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37 Results Found

  • Article
  • Open Access
28 Citations
5,249 Views
20 Pages

14 May 2019

Mycotoxins occur worldwide in the major grains, and producers, traders and processors are all challenged to prevent serious health problems for consumers. The challenges originate with pre-harvest fungi infections in the grain fields, increased conta...

  • Feature Paper
  • Article
  • Open Access
87 Citations
9,623 Views
12 Pages

Development of a LC-MS/MS Method for the Multi-Mycotoxin Determination in Composite Cereal-Based Samples

  • Barbara De Santis,
  • Francesca Debegnach,
  • Emanuela Gregori,
  • Simona Russo,
  • Francesca Marchegiani,
  • Gabriele Moracci and
  • Carlo Brera

18 May 2017

The analytical scenario for determining contaminants in the food and feed sector is constantly prompted by the progress and improvement of knowledge and expertise of researchers and by the technical innovation of the instrumentation available. Mycoto...

  • Article
  • Open Access
47 Citations
8,835 Views
14 Pages

19 July 2020

A multi-mycotoxin chromatographic method was developed and validated for the simultaneous quantitation of aflatoxins (AFB1, AFB2, AFG1 and AFG2), ochratoxin A (OTA), zearalenone (ZON), deoxynivalenol (DON), nivalenol (NIV), diacetoxyscirpenol (DAS),...

  • Article
  • Open Access
52 Citations
6,462 Views
21 Pages

15 April 2021

Rice bran, a by-product of the rice milling process, has emerged as a functional food and being used in formulation of healthy food and drinks. However, rice bran is often contaminated with numerous mycotoxins. In this study, a method to simultaneous...

  • Article
  • Open Access
16 Citations
4,266 Views
17 Pages

Biomarkers of Deoxynivalenol, Citrinin, Ochratoxin A and Zearalenone in Pigs after Exposure to Naturally Contaminated Feed Close to Guidance Values

  • Agnieszka Tkaczyk,
  • Piotr Jedziniak,
  • Łukasz Zielonka,
  • Michał Dąbrowski,
  • Piotr Ochodzki and
  • Adrianna Rudawska

22 October 2021

This study applied multi-mycotoxin liquid chromatography with tandem mass spectrometric detection (LC-MS/MS) methods to determine the biomarkers of exposure in urine and serum samples from a dose-response study with pigs. The 24 studied pigs were div...

  • Article
  • Open Access
19 Citations
3,938 Views
12 Pages

Mycotoxins are secondary metabolites produced by certain fungal species. In this study, the aim was to investigate mycotoxins, which pose a serious health problem. For this purpose, a total of 140 spice samples (black pepper, red pepper, cumin, and t...

  • Article
  • Open Access
9 Citations
2,692 Views
12 Pages

2 July 2022

A high-performance liquid chromatography tandem mass spectrometry method is described for the simultaneous determination of mycotoxins, including Ergot alkaloids (EAs), in 3 types of grains. The extraction of 23 mycotoxins was evaluated and performed...

  • Review
  • Open Access
23 Citations
9,214 Views
30 Pages

23 August 2021

Farm animals are frequently exposed to mycotoxins, which have many adverse effects on their health and become a significant food safety issue. Pigs are highly exposed and particularly susceptible to mycotoxins, which can cause many adverse effects. F...

  • Article
  • Open Access
67 Citations
9,467 Views
30 Pages

19 March 2019

A reliable and practical multi-method was developed for the quantification of mycotoxins in plasma, urine, and feces of pigs, and plasma and excreta of broiler chickens using liquid chromatography–tandem mass spectrometry. The targeted mycotoxi...

  • Article
  • Open Access
97 Citations
11,994 Views
17 Pages

Rapid Detection and Identification of Mycotoxigenic Fungi and Mycotoxins in Stored Wheat Grain

  • Sudharsan Sadhasivam,
  • Malka Britzi,
  • Varda Zakin,
  • Moshe Kostyukovsky,
  • Anatoly Trostanetsky,
  • Elazar Quinn and
  • Edward Sionov

25 September 2017

This study aimed to assess the occurrence of toxigenic fungi and mycotoxin contamination in stored wheat grains by using advanced molecular and analytical techniques. A multiplex polymerase chain reaction (PCR) strategy was established for rapid iden...

