Grasses

Historical sheep farming in the Patagonian drylands has led to reduced grass cover, soil erosion, and shrub encroachment, compromising ecosystem function. Effective restoration requires managing shrub cover, bare soil, and patch connectivity through various strategies. This study evaluates rehabilitation interventions in a grass-steppe ecosystem, comparing grazed and ungrazed areas. Over three years, we tested the following: (a) mechanical shrub cutting with biomass redistribution, and (b) enhancing patch connectivity with Pinus spp. branch piles, alongside controls, in eighteen 5 m × 5 m plots invaded by Mulinum spinosum. Half of the plots were fenced to exclude grazing, resulting in six treatment combinations. We monitored soil properties, vegetation cover, and species composition. The treatments explained twice as much of the variation in community composition as the annual climatic variations (0.26 vs. 0.13). Livestock exclusion increased perennial grass cover more than the grazed plots did (2.14 vs. 1.42 times the initial measure). All treatments reduced the amount of bare soil except the grazed controls. Shrub cutting, especially with grazing, increased the lasting litter coverage by 5–10% and decreased the bare soil equivalently. Organic matter increased except in the non-intervened interpatches (0.95 times). The enclosures with cut shrubs trapped erodible particles, showing a 5% increase. Our study highlights that grazing destabilizes communities, while enclosures stabilize them, with interventions improving soil fertility and mitigating erosion. Full article

Melatonin (MT) is a multifunctional hormone that enhances crop resilience against various abiotic stresses. However, its regulatory mechanism of osmotic tolerance in forage oats (Avena sativa) plants under water-limited scenarios is still unclear. This study aimed to delineate the impact of MT pretreatment on the morphological, physiological, and biochemical functions of oat seedlings under osmotic stress. Our findings demonstrated that exogenous treatment of MT noticeably elevated leaf area while decreasing the root/shoot ratio of oat seedlings subjected to osmotic stress. Osmotic-induced 38.22% or 48.37% decrease in relative water content could be significantly alleviated by MT pretreatment on day 7 or day 14, respectively. MT treatment also significantly mitigated osmotic-induced decreases in photosynthetic parameters including net photosynthetic rate, stomatic conductance, and intercellular CO₂ concentration as well as various chlorophyll fluorescence parameters, which could contribute to enhanced accumulations of free proline and soluble sugars in seedlings after being subjected to a prolonged duration of osmotic stress. Furthermore, MT markedly improved antioxidant enzyme activities including superoxide dismutase, ascorbate peroxidase, catalase, and peroxidase along with the accumulation of ascorbic acid contributing to a significant reduction in reactive oxygen species under osmotic stress. In addition, the MT application induced a 978.12%, 33.54%, or 30.59% increase in endogenous MT, indole acetic acid, or gibberellic acid content under osmotic stress but did not affect the accumulation of abscisic acid. These findings suggest that an optimal concentration of MT (100 μmol·L⁻¹) could relieve osmotic stress via improvement in osmotic adjustment, the enzymatic antioxidant defense system, and endogenous hormonal balance, thereby contributing to enhanced photosynthetic functions and growth of oat seedlings under water-limited conditions. Full article

This study was carried out to characterize the dynamics of forage accumulation during the regrowth of Mulatto grass submitted to rotational grazing strategies. The treatments corresponded to combinations between two pre-grazing conditions (95% and a maximum light interception during regrowth—LI_95% and LI_Max) and two post-grazing conditions (post-grazing heights of 15 and 20 cm), according to a 2 × 2 factorial arrangement and randomized complete block design, with four replications. Rates of leaf growth (LGR), stems growth (SGR), total growth (TGR), leaf senescence (LSR), grass accumulation (GAR) (kg·ha⁻¹·day⁻¹), and the senescence/canopy growth ratio during different stages of regrowth. There was no difference between the management strategies for TGR. However, a higher GAR was reported for pastures managed with LI_95% relative to LI_Max, of 161.7 and 120.2 kg DM ha⁻¹·day⁻¹, respectively. Pastures managed with LI_95% have a lower SGR in the intermediate and final regrowth period, reflecting the efficient control in the stalks production. On the other hand, in pastures managed, the LI_Max showed higher SGR and LSR in the final regrowth phase. Thus, the LAI was higher in pastures managed at LI_95% compared to those managed at LI_Max, of 163.9 and 112.7 kg DM ha⁻¹·day⁻¹, respectively. Mulatto grass pastures, which were managed at LI_95% pre-grazing, corresponded to approximately 30 cm in height, showed higher LAI, and ensured a low SGR throughout the regrowth period, constituting a more efficient management strategy. Full article

