Journal Description
Agrochemicals
Agrochemicals
is an international, peer-reviewed, open access journal on all aspects of agrochemicals published quarterly online by MDPI.
- Open Access— free for readers, with article processing charges (APC) paid by authors or their institutions.
- High Visibility: indexed within Scopus and other databases.
- Rapid Publication: manuscripts are peer-reviewed and a first decision is provided to authors approximately 27 days after submission; acceptance to publication is undertaken in 3.6 days (median values for papers published in this journal in the second half of 2025).
- Recognition of Reviewers: APC discount vouchers, optional signed peer review, and reviewer names published annually in the journal.
- Agrochemicals is a companion journal of Agronomy.
Latest Articles
Invisible Threats to Food Security: Analysis of Dithiocarbamate Residues in Foods Consumed in Brazil (2001–2023)
Agrochemicals 2026, 5(1), 5; https://doi.org/10.3390/agrochemicals5010005 - 21 Jan 2026
Abstract
This study provides a comprehensive long-term assessment of dithiocarbamate (DTC) fungicide residues in foods consumed in Brazil, analyzing nearly two decades of official monitoring data from the Pesticide Residue Analysis Program (PARA/ANVISA) from 2001 to 2023. By integrating fragmented annual reports into a
[...] Read more.
This study provides a comprehensive long-term assessment of dithiocarbamate (DTC) fungicide residues in foods consumed in Brazil, analyzing nearly two decades of official monitoring data from the Pesticide Residue Analysis Program (PARA/ANVISA) from 2001 to 2023. By integrating fragmented annual reports into a single temporal framework, this study offers a novel evaluation of detection frequencies, residue levels, and regulatory compliance over time. Of the 21,274 samples analyzed, 23.90% contained residues of these fungicides. Papaya showed the highest detection frequency (92.59%) in 2005, while apple showed the highest average percentage of detections (51.68%). Lettuce showed the highest residual levels (10.05 mg kg−1) in samples from the 2017–2018 cycle, despite the lack of authorization for the use of these products in this crop. Strawberries and carrots showed concentrations above the maximum residue limit (MRL), with excesses. Residues of unauthorized pesticides were also detected in crops such as guava, pineapple, and sweet potato. Temporal correlations between detections and residues indicated significant variations among the foods evaluated, with potatoes, strawberries, and lettuce showing the highest residual levels. An overall declining trend in detections and residue concentrations was observed throughout the analyzed period, potentially reflecting improvements in regulatory oversight, agricultural practices, and analytical sensitivity over time. From a public health perspective, the persistence of elevated residues and unauthorized uses highlights the need for continuous surveillance, strengthened enforcement, and risk communication strategies to ensure food safety and consumer protection.
Full article
(This article belongs to the Special Issue Control of Use of Pesticides and Their Impact on Consumer Health)
►
Show Figures
Open AccessReview
A One Health Approach Involving Composting and Compost: Balancing Human Health Risks and Agricultural Benefits
by
Mohamed Ou-Zine, Said El Kinany, Said Ezrari and Rachid Bouamri
Agrochemicals 2026, 5(1), 4; https://doi.org/10.3390/agrochemicals5010004 - 12 Jan 2026
Abstract
The one health approach recognizes the interconnection between human, animal, and environmental health, emphasizing that human health should never be threatened in the pursuit of agricultural productivity. Indeed, within agricultural systems, this approach is particularly relevant, as the overuse of chemical inputs and
[...] Read more.
The one health approach recognizes the interconnection between human, animal, and environmental health, emphasizing that human health should never be threatened in the pursuit of agricultural productivity. Indeed, within agricultural systems, this approach is particularly relevant, as the overuse of chemical inputs and the mismanagement of organic wastes can directly threaten human health. Overuse of chemical inputs can result in various health disturbances and contribute to the development of acute or chronic human diseases. Likewise, organic wastes constitute potential human health risks due to the presence of pathogens in these wastes such as bacteria, viruses, fungi, and parasites. Despite increasing research, many studies often lack integrated risk assessments of agrochemicals and organic waste within a “One Health” framework, leaving gaps in practical guidance for safe agricultural management. This review was conducted to address these gaps and answer the following questions: What are the human health risks associated with agrochemicals and mismanaged organic wastes? How can composting/compost mitigate these risks and support sustainable agricultural production? It examines the role of composting in managing organic wastes, producing high-quality compost, and reducing exposure to hazardous chemicals and pathogens. Furthermore, it outlines key characteristics of compost required to ensure safety for humans, plants, soil, and ecosystems. By integrating evidence on human health and crop productivity, this review provides insights for safe, sustainable agricultural practices within a unified One Health framework.
Full article
(This article belongs to the Section Fertilizers and Soil Improvement Agents)
►▼
Show Figures

