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Life, Volume 10, Issue 5

May 2020 - 26 articles

Cover Story: Human articular chondrocytes (HACs) synthetize the extracellular matrix (ECM) that confer cartilage its biomechanical properties. HACs failure to produce a balanced ECM leads to osteoarthritis and inflammation worsen the disease. We have shown that miRNA miR-145 silences transcription factor Sox9 resulting in lower and higher levels, respectively, of the main ECM component Col2a1 and the catabolic protease MMP13. Here, by immunoprecipitating the miRNA-induced silencing complex (RISC) in HACs overexpressing miR-145, we identify more than 50 miR-145 new direct targets. One of these targets, DUSP6, contributes to MMP13 repression. Overexpression of miR-145 results in altered expression of hundreds of genes involved in ECM remodelling and immunity and inflammation. Thus, we propose miR-145 inhibition as a potential approach for cartilage repair. View this paper
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Articles (26)

  • Article
  • Open Access
42 Citations
6,379 Views
15 Pages

15 May 2020

Intrauterine adhesion (IUA), fibrosis, and scarring resulting from damage to the endometrium is a rare but serious clinical disease, contributing to a significant impairment of reproductive function. Uterine instrumentation, especially that of a hyst...

  • Article
  • Open Access
27 Citations
9,537 Views
95 Pages

14 May 2020

The Hesperornithiformes constitute the first known avian lineage to secondarily lose flight in exchange for the evolution of a highly derived foot-propelled diving lifestyle, thus representing the first lineage of truly aquatic birds. First unearthed...

  • Article
  • Open Access
17 Citations
4,591 Views
19 Pages

14 May 2020

Alzheimer’s disease (AD) is a progressive neurodegenerative disease characterized by a deficiency in cognitive skills. Although long noncoding RNAs (lncRNAs) have been proposed as associated with AD, the aberrant lncRNAs expression and the co-express...

  • Article
  • Open Access
22 Citations
4,139 Views
19 Pages

The Main Structural and Functional Characteristics of Photosystem-II-Enriched Membranes Isolated from Wild Type and cia3 Mutant Chlamydomonas reinhardtii

  • Vasily V. Terentyev,
  • Anna K. Shukshina,
  • Aleksandr A. Ashikhmin,
  • Konstantin G. Tikhonov and
  • Alexandr V. Shitov

14 May 2020

Photosystem II (PSII)-enriched membranes retain the original PSII architecture in contrast to PSII cores or PSII supercomplexes, which are usually isolated from Chlamydomonas reinhardtii. Here, we present data that fully characterize the structural a...

  • Article
  • Open Access
16 Citations
3,924 Views
17 Pages

Identification and Validation of Aerobic Adaptation QTLs in Upland Rice

  • Peng Xu,
  • Jun Yang,
  • Zhenbing Ma,
  • Diqiu Yu,
  • Jiawu Zhou,
  • Dayun Tao and
  • Zichao Li

14 May 2020

The aerobic adaptation of upland rice is considered as the key genetic difference between upland rice and lowland rice. Genetic dissection of the aerobic adaptation is important as the basis for improving drought tolerance and terrestrial adaptation...

  • Article
  • Open Access
7 Citations
4,294 Views
14 Pages

DNA Methylation Influences miRNA Expression in Gonadotroph Pituitary Tumors

  • Joanna Boresowicz,
  • Paulina Kober,
  • Natalia Rusetska,
  • Maria Maksymowicz,
  • Agnieszka Paziewska,
  • Michalina Dąbrowska,
  • Natalia Zeber-Lubecka,
  • Jacek Kunicki,
  • Wiesław Bonicki and
  • Jerzy Ostrowski
  • + 2 authors

13 May 2020

microRNAs are involved in pathogenesis of cancer. DNA methylation plays a role in transcription of miRNA-encoding genes and may contribute to changed miRNA expression in tumors. This issue was not investigated in pituitary neuroendocrine tumors (PitN...

  • Article
  • Open Access
23 Citations
5,539 Views
17 Pages

Trehalose Effect on The Aggregation of Model Proteins into Amyloid Fibrils

  • Eleonora Mari,
  • Caterina Ricci,
  • Silvia Pieraccini,
  • Francesco Spinozzi,
  • Paolo Mariani and
  • Maria Grazia Ortore

13 May 2020

Protein aggregation into amyloid fibrils is a phenomenon that attracts attention from a wide and composite part of the scientific community. Indeed, the presence of mature fibrils is associated with several neurodegenerative diseases, and in addition...

  • Article
  • Open Access
6 Citations
3,496 Views
10 Pages

Loss of Concentration May Occur by Blink Inhibition in DED Simulation Models

  • Yasue Mitsukura,
  • Kazuno Negishi,
  • Masahiko Ayaki,
  • Mayuko Santo,
  • Motoko Kawashima and
  • Kazuo Tsubota

13 May 2020

Purpose: Patients with dry eye disease (DED) often suffer productivity loss and distress due to bothersome symptoms. The aim of this study was to objectively quantify and compare productivity-related emotional states obtained from brain waveforms in...

  • Article
  • Open Access
13 Citations
4,243 Views
22 Pages

High-Throughput Identification of MiR-145 Targets in Human Articular Chondrocytes

  • Aida Martinez-Sanchez,
  • Stefano Lazzarano,
  • Eshita Sharma,
  • Helen Lockstone and
  • Christopher L. Murphy

11 May 2020

MicroRNAs (miRNAs) play key roles in cartilage development and homeostasis and are dysregulated in osteoarthritis. MiR-145 modulation induces profound changes in the human articular chondrocyte (HAC) phenotype, partially through direct repression of...

  • Review
  • Open Access
13 Citations
4,840 Views
17 Pages

Targeting the C-Terminal Domain Small Phosphatase 1

  • Harikrishna Reddy Rallabandi,
  • Palanivel Ganesan and
  • Young Jun Kim

8 May 2020

The human C-terminal domain small phosphatase 1 (CTDSP1/SCP1) is a protein phosphatase with a conserved catalytic site of DXDXT/V. CTDSP1’s major activity has been identified as dephosphorylation of the 5th Ser residue of the tandem heptad repe...

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Life - ISSN 2075-1729