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Agronomy

Agronomy is an international, peer-reviewed, open access journal on agronomy and agroecology published semimonthly online by MDPI. The Spanish Society of Plant Biology (SEBP) is affiliated with Agronomy and their members receive discounts on the article processing charges.

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All Articles (20,292)

  • Article
  • Open Access

Photoperiod is a crucial environmental factor that regulates plant growth and flowering. While the photoperiodic flowering pathway has been extensively studied, the mechanisms underlying photoperiodic regulation of cotton (Gossypium hirsutum L.) growth and development remain unclear. In this study, we selected two cotton genotypes, XJ12 and XJ21-11, to investigate the effects of long-day (LD) and short-day (SD) conditions on cotton photosynthesis, dry matter accumulation, flowering time, and related gene expression, utilizing physiological index measurements, transcriptomics, and WGCNA. The results indicated that LD treatment significantly enhanced photosynthetic parameters and dry matter weight in both cotton varieties, with the net photosynthetic rate (Pn) being 154.79% and 111.86% higher under LD compared to SD treatment. Additionally, the flowering time of the two varieties was advanced by 12 and 13 days, respectively, under LD treatment relative to SD treatment. Transcriptome analysis revealed that LD treatment upregulated the expression of genes involved in sucrose and starch metabolism, consistent with the enhanced growth observed under LD conditions. In addition, LD treatment altered the expression of genes associated with flavonoid biosynthesis, suggesting changes in secondary metabolism. Differentially expressed genes and co-expression networks were also associated with phytohormone signaling, MAPK signaling, and circadian rhythm-related pathways, with several transcription factors identified as potential regulatory components. Together, these findings suggest a putative regulatory framework in which photoperiod-responsive changes in carbon metabolism, secondary metabolism, circadian regulation, and hormone-related signaling are associated with the coordination of cotton growth and flowering. This study provides candidate pathways and regulatory genes for further investigation of the molecular basis of photoperiodic responses in cotton.

Agronomy

23 September 2026

Effect of different photoperiods on cotton phenotype. (A,B) Comparison of cotton flowering under different photoperiod treatments (taken on the 60th day after treatment). (C) Squaring time and flowering time. (D) Plant height and maximum leaf area. (E) Root dry weight and shoot dry weight. Error bars represent the standard deviation (SD) of the mean. * indicates a significant difference between photoperiod treatments within the same genotype at p < 0.05.
  • Article
  • Open Access

Feasibility of Co-Composting Sewage Sludge with Vine Pruning Residues for Sustainable Waste Management

  • Henda Lopes,
  • Elisabete Nascimento-Gonçalves and
  • Marta Roboredo
  • + 8 authors

The increasing generation of sewage sludge (SS) and the accumulation of agricultural residues such as vine pruning residues (VPR) represent significant environmental challenges, including pollution risks and resource underutilization. Composting offers a sustainable strategy to transform these wastes into high-quality compost, facilitating resource recovery within a circular framework. This study aimed to evaluate the feasibility of co-composting SS with VPR as a strategy for organic waste valorization. Two mixtures were prepared using cattle slurry solid fraction (CS) as inoculum, M1 (50% SS + 30% VPR + 20% CS) and M2 (30% SS + 50% VPR + 20% CS). The results showed that both mixtures exhibited typical composting temperature profiles, with the thermophilic phase effectively eliminating Salmonella and E. coli, confirming adequate hygienization. Macronutrient concentrations were consistently higher in M1 (N: 43.44, P: 12.41, and K: 25.27 g kg−1 DW) than in M2 (N: 32.14, P: 8.57, and K: 23.66 g kg−1 DW). All potentially toxic elements remained below the regulatory thresholds established by Ordinance No. 185/2022. Compost maturity was confirmed in both treatments by final C/N ratios below 20, holocellulose/lignin ratios below 1.8, N-NH4+ concentration below 300 mg kg−1 FW (only M2 satisfied this criterion), and N-NH4+/N-NO3 below 3. However, M2 achieved a very stable respiration rate of 1.95 mg CO2 g−1 OM d−1 and a germination index of 90.22%, while M1 recorded a respiration rate of 2.38 mg CO2 g−1 OM d−1 and a lower germination index of 74%. The findings suggest that while M1 is a superior source of macronutrients, M2 provides a more stable and less phytotoxic organic amendment, with both being safe for agricultural soil application.

