Diversity

37 pages, 35096 KiB

Open AccessEditor’s ChoiceArticle

Seaweed-Associated Diatoms (Bacillariophyta) in Dokdo of South Korea: I. Subphyla Melosirophytina, Coscinodiscophytina, and Class Mediophyceae

by Joon Sang Park, Kyun-Woo Lee, Seung Won Jung, Han Jun Kim and Jin Hwan Lee

Diversity 2024, 16(11), 690; https://doi.org/10.3390/d16110690 - 12 Nov 2024

Viewed by 1533

Abstract

Dokdo is an island located in the easternmost part of Korea, which has high levels of biodiversity of birds and fish, especially marine invertebrates. However, the biodiversity of microalgae, especially diatoms (Bacillariophyta), is relatively unknown, despite their ecological importance as primary producers of [...] Read more.

Dokdo is an island located in the easternmost part of Korea, which has high levels of biodiversity of birds and fish, especially marine invertebrates. However, the biodiversity of microalgae, especially diatoms (Bacillariophyta), is relatively unknown, despite their ecological importance as primary producers of the marine food web and bioindicators of environmental conditions associated with climate change. To understand the biodiversity of seaweed-associated diatoms from Dokdo, we collected macroalgae present at a depth 5–15 m by SCUBA diving on 17 October 2017. There were a large number of diatoms (over 130 species), even though it was a one-time survey. As it includes too many taxa to cover at once, voucher flora for other taxonomic groups will be provided through the continuous serial papers. This is the first series of seaweed-associated diatoms, with 26 species belonging to the subphyla Melosirophytina and Coscinodisophytina, and the class Mediophyceae. Among these, seven species including one new taxon were reported for the first time in Korea, which, along with the geopolitical characteristics of the survey area, proved that there is no domestic interest in seaweed-related diatoms. In particular, the appearance of species that have been reported in subtropical waters, such as the order Ardissoneales, requires continuous monitoring of marine seaweed-associated diatoms to confirm whether their colonization in Dokdo waters was due to climate change or species-specific water temperature tolerance. Full article

(This article belongs to the Section Marine Diversity)

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14 pages, 1877 KiB

Open AccessEditor’s ChoiceArticle

Description of Oryzobacter telluris sp. nov., a New Species Isolated from Bank-Side Soil in Seomjin River, South Korea

by Ahyoung Choi, Sumin Jang and Jaeduk Goh

Diversity 2024, 16(11), 689; https://doi.org/10.3390/d16110689 - 12 Nov 2024

Cited by 1 | Viewed by 979

Abstract

A novel bacterial strain, designated 24SJ04S-52^T, was isolated from bank-side soil in the Osucheon Stream of the Seomjin River, Republic of Korea. This strain is aerobic, Gram-stain-positive, and short-rod-shaped, with optimal growth observed at 30 °C, pH 7, and 0% salinity, [...] Read more.

A novel bacterial strain, designated 24SJ04S-52^T, was isolated from bank-side soil in the Osucheon Stream of the Seomjin River, Republic of Korea. This strain is aerobic, Gram-stain-positive, and short-rod-shaped, with optimal growth observed at 30 °C, pH 7, and 0% salinity, and growth occurring across a temperature range of 15–37 °C, pH 5–9, and salinity of 0–4%. Phylogenetic analysis of the 16S rRNA gene showed that strain 24SJ04S-52^T shares 98.3% sequence similarity with Oryzobacter terrae PSGM2-16^T. However, the average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values were 85.0% and 50.1%, respectively, which are well below the species delineation thresholds of 95–96% for ANI and 70% for dDDH, confirming the novelty of this species. Genomic analysis identified a genome size of 3.98 Mb with a G+C content of 72.9 mol%. Functional annotation revealed various genes involved in amino acid, carbohydrate, and protein metabolism, suggesting metabolic versatility that may support adaptation to nutrient-variable environments. Chemotaxonomic analyses revealed distinctive profiles, including major fatty acids such as C_17:1 ω8c, iso-C_16:0, and iso-C_14:0, with MK-8(H₄) as the predominant menaquinone. The polar lipids included diphosphatidylglycerol, phosphatidylethanolamine, and phosphatidylinositol, and the peptidoglycan was of type A4γ with meso-diaminopimelic acid as the diagnostic diamino acid. These comprehensive analyses support the classification of strain 24SJ04S-52^T as a novel species within the genus Oryzobacter, for which the name Oryzobacter telluris sp. nov. is proposed. The type strain is 24SJ04S-52^T (=KACC 23836^T = FBCC-B16192^T). Full article

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14 pages, 7720 KiB

Open AccessEditor’s ChoiceArticle

First Attempts at DNA Barcoding Lepidoptera in North Cyprus Reveal Unexpected Complexities in Taxonomic and Faunistic Issues

by Peter Huemer and Özge Özden

Diversity 2024, 16(11), 671; https://doi.org/10.3390/d16110671 - 31 Oct 2024

Viewed by 1264

Abstract

The fauna of Lepidoptera in the Mediterranean is still inadequately documented. As a result, even remotely complete DNA barcode libraries (mt. COI (cytochrome c oxidase 1) gene) are lacking in most areas. This proposed gap is being analyzed for the first time for [...] Read more.

The fauna of Lepidoptera in the Mediterranean is still inadequately documented. As a result, even remotely complete DNA barcode libraries (mt. COI (cytochrome c oxidase 1) gene) are lacking in most areas. This proposed gap is being analyzed for the first time for the fauna of North Cyprus. In the initial phase, 248 morphospecies from 29 families (exclusive Heterocera) were sampled, sequenced and compared with existing DNA reference sequences in the global BOLD database (Barcode of Life Data Systems) via BINs (Barcode Index Numbers). A total of 194 species could be unequivocally assigned to a Linnaean taxon. Additionally, six species previously unidentified in BOLD, as well as fourteen species without reference barcodes, were identified at the species level. Twenty-four of these species were new records for Cyprus. In addition, 25 taxa with new BINs could not be assigned to a valid species due to potential cryptic diversity or the lack of relevant revisions. Furthermore, a few species could not be identified due to barcode sharing and/or potential misidentifications in BOLD. Overall, approximately 20% of the samples could not be identified using the existing DNA barcode libraries, a significant deficit for European standards, which should be addressed as a priority issue in future studies. Full article

(This article belongs to the Special Issue DNA Barcodes for Evolution and Biodiversity—2nd Edition)

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16 pages, 12044 KiB

Open AccessEditor’s ChoiceArticle

Integrative Systematics Reveals Cryptic Diversity in Paraphrynus Whip Spiders (Amblypygi: Phrynidae) from Southwestern North America

by Nicolas Cazzaniga and Lorenzo Prendini

Diversity 2024, 16(11), 669; https://doi.org/10.3390/d16110669 - 30 Oct 2024

Viewed by 2632

Abstract

Due to their continuous growth, reclusive nature, and low vagility, the distributions and species limits of many whip spiders (Amblypygi Thorell, 1883) remain poorly understood, and much cryptic diversity remains unrecognized. Past attempts to separate the historical “forms” of Paraphrynus Moreno, 1940 into [...] Read more.

Due to their continuous growth, reclusive nature, and low vagility, the distributions and species limits of many whip spiders (Amblypygi Thorell, 1883) remain poorly understood, and much cryptic diversity remains unrecognized. Past attempts to separate the historical “forms” of Paraphrynus Moreno, 1940 into morphologically diagnosable species resulted, for example, in the division of Paraphrynus mexicanus (Bilimek, 1867) into three species—the nominotypical form, Paraphrynus cubensis Quintero, 1983, and Paraphrynus carolynae Armas, 2012. Nevertheless, the limitations of conservative morphology continue to hinder progress towards clarifying the diversity of Paraphrynus. One such example concerns P. carolynae, distributed from Arizona to central Mexico as currently defined. Through the acquisition of new, freshly collected material, the discovery of novel morphological characters, and molecular systematics analyses, it became apparent that P. carolynae comprises at least two morphologically diagnosable species. In this present contribution, the northernmost population of P. carolynae occurring in Arizona and California is described as a new species, Paraphrynus tokdod, sp. nov., raising the number of species in the genus to 22. This investigation also revealed more variation than expected in the secondary spine counts of the pedipalps and the trichobothrial counts of leg IV, previously used for species delimitation in Paraphrynus, suggesting that such characters should be used with caution. Full article

(This article belongs to the Special Issue Diversity in 2024)

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19 pages, 3754 KiB

Open AccessEditor’s ChoiceArticle

The Vulnerability of Malagasy Protected Areas in the Face of Climate Change

by Qi Lai and Carl Beierkuhnlein

Diversity 2024, 16(11), 661; https://doi.org/10.3390/d16110661 - 27 Oct 2024

Viewed by 1536

Abstract

This study examines the vulnerability of Madagascar’s protected areas (PAs) to climate change, focusing on climate change velocity, and its impact on biodiversity. We analyzed current and near future climate data using principal component analysis (PCA) and climate change velocity metrics to predict [...] Read more.

This study examines the vulnerability of Madagascar’s protected areas (PAs) to climate change, focusing on climate change velocity, and its impact on biodiversity. We analyzed current and near future climate data using principal component analysis (PCA) and climate change velocity metrics to predict shifts in climatic conditions from the present to the near future, while under the mild and extreme emission scenarios (SSP 126, SSP 585). Forward velocities, which are characterized by the minimum distances that must be overcome by species to keep in track with their appropriate comparative climate, are most pronounced in western and southern Madagascar. In contrast, the backward velocity, which uses future climatic conditions in grid cells in comparison to current conditions, is more common in the eastern regions of the island, and hints at the minimum distance that organisms would have to overcome in colonizing a new habitat. Even though the correlations between PA size and climate change velocity are weak, there is a tendency for larger PAs to exhibit more stable climatic conditions. Conservation strategies must prioritize enhancing the resilience of PAs through adaptive management to mitigate climate impacts. Our findings provide crucial insights for policymakers and conservation planners to develop climate-smart strategies that ensure the long-term efficacy of Madagascar’s PA network. Full article

(This article belongs to the Special Issue 2024 Feature Papers by Diversity’s Editorial Board Members)

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12 pages, 664 KiB

Open AccessEditor’s ChoiceArticle

Habitat Fragmentation Affects Richness—A View Through a Metacommunity Lens

by Jessica Marchesan and Jurek Kolasa

Diversity 2024, 16(11), 658; https://doi.org/10.3390/d16110658 - 25 Oct 2024

Viewed by 1762

Abstract

Habitat fragmentation is often assumed to negatively impact species diversity because smaller, more isolated populations on smaller habitat patches are at a higher extinction risk. However, some empirical and theoretical studies suggest that landscapes with numerous small habitat patches may support higher species [...] Read more.

Habitat fragmentation is often assumed to negatively impact species diversity because smaller, more isolated populations on smaller habitat patches are at a higher extinction risk. However, some empirical and theoretical studies suggest that landscapes with numerous small habitat patches may support higher species richness, although the circumstances remain elusive. We used an agent-based metacommunity model to investigate this and simulate landscapes of the same total area but diverse patch sizes. Our model, as generic and unbiased by specific assumptions as possible, aimed to explore which circumstances may be more conducive to supporting higher biodiversity. To this end, most parameters and behaviors were random. The model included generalized species traits, dispersal, and interactions to explore species richness dynamics in fragmented landscapes of distinct patch sizes. Our results show that landscapes with many small patches maintain higher species richness than those with fewer large patches. Moreover, the relationship between patch connectivity and species richness is more pronounced in landscapes with smaller patches. High connectivity in these landscapes may support species diversity by preventing local extinctions and facilitating recolonization. In contrast, connectivity is less significant in large-patch landscapes, where generalist species dominate. The findings highlight the complex interplay between patch size quality, connectivity, species traits, and diverse interactions among species in determining species richness. We suggest the patterns produced by the model represent null predictions and may be useful as a reference for a diversity of more specialized questions and predictions. These insights may also have specific implications for conservation strategies, suggesting that maintaining a mosaic of small, well-connected patches could enhance biodiversity in fragmented landscapes. Full article

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27 pages, 4549 KiB

Open AccessEditor’s ChoiceArticle

Benthic Community Metrics Track Hydrologically Stressed Mangrove Systems

by Amanda W. J. Demopoulos, Jill R. Bourque, Jennifer P. McClain-Counts, Nicole Cormier and Ken W. Krauss

Diversity 2024, 16(11), 659; https://doi.org/10.3390/d16110659 - 25 Oct 2024

Viewed by 1386

Abstract

Mangrove restoration efforts have increased in order to help combat their decline globally. While restoration efforts often focus on planting seedlings, underlying chronic issues, including disrupted hydrological regimes, can hinder restoration success. While improving hydrology may be more cost-effective and have higher success [...] Read more.

