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Grasses

Grasses is an international, peer-reviewed, open access journal on all fundamental and applied fields of grass, published quarterly online by MDPI.

All Articles (109)

Selection of Morphoagronomic Traits for Screening Tropical Forage Genotypes for Waterlogging Tolerance Under Controlled Conditions

  • Clemeson Silva de Souza,
  • Marcio de Oliveira Martins and
  • Giselle Mariano Lessa de Assis
  • + 4 authors

Poorly drained pastures in tropical America are recurrently degraded by Marandu Death Syndrome (MDS), affecting beef and dairy production. This study screened genotypes of Megathyrsus maximus and Urochloa spp. for waterlogging tolerance under controlled conditions to identify discriminant, easily measurable morphoagronomic traits suitable for breeding programs. Four experiments were conducted in factorial arrangement (five genotypes × two water regimes, with four replications), where morphoagronomic and physiological variables were analyzed using multivariate techniques. The first two principal components explained 75.17–88.60% of the total variation and stayed above 70% after variable reduction, without significantly altering genotype dispersion. Physiological responses showed a strong correlation with morphoagronomic traits. The most informative traits were the number of yellow and senescent leaves, number of tillers, SPAD index, leaf dry mass, and root dry mass. Genotypes were grouped by tolerance level. Among M. maximus, ‘Mombaça’ was the most tolerant, while PM13 and PM21 were the least. In Urochloa spp., U. humidicola cv. Tully was the most tolerant and ‘Marandu’ the least tolerant. Screening under controlled conditions is an alternative to distinguish genotypes with contrasting tolerance; however, because controlled environments do not fully reproduce the multifactorial nature of MDS, this approach is recommended only for early stages of breeding programs. Nevertheless, field evaluations on poorly drained soils under grazing remain essential to confirm tolerance to MDS.

15 January 2026

Daily means of temperature (°C), relative air humidity (%), and vapor pressure deficit (VPD, kPa) inside the screen house at Embrapa Acre: Experiment 1 with M. maximus (a); Experiment 2 with M. maximus (b); Experiment 3 with Urochloa spp. (c); Experiment 4 with Urochloa spp. (d).

Grasses of Campos Rupestres: Diversity, Functions and Perspectives for Seedling Production and Ecological Restoration

  • Alessandra Rodrigues Kozovits,
  • Maurílio Assis Figueiredo and
  • Maria Cristina Teixeira Braga Messias

The Campos Rupestres, ancient and nutrient-poor mountaintop ecosystems in Brazil, harbor exceptional biodiversity and endemism but face severe threats from mining and urban expansion. Native grasses (Poaceae), represented by nearly 300 documented species—many of them poorly studied—are fundamental elements of these ecosystems. They provide critical ecological services, including soil stabilization, enhancing carbon storage and nutrient cycling, regulating water availability, and resilience to disturbances. This review synthesizes current knowledge on the diversity, functions, and propagation of Campos Rupestres grasses, with emphasis on their potential in ecological restoration. Despite their ecological importance, large-scale use of native grasses remains incipient, constrained by limited knowledge of reproductive biology, low seed viability, and scarce commercial seed availability. Advances in propagation include seedling and plug production, vegetative propagation, and rescue/reintroduction strategies, which have shown promising results in post-mining restoration. However, reliance on seed collection from natural populations risks depleting already limited genetic resources, highlighting the need for ex situ production systems. Expanding research on taxonomy, ecology, and cost-effective propagation methods, alongside supportive policy and market development, is crucial for integrating native grasses as cornerstone species in restoration programs. Bridging these gaps will enhance biodiversity conservation and restoration in one of the world’s most threatened megadiverse systems.

13 January 2026

Minimum (grey) and maximum (black) germination percentages obtained in various experiments with native grass species from Campos Rupestres in Brazil. The numbers following the species names represent the assessments conducted for each species and considered in this study. Evaluations that included germination in darkness, seed scarification with H2SO4, germination stimulation with KNO3, or seed preheating were not considered to determine the minimum and maximum germination, since depending on the species, these treatments can reduce germinability. For further details, and citation of the studies from which the data were taken, see Supplementary Materials Table S2.
  • Feature Paper
  • Article
  • Open Access