In a continued search for legumes to grow with forage sorghum (FS) [Sorghum bicolor (L.) Moench] in semi-arid environments, studies in 2019 and 2022 at New Mexico State University Rex E. Kirksey Agricultural Science Center at Tucumcari, NM USA, evaluated FS mixed with cowpea [Vigna unguiculata (L.) Walp.], lablab (Lablab purpureus L.), both previously evaluated, and a native legume, big-pod sesbania (Sesbania macrocarpa), for yield and nutritive value in four randomized complete blocks each year. At harvest legume proportions of approximately 15% of the dry matter yield, there were no differences in the yield (mean = 15.97 Mg ha⁻¹, p > 0.40) or land equivalency ratio between sole FS and any mixture or among mixtures; however, the crude protein of FS+Lablab was greater than sole FS, with FS+Cowpea and FS+Sesbania intermediate (67, 77, 87, and 79 g kg⁻¹ for sole FS, FS+Cowpea, FS+Lablab, and FS+Sesbania, respectively; 5% LSD = 14). The neutral detergent fiber digestibility of FS+Sesbania was less than all other treatments (p = 0.0266). Although the sesbania did not improve forage yield or nutritive value when grown with FS and harvested near sesbania maturity, perhaps growing with a shorter season grass and harvesting earlier may show benefit, as sesbania’s nutritive value is known to be greater at earlier stages. Full article

Nitrogen fertilization is one of the main management strategies for continuous pasture management with high productivity. We examined the effects of nitrogen fertilization on the morphogenic, structural, and tillering dynamic characteristics of Tanzania grass in the Amazon region in the state of Pará, Brazil. The study was conducted using a randomized block design with six treatments (0, 100, 200, 300, 400, and 500 kg N ha⁻¹ year⁻¹) and five replicates. The treatments were performed during the rainy season in 2016 and 2017 using urea as the nitrogen source. The leaf elongation rate was increased by 68.5% compared with that of the control treatment (p < 0.01). The leaf appearance rate and number of alive leaves increased with higher doses of nitrogen (p < 0.01, each). The regrowth period was reduced by approximately 13 days under 500 kg N ha⁻¹ (p < 0.01), thus providing more production cycles. Nitrogen fertilization was also associated with a higher tillering rate (p < 0.01); however, the rate of this increase decreased with increasing nitrogen dose. Higher nitrogen doses thus improved the development of Tanzania grass; however, this became less pronounced at doses < 300 kg N ha⁻¹. Full article

In the United States, rangelands comprise 30% of the total land cover and serve as a valuable resource for livestock, wildlife, water, and recreation. Rangelands vary in climate and are often subject to disturbances like drought and wildfire. Historic wildfire trends have indicated an increase in wildfire size and frequency, raising societal and ecological concerns about the management of these lands, both pre- and post-wildfire. While there has been investigation into the effects of grazing prior to a wildfire on fire severity and plant mortality, there is limited research related to grazing post-wildfire even though current management paradigms suggest deferring grazing rangeland for two years after a wildfire to avoid additional stress on native plant species. Based on the diversity found across rangeland ecotypes and history with wildfire, the two-year deferment recommendation may need to be reconsidered for some ecosystems. Species found in perennial bunchgrass rangelands like Pseudoroegneria spicata (bluebunch wheatgrass) and Festuca idahoensis (Idaho fescue) may be less susceptible to post-fire grazing than initially thought, necessitating the need for research into different rangeland ecosystems. Full article

