Search Results (752)

In agricultural practice, improper irrigation levels and excessive fertiliser use negatively impact water resources and soil properties, respectively. This experiment aims to determine the effects of varying irrigation levels and vermicompost doses on the growth, quality, and productivity of pepper plants grown under polycarbonate greenhouse conditions. To achieve this objective, different irrigation levels (IL) of IL100 (100% full irrigation), IL75 (75%), IL50 (50%), and vermicompost doses (VD) of VD0 (0%), VD10 (10%), and VD20 (20%) were tested. The highest irrigation level was in the IL100–VD10 treatment, which also had the highest water consumption (ET) in the 27.8 L pot⁻¹. By comparison, the IL50–VD0 treatment had the lowest irrigation level in the 15.4 L pot⁻¹, representing nearly 55.4% of the maximum irrigation water amount. The findings showed that the irrigation levels and vermicompost doses had a significant impact on plant growth, quality, and fruit yield parameters. Accordingly, the irrigation levels and vermicompost doses had significant effects on the studied plant growth parameters (stem diameter, plant height, number of leaves, stem fresh weight, stem dry weight, root fresh weight, and root dry weight). Similar effects were also observed on the fruit quality parameters (fruit width, fruit length, fruit weight, fruit flesh thickness, pH, titratable acidity (TA), total soluble solids (TSS), chrome, and hue). This study found that the highest total yield (164.5 g pot⁻¹), marketable yield (149.8 g pot⁻¹), total water use efficiency (6.1 g L⁻¹), and marketable water use efficiency (5.6 g L⁻¹) were obtained at the 100% irrigation level. However, similar results were observed at the 75% irrigation level and a 20% vermicompost dose, where the total water use efficiency was 5.9 g L⁻¹ and the marketable water use efficiency was 5.3 g L⁻¹. This suggests that 75% irrigation can be a viable alternative to full irrigation (100%) and offers water-saving potential, particularly in areas with limited water resources. Full article

Background/Objectives: Family history is a known risk factor for multiple myeloma (MM) and its precursor condition, monoclonal gammopathy of undetermined significance (MGUS). Previous genome-wide association studies (GWASs) have identified 35 common loci associated with MM risk and 21 associated with MGUS. The objective of this study was to identify less common and rare genetic loci predisposing to MM/MGUS through whole-exome sequencing (WES)-based linkage analysis. Methods:Multipoint linkage analysis was conducted using the Multipoint Engine for Rapid Likelihood Inference (MERLIN) with the Lander–Green algorithm on germline WES data from 79 pedigrees with 2 or more affected relatives (120 MM, 86 MGUS, and 21 unaffected). Genome-wide linkage was evaluated using 12,946 independent single-nucleotide variants (linkage disequilibrium r² < 0.05). Results: Significant linkage was observed at chromosome 6q22.33–q24.2 by the non-parametric model (logarithm-of-odds (LOD) = 3.3) and suggestive linkage by the dominant parametric model (heterogeneity LOD (HLOD) = 2.5). Fourteen rare variants within this region were prioritized using family-specific partial LOD scores and in silico functional prediction tools. Nine of these variants, REPS1, THEMIS, TAAR6, AHI1, VNN1, VNN3, MTFR2/FAM54A, LAMA2, and PHACTR2, overlapped immune-regulatory regions in blood cell lines and were not previously identified in GWASs. Conclusions: This study demonstrates the utility of applying a linkage analysis framework to familial WES data for identifying genomic regions and candidate genes that may contribute to MM/MGUS predisposition. These findings provide new insight into the inherited risk and etiology of familial MM and MGUS. Full article

Lithium-ion batteries have been extensively utilized as a high-power, rechargeable, and dischargeable energy storage medium. Accurate estimation of the battery state of charge (SOC) in the battery management system (BMS) is imperative for ensuring the safe and stable operation of electric vehicles. This paper proposes an SOC estimation method based on the equivalent circuit model as well as the ampere-time integration method with a physical informed neural network. The network enhances the estimation of SOC by introducing two mechanistic information sources: the equivalent circuit model (ECM) and the ampere-time integration method (Ah-I method). These are utilized as a priori knowledge to constrain the estimation of SOC. Initially, the Rint model is selected as the physical analysis model of the lithium-ion battery, and subsequently, the Ah-I method is chosen as the auxiliary model for SOC output estimation. A deep learning network is then employed to establish the mapping between the battery input parameters and the SOC output. Finally, the SOC is estimated by fusing the physical model and the data-driven model. The results demonstrate the efficacy of the method in accurately estimating the state of charge of lithium batteries, with a root mean square error within 1%. The validity of the research methodology was further validated through comparison with other approaches. Full article