Mangrove restoration efforts have increased in order to help combat their decline globally. While restoration efforts often focus on planting seedlings, underlying chronic issues, including disrupted hydrological regimes, can hinder restoration success. While improving hydrology may be more cost-effective and have higher success rates than planting seedlings alone, hydrological restoration success in this form is poorly understood. Restoration assessments can employ a functional equivalency approach, comparing restoration areas over time with natural, reference forests in order to quantify the relative effectiveness of different restoration approaches. Here, we employ the use of baseline community ecology metrics along with stable isotopes to track changes in the community and trophic structure and enable time estimates for establishing mangrove functional equivalency. We examined a mangrove system impacted by road construction and recently targeted for hydrological restoration within the Rookery Bay National Estuarine Research Reserve, Florida, USA. Samples were collected along a gradient of degradation, from a heavily degraded zone, with mostly dead trees, to a transition zone, with a high number of saplings, to a full canopy zone, with mature trees, and into a reference zone with dense, mature mangrove trees. The transition, full canopy, and reference zones were dominated by annelids, gastropods, isopods, and fiddler crabs. Diversity was lower in the dead zone; these taxa were enriched in ¹³C relative to those found in all the other zones, indicating a shift in the dominant carbon source from mangrove detritus (reference zone) to algae (dead zone). Community-wide isotope niche metrics also distinguished zones, likely reflecting dominant primary food resources (baseline organic matter) present. Our results suggest that stable isotope niche metrics provide a useful tool for tracking mangrove degradation gradients. These baseline data provide critical information on the ecosystem functioning in mangrove habitats following hydrological restoration. Full article

(This article belongs to the Special Issue Mangrove Regeneration and Restoration)

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20 pages, 2886 KiB

Open AccessEditor’s ChoiceArticle

Age Difference, Not Food Scarcity or Sibling Interactions, May Drive Brood Reduction in Wild Scarlet Macaws in Southeastern Peru

by Gabriela Vigo-Trauco, Gustavo Martínez-Sovero and Donald J. Brightsmith

Diversity 2024, 16(11), 657; https://doi.org/10.3390/d16110657 - 24 Oct 2024

Viewed by 2421

Abstract

Avian brood reduction was initially thought to be driven by insufficient food supply. Now it is more commonly considered a consequence of asynchronous hatching and resulting siblicide, direct filial infanticide (where parents kill specific chicks) or indirect filial infanticide (where parents starve specific [...] Read more.

Avian brood reduction was initially thought to be driven by insufficient food supply. Now it is more commonly considered a consequence of asynchronous hatching and resulting siblicide, direct filial infanticide (where parents kill specific chicks) or indirect filial infanticide (where parents starve specific chicks). In psittacines, brood reduction has been reported, but the mechanisms and causes remain unexplored. In this paper, we test the hypotheses that Scarlet Macaw chick starvation is driven by (1) sibling aggression, (2) food scarcity, and (3) parental food allocation based on (a) chick hatch weight and (b) chick age differences. We documented wild Scarlet Macaw behavior in lowland Peru in 37 nests over 19 seasons using morphological measurements and nest videos. Chick starvation was the leading cause of chick mortality (27% of all second-hatched chicks starve, and nearly all third- and fourth-hatched chicks starve). We found no evidence that starvation was caused by (1) sibling conflicts or (2) food availability. We did find parental food distribution favors first-hatched chicks, with larger age differences increasing the chances of second chick starvation. This study offers insights into brood reduction among Neotropical cavity-nesting birds and enhances our capacity to develop scientifically informed management strategies to support endangered psittacines. Full article

(This article belongs to the Special Issue Ecology and Conservation of Parrots)

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16 pages, 835 KiB

Open AccessEditor’s ChoiceArticle

Diversity and Distribution of Australian Stygobiont and Other Groundwater-Associated Amphipods (Crustacea: Malacostraca: Peracarida)

by Rachael A. King, Steven J. B. Cooper, Benjamin Schwartz, Remko Leijs, Danielle N. Stringer, William F. Humphreys, Jake Thornhill and Michelle T. Guzik

Diversity 2024, 16(10), 650; https://doi.org/10.3390/d16100650 - 21 Oct 2024

Viewed by 1292

Abstract

Numerous and diverse groundwater habitats suitable for sustaining aquatic invertebrate communities exist across Australia. These habitats include enclosed subterranean aquifer systems, fractured rock, alluvial aquifers, perched aquifers, artesian springs, and spring-fed seeps and marshes. Crustaceans are a dominant member of these groundwater-associated invertebrate [...] Read more.

Numerous and diverse groundwater habitats suitable for sustaining aquatic invertebrate communities exist across Australia. These habitats include enclosed subterranean aquifer systems, fractured rock, alluvial aquifers, perched aquifers, artesian springs, and spring-fed seeps and marshes. Crustaceans are a dominant member of these groundwater-associated invertebrate communities, and amphipods, both stygobiont and associated epigean species, are particularly diverse yet are still relatively poorly known. We review both the diversity and distributions of Australian amphipods associated with groundwater habitats, describing hotspots of diversity, providing notes on the unique Australian habitats, and examining the extraordinary species diversity and endemism of the Australian species. Our review highlights the significance of Australian groundwater ecosystems, their associated biodiversity, and the importance in considering these ecosystems in groundwater conservation management plans. Full article

(This article belongs to the Special Issue Diversity and Evolution within the Amphipoda)

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12 pages, 2745 KiB

Open AccessEditor’s ChoiceArticle

Early Molecular Detection of Invasive Alien Plants in Urban and Peri-Urban Areas

by Jessica Frigerio, Malika Ouled Larbi, Werther Guidi Nissim, Fabrizio Grassi, Pierluigi Cortis and Massimo Labra

Diversity 2024, 16(10), 647; https://doi.org/10.3390/d16100647 - 19 Oct 2024

Cited by 1 | Viewed by 1404

Abstract

Invasive alien plants represent one of the five major threats to biodiversity and the disruption of ecosystems. They are introduced through various routes, starting with commercial trade. Preventing their introduction is essential to avoid the spread of new invasive plants. In this paper, [...] Read more.

Invasive alien plants represent one of the five major threats to biodiversity and the disruption of ecosystems. They are introduced through various routes, starting with commercial trade. Preventing their introduction is essential to avoid the spread of new invasive plants. In this paper, we propose a new early warning DNA barcoding tool for invasive plant detection. Eight invasive alien species of European Union concern (i.e., Ludwigia grandiflora, Elodea nuttallii, Myriophyllum aquaticum, Pontederia crassipes, Ailanthus altissima, Heracleum mantegazzianum, Impatiens glandulifera, Pueraria montana) were selected and analysed. A unique DNA marker for each species was identified and amplified using species-specific primers capable of identifying the presence of alien species. To verify whether the approach could detect the presence of alien plants in urban areas from lawn clippings, mixes with typical urban spontaneous plants and invasive species were tested. In all mixes, only the invasive species was identified. This rapid detection capability will enable environmental operators to intervene promptly to contain the spread of invasive plants before they can cause significant damage to the local ecosystem. This tool could have a significant impact on the protection of local biodiversity and the integrity of urban habitats. Full article

(This article belongs to the Special Issue DNA Barcoding for Biodiversity Conservation and Restoration)

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18 pages, 2479 KiB

Open AccessEditor’s ChoiceArticle

Zoonotic Pathogens Isolated from an Introduced Population of Red Swamp Crayfish (Procambarus clarkii) in Tenerife (Canary Islands, Spain)

by Néstor Abreu-Acosta, Natalia Martín-Carrillo and Pilar Foronda

Diversity 2024, 16(10), 643; https://doi.org/10.3390/d16100643 - 16 Oct 2024

Viewed by 2136

Abstract

The red swamp crayfish (Procambarus clarkii) is a widely distributed invasive species that is listed in the Delivering Alien Invasive Species Inventory for Europe. Native to North America, it has been introduced to numerous regions, such as the Canary Islands, Spain. [...] Read more.

The red swamp crayfish (Procambarus clarkii) is a widely distributed invasive species that is listed in the Delivering Alien Invasive Species Inventory for Europe. Native to North America, it has been introduced to numerous regions, such as the Canary Islands, Spain. Previous studies have confirmed the role of this crayfish in the maintenance of several foodborne pathogenic bacteria. Therefore, the aim of this study was to analyze the main zoonotic bacterial and parasitic pathogens present in a P. clarkii population introduced to the island of Tenerife, Canary Islands, and to assess the potential risk to public health and native fauna. A total of 22 crayfish from Tenerife were analyzed using Biofire FilmArray Gastrointestinal Panels and culture–PCR methods. The results show the presence of Plesiomonas shigelloides, Shigella/enteroinvasive Escherichia coli, enteropathogenic Escherichia coli, Salmonella ser. Enteritidis, Salmonella ser. Typhimurium, and Salmonella ser. Typhi. These results demonstrate the presence of a variety of pathogenic bacteria in the red swamp crayfish in Tenerife that represent a significant concern in terms of public health and conservation. Implementing educational campaigns to inform the community about the risks associated with handling and consuming contaminated crayfish, as well as initiatives for the restoration of the contaminated ecosystem, are necessary to prevent the transmission of the foodborne pathogens. Full article

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21 pages, 12788 KiB

Open AccessEditor’s ChoiceArticle

A Multi-Scale Species Distribution Model for Migrating and Overwintering Western Monarch Butterflies: Climate Is the Best Predictor

by Ashley R. Fisher, William T. Bean and Francis X. Villablanca

Diversity 2024, 16(10), 640; https://doi.org/10.3390/d16100640 - 15 Oct 2024

Cited by 2 | Viewed by 1570

Abstract

Western Monarch butterflies (Danaus plexippus) migrate from inland breeding ranges to coastal overwintering grounds in California. Given that migratory individuals may make multi-scale habitat selection decisions, we considered a multi-scale species distribution model (SDM) using range-wide climatic and local landscape-level predictors of [...] Read more.

Western Monarch butterflies (Danaus plexippus) migrate from inland breeding ranges to coastal overwintering grounds in California. Given that migratory individuals may make multi-scale habitat selection decisions, we considered a multi-scale species distribution model (SDM) using range-wide climatic and local landscape-level predictors of migratory and overwintering habitat and community-science presence data. The range-wide model output was included as a predictor in the local-scale model, generating multi-scale habitat suitability. The top range-wide predictor was the minimum temperature in December, contributing 83.7% to the model, and was positively associated with presence. At the local scale, the strongest predictors of presence were the range-wide output and percent coverage of low and medium levels of development, contributing > 95%, with 61–63% from the range-wide output, with local-scale suitability coinciding with the California coastal zones. Development’s positive association with overwintering monarch presence was counterintuitive. It is likely that our local-scale model is overfit to these development zones, but it is unclear whether this overfitting resulted from modeler choices, monarchs overwintering close to human development, biased detection near human development, or a combination of these factors. Therefore, alternative approaches to collecting local-scale attribute data are suggested while recognizing the primacy of climate in restricting overwinter sites. Full article

(This article belongs to the Collection Feature Papers in Animal Diversity)

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18 pages, 5341 KiB

Open AccessEditor’s ChoiceArticle

Prevalence and Diversity of Plant Parasitic Nematodes in Irish Peatlands

by Anusha Pulavarty, Tilman Klappauf, Ankit Singh, Patricia Molero Molina, Anique Godjo, Bastiaan Molleman, Douglas McMillan and Thomais Kakouli-Duarte

Diversity 2024, 16(10), 639; https://doi.org/10.3390/d16100639 - 15 Oct 2024

Viewed by 1399

Abstract

The prevalence of plant parasitic nematodes (PPN) in the Irish peatlands was investigated in five different peatland habitats—raised bog, cutover scrub/woodlands, fens and peat grasslands, which were further sub-categorised into fourteen different sub-habitats. Within the raised bog habitat were healthy bog hummock (HBH), [...] Read more.

