Agrochemicals

Weeds have acquired resistance to commonly used herbicides, and to replace them, new products, including those of natural origin, have been produced. This study evaluated the neurotoxicity, cytotoxicity, and changes in the antioxidant system caused by Natural Herbicide (NH) in SH-SY5Y neuroblastoma cells and HaCaT dermal cells. SH-SY5Y and HaCaT cells were exposed to three concentrations of NH (NH1: 0.6; NH2: 1.56; and NH3: 3.12 µL/mL) for 24 and 72 h. In the SH-SY5Y cell line, the highest concentration of NH (NH3) caused cytotoxicity at both 24 and 72 h. At 24 h, the NH3 group increased the SOD. In the NH2 and NH3 groups, there was also an increase in AChE activity after 24 h of exposure. The NH1 group led to an increase in GSH after 72 h of exposure. As for the HaCaT strain, there was cytotoxicity and an increase in SOD and GSH at all NH concentrations and for both periods analyzed (24 h and 72 h). GST was reduced after exposure to NH2 and NH3. Thus, NH showed cytotoxicity in neural and dermal cells (SH-SY5Y and HaCaT, respectively). These results show that NH altered cellular homeostasis, and the evaluation of other toxicity mechanisms is important to clarify its safety. Full article

Hemp (Cannabis sativa L.) is a polymorphic species that synthesizes an array of bioactive metabolites, with cannabinoids and terpenoids constituting the major chemical classes. Until recently, the lack of legislative framework led to limited research on hemp’s plant protection and nutrition. Biostimulants have recently attracted scientific attention as sustainable alternatives to plant protection products (PPPs). Herein, we investigated the effects of biostimulant harpin (αβ) proteins and the PPP polysaccharide laminarin on hemp (cv. Futura 75), employing GC/EI/MS and ¹H NMR metabolomics. Analyses demonstrated that treatments induced distinct shifts in the metabolism of the plants, thus, enabling the discovery of metabolite-biomarkers of physiological adaptation, defense mechanisms (α-linolenic acid), and bioactivity (cannabinoids). Harpin and laminarin altered the concentration of bioactive compounds such as cannabidiol, essential amino acids including L-phenylalanine and GABA, salicylate, and caffeate. Pathway analysis revealed treatment-specific modulation of key metabolic networks, with harpin triggering early, yet transient activation of phenylpropanoid- and amino acid-related pathways before broad repression, whereas laminarin maintained a more balanced regulation, sustaining defense-related biosynthesis while preserving core primary metabolism. Results advance the understanding of molecular mechanisms underlying biostimulants’ action in hemp and support their potential for improving plant health and attributes of cannabis-derived products, providing insights for its sustainable cultivation. Full article

Since the late 1970s, brown spot of pear (BSP), a fungal disease caused by Stemphylium vesicarium (Wallr.) Simmons, has been one of the most important pear fungal diseases in Italy. To protect orchards from BSP, frequent fungicide application is essential throughout the period spanning petal fall to the onset of fruit maturation. In Italy, boscalid was the first succinate dehydrogenase inhibitor (SDHIs) fungicide authorised against BSP; subsequently, penthiopyrad and fluxapyroxad were authorised against the disease. In 2016 and 2017, SDHI compounds were applied against BSP as solo products at the University of Bologna’s experimental farm, showing a reduction in efficacy. Stemphylium vesicarium strains were isolated from leaves and fruit, and sensitivity assays and molecular analyses were performed. In vitro tests confirmed resistance to SDHIs, and two specific single-nucleotide polymorphisms were discovered, SDHB P230L and SDHC H134R, both leading to amino acid substitutions in succinate dehydrogenase subunits and confirming the resistant phenotype. Full article

Pinus taeda plantations have been facing declining productivity in South America, especially due to competition for natural resources such as light, water, and nutrients. Competition with spontaneous vegetation in the early years is one of the main constraints on growth and biomass allocation in trees. However, the best method and timing for weed control and its impact on the productivity of Pinus taeda plantations are unknown. This study aims to evaluate whether weed control increases the growth and above-ground biomass production of Pinus taeda plantations in southern Brazil. This study was conducted at two sites with five-year-old Pinus taeda plantations in southern Brazil, with each being submitted to different weed control methods. This study was conducted in randomized blocks, with nine treatments: (i) NC—no weed control, i.e., weeds always present; (ii) PC—physical weed control; (iii) CC–T—chemical weed control in the total area; (iv) CC–R—chemical weed control in rows (1.2 m wide); (v) C6m, (vi) C12m, (vii) C18m, and (viii) C24m—weed control up to 6, 12, 18, and 24 months after planting; and (ix) COC—company operational weed control. The following parameters were evaluated: the floristic composition and weed biomass, height, diameter, stem volume, needle biomass, branches, bark, and stemwood of Pinus taeda. Control of the weed competition, especially by physical means (PC), and chemical control over the entire area (CC–T) promoted significant gains in the growth and above–ground biomass production of Pinus taeda at five years of age, particularly at the Caçador site. The results reinforce the importance of using appropriate strategies for managing weed control to maximize productivity, especially before canopy closure. In addition, the strong correlation between growth variables and the total biomass and stemwood indicates the possibility of obtaining indirect estimates through dendrometric measurements. The results contribute to the improvement of silvicultural management in subtropical regions of southern Brazil. Full article