Plantar fasciitis (PFS) is a leading cause of heel pain, yet its clinical course varies widely. Although plantar fascia thickness (PFT) is often used as a pain marker, its prognostic value remains unclear. Objective: This study investigates whether foot arch morphology underlies distinct biomechanical profiles in PFS patients, potentially explaining the variability in its presentation. Methods: The cross-sectional study included 30 patients with PFS and 10 healthy controls. PFS patients were classified by arch type (pes rectus, pes planus, pes cavus) using the Arch Height Index (AHI). Baseline comparisons between healthy controls and PFS subgroups assessed PFT, Foot Function Index (FFI), joint stiffness ratio, and gait parameters. Results: PFT differed across groups but was not significantly associated with FFI scores (p = 0.233). The pes cavus group exhibited a lower metatarsophalangeal (MTP) stiffness ratio compared with healthy (p < 0.05). Pes planus and pes rectus groups showed excessive pronation, and the pes cavus group showed limited ankle dorsiflexion, indicating distinct gait mechanisms (p < 0.05). Conclusions: Foot arch morphology influences gait biomechanics, stiffness, and PFT in individuals with PFS. Incorporating individual arch types into clinical decision-making may facilitate more personalized interventions and improve treatment outcomes. Full article

Background: Obstructive sleep apnea (OSA) impacts daytime alertness, mood, cognition, and quality of life (QoL). Initial alterations in these patient-reported outcomes (PROs) following CPAP therapy, along with their association with adherence and residual respiratory events, are only partially understood. Materials and methods: We conducted a retrospective, observational study from January 2024 to May 2025 involving adult patients with OSA. Standardized assessments were performed at baseline and at six months following the initiation of CPAP: Epworth Sleepiness Scale (ESS), WHOQOL-BREF, MoCA, DASS-21, GAD-7, and PHQ-9. The primary endpoint was the change in Patient-Reported Outcomes (PROs) and cognitive performance. The second was to identify associations between these improvements and the degree of adherence to CPAP therapy and residual AHI. Results: Seventy-two patients (median age, 57; 65.3% male) with moderate to severe OSA had a baseline median AHI of 34.5/h, ODI of 35.5/h, and a mean nocturnal SpO₂ of 92.4%. The initial burden was high: median ESS was 14, indicating excessive daytime sleepiness (EDS), present in 68.9%; median MoCA was 24, with 98.6% scoring below 26; median PHQ-9 was 7; median GAD-7 was 5; and 56.8% and 47.9% scored below 50 in physical and psychological domains of WHOQOL-BREF, respectively. After 6 months, group averages showed improvement: ESS decreased to 8.6 ± 3.7, with a 27.0% residual EDS; PHQ-9 was 7.1 ± 4.5; GAD-7 was 6.2 ± 4.1; and MoCA increased to 25.3 ± 2.7, although 48.6% still showed impairment. WHOQOL-BREF scores improved across domains: physical 58.7 ± 14.2, psychological 61.5 ± 13.6, social 63.2 ± 15.4, and environmental 59.8 ± 14.7, with fewer scores below 50 (physical 23.0%, psychological 18.9%). CPAP adherence was high, with a mean of 87.7% and a median of 95%, predicting a greater ESS reduction (p = 0.027) and showing a trend toward improvement in PHQ-9 scores (ρ = 0.218; p = 0.066). Residual respiratory indices at 6 months (AHI, ODI, SpO₂) did not correlate with PRO or cognitive scores at the same time point (all p > 0.16), nor with their change scores. Conclusions: Over the course of six months, CPAP therapy led to notable improvements in sleepiness, mood, anxiety, cognition, and overall quality of life. Nonetheless, many patients continued to face residual problems, mainly excessive daytime sleepiness (EDS) and cognitive challenges. The positive effects were more closely associated with how well patients adhered to the treatment than with remaining levels of residual AHI or ODI. Full article