The prevalence of plant parasitic nematodes (PPN) in the Irish peatlands was investigated in five different peatland habitats—raised bog, cutover scrub/woodlands, fens and peat grasslands, which were further sub-categorised into fourteen different sub-habitats. Within the raised bog habitat were healthy bog hummock (HBH), healthy bog lawn (HBL), degraded bog hummock (DBH) and degraded bog lawn (DBL) and the fen habitats were fen peat (FP) and rich fen peat (R-FP). Cutover scrub or woodland habitat included cutover scrub rewetted (C-RW), cutover scrub non-rewetted (C-NRW), woodlands rewetted (W-RW) and woodlands non-rewetted (W-NRW). Grassland included wasted peat (WP), rough grazing (RG-I) and improved fen peat grassland (IFPG-RW and IFPG-NRW). Soil samples from peatlands were all collected between July and December 2023 when the temperature ranged from 12 to 20 °C. One half of each sample was used for molecular nematode analysis and the other half for morphological identification of nematodes. For the morphological identification, a specific nematode extraction protocol was optimised for peatland soils, and the extracted nematodes were fixed onto slides to be studied under a high-power light microscope. Subsequently, the other part of the soil was processed to isolate total DNA, from which the 18S rRNA gene was sequenced for the identification of nematode taxa. The extracted DNA was also used for randomly amplified polymorphic DNA (RAPD) fingerprinting analysis to determine banding patterns that could classify different bog habitats based on PPN random primers. Compared to that in the climax habitats (HBH, HBL, DBH, DBL, FP, R-FP), PPN prevalence was recorded as being higher in grasslands (WP, RG-I, IFPG-RW and IFPG-NRW) and scrub/woodland ecosystems (C-RW, C-NRW, W-RW, W-NRW). The results indicate that nematode populations are different across the various bog habitats. Emerging and current quarantine PPN belonging to the families Pratylenchidae, Meloidogynidae, Anguinidae and Heteroderidae were noted to be above the threshold limits mentioned under EPPO guidelines, in grassland and wooded peatland habitats. Future actions for PPN management may need to be considered, along with the likelihood that these PPN might impact future paludiculture and other crops and trees growing in nearby agricultural lands. Full article

(This article belongs to the Section Biodiversity Conservation)

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21 pages, 1795 KiB

Open AccessEditor’s ChoiceArticle

Geographical Variation in the Sand Cat, Felis margarita (Carnivora: Felidae)

by Andrew C. Kitchener, Alexei V. Abramov, Géraldine Veron, Lisa Banfield, Helen Senn, Nobuyuki Yamaguchi and Andrey Yu. Puzachenko

Diversity 2024, 16(10), 635; https://doi.org/10.3390/d16100635 - 11 Oct 2024

Cited by 1 | Viewed by 3997

Abstract

Sand cats, Felis margarita, range from northern Africa and the Arabian Peninsula to Central Asia. Their apparently discontinuous distribution is recognized as comprising four subspecies. Recent genetic research found little differentiation between subspecies except for the North African form. In this study, [...] Read more.

Sand cats, Felis margarita, range from northern Africa and the Arabian Peninsula to Central Asia. Their apparently discontinuous distribution is recognized as comprising four subspecies. Recent genetic research found little differentiation between subspecies except for the North African form. In this study, 90 skins and 88 skulls were analyzed from the four subspecies. A discriminant function analysis of the scores, ranging from 1 to 4, of four pelage characteristics revealed differentiation between putative subspecies, except between Turkmenian and Pakistani sand cats. Northern African and Arabian sand cats tend to be spotted and striped, while Turkmenian and Pakistani sand cats are less spotted and have a dorsal crest of fur. Nonmetric multidimensional scaling (NMDS) models generated from 21 skull measurements revealed an overlap in morphospace between all subspecies, except for larger Turkmenian sand cats; northern African sand cats were smallest. Therefore, both pelage characteristics and skull morphometrics support up to three subspecies. However, considering recent genetic research, it is likely that two subspecies should be recognized, F. m. margarita from northern Africa and F. m. thinobia from the Arabian Peninsula, and Southwest and Central Asia. Widening of the dataset and nuclear DNA evidence are required to increase our understanding of geographical variation in this little studied species. Full article

(This article belongs to the Special Issue Ecology and Evolution of Mammals)

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21 pages, 29272 KiB

Open AccessEditor’s ChoiceArticle

Terrestrial Aerophytic Cyanobacteria in the Canary Island Laurel-Forest (Laurisilva): Discovery of Brasilonema novocanariensis sp. nov. and Rhizonema melkonianarum sp. nov. from the Laurus Phyllosphere

by Nereida M. Rancel-Rodríguez, Christophe Vieira and Marta Sansón

Diversity 2024, 16(10), 625; https://doi.org/10.3390/d16100625 - 10 Oct 2024

Cited by 1 | Viewed by 1188

Abstract

Cyanobacteria, known for their remarkable adaptability, are a diverse group of oxygenic photoautotrophic organisms found in various habitats worldwide. While their presence in aquatic ecosystems has been extensively studied, research on terrestrial and aerophytic cyanobacteria remains relatively limited, particularly concerning their taxonomic diversity [...] Read more.

Cyanobacteria, known for their remarkable adaptability, are a diverse group of oxygenic photoautotrophic organisms found in various habitats worldwide. While their presence in aquatic ecosystems has been extensively studied, research on terrestrial and aerophytic cyanobacteria remains relatively limited, particularly concerning their taxonomic diversity and molecular characteristics. In this study, utilizing morphological and molecular data derived from the 16S rRNA gene, we unveiled and described two new species of filamentous cyanobacteria growing epiphytically on Laurus novocanariensis in the laurel forest (laurisilva) of the Canary Islands. The first species, Brasilonema novocanariensis sp. nov., belongs to a genus of aerophytic and subaerophytic nostocalean cyanobacteria. The second, Rhizonema melkonianarum sp. nov., belongs to the genus Rhizonema. The discovery of a new species of Brasilonema from the Canary Islands related to species initially found on the opposite side of the Atlantic Ocean, and recently in Asia, Europe, and Portugal, raises questions about their dispersal mechanisms, whether through natural means or human-mediated processes, prompting the need for future studies to investigate these propagation patterns. In the case of Rhizonema melkonianarum sp. nov., the transition from adapting to a basidiolichen host to inhabiting the phyllosphere of angiosperms raises ecological and evolutionary adaptation questions regarding cyanobacterial adaptability and their ecological roles in diverse habitats. This research expands our understanding of the diversity and geographic distribution of these terrestrial phyllosphere-associated cyanobacteria. Full article

(This article belongs to the Section Biodiversity Conservation)

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31 pages, 5654 KiB

Open AccessEditor’s ChoiceArticle

The Radiation of Landhoppers (Crustacea, Amphipoda) in New Zealand

by Olivier J.-P. Ball, Alan A. Myers, Stephen R. Pohe and Lara D. Shepherd

Diversity 2024, 16(10), 632; https://doi.org/10.3390/d16100632 - 10 Oct 2024

Viewed by 2021

Abstract

A synopsis of current knowledge of the diversity of the New Zealand landhopper fauna is provided. A combination of morphological and molecular analysis was employed on material from across New Zealand. Thirteen new endemic genera soon to be formally described have been discovered, [...] Read more.

A synopsis of current knowledge of the diversity of the New Zealand landhopper fauna is provided. A combination of morphological and molecular analysis was employed on material from across New Zealand. Thirteen new endemic genera soon to be formally described have been discovered, including four belonging to the widespread families Talitridae and Arcitalitridae. These are families that had not been previously reported from New Zealand. We document the existence of at least 48 new provisional native species. This number far exceeds the 28 species currently described. Some described species are now shown to be species complexes, and a few of these are very diverse with numerous cryptic species. Six changes to the existing taxonomy are proposed. Dallwitzia simularis (Hurley, 1957) is transferred from Makawidae Myers & Lowry, 2020 to Talitridae Rafinesque, 1815; Kellyduncania hauturu (Duncan, 1994) is reinstated as a member of Dana Lowry, 2011; Kellyduncania (Lowry & Myers, 2019) is relegated to a synonym of Dana Lowry, 2011; Kanikania Duncan, 1994 is transferred from Makawidae Myers & Lowry, 2020 to Arcitalitridae Myers & Lowry, 2020; Parorchestia longicornis is transferred to Kanikania Duncan, 1994; Waematau kaitaia (Duncan, 1994) is transferred to Kohuroa Lowry, Myers & Nakano, 2019; and Waematau unuwhao (Duncan, 1994) is transferred to Omaiorchestia Lowry & Myers, 2019. This reduces the number of described New Zealand genera from 17 to 16. Full article

(This article belongs to the Special Issue Diversity and Evolution within the Amphipoda)

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73 pages, 77432 KiB

Open AccessEditor’s ChoiceReview

The Portofino Promontory: 200 Years of History of Marine Biology

by Giorgio Bavestrello, Federico Betti, Carlo Nike Bianchi, Valentina Cappanera, Mariachiara Chiantore, Nicola Corradi, Monica Montefalcone, Mauro Giorgio Mariotti, Carla Morri, Paolo Povero, Giulio Relini, Stefano Schiaparelli and Marzia Bo

Diversity 2024, 16(10), 629; https://doi.org/10.3390/d16100629 - 10 Oct 2024

Viewed by 3168

Abstract

This paper outlines the history of scientific research developed in the Portofino Promontory, located in the centre of the Ligurian Sea. The chronicles span over two centuries, from the late 18th century to the present day. Portofino is now recognised as one of [...] Read more.

This paper outlines the history of scientific research developed in the Portofino Promontory, located in the centre of the Ligurian Sea. The chronicles span over two centuries, from the late 18th century to the present day. Portofino is now recognised as one of the best-known areas in the world regarding marine biological communities and their temporal dynamics, particularly in relation to current climate changes. In addition, since 1999, with the establishment of the Marine Protected Area, significant research related to marine environment conservation has developed in Portofino. The role of the University of Genoa, the Natural History Museum, other important institutions, and the researchers involved in the Portofino area has been outlined. Full article

(This article belongs to the Section Marine Diversity)

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25 pages, 2521 KiB

Open AccessEditor’s ChoiceArticle

A Comparative Analysis of Island vs. Mainland Arthropod Communities in Coastal Grasslands Belonging to Two Distinct Regions: São Miguel Island (Azores) and Mainland Portugal

by Hugo Renato M. G. Calado, Paulo A. V. Borges, Ruben Heleno and António O. Soares

Diversity 2024, 16(10), 624; https://doi.org/10.3390/d16100624 - 9 Oct 2024

Cited by 1 | Viewed by 2047

Abstract

Coastal grasslands host diverse arthropod communities and provide important ecosystem services. Islands, being isolated environments, are expected to have simpler ecosystems than continental areas, with the few successful colonizing species often attaining high densities; however, these patterns are still poorly documented for coastal [...] Read more.

Coastal grasslands host diverse arthropod communities and provide important ecosystem services. Islands, being isolated environments, are expected to have simpler ecosystems than continental areas, with the few successful colonizing species often attaining high densities; however, these patterns are still poorly documented for coastal grassland arthropods. We conducted a comparative study of the biodiversity of arthropod communities in two distinct coastal grassland ecosystems (Portugal mainland and the Azores) with the following objectives: (a) to investigate the arthropod community composition in both locations; (b) to compare the diversity profiles in both locations; (c) to investigate potential density compensation in the island’s arthropod communities. For four months, arthropods were collected on the Island of São Miguel, Setúbal Peninsula, and Sine’s region and subsequently classified into taxonomic groups. With the data collected, Hill Numbers were calculated for each region. We confirmed that the richness on the mainland was higher than in the Azores, and we found some apparent abundance compensation in the Azores. At the same time, we also observed that many species in the Azores are also present in the continental coastal grasslands of mainland Portugal. Full article

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21 pages, 3480 KiB

Open AccessEditor’s ChoiceReview

Patterns of Zoological Diversity in Iran—A Review

by Sajad Noori, Reza Zahiri, Gholam Hosein Yusefi, Mahdi Rajabizadeh, Oliver Hawlitschek, Ehsan Rakhshani, Martin Husemann and Hossein Rajaei

Diversity 2024, 16(10), 621; https://doi.org/10.3390/d16100621 - 8 Oct 2024

Cited by 4 | Viewed by 3262

Abstract

Iran is a country characterized by high biodiversity and complex biogeographic patterns. Its diverse landscape and steep climatic gradients have resulted in significant faunal diversity and high level of endemism. To better understand these patterns, we investigated the historical environmental drivers that have [...] Read more.