Figure 1
Open AccessArticle
Differential Effects of O-Benzyl-Serine on Sulfur Metabolism, Photosynthesis, and Growth in Two Species of Weeds
by
Isabela de Carvalho Contesoto, Ana Paula Boromelo, Ana Paula da Silva Mendonça, Cinthia Martins Corbetta, Amanda Castro Comar, Marco Aurélio Schüler de Oliveira, Larissa Fonseca Tomazini, João Henrique Vieira de Almeida Junior, Marcelo Augusto Batista, Paulo Sérgio Alves Bueno, Caroline Barbeiro, Ana Paula Ferro, Wanderley Dantas dos Santos, Rodrigo Polimeni Constantin, Osvaldo Ferrarese-Filho and Rogério Marchiosi
Agrochemicals 2026, 5(1), 3; https://doi.org/10.3390/agrochemicals5010003 - 9 Jan 2026
Abstract
Enzymes of the sulfur assimilation pathway represent promising candidates for selective herbicide development. This study investigated the effects of O-benzyl-serine (OBS), a newly identified inhibitor of O-acetylserine(thiol)-lyase (OAS-TL), on two C3 weed species, Ipomoea grandifolia and Euphorbia heterophylla. Plants were
[...] Read more.
Enzymes of the sulfur assimilation pathway represent promising candidates for selective herbicide development. This study investigated the effects of O-benzyl-serine (OBS), a newly identified inhibitor of O-acetylserine(thiol)-lyase (OAS-TL), on two C3 weed species, Ipomoea grandifolia and Euphorbia heterophylla. Plants were cultivated hydroponically for 12 days in the presence of OBS (0–500 µM). OBS inhibited root growth in both species in a dose-dependent manner, with I. grandifolia being more sensitive. OAS-TL activity decreased in the roots of I. grandifolia but increased in the leaves of E. heterophylla. Nutrient profiling revealed significant alterations in sulfur, magnesium, and calcium contents, associated with chlorosis and reduced root and leaf development. While photosynthetic performance appeared unaffected at the lowest OBS concentration tested (62.5 µM), higher doses drastically reduced leaf expansion, preventing further measurements. Given this marked decline in foliar development, it is reasonable to infer that the overall photosynthetic capacity of the plants was also negatively affected under severe OBS exposure. OBS also disrupted apical dominance, promoting lateral shoot formation. These findings demonstrate that OBS differentially affects sulfur metabolism and growth in a species- and organ-specific manner, supporting its potential as a prototype molecule for herbicides targeting novel biochemical pathways.
Full article
(This article belongs to the Section Herbicides)
►▼
Show Figures

Figure 1
Open AccessArticle
Compatibility Between Beauveria bassiana and Papain and Their Synergistic Potential in the Control of Tenebrio molitor (Coleoptera: Tenebrionidae)
by
Amanda do Carmo Alves, Ana Carolina Silva, Adriane Toledo da Silva, Nivia Kelly Lima Sales, Ruth Celestina Condori Mamani, Lisseth Bibiana Puentes Figueroa, Elias Honorato Gomes, Debora Castro Toledo de Souza, Rosangela Cristina Marucci and Filippe Elias de Freitas Soares
Agrochemicals 2026, 5(1), 2; https://doi.org/10.3390/agrochemicals5010002 - 2 Jan 2026
Abstract
►▼
Show Figures
The use of proteolytic enzymes in association with entomopathogenic fungi offers a promising alternative for improving the biological control of insect pests. This study evaluated the compatibility between Beauveria bassiana and papain and the effectiveness of their combined application in controlling Tenebrio molitor
[...] Read more.
The use of proteolytic enzymes in association with entomopathogenic fungi offers a promising alternative for improving the biological control of insect pests. This study evaluated the compatibility between Beauveria bassiana and papain and the effectiveness of their combined application in controlling Tenebrio molitor. Conidial viability in the presence of papain was monitored for 48 h and showed a reduction in germination from 100% to approximately 70%, without detrimental effects on fungal performance. Papain activity remained stable up to 12 h, declining afterward, indicating biochemical compatibility. Bioassays revealed significant differences among treatments (p < 0.01). In larvae, mortality ranged from 5.18 ± 0.19% in the control to 49.62 ± 2.00% with papain, 62.24 ± 0.58% with conidia, and 89.71 ± 1.06% in the combined treatment; papain and conidia alone did not differ statistically. In pupae, mortality reached 2.20 ± 0.00% in the control, 47.38 ± 0.69% with papain, 63.69 ± 0.69% with conidia, and 85.91 ± 0.84% with the combination, with all treatments differing significantly. Fungal reisolation confirmed typical B. bassiana development. Overall, the results show that papain does not compromise fungal viability and that its combination with B. bassiana enhances entomopathogenic activity, supporting its potential for integrated pest management.
Full article

Figure 1
Open AccessReview
AI–Remote Sensing for Soil Variability Mapping and Precision Agrochemical Management: A Comprehensive Review of Methods, Limitations, and Climate-Smart Applications
by
Fares Howari
Agrochemicals 2026, 5(1), 1; https://doi.org/10.3390/agrochemicals5010001 - 20 Dec 2025
Abstract
Uniform application of fertilizers and pesticides continues to dominate global agriculture despite significant spatial variability in soil and crop conditions. This mismatch results in avoidable yield gaps, excessive chemical waste, and environmental pressures, including nutrient leaching and greenhouse gas emissions. The integration of
[...] Read more.
Uniform application of fertilizers and pesticides continues to dominate global agriculture despite significant spatial variability in soil and crop conditions. This mismatch results in avoidable yield gaps, excessive chemical waste, and environmental pressures, including nutrient leaching and greenhouse gas emissions. The integration of Artificial Intelligence (AI) and Remote Sensing (RS) has emerged as a transformative framework for diagnosing this variability and enabling site-specific, climate-responsive management. This systematic synthesis reviews evidence from 2000–2025 to assess how AI–RS technologies optimize agrochemical efficiency. A comprehensive search across Scopus, Web of Science, IEEE Xplore, ScienceDirect, and Google Scholar were used. Following rigorous screening and quality assessment, 142 studies were selected for detailed analysis. Data extraction focused on sensor platforms (Landsat-8/9, Sentinel-1/2, UAVs), AI approaches (Random Forests, CNNs, Physics-Informed Neural Networks), and operational outcomes. The synthesized data demonstrate that AI–RS systems can predict critical soil attributes, specifically salinity, moisture, and nutrient levels, with 80–97% accuracy in some cases, depending on spectral resolution and algorithm choice. Operational implementations of Variable-Rate Application (VRA) guided by these predictive maps resulted in fertilizer reductions of 15–30%, pesticide use reductions of 20–40%, and improvements in water-use efficiency of 25–40%. In fields with high soil heterogeneity, these precision strategies delivered yield gains of 8–15%. AI–RS technologies have matured from experimental methods into robust tools capable of shifting agrochemical science from reactive, uniform practices to predictive, precise strategies. However, widespread adoption is currently limited by challenges in data standardization, model transferability, and regulatory alignment. Future progress requires the development of interoperable data infrastructures, digital soil twins, and multi-sensor fusion pipelines to position these technologies as central pillars of sustainable agricultural intensification.
Full article
(This article belongs to the Section Fertilizers and Soil Improvement Agents)
►▼
Show Figures