  • Article
  • Open Access
58 Citations
5,120 Views
21 Pages

Mycotoxins in Flanders’ Fields: Occurrence and Correlations with Fusarium Species in Whole-Plant Harvested Maize

  • Jonas Vandicke,
  • Katrien De Visschere,
  • Siska Croubels,
  • Sarah De Saeger,
  • Kris Audenaert and
  • Geert Haesaert

Mycotoxins are well-known contaminants of several food- and feedstuffs, including silage maize for dairy cattle. Climate change and year-to-year variations in climatic conditions may cause a shift in the fungal populations infecting maize, and theref...

  • Article
  • Open Access
16 Citations
6,080 Views
11 Pages

21 March 2022

To monitor co-exposure to toxic mycotoxins in dried fruits, it is advantageous to simultaneously determine multiple mycotoxins using a single extraction and liquid chromatography with tandem mass spectrometry (LC-MS/MS) analysis. In this study, we ap...

  • Article
  • Open Access
15 Citations
4,911 Views
10 Pages

7 December 2021

In this study, a total of 85 cereal-based baby foods with or without milk (four different brands; A, B, C, and D) collected from Ankara local markets, Turkey were analyzed for mycotoxins, total aerobic mesophilic bacteria (TAMB), and Enterobacteriace...

  • Article
  • Open Access
26 Citations
3,925 Views
13 Pages

28 April 2022

The use of plant-based fish feed may increase the risk of contamination by mycotoxins. The multiresidue analysis of mycotoxins in fish feed presents many difficulties due to the complexity of the matrix, the different characteristics of the compounds...

  • Article
  • Open Access
7 Citations
2,582 Views
13 Pages

30 June 2022

The European Food Safety Authority is currently evaluating the risks related to the presence of emerging mycotoxins in food and feeds. The aim of this study was to investigate the role of soil fertility, resulting from different nitrogen fertilizatio...

  • Article
  • Open Access
9 Citations
2,456 Views
12 Pages

Algerian Workers’ Exposure to Mycotoxins—A Biomonitoring Study

  • Marta I. Mendes,
  • Sara C. Cunha,
  • Iméne Rebai and
  • José O. Fernandes

Mycotoxins, produced by fungi as secondary metabolites, have the potential to induce both short-term and long-term toxic consequences in animals and humans. The present study aimed to determine multi-mycotoxin levels in Algerian workers using urine a...

  • Article
  • Open Access
27 Citations
4,509 Views
12 Pages

26 January 2022

In this study, a total of 80 peanut butter, hazelnut butter, and chocolate samples were obtained from local markets in Ankara, Turkey. These foods were analyzed for twelve toxicological important mycotoxins, such as aflatoxin B1 (AFB1), aflatoxin B2...

  • Article
  • Open Access
86 Citations
10,265 Views
18 Pages

Occurrence of Fusarium Mycotoxins in Cereal Crops and Processed Products (Ogi) from Nigeria

  • Cynthia Adaku Chilaka,
  • Marthe De Boevre,
  • Olusegun Oladimeji Atanda and
  • Sarah De Saeger

18 November 2016

In Nigeria, maize, sorghum, and millet are very important cash crops. They are consumed on a daily basis in different processed forms in diverse cultural backgrounds. These crops are prone to fungi infestation, and subsequently may be contaminated wi...

  • Article
  • Open Access
49 Citations
5,073 Views
19 Pages

Variation of Fusarium Free, Masked, and Emerging Mycotoxin Metabolites in Maize from Agriculture Regions of South Africa

  • Theodora Ijeoma Ekwomadu,
  • Toluwase Adeseye Dada,
  • Nancy Nleya,
  • Ramokone Gopane,
  • Michael Sulyok and
  • Mulunda Mwanza

28 February 2020

The presence of mycotoxins in cereal grain is a very important food safety issue with the occurrence of masked mycotoxins extensively investigated in recent years. This study investigated the variation of different Fusarium metabolites (including the...

