production of wines with peculiar analytical and sensorial profiles, together with the microbiological control of the winemaking process, has always been one of the main objectives of the wine industry. In this perspective, the use of oenological...

  • Review
  • Open Access
123 Citations
12,143 Views
25 Pages

Contribution of Non-Saccharomyces Yeasts to Wine Freshness. A Review

  • Antonio Morata,
  • Carlos Escott,
  • María Antonia Bañuelos,
  • Iris Loira,
  • Juan Manuel del Fresno,
  • Carmen González and
  • José Antonio Suárez-Lepe

25 December 2019

Freshness, although it is a concept difficult to define in wines, can be understood as a combination of different circumstances. Organolepticwise, bluish red, floral and fruity, more acidic and full-bodied wines, are perceived as younger and fresher...

  • Review
  • Open Access
52 Citations
8,793 Views
18 Pages

Influence of Saccharomyces and non-Saccharomyces Yeasts in the Formation of Pyranoanthocyanins and Polymeric Pigments during Red Wine Making

  • Antonio Morata,
  • Carlos Escott,
  • Iris Loira,
  • Juan Manuel Del Fresno,
  • Carmen González and
  • Jose Antonio Suárez-Lepe

8 December 2019

Yeast are able to modulate many sensory parameters of wines during red must fermentation. The effect on color and on the formation of derived pigments during fermentation has been studied thoroughly since the 90s. Yeast can increase grape anthocyanin...

  • Article
  • Open Access
2 Citations
1,750 Views
21 Pages

Effect of Indigenous Non-Saccharomyces Yeasts on Lipid Compositions of Maraština Wine

  • Ana Boban,
  • Urska Vrhovsek,
  • Domenico Masuero,
  • Vesna Milanović and
  • Irena Budić-Leto

15 January 2025

This study is the first to investigate the impact of indigenous non-Saccharomyces yeasts, including Hypopichia pseudoburtonii, Metschnikowia sinensis/shanxiensis, Metschnikowia chrysoperlae, Metschnikowia pulcherrima, Lachancea thermotolerans, Hansen...

  • Article
  • Open Access
5 Citations
2,363 Views
15 Pages

Enhancing Flavor Complexity in Craft Beer: Sequential Inoculation with Indigenous Non-Saccharomyces and Commercial Saccharomyces Yeasts

  • María Victoria Mestre Furlani,
  • Mercedes Fabiana Vargas Perucca,
  • Diego Bernardo Petrignani,
  • Silvia Cristina Vergara,
  • María José Leiva-Alaniz,
  • Yolanda Paola Maturano,
  • Fabio Vazquez and
  • Eduardo Dellacassa

The pursuit of unique flavors in craft beer has led to the exploration of non-Saccharomyces (NS) yeasts. While Saccharomyces species dominate beer fermentation, NS yeasts offer flavor diversification. However, their lower fermentation efficiency and...

  • Article
  • Open Access
15 Citations
3,655 Views
17 Pages

Evaluation of Autochthonous Non-Saccharomyces Yeasts by Sequential Fermentation for Wine Differentiation in Galicia (NW Spain)

  • Pilar Blanco,
  • David Castrillo,
  • María José Graña,
  • María José Lorenzo and
  • Elvira Soto

Non-Saccharomyces yeasts constitute a useful tool in winemaking because they secrete hydrolytic enzymes and produce metabolites that enhance wine quality; in addition, their ability to reduce alcohol content and/or to increase acidity can help to mit...

  • Article
  • Open Access
5 Citations
4,135 Views
18 Pages

The Impact of Indigenous Non-Saccharomyces Yeasts Inoculated Fermentations on ‘Semillon’ Icewine

  • Jing Wang,
  • Yuwen Ma,
  • Faisal Eudes Sam,
  • Pingping Gao,
  • Lihong Liang,
  • Shuai Peng and
  • Min Li

The emerging low acidity in icewine grapes is becoming a major problem in producing quality icewine. Using non-Saccharomyces cerevisiae yeasts in fermentation can improve wine’s organoleptic characteristics and aromatic quality. This study eval...

  • Article
  • Open Access
15 Citations
5,729 Views
13 Pages

Biodiversity and Enological Potential of Non-Saccharomyces Yeasts from Nemean Vineyards

  • Georgios Sgouros,
  • Ioanna Chalvantzi,
  • Athanasios Mallouchos,
  • Yiannis Paraskevopoulos,
  • Georgios Banilas and
  • Aspasia Nisiotou

Vineyards in Nemea, the most important viticultural zone in Greece, were surveyed for indigenous non-Saccharomyces (NS) yeasts of enological potential. NS populations were isolated from the final stage of alcoholic fermentation and identified by a ra...

