Table of Contents
Life, Volume 10, Issue 5 (May 2020) – 26 articles
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Cover Story (view full-size image) Human articular chondrocytes (HACs) synthetize the extracellular matrix (ECM) that confer cartilage [...] Read more. Human articular chondrocytes (HACs) synthetize the extracellular matrix (ECM) that confer cartilage its biomechanical properties. HACs failure to produce a balanced ECM leads to osteoarthritis and inflammation worsen the disease. We have shown that miRNA miR-145 silences transcription factor Sox9 resulting in lower and higher levels, respectively, of the main ECM component Col2a1 and the catabolic protease MMP13. Here, by immunoprecipitating the miRNA-induced silencing complex (RISC) in HACs overexpressing miR-145, we identify more than 50 miR-145 new direct targets. One of these targets, DUSP6, contributes to MMP13 repression. Overexpression of miR-145 results in altered expression of hundreds of genes involved in ECM remodelling and immunity and inflammation. Thus, we propose miR-145 inhibition as a potential approach for cartilage repair. View this paper