Iran is a country characterized by high biodiversity and complex biogeographic patterns. Its diverse landscape and steep climatic gradients have resulted in significant faunal diversity and high level of endemism. To better understand these patterns, we investigated the historical environmental drivers that have shaped Iran’s current geological and climatological conditions, and, consequently, have shaped the current zoological distribution patterns. Furthermore, we provide an overview of the country’s zoological diversity and zoogeography by reviewing published studies on its fauna. We analyzed nearly all available catalogs, updated checklists, and relevant publications, and synthesized them to present a comprehensive overview of Iran’s biodiversity. Our review reports approximately 37,500 animal species for Iran. We also demonstrated that the country serves as a biogeographic transition zone among three zoogeographical realms: the Palearctic, Oriental, and Saharo-Arabian, where distinct faunal elements intersect. This biogeographic complexity has made it challenging to delineate clear zoogeographical zones, leading to varying classifications depending on the taxon. The uplift of mountain ranges, in particular, has played a crucial role in shaping faunal diversity by serving as barriers, corridors, and glacial refugia. These mountains are largely the result of orogeny and plate collisions during the Mesozoic and Cenozoic eras, coupled with the development of the Tethyan Sea and the uplift of several ranges during the Miocene. Despite these insights, our understanding of biodiversity distribution in Iran remains incomplete, even for some well-studied taxa, such as certain vertebrate families and arthropods. We highlight the existing gaps in knowledge regarding zoogeographical patterns and propose approaches to address these gaps, particularly concerning less-studied species and the highly diverse group of insects. Full article

(This article belongs to the Section Animal Diversity)

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18 pages, 2191 KiB

Open AccessEditor’s ChoiceArticle

Meta-Analysis Reveals Behavioral Plasticity, Not Agonistic Behavior, Facilitates Invasion of Brown Anoles (Anolis sagrei) and Replacement of Green Anoles (Anolis carolinensis)

by Maya A. Jackson and Sonny S. Bleicher

Diversity 2024, 16(10), 620; https://doi.org/10.3390/d16100620 - 8 Oct 2024

Viewed by 2452

Abstract

In a meta-analysis, we examined the behavioral portfolio of invasive brown anoles (Anolis sagrei) and native green anoles (Anolis carolinensis) in urban and non-urban environments. We hypothesized that invasive anoles would display more agonistic and bold signals (head bobbing, [...] Read more.

In a meta-analysis, we examined the behavioral portfolio of invasive brown anoles (Anolis sagrei) and native green anoles (Anolis carolinensis) in urban and non-urban environments. We hypothesized that invasive anoles would display more agonistic and bold signals (head bobbing, dewlap extensions, and pushups) than their native-range counterparts and their native competitors. We found that in urban settings, anoles of both species signaled more with dewlap extensions than with head bobs. Brown anoles displayed significantly more in non-urban habitats and their native range compared to urban habitats and invasive ranges. The outcome of our analysis suggests that brown anoles have plastic behavioral portfolios, whereas green anoles have relatively balanced preferences for head bobbing, irrespective of the habitat in which the populations were collected. We attribute the success of the brown anole invasion to the flexible strategy in the face of higher mate competition, higher predation risks, and less resource competition in both urban and invasive ranges. Lastly, we observed publication biases. More studies were conducted with urban and invasive brown anoles and specifically in manipulative mesocosm experimental settings—transplanting populations from native field settings. We show this altered the display rates across all studied signals. Full article

(This article belongs to the Special Issue Ectotherms in a Dynamic Environment: Understanding Patterns of Ecology, Distribution, Evolution, and Threats to Species)

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29 pages, 19422 KiB

Open AccessEditor’s ChoiceArticle

Palaeoenvironmental Analysis of the Southern Part of the Danube–Tisza Interfluve (Hungary): The Northern Loess Wall of Katymár and the Hay Meadows and Loess Banks of Hajós

by Tamás Zsolt Vári, Elemér Pál-Molnár and Pál Sümegi

Diversity 2024, 16(10), 619; https://doi.org/10.3390/d16100619 - 6 Oct 2024

Viewed by 2010

Abstract

This study presents a comprehensive palaeoenvironmental reconstruction of the southern part of the Danube–Tisza interfluve in the Carpathian Basin from the Late Pleistocene to Early Holocene, addressing the region’s response to global climate forcings and local environmental factors based on multi-proxy analyses of [...] Read more.

This study presents a comprehensive palaeoenvironmental reconstruction of the southern part of the Danube–Tisza interfluve in the Carpathian Basin from the Late Pleistocene to Early Holocene, addressing the region’s response to global climate forcings and local environmental factors based on multi-proxy analyses of two key protected areas: the Katymár brickyard and the hay meadows and loess banks of Hajós. By integrating radiocarbon-dated malacological, macrobotanical, pollen, phytolith, geochemical, and sedimentological analyses, it was possible to provide a picture of past climate–ecosystem interactions. The Katymár North sequence provides an extended chronology from Marine Isotope Stage 3 (MIS3, ca. 36,000 cal BP) through the Last Glacial Maximum (LGM) and into the Early Holocene, while the Hajós sequence offers high-resolution data for the LGM–Holocene transition. By the late Ice Age, humidity and surface moisture superseded temperature as primary palaeoecological limiting factors, promoting ecotone-like forest–steppe environments during cooling periods. Full article

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14 pages, 3138 KiB

Open AccessEditor’s ChoiceArticle

Reassessing the Diversity of the Arthropod-Pathogenic Genus Pandora Batko (Entomophthoromycotina; Erynioideae)

by Ann E. Hajek, Andrii P. Gryganskyi, Svetlana Y. Gouli, Tonya D. Bittner, Cheryl F. Sullivan and Bruce L. Parker

Diversity 2024, 16(10), 603; https://doi.org/10.3390/d16100603 - 1 Oct 2024

Cited by 1 | Viewed by 910

Abstract

The fungal order Entomophthorales occurs worldwide, with most species infecting arthropods as pathogens. Species in this order can cause epizootics and change the behavior of infected hosts. Molecular data are available only for 20% of the known species, and distributions of species are [...] Read more.

The fungal order Entomophthorales occurs worldwide, with most species infecting arthropods as pathogens. Species in this order can cause epizootics and change the behavior of infected hosts. Molecular data are available only for 20% of the known species, and distributions of species are seldom summarized. Significant diversity of hosts, poor molecular data availability, and poor resolution of the phylogenetic relationships within this fungal order suggest that the diversity of these fungi is not sufficiently described. The subfamily Erynioideae includes 111 arthropod pathogens, divided among six genera, with the genus Pandora being one of the most diverse genera. Sequences of 18S, 28S, and ITS for two species are used to place these Pandora species in a phylogenic tree of the subfamily; this tree also supports our synonymy of the genus Furia with Pandora. Among the two species specifically covered in this paper, Pandora gloeospora was observed during epizootics occurring in mushroom flies (Diptera: Sciaridae) on Agaricus bisporus cultures in Pennsylvania, Delaware, and Maryland (US) mushroom farms and also in Florida on Pleurotus sp. Outside the US, P. gloeospora was found infecting several Nematocera (Diptera) in Europe (France) and Asia (China). Pandora sylvestris n. sp. was collected during epizootics occurring in larvae of hickory tussock moths, Lophocampa caryae (Lepidoptera: Erebidae), in hardwood forests in Michigan and Vermont. Full article

(This article belongs to the Section Phylogeny and Evolution)

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15 pages, 4560 KiB

Open AccessEditor’s ChoiceArticle

Influence of Biofloc Technology and Continuous Flow Systems on Aquatic Microbiota and Water Quality in Japanese Eel Aquaculture

by Hyunjun Choi, Jun Seong Park, Ju-Ae Hwang, Shin-Kwon Kim, Yehyeon Cha and Seung-Yoon Oh

Diversity 2024, 16(10), 601; https://doi.org/10.3390/d16100601 - 1 Oct 2024

Cited by 2 | Viewed by 1407

Abstract

Biofloc technology (BFT) systems heavily rely on microbiota to mitigate ammonia toxicity and manage essential nutrient cycling. Understanding the diversity and functional role of microbiota within BFT-applied aquaculture systems is crucial for ensuring sustainable operations. Though some studies exist on BFT microbiota, research [...] Read more.

Biofloc technology (BFT) systems heavily rely on microbiota to mitigate ammonia toxicity and manage essential nutrient cycling. Understanding the diversity and functional role of microbiota within BFT-applied aquaculture systems is crucial for ensuring sustainable operations. Though some studies exist on BFT microbiota, research on microbial differences in Japanese eel aquaculture is still limited, hindering the wider application of BFT systems. In this study, we analyzed the characteristics of water quality factors and microbiota in Japanese eel (Anguilla japonica) breeding water, applying the BFT system. Using a metabarcoding approach, the diversity and community structure of aquatic microbiota were compared between BFT and continuous flow (CF) systems. The pH was significantly higher in CF water, while total ammonia nitrogen (TAN) and nitrite (NO₂⁻-N) was higher in BFT water. Alpha diversity was significantly higher in BFT compared to CF systems, and it was correlated significantly with pH and TAN. In both BFT and CF water, the phyla Proteobacteria and Bacteroidota were found to be the most abundant. In the BFT water, a diverse array of bacterial taxa, including BFT-specific clades, were consistently present, while the microbiota in CF water was more variable and contained fewer specific taxa. In addition, bacterial functions related to nitrate reduction, sulfur compound oxidation, and chitinolysis were significantly more abundant in BFT than in CF systems. These findings highlight differences in water quality and microbiota between aquaculture systems, which can inform future research on the use of BFT for sustainable fish farming. Full article

(This article belongs to the Section Microbial Diversity and Culture Collections)

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35 pages, 6301 KiB

Open AccessEditor’s ChoiceArticle

Multi-Biomarker Analysis Uncovers High Spatio-Temporal Stability of a Subarctic Rhodolith (Lithothamnion glaciale) Bed Food Web

by Sean Hacker Teper, Christopher C. Parrish and Patrick Gagnon

Diversity 2024, 16(10), 597; https://doi.org/10.3390/d16100597 - 27 Sep 2024

Cited by 1 | Viewed by 2176

Abstract

We used lipid, fatty acid, and stable isotope analyses to investigate variation, over nine months, in the trophodynamics of 10 dominant cryptofaunal, macroalgal/algal, and environmental components from two sites within a rhodolith (Lithothamnion glaciale) bed in southeastern Newfoundland (Canada). There was [...] Read more.

We used lipid, fatty acid, and stable isotope analyses to investigate variation, over nine months, in the trophodynamics of 10 dominant cryptofaunal, macroalgal/algal, and environmental components from two sites within a rhodolith (Lithothamnion glaciale) bed in southeastern Newfoundland (Canada). There was an overall shift from a diatom-based food web following the spring phytoplankton bloom to a kelp/algae-based food web during fall, accompanied by preferred use of EPA (20:5ω3) over DHA (22:6ω3) in most cryptofauna. The food web contained three trophic levels that encompassed: (1) direct feeding relationships from primary producers (e.g., rhodoliths, macroalgae) to second-order consumers (e.g., sea stars, polychaetes); (2) trophic subsidy from within and outside the rhodolith bed via settlement, resuspension, and consumption of macroalgal fragments and other detrital organic matter; and (3) strong pelagic/benthic coupling. Riverine input did not affect cryptofaunal diets, as shown by the lack of terrestrial biomarkers at the study site nearest to the riverine input, and there were minor differences in trophodynamics between both study sites. The present study’s findings, applicable to relatively broad spatial and temporal domains, as well as those of complementary studies of the same rhodolith bed, uncover high spatio-temporal stability of the rhodolith bed framework and of resident cryptofaunal abundance, diversity, and trophodynamics. Full article

(This article belongs to the Special Issue Marine Nearshore Biodiversity—2nd Edition)

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26 pages, 10119 KiB

Open AccessEditor’s ChoiceArticle

An Ecomorphological Description of Amblyraja radiata (Rajiformes: Rajidae) in Waters of Eastern Canada

by David W. Kulka, Carolyn M. Miri, Sheila Atchison and Mark R. Simpson

Diversity 2024, 16(10), 595; https://doi.org/10.3390/d16100595 - 26 Sep 2024

Viewed by 1735

Abstract

We examine the distribution, habitat association, morphometrics, meristics, and reproductive attributes of Amblyraja radiata over much of its Canadian range, Grand Banks to Arctic waters. It is distributed widely on the shelf and upper slope between 30 and 1288 m, reaching highest density [...] Read more.

We examine the distribution, habitat association, morphometrics, meristics, and reproductive attributes of Amblyraja radiata over much of its Canadian range, Grand Banks to Arctic waters. It is distributed widely on the shelf and upper slope between 30 and 1288 m, reaching highest density in 100–400 m and occupying most available temperatures, between −1.0 and 8.8 °C, but concentrating in 1.6–3.5 °C. The maximum (and average) size decreases with increasing latitude in a continuum from 102 cm (55 cm) in the south, to 45 cm (20 cm) in the north. The proportion of mature fish increases with depth (40% at 0–50 m to 80% at 1150–1200 m) and temperature (35% at <0 °C to 55% at 5+ °C). The size at maturity decreases south to north; size at onset of maturity in males—43 (south) to 19 (north) cm, in females—49 to 23 cm; length at 50% maturity in males—74 to 44 cm, in females—66 to 40 cm. A. radiata maturity is also reflected in the rapid increase in the size of secondary sexual characteristics. Some meristics were consistent over the entire study area (spines near the spiracles and shoulders) while others varied with latitude (teeth rows, midline spines, spines near the eyes, % dorsal fins joined, spines between dorsal fins) or by fish length/maturity; the tail length/total length as a proportion of total length decreased during Stage 1 then increased at onset of maturity. Full article

(This article belongs to the Special Issue Recent Advances in Ecology, Evolution and Biodiversity of the Chondrichthyan Fauna)

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22 pages, 2104 KiB

Open AccessEditor’s ChoiceArticle

Burrowing Owls Require Mutualist Species and Ample Interior Habitat Space

by K. Shawn Smallwood and Michael L. Morrison

Diversity 2024, 16(9), 590; https://doi.org/10.3390/d16090590 - 19 Sep 2024

Viewed by 1423

Abstract

Mitigating habitat loss of western burrowing owls (Athene cunicularia hypugaea) often involves relocation from California ground squirrel (Otospermophilus beecheyi) burrows to offsite nest boxes. Naval Air Station Lemoore (NASL), Kings and Fresno counties, California, initiated this approach to displace [...] Read more.