  • Article
  • Open Access
13 Citations
3,908 Views
19 Pages

Diversity of Volatile Aroma Compound Composition Produced by Non-Saccharomyces Yeasts in the Early Phase of Grape Must Fermentation

  • Doris Delač Salopek,
  • Ivana Horvat,
  • Ana Hranilović,
  • Tomislav Plavša,
  • Sanja Radeka,
  • Igor Pasković and
  • Igor Lukić

5 October 2022

There is a lack of studies evaluating the metabolic contribution of non-Saccharomyces yeasts in early fermentation phases. This study aimed to investigate the volatile aroma profiles produced by various non-Saccharomyces yeasts just before sequential...

  • Article
  • Open Access
2,623 Views
25 Pages

17 April 2025

In recent years, numerous studies have demonstrated that non-Saccharomyces yeasts hold potential for industrial application and aroma generation during fermentation. Non-Saccharomyces wild yeasts can be important tools in the development of new produ...

  • Article
  • Open Access
20 Citations
4,002 Views
18 Pages

The common use of commercial yeasts usually leads to dull wine with similar aromas and tastes. Therefore, screening for novel indigenous yeasts to practice is a promising method. In this research, aroma discrepancies among six wine groups fermentated...

  • Article
  • Open Access
37 Citations
5,966 Views
17 Pages

Optimised Extraction and Preliminary Characterisation of Mannoproteins from Non-Saccharomyces Wine Yeasts

  • Carla Snyman,
  • Julie Mekoue Nguela,
  • Nathalie Sieczkowski,
  • Matteo Marangon and
  • Benoit Divol

22 April 2021

The exogenous application of yeast-derived mannoproteins presents many opportunities for the improvement of wine technological and oenological properties. Their isolation from the cell wall of Saccharomycescerevisiae has been well studied. However, i...

  • Article
  • Open Access
14 Citations
7,793 Views
23 Pages

Native microorganisms present on grapes can influence final wine quality. Chambourcin is the most abundant hybrid grape grown in Pennsylvania and is more resistant to cold temperatures and fungal diseases compared to Vitis vinifera. Here, non-Sacchar...

  • Article
  • Open Access
7 Citations
8,219 Views
15 Pages

17 September 2021

Consumer demands for new sensory experiences have driven the research of unconventional yeasts in beer. While much research exists on the use of various common Saccharomyces cerevisiae strains as well as non-Saccharomyces yeasts, there exists a gap i...

  • Feature Paper
  • Article
  • Open Access
6 Citations
3,249 Views
10 Pages

Impact of Indigenous Non-Saccharomyces Yeasts Isolated from Madeira Island Vineyards on the Formation of Ethyl Carbamate in the Aging of Fortified Wines

  • João Micael Leça,
  • Vanda Pereira,
  • Andreia Miranda,
  • José Luis Vilchez,
  • Manuel Malfeito-Ferreira and
  • José Carlos Marques

1 May 2021

The impact of selected non-Saccharomyces yeasts on the occurrence of ethyl carbamate (EC) was evaluated. Hanseniaspora uvarum, Starmerella bacillaris, Pichia terricola, Pichia fermentans and Pichia kluyveri isolated from Madeira Island vineyards were...

  • Article
  • Open Access
41 Citations
4,331 Views
17 Pages

The Muscat Bailey A (MBA) grape, one of the most prominent grape cultivars in Korea, contains considerable amounts of monoterpene alcohols that have very low odor thresholds and significantly affect the perception of wine aroma. To develop a potentia...

  • Review
  • Open Access
24 Citations
4,644 Views
23 Pages

Biocontrol and Probiotic Function of Non-Saccharomyces Yeasts: New Insights in Agri-Food Industry

  • Francesca Comitini,
  • Laura Canonico,
  • Alice Agarbati and
  • Maurizio Ciani

Fermented food matrices, including beverages, can be defined as the result of the activity of complex microbial ecosystems where different microorganisms interact according to different biotic and abiotic factors. Certainly, in industrial production,...

  • Review
  • Open Access
38 Citations
11,349 Views
24 Pages

The interest in non-Saccharomyces yeast for use in sparkling wine production has increased in recent years. Studies have reported differences in amino acids and ammonia, volatile aroma compounds (VOCs), glycerol, organic acids, proteins and polysacch...

  • Article
  • Open Access
2 Citations
2,600 Views
17 Pages

Antimicrobial Activity of Chitosan from Different Sources Against Non-Saccharomyces Wine Yeasts as a Tool for Producing Low-Sulphite Wine

  • Francesco Tedesco,
  • Rocchina Pietrafesa,
  • Gabriella Siesto,
  • Carmen Scieuzo,
  • Rosanna Salvia,
  • Patrizia Falabella and
  • Angela Capece

28 October 2024

Chitosan is used as an antimicrobial agent in different agri-food applications; in winemaking, the use of chitosan from Aspergillus niger is authorized, but other sources of chitin, and consequently of chitosan, are available, such as crustaceans and...