Chitosan is an eco-friendly polysaccharide, enhancing growth and managing disease infections in fruits and vegetables. This study examines the effects of preharvest application of chitosan and calcium (Ca) on yield and postharvest chitosan coating on tomato storage. There were nine preharvest treatments, viz., T0 = control, T1 = 50 ppm chitosan, T2 = 80 ppm chitosan, T3 = 0.50% Ca, T4 = 1.0% Ca, T5 = T1 and T3 (combined), T6 = T2 and T3 (combined), T7 = T1 and T4 (combined), and T8 = T2 and T4 (combined), and three postharvest treatments, viz., C0 = control, C1 = 0.10% chitosan, and C2 = 0.20% chitosan, to examine the yield parameters and major physical and biochemical qualities of tomatoes on different days after postharvest storage (DAPS). The results revealed that chitosan and Ca treatments had a significant influence on yield while showing an insignificant impact on the biochemical qualities of fresh-harvested tomatoes. Postharvest application of chitosan coatings effectively reduced weight loss and shrinkage (34–37%) compared to the control. At 20 DAPS, only the 0.20% solution met the marketable threshold of ≥5.0, while the control failed in 100% of the samples. As storage duration increased, titratable acid and vitamin C decreased, while lycopene and sugar content rose in tomatoes. This research indicates that foliar spraying with 80 ppm chitosan during fruit initiation significantly boosts tomato yield, and a 0.20% chitosan coating on postharvest tomatoes enhances longevity and preserves biochemical quality. Full article

Surfactants are widely utilized in agriculture as emulsifying, dispersing, anti-foaming, and wetting agents. In these adjuvant roles, the inherent biological activity of the surfactant is secondary to the active ingredients. Here, the hydrophilic non-ionic surface-active tri-block copolymer Pluronic^® F68 is investigated for direct biological activity in wheat. F68 binds to and inserts into lipid membranes, which may benefit crops under abiotic stress. F68’s interactions with Triticum aestivum (var Juniper) seedlings and a seed-borne Bacillus spp. endophyte are presented. At concentrations below 10 g/L, F68-primed wheat seeds exhibited unchanged emergence. Root-applied fluorescein-F68 (fF68) was internalized in root epidermal cells and concentrated in highly mobile endosomes. The potential benefit of F68 in droughted wheat was examined and contrasted with wheat treated with the osmolyte, glycine betaine (GB). Photosystem II activity of droughted plants dropped significantly below non-droughted controls, and no clear benefit of F68 (or GB) during drought or rehydration was observed. However, F68-treated wheat exhibited increased transpiration values (for watered plants only) and enhanced shoot dry mass (for watered and droughted plants), not observed for GB-treated or untreated plants. The release of seed-borne bacterial endophytes into the spermosphere of germinating seeds was not affected by F68 (for F68-primed seeds as well as F68 applied to roots), and the planktonic growth of a purified Bacillus spp. seed endophyte was not reduced by F68 applied below the critical micelle concentration. These studies demonstrated that F68 entered wheat root cells, concentrated in endosomes involved in transport, significantly promoted shoot growth, and showed no adverse effects to plant-associated bacteria. Full article

The conventional agricultural industry largely relies on pesticides to maintain healthy and viable crops. Application of fungicides, both pre- and post-harvest of crops, is the go-to method for avoiding and eliminating Botrytis cinerea, the fungal pathogen responsible for gray mold. However, conventional fungicides and their residues have purported negative environmental and health impacts. Natural products, such as essential oils, are viewed as a promising alternative to conventional fungicides. The current research is an in vitro study on the antifungal activity of a natural water-based fungicide (N.F.), which uses a blend of essential oils (ajowan, cassia, clove, eucalyptus, lemongrass, oregano) as the active ingredients against B. cinerea. Compared to conventional fungicides tested at the same concentration (50 μL/mL), those with active ingredients of myclobutanil or propiconazole; the N.F. demonstrated significant (F(3,16) = 54, p = <0.001) and complete fungal growth inhibition. While previous research has largely focused on the antifungal properties of single essential oils and/or isolated compounds from essential oils, this research focuses on the efficacy of using a blend of essential oils in a proprietary delivery system. This research is of importance to the fields of agronomy, ecology, and health sciences. Full article