  • Article
  • Open Access
25 Citations
5,395 Views
12 Pages

Determination of Regulated and Emerging Mycotoxins in Organic and Conventional Gluten-Free Flours by LC-MS/MS

  • Zoe Giannioti,
  • Beatriz Albero,
  • María Dolores Hernando,
  • Luana Bontempo and
  • Rosa Ana Pérez

14 February 2023

Gluten-free cereal products have grown in popularity in recent years as they are perceived as “healthier” alternatives and can be safely consumed by celiac patients, and people with gluten intolerance or wheat allergies. Molds that produc...

  • Article
  • Open Access
46 Citations
6,459 Views
22 Pages

Multi-Mycotoxin Contamination of Maize Silages in Flanders, Belgium: Monitoring Mycotoxin Levels from Seed to Feed

  • Jonas Vandicke,
  • Katrien De Visschere,
  • Maarten Ameye,
  • Siska Croubels,
  • Sarah De Saeger,
  • Kris Audenaert and
  • Geert Haesaert

11 March 2021

Maize silage, which in Europe is the main feed for dairy cattle in winter, can be contaminated by mycotoxins. Mycotoxigenic Fusarium spp. originating from field infections may survive in badly sealed silages or re-infect at the cutting edge during fe...

  • Article
  • Open Access
18 Citations
3,788 Views
16 Pages

Fullerol C60(OH)24 Nanoparticles and Drought Impact on Wheat (Triticum aestivum L.) during Growth and Infection with Aspergillus flavus

  • Tihomir Kovač,
  • Tihana Marček,
  • Bojan Šarkanj,
  • Ivana Borišev,
  • Maja Ižaković,
  • Katarina Jukić,
  • Ante Lončarić,
  • Tamara Krska,
  • Michael Sulyok and
  • Rudolf Krska

22 March 2021

Fullerol C60(OH)24 nanoparticles (FNP)-wheat-A. flavus interaction outcome is more complicated in the presence of drought. This study sheds light on how the presence of FNP affects food and feed safety from the perspective of mycotoxin contamination....

  • Review
  • Open Access
33 Citations
9,233 Views
37 Pages

5 June 2021

The first part of this study evaluates the occurrence of mycotoxin patterns in feedstuffs and fish feeds. Results were extrapolated from a large data pool derived from wheat (n = 857), corn (n = 725), soybean meal (n = 139) and fish feed (n = 44) sam...

  • Article
  • Open Access
53 Citations
8,997 Views
16 Pages

Low Doses of Mycotoxin Mixtures below EU Regulatory Limits Can Negatively Affect the Performance of Broiler Chickens: A Longitudinal Study

  • Oluwatobi Kolawole,
  • Abigail Graham,
  • Caroline Donaldson,
  • Bronagh Owens,
  • Wilfred A. Abia,
  • Julie Meneely,
  • Michael J. Alcorn,
  • Lisa Connolly and
  • Christopher T. Elliott

1 July 2020

Several studies have reported a wide range of severe health effects as well as clinical signs, when livestock animals are exposed to high concentration of mycotoxins. However, little is known regarding health effects of mycotoxins at low levels. Thus...

  • Article
  • Open Access
31 Citations
4,858 Views
14 Pages

Multi-Occurrence of Twenty Mycotoxinsin Pasta and a Risk Assessment in the Moroccan Population

  • Youssef Bouafifssa,
  • Lara Manyes,
  • Mohamed Rahouti,
  • Jordi Mañes,
  • Houda Berrada,
  • Abdellah Zinedine and
  • Mónica Fernández-Franzón

26 October 2018

In the present study, the multi-occurrence of twenty (20) mycotoxins in pasta samples consumed in Morocco was assessed. For this, a modified Quick, Easy, Cheap Effective, Rugged, and Safe method was validated. The mycotoxins studied were identified a...