Mitigating habitat loss of western burrowing owls (Athene cunicularia hypugaea) often involves relocation from California ground squirrel (Otospermophilus beecheyi) burrows to offsite nest boxes. Naval Air Station Lemoore (NASL), Kings and Fresno counties, California, initiated this approach to displace a regionally important population from airfield grasslands. We examined monitoring data of burrowing owls and fossorial mammals at NASL to assess mitigation options. Occupied nests increased by 33 (61%), with 47 nest box installations in 1997–2001, peaked at 87 in 1999, then declined by 50 through 2013. Although ≥13 nest boxes were occupied in 2000, none were occupied in 2003–2013. Within a 43.1 ha isolated grassland monitored for 13 years, nest site reuse in ground squirrel burrows averaged only 17% between any 2 consecutive years. Compared to the average density across grassland study areas, ground squirrel burrow systems/ha numbered 43% higher within 60 m of occupied nests and non-breeding-season burrows. Vegetation clearing to restore kangaroo rat (Dipodomys n. nitratoides) habitat preceded a 7.4-fold increase in ground squirrel burrow systems and a 4-fold increase in occupied nests, but drought-induced extirpation of ground squirrels eliminated occupied nests from the 43.1 ha grassland study area. Ground cover near occupied nests averaged 58% of the mean vegetation height and 67% of the mean percentage of bare ground in the field. Both nest sites and non-breeding-season burrows occurred >60 m interior to field edges 1.4 times more than expected. Non-breeding-season burrows averaged 328 m from same-year nest sites, and only 7% of non-breeding-season burrows were also used as nest sites. Mitigating habitat loss should be made more effective by fostering natural burrow construction by fossorial mammals on patches of short-stature vegetation that is sufficiently expansive to support breeding colonies of ≥12 pairs averaging ≥60 m from the field’s edge and a separation between non-breeding-season burrows and nest burrows minimally equal to mean nearest-neighbor distances among nests. Full article

(This article belongs to the Section Biodiversity Loss & Dynamics)

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22 pages, 10641 KiB

Open AccessEditor’s ChoiceArticle

Systematic of a Massively Constructed Specimen of Iguanodon galvensis (Ornithopoda, Iguanodontidae) from the Early Barremian (Early Cretaceous) of Eastern Spain

by Josué García-Cobeña, Francisco J. Verdú and Alberto Cobos

Diversity 2024, 16(9), 586; https://doi.org/10.3390/d16090586 - 17 Sep 2024

Cited by 2 | Viewed by 4409

Abstract

Styracosternan ornithopods are plenty abundant in the Lower Cretaceous fossil record of Europe. In particular, Iguanodon, the second genus of dinosaurs described worldwide, has been found in UK, Belgium, France, Germany, and Spain, evidencing a wide geographical distribution. Currently, the genus Iguanodon [...] Read more.

Styracosternan ornithopods are plenty abundant in the Lower Cretaceous fossil record of Europe. In particular, Iguanodon, the second genus of dinosaurs described worldwide, has been found in UK, Belgium, France, Germany, and Spain, evidencing a wide geographical distribution. Currently, the genus Iguanodon comprises two species, the type species I. bernissartensis from the late Barremian–Aptian of Europe and I. galvensis from the early Barremian of Teruel, Spain. The latter species is well known mainly from perinate and juvenile specimens. Here, axial and appendicular fossils of an adult, large and massively constructed ornithopod from the lower Barremian (Lower Cretaceous) Camarillas Formation of Galve (province of Teruel, Spain) are described. Fossil dimensions and some osteological evidence reveal that the specimen was a large (roughly 10 m long) ornithopod. An autapomorphic feature in the ischium and other characters allow us to ascribe this specimen to I. galvensis. In addition, postcranial co-ossification and fusion of the neurocentral suture indicate that the specimen was skeletally mature. Part of the material studied here was unknown in adults of I. galvensis, providing a better knowledge of the axial and appendicular region of this species. Full article

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10 pages, 1826 KiB

Open AccessEditor’s ChoiceArticle

An Initial Genetic Assessment of the Emblematic Pumas of the Torres del Paine UNESCO Biosphere Reserve

by L. Mark Elbroch, Byron V. Weckworth, Kristine Pilgrim, Omar Ohrens, Nicolás Lagos, Stephanny Arroyo-Arce, Mauricio Montt, Dania Goic and Michael K. Schwartz

Diversity 2024, 16(9), 581; https://doi.org/10.3390/d16090581 - 13 Sep 2024

Viewed by 1638

Abstract

Physical and genetic isolation are recognized as significant threats to wildlife, especially in large carnivores inhabiting fragmented landscapes. We conducted an initial genetic assessment of pumas (Puma concolor) using 19 microsatellite loci for the emblematic puma population in the Torres del [...] Read more.

Physical and genetic isolation are recognized as significant threats to wildlife, especially in large carnivores inhabiting fragmented landscapes. We conducted an initial genetic assessment of pumas (Puma concolor) using 19 microsatellite loci for the emblematic puma population in the Torres del Paine UNESCO Biosphere Reserve in southernmost Chile, which exhibits some distinctive phenology that some local people speculate may be due to isolation and inbreeding depression. We extracted DNA from 385 scats collected in the field, of which 96 were identified as puma, representing 20 unique individuals. Torres del Paine pumas exhibited an H_o (0.51) only slightly lower than H_e (0.53), with 2 of the 19 loci significantly out of Hardy–Weinberg Equilibrium. Tests for a recent bottleneck of the population were not significant. The small sample size of individuals notwithstanding, these results seemingly do not support high levels of inbreeding. We also identified individual pumas in the field and assessed them for observable cowlicks (twirls of fur on their backs), a trait some have associated with genetic inbreeding depression in other puma populations. A total of 7 of 39 pumas exhibited cowlicks, consistent with geographic patterns of cowlicks within the species. Our tests exploring population structure among local pumas provided the most support for a single-population cluster, but we explored secondary structures as well, given its conservation implications. We encourage additional sampling in the region to further explore population structure and connectivity and determine the conservation status of the region’s pumas to guide the development of best strategies to ensure their persistence. Full article

(This article belongs to the Section Animal Diversity)

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26 pages, 10142 KiB

Open AccessEditor’s ChoiceArticle

Dispersal Ecology of Golden Eagles (Aquila chrysaetos) in Northern Greece: Onset, Ranging, Temporary and Territorial Settlement

by Lavrentis Sidiropoulos, D. Philip Whitfield, Konstantinos Poirazidis, Elisabeth Navarrete, Dimitris P. Vasilakis, Anastasios Bounas, Elzbieta Kret and Vassiliki Kati

Diversity 2024, 16(9), 580; https://doi.org/10.3390/d16090580 - 13 Sep 2024

Viewed by 2992

Abstract

Natal dispersal is a crucial period for raptors with serious implications for individuals’ survival and population demography. In this study we analyzed data from 18 GPS-tracked golden eagles in order to describe their dispersal ecology in northern Greece, where the species feeds mostly [...] Read more.

Natal dispersal is a crucial period for raptors with serious implications for individuals’ survival and population demography. In this study we analyzed data from 18 GPS-tracked golden eagles in order to describe their dispersal ecology in northern Greece, where the species feeds mostly on tortoises. Young eagles in our population dispersed at 176 days post fledging, spent their first year of independence relatively close (40–60 kms) to their natal ranges and exhibited a variable temporary settlement behavior. Overall dispersal range sizes did not differ seasonally, but temporary settlement area range sizes were significantly larger in winter. Three eagles survived to territorial settlement and occupied ranges 20–60 kms from their natal areas. The application and refinement of the Scottish GET dispersal ranging model suggested that eagles used areas that had higher topographical relief and lower canopy cover during their natal dispersal. Habitat heterogeneity seems to also be influential during temporary settlement. Our study is the first to provide both such insights for golden eagles in southern eastern Europe and a method for delineating temporary settlement areas for the species. Our findings can be explained in terms of food and habitat availability. We highlight the importance of conserving heterogeneous open areas of complex topography and applying proactive management measures within temporary settlement areas for our population’s conservation. Full article

(This article belongs to the Special Issue Conservation and Ecology of Raptors—2nd Edition)

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27 pages, 9128 KiB

Open AccessEditor’s ChoiceArticle

Revalidation of the Arboreal Asian Snake Genera Gonyophis Boulenger, 1891; Rhynchophis Mocquard, 1897; and Rhadinophis Vogt, 1922, with Description of a New Genus and Tribe (Squamata: Serpentes: Colubridae)

by Van Wallach, Rune Midtgaard and Emma Hsiao

Diversity 2024, 16(9), 576; https://doi.org/10.3390/d16090576 - 13 Sep 2024

Viewed by 2383

Abstract

Based on the latest molecular phylogenies of Gonyosoma sensu lato, which recovered five clades with robust support, we utilize morphological characters to demonstrate the distinctiveness of each clade, resulting in the resurrection of three genera (Gonyophis Boulenger, 1891; Rhynchophis Mocquard, 1897; and [...] Read more.

Based on the latest molecular phylogenies of Gonyosoma sensu lato, which recovered five clades with robust support, we utilize morphological characters to demonstrate the distinctiveness of each clade, resulting in the resurrection of three genera (Gonyophis Boulenger, 1891; Rhynchophis Mocquard, 1897; and Rhadinophis Vogt, 1922) and a proposal of a new genus and a new tribe. A synopsis of the group, with descriptions and diagnoses, is provided for the five genera and eight species in addition to distribution maps and illustrations of the head of each taxon. An artificial key to the species and genera in the new tribe is presented in addition to the estimated origin times for each clade. Full article

(This article belongs to the Collection Feature Papers in Animal Diversity)

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37 pages, 18007 KiB

Open AccessEditor’s ChoiceArticle

The Problematic Genus Liodesmus Wagner and a New Genus of Caturoidea (Halecomorphi, Neopterygii) from the Upper Jurassic Solnhofen-Archipelago

by Martin Ebert and Adriana López-Arbarello

Diversity 2024, 16(9), 568; https://doi.org/10.3390/d16090568 - 12 Sep 2024

Viewed by 4836

Abstract

Our revision of the type material of Liodesmus led to significant adjustments in our understanding of the taxon when the original type specimen of Pholidophorus gracilis, which was later determined as the type species of the genus Liodesmus, was recently found in [...] Read more.

Our revision of the type material of Liodesmus led to significant adjustments in our understanding of the taxon when the original type specimen of Pholidophorus gracilis, which was later determined as the type species of the genus Liodesmus, was recently found in the collection in Berlin. It had been confused with a specimen that was described as the type specimen of the species Liodesmus gracilis in the collection in Munich. Moreover, the Munich specimen was mistakenly considered by all authors who have studied Liodesmus as a type specimen for the genus Liodesmus. However, the original holotype of Pholidophorus gracilis and this Munich specimen of Liodesmus gracilis belong to two different families. Caturus brevicostatus, whose holotype was recently found in Berlin as well, is described in detail here for the first time. Liodesmus sprattiformis is now described under the new genus Nasrinsotoudehichthys outside Caturidae but within the Caturoidea. Since all specimens that have been described so far under Liodesmus either belong to other, previously described genera, belong to new genera clearly outside the description of the genus Liodesmus, or are nomina dubia (including the type species Pholidophorus gracilis), the genus Liodesmus and the Liodesmidae are nomina dubia as well. Full article

(This article belongs to the Special Issue Diversity, Biogeography and Evolution of Actinopterygians)

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18 pages, 1028 KiB

Open AccessEditor’s ChoiceArticle

Structure of Reef Fish Families (Butterflyfishes and Angelfishes) at Isolated Oceanic Reefs in the Indian Ocean: Christmas Island and the Cocos (Keeling) Islands

by Jean-Paul A. Hobbs and Maya Srinivasan

Diversity 2024, 16(9), 569; https://doi.org/10.3390/d16090569 - 12 Sep 2024

Cited by 3 | Viewed by 1109

Abstract

There has been substantial research on the factors that structure reef fish assemblages, but this has mostly focused on nearshore or continental reefs. This study examines patterns of abundance and species composition for two iconic groups of coral reef fishes, angelfishes (family Pomacanthidae) [...] Read more.