This study aimed to evaluate the agronomic performance and environmental impact of controlled-release coated fertilizers (CRCFs) in upland maize systems. Specifically, we sought to determine the optimal nitrogen (N) application rate that maximizes nitrogen use efficiency (NUE) and minimizes nutrient runoff, while maintaining yield comparable to conventional fertilization practices. A two-year field experiment (2017–2018) was conducted to assess CRCF formulations composed of urea, MAP, and potassium sulfate encapsulated in LDPE/EVA coatings with talc, humic acid, and starch additives. Treatments included various nitrogen application rates (33–90 kg N ha⁻¹) using CRCF and a conventional NPK fertilizer (150 kg N ha⁻¹). Measurements included fresh ear yield, aboveground biomass, NUE, and concentrations of total N (TN), nitrate N (NO₃⁻–N), and total P (TP) in surface runoff. Statistical analyses were performed using linear and quadratic regression models to determine yield responses and agronomic optimal N rate. CRCF treatments produced yields comparable to or exceeding those of conventional fertilization while using less than half the recommended N input. The modeled agronomic optimum N rate was 88.4 kg N ha⁻¹, which closely matched the maximum observed yield. CRCF application significantly reduced TN, NO₃⁻–N, and TP runoff in 2017 and improved NUE up to 71.2%. Subsurface placement and sigmoidal nutrient release contributed to reduced nutrient losses. CRCFs can maintain maize yield while reducing N input by approximately 40%, aligning with climate-smart agriculture principles. This strategy enhances NUE, reduces environmental risks, and offers economic benefits by enabling single basal application. Further multi-site studies are recommended to validate these findings under diverse agroecological conditions. Full article

Foliar fertilizers are low-cost agrochemicals used in pastures, and further research is needed regarding their impact on tropical grasses. Therefore, the objective of this research was to evaluate the effects of foliar fertilization on the development of tropical grasses. Two experiments, consisting of five treatments and four replicates, were carried out. Each experiment was carried out using the following grasses: Zuri grass (Megathyrsus maximus Jacq. cv. Zuri) and ipypora grass (hybrid of Urochloa brizantha × Urochloa zizizensis). In each experiment, ten treatments were evaluated using a 2 × 5 factorial design with four replications. Treatments combined two soil fertilization strategies (with and without nitrogen) and five foliar fertilization strategies, which consisted of a control treatment without foliar fertilization and four application times: immediately after defoliation (0 leaves) and with 1, 2, and 3 expanded leaves. The grass height, tiller population density (TPD), leaf number (LN), forage dry mass (FDM), individual leaf mass (ILM) were evaluated. In the absence of soil fertilization, foliar fertilizer application had no effect on the development of the grasses (p > 0.05). Foliar fertilization did not affect the FDM of Ipyporã and Zuri grass under any of the conditions evaluated (p > 0.05). When applied in the soil fertilize with nitrogen, foliar fertilizer increased LN by 24% for two grasses (p < 0.05). For Zuri grass, foliar fertilization reduced individual leaf mass by 19% (p < 0.05). Thus, foliar fertilizer does not increase the productivity of tropical grasses, with small effects on the leaf’s appearance in Ipyporã and Zuri grass, without altering the forage mass, which necessitates new studies with agrochemicals, new doses, and concentrations of nitrogen. Full article

Insect frass is the left-over side stream from mass rearing insects as food and feed. Research indicates that black soldier fly, Hermetia illucens, larvae (BSFL) frass can improve the yield of leafy greens while also increasing nutrient uptake. Two studies evaluated the impact of BSFL frass on two Brassicaceae crops: kale (Brassica oleracea) and mustard (Sinapis alba). In Study 1, greenhouse potting mixes comprised of 10% BSFL frass produced kale and mustard fresh and dry weights, relative chlorophyll concentrations, and nitrogen concentration in plant tissues that were comparable to a 100% peat mix control. In mustard tissue, phosphorus and potassium concentrations were higher in the BSFL 10% treatment compared to the control. This provides further motive for incorporating frass into peat-based substrates to reduce peat consumption and extraction. In Study 2, Liquid BSFL frass tea was applied to kale in an outdoor container study. The frass tea only treatment produced the worst outcomes for yield. However, a mixture of frass tea and traditional fertilizer resulted in comparable yield to a control provided the same volume in solely fertilizer. With further research, frass tea could be supplemented to reduce conventional fertilizers. Full article