The focus of our study was the changes in the composition of semi-natural dry grasslands in Hungary. Maintaining the favorable condition of grasslands is not only important from a theoretical nature conservation point of view, but it also has important economic implications. Since these valuable habitats were created with the help of humans, their preservation also requires active treatment. Our current experiment was aimed at investigating the suppression of tall grass, Calamagrostis epigejos L. Roth. In Hungary, in the Cserhát Mountains, eight permanent plots were mown twice a year. We surveyed the vegetation twice a year between 2001 and 2011. The effects of treatment were studied with repeated measures analysis of variance (ANOVA). After 10 years, the C. epigejos cover of the mown plots decreased significantly, from the initial average of 62.38 to 7.50%. Surprisingly, we noticed a decrease in the control plots as well. While percentage cover of C. epigejos decreased in all plots, the decrease was significantly stronger in the mown plots. Regular treatment caused an increase in the number of species and diversity. Species richness increased continuously in both treatment types, which indicates the combined effect of vegetation succession and treatment. The biomass growth of other Poaceae and Fabaceae species, which are important from a grassland management perspective, was also facilitated by mowing. Our results allow us to conclude that long-term regular mowing is recommended for preservation from the perspective of the richness and variety of grassland management functional groups and the functioning of the ecosystem in semi-arid regenerating grasslands. Full article

Arid and semi-arid areas are highly productive ecosystems that have a great diversity of species, among which grasses stand out. These species have ecological, environmental, and economic importance. The objective was to identify the ecological descriptors of 15 native species of grasses and diversity patterns through multivariate analysis techniques (principal component analysis, PCA, and cluster analysis, CA), in addition to identifying potential distribution areas (current and future) and regions of high and low diversity from 3841 accessions and 21 climatic variables. Among the main results, the extreme values, coefficient of variation, and median for each species could be determined. PCA determined that, with 10 variables, it was possible to explain 54.36% of the variation between the analyzed species. CA resulted in the formation of four statistically significant groups, with specific climatic characteristics. Regarding potential distribution areas and climate change, a reduction in distribution is expected (2050) on most of the current surface, with a possible opportunity zone in the north of the country. Finally, two hotspots (high diversity areas) and four coldspots (low diversity areas) were identified among the analyzed species. These results can be used to create strategies for sustainable use and the conservation of these resources. Full article

Forage yield estimates provide relevant information to manage and quantify ecosystem services in grasslands. We fitted and validated prediction models of forage yield for several prominent grasses used in restoration projects in semiarid areas. We used field forage harvests from three different sites in Northern Utah and Southern California, USA, in conjunction with multispectral, high-resolution UAV imagery. Different model structures were tested with simple models using a unique predictor, the forage volumetric 3D space, and more complex models, where RGB, red edge, and near-infrared spectral bands and associated vegetation indices were used as predictors. We found that for most dense canopy grasses, using a simple linear model structure could explain most (R² 0.7) of the variability of the response variable. This was not the case for sparse canopy grasses, where a full multispectral dataset and a non-parametric model approach (random forest) were required to obtain a maximum R² of 0.53. We developed transparent protocols to model forage yield where, in most circumstances, acceptable results could be obtained with affordable RGB sensors and UAV platforms. This is important as users can obtain rapid estimates with inexpensive sensors for most of the grasses included in this study. Full article

Grasslands cover approximately 40% of the Earth’s surface. Thus, they play a pivotal role in supporting biodiversity, ecosystem services, and human livelihoods. These ecosystems provide crucial habitats for specialized plant and animal species, act as carbon sinks to mitigate climate change, and are vital for agriculture and pastoralism. However, grasslands face ongoing threats from certain factors, like land use changes, overgrazing, and climate change. Geospatial technologies have become indispensable to manage and protect these valuable ecosystems. This review focuses on the application of Google Earth Engine (GEE) in grasslands. The study presents a bibliometric analysis of research conducted between 2016–2023. Findings from the analysis reveal a significant growth in the use of GEE and different remote sensing products for grassland studies. Most authors reported grassland degradation in most countries. Additionally, China leads in research contributions, followed by the United States and Brazil. However, the analysis highlights the need for greater involvement from developing countries, particularly in Africa. Furthermore, it highlights the global distribution of research efforts, emphasizes the need for broader international participation. Full article