This study presents a comprehensive sustainability assessment of open field strawberry production in Batman, Türkiye, utilizing thermodynamic-based analyses. The inputs used during the production phase were obtained through survey data collected from local farms over the past few growing seasons. This study is the first to provide an integrated energy, exergy, and environmental assessment of open-field strawberry production in the Batman region, offering novel insights into resource efficiency and sustainability evaluation in agricultural systems. The evaluation was conducted for a functional unit of one ton of strawberries by analysing the cumulative energy consumption (CEnC), cumulative exergy consumption (CExC), and cumulative carbon dioxide emissions (CCO₂E). The total CEnC was calculated to be 1207.8 MJ/ton, with nitrogen fertilizer (390.91 MJ/ton) and diesel fuel (229.67 MJ/ton) being the most significant inputs. The total CExC was 1919.82 MJ/ton, where farmyard manure (1170.19 MJ/ton) was the dominant contributor, accounting for approximately 61% of the total. The total carbon footprint (CCO₂E) was determined to be 41.43 kg/ton, with irrigation water (10.19 kg/ton) and farmyard manure (10.14 kg/ton) being the primary sources. Key sustainability indicators, the Cumulative Degree of Perfection (CDP) and Renewability Indicator (RI), were calculated as 1.34 and 0.26, respectively. The CDP value is notably higher than that of other open field strawberry studies, while the low RI value indicates a heavy reliance on non-renewable resources. The findings highlight that improving sustainability requires a focus on efficient manure management and reducing fossil fuel-based energy consumption. Full article

Fish gelatins are increasingly recognized as sustainable biopolymers for food, packaging, and biomedical applications; however, their functional performance often requires improvement. In this study, the effects of transglutaminase (TG) modification on the physicochemical and structural properties of trout (T) and sea bass (SB) gelatins were systematically investigated. TG treatment enhanced gel strength in a species- and concentration-dependent manner, with trout increasing from 100 g (control) to 108 g at 0.04% TG and SB reaching a maximum of 163 g at 0.01% TG. Rheological measurements confirmed improved viscoelastic behavior, particularly in trout samples (G′ > G″). Emulsifying activity was optimized at 0.02–0.04% TG in trout, while excessive cross-linking reduced interfacial activity; nevertheless, emulsion stability was improved in both species. Foaming capacity and stability reached 195% and 148%, respectively, in trout, whereas higher TG concentrations led to reductions in SB foaming performance. Scanning electron microscopy revealed denser and more homogeneous networks in TG-modified gels, correlating with their enhanced rigidity. Transparency remained high, while zeta potential shifted toward more negative values, indicating improved colloidal stability. FTIR, UV–Vis, and DSC analyses confirmed conformational rearrangements and thermal stabilization after cross-linking. Minor decreases in oil-binding capacity and slight color changes were also observed. Overall, TG cross-linking significantly enhanced the functional and structural properties of fish gelatins in a source-dependent manner, supporting their potential as versatile and sustainable biopolymers for diverse industrial applications. Full article

Drought stress severely limits peanut productivity, highlighting the urgent need to understand the molecular mechanisms that underlie drought adaptation. While microRNAs (miRNAs) are known to play essential roles in plant stress responses, their functional contributions in polyploid crops like peanut remain insufficiently explored. This study provides the first integrated transcriptomic analysis of drought-responsive miRNAs in tetraploid peanut (Arachis hypogaea). We performed high-throughput sRNA sequencing on a drought-tolerant cultivar Fenhua 8 under PEG6000-simulated drought stress, identifying 10 conserved drought-responsive miRNAs. Among these, ahy-miR398 and ahy-miR408 were significantly downregulated under drought conditions. Degradome sequencing revealed that ahy-miR398 targets copper chaperones for superoxide dismutase (CCSs), potentially reducing SOD activation and amplifying oxidative stress. In contrast, ahy-miR408 targets laccase 12 (LAC12), P-type ATPase copper transporters (COPAs), and a blue copper protein-like (PCL) gene. These targets are involved in copper homeostasis and the regulation of reactive oxygen species (ROS), suggesting that ahy-miR408 plays a role in oxidative stress management. Functional validation in transgenic Arabidopsis lines overexpressing ahy-miR398 or ahy-miR408 showed significantly reduced drought tolerance, with impaired seed germination, shorter primary roots, and exacerbated growth suppression during water deprivation. Taken together, these findings highlight a novel miRNA-mediated regulatory network in peanut drought adaptation, centered on copper-associated oxidative stress management. This study provides new insights into miRNA-based regulation in polyploid crops and offers potential molecular targets for breeding climate-resilient peanut varieties, especially in arid regions where yield stability is crucial. Full article