  • Article
  • Open Access
59 Citations
6,809 Views
15 Pages

High citric acid content in kiwifruit wine would lead to bad sensory experience and quality deterioration. It is opportune and crucial to develop an appropriate and feasible method to degrade citric acid for kiwifruit wine. The non-Saccharomyces yeas...

  • Article
  • Open Access
331 Views
23 Pages

24 December 2025

Screening of suitable yeast strains is essential for high-quality fruit wine production. In this study, twelve Saccharomyces and non-Saccharomyces yeasts were evaluated for their performance in fermenting peach wines. Results showed that all strains...

  • Article
  • Open Access
9 Citations
3,324 Views
16 Pages

Non-Saccharomyces Yeast Strains, Aromatic Compounds and Sensory Analysis of Italy and Negra Criolla Pisco from the Moquegua Region of Peru

  • César Augusto Napa-Almeyda,
  • Celia Criado,
  • Jhony Mayta-Hancco,
  • Marcial Silva-Jaimes,
  • Luis Condezo-Hoyos and
  • María Ángeles Pozo-Bayón

In this study, we aimed to isolate and phenotypically characterize non-Saccharomyces yeast strains (NSYSs) from the skin of aromatic (Italy) and non-aromatic (Negra Criolla) grapes from vineyards in Moquegua, Peru, typically used for the production o...

  • Article
  • Open Access
11 Citations
2,504 Views
19 Pages

On the Way to the Technological Development of Newly Selected Non-Saccharomyces Yeasts Selected as Innovative Biocontrol Agents in Table Grapes

  • Antonella Salerno,
  • Margherita D’Amico,
  • Carlo Bergamini,
  • Flavia Angela Maria Maggiolini,
  • Marco Vendemia,
  • Annalisa Prencipe,
  • Claudia Rita Catacchio,
  • Mario Ventura,
  • Maria Francesca Cardone and
  • Antonio Domenico Marsico

Post-harvest decay of fresh table grapes causes considerable annual production losses. The main fungal agents of decay both in pre- and post-harvest are B. cinerea, Penicillium spp., Aspergillus spp., Alternaria spp., and Cladosporium spp. To date, t...

  • Article
  • Open Access
734 Views
18 Pages

Climate change poses a major challenge for wine production in semi-arid regions, where grape ripening frequently leads to excessive sugar accumulation and reduced acidity. This study evaluated the combined effect of cold pre-fermentative maceration (...

  • Article
  • Open Access
72 Citations
5,710 Views
16 Pages

Co-fermentation of selected non-Saccharomyces yeast strain with Saccharomyces cerevisiae is regarded as a promising approach to improve the sensory quality of fruit wine. To evaluate the effects of co-fermentations between the selected non-Saccharomy...

  • Article
  • Open Access
7 Citations
2,168 Views
16 Pages

5 March 2025

The production of strawberry wine is an effective strategy for addressing the significant economic losses caused by strawberry spoilage. In recent years, there has been an increase in consumer demand for quality and flavor diversity in fruit wines. T...

  • Article
  • Open Access
6 Citations
2,816 Views
26 Pages

Assessment of Tannin Tolerant Non-Saccharomyces Yeasts Isolated from Miang for Production of Health-Targeted Beverage Using Miang Processing Byproducts

  • Pratthana Kodchasee,
  • Nattanicha Pharin,
  • Nakarin Suwannarach,
  • Kridsada Unban,
  • Chalermpong Saenjum,
  • Apinun Kanpiengjai,
  • Dipayan Sakar,
  • Kalidas Shetty,
  • Martin Zarnkow and
  • Chartchai Khanongnuch

27 January 2023

This research demonstrated an excellent potential approach for utilizing Miang fermentation broth (MF-broth), a liquid residual byproduct from the Miang fermentation process as a health-targeted beverage. One hundred and twenty yeast strains isolated...

  • Article
  • Open Access
3 Citations
3,626 Views
12 Pages

8 September 2022

Wine quality is determined by the particular yeast strains prevailing at various stages of fermentation. Therefore, the ability to make an easy, fast, and unambiguous discrimination of yeasts at the strain level is of great importance. Here, the tand...

  • Article
  • Open Access
20 Citations
4,587 Views
26 Pages

8 July 2022

The use of non-Saccharomyces yeasts in brewing is a useful tool for developing new products to meet the growing consumer demand for innovative products. Non-Saccharomyces yeasts can be used both in single and in mixed fermentations with Saccharomyces...