Gelatin industry residues are increasingly used as fertilizer and soil conditioner. However, correct residue dosage is critical for grass development and minimizing environmental impacts. This randomized block design study determined adequate dosage of mixed gelatin sludge (MGS) for Marandu grass production in wet/dry seasons in Brazil. Five MGS levels (0–200% of required nitrogen) were compared to mineral fertilizer. Agronomic/productivity characteristics, bromatological composition, macro/micronutrient composition of leaves, and soil chemical attributes were evaluated. Agronomic/productivity characteristics were influenced by MGS dose in both dry/rainy seasons, except for leaf blade pseudostem ratio and percentage of leaves/pseudostem. Bromatological composition was influenced by MGS doses in dry/rainy seasons except for dry/mineral material quantities. Marandu leaf tissue chemical composition was significantly influenced by MGS dose, except for potassium, boron, and iron. Chemical composition of four soil layers between 0 and 50 cm influenced MGS dose, except for pH, organic matter, magnesium, copper, manganese, and zinc. GMS dose for Marandu production should be 200% of nitrogen requirement. MGS application increased productivity/quality of Marandu grass. Macronutrients (nitrogen, phosphorus) and micronutrients (calcium, magnesium, sulfur, copper, and zinc) increased in Marandu grass and in the soil (calcium, sulfur, and sodium). The increased sodium level was not limiting. Full article

The high-Andean grasslands of Peru provide a wide range of goods and services, not only locally, but also regionally and globally. However, land-use change and global warming are threatening these ecosystems, of which soil organic carbon (SOC) is a key element affecting their sustainability. In this study, we have analyzed the variation of SOC stocks to a depth of 20 cm in 16 paired cropland and grassland sites located in the Sullccapallcca stream micro-watershed (elevation > 3600 m.a.s.l., Ayacucho, Peru). We have also analyzed the environmental controls on the SOC stocks and their variation with land-use change. We found that the studied high-Andean grasslands store high SOC contents (247 Tn SOC ha⁻¹), whose spatial variability was partially explained by the slope of the terrain (r² = 0.26, p < 0.05). Despite the higher NDVI, the conversion of these grasslands into croplands decreased the SOC stock by 39 Tn SOC ha⁻¹ on average, a decrease that was more pronounced when the initial SOC content of the grassland was higher (r² = 0.60, p < 0.05). This study provides the first evidence of the effects of land-use change on the SOC in the region, although the mechanisms involved still need to be investigated. Full article

In Brazil, grazing is the main or only source of food for livestock. The appropriate combination of supplementation with concentrate in a lamb’s diet on pasture is an alternative that can be explored to use natural resources to produce quality meat. The aim of the current study was to evaluate the effects of different supplementation levels (0%, 1.5%, and 3% of BW) on the intake, performance, and production costs of lambs grazing on Aruana (Megathyrsus maximus) and Marandu (Brachiaria brizantha) grasses. Thirty-six non-castrated male Suffolk lambs (22.54 ± 2.72 kg) were used. The lambs were evaluated for nutrient intake and digestibility, such as dry matter (DM), crude protein (CP), neutral detergent fiber (NDF), acid detergent fiber (ADF), and consumption in relation to body weight (% BW), in addition to the average daily gain (ADG), body condition score (BCS), and hot carcass weight (HCW), as well as the rising production cost of each system. The dry matter of the green leaf blades was influenced by the pasture type (p < 0.05), producing 1503.6 vs. 2977.4 kg/ha of Aruana and Marandu grasses, respectively. The concentrate supplementation level influenced the type of pasture (p < 0.05). A higher consumption of DM, CP, NDF, and organic matter was registered for the supplemented animals (p < 0.05) and on Aruana grass. The intake in relation to body weight was significantly influenced by the concentrate levels (p < 0.05). The empty body weight and HCW were significantly influenced by the supplementation levels (p < 0.05). The ADG and feed conversion (FC; kg DM/ADG) were influenced by the supplementation levels and type of pasture. The BCSs differed between the concentrate levels (p < 0.05). The supplementation improved dry matter digestibility. The ADG and FC were superior in the supplemented animals, with an advantage for those grazing on Aruana grass. The slaughter ADG was also higher in the supplemented animals. The lambs’ pasture comprising Aruana grass with 1.5% BW of concentrate supplementation showed improved production and economic results. Full article