The bidirectional relationship between obstructive sleep apnea (OSA) and type 2 diabetes mellitus (T2DM) represents a critical intersection in metabolic medicine. Therefore, the present review examines the most recent data regarding molecular mechanisms linking OSA and T2DM, analyzing key biomarkers including hypoxia-inducible factors (HIF 1α), inflammatory mediators, adipokines, microRNAs, hormones, and neuropeptides that serve as both diagnostic indicators and potential therapeutic targets. Key molecular findings from the scientific literature report elevated HIF-1α promoting insulin resistance, decreased SIRT1 levels, dysregulated microRNA-181a and microRNA-199a, increased inflammatory cytokines (TNF-α, IL-6, CRP), and altered adipokine profiles with reduced adiponectin and elevated leptin and resistin. Current clinical evidence reveals significant therapeutic potential for modern antidiabetic medications in the management of OSA. GLP-1 receptor agonists, particularly tirzepatide, received FDA approval as the first medication for moderate-to-severe OSA in obese adults, showing a 55–63% AHI reduction. SGLT2 inhibitors also demonstrate promising results through weight loss and cardiovascular protection mechanisms. This integrated approach represents the evolution toward comprehensive OSA management beyond traditional mechanical ventilation strategies. Future research should focus on developing personalized treatment algorithms based on individual molecular biomarker profiles, investigating combination therapies, and exploring novel targets, including chronotherapy agents. Full article

Background: Obstructive Sleep Apnea (OSA) is commonly treated with CPAP, though low patient compliance often limits its long-term use. Surgical alternatives, such as Barbed Pharyngoplasty, have been developed to address retro-velar collapse. A recent technique, Barbed Stayed Bridge Pharyngoplasty (BSBP), aims to preserve oropharyngeal anatomy while enhancing airway stability. This study evaluates the immediate postoperative outcomes and patient discomfort following BSBP for OSA. Material and Method: Thirty patients (mean age 40.7 ± 8.9 years; BMI 25.9 ± 1.7) underwent BSBP at Sapienza University of Rome between January 2022 and January 2024. Inclusion criteria included AHI 15–30, BMI ≤ 35, and specific DISE findings. Postoperative outcomes were evaluated using polysomnographic data (AHI, ODI), the Epworth Sleepiness Scale (ESS), pain scores (VAS), edema grading (Ezzat score), and the PPOPS questionnaire. Follow-ups were performed at 12, 24, and 48 h; 1 month; and 6 months postoperatively. Results: Thirty patients (average age 40.7) with mild to moderate OSA underwent surgery. After six months, there was a significant reduction in AHI (from 23.4 ± 2.1 to 7.2 ± 2.6) and ODI (from 21.0 ± 4.1 to 6.5 ± 2.1), along with a statistically significant improvement in the Epworth Sleepiness Scale. Postoperative pain (VAS) decreased from 3.0 ± 1.5 at 12 h to 0.2 ± 0.5 at one month, and edema (Ezzat score) decreased from 2.0 ± 0.6 at 12 h to 0.0 ± 0.0 at one month, both with significant differences. The PPOPS questionnaire scores remained relatively stable, with mean values of 4.9 ± 2.3 at 12 h, 2.7 ± 1.6 at 24 h, 1.2 ± 1.1 at 48 h, and 0.5 ± 0.7 at one month, showing statistically significant change (p < 0.005). No postoperative bleeding occurred. According to Sher’s criteria, the procedure was consistently effective. Conclusions: BSBP significantly reduces AHI and ODI, demonstrating effective symptom resolution with minimal discomfort and rapid recovery. These results suggest that BSBP may be a viable, less invasive surgical technique for OSA surgeries. Full article