  • Article
  • Open Access
51 Citations
5,103 Views
15 Pages

Multiple Fungal Metabolites Including Mycotoxins in Naturally Infected and Fusarium-Inoculated Wheat Samples

  • Valentina Spanic,
  • Zorana Katanic,
  • Michael Sulyok,
  • Rudolf Krska,
  • Katalin Puskas,
  • Gyula Vida,
  • Georg Drezner and
  • Bojan Šarkanj

In this study, the occurrence of multiple fungal metabolites including mycotoxins was determined in four different winter wheat varieties in a field experiment in Croatia. One group was naturally infected, while the second group was inoculated with a...

  • Article
  • Open Access
2 Citations
1,995 Views
15 Pages

7 March 2025

This study investigated the impact of baking conditions on mycotoxin content in Greek pita bread, a single-layered flatbread baked at high temperatures for short time intervals. Dough samples were artificially contaminated with a multi-mycotoxin mixt...

  • Article
  • Open Access
4 Citations
5,539 Views
23 Pages

An Interlaboratory Comparison Study of Regulated and Emerging Mycotoxins Using Liquid Chromatography Mass Spectrometry: Challenges and Future Directions of Routine Multi-Mycotoxin Analysis including Emerging Mycotoxins

  • David Steiner,
  • Armin Humpel,
  • Eleonore Stamminger,
  • Anna Schoeberl,
  • Gerlinde Pachschwoell,
  • Anita Sloboda,
  • Christy Swoboda,
  • Jolene Rigg,
  • Dawei Zhang and
  • Alexandra Malachová
  • + 16 authors

13 June 2022

The present interlaboratory comparison study involved nine laboratories located throughout the world that tested for 24 regulated and non-regulated mycotoxins by applying their in-house LC-MS/MS multi-toxin method to 10 individual lots of 4 matrix co...

  • Article
  • Open Access
15 Citations
4,747 Views
16 Pages

Fullerol C60(OH)24 Nanoparticles Affect Secondary Metabolite Profile of Important Foodborne Mycotoxigenic Fungi In Vitro

  • Tihomir Kovač,
  • Bojan Šarkanj,
  • Ivana Borišev,
  • Aleksandar Djordjevic,
  • Danica Jović,
  • Ante Lončarić,
  • Jurislav Babić,
  • Antun Jozinović,
  • Tamara Krska and
  • Rudolf Krska
  • + 3 authors

27 March 2020

Despite the efforts to control mycotoxin contamination worldwide, extensive contamination has been reported to occur in food and feed. The contamination is even more intense due to climate changes and different stressors. This study examined the impa...

  • Article
  • Open Access
6 Citations
3,582 Views
12 Pages

Assessment of Human Mycotoxin Exposure in Hungary by Urinary Biomarker Determination and the Uncertainties of the Exposure Calculation: A Case Study

  • Judit Szabó-Fodor,
  • Mária Szeitzné-Szabó,
  • Brigitta Bóta,
  • Tamás Schieszl,
  • Cserne Angeli,
  • Lucia Gambacorta,
  • Michele Solfrizzo,
  • András Szabó and
  • Melinda Kovács

22 December 2021

Urinary biomarkers of mycotoxin exposure were evaluated in the case of healthy people (n = 41) and coeliac patients (n = 19) by using a multi-biomarker LC-MS/MS immunoaffinity based method capable to analyse biomarkers of nine mycotoxins, i.e., fumon...

  • Article
  • Open Access
36 Citations
7,814 Views
18 Pages

Deoxynivalenol & Deoxynivalenol-3-Glucoside Mitigation through Bakery Production Strategies: Effective Experimental Design within Industrial Rusk-Making Technology

  • Silvia Generotti,
  • Martina Cirlini,
  • Alexandra Malachova,
  • Michael Sulyok,
  • Franz Berthiller,
  • Chiara Dall'Asta and
  • Michele Suman

24 July 2015

In the scientific field, there is a progressive awareness about the potential implications of food processing on mycotoxins especially concerning thermal treatments. High temperatures may cause, in fact, transformation or degradation of these compoun...