The objective was to characterize the nutrient and mineral composition of M. maximus cv Aruana grazed by lambs in a silvopastoral system or full sun with added nitrogen. The treatments are: (1) Aruana grazed in full sun with nitrogen fertilization, (2) Aruana grazed in full sun without nitrogen fertilization, (3) Aruana grazed in a silvopastoral system with nitrogen fertilization and (4) Aruana grazed in a silvopastoral system without nitrogen fertilization. The nutrient dynamics, urinary excretion of N and the amount returned of the nutrient were determined from four sheep in a Latin square experimental design; the analysis of the mineral composition of the leaves and stalks of the grass was carried out by collecting samples from the plots used, totaling six in each treatment evaluated. The silvopastoral system provided high levels of P and K in forage plants. Urinary N excretion and urine mineral contents were influenced by the systems evaluated and the use of N fertilizer. N did not show differences for leaves and canes. Full article

Hybrid Bermuda grasses (Cynodon dactylon × C. transvaalensis Burtt.-Davy) represent one of the greatest contributions to the growing quality of turfgrass in the warm season and transition zone areas of the world. Hybrid Bermuda grass production relies on vegetative propagation from sod or sprigs. In the past, efforts have focused on improving the technique of stolonizing (or sprigging) for establishment in new areas. Such propagation requires bulk harvesting and planting of all rhizomes and stolons. We have developed a novel method of propagation and establishment from a single node harvested from greenhouse grown stolons. Despite a stolon fraction bearing a single node being suitable for effectively propagating a warm-season turfgrass, the technique has been held as economically impractical until now. Our method has been developed to obtain the multiplication of plant material in soilless conditions by harvesting single-node sprigs, propagation of plants from the single nodes, and transplant of single plants in the field. The investigation aimed to identify values for method set-up. Indeed, node and internode size variability with differential between maximum diameters is crucial for discrimination. For Patriot Bermuda grass stolons, nodes exhibited a maximum diameter of 2.43 ± 0.46 mm, while internodes had a maximum diameter of 1.54 ± 0.16 mm. Based on these findings, a 2 mm sieve was selected, achieving an optimal ratio between the node fraction and internode residues. The sieve yielded 87% of node fractions and only 1% of internodes from the initial mix, demonstrating its efficacy. Further results for the transplanting phase indicated that a double release resulted in an average success rate of 98.8%, with only 6.9% blank cells when using a single release. The average was 149 plants per tray over 160 cells, representing a 93.1% success rate. These results underscore the efficiency and acceptability of the overall propagation process in alignment with market references. Full article

Meadow fescue, Schedonorus pratensis (Huds.) P. Beauv., has recently been discovered as a common but previously unknown pasture grass in the Driftless Area of the upper Mississippi River Valley, USA. Preliminary data also indicated that many meadow fescue pastures were infected with an endophytic fungus, Epichloë uncinata (W. Gams, Petrini & D. Schmidt) Leuchtm. & Schardl. Therefore, the objective of this study was to determine if the endophyte impacts agronomic fitness of the host meadow fescue. Meadow fescue plants from eight farm sites were intensively sampled, and endophyte infection levels were determined to range from 82 to 95%. Paired endophyte-infected (E+) and endophyte-free (E−) meadow fescue subpopulations from each collection site were then created, and were subsequently compared for greenhouse and field drought tolerance, forage mass, and persistence under frequent defoliation. There was no impact of the endophyte under a wide range of drought conditions for either greenhouse or field studies. Furthermore, there was a small forage-mass-enhancement effect in the E+ subpopulation for only one of the eight collection sites. The only consistent effect was an average of 9% increased ground cover (persistence) in endophyte-infected meadow fescue under frequent defoliation. As per other studies, enhanced root growth, fungal-disease resistance, and/or reduced insect feeding could be mechanisms for this increased survivorship. We conclude that the meadow fescue endophytes present in the Driftless Area do not help protect their host from drought or provide any consistent forage-growth enhancement; however, we found evidence that the endophyte provides some protection against frequent defoliation at low residual sward heights. Full article