  • Article
  • Open Access
2 Citations
2,887 Views
14 Pages

The Nutritional Quality of the Culture Medium Influences the Survival of Non-Saccharomyces Yeasts Co-Cultured with Saccharomyces cerevisiae

  • Erick D. Acosta-García,
  • Nicolás O. Soto-Cruz,
  • Edwin A. Valdivia-Hernández,
  • Juan A. Rojas-Contreras,
  • Martha R. Moreno-Jiménez and
  • Jesús B. Páez-Lerma

Yeast-yeast interactions have been studied mainly using wine yeasts. However, studies are rare for native yeasts from agave juice fermentation. Therefore, this work used strains isolated from the alcoholic fermentation of agave to study the survival...

  • Article
  • Open Access
97 Citations
8,781 Views
16 Pages

Effect of Sequential Inoculation with Non-Saccharomyces and Saccharomyces Yeasts on Riesling Wine Chemical Composition

  • Ophélie Dutraive,
  • Santiago Benito,
  • Stefanie Fritsch,
  • Beata Beisert,
  • Claus-Dieter Patz and
  • Doris Rauhut

In recent years, studies have reported the positive influence of non-Saccharomyces yeast on wine quality. Many grape varieties under mixed or sequential inoculation show an overall positive effect on aroma enhancement. A potential impact by non-Sacch...

  • Article
  • Open Access
58 Citations
10,419 Views
24 Pages

Effect of Saccharomyces, Non-Saccharomyces Yeasts and Malolactic Fermentation Strategies on Fermentation Kinetics and Flavor of Shiraz Wines

  • Heinrich Du Plessis,
  • Maret Du Toit,
  • Hélène Nieuwoudt,
  • Marieta Van der Rijst,
  • Martin Kidd and
  • Neil Jolly

The use of non-Saccharomyces yeasts to improve complexity and diversify wine style is increasing; however, the interactions between non-Saccharomyces yeasts and lactic acid bacteria (LAB) have not received much attention. This study investigated the...

  • Article
  • Open Access
12 Citations
2,634 Views
15 Pages

Non-Saccharomyces Yeasts from Organic Vineyards as Spontaneous Fermentation Agents

  • Lorena López-Enríquez,
  • Josefina Vila-Crespo,
  • José Manuel Rodríguez-Nogales,
  • Encarnación Fernández-Fernández and
  • Violeta Ruipérez

2 October 2023

Currently, non-Saccharomyces yeasts are the subject of interest, among other things, for their contribution to the aromatic complexity of wines. In this study, the characterisation of non-Saccharomyces yeasts was addressed by their isolation during s...

  • Review
  • Open Access
25 Citations
6,345 Views
15 Pages

Starmerella bacillaris (synonym Candida zemplinina) is a non-Saccharomyces yeast species, frequently found in enological ecosystems. Peculiar aspects of the genetics and metabolism of this yeast species, as well as potential industrial applications o...

  • Article
  • Open Access
10 Citations
2,154 Views
13 Pages

Cultivar-Dependent Effects of Non-Saccharomyces Yeast Starter on the Oenological Properties of Wines Produced from Two Autochthonous Grape Cultivars in Southern Italy

  • Vito Michele Paradiso,
  • Luigi Sanarica,
  • Ignazio Zara,
  • Chiara Pisarra,
  • Giuseppe Gambacorta,
  • Giuseppe Natrella and
  • Massimiliano Cardinale

26 October 2022

Global warming poses a threat to winemaking worldwide, especially in dry–warm regions such as Southern Italy. Must fermentation with non-Saccharomyces yeast starter is a possible approach to limit the negative effects of climate change, leading...

  • Article
  • Open Access
15 Citations
3,998 Views
20 Pages

Identification and Molecular Characterization of Novel Mycoviruses in Saccharomyces and Non-Saccharomyces Yeasts of Oenological Interest

  • Dalila Crucitti,
  • Marco Chiapello,
  • Daniele Oliva,
  • Marco Forgia,
  • Massimo Turina,
  • Francesco Carimi,
  • Francesca La Bella and
  • Davide Pacifico

29 December 2021

Wine yeasts can be natural hosts for dsRNA, ssRNA viruses and retrotransposon elements. In this study, high-throughput RNA sequencing combined with bioinformatic analyses unveiled the virome associated to 16 Saccharomyces cerevisiae and 8 non-Sacchar...

  • Article
  • Open Access
99 Citations
8,898 Views
19 Pages

Nitrogen Preferences during Alcoholic Fermentation of Different Non-Saccharomyces Yeasts of Oenological Interest

  • Helena Roca-Mesa,
  • Sonia Sendra,
  • Albert Mas,
  • Gemma Beltran and
  • María-Jesús Torija

Non-Saccharomyces yeasts have long been considered spoilage microorganisms. Currently, oenological interest in those species is increasing, mostly due to their positive contribution to wine quality. In this work, the fermentative capacity and nitroge...

of 12