There has been substantial research on the factors that structure reef fish assemblages, but this has mostly focused on nearshore or continental reefs. This study examines patterns of abundance and species composition for two iconic groups of coral reef fishes, angelfishes (family Pomacanthidae) and butterflyfishes (family Chaetodontidae) at two isolated, oceanic reefs in the Indian Ocean: Christmas Island and the Cocos (Keeling) Islands. Six explanatory variables were investigated to determine whether large-scale physical factors are more important than fine-scale biotic factors in structuring reef fish communities on oceanic islands. For angelfishes, depth was the factor that most explained patterns in abundance (explaining 46.5% of the variation), species richness (44.8%) and composition (15.3%), with both abundance and species richness being greater at 20 m than at 5 m. Differences in species composition were greater between depths than between islands. For butterflyfishes, variation in abundance and species richness was best explained by the difference in aspect or exposure among sites, though abundance and composition also differed significantly between Christmas Island and the Cocos (Keeling) Islands. Large-scale variation in the structure of these reef fish assemblages could not be explained based on differences in habitat diversity, or coral and algal cover. This study indicates that large-scale physical factors (island location, exposure, depth) are more important than fine-scale biotic factors in structuring reef fish assemblages on oceanic islands. Full article

(This article belongs to the Special Issue Diversity in 2024)

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18 pages, 9810 KiB

Open AccessEditor’s ChoiceArticle

Biodiversity and Possible Bio-Indicators of Mediterranean Temporary Ponds in Southern Apulia, Italy

by Leonardo Beccarisi, Vincenzo Zuccarello, Rita Accogli and Genuario Belmonte

Diversity 2024, 16(9), 559; https://doi.org/10.3390/d16090559 - 7 Sep 2024

Viewed by 1757

Abstract

Mediterranean Temporary Ponds (MTPs) represent a priority habitat according to Directive 92/43/EEC (Natura 2000 code: 3170*). These are very shallow water habitats only seasonally flooded, with a flora mainly composed of Mediterranean therophytic and geophytic species. Its extreme seasonality and small size make [...] Read more.

Mediterranean Temporary Ponds (MTPs) represent a priority habitat according to Directive 92/43/EEC (Natura 2000 code: 3170*). These are very shallow water habitats only seasonally flooded, with a flora mainly composed of Mediterranean therophytic and geophytic species. Its extreme seasonality and small size make this habitat highly vulnerable and hard to manage. In recent Italian monitoring campaigns, the conservation status of MTP 3170* was considered inadequate. In Apulia, where the habitat is considered as “the most vulnerable type”, 73 sites were censused, with a total coverage of about 10,000 m². The present work refers to the monitoring for three years of a total of 16 habitat 3170* sites, with the aim of better describing faunal indicator species for this priority habitat. A total of 158 taxa of flora and 103 of fauna were identified from 54 floristic and 44 faunistic samplings in total, with a robust updating of the listed biodiversity. For the first time a group of faunal species is proposed as an indicator of the habitat MTP 3170*. The conservation status, assessed on the basis of structural and functional criteria, gave a satisfactory status for seven sites and an unsatisfactory one (variously rated as inadequate or bad) for nine. Full article

(This article belongs to the Special Issue Diversity, Ecology and Conservation of Zooplankton)

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42 pages, 47060 KiB

Open AccessEditor’s ChoiceArticle

A Molecular-Informed Species Inventory of the Order Ceramiales (Rhodophyta) in the Narragansett Bay Area (Rhode Island and Massachusetts), USA

by Thomas Irvine, Brian Wysor and Alicia Beauvais

Diversity 2024, 16(9), 554; https://doi.org/10.3390/d16090554 - 5 Sep 2024

Viewed by 2101

Abstract

Narragansett Bay is an estuarine system in the western North Atlantic Ocean that harbors a diverse marine flora, providing structure, habitat, and food for native biodiversity. This area has been the center of numerous environmental, biological, ecological, and oceanographic studies; however, marine macroalgae [...] Read more.

Narragansett Bay is an estuarine system in the western North Atlantic Ocean that harbors a diverse marine flora, providing structure, habitat, and food for native biodiversity. This area has been the center of numerous environmental, biological, ecological, and oceanographic studies; however, marine macroalgae have not been extensively examined using modern molecular methods. Here, we document the biodiversity of the red algal order Ceramiales based on DNA sequence comparisons of the 3′ end of the RuBisCo large subunit (rbcL-3P) and the universal plastid amplicon (UPA). Thirty-seven distinct species of this order were identified and validated with molecular data, including five new species reports and at least one new report of an introduced species, Antithamnionella spirographidis, in the vicinity of Narraganset Bay. Novel sequence data were generated for numerous species, and it was discovered that the UPA marker, which has been less frequently used in red algal floristics, revealed an identical inventory of ceramialean algae as the rbcL-3P marker. Thus, the shorter length of the UPA marker holds promise for DNA metabarcoding studies that seek to elucidate biodiversity across algal phyla. Full article

(This article belongs to the Special Issue DNA Barcodes for Evolution and Biodiversity—2nd Edition)

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53 pages, 7160 KiB

Open AccessEditor’s ChoiceArticle

New Evidence of the Feeding Behaviors of Coronodon and the Origin of Filter Feeding in Mysticetes (Mammalia: Cetacea) Revisited

by Jonathan H. Geisler, Brian L. Beatty and Robert W. Boessenecker

Diversity 2024, 16(9), 549; https://doi.org/10.3390/d16090549 - 5 Sep 2024

Viewed by 3411

Abstract

Coronodon includes species of basal toothed mysticetes that were initially interpreted as engaging in raptorial feeding and dental filtration. Here, the feeding of this extinct genus is revisited based on recently described specimens and species. Associations between tooth position and types of dental [...] Read more.

Coronodon includes species of basal toothed mysticetes that were initially interpreted as engaging in raptorial feeding and dental filtration. Here, the feeding of this extinct genus is revisited based on recently described specimens and species. Associations between tooth position and types of dental wear were tested, and evidence for feeding behaviors was tabulated using scores from 14 craniodental characters, each mapped onto five alternate phylogenetic hypotheses. Individual character states were interpreted as being supportive, neutral, or contradictory evidence to raptorial feeding, suction feeding, baleen filtration, or dental filtration. Wear in Coronodon was found to be significantly more concentrated on mesial teeth, mesial cusps, higher cusps, and upper teeth. Upper teeth also had mesial cusps more worn than distal cusps, inconsistent with predictions of the dental filtration hypothesis. Wear in notches was correlated with wear on neighboring cusps, and side wear was concentrated on occlusal sides, suggesting both were caused by raptorial feeding. These observations raise the possibility that raptorial feeding was the primary, and maybe even the only, mode of feeding for Coronodon. The feeding scores of reconstructed ancestors leading to crown mysticetes typically display a stepwise decrease in raptorial feeding, a stepwise increase in baleen filtration, and, occasionally, an intermediate but weakly supported stage of dental filtration. For most toothed mysticetes, there is little evidence for or against suction feeding. The method we have developed for studying the origin of baleen can be expanded and allows for multiple hypotheses to be tested without undue emphasis on any particular taxon or set of characters. Full article

(This article belongs to the Special Issue Evolution of Crown Cetacea)

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16 pages, 3588 KiB

Open AccessEditor’s ChoiceArticle

First Virtual Reconstruction of a Mosasaurid Brain Endocast: Description and Comparison of the Endocast of Tethysaurus nopcsai with Those of Extant Squamates

by Rémi Allemand, Michael J. Polcyn, Alexandra Houssaye, Peggy Vincent, Camilo López-Aguirre and Nathalie Bardet

Diversity 2024, 16(9), 548; https://doi.org/10.3390/d16090548 - 5 Sep 2024

Viewed by 2480

Abstract

Paleoneurological studies of mosasaurids are few and limited to old partial reconstructions made from latex casts on Platecarpus and Clidastes. Here, the brain endocasts of three specimens of the early mosasaurid Tethysaurus nopcsai from the Turonian of Morocco are reconstructed for the [...] Read more.

Paleoneurological studies of mosasaurids are few and limited to old partial reconstructions made from latex casts on Platecarpus and Clidastes. Here, the brain endocasts of three specimens of the early mosasaurid Tethysaurus nopcsai from the Turonian of Morocco are reconstructed for the first time by using micro-computed tomography. Comparisons between Tethysaurus and the later Platecarpus and Clidastes show that distinct endocranial organizations have occurred within the clade through time, including differences in the flexure of the endocast and the size of the parietal eye. The physiological consequences of such variability remain unclear and further investigations are required to better interpret these variations. In addition, the endocast of Tethysaurus was compared to those of extant anguimorphs, iguanians, and snakes, using landmark-based geometric morphometrics. The results revealed that Tethysaurus exhibits a unique combination of endocranial features compared to extant toxicoferans. Contrary to previous statements, we find no strong resemblance in endocast morphology between Tethysaurus and varanids. Rather, the endocast of Tethysaurus shows some morphological similarities with each of the clades of anguimorphs, iguanians, and snakes. In this context, while a notable phylogenetic signal is observed in the variability of squamate endocasts, it is premature to establish any phylogenetic affinities between mosasaurids and extant squamates based solely on endocast morphologies. Full article

(This article belongs to the Special Issue Making New Out of the Old: Recent Biological Advances on Mesozoic Marine Reptiles)

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24 pages, 34155 KiB

Open AccessEditor’s ChoiceArticle

Anatomy and Relationships of a New Gray Whale from the Pliocene of Piedmont, Northwestern Italy

by Michelangelo Bisconti, Piero Damarco, Lorenza Marengo, Mattia Macagno, Riccardo Daniello, Marco Pavia and Giorgio Carnevale

Diversity 2024, 16(9), 547; https://doi.org/10.3390/d16090547 - 5 Sep 2024

Cited by 2 | Viewed by 1790

Abstract

A new fossil gray whale genus and species, Glaucobalaena inopinata, is established based on craniomandibular remains from the Pliocene Sabbie d’Asti Formation, Piedmont, northwestern Italy. The holotype (MGPT-PU 19512) consists of two cranial fragments corresponding to the posterolateral corners of the skull, [...] Read more.

A new fossil gray whale genus and species, Glaucobalaena inopinata, is established based on craniomandibular remains from the Pliocene Sabbie d’Asti Formation, Piedmont, northwestern Italy. The holotype (MGPT-PU 19512) consists of two cranial fragments corresponding to the posterolateral corners of the skull, including both partial periotics, and in the posterior portion of the right mandibular ramus preserving the condyle and angular process. The new taxon is characterized by gray whale (eschrichtiid) synapomorphies in the posterior portion of the mandible (dorsally raised mandibular condyle with articular surface faced dorsoposteriorly, well-developed and robust angular process of the mandible) and in the earbone (massive transverse elongation of the pars cochlearis, indistinct flange of the ventrolateral tuberosity, and triangular and short anterior process of the periotic). A CT scan of the cranial fragments allowed us to reconstruct tridimensional renderings of the periotic, revealing the dorsal morphology of this bone. A phylogenetic analysis confirmed the inclusion of Glaucobalaena inopinata within Eschrichtiidae (the family to whom gray whales are included) and showed that it is monophyletic with Gricetoides aurorae; our phylogenetic results show that Eschrichtioides gastaldii is the sister group of the genus Eschrichtius. Our work lends further support to the idea that Eschrichtiidae is a separate family of baleen whales, characterized by specialized ecomorphological characters evident in both skull and mandibular architecture. Full article

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16 pages, 1506 KiB

Open AccessEditor’s ChoiceArticle

Temporal Changes in Bank Vole Populations Indicate Species Decline

by Linas Balčiauskas, Marius Jasiulionis, Vitalijus Stirkė and Laima Balčiauskienė

Diversity 2024, 16(9), 546; https://doi.org/10.3390/d16090546 - 4 Sep 2024

Cited by 2 | Viewed by 1014

Abstract

Because of their wide distribution, short life cycle, rapid reproduction, and sensitivity to the environment, rodents can indicate changes in habitat quality and climate variables. Long-term studies are needed to verify these changes and assumptions about their causes. We analyzed small mammal trapping [...] Read more.