Figure 1
Open AccessSystematic Review
Agricultural Biomass as a Resource for Biomaterials, Biofertilizers, and Bioproducts: A Systematic Review
by
Bruna Pereira Almeida, Luiz Felipe Silveira Pavão, Marcelo Silveira de Farias, Nidgia Maria Nicolodi, Mirta Teresinha Petry, Marisa Menezes Leal, Paulo Carteri Coradi, Victória Lumertz de Souza, Mayara de Souza Queirós, Guilherme de Figueiredo Furtado, Marcus Vinicíus Tres and Giovani Leone Zabot
Agrochemicals 2025, 4(4), 23; https://doi.org/10.3390/agrochemicals4040023 - 11 Dec 2025
Abstract
This systematic review aimed to examine recent advances (2021–2025) in the conversion of agricultural biomass into biomaterials, biofertilizers, and bioproducts. Studies were included when addressing biomass types, pretreatment methods, conversion technologies, or resulting applications. Non-agricultural biomass, non-original research, and works outside the defined
[...] Read more.
This systematic review aimed to examine recent advances (2021–2025) in the conversion of agricultural biomass into biomaterials, biofertilizers, and bioproducts. Studies were included when addressing biomass types, pretreatment methods, conversion technologies, or resulting applications. Non-agricultural biomass, non-original research, and works outside the defined timeframe were excluded. Literature was identified in Scopus and Web of Science, complemented by Espacenet, Google Scholar, and institutional databases (USDA, FAO, IRRI, ABARES, UNICA, and CONAB, among others), totaling 108 documents referenced in this work. Risk of bias was minimized through predefined eligibility criteria and full-text assessment. Results were narratively synthesized, supported by figures and tables highlighting technological trends. Studies involving a wide range of agricultural biomasses (e.g., rice straw, corn stover, wheat straw, and sugarcane bagasse) were evaluated. Main outcomes included the development of bioplastics, biofoams, composites, hydrogels, bioceramics, biochar-based fertilizers, organic acids, enzymes, and green solvents. Evidence consistently indicated that pretreatment strongly influences conversion efficiency and that enzymatic and thermochemical routes show the highest potential for integrated biorefineries. Limitations included heterogeneity in biomass composition, variability in methodological quality, and scarcity of large-scale studies. Overall, findings underscore agricultural biomass as a strategic feedstock for circular bioeconomy models, with implications for sustainable materials, renewable energy, and low-carbon agriculture. Continued innovation, supportive policies, and improved logistics are essential for scaling biomass-based technologies.
Full article
(This article belongs to the Section Fertilizers and Soil Improvement Agents)
►▼
Show Figures

Figure 1
Open AccessReview
Organophosphate Insecticides: A Brief Overview of Global Use and Their Treatment with Short-Duration Isoflurane
by
Jishnu K. S. Krishnan, John R. Moffett and Aryan M. Namboodiri
Agrochemicals 2025, 4(4), 22; https://doi.org/10.3390/agrochemicals4040022 - 10 Dec 2025
Abstract
Organophosphate (OP) insecticide poisoning remains a significant world health issue. Despite attempts to reduce OP insecticide use in some countries, they continue to be used extensively in many regions, putting agricultural workers at risk of excess exposure. Furthermore, the high toxicity and ready
[...] Read more.
Organophosphate (OP) insecticide poisoning remains a significant world health issue. Despite attempts to reduce OP insecticide use in some countries, they continue to be used extensively in many regions, putting agricultural workers at risk of excess exposure. Furthermore, the high toxicity and ready availability of OP insecticides in agricultural settings have created an additional public health issue due to their use in attempted suicides. Tens of thousands of people are admitted to hospitals every year after intentional ingestion of OP insecticides. The standard treatment regimen for OP poisoning can prevent mortality, even in some severe cases, but these treatments do not protect the central nervous system (CNS) from excitotoxic damage, and therefore, additional neuroprotective treatments are needed. One promising treatment is the use of halogenated ether anesthetics, including isoflurane, a common anesthetic available in hospitals throughout the world. Isoflurane can be administered by inhalation using vaporizer equipment, or it can be injected intravenously as a lipid–water emulsion. In both cases, excellent neuroprotection has been observed in preclinical models, even when administered up to 1 h after the onset of OP insecticide poisoning. Prolonged administration was not necessary for neuroprotective efficacy, with administration times of only 5 min being sufficient. Including inhalational anesthetics as an adjunct to the standard treatment for OP poisoning could significantly reduce chronic morbidities, especially long-term CNS damage. Research is ongoing to bring this promising treatment to human trials.
Full article
(This article belongs to the Section Pesticides)
►▼
Show Figures