Background/Objectives: C-reactive protein (CRP) is widely used as a marker of perioperative inflammation, but its predictive value for cardiac surgical outcomes remains uncertain. Obstructive sleep apnea (OSA), a prevalent and underrecognized comorbidity, may independently contribute to postoperative complications through non-inflammatory mechanisms. This study aimed to reevaluate the prognostic role of CRP and determine the clinical impact of OSA severity on postoperative recovery, focusing on new-onset atrial fibrillation (AF), prolonged intubation time, and postoperative CPAP/AIRVO use as indicators of respiratory burden. Methods: In this prospective cohort of 142 elective cardiac surgery patients, preoperative polysomnography and serial CRP measurements were obtained. Multivariable regression, mediation analysis, and propensity score matching (PSM) were performed to evaluate associations between OSA severity, CRP, and perioperative outcomes (AF, intubation time, CPAP/AIRVO use). Results: OSA severity independently predicted prolonged intubation (β = 1.74, p = 0.0019) and new-onset AF (β = 0.85, p = 0.004), even after excluding patients with preexisting arrhythmia. CRP showed poor discriminatory power as a standalone biomarker (AUC for IOT > 14 h = 0.445) and did not mediate OSA–outcome associations. However, CRP > 2.1 mg/dL doubled the odds of moderate-to-severe OSA (OR = 2.05, p = 0.041). A composite score integrating AHI, BMI, and postoperative CRP strongly correlated with postoperative respiratory support (p < 0.0001). Conclusions: OSA exerts a stronger and more consistent influence on perioperative outcomes than CRP, challenging reliance on CRP for risk stratification. Incorporating objective OSA screening and spirometry into preoperative assessment may enhance perioperative risk prediction and guide personalized management strategies. Full article

Peanut (Arachis hypogaea L.) is an important oil and cash crop, but its growth and productivity are severely constrained by low-temperature stress. Growth-regulating factors (GRFs) are plant-specific transcription factors involved in development and stress responses, yet their roles in peanut remain poorly understood. In this study, we identified AhGRF3b as a direct target of ahy-miR396 using degradome sequencing, which demonstrated precise miRNA-mediated cleavage sites within the AhGRF3b transcript. Expression profiling confirmed that ahy-miR396 suppresses AhGRF3b via post-transcriptional cleavage rather than translational repression. Functional analyses showed that overexpression of AhGRF3b in Arabidopsis thaliana promoted leaf expansion by enhancing cell proliferation. Specifically, leaf length, width, and petiole length increased by 104%, 22%, and 28%, respectively (p < 0.05). Under cold stress (0 °C for 7 days), transgenic lines (OE-2 and OE-6) exhibited significantly better growth than Col-0, with fresh weight increased by 158% and 146%, respectively (p < 0.05). Effect size analysis further confirmed these differences (Cohen’s d = 11.6 for OE-2 vs. Col-0; d = 6.3 for OE-6 vs. Col-0). Protein–protein interaction assays, performed using the yeast two-hybrid (Y2H) system and 3D protein–protein docking models, further supported that AhGRF3b interacts with Catalase 1 (AhCAT1), vacuolar cation/proton exchanger 3 (AhCAX3), probable polyamine oxidase 4 (AhPAO4), and ACT domain-containing protein 11 (AhACR11), which are involved in reactive oxygen species (ROS) scavenging and ion homeostasis. These interactions were associated with enhanced CAT and PAO enzymatic activities, reduced ROS accumulation, and upregulation of stress-related genes under cold stress. These findings suggest that the ahy-miR396/AhGRF3b module plays a potential regulatory role in leaf morphogenesis and cold tolerance, providing valuable genetic resources for breeding cold-tolerant peanut varieties. Full article

