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Grasses

Grasses is an international, peer-reviewed, open access journal on all fundamental and applied fields of grass, published quarterly online by MDPI.

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All Articles (146)

  • Article
  • Open Access

Does Carbon-Based Material Influence Tropical Grasses Under Water Deficit?

  • Carlos E. A. Cabral,
  • Eildson S. O. Silva and
  • Mario R. S. Soares
  • + 7 authors

Carbon-based materials (CM) have been investigated for their potential to improve plant responses to abiotic stresses, including water deficit, but their effects on tropical forage grasses remain poorly understood. The objective of this study was to assess whether CM affects the productivity of tropical grasses under water deficit. Two experiments were conducted, each with cultivars that are hybrids of Urochloa spp.: Sabiá grass and Cayana grass. The study was carried out in a greenhouse using four treatments arranged in a 2 × 2 factorial design. Treatments consisted of two water availability conditions (with and without water deficit) and two strategies (with and without application of CM). Evaluations were performed over two growth cycles. For Cayana grass, forage production was not affected by water deficit, and CM did not increase forage production. In contrast, water deficit impaired the development of Sabiá grass, and CM application increased forage mass regardless of water availability and maintained a lower phyllochron under water deficit. Carbon-based material increased forage production in Sabiá grass but not in Cayana grass, indicating that their effects may depend on the grass genotype and the conditions under which water deficit occurs.

Grasses

16 October 2026

Chlorophyll index of Cayana grass subjected to CM application and water deficit. Means followed by the same uppercase letter within CM and the same lowercase letter within water deficit do not differ according to Tukey’s test (p > 0.05).
  • Feature Paper
  • Article
  • Open Access

Accurate estimation of individual herbage intake in grazing ruminants is essential for improving feeding efficiency and animal performance, yet it remains challenging under field conditions. This study evaluated the association between live weight dynamics recorded by an automated walk-over-weighing (WoW) system and equation-derived herbage dry matter intake (HDMI) in dairy ewes and whether this association changed throughout lactation. The study was conducted over two years (2023–2024) using 92 Sarda dairy ewes managed under part-time grazing with two daily pasture access times (2 or 6 h day−1). Live weight was recorded daily before and after pasture access, and daily live weight change was averaged weekly. HDMI was estimated using a published prediction equation based on milk yield, herbage neutral detergent fibre concentration, and pasture access time. Live weight change was partitioned into between-animal (DW_mean) and within-animal (DW_dev) components, allowing persistent differences among animals to be distinguished from short-term within-animal fluctuations, and analysed using linear mixed-effects models. In the pooled model, both components were positively associated with equation-derived HDMI (p < 0.001), while the significant DW_dev × days-in-milk interaction (p = 0.004) indicated that the association between within-animal live weight change and equation-derived HDMI decreased as lactation advanced. However, pasture access-time-specific sensitivity analyses showed that the associations with DW_mean and DW_dev persisted within AT-6h but were not detected within AT-2h, while adjustment for milk yield attenuated the within-animal association, which was no longer statistically significant, and also removed statistical support for the DW_dev × days-in-milk interaction. The mixed-effects model explained a substantial proportion of the variability in equation-derived HDMI (marginal R2 = 0.64; conditional R2 = 0.94), and equation-derived and model-fitted HDMI were positively correlated (r = 0.77). These findings indicate that WoW-derived live weight dynamics provide non-invasive information associated with equation-derived HDMI under the conditions of this study, but independent validation against directly measured individual herbage intake is required before their use for individual intake estimation.

