Search Results (761)

In recent years, the applications of melatonin (MT) and hydrogen sulfide (H₂S) have both been proven to improve the post-harvest preservation of horticultural products. However, the specific regulatory mechanism between them in cut flower preservation remains unclear. Here, we conducted research on the combined effect of MT and H₂S during the senescence of cut roses ‘Corolla’, as well as the relationship between endogenous H₂S production and MT. We found that the cut roses treated with MT + H₂S exhibited the longest vase life, the largest flower diameter, and the highest increase of fresh weight compared with those under MT or H₂S treatment alone. We have found that both MT and H₂S can inhibit the contents of the ethylene precursor ACC and the key enzymes ACS and ACO involved in ethylene synthesis in cut roses. Further investigation revealed that the application of hypotaurine (HT), a specific chemical scavenger of hydrogen sulfide (H₂S), compromised the preservation of cut roses. In the combined treatment with HT and melatonin (MT), elevated levels of reactive oxygen species (ROS), along with reduced antioxidant enzyme activities and downregulated expression of associated genes, were observed. However, the combined treatment still demonstrated a superior preservative effect relative to the HT treatment alone. Conversely, MT treatment individually enhanced endogenous H₂S production and upregulated the transcript levels of the H₂S biosynthesis genes RhLCD and RhDCD in petal tissues. These results substantiate that MT extends the postharvest longevity of cut roses by stimulating H₂S synthesis, thus counteracting oxidative damage. Full article

Preserving minimally processed fruits represents a technological challenge. Therefore, non-thermal plasma (NTP) is proposed as one of the preservation methods. The aim of this work was to evaluate the effect of the application of NTP before and after packaging sliced apples in two films: One with high and another with low oxygen barrier features. Different parameters were evaluated during 14 days at 4 °C. Samples treated before packaging showed lower crunchiness and browning development, as indicated by chromatic and textural parameters. NTP reduced mesophiles, psychrotrophs, and yeasts and molds on the slices by 1–2 log units, although it had no effect on the antioxidant content of the apple slices, which were better preserved with high-barrier packaging. Samples treated with NTP, and low barrier packaging showed lower sugar content on day one. When applied after packaging, NTP contributed to better preservation of slice microstructure and tissue viability. Results showed that the combination of NTP applied after packaging and high-barrier film was the most suitable for maintaining fruit quality, mainly by better preserving slice color. In turn, tissue microstructure, texture analysis, and viability tests also supported this conclusion. Full article

Blockchain-assisted off-chain storage for IoT must simultaneously manage low-latency tiered data placement, trusted and dynamic on-chain mapping, migration consistency, and failure recovery—four concerns that existing designs address in isolation. Tiered storage systems optimize placement without modeling the scalable coordination cost of keeping object–location bindings trustworthy, while blockchain-metadata studies assume static storage topologies with no dynamic tier migration. This paper presents a scalable and trusted metadata-coordinated tiered off-chain storage framework, which bridges traditional trust systems (e.g., legacy authentication) with blockchain networks powered by Proof of Capacity (PoC) consensus. In this framework, adaptive heat-driven placement, dynamic on-chain mapping evolution with batched commitment, migration-aware redirect control, and rollback-safe recovery operate as a single coordinated workflow, with the five-stage write–verify–commit–redirect–retire pipeline acting as a lightweight coordination protocol that maintains ordered and atomic state transitions under message loss, out-of-order delivery, and single-node failures. The distinctive contribution lies in the framework’s coupled control: every placement decision propagates through a verifiable metadata path that can be audited and, when necessary, rolled back. Simulation across multiple workload patterns shows that the proposed method reduces average access latency by 28% and raises the hot-tier hit ratio from 0.19 to 0.65 relative to a dynamic baseline without trusted mapping coordination under the simulated registry write cost. To achieve high-throughput mapping operations, batched on-chain commitment cuts metadata transactions by 50× at the cost of a tunable mapping freshness delay. The framework scales from 1 k to 50 k managed objects, effectively managing tens of millions of bytes of data (10+ MB scale) without disproportionate overhead growth; beyond this scale, hot-tier capacity rather than coordination becomes the dominant bottleneck, and smarter predictive placement becomes the natural next lever. All tested fault types achieve 100% rollback success with sub-millisecond local data plane interruption; audit-visible recovery depends on the assumed chain finality delay and, for heavily regulated IoT domains, such as finance and healthcare, should be treated as the operationally binding recovery time objective. These results, together with extended evaluations—including asymmetric write latency stress, coordination ablation, tail latency analysis, and benefit–complexity assessment—provide quantitative evidence that scalable, dynamic mapping coordination can be integrated into tiered off-chain data management at an acceptable and measurable operational cost under the simulated configuration. Full article