Graphical abstract
Open AccessArticle
Patterns, Practices, and Socio-Environmental Dynamics of Pesticide Use in the Horticultural Value Chain: Insights from Smallholder Farmers and Agro-Input Sellers in Iringa and Njombe, Southern Highlands, Tanzania
by
Peter Martin Chilipweli, Elias C. Nyanza and Aiwerasia Vera Ngowi
Agrochemicals 2025, 4(4), 21; https://doi.org/10.3390/agrochemicals4040021 - 3 Dec 2025
Abstract
Background: The use of pesticides among smallholder farmers, agrochemical sellers, and agricultural officers involves a complex interplay of knowledge, economic factors, and regulatory frameworks. Therefore, this study explores the patterns, practices, and socio-environmental dynamics of pesticide use among smallholder farmers and agro-input sellers
[...] Read more.
Background: The use of pesticides among smallholder farmers, agrochemical sellers, and agricultural officers involves a complex interplay of knowledge, economic factors, and regulatory frameworks. Therefore, this study explores the patterns, practices, and socio-environmental dynamics of pesticide use among smallholder farmers and agro-input sellers in Iringa and Njombe. Method: This study employed a qualitative, phenomenological design, guided by the socio-ecological model (SEM), to explore the lived experience of farmers, agro-dealers, and extension officers. It involved a total of 23 interviews performed in the Njombe and Iringa regions. Data were collected between October 2024 and March 2025, using a combination of in-depth phenomenological interviews, key informant interviews, and field observations, and were categorized into themes and subthemes analyzed using InVivo. Results: The study involved a total of 23 participants drawn from the Iringa and Njombe regions. The gender distribution was nearly balanced, with 52.1% male and 47.8% female respondents. The mean age of participants was 33 years (95% CI: 29.3–37.3). In terms of education, over half (52.17%) had completed primary school. The findings show that smallholders in Iringa and Njombe widely use mixed pesticides and fertilizers, rely on trusted brands, and adapt to climate impacts, but face challenges with regard to unsafe mixing, poor storage, fake products, and weak regulation, highlighting the need for better education, market oversight, and safer practices. Conclusion: Using the socio-ecological model, the findings indicate that pesticide use among smallholder horticultural farmers in Iringa and Njombe is influenced by a complex interaction of socio-economic constraints, market forces, climate variability, and institutional shortcomings. Although farmers have some awareness of safe practices, systemic barriers continue to limit the adoption of sustainable pesticide management.
Full article
(This article belongs to the Special Issue Control of Use of Pesticides and Their Impact on Consumer Health)
►▼
Show Figures

Figure 1
Open AccessArticle
Traceable and Biocompatible Carbon Dots from Simple Precursors: A Pre-Deployment Safety Baseline
by
Christian Silva-Sanzana, Plinio Innocenzi, Luca Malfatti, Federico Fiori, Francisca Blanco-Herrera, Juan Hormazabal, María Victoria Gangas, Oscar Diaz and Iván Balic
Agrochemicals 2025, 4(4), 20; https://doi.org/10.3390/agrochemicals4040020 - 20 Nov 2025
Abstract
Carbon dots (CDs) are promising for agro-environmental applications; however, clear connections between synthesis, photophysical properties, size, and biosafety are often not well established. In this study, we map these relationships for glucose–arginine CDs (GA-CDs). By using microwave and hydrothermal routes at precursor ratios
[...] Read more.
Carbon dots (CDs) are promising for agro-environmental applications; however, clear connections between synthesis, photophysical properties, size, and biosafety are often not well established. In this study, we map these relationships for glucose–arginine CDs (GA-CDs). By using microwave and hydrothermal routes at precursor ratios of 1:3, 1:9, and 1:15, we produced sub-10 nm nanoparticles (analyzed by dynamic light scattering and atomic force microscopy) that exhibit tunable absorption and emission properties, as well as surface properties (demonstrated through UV–Vis spectroscopy, 3D photoluminescence, and FTIR analysis). The hydrothermal 1:9 condition yielded the narrowest size distribution and red-shifted photoluminescence. Across biological models spanning plants, insects, plant-growth-promoting bacteria (PGPR), and human cells, GA-CDs were well tolerated, with no adverse changes detected in plant stress markers, aphid feeding behavior or fecundity, or PGPR growth. In A549 cells, viability remained stable up to a concentration of 0.125 mg mL−1, while exposure to 0.5 mg mL−1 reduced viability, establishing a practical operating range. These results provide a clearer picture of how the structure and properties of carbon dots derived from arginine and glucose are correlated to their safety. The GA-CDs are, therefore, useful, and traceable tools for agro-environmental research. The findings support their use as biocompatible nanomaterials for studying interactions among plants, insects, and microbes in agriculture.
Full article
(This article belongs to the Section Fungicides and Bactericides)
►▼
Show Figures

Figure 1
Open AccessArticle
Effects of Selected Biopesticides on Two Arthropod Pests of Cannabis sativa L. in Northeastern Oregon
by
Tiziana Oppedisano, Silvia I. Rondon and Daniel I. Thompson
Agrochemicals 2025, 4(4), 19; https://doi.org/10.3390/agrochemicals4040019 - 26 Oct 2025
Abstract
Hemp (Cannabis sativa L.) cultivation in the United States has expanded rapidly over the past decade. Due to federal and state regulations, only a limited number of studies have examined the chemical options available for controlling pests on C. sativa. In
[...] Read more.
Hemp (Cannabis sativa L.) cultivation in the United States has expanded rapidly over the past decade. Due to federal and state regulations, only a limited number of studies have examined the chemical options available for controlling pests on C. sativa. In the U.S., two of the most important species of arthropod pests affecting C. sativa are the beet leafhopper Circulifer tenellus Baker (Hemiptera: Cicadellidae) and the two-spotted spider mite Tetranychus urticae Koch (Acari: Tetranychidae). This study evaluated the effects of four biopesticides, Chromobacterium subtsugae, Burkholderia spp., Chenopodium ambrosioides, and azadirachtin, under greenhouse conditions against C. tenellus adults and nymphs and T. urticae adults. Biopesticides were applied to foliage using a calibrated hand sprayer. To evaluate the biopesticides’ potency, C. tenellus adults, nymphs, and mites were released 1 h after treatment; to evaluate the residual efficacy, they were released 7 days after treatment (DAT). In both experiments, C. tenellus adults, nymphs, and mites were counted 1, 3, and 7 days after release. Our results indicate that Burkholderia spp. exhibited the highest efficacy against C. tenellus adults at 7 DAT, whereas C. ambrosioides and azadirachtin caused the greatest nymphal mortality at 1 and 3 DAT, respectively. Our results show that Burkholderia spp. had the greatest potency against C. tenellus adults 7 DAT, while C. ambrosioides and azadirachtin highly affect the mortality of nymphs at 1 and 3 DAT, respectively. Treatments with C. subtsugae and C. ambrosioides showed high potency against T. urticae. Finally, C. subtsugae showed the lowest residual effect against the mite pest. The data presented in this article will add to the arsenal of information to improve the current management strategies used against these two hemp pests.
Full article
(This article belongs to the Topic Natural Products in Crop Pest Management)
►▼
Show Figures