The goals of a “restoration” programme are often not clearly defined, which may radically influence the course of the change effected by the “restoration”. In this paper, I examine many restoration studies worldwide, the goals and the practice that was followed. Grasses often play an important role in both the succession and restoration processes. In Johannesburg, South Africa, wind-blown dust caused by gold mining was a problem in the early 20th century, so the goal was to stabilise the tailings. This was achieved by establishing vegetation on the dumps or revegetation of the land on which the tailings were deposited. Stabilisation of the Cape Flats was the goal in the late 19th century to allow easy movement along sandy roads in the region and marram grass was introduced from Europe specifically for this purpose. Revegetation with a different vegetation type is often a logical option where land use is of paramount importance. Replacement with crop plants or grasses is where the previous vegetation is replaced by an alternative land use that will be of benefit to the local people. The terms “rehabilitation” and “restoration” are often used interchangeably. Early researchers on the subject pointed out that rehabilitation is on the trajectory to full restoration or the original former state of the degraded site. In this review, the rehabilitation processes are discussed following dune mining in South Africa and at sites in Mozambique. Full article

The Nama Karoo biome is one of the least well-studied biomes in the semi-arid region of South Africa, and essential baseline biodiversity data for this region are lacking. The aim of the present study was to examine the influence of environmental factors on the species diversity and richness of Mountain Zebra National Park, South Africa, which includes this vital biome. Vegetation data were obtained using the step-point method. Both species richness and diversity were unaffected by slope, aspect, coarse fragments, and soil texture. Multiple linear regression analyses indicated that a combination of four variables (nitrogen, clay, and sand contents, and longitude) should be included in the optimal model for species richness, and the optimal model for species diversity also revealed four influencing variables: soil organic carbon, clay and sand contents, and longitude. Overall, both species richness and diversity could be predicted by a combination of climatic, topographic, and soil properties. The findings of this study can be used as a reference for the effects of environmental factors on plant species richness and diversity in semi-arid environments. Full article

Supplementing tropical grasses with leguminous forages is known to improve the nutritive value of forage-based diets. However, it is not clear how basal grass forages supplemented with Kudzu (Pueraria phaseoloides) affect the nitrogen (N) and crude protein (CP) fractions of grass/legume forages. The aim of this study was to determine the N and CP fractions and in vitro ruminal CP degradability of Brachiaria hybrid (B. ruziziensis × B. decumbens × B. brizantha) and elephant grass (Pennisetum purpureum) supplemented with Kudzu at a target CP content of 133 ± 0.6 g/kg of dry matter (DM). The grass/legume forages were evaluated for total N, buffer-soluble N (BSN), buffer-insoluble N (BISN), non-protein nitrogen (NPN), neutral detergent-insoluble N (NDIN), acid detergent-insoluble N (ADIN), CP fractions A, B₁, B₂, B₃, C, rumen-degradable protein (RDP), rumen-undegraded protein (RUP), and in vitro ruminal CP degradability. The CP concentration of Kudzu was 217 g/kg DM, while grass forages ranged between 79.9 and 112 g/kg DM. The BISN, CP degradability parameters b, and potential degradability were approximately 56, 41, and 74%, respectively, higher in grass forages supplemented with Kudzu. The concentrations of RUP in the grass forages (23.9–32.5 g/kg DM) were significantly improved when they were supplemented with Kudzu (72.0–79.9 g/kg DM). Therefore, we concluded that basal grass forages supplemented with Kudzu to a target CP content can improve the amount of RUP supplied by the forage portion of the diet. This can have a positive effect on forage utilization and animal performance while reducing the cost to feed for ruminants. Full article