Large weed infestation is a major problem in dry direct-seeded rice (DSR). Chemical weed control serves as a crucial component for integrated weed management in DSR. Over the last decade, herbicide use has increased from 42 to 55%, and the worldwide contamination of water resources and food by herbicides is a major health issue. In the present study, the use of herbicides in three different establishment methods of rice was examined with the objective to present and discuss the herbicide use pattern and cost of weed control. For this, a field-wide survey was conducted over an area of 165.4 ha in eight villages of the Tarn Taran District of Punjab, India. For two DSR methods, during the initial stage of crop growth, the weed infestation was reported to be less in moist fields sown with direct seeding (soil moisture in the field capacity stage) after pre-sowing irrigation (DSR-PSI). The herbicide use and cost of weed control under DSR-PSI conditions were similar to that of puddled transplanted rice, but were significantly lower than that of direct seeding in dry fields (rice seeds are sown in dry fields, and irrigation is applied immediately after sowing), i.e., DSR-IAS. Therefore, the DSR-PSI method of rice establishment can ensure minimum dependence on herbicides, as well as other benefits of direct seeding. Thus, there is a need to promote the DSR-PSI method over the DSR-IAS method among farmers in order to reduce herbicide use in DSR and ensure environmental safety. Full article

Sustainable fertilization using local resources such as manure is crucial for soil health. This study evaluated the potential of guinea pig manure to replace mineral fertilizers in hard yellow maize (hybrid INIA 619) under Peruvian coastal conditions. A split-plot design tested four doses of guinea pig manure (0, 2, 5, 10 t⋅ha⁻¹) and four levels of mineral fertilization (0%, 50%, 75%, 100%). The study assessed plant height, ear characteristics, yield, and nutritional quality parameters. The results indicated that 100% mineral fertilization led to the highest plant height (229.67 cm) and grain weight (141.8 g). Yields of 9.19 and 9.08 t⋅ha⁻¹ were achieved with 5 and 10 t⋅ha⁻¹ of manure, while 50% mineral fertilization gave 8.8 t⋅ha⁻¹, similar to the full dose (8.7 t⋅ha⁻¹). The protein content was highest with 10 t⋅ha⁻¹ of manure combined with mineral fertilization. However, no significant differences were found between the 50%, 75%, and 100% mineral fertilizer doses. In conclusion, applying guinea pig manure improved nutrient use efficiency, yield, and grain protein quality in maize, reducing the need for mineral fertilizers by up to 50%. This provides a sustainable fertilization strategy for agricultural systems. Full article

Wheat pathogens pose a significant risk to global wheat production, with climate change further complicating disease dynamics. Effective management requires a combination of genetic resistance, cultural practices, and careful use of chemical controls. Ongoing research and adaptation to changing environmental conditions are crucial for sustaining wheat yields and food security. Based on selective academic literature retrieved from the Scopus database and analyzed by a bibliographic software such as the VOSviewer we discussed and focused on various aspects of current and future strategies for managing major wheat pathogens and diseases such as Tan spot, Septoria tritici blotch, Fusarium head blight, etc. Chemical management methods, such as the use of fungicides, can be effective but are not always preferred. Instead, agronomic practices like crop rotation and tillage play a significant role in managing wheat diseases by reducing both the incidence and severity of these diseases. Moreover, adopting resistance strategies is essential for effective disease management. Full article

This study explores consumer perceptions of the Greek food system, focusing on safety concerns related to pesticide residues. Utilizing a qualitative research design, thematic analysis was conducted on data collected from 1024 participants through an online survey platform between May and November 2024. Participants, representing diverse demographics across Greece, provided insights into their experiences and concerns regarding food safety. The analysis revealed significant themes, including a crisis of confidence in governance, demands for transparency, and skepticism towards food system actors. Participants expressed disillusionment with the state’s role in ensuring food safety and highlighted the need for a governance framework that aligns with community values. The findings underscore the importance of empowering consumers with accurate information to foster informed decision-making and rebuild trust in the food system. Ultimately, this study emphasizes the necessity for a transformative approach to food governance that incorporates diverse voices and perspectives, aiming to create a more equitable and sustainable food system in Greece. These insights contribute to the broader discourse on food citizenship and the collective responsibilities of all stakeholders in ensuring food safety and integrity. Full article