Figure 1
Open AccessReview
The Fungal Biorevolution: A Trifecta of Genome Mining, Synthetic Biology, and RNAi for Next-Generation Fungicides
by
Víctor Coca-Ruiz
Agrochemicals 2025, 4(4), 18; https://doi.org/10.3390/agrochemicals4040018 - 14 Oct 2025
Abstract
Modern agriculture faces a critical challenge from escalating fungicide resistance and the ecological impact of conventional agrochemicals. A paradigm shift is required, moving beyond simple product substitution toward an integrated technological platform. This review outlines such a platform, built on the synergy of
[...] Read more.
Modern agriculture faces a critical challenge from escalating fungicide resistance and the ecological impact of conventional agrochemicals. A paradigm shift is required, moving beyond simple product substitution toward an integrated technological platform. This review outlines such a platform, built on the synergy of three technologies: genome mining for rational discovery of novel antifungal compounds, synthetic biology for their scalable and cost-effective production, and RNA interference (RNAi) for highly specific pathogen control and resistance management. We argue that the integration of this trifecta—discovery, production, and targeted application—creates an adaptable pipeline for developing next-generation biofungicides. This approach transforms crop protection from a static defense to a dynamic, sustainable system capable of co-evolving with pathogens, ensuring future food security while minimizing environmental impact.
Full article
(This article belongs to the Section Fungicides and Bactericides)
►▼
Show Figures

Graphical abstract
Open AccessArticle
Natural Herbicide Shows Cytotoxicity, Neurotoxicity, and Antioxidant System Alterations on SH-SY5Y and HaCaT Cell Lines
by
Leticia Nominato-Oliveira, Juliana Ferreira da Silva, Shayane da Silva Milhorini, Larissa Lechinovski, Ana Carolina de Deus Bueno Krawczyk and Izonete Cristina Guiloski
Agrochemicals 2025, 4(3), 17; https://doi.org/10.3390/agrochemicals4030017 - 18 Sep 2025
Abstract
Weeds have acquired resistance to commonly used herbicides, and to replace them, new products, including those of natural origin, have been produced. This study evaluated the neurotoxicity, cytotoxicity, and changes in the antioxidant system caused by Natural Herbicide (NH) in SH-SY5Y neuroblastoma cells
[...] Read more.
Weeds have acquired resistance to commonly used herbicides, and to replace them, new products, including those of natural origin, have been produced. This study evaluated the neurotoxicity, cytotoxicity, and changes in the antioxidant system caused by Natural Herbicide (NH) in SH-SY5Y neuroblastoma cells and HaCaT dermal cells. SH-SY5Y and HaCaT cells were exposed to three concentrations of NH (NH1: 0.6; NH2: 1.56; and NH3: 3.12 µL/mL) for 24 and 72 h. In the SH-SY5Y cell line, the highest concentration of NH (NH3) caused cytotoxicity at both 24 and 72 h. At 24 h, the NH3 group increased the SOD. In the NH2 and NH3 groups, there was also an increase in AChE activity after 24 h of exposure. The NH1 group led to an increase in GSH after 72 h of exposure. As for the HaCaT strain, there was cytotoxicity and an increase in SOD and GSH at all NH concentrations and for both periods analyzed (24 h and 72 h). GST was reduced after exposure to NH2 and NH3. Thus, NH showed cytotoxicity in neural and dermal cells (SH-SY5Y and HaCaT, respectively). These results show that NH altered cellular homeostasis, and the evaluation of other toxicity mechanisms is important to clarify its safety.
Full article
(This article belongs to the Section Herbicides)
►▼
Show Figures

Figure 1
Open AccessArticle
Metabolomics-Driven Investigation of Harpin αβ and Laminarin Effects on Cannabis sativa L. Employing GC/EI/MS and 1H NMR Metabolomics
by
Christos N. Kerezoudis, Maria Zervou, Manolis Matzapetakis, Dimitrios Bilalis and Konstantinos A. Aliferis
Agrochemicals 2025, 4(3), 16; https://doi.org/10.3390/agrochemicals4030016 - 13 Sep 2025
Abstract
Hemp (Cannabis sativa L.) is a polymorphic species that synthesizes an array of bioactive metabolites, with cannabinoids and terpenoids constituting the major chemical classes. Until recently, the lack of legislative framework led to limited research on hemp’s plant protection and nutrition. Biostimulants
[...] Read more.
Hemp (Cannabis sativa L.) is a polymorphic species that synthesizes an array of bioactive metabolites, with cannabinoids and terpenoids constituting the major chemical classes. Until recently, the lack of legislative framework led to limited research on hemp’s plant protection and nutrition. Biostimulants have recently attracted scientific attention as sustainable alternatives to plant protection products (PPPs). Herein, we investigated the effects of biostimulant harpin (αβ) proteins and the PPP polysaccharide laminarin on hemp (cv. Futura 75), employing GC/EI/MS and 1H NMR metabolomics. Analyses demonstrated that treatments induced distinct shifts in the metabolism of the plants, thus, enabling the discovery of metabolite-biomarkers of physiological adaptation, defense mechanisms (α-linolenic acid), and bioactivity (cannabinoids). Harpin and laminarin altered the concentration of bioactive compounds such as cannabidiol, essential amino acids including L-phenylalanine and GABA, salicylate, and caffeate. Pathway analysis revealed treatment-specific modulation of key metabolic networks, with harpin triggering early, yet transient activation of phenylpropanoid- and amino acid-related pathways before broad repression, whereas laminarin maintained a more balanced regulation, sustaining defense-related biosynthesis while preserving core primary metabolism. Results advance the understanding of molecular mechanisms underlying biostimulants’ action in hemp and support their potential for improving plant health and attributes of cannabis-derived products, providing insights for its sustainable cultivation.
Full article
(This article belongs to the Section Plant Growth Regulators and Other Agrochemicals)
►▼
Show Figures