This paper investigates the valorization of peanut shell powder (PSP), an abundant agro-industrial residue, as a biofiller for the development of sustainable 3D printable PLA-based composites for food packaging applications. A low-filled biocomposite containing 2.5 wt.% PSP was successfully processed into filament with dimensional tolerances suitable for fused deposition modeling printing. Thermal and melt flow analyses demonstrated that PSP marginally reduced the thermal stability of PLA while preserving its thermal transition temperatures and increasing the melt flow rate up to 51%. Differential scanning calorimetry revealed a slight increase in crystallinity in biocomposite filament compared to neat PLA pellets, mainly associated with thermo-mechanical processing of the extrusion, while the lower crystallinity degree relative to PLA extrudate suggested a negligible nucleating effect of PSP. To optimize print quality, different extrusion temperatures and infill flow rates were evaluated. The best mechanical performance was achieved at 200 °C and 130% flow rate, where reduced inter-filament porosity (5.2%) resulted in improved tensile strength and stiffness compared with the other printing conditions. Although mechanical properties remained lower than neat PLA, the material proved suitable for non-structural packaging applications. Prototype packaging boxes were fabricated and tested for the storage of fresh-cut melon. Compared with neat PLA packaging, the PLA-PSP system better preserved fruit firmness over 10 days, inhibited fungal growth, and delayed visible deterioration, highlighting the potential active role of PSP in food preservation. Anaerobic biodegradation tests conducted under mesophilic conditions confirmed that the addition of PSP did not hinder PLA biodegradability and slightly enhanced methane production. Overall, the results demonstrate that peanut shell waste can be effectively upcycled into functional 3D-printable biocomposites for sustainable packaging solutions. Full article

Fruits and vegetables are vulnerable to enhanced respiratory metabolism, water loss, enzymatic browning, and microbial invasion during postharvest storage and transportation, leading to spoilage and reduced shelf life. Traditional preservation methods, such as physical, chemical, and single-component film technologies, offer limited benefits due to their single function and instability. Composite films, however, show great promise in postharvest preservation by integrating the strengths of different substrates and active components. This review discusses the research progress of composite films in preserving fruits and vegetables, covering their definition, classification, and key performance characteristics. It also examines the functional roles of substrates like polysaccharides, proteins, and lipids, and explores the mechanisms of composite films, including gas regulation, metabolic inhibition, water retention, antimicrobial effects, antioxidant activity, and browning delay. Additionally, it compares the applications of composite films in whole and fresh-cut fruits and vegetables. Current research highlights the shift from general applications to targeted designs based on the unique deterioration mechanisms of different produce. Future studies should focus on optimizing the matching between product characteristics and film design, advancing composite films towards safer, more stable, and practical applications. Full article

Niacin contents vary significantly among fresh-cut potato cultivars with different browning sensitivities, whereas its role as a browning inhibitor for fresh-cut produce has not been previously reported. In this study, potato slices were soaked in distilled water (control) or 1% food-grade niacin solution for 5 min, then stored at 4 ± 1 °C for 8 days with sampling every 2 days for physiological and molecular analyses. In particular, the optimal niacin (1%) treatment showed higher brightness and lower color change than the control. The activities of polyphenol oxidase (PPO), peroxidase (POD), and phenylalanine ammonia lyase (PAL), and phenol content were reduced. Higher activities of superoxide dismutase (SOD) and catalase (CAT), and greater glutathione accumulation, were observed following niacin treatment. Meanwhile, lower levels of malondialdehyde and reactive oxygen species (ROS), and lower nicotinamide adenine dinucleotide phosphate oxidase (NOX) activity, indicated lower oxidant damage. The contents of NADP and NAD, and activities of nicotinamide adenine dinucleotide kinase (NADK) and glucose-6-phosphate dehydrogenase (G6PDH) were improved. Furthermore, the gene expression patterns of StRBOH, StPPO, and StG6PDH also supported the hypothesis that niacin regulates pyridine nucleotide and ROS homeostasis. Full article