Functional properties of coastal halophytes are important for development of salt-tolerant cash crop cultures. The study of salt tolerance in coastal dune-building grass Leymus arenarius holds significant importance for its application in land reclamation, soil stabilization, and enhancing crop resilience to salinity stress. We used two accessions (LA1 and LA2) of L. arenarius to compare effects of salinity caused by NaCl and NaNO3 on growth, ion accumulation and mineral nutrition in controlled conditions. L. arenarius plants exhibited high tolerance to sodium salts, with distinct effects on growth and development observed between chloride and nitrate treatments. While both salts negatively impacted root biomass, nitrate treatment (50–100 mmol L−1) increased leaf number and biomass in LA2 plants, whereas chloride treatment decreased tiller and leaf sheath biomass. Despite individual variations, salinity treatments showed comparable effects on traits like tiller and leaf count, as well as leaf blade and sheath biomass. Salinity increased water content in leaf blades, sheaths, and roots, with LA2 plants showing the most pronounced effects. Chlorophyll a fluorescence measurements indicated a positive impact of NaNO3 treatment on photosynthesis at intermediate salt concentrations, but a decrease at high salinity, particularly in LA2 plants. The accumulation capacity for Na+ in nitrate-treated plants reached 30 and 20 g kg−1 in leaves and roots, respectively. In contrast, the accumulation capacity in chloride-treated plants was significantly lower, approximately 10 g kg−1, in both leaves and roots. Both treatments increased nitrogen, phosphorus, and manganese concentrations in leaves and roots, with varying effects on calcium, magnesium, iron, zinc, and copper concentrations depending on the type of salt and tissue. These findings highlight the potential of L. arenarius for restoring saline and nitrogen-contaminated environments and position it as a valuable model for advancing research on salt tolerance mechanisms to improve cereal crop resilience.

7 January 2026

Number of tillers (A), number of leaves (B), dry biomass of leaf blades (C), dry biomass of leaf sheaths (D), dry biomass of rhizomes (E), and dry biomass of roots (F) of Leymus arenarius plants from different accessions (LA1 and LA2) under the effect of increasing concentration of NaCl and NaNO3 in substrate. Data are means ± SE from five replicates. Different letters indicate statistically significant differences according to the Tukey HSD test (p < 0.05).

Drought-Resilience in Mexican Drylands: Integrative C4 Grasses and Forage Shrubs

  • Ma. Enriqueta Luna-Coronel,
  • Héctor Gutiérrez-Bañuelos and
  • Francisco Javier Gutiérrez-Piña
  • + 3 authors

Grassland-based livestock systems across Mexico’s arid and semi-arid belt are increasingly exposed to drought, degrading forage reliability, and soil function. This review synthesizes evidence on native C4 grasses and forage shrubs as complementary building blocks of drought-resilient swards. We searched Web of Science, Scopus, CAB Abstracts and key grey sources (USDA/NRCS Plant Guides, USFS FEIS, Tropical Forages, SNICS) for 1990–2025 studies in English/Spanish. Dominant native grasses (Bouteloua spp., Hilaria belangeri, Digitaria californica, Trichloris crinita, Sporobolus airoides, Panicum hallii) provide high warm-season digestibility and structural cover via C4 physiology, basal/intercalary meristems, and deep/fibrous roots. Forage shrubs (Atriplex canescens, Desmanthus bicornutus, Leucaena leucocephala, Flourensia cernua, Prosopis spp.) bridge the dry-season protein/energy gap and create “resource islands” that enhance infiltration, provided anti-nutritional risks (mimosine/DHP, tannins, salts/oxalates, terpenoids) are managed by dose and diet mixing. We integrate these findings into a Resistance–Recovery–Persistence framework and translate them into operations: (i) site-matching rules for species/layouts, (ii) PLS (pure live seed)-based seed specifications and establishment protocols, (iii) grazing TIDD (timing–intensity–distribution–duration) with a practical monitoring dashboard (CP targets, stubble/cover thresholds, NDVI/SPEI triggers). Remaining bottlenecks are seed quality/availability and uneven extension; policy alignment on PLS procurement and regional seed increase can accelerate adoption. Mixed native grass–shrub systems are a viable, scalable pathway to strengthening drought resilience in Mexican rangelands.

6 January 2026

Resistance–Recovery–Persistence (R–R–P) framework summarizing the mechanisms through which native C4 grasses and forage shrubs contribute to drought resilience in Mexican drylands. Arrows denote the hypothesized causal pathways from management interventions and forage components to ecosystem processes and drought-resilience outcomes across drought and recovery phases.

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Grasses - ISSN 2813-3463