  • Article
  • Open Access
125 Citations
10,582 Views
16 Pages

Faces of a Changing Climate: Semi-Quantitative Multi-Mycotoxin Analysis of Grain Grown in Exceptional Climatic Conditions in Norway

  • Silvio Uhlig,
  • Gunnar Sundstøl Eriksen,
  • Ingerd Skow Hofgaard,
  • Rudolf Krska,
  • Eduardo Beltrán and
  • Michael Sulyok

27 September 2013

Recent climatological research predicts a significantly wetter climate in Southern Norway as a result of global warming. Thus, the country has already experienced unusually wet summer seasons in the last three years (2010–2012). The aim of this pilot...

  • Article
  • Open Access
17 Citations
5,210 Views
32 Pages

Cocktails of Mycotoxins, Phytoestrogens, and Other Secondary Metabolites in Diets of Dairy Cows in Austria: Inferences from Diet Composition and Geo-Climatic Factors

  • Felipe Penagos-Tabares,
  • Ratchaneewan Khiaosa-ard,
  • Marlene Schmidt,
  • Eva-Maria Bartl,
  • Johanna Kehrer,
  • Veronika Nagl,
  • Johannes Faas,
  • Michael Sulyok,
  • Rudolf Krska and
  • Qendrim Zebeli

15 July 2022

Dairy production is a pivotal economic sector of Austrian and European agriculture. Dietary toxins and endocrine disruptors of natural origin such as mycotoxins and phytoestrogens can affect animal health, reproduction, and productivity. This study c...

  • Article
  • Open Access
15 Citations
4,461 Views
26 Pages

Mixtures of Mycotoxins, Phytoestrogens, and Other Secondary Metabolites in Whole-Plant Corn Silages and Total Mixed Rations of Dairy Farms in Central and Northern Mexico

  • Felipe Penagos-Tabares,
  • Michael Sulyok,
  • Juan-Ignacio Artavia,
  • Samanta-Irais Flores-Quiroz,
  • César Garzón-Pérez,
  • Ezequías Castillo-Lopez,
  • Luis Zavala,
  • Juan-David Orozco,
  • Johannes Faas and
  • Qendrim Zebeli
  • + 1 author

13 February 2023

Mycotoxins and endocrine disruptors such as phytoestrogens can affect cattle health, reproduction, and productivity. Most studies of mycotoxins in dairy feeds in Mexico and worldwide have been focused on a few (regulated) mycotoxins. In contrast, les...

  • Article
  • Open Access
33 Citations
6,376 Views
11 Pages

30 November 2017

Palm kernel cake (PKC) is the solid residue following oil extraction of palm kernels and useful to fatten animals either as a single feed with only minerals and vitamins supplementation, or mixed with other feedstuffs such as corn kernels or soy bean...

  • Article
  • Open Access
25 Citations
6,440 Views
21 Pages

13 April 2019

New protein sources in fish feed require the assessment of the carry-over potential of contaminants and anti-nutrients from feed ingredients into the fish, and the assessment of possible health risks for consumers. Presently, plant materials includin...

  • Article
  • Open Access
264 Views
15 Pages

Matrix Effect Variability in Urine Samples from Different Cohorts and Implications on LC-ESI-MS Mycotoxin Biomarker Analysis

  • Michael Kuhn,
  • Åsa Svanström,
  • Nicholas N. A. Kyei,
  • Sanna Lignell,
  • Hans-Ulrich Humpf and
  • Benedikt Cramer

10 March 2026

Matrix effects (ME) during LC-ESI-MS analysis are a commonly acknowledged issue for a variety of matrices and analytes. Although sample preparation techniques are steadily evolving to reduce ME, the complexity and variability of the urine matrix rema...