Background: Plant essential oils are extensively utilized for their antimicrobial properties; however, the specific antifungal mechanisms of certain compounds are not well characterized. Geraniol, a naturally occurring monoterpene alcohol approved for use in foods, demonstrates potential efficacy against spoilage fungi, yet detailed mechanistic insights are lacking. Methods: In this study, we determined the minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) of geraniol against P. polonicum. We assessed the underlying mechanisms by evaluating membrane integrity, intracellular leakage, reactive oxygen species (ROS), antioxidant enzymes (superoxide dismutase [SOD], peroxidase [POD], catalase [CAT]), malondialdehyde (MDA) levels, ATP content, and ATPase activity. Inoculated yam slices were exposed to geraniol vapor, and we monitored sensory, physicochemical, enzymatic, and microbial parameters. Results: Geraniol exhibited a minimum inhibitory concentration/minimum fungicidal concentration (MIC/MFC) of 0.3 mL/L. It disrupted cellular membranes, induced leakage, generated ROS, and caused lipid peroxidation, leading to elevated levels of malondialdehyde (MDA). Additionally, geraniol activated antioxidant enzymes and impaired energy metabolism. Fumigation with geraniol dose-dependently delayed the deterioration of yam, reduced weight loss, preserved texture and color, inhibited polyphenol oxidase (PPO) and POD activities, enhanced CAT and SOD activities, lowered MDA levels, and suppressed bacterial growth. Conclusions: Geraniol inhibits P. polonicum through multiple mechanisms, including membrane disruption, oxidative stress, and interference with energy metabolism, thereby effectively preserving the quality of fresh-cut yam and demonstrating potential as a natural preservative. Full article

This paper presents a comprehensive review on near-infrared (NIR) spectroscopy applied in food analysis, systematically elaborating its core principles, widespread industrial applications, advanced chemometric strategies, and cutting-edge technological progress. NIR spectroscopy (760–2500 nm), characterized by rapid, non-destructive detection and minimal sample preparation, has been widely implemented in quality evaluation and safety monitoring of grains, meat, fruits and vegetables, dairy, fermented products, tea, coffee, and other processed foods, realizing quantitative analysis of nutrients, freshness assessment, texture prediction, adulteration identification, origin tracing, and rapid preliminary screening of toxin/pesticide residues. A series of chemometric methods, including spectral preprocessing (SNV, MSC, S-G smoothing), feature extraction, and variable selection (CARS, PSO-CMW, ICPA), as well as linear/nonlinear modeling algorithms (PLS, SVM, BP-ANN, fuzzy clustering) significantly boost the accuracy and robustness of spectral analysis. Meanwhile, portable NIR devices and online monitoring systems promote on-site and real-time detection in food supply chains. Despite existing challenges such as calibration transfer, matrix interference, and model generalization, innovations like multimodal data fusion, deep learning integration, and intelligent algorithm optimization offer effective solutions. This review not only summarizes the latest research advances of NIR technology in the food field but also emphasizes its significant advantages as a rapid, non-destructive complementary tool to traditional destructive detection methods, providing theoretical support and technical reference for accelerating the industrial translation and standardized application of NIR spectroscopy, and ultimately safeguarding global food quality and safety. Full article

This study evaluated a post-cut treatment combining sanitization, carboxymethylcellulose (CMC) coating, and chokeberry pomace extract for preserving fresh-cut sweet peppers during 7 days of refrigerated storage. Sliced peppers of two cultivars, Sunny F₁ (yellow) and Yecla F₁ (red), were assigned to five treatments: water washing (control), BioActiW 2000 Food sanitizer (BAW), BAW followed by CMC coating (BAW + CMC), CMC coating with 3.5% chokeberry extract (CMC + AE), and 3.5% aqueous chokeberry extract (AAE). Samples were stored at 5 ± 1 °C and assessed for physicochemical, microbiological, sensory, and postharvest quality attributes. The response was cultivar-dependent. Coating-based treatments reduced polyphenol and L-ascorbic acid contents, although chokeberry-containing formulations mitigated these losses relative to BAW + CMC. Total sugars and carotenoids were not significantly affected. In both cultivars, BAW and BAW + CMC best limited mesophilic bacteria and yeast growth, reduced softening, and decreased weight loss. AAE applied without prior sanitization increased microbial counts in Sunny F₁. Sensory analysis showed cultivar-specific acceptance: Sunny F₁ tolerated CMC + AE and BAW + CMC better, whereas Yecla F₁ was more sensitive to off-flavors linked to the extract. These results indicate that sanitization is essential for microbiological stability, while CMC can provide an additional barrier effect. Chokeberry pomace extract showed mixed effects and appears to be a formulation component whose usefulness depends on cultivar and treatment conditions. Full article