Because of their wide distribution, short life cycle, rapid reproduction, and sensitivity to the environment, rodents can indicate changes in habitat quality and climate variables. Long-term studies are needed to verify these changes and assumptions about their causes. We analyzed small mammal trapping data in Lithuania, covering the period 1975–2023, with 1821 trapping sites and 57,426 small mammal individuals, with a focus on the bank vole (Clethrionomys glareolus). The aim of this study was to assess temporal changes in the relative abundance and proportion of this species in small mammal communities in relation to their habitats. With 21,736 captured individuals, C. glareolus was a dominant species in the country; its proportion in general was 37.9%, with 60.0% in forests. Open habitats, meadows and agricultural land were characterized by the lowest species proportions. Our main findings were the confirmation of decreasing abundances and proportions of C. glareolus since the 1990s, the absence of cyclical fluctuations in the relative abundances of the species in general and in forest habitats, and the introduction of a south–north cline in species proportions. The status of this temperate and boreal forest species is subject to change, with implications for the diversity of the mid-latitude small mammal community. Full article

(This article belongs to the Special Issue Diversity in 2024)

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20 pages, 6172 KiB

Open AccessEditor’s ChoiceArticle

Bacterial Community Structure and Patterns of Diversity in the Sediments of Mountain Rock Basins from a National Park

by Amaya de Cos-Gandoy, Andrea Serrano-Bellón, María Macías-Daza, Blanca Pérez-Uz, Richard A. J. Williams, Abel Sanchez-Jimenez and Mercedes Martín-Cereceda

Diversity 2024, 16(9), 544; https://doi.org/10.3390/d16090544 - 4 Sep 2024

Cited by 1 | Viewed by 3867

Abstract

Bacterial microbiomes influence global carbon and nutrient cycling as the environment changes. Rain-fed rock basins are ephemeral aquatic systems, potentially subject to extreme environmental stress, that can host a wide variety of biological communities, including bacteria. However, bacterial communities are barely described in [...] Read more.

Bacterial microbiomes influence global carbon and nutrient cycling as the environment changes. Rain-fed rock basins are ephemeral aquatic systems, potentially subject to extreme environmental stress, that can host a wide variety of biological communities, including bacteria. However, bacterial communities are barely described in these habitats. Here we provide a detailed description on the occurrence, diversity and distribution patterns of the bacterial communities within and between rain-fed granite mountain rock basins located in the Sierra de Guadarrama National Park, Spain, using high-throughput sequencing of 16S RNA. We recovered a highly diverse community consisting of 3174 operational taxonomic units (OTUs) belonging to 32 phyla. In total, 50% of OTUs were shared among basins and 6–10% were basin-exclusive OTUs, suggesting a robust global bacterial metacommunity colonizes the basins. The existence of 6% replicate-exclusive OTUs and the fact that at least four replicates were required to catalogue 90% of the basin bacterial community emphasized the heterogeneity of these habitats. Both environmental filtering and random dispersal are likely to be involved in the arrangement of the bacterial communities. The taxa identified in this study are versatile in metabolism, and some have biotechnological potential. The taxonomic affiliation of many of the OTUs found suggests that rain-fed rock basins could be a resource for mining novel bacterial biocompounds. Full article

(This article belongs to the Special Issue Montane Ecosystems and Diversity)

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40 pages, 18114 KiB

Open AccessEditor’s ChoiceArticle

Arachnid Assemblage Composition Diverge between South- and North-Facing Slopes in a Levantine Microgeographic Site

by Meir Finkel, Amit Ben-Asher, Gur Shmula, Igor Armiach Steinpress, Zeana Ganem, Rami Hammouri, Erika Garcia, Tamás Szűts and Efrat Gavish-Regev

Diversity 2024, 16(9), 540; https://doi.org/10.3390/d16090540 - 3 Sep 2024

Cited by 1 | Viewed by 1757

Abstract

Local microgeographic sites subdivided by sharp ecological and climatic contrasts are important platforms for measuring biodiversity patterns and inferring the possible effect of climatic and ecological variables on species distributions and habitat use. Here, we report results from 24 months (September 2019–August 2021) [...] Read more.

Local microgeographic sites subdivided by sharp ecological and climatic contrasts are important platforms for measuring biodiversity patterns and inferring the possible effect of climatic and ecological variables on species distributions and habitat use. Here, we report results from 24 months (September 2019–August 2021) of continuous pitfall trapping collection in Lower Nahal Keziv, Western Upper Galilee, Israel (“Evolution Canyon” II (hereafter—EC II)). This site receives an average annual rainfall of 784 mm and contains two slopes that differ markedly by solar radiation and plant formation. The first is the south-facing slope (SFS), which is characterized as a semiarid garrigue and open grassland. The second is the contrasting north-facing slope (NFS), which is characterized by a more humid East Mediterranean forest. The slopes are separated by a narrow valley bottom (VB). Analysis of ca. 1750 arachnid specimens, collected from 70 pitfall traps along the slopes and valley, indicates significantly different arachnid assemblages between the NFS and SFS, likely due to the differences in solar radiation that affect plant-cover percentage, which in turn affects the arachnid assemblage composition. In addition to 98 arachnid taxa collected and identified to species and morphospecies level, this study resulted in the discovery of two species new to science, which are described as part of this publication (100 arachnid species and 11 additional taxa that were not identified to species, a total of 111 taxa). Our study, moreover, contributes new ecological data on the spatial and temporal distribution of arachnids, and therefore attests to the importance of year-round sampling in an understudied region. Overall, our study enables a better understanding of arachnid diversity and their distributions and serves as a reference for future research aimed at testing the effect of climate change and other environmental factors that influence arachnid assemblages in natural habitats. Full article

(This article belongs to the Special Issue State-of-the-Art Mediterranean Soil Arthropods Diversity)

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22 pages, 1625 KiB

Open AccessEditor’s ChoiceArticle

The Diet of Eleonora’s Falcons (Falco eleonorae) during the Autumn Migration of Passerine Birds across the Aegean Sea

by Dietrich Ristow and Michael Wink

Diversity 2024, 16(9), 538; https://doi.org/10.3390/d16090538 - 2 Sep 2024

Cited by 1 | Viewed by 2410

Abstract

Every year, several hundred million birds cross the Mediterranean on their migration from Eurasia to their wintering quarters in Africa. As many migrants travel at night or at high altitudes, direct observations of bird migration are difficult and thus our information about migrating [...] Read more.

Every year, several hundred million birds cross the Mediterranean on their migration from Eurasia to their wintering quarters in Africa. As many migrants travel at night or at high altitudes, direct observations of bird migration are difficult and thus our information about migrating species, numbers and timing is incomplete. An indirect way to assess autumn migration is the analysis of prey remains of Eleonora’s Falcons (Falco eleonorae). These falcons breed in large colonies on islands in the Mediterranean and on the Canary Islands. Many migrants have to pass these islands on their flight to their African wintering quarters. Eleonora’s Falcons appear to be adapted to the autumn bird migration and raise their young between August and October, when migrating birds are abundant. When nestlings have to be fed, falcons exclusively hunt small birds of 10 to 150 g body mass, whereas they prey mostly on aerial invertebrates (Coleoptera, Hymenoptera, Diptera, Orthoptera, Hemiptera, Odonata, Lepidoptera) from November to July. We studied Eleonora’s Falcons from 1965 to 2001 on a rocky islet, north of Crete, which harboured a colony of about 200 breeding pairs. In 1969, 1971, 1977, and 1988 we systematically monitored and collected the pluckings and cached food items in 22 to 36 nest sites each year. Pluckings were systematically analysed later in Germany using a reference collection of bird feathers for identification. In total, we determined more than 111 prey species (mostly Passerines) comprising more than 13,450 individuals. The top 12 prey species were: Willow Warbler (27.8% of all prey items), Red-backed Shrike (10.7%), Spotted Flycatcher (9.9%), Whinchat (8.8%), Common Whitethroat (5.1%), Wood Warbler (3.8), Tree Pipit (2.9%), Icterine Warbler (2.5%), Greater Short-toed Lark (2.5%), Northern Wheatear (1.8%), Common Nightingale (1.6%), and European Pied Flycatcher (1.5%). Eleonora’s Falcons are selective hunters to some degree; thus, the phenology and abundance data derived from the plucking analyses are biased towards slow-flying species or smaller birds (only up to a body mass of 150 g). When the young falcons develop and grow, food demand increases concomitantly. Comparing the total weight of prey over time indicates a correlation with food demand and in consequence with the number of prey items brought to the nest sites by the falcons. Full article

(This article belongs to the Special Issue 2024 Feature Papers by Diversity’s Editorial Board Members)

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14 pages, 4929 KiB

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Biodiversity as a Tool in the Assessment of the Conservation Status of Coastal Habitats: A Case Study from Calabria (Southern Italy)

by Antonio Morabito, Carmelo Maria Musarella, Giuseppe Caruso and Giovanni Spampinato

Diversity 2024, 16(9), 535; https://doi.org/10.3390/d16090535 - 2 Sep 2024

Cited by 1 | Viewed by 1915

Abstract

The Mediterranean coasts are threatened by human activities that alter habitats structure and functionality, modifying vegetation and causing the loss of typical species. The definition of the conservation status of coastal habitats is essential to preserve these fragile environments through planned policies. This [...] Read more.

The Mediterranean coasts are threatened by human activities that alter habitats structure and functionality, modifying vegetation and causing the loss of typical species. The definition of the conservation status of coastal habitats is essential to preserve these fragile environments through planned policies. This study aims to assess the conservation status of the habitats of community interest (sensu EEC Directive 43/92) through the analysis of biodiversity and correlating it with urbanisation. A total of 73 vegetation relevés were carried out, so allowing 13 revealing different habitats to be identified. The total plant species diversity per habitat was measured by means of the H-index, also used to assess naturalness (N), differently considering native, alien, and disturbance species. To correlate the N index with distance from urban centres, a statistical analysis was performed. The analysis showed the highest values of H⁺ were found in habitats 2240, 2110, 2260, and 2230, while lowest values were observed in habitats 2270* and 2240. The habitats 2270* and 2240, the closest to urban centres, have a lower naturalness score than habitats 1420, 2120, 2250*, and 2270*, where higher naturalness scores have been found and therefore lower levels of disturbance. The criteria and methods discussed in this study can be used in coastal management in order to identify the most sensitive habitats and implement an effective conservation strategy. Full article

(This article belongs to the Special Issue Biodiversity Conservation Planning and Assessment)

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14 pages, 693 KiB

Open AccessEditor’s ChoiceArticle

Exposure of American Black Bears (Ursus americanus) to Ticks, Tick-Borne Diseases, and Intestinal Parasites in Wisconsin

by Nika S. Reichert, Daniela Mathieu, Christopher J. Katz and Kent A. Hatch

Diversity 2024, 16(9), 537; https://doi.org/10.3390/d16090537 - 2 Sep 2024

Cited by 1 | Viewed by 1467

Abstract

We surveyed 159 American black bears (Ursus americanus) over a period of three years for the occurrence of ticks, tick-borne diseases, and intestinal parasites in Wisconsin. We collected blood from the bears to test for the presence of antibodies to Borrelia [...] Read more.

We surveyed 159 American black bears (Ursus americanus) over a period of three years for the occurrence of ticks, tick-borne diseases, and intestinal parasites in Wisconsin. We collected blood from the bears to test for the presence of antibodies to Borrelia burgdorferi (Lyme disease), Rickettsia rickettsii (Rocky Mountain spotted fever (RMSF)), Babesia, Ehrlichia, Ehrlichia canis, Brucella canis, and Anaplasma phagocytophilum. We also examined scat samples for intestinal parasites. We commonly found the tick Dermacentor variabilis, but also present the first report of Rhipicephalus sanguineus on black bears. We detected antibodies to Lyme disease and RMSF. We detected antibodies to E. canis for the first time in a bear and both antibodies to R. rickettsii and A. phagocytophilum for the first time in a black bear in Wisconsin. No antibodies for Babesia or Br. canis were detected. We found eggs of the intestinal parasite Baylasascaris transfuga as well as a low number of Toxascara leonina and unknown Capillaria species occurrences in the examined feces. Full article

(This article belongs to the Special Issue Wildlife Welfare)

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14 pages, 7223 KiB

Open AccessEditor’s ChoiceArticle

Microclimatic Growth Rates of Batrachochytrium salamandrivorans under Current and Future Climates: A Very High Spatial Resolution SDM for Bsal and Salamandra salamandra (Linnaeus, 1758) within Forest Habitats of the European Hotspot Area

by Felix Deiß, Philipp Ginal and Dennis Rödder

Diversity 2024, 16(8), 510; https://doi.org/10.3390/d16080510 - 22 Aug 2024

Viewed by 1808

Abstract

Chytridiomycosis is one of the greatest threats to the diversity of amphibians worldwide. Caused by the chytrid fungus Batrachochytrium salamandrivorans (Bsal), it plays a decisive role in species declines. Bsal is particularly harmful to the European fire salamander (Salamandra salamandra), causing [...] Read more.