Grasses

14 October 2026

Marginal association between within-animal live weight change (DW_dev) and equation-derived herbage dry matter intake (HDMI) across lactation. The relationship is derived from the interaction between DW_dev and days in milk (DIM) estimated by the final mixed-effects model. The dashed lines indicate the model-derived zero-crossing (approximately 176 DIM), corresponding to the DIM at which the estimated marginal association between DW_dev and equation-derived HDMI becomes zero. This value should not be interpreted as a physiological threshold.
  • Article
  • Open Access

Seasonal Dynamics of Biomass Recovery in Amelichloa clandestina Under Non-Selective Grazing in North-Eastern Mexico

  • Sait Juanes Márquez,
  • Perpetuo Álvarez Vázquez and
  • Antonio Flores-Naveda
  • + 5 authors

The objective of this study was to evaluate the regrowth capacity of Amelichloa clandestina at different regrowth intervals after non-selective grazing in different seasons of the year. The study was carried out on a 2.23 ha−1 site in northeastern Mexico. Plots of 600 m2 were used, with three replications, and were subjected to grazing at 333 LU ha−1 at the beginning of every season, with biweekly evaluations. Dry matter yield (DMY) was determined by harvesting 1 m2 per plot and extrapolating it to kg ha−1. Morphological composition was evaluated from a 10% subsample of the total biomass. The height of 12 plants and light interception were measured with a ruler and a Quantum meter, respectively. The data were analysed using a randomized complete block design (RCBD) with a split-plot in time structure, evaluating Season (S), Days After Regrowth (DAR), and their interaction (S × DAR). Means were compared using the Tukey test in JMP 14 Pro. In autumn, DMY peaked at 535.0 kg ha−1 at 90 DAR (p ≤ 0.05), accompanied by a maximum canopy height of 28.7 cm and a light interception of 30.3% at the same interval. Leaf biomass was highest in autumn, with a seasonal mean of 220.8 kg ha−1 across all regrowth intervals. A significant correlation was found between plant height and light interception (r = 0.67, p ≤ 0.001). Autumn favoured A. clandestina productivity under a high stocking rate.

Grasses

13 October 2026

Geographic location of the study area in northeastern Mexico. Prepared by the author using QGIS 3.36.3 (Sait Juanes-Márquez).
  • Article
  • Open Access

Pasture-based livestock systems in Argentina’s Humid Pampas are increasingly confined to soils with shallow or mechanically restrictive horizons, limiting forage production even under favourable climates. Tall wheatgrass [Elymus elongatus (Host) Runemark] is one of the few forage species capable of enduring these environments. To better understand forage yield drivers, we assessed the impact of soil restrictions, water, and nitrogen availability. Shoot biomass, water and nitrogen uptake, and water and radiation use efficiency (WUE, RUE) were evaluated during autumn, spring, and summer over two years. Experimental factors included edaphic environment (differing in water-holding capacity and A-horizon thickness), water availability (rainfed vs. irrigated to potential evapotranspiration), and nitrogen (0 vs. 300 kg N ha−1). Yield was challenged by the edaphic environment even under non-limiting resource supply. Water was clearly linked to yield only during summer stressful conditions, while nitrogen uptake was ever present through its impact on canopy development, thereby influencing WUE and RUE. This study demonstrates that forage yield is fundamentally constrained by the edaphic environment, with the magnitude of this response aligning with soil harshness and climatic drivers of yield.

Grasses

30 September 2026

Location of the experimental sites and panoramic view of the tall wheatgrass pastures evaluated during the study. Map background from NASA POWER Data Access Viewer, https://power.larc.nasa.gov (accessed on 21 September 2026).

Featured Articles of Last Quarter

Principal component analysis (PCA) loading plot of chemical and nutritional variables measured in fresh forages (f) and their corresponding silages (s). The first two principal components explained 76.2% of the total variance (PC1 = 49.5%, PC2 = 26.7%). Lines connecting fresh forage (f) and silage (s) loadings were included to facilitate visualization of compositional changes associated with the ensiling process. The figure represents variable loadings projected onto the first two principal components rather than sample scores.
Morphological variation in leaf shape among the ten sweet potato accessions evaluated in this study. The accessions represent genetically distinct materials from the UNIVASF Active Germplasm Bank and illustrate the phenotypic diversity underlying differences in agronomic performance, fermentation characteristics, and nutritional composition.

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Grasses - ISSN 2813-3463