The fresh-cut avocado processing generates significant amounts of by-products, mainly peel and seed, with the peel representing a valuable source of phenolic compounds. In this context, the growing demand for sustainable technologies encourages the use of green solvents for bioactive compound recovery. In this study, natural deep eutectic solvents (NaDES) were evaluated as environmentally friendly solvents for the extraction of phenolic compounds from Hass avocado peels through ultrasound-assisted extraction and for their potential application in fresh-cut avocado. Phenolics were extracted using acidic water, ethanol, and NaDES based on choline chloride as a fixed hydrogen bond acceptor (HBA) and hydrogen bond donors (HBDs; lactic acid, glycerol, and citric acid) with the ultrasound-assisted system. The stability of the extracts was monitored for eight weeks (four weeks in darkness followed by four weeks under light exposure). Among the tested formulations, the lactic-acid-based NaDES showed the highest extraction efficiency and the best stability of phenolic compounds during storage (≥20 mg GAE g⁻¹ dw during the storage period). The lactic-acid-based extract was then applied to fresh-cut avocado to evaluate its potential for antioxidant enrichment and browning prevention during refrigerated storage. The treatment increased phenolic content and contributed to improved color stability (during seven days of storage). Overall, lactic-acid-based NaDES represent a promising green solvent system for recovering phenolics from avocado peel and for their functional application in fresh-cut avocado within a circular valorization approach. Full article

Rotary-percussive drilling is extensively used for efficient hard rock breakage, and the performance of impregnated diamond bits (IDBs) is primarily governed by matrix characteristics and diamond parameters. However, under impact conditions, diamonds do not behave as static cutting elements. Instead, they undergo a continuous cycle of microfracture (creating fresh sharp edges), intact retention (maintaining stability), and matrix wear-induced exposure (renewal). This work reveals this impact-driven dynamic balance mechanism. Fe-based matrix IDBs with different carbon fiber contents (regulating matrix hardness) and diamond parameters (concentration, particle size) were fabricated to study the effects of relevant parameters on bit wear and drilling performance under rotary-percussive drilling conditions. Within the experimental scope, it was found that carbon fiber can reduce the torque during drilling. The optimal balance of the three phases occurs at a matrix hardness of 95.8 HRB, where the combined proportion of micro-fracture and whole diamonds reaches 69.9% and emerging diamonds 12.9%, yielding the highest wear performance index α = 0.236. With increasing diamond concentration, the rate of penetration (ROP) and diamond exposure height decreased and the proportion of blunt diamond increased; the best balance is at an 80% concentration (α = 0.213). When the diamond mesh size increases, the ROP decreases rapidly, the torque first decreases and then increases, the proportion of whole diamonds first increases and then decreases, and the proportion of pull-out diamonds first decreases and then increases. The optimal mesh size is #50/60 (α = 0.241). This study not only provides parameter optimization, but also offers a mechanical understanding of how impact controls diamond self-sharpening and renewal, providing a new foundation for designing IDBs for impact rotary drilling. Full article

Efficient propagation of Hydrangea arborescens is essential for the stable production of high-quality plantlets. However, propagation via stem cuttings is often limited by environmental conditions and inconsistent rooting. This study aimed to identify an effective in vitro culture medium by integrating quantitative growth traits with image-based quality analysis. Seven culture media (M1–M7), consisting of Murashige and Skoog (MS), McCown Woody Plant (McCown), and Gamborg B5 basal media supplemented with different plant growth regulator combinations, were evaluated based on shoot number, root number, plant height, and fresh weights, and plantlet quality was assessed using Green area, ExG (excess green index), and a composite z-score. Significant differences were observed among treatments. M5 and M7 produced the highest shoot numbers, and M7 showed the greatest fresh weight. Image-based analysis indicated that M2 and M7 exhibited the highest overall quality, whereas M3 showed the lowest performance. Basal media types did not significantly affect plantlet quality, whereas hormone treatments enhanced both shoot multiplication and callus formation. A positive association was observed between callus formation rate and shoot number (Spearman’s ρ = 0.74, p < 0.001). Overall, M7 (Gamborg B5 medium supplemented with 30 g∙L⁻¹ sucrose, 1.5 mg∙L⁻¹ BA, and 0.25 g∙L⁻¹ gelrite) provided a balanced combination of high propagation efficiency and plantlet quality, and these findings contribute to the efficient production of high-quality planting materials. Full article