Background: Obstructive sleep apnea (OSA) is prevalent and often underdiagnosed in cardiology. Worldwide, approximately 936 million adults aged 30–69 are affected by OSA, with the highest numbers in the USA, China, Brazil, and India. In cardiovascular clinics, OSA is found in about 40–80% of patients with hypertension, heart failure, coronary artery disease, atrial fibrillation, or stroke. Meta-analyses link OSA to nearly twice the risk of cardiovascular disease, stroke, and all-cause mortality. Continuous positive airway pressure (CPAP) therapy addresses the underlying mechanisms of OSA and enhances intermediate cardiovascular indicators. Materials and Methods: We conducted a narrative review using major medical search engines (PubMed, Embase, Cochrane) to examine recent statements, meta-analyses, large cohorts, and key trials. The review focused on the cardiovascular burden of sleep apnea and its pathophysiology—including arrhythmic, hemodynamic, vascular, and coagulation aspects—as well as the effects of CPAP on intermediate cardiovascular outcomes. We aimed to provide a synthesised overview of current cardiovascular evidence related to the burden and mechanisms of OSA, and to summarise the effects of continuous positive airway pressure (CPAP) on intermediate and clinical cardiovascular outcomes. Results: Intermittent hypoxia, sleep fragmentation, and major negative fluctuations in intrathoracic pressure create a clear pathway leading to adverse cardiovascular outcomes. This occurs through mechanisms like sympathetic activation, RAAS activation, endothelial dysfunction, oxidative stress, and inflammation, linking OSA to these health issues. Studies show that greater severity of OSA correlates with higher cardiovascular risk, including increased incidence and recurrence of AF, resistant hypertension, and new cases of heart failure. CPAP effectively lowers AHI and enhances nocturnal oxygen levels, as well as intermediate cardiovascular indicators such as blood pressure, sympathetic activity, and certain aspects of ventricular function, with clinical benefits most evident in adherent patients. Conclusions: OSA is a significant, modifiable risk factor for cardiovascular disease. Routine cardiovascular care should include targeted screening for OSA, especially in cases of resistant hypertension, atrial fibrillation, and heart failure, along with timely sleep testing and adherence-focused CPAP therapy, in addition to traditional risk-reduction methods. Full article

Obstructive sleep apnea (OSA) is associated with increased risks of systemic comorbidities, leading to significant morbidity and mortality. This study investigates predictors of all-cause mortality, emphasizing the interplay of clinical symptoms, polysomnographic findings, and comorbidities. The aim of this study was to identify and compare respiratory predictors of all-cause mortality over 5, 10, and 15 years. A single-center study was conducted at a Sleep Medicine Department between 2005 and 2019, 4025 patients with suspected OSA who underwent polysomnography were admitted, 853 died during the study. We performed Cox regression analyses with dynamic hazard ratios to evaluated predictors of mortality. Prevalence of OSA was high—75.6% in the cohort: 929 patients with mild OSA (23.1%), 770 with moderate OSA (19.1%), and 1343 with severe OSA (33.4%). Survival rates were 89.7%, 81.9%, and 78.8% at 5, 10, and 15 years, respectively. Cardiovascular causes dominated mortality (33.3%), followed by cancer (26.5%). AHI_REM was associated with higher mortality risk in 0–5, 0–10, 0–15 years of observation in contrast to AHIN_REM and AHI_TST. The hazard ratio analysis showed that mortality risk changed over time depending on sleep stage and event type: risk increased for AHI_REM and AHI_TST, while it stayed the same or decreased for AHI_NREM and most central apneas. Full article

Objectives: This study examines the relationship between maxillary morphology and the severity of obstructive sleep apnea (OSA) in adults, with a focus on intermolar distance (IMD) and palatal height (PH) as predictive factors. Methods: A retrospective observational study was conducted at private orthodontic practices in Sydney, Australia. A total of 100 adults (50 OSA patients and 50 controls) were included. OSA diagnosis and severity were confirmed via hospital-based polysomnography. Digital maxillary models were analyzed using the Medit Link software (version 3.2.0; Medit Corp., Seoul, Republic of Korea), and IMD and PH were measured. Statistical analyses included one-way ANOVA and linear regression modeling, with adjustments for age and sex. Results: The OSA group exhibited significantly narrower IMD (28.96–35.01 mm) and higher PH (21.68–29.56 mm) compared to the control group (IMD: 36.35–41.50 mm; PH: 18.57–23.51 mm). A negative correlation was observed between IMD and PH across all groups. Linear regression analysis demonstrated a strong association between these craniofacial parameters and OSA severity (R² = 0.76, p < 0.001). IMD was negatively correlated with the Apnea-Hypopnea Index (AHI) (p = 0.003), while PH was positively correlated (p < 0.001). The inclusion of demographic variables did not significantly enhance the predictive model. Conclusions: Maxillary morphology associates with OSA severity, with narrower IMD and greater PH linked to higher AHI. Full article