Figure 1
Open AccessArticle
First Identification of P230L and H134R Mutations Conferring SDHIs Resistance in Stemphylium vesicarium Isolated from an Italian Experimental Pear Orchard
by
Katia Gazzetti, Massimiliano Menghini, Irene Maja Nanni, Alessandro Ciriani, Mirco Fabbri, Pietro Venturi and Marina Collina
Agrochemicals 2025, 4(3), 15; https://doi.org/10.3390/agrochemicals4030015 - 29 Aug 2025
Abstract
Since the late 1970s, brown spot of pear (BSP), a fungal disease caused by Stemphylium vesicarium (Wallr.) Simmons, has been one of the most important pear fungal diseases in Italy. To protect orchards from BSP, frequent fungicide application is essential throughout the period
[...] Read more.
Since the late 1970s, brown spot of pear (BSP), a fungal disease caused by Stemphylium vesicarium (Wallr.) Simmons, has been one of the most important pear fungal diseases in Italy. To protect orchards from BSP, frequent fungicide application is essential throughout the period spanning petal fall to the onset of fruit maturation. In Italy, boscalid was the first succinate dehydrogenase inhibitor (SDHIs) fungicide authorised against BSP; subsequently, penthiopyrad and fluxapyroxad were authorised against the disease. In 2016 and 2017, SDHI compounds were applied against BSP as solo products at the University of Bologna’s experimental farm, showing a reduction in efficacy. Stemphylium vesicarium strains were isolated from leaves and fruit, and sensitivity assays and molecular analyses were performed. In vitro tests confirmed resistance to SDHIs, and two specific single-nucleotide polymorphisms were discovered, SDHB P230L and SDHC H134R, both leading to amino acid substitutions in succinate dehydrogenase subunits and confirming the resistant phenotype.
Full article
(This article belongs to the Section Fungicides and Bactericides)
►▼
Show Figures

Figure 1
Open AccessArticle
Weed Control Increases the Growth and Above-Ground Biomass Production of Pinus taeda Plantations in Southern Brazil
by
Matheus Severo de Souza Kulmann, Marcos Gervasio Pereira, Rudi Witschoreck and Mauro Valdir Schumacher
Agrochemicals 2025, 4(3), 14; https://doi.org/10.3390/agrochemicals4030014 - 16 Aug 2025
Abstract
Pinus taeda plantations have been facing declining productivity in South America, especially due to competition for natural resources such as light, water, and nutrients. Competition with spontaneous vegetation in the early years is one of the main constraints on growth and biomass allocation
[...] Read more.
Pinus taeda plantations have been facing declining productivity in South America, especially due to competition for natural resources such as light, water, and nutrients. Competition with spontaneous vegetation in the early years is one of the main constraints on growth and biomass allocation in trees. However, the best method and timing for weed control and its impact on the productivity of Pinus taeda plantations are unknown. This study aims to evaluate whether weed control increases the growth and above-ground biomass production of Pinus taeda plantations in southern Brazil. This study was conducted at two sites with five-year-old Pinus taeda plantations in southern Brazil, with each being submitted to different weed control methods. This study was conducted in randomized blocks, with nine treatments: (i) NC—no weed control, i.e., weeds always present; (ii) PC—physical weed control; (iii) CC–T—chemical weed control in the total area; (iv) CC–R—chemical weed control in rows (1.2 m wide); (v) C6m, (vi) C12m, (vii) C18m, and (viii) C24m—weed control up to 6, 12, 18, and 24 months after planting; and (ix) COC—company operational weed control. The following parameters were evaluated: the floristic composition and weed biomass, height, diameter, stem volume, needle biomass, branches, bark, and stemwood of Pinus taeda. Control of the weed competition, especially by physical means (PC), and chemical control over the entire area (CC–T) promoted significant gains in the growth and above–ground biomass production of Pinus taeda at five years of age, particularly at the Caçador site. The results reinforce the importance of using appropriate strategies for managing weed control to maximize productivity, especially before canopy closure. In addition, the strong correlation between growth variables and the total biomass and stemwood indicates the possibility of obtaining indirect estimates through dendrometric measurements. The results contribute to the improvement of silvicultural management in subtropical regions of southern Brazil.
Full article
(This article belongs to the Section Herbicides)
►▼
Show Figures

Figure 1
Open AccessArticle
Effect of Pre- and Postharvest Chitosan and Calcium Applications on the Yield and Major Biochemical Qualities of Tomato (Lycopersicon esculentum Mill.)
by
Md. Zakir Hossen, S. M. Mashiur Rahman Nayeem, Quazi Forhad Quadir, Shaila Sharmin, Phalguni Das, Tasnuva Jahan Moury, Laila Arafat Sathi, Ronzon Chandra Das and Md. Harun Or Rashid
Agrochemicals 2025, 4(3), 13; https://doi.org/10.3390/agrochemicals4030013 - 11 Aug 2025
Cited by 1
Abstract
Chitosan is an eco-friendly polysaccharide, enhancing growth and managing disease infections in fruits and vegetables. This study examines the effects of preharvest application of chitosan and calcium (Ca) on yield and postharvest chitosan coating on tomato storage. There were nine preharvest treatments, viz.,
[...] Read more.
Chitosan is an eco-friendly polysaccharide, enhancing growth and managing disease infections in fruits and vegetables. This study examines the effects of preharvest application of chitosan and calcium (Ca) on yield and postharvest chitosan coating on tomato storage. There were nine preharvest treatments, viz., T0 = control, T1 = 50 ppm chitosan, T2 = 80 ppm chitosan, T3 = 0.50% Ca, T4 = 1.0% Ca, T5 = T1 and T3 (combined), T6 = T2 and T3 (combined), T7 = T1 and T4 (combined), and T8 = T2 and T4 (combined), and three postharvest treatments, viz., C0 = control, C1 = 0.10% chitosan, and C2 = 0.20% chitosan, to examine the yield parameters and major physical and biochemical qualities of tomatoes on different days after postharvest storage (DAPS). The results revealed that chitosan and Ca treatments had a significant influence on yield while showing an insignificant impact on the biochemical qualities of fresh-harvested tomatoes. Postharvest application of chitosan coatings effectively reduced weight loss and shrinkage (34–37%) compared to the control. At 20 DAPS, only the 0.20% solution met the marketable threshold of ≥5.0, while the control failed in 100% of the samples. As storage duration increased, titratable acid and vitamin C decreased, while lycopene and sugar content rose in tomatoes. This research indicates that foliar spraying with 80 ppm chitosan during fruit initiation significantly boosts tomato yield, and a 0.20% chitosan coating on postharvest tomatoes enhances longevity and preserves biochemical quality.
Full article
(This article belongs to the Section Plant Growth Regulators and Other Agrochemicals)
►▼
Show Figures