Chytridiomycosis is one of the greatest threats to the diversity of amphibians worldwide. Caused by the chytrid fungus Batrachochytrium salamandrivorans (Bsal), it plays a decisive role in species declines. Bsal is particularly harmful to the European fire salamander (Salamandra salamandra), causing ulcerations, anorexia and ataxia, which ultimately lead to death. While most studies have focused on the geographic expansion of the pathogen, there is little high-resolution information available. Therefore, we chose a three-step approach in this study: We (I) used a mechanistic distribution model to project the microclimatic growth rate of Bsal within its invasive range on a spatially very high resolution (25 m). We (II) used a correlative distribution model to predict the potential distribution of S. salamandra and (III) applied n-dimensional hypervolumes to quantify the realized microclimatic niches of both species and examine their overlaps. We estimated future trends based on comparisons among three climate scenarios, the current microclimatic conditions and a +2 °C and +4 °C global mean temperature scenario. We demonstrated that Bsal finds suitable growth conditions everywhere within our study area, thus putting S. salamandra at high risk. However, climate change could lead to less suitable thermal conditions for Bsal, possibly providing a loophole for S. salamandra. Full article

(This article belongs to the Special Issue Application of GIScience and Remote Sensing in Biodiversity Conservation)

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37 pages, 40371 KiB

Open AccessEditor’s ChoiceArticle

Middle Miocene (Langhian and Lower Serravallian/Badenian) Scallops (Bivalvia: Pectinidae) from the Precious Collections of the Croatian Natural History Museum

by Marija Bošnjak, Oleg Mandic and Jasenka Sremac

Diversity 2024, 16(8), 508; https://doi.org/10.3390/d16080508 - 21 Aug 2024

Viewed by 1940

Abstract

The Croatian Natural History Museum (CNHM) houses rich fossil collections from the Neogene deposits of Northern Croatia, comprising numerous scallops (Bivalvia: Pectinidae). During the Middle Miocene (Badenian = Langhian and early Serravallian), this region was located at the southwestern margin of the Central [...] Read more.

The Croatian Natural History Museum (CNHM) houses rich fossil collections from the Neogene deposits of Northern Croatia, comprising numerous scallops (Bivalvia: Pectinidae). During the Middle Miocene (Badenian = Langhian and early Serravallian), this region was located at the southwestern margin of the Central Paratethys. The value of the CNHM’s historical collections has been presented through taxonomic revisions and biostratigraphic, paleoenvironmental and paleogeographic study. Methods included the cross-checking of specimens from museum boxes with all available published data, systematic revision of scallops, recording the abundance of each taxon, defining the preservation state of the specimens, measuring the morphometric elements and taking photographs of each specimen. After the conducted revision of 624 specimens, the number of registered taxa in the collections was reduced from 52 to 33, and their stratigraphic distribution has been updated. The species Lissochlamys excisa (Bronn, 1831) has been recorded for the first time at the southwestern part of the Central Paratethys. The southernmost Badenian record of Delectopecten vitreus (Gmelin, 1791) known so far has been described. Six types of pectinid habitats have been distinguished, based on the provided paleontological and lithological data, also estimating the scallops’ abundance in each of them. Bioerosion and encrustation traces on scallops’ shells represent an additional contribution to paleoecological studies. Full article

(This article belongs to the Special Issue Do We Still Need Natural History Collections?)

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24 pages, 2147 KiB

Open AccessEditor’s ChoiceArticle

The Interaction between Climate Change and Biodiversity Can Be Assessed from a Material Cycle Perspective

by Ah-Young Kim, Who-Seung Lee and Yowhan Son

Diversity 2024, 16(8), 506; https://doi.org/10.3390/d16080506 - 19 Aug 2024

Cited by 1 | Viewed by 3102

Abstract

This study focused on understanding biodiversity variability in response to climate change as an environmental stressor from a carbon cycle perspective. As the frequency and magnitude of environmental stresses are expected to increase due to rising carbon concentrations, these changes may affect the [...] Read more.

This study focused on understanding biodiversity variability in response to climate change as an environmental stressor from a carbon cycle perspective. As the frequency and magnitude of environmental stresses are expected to increase due to rising carbon concentrations, these changes may affect the variability in biodiversity. However, several studies have argued that conventional methods for biodiversity assessment, primarily based on arithmetic indices utilized in national policies, are inadequate for accurately assessing these interactions among species, abiotic changes, and material cycling. Here, we indicate that environmental stressors that occur at various scales and domains, from macroscopic to microscopic, and their effects on ecosystems are multi-dimensional. Furthermore, environmental stresses not only affect organisms inhabiting these ecosystems but these organisms, in return, influence the carbon cycle through life history changes resulting from these stresses. The results of our review suggest that these processes are sequentially interconnected, forming self-reinforcing feedback loops. Ultimately, an integrated approach is necessary to understand biodiversity variability in complex and open ecosystems. This approach should be capable of comprehending changes in the overall flow by considering ecosystem characteristics. Full article

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20 pages, 2440 KiB

Open AccessEditor’s ChoiceArticle

Biodiversity Assessment of a Mississippi River Backwater Complex Using eDNA Metabarcoding

by Eric J. Ludwig, Veronica M. Lee, Leah K. Berkman, Aaron D. Geheber and David D. Duvernell

Diversity 2024, 16(8), 495; https://doi.org/10.3390/d16080495 - 14 Aug 2024

Viewed by 1970

Abstract

The backwater lowland habitats of large rivers, like the Mississippi River in North America, present complex and often inaccessible environments for traditional capture-based fish biodiversity sampling. Our knowledge of the assemblages of the fishes that occupy such habitats is often incomplete, and this [...] Read more.

The backwater lowland habitats of large rivers, like the Mississippi River in North America, present complex and often inaccessible environments for traditional capture-based fish biodiversity sampling. Our knowledge of the assemblages of the fishes that occupy such habitats is often incomplete, and this can compromise management efforts. We employed environmental DNA (eDNA) metabarcoding methods to sample a Mississippi River bottom wetland system to assess the ichthyofaunal diversity and the assemblage structure across habitat types, and we compared our results with capture-based survey records for the same habitats. We collected water samples in the spring and fall of 2022 from slough, ditch, shallow lake, and bayou habitats that varied in depth, vegetation, seasonal variability, and connectivity to the Mississippi River channel. We detected a diverse array of fish species that included 51 taxa. Nearly all the species previously documented in the habitats were detected using eDNA metabarcoding, and we increased the number of documented species by more than a third. Most of the species were ubiquitous across the range of habitats, but there was also a substantial assemblage structure, with some species exhibiting clear habitat specificity. Fall sampling was limited to the deeper bayou habitats where seasonal variation between the spring and fall was minimal. eDNA metabarcode sampling was demonstrated to be effective at detecting invasive species as well as uncommon species, which included several species of conservation concern. Full article

(This article belongs to the Special Issue Applications on Environmental DNA in Aquatic Ecology and Biodiversity)

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16 pages, 15119 KiB

Open AccessEditor’s ChoiceArticle

Skin Anatomy, Bone Histology and Taphonomy of a Toarcian (Lower Jurassic) Ichthyosaur (Reptilia: Ichthyopterygia) from Luxembourg, with Implications for Paleobiology

by Ida Bonnevier Wallstedt, Peter Sjövall, Ben Thuy, Randolph G. De La Garza, Mats E. Eriksson and Johan Lindgren

Diversity 2024, 16(8), 492; https://doi.org/10.3390/d16080492 - 12 Aug 2024

Viewed by 3128

Abstract

A partial ichthyosaur skeleton from the Toarcian (Lower Jurassic) bituminous shales of the ‘Schistes Carton’ unit of southern Luxembourg is described and illustrated. In addition, associated remnant soft tissues are analyzed using a combination of imaging and molecular techniques. The fossil (MNHNL TV344) [...] Read more.

A partial ichthyosaur skeleton from the Toarcian (Lower Jurassic) bituminous shales of the ‘Schistes Carton’ unit of southern Luxembourg is described and illustrated. In addition, associated remnant soft tissues are analyzed using a combination of imaging and molecular techniques. The fossil (MNHNL TV344) comprises scattered appendicular elements, together with a consecutive series of semi-articulated vertebrae surrounded by extensive soft-tissue remains. We conclude that TV344 represents a skeletally immature individual (possibly of the genus Stenopterygius) and that the soft parts primarily consist of fossilized skin, including the epidermis (with embedded melanophore pigment cells and melanosome organelles) and dermis. Ground sections of dorsal ribs display cortical microstructures reminiscent of lines of arrested growth (LAGs), providing an opportunity for a tentative age determination of the animal at the time of death (>3 years). It is further inferred that the exceptional preservation of TV344 was facilitated by seafloor dysoxia/anoxia with periodical intervals of oxygenation, which triggered phosphatization and the subsequent formation of a carbonate concretion. Full article

(This article belongs to the Special Issue Making New Out of the Old: Recent Biological Advances on Mesozoic Marine Reptiles)

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14 pages, 3282 KiB

Open AccessEditor’s ChoiceArticle

Comparative Assessment of Shell Structural, Mechanical, and Elemental Properties in Adult Acorn Barnacles

by Jazmine Shaw, Yeram Kang, Callie Triano, Corin J. Hoppe, Nick Aldred, Rebecca A. Metzler and Gary H. Dickinson

Diversity 2024, 16(8), 482; https://doi.org/10.3390/d16080482 - 8 Aug 2024

Viewed by 1699

Abstract

Balanomorph (acorn) barnacles are found throughout the world’s coastal oceans, and their success is dependent on a hard, mineralized, outer shell. Although macro-scale morphology of barnacle shells has been studied extensively, relatively little is known about shell properties at the micron-scale and if [...] Read more.

Balanomorph (acorn) barnacles are found throughout the world’s coastal oceans, and their success is dependent on a hard, mineralized, outer shell. Although macro-scale morphology of barnacle shells has been studied extensively, relatively little is known about shell properties at the micron-scale and if such properties vary among species. We assessed shell structure, mechanics, and composition in seven species of balanomorph barnacles from five genera. Three species, Amphibalanus amphitrite, Amphibalanus improvisus, and Austrominius modestus, were laboratory-reared, enabling direct comparison of shell properties of barnacles grown under the same conditions for the same duration. Four other species, Semibalanus balanoides, Amphibalanus eburneus, Chthamalus stellatus, and Tetraclita rubescens, were field-collected. At the macro- and meso-scales, shell properties varied markedly among species, with differences in the number of shell plates, the presence of canals within the plates, mineralization of the base, and shell plate thickness. At the micron-scale, however, structure was remarkably similar among species. Plates of all species were constructed of irregular micron-scale crystallites, with a broad range of crystallite dimensions observed within the same shell. Similarly, micromechanical properties did not vary among species, regardless of testing orientation. Calcium carbonate was identified as calcite in all species assessed with no other mineral phases present, and calcium content did not vary among species. Hence, despite variation in the overall macro- and meso-scale morphology of barnacles, all appear to be built using the same, evolutionarily conserved, mineralization pathway. Full article

(This article belongs to the Special Issue Diversity in 2024)

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22 pages, 6248 KiB

Open AccessEditor’s ChoiceArticle

DNA Metabarcoding Analysis of Arthropod Diversity in Dust from the Natural History Museum, Vienna

by Pascal Querner, Nikola Szucsich, Bill Landsberger and Peter Brimblecombe

Diversity 2024, 16(8), 476; https://doi.org/10.3390/d16080476 - 6 Aug 2024

Cited by 1 | Viewed by 2227

Abstract

This paper introduces a new method for identifying museum pests through the analysis of DNA present in settled dust. Traditionally, the identification of pests in cultural institutions such as museums and depositories has relied on insect trapping (monitoring). They give good results but [...] Read more.

This paper introduces a new method for identifying museum pests through the analysis of DNA present in settled dust. Traditionally, the identification of pests in cultural institutions such as museums and depositories has relied on insect trapping (monitoring). They give good results but need time (minimum spring until summer of one year for a complete survey) and face challenges related to the identification of small, rare, or damaged species. Our study presents a non-invasive approach that utilizes metabarcoding analysis of dust samples to identify pests and other arthropods at the species level. We collected dust samples with a handheld vacuum cleaner in summer 2023 from the six different floors of the Natural History Museum in Vienna and compared the results with the insect monitoring. We found over 359 different species of arthropods in the museum and could show how the diversity increases with the elevation of the building floor. This method could be used for rapid and cost-effective screening of pests before monitoring. But the interpretation of results is sometimes difficult (for Lepismatidae, for example), and it cannot replace a continuous monitoring of pests with traps. This investigation might present the highest indoor animal biodiversity ever found in a single building. Full article

(This article belongs to the Special Issue DNA Barcodes for Evolution and Biodiversity—2nd Edition)

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