Agronomy

23 September 2026

Temperature profile of (a) mixture M1 (replicates 1 and 2) and (b) mixture M2 (replicates 1 and 2), and moisture content (MC) (c). Bars represent means ± SD (n = 2).
  • Article
  • Open Access

Climate change has intensified plant water stress due to rising temperatures and irregular rainfall, which often fails to meet crop water requirements. Consequently, irrigation has become essential for optimizing the yield of drought-sensitive crops such as maize. Drought-tolerant (DT) maize hybrids represent a promising strategy to increase resilience under water-limited conditions. This study evaluated the qualitative, morpho-physiological traits, and yield of six maize hybrids, comprising one drought-tolerant (DT) and one conventional (Conv) hybrid for each of three maturity classes (FAO 300, 400, and 500), in Veneto, northeastern Italy. Hybrids were grown under rainfed and fully irrigated conditions. Irrigated plots received 51.7% more water than the rainfed ones. Irrigation increased plant height by 20.4%. At full development, irrigation raised the plant dry weight by 19.7% and ear weight by 24.7%, relative to rainfed management. At harvest time, the grain yield was 48.7% higher under irrigation than under rainfed conditions. No significant effect of drought tolerance was observed on grain yield. Rainfed maize produced grain with higher protein content. Grain from the DT hybrids had lower protein but higher oil content than grain from the Conv hybrids. Therefore, in environments characterized by high temperatures and irregular but not extremely limiting rainfall, management strategies that secure adequate water supply during the reproductive stages remain more effective for sustaining maize productivity than the sole adoption of DT hybrids. These findings should be confirmed through multi-year, multi-site experiments covering a wider range of water availability conditions.

Agronomy

23 September 2026

The dotted line represents the long-term hydroclimatic balance (HB 2000-23) for the experimental location, constructed using temperature and precipitation data provided by ARPAV and the soil hydrological constants of the experimental site. The area enclosed between the dotted line and the lower limit of readily available water (LL RAW) represents the average duration and intensity of the dry period in the experimental site. WP = wilting point; FC = field capacity.
  • Article
  • Open Access

Flooded rice paddies emit large quantities of greenhouse gases, and biochar amendment serves as a promising strategy to conserve water, sequester carbon, mitigate emissions and stabilize crop yields. Nevertheless, the DNDC (DeNitrification-DeComposition) model has no specific biochar module and fails to precisely quantify coupled water–carbon–nitrogen cycles in biochar-amended paddies. Using two-season field observations from the Jianghan Plain, this study constructs the Biochar-DNDC model by introducing biochar pH and substrate adsorption parameters into the original organic fertilizer module and coupling a double-tank exponential decay model. Five biochar application rates (0, 1.5, 3, 4.5, and 6 kg∙m−2; CK, BC1.5, BC3, BC4.5, BC6) were set. Combined with the 40-year historical precipitation series of the study area, five hydrological year types were classified to carry out multi-scenario simulations. The results indicated that, compared with DNDC, Biochar-DNDC improved the simulation accuracy by an average of 28.4%. In terms of RRMSE, Biochar-DNDC improved the simulation accuracy of yield, SOC, CH4, and N2O by 37.0%, 28.7%, 28.6%, and 42.1%, respectively. Environmental temperature, precipitation, soil bulk density, optimal rice yield, and the proportion of straw returned to the field were key sensitive factors regulating water–carbon–nitrogen fluxes. Biochar reduced irrigation water use by 6.3~28.5% while promoting the accumulation of soil carbon stocks, optimizing nitrogen-use efficiency, and suppressing greenhouse gas emissions. There was a significant interaction between rainfall regime and biochar: DOC and NH4+-N were higher in wet years, while NO3-N tended to accumulate in arid conditions. Biochar weakened the positive driving effect of precipitation on net primary productivity (NPP). Rice yield, NPP, and net ecosystem exchange (NEE) first increased and then decreased with increasing biochar dosage. Overall, the optimal dosage of biochar for water conservation, carbon sequestration, yield increases, and emission reductions in paddy fields on the Jianghan Plain was 4.5 kg∙m−2. This study develops a modeling tool for the carbon and nitrogen cycles in biochar-amended paddy fields, which can provide quantitative support for low-carbon water and fertilizer management in the rice-growing regions of the middle Yangtze River Basin.

Agronomy

22 September 2026

Simulation performance of the models for rice yield.

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Agronomy - ISSN 2073-4395