Figure 1
Open AccessArticle
Uptake, Distribution, and Activity of Pluronic F68 Adjuvant in Wheat and Its Endophytic Bacillus Isolate
by
Anthony Cartwright, Mohammad Zargaran, Anagha Wankhade, Astrid Jacobson, Joan E. McLean, Anne J. Anderson and David W. Britt
Agrochemicals 2025, 4(3), 12; https://doi.org/10.3390/agrochemicals4030012 - 23 Jul 2025
Cited by 1
Abstract
►▼
Show Figures
Surfactants are widely utilized in agriculture as emulsifying, dispersing, anti-foaming, and wetting agents. In these adjuvant roles, the inherent biological activity of the surfactant is secondary to the active ingredients. Here, the hydrophilic non-ionic surface-active tri-block copolymer Pluronic® F68 is investigated for
[...] Read more.
Surfactants are widely utilized in agriculture as emulsifying, dispersing, anti-foaming, and wetting agents. In these adjuvant roles, the inherent biological activity of the surfactant is secondary to the active ingredients. Here, the hydrophilic non-ionic surface-active tri-block copolymer Pluronic® F68 is investigated for direct biological activity in wheat. F68 binds to and inserts into lipid membranes, which may benefit crops under abiotic stress. F68’s interactions with Triticum aestivum (var Juniper) seedlings and a seed-borne Bacillus spp. endophyte are presented. At concentrations below 10 g/L, F68-primed wheat seeds exhibited unchanged emergence. Root-applied fluorescein-F68 (fF68) was internalized in root epidermal cells and concentrated in highly mobile endosomes. The potential benefit of F68 in droughted wheat was examined and contrasted with wheat treated with the osmolyte, glycine betaine (GB). Photosystem II activity of droughted plants dropped significantly below non-droughted controls, and no clear benefit of F68 (or GB) during drought or rehydration was observed. However, F68-treated wheat exhibited increased transpiration values (for watered plants only) and enhanced shoot dry mass (for watered and droughted plants), not observed for GB-treated or untreated plants. The release of seed-borne bacterial endophytes into the spermosphere of germinating seeds was not affected by F68 (for F68-primed seeds as well as F68 applied to roots), and the planktonic growth of a purified Bacillus spp. seed endophyte was not reduced by F68 applied below the critical micelle concentration. These studies demonstrated that F68 entered wheat root cells, concentrated in endosomes involved in transport, significantly promoted shoot growth, and showed no adverse effects to plant-associated bacteria.
Full article

Figure 1
Open AccessArticle
Essential Oils as Active Ingredients in a Plant-Based Fungicide: An In Vitro Study Demonstrating Growth Inhibition of Gray Mold (Botrytis cinerea)
by
Tyler M. Wilson, Alma Laney, Zabrina Ruggles and Richard E. Carlson
Agrochemicals 2025, 4(3), 11; https://doi.org/10.3390/agrochemicals4030011 - 15 Jul 2025
Cited by 2
Abstract
►▼
Show Figures
The conventional agricultural industry largely relies on pesticides to maintain healthy and viable crops. Application of fungicides, both pre- and post-harvest of crops, is the go-to method for avoiding and eliminating Botrytis cinerea, the fungal pathogen responsible for gray mold. However, conventional
[...] Read more.
The conventional agricultural industry largely relies on pesticides to maintain healthy and viable crops. Application of fungicides, both pre- and post-harvest of crops, is the go-to method for avoiding and eliminating Botrytis cinerea, the fungal pathogen responsible for gray mold. However, conventional fungicides and their residues have purported negative environmental and health impacts. Natural products, such as essential oils, are viewed as a promising alternative to conventional fungicides. The current research is an in vitro study on the antifungal activity of a natural water-based fungicide (N.F.), which uses a blend of essential oils (ajowan, cassia, clove, eucalyptus, lemongrass, oregano) as the active ingredients against B. cinerea. Compared to conventional fungicides tested at the same concentration (50 μL/mL), those with active ingredients of myclobutanil or propiconazole; the N.F. demonstrated significant (F(3,16) = 54, p = <0.001) and complete fungal growth inhibition. While previous research has largely focused on the antifungal properties of single essential oils and/or isolated compounds from essential oils, this research focuses on the efficacy of using a blend of essential oils in a proprietary delivery system. This research is of importance to the fields of agronomy, ecology, and health sciences.
Full article