UV-curable L(-)–borneol-functionalized antibacterial hydrogels for packaging fresh-cut banana and cherry tomato (UV-LBs) were designed from L(-)–borneol-functionalized polyurethane acrylate prepolymers (LB-PUAs) and thiol-functionalized PVA (PVA-SH) using a thiol-ene click reaction initiated by UV light. UV-LBs exhibit unique properties, including excellent thermal stability, high mechanical performance and quite high antibacterial efficiency. The initial thermal decomposition temperature (T_d5), tensile strength and elongation at break are in the range of 225–240 °C, 1.38–2.05 MPa and 44.4–68.6%, respectively. The antibacterial efficiency of UV-LBs against Staphylococcus aureus (S. aureus), Escherichia coli (E. coli), and Monilia albican (M. albican) can reach 67.4%, 75.6% and 83.7%, respectively. The storage time of packaged fresh-cut banana and cherry tomato can be extended from 12 h to 30 h and 4 d to 5 d, respectively. Full article

Cermet tools possess favorable mechanical and tribological properties and are widely adopted for machining hard-to-cut materials. However, their performance can further be enhanced with different cooling and lubrication techniques. In this study, the tool wear mechanisms of cermet tools during hard turning of AISI 4340 alloy steel are investigated under dry and minimum quantity lubrication (MQL) environments to identify the prevalent causes of tool failure through comprehensive analysis of tool wear progression, chip temperature, and chip morphological analysis. The results revealed that the application of MQL exhibited prolonged and stable steady-state tool wear progression with retained cutting-edge geometry, thus demonstrated 30.27% improvement in tool life compared to dry cutting. On the contrary, a rapid increase in tool wear due to excessive friction and higher thermal load is noticed with dry cutting in the absence of any heat-dissipating medium. Chip temperature measurements supported these observations, as chip temperature increases sharply from 358 °C (with a fresh tool) to about 1090 °C (with a worn tool) under a dry environment. Conversely, with MQL, the corresponding increase was in the range between 294 °C and 843 °C with a fresh and worn tool, respectively. Chip analysis revealed a serrated type of chip morphology. Dry cutting exhibited intensified feed marks, indicative of severe tool–chip friction, whereas MQL demonstrated smoother morphology with closely spaced saw-tooth patterns. Tool wear mechanisms indicate abrasion, adhesion, and edge chipping as dominant wear mechanisms under both environments; however, in the absence of any lubricant, these mechanisms were more intensified with higher crater formation. Full article

Canola meal, with environmentally friendly attributes, lower cost, and previous studies, is an interesting proteic source to partially replace the soybean meal for poultry nutrition. For that, in this work we aimed to investigate canola meal as a partial replacement of soybean meal in finishing poultry diets (21 to 49 days) on the productive performance, also including the impact on the quality, nutritional attributes and antioxidative status of valuable cuts of meat. Ninety-six 21-day-old chickens were assigned to four experimental diets (24/diet), with increasing doses of canola meal (CM 0, 2.5, 5 and 10%). Daily consumption, weekly live weight and post mortem carcass weight and yield were determined. At 24 h post mortem, pH, color (CIE L, a*, b*) and drip loss were measured in the breast, drumstick and thigh cuts. Fatty acid composition and health lipid indexes were also determined in the fresh cuts. The oxidative status of lipids and proteins, polyphenol and flavonoids content in fresh and in stored (7 days-display at 4–6 °C) in vacuum packaged cuts were determined. Including CM, up to 10%, the feed intake and growth of birds was not affected (p = 0.74 and p = 0.87 respectively). In meat, CM significantly decreased the drip loss (p < 0.05), the pH in breast and thigh (p = 0.01 and p = 0.05 respectively), a lower L and b in thigh and increased PUFAs in more oxidative cuts, with a strong interaction between dose and muscle type. There was no effect on lipid oxidation while carbonyls decrease at a 2.5% dose in fresh and stored cuts but there is an increase with higher ones. Flavonoids raise the maximum deposition in meat at 5% CM. In conclusion, CM can be included in finishing poultry diets, but high doses must to be adequately managed if performance and quality of meat criteria are considered together. Full article