Seed enhancements involve post-harvest modifications that improve germination and plant performance. One form of enhancement involves coatings, which encompasses encrusting, pelleting, and film coats. These coatings may contain agrichemicals, such as fungicides and insecticides, and can foster conformational changes that improve the plantability of small or irregularly shaped seeds. Seed encapsulation using pharmaceutical capsules can be viewed as an extension of seed coatings where seeds and other beneficial agrichemicals can be combined into a single plantable unit. For many crops, direct contact with high levels of conventional fertilizers may induce some level of phytotoxicity, and early studies involving fertilizer-enriched seed coatings resulted in decreased seedling emergence and diminished plant performance. Encapsulation, however, provides greater delivery volumes compared to other coatings and may offer some degree of separation between seeds and potentially phytotoxic agrochemicals. This study considered tomato seed encapsulation with controlled-release fertilizers. In general, seed exposure to gelatin-based capsules delayed germination by 2- to 3- days. Nevertheless, seed encapsulation improved plant performance including increased plant height and dry mass production by as much as 75 and 460%, respectively. These growth responses mitigated any effects attributed to germination delays. Moreover, higher levels of controlled-release fertilizers (≥800 mg) fostered earlier flower induction by up to 3 weeks. Collectively, the results suggest that seed encapsulation can be an effective way to deliver fertilizers to plants in a manner that could reduce overall fertilizer application rates and possibly lessen the quantity of plant nutrient input necessary for tomato cultivation. Full article

It is debated whether the ecotoxicity of active substances (ASs) contained in synthetic pesticides applied in conventional agriculture (conASs) differs from nature-based ASs used in organic agriculture (orgASs). Using the official pesticide use statistics, we evaluated the ecotoxicity of ASs used in apple and grapevine production in Austria. In 2022, 49 conASs and 21 orgASs were authorized for apple production and 60 conASs and 23 orgASs were authorized for grapevine production in Austria. Based on the latest publicly available data on the actual use of pesticides in apple and grapevine production (from the year 2017), we evaluated their ecotoxicity based on information in the freely accessible Pesticide Properties and Bio-Pesticides Databases. The results showed that although the amount of ASs applied per hectare of field was higher in organic farming, the intrinsic toxicities of ASs used in conventional farming were much higher. The number of lethal toxic doses (LD₅₀) of ASs applied in conventional apple orchards was 645%, 15%, and 6011% higher for honeybees, birds, and earthworms, respectively, than in organic apple production. In conventional vineyards, lethal doses for honeybees, birds, and earthworms were 300%, 129%, and 299% higher than in organic vineyards. We conclude that promoting organic farming would therefore contribute to the better protection of biodiversity on agricultural land and beyond. Full article

Industrial hemp (Cannabis sativa) production is complex, with strict regulatory constraints and challenges associated with a lack of labeled pesticides due to its status as a novel crop in the US. Four experiments were conducted in 2020 and 2021 to establish herbicide tolerances for hemp production in the coastal plain of Georgia, USA. Objectives included evaluating hemp response to pretransplant or posttransplant herbicides, determining if planting method influenced herbicide injury from residual preplant applied herbicides, and understanding how plastic mulch may influence hemp flower yields. When applied one day prior to transplanting, maximum hemp crop visual injury was less than 12% compared to the untreated control, with acetochlor, flumioxazin, fomesafen, pendimethalin, and norflurazon while dithiopyr, halosulfuron, isoxaben, and isoxaflutole resulted in greater than 50% injury. Posttransplant applications of S-metolachlor, acetochlor, pendimethalin, and clethodim resulted in less than 15% injury while halosulfuron, metribuzin, trifloxysulfuron, imazethapyr, and prometryn applications resulted in greater than 50% injury to plants. Preplant and posttransplant applied herbicides were found to have little effect on total tetrahydrocannabinol (THC), cannabidiol (CBD), or total cannabinoids in the dry flower after harvest. In a separate experiment, injury from halosulfuron and metribuzin was 52% to 56% less when planted with a mechanical transplanter as compared to the practice of using a transplant wheel to depress a hole in the soil followed by hand transplanting. In the final experiment, hemp dry flower yield in a non-plastic mulched (bareground) system was similar to that in a plastic mulched system. However, early season plant above-ground biomass was less in the plastic mulched system, which may have been due to elevated soil temperatures inhibiting early season growth. Full article