Figure 1
Open AccessArticle
Optimizing Nitrogen Use Efficiency and Reducing Nutrient Losses in Maize Using Controlled-Release Coated Fertilizers
by
Jong-Hyeong Lee and Hyun-Hwoi Ku
Agrochemicals 2025, 4(3), 10; https://doi.org/10.3390/agrochemicals4030010 - 30 Jun 2025
Cited by 2
Abstract
This study aimed to evaluate the agronomic performance and environmental impact of controlled-release coated fertilizers (CRCFs) in upland maize systems. Specifically, we sought to determine the optimal nitrogen (N) application rate that maximizes nitrogen use efficiency (NUE) and minimizes nutrient runoff, while maintaining
[...] Read more.
This study aimed to evaluate the agronomic performance and environmental impact of controlled-release coated fertilizers (CRCFs) in upland maize systems. Specifically, we sought to determine the optimal nitrogen (N) application rate that maximizes nitrogen use efficiency (NUE) and minimizes nutrient runoff, while maintaining yield comparable to conventional fertilization practices. A two-year field experiment (2017–2018) was conducted to assess CRCF formulations composed of urea, MAP, and potassium sulfate encapsulated in LDPE/EVA coatings with talc, humic acid, and starch additives. Treatments included various nitrogen application rates (33–90 kg N ha−1) using CRCF and a conventional NPK fertilizer (150 kg N ha−1). Measurements included fresh ear yield, aboveground biomass, NUE, and concentrations of total N (TN), nitrate N (NO3−–N), and total P (TP) in surface runoff. Statistical analyses were performed using linear and quadratic regression models to determine yield responses and agronomic optimal N rate. CRCF treatments produced yields comparable to or exceeding those of conventional fertilization while using less than half the recommended N input. The modeled agronomic optimum N rate was 88.4 kg N ha−1, which closely matched the maximum observed yield. CRCF application significantly reduced TN, NO3−–N, and TP runoff in 2017 and improved NUE up to 71.2%. Subsurface placement and sigmoidal nutrient release contributed to reduced nutrient losses. CRCFs can maintain maize yield while reducing N input by approximately 40%, aligning with climate-smart agriculture principles. This strategy enhances NUE, reduces environmental risks, and offers economic benefits by enabling single basal application. Further multi-site studies are recommended to validate these findings under diverse agroecological conditions.
Full article
(This article belongs to the Section Fertilizers and Soil Improvement Agents)
►▼
Show Figures

Figure 1
Open AccessArticle
Do Foliar Fertilizers Promote Increased Productivity of Tropical Grasses?
by
Anna B. O. Moura, Gustavo B. A. Silva, Anna C. C. Paimel, Eildson S. O. Silva, Lucas G. Mota, Camila F. D. Duarte, Carla H. A. Cabral and Carlos E. A. Cabral
Agrochemicals 2025, 4(2), 9; https://doi.org/10.3390/agrochemicals4020009 - 11 Jun 2025
Abstract
►▼
Show Figures
Foliar fertilizers are low-cost agrochemicals used in pastures, and further research is needed regarding their impact on tropical grasses. Therefore, the objective of this research was to evaluate the effects of foliar fertilization on the development of tropical grasses. Two experiments, consisting of
[...] Read more.
Foliar fertilizers are low-cost agrochemicals used in pastures, and further research is needed regarding their impact on tropical grasses. Therefore, the objective of this research was to evaluate the effects of foliar fertilization on the development of tropical grasses. Two experiments, consisting of five treatments and four replicates, were carried out. Each experiment was carried out using the following grasses: Zuri grass (Megathyrsus maximus Jacq. cv. Zuri) and ipypora grass (hybrid of Urochloa brizantha × Urochloa zizizensis). In each experiment, ten treatments were evaluated using a 2 × 5 factorial design with four replications. Treatments combined two soil fertilization strategies (with and without nitrogen) and five foliar fertilization strategies, which consisted of a control treatment without foliar fertilization and four application times: immediately after defoliation (0 leaves) and with 1, 2, and 3 expanded leaves. The grass height, tiller population density (TPD), leaf number (LN), forage dry mass (FDM), individual leaf mass (ILM) were evaluated. In the absence of soil fertilization, foliar fertilizer application had no effect on the development of the grasses (p > 0.05). Foliar fertilization did not affect the FDM of Ipyporã and Zuri grass under any of the conditions evaluated (p > 0.05). When applied in the soil fertilize with nitrogen, foliar fertilizer increased LN by 24% for two grasses (p < 0.05). For Zuri grass, foliar fertilization reduced individual leaf mass by 19% (p < 0.05). Thus, foliar fertilizer does not increase the productivity of tropical grasses, with small effects on the leaf’s appearance in Ipyporã and Zuri grass, without altering the forage mass, which necessitates new studies with agrochemicals, new doses, and concentrations of nitrogen.
Full article

Figure 1
Highly Accessed Articles
Latest Books
E-Mail Alert
News
Topics
Topic in
Agriculture, Agronomy, Crops, Horticulturae, Microorganisms, Plants, Agrochemicals
Applications of Biotechnology in Food and Agriculture
Topic Editors: Edgar Omar Rueda-Puente, Bernardo Murillo-AmadorDeadline: 1 February 2026
Topic in
Agrochemicals, Agronomy, Insects, IJMS, Marine Drugs, Toxins, Agriculture, Biology
Research on Natural Bioactive Product-Based Pesticidal Agents—2nd Edition
Topic Editors: Min Lv, Hui XuDeadline: 28 February 2026
Topic in
Agriculture, Agronomy, Crops, Insects, Plants, Agrochemicals, IJPB
Exploring Plant-Derived Compounds for Effective Insect Pest Management in Agriculture
Topic Editors: Ian Scott, Roselyne M. LabbéDeadline: 31 May 2026
Topic in
Agriculture, Agronomy, Crops, Soil Systems, Agrochemicals
Soil Health and Nutrient Management for Crop Productivity
Topic Editors: Na Li, Dorcas H. FranklinDeadline: 30 September 2026
Conferences
Special Issues
Special Issue in
Agrochemicals
Pesticides and Their Residues: Impacts on Crops, the Environment, and Biodiversity
Guest Editors: Yu Gao, Menglei Xu, Johann ZallerDeadline: 30 June 2026
Special Issue in
Agrochemicals
Current and Next-Generation Phytochemical Agrochemicals: Synergizing AI, Omics, and Synthetic Biology for Sustainable Crop Protection
Guest Editors: Vassya Bankova, Guo-Hua ZhongDeadline: 31 December 2026




